MD00G1004000.v1.1

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000220
Physical Location & Seq
Forward (+)
649640 .. 651174
1535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1004000.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGTCTCCTACTGAATGGTGGATCATGTATGGGACCGATGCACCAACTGTGAGAAAGTTAGCAATAAAAGTATTATCACAAACGGCTTCCTCATCTGCTTGTGAAAGAAATTGGAGCACATTTGCACTCATCCACACAAAGCAAAGAAATAAGTTGGCTCATAGTAGCTTGGAAAAATTAGTTTATTGCTACTACAACATGAAGCTTCAAATTCGAGATAAGGAAGCAGAAATCGATCATGTCGACCGTGGTGACCCACTAGATGTGTTTGATATTGTTGGTGAAGATGATGATACGGAGGGTAACCAACTTTTTCAATGGATTAGACCTCTTCATTTAGATGATGATGAAGGCAACCCAGCTCCCAGAGTTGCTGAAGAAGCACGTAATGAAGGGATAAATGTAGAAAGAGTATTAGAGGAGGAGGTGGGATCTAGCAGCACTGACTCTTTCGAAGAACTTTTGCAGCCAAGACCAAGCAACACTGGAATTCCACATTTTTCCAATCCTACACAACCACAACATCGTGCTGATACTAATGATAGCTCTAGTACAAGATCAGGAGACTCACCTACCACCGGAGGTGGGAATGATGAAGGACATAGTGGAGCTGGAGGTAGTGGAGCTGGAGGTAGTGGTGGTGGATATGGAAACTATTATGGACCACCACCTCCCGGATATATGAGCCCCTTCACTGGTGAGGCAAACTTCACGCATGCAACCCAGGATGATGACCATGGCAGTAGGCGGGCAGGACCAGGAATTGGTGCCATAGGGAAGGACTATACTCGCAGAGAAAGAGGCAAAAAGATTTTGTCAAGTCAAGAAGATGACTTGTTATCTAGAACTTCAGACTCTGTTGGATTGGGAAGTAGTAACTATGGTTATCCTCATAACCAACCATTTCCCTACCCTTCATATCCCATTCCTGTTGGGATGGAATCGAGCGACTCATGGAATCAATCCCAGCCTCAATCTTCAAATGATTTTTCTTATGGACAACCTCAACCAATCTCGGATCCATATGGGTGGCATATTAACAATTACATGCAAAACTATTTTGGGGATTTATCATTTGATAACTACTCTTCACAATACACTCATTCTACACATAGAGATGATGAAGATAGTGACAAATTTGAACCTCATAGGAACTCTATGTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

390

Amino Acids

43.16

Weight (kDa)

4.75

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimer_Tnp_hAT PF05699 1 - 67 4.9e-13 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 767
AccB7I CCANNNNNTGG 1 cut(s) 764
AccI GTMKAC 1 cut(s) 243
AciI CCGC 1 cut(s) 748
AclWI GGATC 4 cut(s) 29, 439, 1013, 1026
AcsI RAATTY 3 cut(s) 210, 489, 1136
AcuI CTGAAG 2 cut(s) 396, 834
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 5 cut(s) 578, 585, 674, 695, 764
AgsI TTSAA 4 cut(s) 209, 317, 981, 1142
AjnI CCWGG 2 cut(s) 723, 757
AluBI AGCT 6 cut(s) 168, 205, 362, 546, 611, 626
AluI AGCT 6 cut(s) 168, 205, 362, 546, 611, 626
Alw21I GWGCWC 1 cut(s) 119
Alw26I GTCTC 2 cut(s) 9, 558
AlwI GGATC 4 cut(s) 29, 439, 1013, 1026
AlwNI CAGNNNCTG 1 cut(s) 857
ApeKI GCWGC 2 cut(s) 438, 466
ApoI RAATTY 3 cut(s) 210, 489, 1136
AspS9I GGNCC 3 cut(s) 33, 662, 755
AsuC2I CCSGG 1 cut(s) 675
AsuHPI GGTGA 4 cut(s) 263, 293, 561, 710
AsuII TTCGAA 1 cut(s) 453
AvaII GGWCC 3 cut(s) 33, 662, 755
BamHI GGATCC 1 cut(s) 1018
BanI GGYRCC 1 cut(s) 767
BanII GRGCYC 1 cut(s) 689
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 2 cut(s) 450, 478
BccI CCATC 1 cut(s) 931
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 2 cut(s) 725, 759
BcnI CCSGG 1 cut(s) 675
BcoDI GTCTC 2 cut(s) 9, 558
BfaI CTAG 4 cut(s) 260, 435, 549, 843
BisI GCNGC 2 cut(s) 439, 467
BlsI GCNGC 2 cut(s) 440, 468
Bme1390I CCNGG 3 cut(s) 675, 725, 759
Bme18I GGWCC 3 cut(s) 33, 662, 755
BmgT120I GGNCC 3 cut(s) 33, 662, 755
BmiI GGNNCC 3 cut(s) 34, 769, 1020
BmrFI CCNGG 3 cut(s) 675, 725, 759
BmsI GCATC 1 cut(s) 28
BpmI CTGGAG 2 cut(s) 633, 648
Bpu14I TTCGAA 1 cut(s) 453
BpuMI CCSGG 1 cut(s) 675
Bsa29I ATCGAT 1 cut(s) 234
BsaAI YACGTR 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 247, 723, 736
BsaWI WCCGGW 1 cut(s) 578
Bsc4I CCNNNNNNNGG 5 cut(s) 578, 585, 674, 695, 764
Bse1I ACTGG 2 cut(s) 490, 700
BseBI CCWGG 2 cut(s) 725, 759
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 247, 723, 736
BseGI GGATG 3 cut(s) 129, 733, 942
BseLI CCNNNNNNNGG 5 cut(s) 578, 585, 674, 695, 764
BseNI ACTGG 2 cut(s) 490, 700
BseRI GAGGAG 2 cut(s) 434, 437
BseXI GCAGC 2 cut(s) 450, 478
BseYI CCCAGC 2 cut(s) 358, 966
Bsh1285I CGRYCG 1 cut(s) 247
BshNI GGYRCC 1 cut(s) 767
BshVI ATCGAT 1 cut(s) 234
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 2 cut(s) 579, 675
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 5 cut(s) 578, 585, 674, 695, 764
BsmAI GTCTC 2 cut(s) 9, 558
BsmFI GGGAC 1 cut(s) 46
Bsp119I TTCGAA 1 cut(s) 453
Bsp1286I GDGCHC 2 cut(s) 119, 689
Bsp143I GATC 5 cut(s) 21, 235, 431, 557, 1018
Bsp19I CCATGG 1 cut(s) 736
BspACI CCGC 1 cut(s) 748
BspDI ATCGAT 1 cut(s) 234
BspLI GGNNCC 3 cut(s) 34, 769, 1020
BspPI GGATC 4 cut(s) 29, 439, 1013, 1026
BspT104I TTCGAA 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 767
BsrI ACTGG 2 cut(s) 490, 700
BssECI CCNNGG 3 cut(s) 247, 723, 736
BssMI GATC 5 cut(s) 21, 235, 431, 557, 1018
BssT1I CCWWGG 1 cut(s) 736
Bst2UI CCWGG 2 cut(s) 725, 759
Bst4CI ACNGT 2 cut(s) 49, 248
Bst6I CTCTTC 2 cut(s) 336, 1093
BstBAI YACGTR 1 cut(s) 386
BstBI TTCGAA 1 cut(s) 453
BstC8I GCNNGC 2 cut(s) 717, 750
BstDSI CCRYGG 2 cut(s) 247, 736
BstEII GGTNACC 2 cut(s) 251, 302
BstF5I GGATG 3 cut(s) 129, 733, 942
BstKTI GATC 5 cut(s) 24, 238, 434, 560, 1021
BstMAI GTCTC 2 cut(s) 9, 558
BstMBI GATC 5 cut(s) 21, 235, 431, 557, 1018
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 2 cut(s) 725, 759
BstNSI RCATGY 2 cut(s) 719, 1051
BstPI GGTNACC 2 cut(s) 251, 302
BstSCI CCNGG 3 cut(s) 673, 723, 757
BstV1I GCAGC 2 cut(s) 450, 478
BstX2I RGATCY 2 cut(s) 431, 1018
BstXI CCANNNNNNTGG 1 cut(s) 1029
BstYI RGATCY 2 cut(s) 431, 1018
Bsu15I ATCGAT 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 234
BtgI CCRYGG 2 cut(s) 247, 736
BtsCI GGATG 3 cut(s) 129, 733, 942
BtsIMutI CAGTG 3 cut(s) 441, 483, 693
Cac8I GCNNGC 2 cut(s) 717, 750
CaiI CAGNNNCTG 1 cut(s) 857
Cfr13I GGNCC 3 cut(s) 33, 662, 755
ClaI ATCGAT 1 cut(s) 234
Csp6I GTAC 1 cut(s) 552
CviAII CATG 7 cut(s) 25, 199, 239, 716, 737, 954, 1048
CviQI GTAC 1 cut(s) 552
DpnI GATC 5 cut(s) 23, 237, 433, 559, 1020
DpnII GATC 5 cut(s) 21, 235, 431, 557, 1018
Eam1104I CTCTTC 2 cut(s) 336, 1093
EarI CTCTTC 2 cut(s) 336, 1093
Eco130I CCWWGG 1 cut(s) 736
Eco24I GRGCYC 1 cut(s) 689
Eco47I GGWCC 3 cut(s) 33, 662, 755
Eco57I CTGAAG 2 cut(s) 396, 834
Eco91I GGTNACC 2 cut(s) 251, 302
EcoO65I GGTNACC 2 cut(s) 251, 302
EcoRI GAATTC 1 cut(s) 489
EcoRII CCWGG 2 cut(s) 723, 757
EcoT14I CCWWGG 1 cut(s) 736
EcoT38I GRGCYC 1 cut(s) 689
ErhI CCWWGG 1 cut(s) 736
FaeI CATG 7 cut(s) 28, 202, 242, 719, 740, 957, 1051
FaqI GGGAC 1 cut(s) 46
FatI CATG 7 cut(s) 24, 198, 238, 715, 736, 953, 1047
FauI CCCGC 1 cut(s) 741
FauNDI CATATG 1 cut(s) 1024
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 439, 467
FokI GGATG 3 cut(s) 116, 740, 949
FriOI GRGCYC 1 cut(s) 689
Fsp4HI GCNGC 2 cut(s) 439, 467
FspBI CTAG 4 cut(s) 260, 435, 549, 843
GluI GCNGC 2 cut(s) 439, 467
GsaI CCCAGC 2 cut(s) 362, 970
GsuI CTGGAG 2 cut(s) 633, 648
HapII CCGG 2 cut(s) 579, 675
Hin1II CATG 7 cut(s) 28, 202, 242, 719, 740, 957, 1051
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 6 cut(s) 446, 566, 854, 941, 950, 958
HpaII CCGG 2 cut(s) 579, 675
HphI GGTGA 4 cut(s) 263, 293, 561, 710
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 2 cut(s) 853, 1018
Hpy188III TCNNGA 4 cut(s) 215, 561, 824, 843
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 6 cut(s) 344, 386, 590, 700, 772, 924
HpyCH4III ACNGT 2 cut(s) 49, 248
HpyCH4IV ACGT 1 cut(s) 385
HpyCH4V TGCA 5 cut(s) 41, 125, 466, 719, 1051
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpySE526I ACGT 1 cut(s) 385
Hsp92II CATG 7 cut(s) 28, 202, 242, 719, 740, 957, 1051
Kzo9I GATC 5 cut(s) 21, 235, 431, 557, 1018
LmnI GCTCC 4 cut(s) 114, 367, 608, 623
Lsp1109I GCAGC 2 cut(s) 450, 478
LweI GCATC 1 cut(s) 28
MaeI CTAG 4 cut(s) 260, 435, 549, 843
MaeII ACGT 1 cut(s) 385
MaeIII GTNAC 4 cut(s) 251, 302, 875, 1130
MalI GATC 5 cut(s) 23, 237, 433, 559, 1020
MboI GATC 5 cut(s) 21, 235, 431, 557, 1018
MboII GAAGA 8 cut(s) 296, 323, 389, 467, 839, 969, 1080, 1136
MflI RGATCY 2 cut(s) 431, 1018
MhlI GDGCHC 2 cut(s) 119, 689
MluCI AATT 7 cut(s) 109, 176, 210, 489, 762, 1042, 1136
MlyI GAGTC 4 cut(s) 440, 560, 848, 944
MmeI TCCRAC 1 cut(s) 841
MseI TTAA 1 cut(s) 1038
MslI CAYNNNNRTG 3 cut(s) 339, 1027, 1116
MspI CCGG 2 cut(s) 579, 675
MspR9I CCNGG 3 cut(s) 675, 725, 759
MvaI CCWGG 2 cut(s) 725, 759
MwoI GCNNNNNNNGC 1 cut(s) 380
NciI CCSGG 1 cut(s) 675
NcoI CCATGG 1 cut(s) 736
NdeI CATATG 1 cut(s) 1024
NdeII GATC 5 cut(s) 21, 235, 431, 557, 1018
NlaIII CATG 7 cut(s) 28, 202, 242, 719, 740, 957, 1051
NlaIV GGNNCC 3 cut(s) 34, 769, 1020
NmuCI GTSAC 2 cut(s) 251, 1130
NspI RCATGY 2 cut(s) 719, 1051
NspV TTCGAA 1 cut(s) 453
PaeI GCATGC 1 cut(s) 719
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 2 cut(s) 941, 958
PflMI CCANNNNNTGG 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 673
PkrI GCNGC 2 cut(s) 440, 468
PleI GAGTC 4 cut(s) 440, 560, 848, 944
PpsI GAGTC 4 cut(s) 440, 560, 848, 944
Ppu21I YACGTR 1 cut(s) 386
Psp6I CCWGG 2 cut(s) 723, 757
PspEI GGTNACC 2 cut(s) 251, 302
PspFI CCCAGC 2 cut(s) 358, 966
PspGI CCWGG 2 cut(s) 723, 757
PspN4I GGNNCC 3 cut(s) 34, 769, 1020
PspPI GGNCC 3 cut(s) 33, 662, 755
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 2 cut(s) 431, 1018
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
RseI CAYNNNNRTG 3 cut(s) 339, 1027, 1116
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 1038
SatI GCNGC 2 cut(s) 439, 467
Sau3AI GATC 5 cut(s) 21, 235, 431, 557, 1018
Sau96I GGNCC 3 cut(s) 33, 662, 755
SchI GAGTC 4 cut(s) 440, 560, 848, 944
ScrFI CCNGG 3 cut(s) 675, 725, 759
SduI GDGCHC 2 cut(s) 119, 689
SfaNI GCATC 1 cut(s) 28
SfuI TTCGAA 1 cut(s) 453
SinI GGWCC 3 cut(s) 33, 662, 755
SmiMI CAYNNNNRTG 3 cut(s) 339, 1027, 1116
SphI GCATGC 1 cut(s) 719
Sse9I AATT 7 cut(s) 109, 176, 210, 489, 762, 1042, 1136
SsiI CCGC 1 cut(s) 748
SspMI CTAG 4 cut(s) 260, 435, 549, 843
StyD4I CCNGG 3 cut(s) 673, 723, 757
StyI CCWWGG 1 cut(s) 736
TaaI ACNGT 2 cut(s) 49, 248
TaiI ACGT 1 cut(s) 388
TaqI TCGA 5 cut(s) 214, 234, 243, 453, 944
TaqII GACCGA 1 cut(s) 50
TasI AATT 7 cut(s) 109, 176, 210, 489, 762, 1042, 1136
TatI WGTACW 1 cut(s) 551
TfiI GAWTC 2 cut(s) 941, 958
Tru1I TTAA 1 cut(s) 1038
Tru9I TTAA 1 cut(s) 1038
TscAI CASTG 3 cut(s) 448, 490, 700
TseFI GTSAC 2 cut(s) 251, 1130
TseI GCWGC 2 cut(s) 438, 466
Tsp45I GTSAC 2 cut(s) 251, 1130
TspDTI ATGAA 7 cut(s) 215, 323, 363, 405, 609, 906, 1137
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 3 cut(s) 448, 490, 700
Van91I CCANNNNNTGG 1 cut(s) 764
VpaK11BI GGWCC 3 cut(s) 33, 662, 755
XapI RAATTY 3 cut(s) 210, 489, 1136
XbaI TCTAGA 1 cut(s) 842
XceI RCATGY 2 cut(s) 719, 1051
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 4 cut(s) 260, 435, 549, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.