FvH4_2g12511

Protein of unknown function (DUF 659)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000220
Physical Location & Seq
Forward (+)
10973870 .. 10978780
4911 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g12511.t1

Sequence Viewer

Length: 2157 bp
ATGGCTAGTGGGGGAAGTAGACGTGATCCTGCATGGGAGCATGGAGAACAAATTCCTAGCAACAAAAATGGTAGCATATGCAAATATTGTCGGTTGACAATGAAAAGTGGTGGAGTGACACGTCTCAAGTACCACCTAAGCGGCATGGATCCTGGAAACAATGTGTTGCGATGTGAGTCTGTTCCAACAGAAGTGAAGGCATATATTAAATCCTTATTGATGAATAAGCAAAAAGAAAAGGCAAAAAAGGCACATGTGATGGAAGAGGTTCGTGCAGAGCTTCGTGGAGAATTAATTGGCTCACAAGATGACAGTGATGATGAAGGAATTGTATATCCTGATGAGAACATGACCCTTGAGGAACTGAGAGAGTATCGACAAGCAATTCGTAACTCTAAAGAAGCACAATGGAACAGAGAGCACCTCCATAAAATTCCTAACCGGGATGGGTCAAGTAGCGGTACACACATGCGGCGAGCAACAAGTCTACGAGAGCCACAACCAAGTCAACCAACACAACCGTTGGGAGCAAAGTTTAGCAGCTCTTTTCGAGCCCGTCAAAAGAGCATCAAAGATTCCTTCTCAAAGGGTTCAGTGAAGGAGATGATGGGTCGTTTCATCAGTAAGTTTTTCATTTATGATAATATCCCTGCTGAGAAAGCAAATTCTCTTCATTATGAAAATATGGTAATCGGAATCCAAAGAGCAGGAGAAGGAGTACTGCCCCCTACACCTTATGAGATAAGACACAAGTATTTGGATATGGAGTTTAACGACATTACTGAACATGTTAACACACTGAGGGCAACATGGGAGACCTACGGATGCACGATTATGTGCGATGGATGGACAGGGCCAACTAGATTGTCTATCATAAATTTTATGGTTTACTCCAAAGGTAACACTATATTTCTCAAGTCTGTTGATTCTTCTGGTGAGAAGAAAGAGCATGAGTATATATACAAATTGTTGAAAGGAGTAATAAGAGAGGTTGGAGAGCGAAATGTGGTTCAAGTGGTCACTGACAATGCTGCTAATTATGTCAAAGCTGGTAAGAAGTTGATGAAAAACCATAATGTTTATTGGACTTCTTGTGCAGCACACTGCATCGATCTTATGTTCGAAGAAATGGATAAGAAAGAGCAAATTTCTAAGGTGATTGATAAAGACAGAGAGGCAGCTTATTTGCATGTGGACCAGAAAGATCCATTAGATGTCTTTGATATTGGGACAGAGTTTGATGATGAAGCAATAGATCAACTTTGTGAATGGATAAGGCCACTTCATCTTGATGACGAGGAACGCAATCCATCACCTACAGTTGCTCATGAAGCACGTGCGGAAGGAATAAATGTAGAAAGAGTAATTCAAGAAGAGGTTATAGGTCCTGATAGAGAATCTATCGATGCATTAGTGGGTAGAGCTAACATTCTAATTTCCTCTCGTAGCTCACAAGTTCCTTCCAGGACAAATGAAAACTCTAGCACCAGATCAGGAGGCTCATCAAGCATCAGGGGTGATGATAGTGGAAGCTACGGCGGTGGTAGAGATGATGTTGGAGTTCAAGATTACAATGGGAGCCAACCTGGTCATATGAGCCCATTCACTTGTGAGCCAAACTTCACACATGCAACACAAGATGGTGATCATGGAAGTAGGAGAGCAGGTTCTGGTATTGGAGCTATAGGAAAACATTATACACCAAGAGAAAGAGGCCAAGGAAGAATGCCAACGCATGAATATGATTTTTTATCCACTTTTGACTCATCTAGTGTGGGAGGAGGCAATGATGGACCATACACTTATGGCCAAAATCTGACAATGCCTTATTCAACTTATTCCATGCCTTACGGGGTGCAATCAACTGAATTAAGTGATACGGCATGGTCACAGTCTGATGTACAATCTTCCAATGATTATGGTTATGGTCAAGGTCAAGCGCTGCCCAATCCTTACAACTTCCAATACGGAAATCCACAGTCAATCCCTCTTGACCCTTATGGTTGGCATAGTGGGCTAGCATGCGAAGGCGGATTGGAGGATGGTGGCAATCCGACGACGCCGAAGGAGCGGAGCAGGTGGTGGTGGGTTGCTGGCAATAGCATGGAGTTGGTTTGGGCTGCCGGCTCAGGCTGGAGACTGGTCTCTCTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

719

Amino Acids

80.21

Weight (kDa)

5.73

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 243 - 391 7.6e-52 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2067
Acc36I ACCTGC 2 cut(s) 1655, 2067
AccBSI CCGCTC 1 cut(s) 2071
AccI GTMKAC 2 cut(s) 19, 487
AciI CCGC 7 cut(s) 141, 459, 472, 1340, 1539, 2031, 2071
AclWI GGATC 4 cut(s) 20, 143, 156, 1199
AcoI YGGCCR 1 cut(s) 1807
AcsI RAATTY 5 cut(s) 51, 432, 664, 877, 1146
AcvI CACGTG 1 cut(s) 1337
AcyI GRCGYC 1 cut(s) 2060
AfaI GTAC 4 cut(s) 131, 463, 720, 1902
AfeI AGCGCT 1 cut(s) 1941
AflIII ACRYGT 3 cut(s) 119, 253, 787
AgsI TTSAA 5 cut(s) 973, 1013, 1370, 1565, 1833
AjiI CACGTC 2 cut(s) 23, 122
AjnI CCWGG 3 cut(s) 151, 1463, 1585
AloI GAACNNNNNNTCC 2 cut(s) 1651, 1683
AluBI AGCT 8 cut(s) 280, 543, 1049, 1181, 1424, 1449, 1533, 1682
AluI AGCT 8 cut(s) 280, 543, 1049, 1181, 1424, 1449, 1533, 1682
Alw21I GWGCWC 1 cut(s) 423
Alw26I GTCTC 4 cut(s) 128, 809, 2131, 2149
AlwI GGATC 4 cut(s) 20, 143, 156, 1199
AlwNI CAGNNNCTG 1 cut(s) 1670
Aor51HI AGCGCT 1 cut(s) 1941
AoxI GGCC 4 cut(s) 854, 1277, 1714, 1807
ApeKI GCWGC 6 cut(s) 540, 1031, 1097, 1178, 1942, 2120
ApoI RAATTY 5 cut(s) 51, 432, 664, 877, 1146
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
AseI ATTAAT 1 cut(s) 293
Asp700I GAANNNNTTC 2 cut(s) 51, 267
AspLEI GCGC 1 cut(s) 1942
AspS9I GGNCC 4 cut(s) 854, 1195, 1385, 1793
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 5 cut(s) 947, 1168, 1305, 1529, 1655
AsuII TTCGAA 1 cut(s) 1122
AsuNHI GCTAGC 1 cut(s) 2017
AvaII GGWCC 3 cut(s) 1195, 1385, 1793
BalI TGGCCA 1 cut(s) 1809
BamHI GGATCC 1 cut(s) 148
BanII GRGCYC 2 cut(s) 556, 1601
BbrPI CACGTG 1 cut(s) 1337
Bbv12I GWGCWC 1 cut(s) 423
BbvI GCAGC 6 cut(s) 552, 1018, 1109, 1190, 1929, 2107
BccI CCATC 9 cut(s) 253, 440, 601, 836, 840, 1318, 1634, 1784, 2036
BceAI ACGGC 2 cut(s) 1552, 1896
BciT130I CCWGG 3 cut(s) 153, 1465, 1587
BclI TGATCA 1 cut(s) 1645
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 4 cut(s) 128, 809, 2131, 2149
BfaI CTAG 6 cut(s) 6, 57, 861, 1482, 1770, 2018
BfmI CTRYAG 2 cut(s) 1317, 1683
BfoI RGCGCY 1 cut(s) 1943
BfuAI ACCTGC 2 cut(s) 1655, 2067
BisI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1179, 1943, 2121
BlsI GCNGC 8 cut(s) 143, 474, 542, 1033, 1099, 1180, 1944, 2122
BmcAI AGTACT 1 cut(s) 720
Bme1390I CCNGG 4 cut(s) 153, 443, 1465, 1587
Bme18I GGWCC 3 cut(s) 1195, 1385, 1793
BmgBI CACGTC 2 cut(s) 23, 122
BmgT120I GGNCC 4 cut(s) 854, 1195, 1385, 1793
BmiI GGNNCC 2 cut(s) 150, 1580
BmrFI CCNGG 4 cut(s) 153, 443, 1465, 1587
BmsI GCATC 5 cut(s) 576, 815, 1116, 1396, 1518
BmtI GCTAGC 1 cut(s) 2021
BplI GAGNNNNNCTC 2 cut(s) 408, 440
BpmI CTGGAG 1 cut(s) 2155
Bpu10I CCTNAGC 2 cut(s) 137, 2128
Bpu14I TTCGAA 1 cut(s) 1122
BpuEI CTTGAG 3 cut(s) 110, 377, 899
BpuMI CCSGG 1 cut(s) 443
Bsa29I ATCGAT 2 cut(s) 1110, 1404
BsaAI YACGTR 1 cut(s) 1337
BsaBI GATNNNNATC 1 cut(s) 1644
BsaHI GRCGYC 1 cut(s) 2060
BsaI GGTCTC 2 cut(s) 809, 2149
BsaJI CCNNGG 1 cut(s) 1717
BsaXI ACNNNNNCTCC 4 cut(s) 702, 732, 1651, 1681
Bse118I RCCGGY 1 cut(s) 2123
Bse1I ACTGG 1 cut(s) 2145
Bse3DI GCAATG 1 cut(s) 1792
Bse8I GATNNNNATC 1 cut(s) 1644
BseBI CCWGG 3 cut(s) 153, 1465, 1587
BseCI ATCGAT 2 cut(s) 1110, 1404
BseDI CCNNGG 1 cut(s) 1717
BseGI GGATG 4 cut(s) 451, 830, 851, 2047
BseJI GATNNNNATC 1 cut(s) 1644
BseMI GCAATG 1 cut(s) 1792
BseMII CTCAG 4 cut(s) 356, 645, 791, 2142
BseNI ACTGG 1 cut(s) 2145
BseRI GAGGAG 1 cut(s) 1794
BseXI GCAGC 6 cut(s) 552, 1018, 1109, 1190, 1929, 2107
BsgI GTGCAG 2 cut(s) 294, 1116
BshFI GGCC 4 cut(s) 856, 1279, 1716, 1809
BshVI ATCGAT 2 cut(s) 1110, 1404
BsiHKAI GWGCWC 1 cut(s) 423
BsiSI CCGG 2 cut(s) 442, 2124
BslFI GGGAC 1 cut(s) 1243
BsmAI GTCTC 4 cut(s) 128, 809, 2131, 2149
BsmBI CGTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 1243
BsmI GAATGC 1 cut(s) 1731
BsnI GGCC 4 cut(s) 856, 1279, 1716, 1809
Bso31I GGTCTC 2 cut(s) 809, 2149
Bsp119I TTCGAA 1 cut(s) 1122
Bsp1286I GDGCHC 3 cut(s) 423, 556, 1601
Bsp1407I TGTACA 1 cut(s) 1900
Bsp143I GATC 7 cut(s) 25, 148, 1111, 1204, 1255, 1490, 1645
BspACI CCGC 7 cut(s) 141, 459, 472, 1340, 1539, 2031, 2071
BspANI GGCC 4 cut(s) 856, 1279, 1716, 1809
BspCNI CTCAG 4 cut(s) 357, 646, 792, 2141
BspDI ATCGAT 2 cut(s) 1110, 1404
BspHI TCATGA 1 cut(s) 1327
BspLI GGNNCC 2 cut(s) 150, 1580
BspMI ACCTGC 2 cut(s) 1655, 2067
BspOI GCTAGC 1 cut(s) 2021
BspPI GGATC 4 cut(s) 20, 143, 156, 1199
BspT104I TTCGAA 1 cut(s) 1122
BspTNI GGTCTC 2 cut(s) 809, 2149
BsrBI CCGCTC 1 cut(s) 2071
BsrDI GCAATG 1 cut(s) 1792
BsrFI RCCGGY 1 cut(s) 2123
BsrGI TGTACA 1 cut(s) 1900
BsrI ACTGG 1 cut(s) 2145
BssAI RCCGGY 1 cut(s) 2123
BssECI CCNNGG 1 cut(s) 1717
BssMI GATC 7 cut(s) 25, 148, 1111, 1204, 1255, 1490, 1645
BssNI GRCGYC 1 cut(s) 2060
BssT1I CCWWGG 1 cut(s) 1717
Bst2UI CCWGG 3 cut(s) 153, 1465, 1587
Bst4CI ACNGT 5 cut(s) 314, 522, 1321, 1893, 1980
Bst6I CTCTTC 3 cut(s) 258, 675, 1368
BstACI GRCGYC 1 cut(s) 2060
BstAUI TGTACA 1 cut(s) 1900
BstBAI YACGTR 1 cut(s) 1337
BstBI TTCGAA 1 cut(s) 1122
BstC8I GCNNGC 5 cut(s) 477, 2019, 2023, 2095, 2125
BstDEI CTNAG 6 cut(s) 137, 365, 654, 800, 1152, 2128
BstF5I GGATG 4 cut(s) 451, 830, 851, 2047
BstH2I RGCGCY 1 cut(s) 1943
BstHHI GCGC 1 cut(s) 1942
BstKTI GATC 7 cut(s) 28, 151, 1114, 1207, 1258, 1493, 1648
BstMAI GTCTC 4 cut(s) 128, 809, 2131, 2149
BstMBI GATC 7 cut(s) 25, 148, 1111, 1204, 1255, 1490, 1645
BstMWI GCNNNNNNNGC 6 cut(s) 248, 659, 1331, 1506, 2014, 2068
BstNI CCWGG 3 cut(s) 153, 1465, 1587
BstNSI RCATGY 6 cut(s) 257, 472, 791, 1193, 1631, 2025
BstSCI CCNGG 4 cut(s) 151, 441, 1463, 1585
BstSFI CTRYAG 2 cut(s) 1317, 1683
BstV1I GCAGC 6 cut(s) 552, 1018, 1109, 1190, 1929, 2107
BstX2I RGATCY 2 cut(s) 148, 1204
BstYI RGATCY 2 cut(s) 148, 1204
Bsu15I ATCGAT 2 cut(s) 1110, 1404
BsuRI GGCC 4 cut(s) 856, 1279, 1716, 1809
BsuTUI ATCGAT 2 cut(s) 1110, 1404
BtgZI GCGATG 2 cut(s) 184, 855
BtrI CACGTC 2 cut(s) 23, 122
BtsCI GGATG 4 cut(s) 451, 830, 851, 2047
BtsI GCAGTG 1 cut(s) 1102
BtsIMutI CAGTG 5 cut(s) 319, 600, 797, 1020, 1102
BveI ACCTGC 2 cut(s) 1655, 2067
Cac8I GCNNGC 5 cut(s) 477, 2019, 2023, 2095, 2125
CaiI CAGNNNCTG 1 cut(s) 1670
CciI TCATGA 1 cut(s) 1327
CfoI GCGC 1 cut(s) 1942
Cfr10I RCCGGY 1 cut(s) 2123
Cfr13I GGNCC 4 cut(s) 854, 1195, 1385, 1793
ClaI ATCGAT 2 cut(s) 1110, 1404
CseI GACGC 1 cut(s) 2068
CsiI ACCWGGT 1 cut(s) 1585
Csp6I GTAC 4 cut(s) 130, 462, 719, 1901
CviQI GTAC 4 cut(s) 130, 462, 719, 1901
DdeI CTNAG 6 cut(s) 137, 365, 654, 800, 1152, 2128
DpnI GATC 7 cut(s) 27, 150, 1113, 1206, 1257, 1492, 1647
DpnII GATC 7 cut(s) 25, 148, 1111, 1204, 1255, 1490, 1645
EaeI YGGCCR 1 cut(s) 1807
Eam1104I CTCTTC 3 cut(s) 258, 675, 1368
EarI CTCTTC 3 cut(s) 258, 675, 1368
EciI GGCGGA 1 cut(s) 2046
Eco130I CCWWGG 1 cut(s) 1717
Eco24I GRGCYC 2 cut(s) 556, 1601
Eco31I GGTCTC 2 cut(s) 809, 2149
Eco47I GGWCC 3 cut(s) 1195, 1385, 1793
Eco47III AGCGCT 1 cut(s) 1941
Eco72I CACGTG 1 cut(s) 1337
EcoO109I RGGNCCY 1 cut(s) 1385
EcoRII CCWGG 3 cut(s) 151, 1463, 1585
EcoT14I CCWWGG 1 cut(s) 1717
EcoT22I ATGCAT 1 cut(s) 1411
EcoT38I GRGCYC 2 cut(s) 556, 1601
ErhI CCWWGG 1 cut(s) 1717
Esp3I CGTCTC 1 cut(s) 128
FaqI GGGAC 1 cut(s) 1243
FauNDI CATATG 2 cut(s) 77, 1593
FbaI TGATCA 1 cut(s) 1645
FblI GTMKAC 2 cut(s) 19, 487
Fnu4HI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1179, 1943, 2121
FokI GGATG 4 cut(s) 458, 837, 858, 2054
FriOI GRGCYC 2 cut(s) 556, 1601
Fsp4HI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1179, 1943, 2121
FspBI CTAG 6 cut(s) 6, 57, 861, 1482, 1770, 2018
GlaI GCGC 1 cut(s) 1941
GluI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1179, 1943, 2121
GsuI CTGGAG 1 cut(s) 2155
HaeII RGCGCY 1 cut(s) 1943
HaeIII GGCC 4 cut(s) 856, 1279, 1716, 1809
HapII CCGG 2 cut(s) 442, 2124
HgaI GACGC 1 cut(s) 2068
HhaI GCGC 1 cut(s) 1942
Hin1I GRCGYC 1 cut(s) 2060
Hin6I GCGC 1 cut(s) 1940
HinP1I GCGC 1 cut(s) 1940
HincII GTYRAC 3 cut(s) 96, 509, 793
HindII GTYRAC 3 cut(s) 96, 509, 793
HinfI GANTC 6 cut(s) 176, 575, 696, 926, 1397, 1763
HpaI GTTAAC 1 cut(s) 793
HpaII CCGG 2 cut(s) 442, 2124
HphI GGTGA 5 cut(s) 947, 1168, 1305, 1529, 1655
Hpy166II GTNNAC 8 cut(s) 20, 96, 464, 488, 509, 793, 889, 1195
Hpy188I TCNGA 4 cut(s) 695, 1818, 1897, 2055
Hpy188III TCNNGA 9 cut(s) 338, 1289, 1328, 1370, 1388, 1494, 1565, 1991, 2154
Hpy8I GTNNAC 8 cut(s) 20, 96, 464, 488, 509, 793, 889, 1195
Hpy99I CGWCG 2 cut(s) 2059, 2062
HpyAV CCTTC 9 cut(s) 190, 317, 589, 592, 707, 1337, 1470, 2021, 2059
HpyCH4III ACNGT 5 cut(s) 314, 522, 1321, 1893, 1980
HpyCH4IV ACGT 3 cut(s) 22, 121, 1336
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 659, 1331, 1506, 2014, 2068
HpyF3I CTNAG 6 cut(s) 137, 365, 654, 800, 1152, 2128
HpySE526I ACGT 3 cut(s) 22, 121, 1336
Hsp92I GRCGYC 1 cut(s) 2060
HspAI GCGC 1 cut(s) 1940
KroI GCCGGC 1 cut(s) 2123
KroNI GCCGGC 1 cut(s) 2125
Ksp22I TGATCA 1 cut(s) 1645
KspAI GTTAAC 1 cut(s) 793
Kzo9I GATC 7 cut(s) 25, 148, 1111, 1204, 1255, 1490, 1645
LmnI GCTCC 6 cut(s) 37, 527, 1578, 1679, 2068, 2073
Lsp1109I GCAGC 6 cut(s) 552, 1018, 1109, 1190, 1929, 2107
LweI GCATC 5 cut(s) 576, 815, 1116, 1396, 1518
MabI ACCWGGT 1 cut(s) 1585
MaeI CTAG 6 cut(s) 6, 57, 861, 1482, 1770, 2018
MaeII ACGT 3 cut(s) 22, 121, 1336
MaeIII GTNAC 5 cut(s) 115, 389, 899, 1018, 1887
MalI GATC 7 cut(s) 27, 150, 1113, 1206, 1257, 1492, 1647
MbiI CCGCTC 1 cut(s) 2071
MboI GATC 7 cut(s) 25, 148, 1111, 1204, 1255, 1490, 1645
MboII GAAGA 8 cut(s) 275, 662, 921, 952, 1136, 1385, 1734, 1899
MflI RGATCY 2 cut(s) 148, 1204
MhlI GDGCHC 3 cut(s) 423, 556, 1601
MlsI TGGCCA 1 cut(s) 1809
MluNI TGGCCA 1 cut(s) 1809
MlyI GAGTC 2 cut(s) 185, 1757
MmeI TCCRAC 4 cut(s) 209, 973, 1537, 2078
Mox20I TGGCCA 1 cut(s) 1809
Mph1103I ATGCAT 1 cut(s) 1411
MroNI GCCGGC 1 cut(s) 2123
MroXI GAANNNNTTC 2 cut(s) 51, 267
MscI TGGCCA 1 cut(s) 1809
MseI TTAA 5 cut(s) 207, 293, 771, 792, 1871
MslI CAYNNNNRTG 3 cut(s) 833, 1290, 1740
Msp20I TGGCCA 1 cut(s) 1809
MspI CCGG 2 cut(s) 442, 2124
MspR9I CCNGG 4 cut(s) 153, 443, 1465, 1587
Mva1269I GAATGC 1 cut(s) 1731
MvaI CCWGG 3 cut(s) 153, 1465, 1587
MwoI GCNNNNNNNGC 6 cut(s) 248, 659, 1331, 1506, 2014, 2068
NaeI GCCGGC 1 cut(s) 2125
NciI CCSGG 1 cut(s) 443
NdeI CATATG 2 cut(s) 77, 1593
NdeII GATC 7 cut(s) 25, 148, 1111, 1204, 1255, 1490, 1645
NgoMIV GCCGGC 1 cut(s) 2123
NheI GCTAGC 1 cut(s) 2017
NlaIV GGNNCC 2 cut(s) 150, 1580
NmuCI GTSAC 3 cut(s) 115, 1018, 1887
NsiI ATGCAT 1 cut(s) 1411
NspI RCATGY 6 cut(s) 257, 472, 791, 1193, 1631, 2025
NspV TTCGAA 1 cut(s) 1122
PaeI GCATGC 1 cut(s) 2025
PagI TCATGA 1 cut(s) 1327
PaqCI CACCTGC 1 cut(s) 2067
PciI ACATGT 2 cut(s) 253, 787
PctI GAATGC 1 cut(s) 1731
PdiI GCCGGC 1 cut(s) 2125
PdmI GAANNNNTTC 2 cut(s) 51, 267
PfeI GAWTC 4 cut(s) 575, 696, 926, 1397
PfoI TCCNGGA 2 cut(s) 151, 1463
PkrI GCNGC 8 cut(s) 143, 474, 542, 1033, 1099, 1180, 1944, 2122
PleI GAGTC 2 cut(s) 184, 1757
PmaCI CACGTG 1 cut(s) 1337
PmlI CACGTG 1 cut(s) 1337
PpsI GAGTC 2 cut(s) 184, 1757
Ppu21I YACGTR 1 cut(s) 1337
PpuMI RGGWCCY 1 cut(s) 1385
PscI ACATGT 2 cut(s) 253, 787
PshBI ATTAAT 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 1385
Psp6I CCWGG 3 cut(s) 151, 1463, 1585
PspCI CACGTG 1 cut(s) 1337
PspGI CCWGG 3 cut(s) 151, 1463, 1585
PspN4I GGNNCC 2 cut(s) 150, 1580
PspPI GGNCC 4 cut(s) 854, 1195, 1385, 1793
PspPPI RGGWCCY 1 cut(s) 1385
PstNI CAGNNNCTG 1 cut(s) 1670
PsuI RGATCY 2 cut(s) 148, 1204
RsaI GTAC 4 cut(s) 131, 463, 720, 1902
RsaNI GTAC 4 cut(s) 130, 462, 719, 1901
RseI CAYNNNNRTG 3 cut(s) 833, 1290, 1740
SaqAI TTAA 5 cut(s) 207, 293, 771, 792, 1871
SatI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1179, 1943, 2121
Sau3AI GATC 7 cut(s) 25, 148, 1111, 1204, 1255, 1490, 1645
Sau96I GGNCC 4 cut(s) 854, 1195, 1385, 1793
ScaI AGTACT 1 cut(s) 720
SchI GAGTC 2 cut(s) 185, 1757
ScrFI CCNGG 4 cut(s) 153, 443, 1465, 1587
SduI GDGCHC 3 cut(s) 423, 556, 1601
SexAI ACCWGGT 1 cut(s) 1585
SfaNI GCATC 5 cut(s) 576, 815, 1116, 1396, 1518
SfcI CTRYAG 2 cut(s) 1317, 1683
SfuI TTCGAA 1 cut(s) 1122
SinI GGWCC 3 cut(s) 1195, 1385, 1793
SmiMI CAYNNNNRTG 3 cut(s) 833, 1290, 1740
SmlI CTYRAG 3 cut(s) 125, 356, 914
SmoI CTYRAG 3 cut(s) 125, 356, 914
SphI GCATGC 1 cut(s) 2025
SsiI CCGC 7 cut(s) 141, 459, 472, 1340, 1539, 2031, 2071
SspI AATATT 1 cut(s) 86
SspMI CTAG 6 cut(s) 6, 57, 861, 1482, 1770, 2018
StyD4I CCNGG 4 cut(s) 151, 441, 1463, 1585
StyI CCWWGG 1 cut(s) 1717
TaaI ACNGT 5 cut(s) 314, 522, 1321, 1893, 1980
TaiI ACGT 3 cut(s) 25, 124, 1339
TaqI TCGA 5 cut(s) 376, 550, 1110, 1122, 1404
TatI WGTACW 2 cut(s) 718, 1900
TauI GCSGC 2 cut(s) 144, 475
TfiI GAWTC 4 cut(s) 575, 696, 926, 1397
Tru1I TTAA 5 cut(s) 207, 293, 771, 792, 1871
Tru9I TTAA 5 cut(s) 207, 293, 771, 792, 1871
TscAI CASTG 5 cut(s) 319, 600, 804, 1027, 1109
TseFI GTSAC 3 cut(s) 115, 1018, 1887
TseI GCWGC 6 cut(s) 540, 1031, 1097, 1178, 1942, 2120
Tsp45I GTSAC 3 cut(s) 115, 1018, 1887
TspGWI ACGGA 2 cut(s) 837, 1983
TspRI CASTG 5 cut(s) 319, 600, 804, 1027, 1109
VpaK11BI GGWCC 3 cut(s) 1195, 1385, 1793
VspI ATTAAT 1 cut(s) 293
XapI RAATTY 5 cut(s) 51, 432, 664, 877, 1146
XceI RCATGY 6 cut(s) 257, 472, 791, 1193, 1631, 2025
XmiI GTMKAC 2 cut(s) 19, 487
XmnI GAANNNNTTC 2 cut(s) 51, 267
XspI CTAG 6 cut(s) 6, 57, 861, 1482, 1770, 2018
ZrmI AGTACT 1 cut(s) 720
Zsp2I ATGCAT 1 cut(s) 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.