FvH4_6g35011

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000220
Physical Location & Seq
Forward (+)
27667900 .. 27671399
3500 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35011.t1

Sequence Viewer

Length: 3024 bp
ATGGCTAGTGGGGGAAGTAGACGTGATCCTGCATGGGAGCATGGAGAACAAATTCCTAGCAACAAAAATGGTAGCATATGCAAATATTGTCGGTTGACAATGAAAAGTGGTGGAGTGACACGTCTCAAGTACCACCTAAGCGGCATGGATCCTGGAAACAATGTGTTGCGATGTGAGTCTGTTCCAACAGAAGTGAAGGCATATATTAAATCCTTATTGATGAATAAGCAAAAAGAAAAGGCAAAAAAGGCACATGTGATGGAAGAGGTTCGTGCAAAGCTTCGTGGAGAATTAATTGGCTCACAAGATGACAGTGATGATGAAGGAATTGTATATCCTGATGAGAACATGACCCTTGAGGAACTGAGAGAGTATCGACAAGCAATTCGTAACTCTAAAGAAGCACAATGGAACAGAGAGCACCTCCATAAAGTACCTAACCGGGATGGGTCAAGTAGCGGTACACACATGCGGCGAGCAACAAGTCTACGAGAGCCACAACCAAGTCAACCAACACAACCGTTGGGAGCAAAGTTTAGCAGCTCTTTTCGAGCCCGTCAAAAGAGCATCAAAGATTCCTTCTCAAATGGTTCAATGAAGGAGATGATGGGTCGTTTCATCAGTAAGTTTTTCATTTATGATAATATCCTTGCTGAGAAAGCAAATTCTCTTCATTATGAAAATATGGTAATCGGAATCCAAAGAGCAGGAGAAGGAGTACTGCCCCCTACACCTTATGAGATAAGACACAAGTATTTGGATATGGAGTTTAACGACATTACTGAACATGTTAACACACTGAGGGCAACATGGGAGACCTACGAATGCACGATTATGTGTGATGGATGGACATGGCCAACTAGATTGTCTATCATAAATTTTATGGTTTACTCCAAAGGTAACACTATATTTCTCAAGTCTGTTGATTCTTCTGGTGAGAAGAAAGAGCATGACTATATATACAAATTGTTGAAAGGAGTAATAAGAGAGGTTGGAGAGCGAAATGTGGTTCAAGTGGTCACTGACAATGCTGCTAATTATGTCAAAGCTGGTAAGAAGTTGATGAAAAACCATAATGTTTATTGGACTTCTTGTGCAGCACACTGCATCGATCTTATGTTCGAAGAAATGGGTAAGAAAGAGCAAATTTCTAAGGTGATTGATAAAGGTAAAATTATTTCAAAGTACATTTACAATCACAATTTGTTGTTGACCAAGATGCGAGAGTTTTGTAAAGGAGAAATTATTCGTGCCGCACCAACTCGATTCGCAACAAATTTCATTTCCTTGGAAAGCTTGATCAAGAAAAAGGCGGGATTGAAGCAGTTATTTTCCAGTGATTGGTGGGAGAGTAGCAATTTTCGTCGCACCAAACATGCGCGTGTCGTTGAAAATATTGTATTTGATGCACAATTTTGGAGTCAGGCCGAACATGTTTGTCAAATATCTGAGCCCTTTTATAAGGTATTACGCATCGTAGACACTGAAGTATATCCAACCATGGGTGTCGTTTATGAGCTAATGCGTGTAGTTAGACAAGAGTTGAACCAAAAACAAGGTGCAAAGTGGGTCGTGAAAATTATCGATGATCGGTGGTTTAAAACTCTATGGCATGATTTGCATTCGGCAGGTTATTATTTAAACCCTCGATACAACTATAAATCTGGCGTGGGAGACGATTATAAGCTGATTGAAGCTGTGCATAAAGTTTTTTCAAGATGTGACCCTAACTGTCCAACAATAGGCCAATTTGGAAAGGAGCTTACATATTTTAAAGATGCAAAGTTAAACTTTGGAATGCCGGCAGCCATTGCCTCAAGATCAGAGATGTCTCCAACCGACTGGTGGGTTATGTATGGGAGTAGTGCCCCAACTGTGAGAACGTTAGCTATCAAAGTATTATCACAAACAGCATCAGCATCTGCGTGTGAGAGAAACTGGAGCACATTCGTATTAATCCATACTAAGCAGAGAAACAGGTTGGGGTATAGTCGTTTGGAAAAATTGGTTTTTTGCTATTACAACATGAGGCTTAAAATTCGAGACAGAGAGACAGCTTATTCGGATGTGGACCAGAAAGATCCATTAGATGTCTTTGATATTGGTACAGAGTTTGATGATGAAGCAATAGATCAACTTTGTGAATGGATAAGGCCACTTCATCTTGATGACGAGGAACGCAATCCATCACCTACAGTTGCTCGTGAAGCACGTGCGGAAGGAATAAATGTAGAAAGAGTAATTCAAGAAGAGGTTATAGGTCCTGATAGAGAATCTATCGATGCATTAGTGGGTAGAGCTAACATTCTAATTTCCTCTCGTAGCTCACAAGTTCCTTCCAGGACAAACGAAAACTCTAGCACCAGATCAGGAGGCTCATCAAGCATCAGGGGTGATGATAGTGGAAGCTACGGCGGTGGTGGAGATGATGTTGGAGTTCAAGATTACAATGGGAGCCAACCTGGTCATATGAGCCCATTCACTTGTGAGCCAAACTTCACACATGCAACACAAGATGGTGATCATGGAAGTAGGAGAGCAGGTTCTGGTATTGGAGCTATAGGAAAACATTATACACCAAGAGAAAGAGGCCAAGGAAGAATGCCAACGCATAAATATGATTTTTTATCCACTTTTGACTCATCTAGTGTGGGAGGAGGCAATGATGGACCATACACTTATGGCCAAAATCTGACAATGCCTTATTCAACTTATTCCATGCCTTACAGGGTGCAATCAACTGAATCAAGTGATACGGCATGGTCACAGTCTGATGTACAATCTTCCAATGATTATGGTTATGGTCAAGGTCAAGCGCTGCCCAATCCTTACAACTTCCAATACGGAAATCCACAGTCAATCCCTCTTGACCCTTATGGTTGGCATGTAAGTCAATATATGCAAAACTACAAAGGTGAGGTATCATTTAATGACTACTCTTCACAATATACTGAAGACTCTACACAAAGAAACGATGAAGATAGTGAAAATTATGTACCTCATAGGTCTTCTATGTGGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

1008

Amino Acids

114.7

Weight (kDa)

7.34

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 243 - 393 1.4e-51 Domain of unknown function (DUF659)
Dimer_Tnp_hAT PF05699 607 - 676 1e-12 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1461, 1683
Acc36I ACCTGC 2 cut(s) 1619, 2531
AccB7I CCANNNNNTGG 1 cut(s) 1341
AccI GTMKAC 3 cut(s) 19, 487, 1479
AccII CGCG 1 cut(s) 1381
AciI CCGC 7 cut(s) 141, 459, 472, 1254, 1313, 2216, 2415
AclI AACGTT 1 cut(s) 1883
AclWI GGATC 4 cut(s) 20, 143, 156, 2075
AcoI YGGCCR 2 cut(s) 854, 2683
AcsI RAATTY 6 cut(s) 51, 664, 877, 1146, 1276, 2037
AcuI CTGAAG 2 cut(s) 1506, 2973
AcvI CACGTG 1 cut(s) 2213
AfaI GTAC 8 cut(s) 131, 435, 463, 720, 1187, 2107, 2778, 2997
AfeI AGCGCT 1 cut(s) 2817
AfiI CCNNNNNNNGG 4 cut(s) 1341, 1502, 1742, 1845
AflIII ACRYGT 4 cut(s) 119, 253, 787, 1432
AjiI CACGTC 2 cut(s) 23, 122
AjnI CCWGG 3 cut(s) 151, 2339, 2461
AloI GAACNNNNNNTCC 2 cut(s) 2527, 2559
Alw21I GWGCWC 2 cut(s) 423, 1946
Alw26I GTCTC 6 cut(s) 128, 809, 1668, 1836, 2037, 2045
AlwI GGATC 4 cut(s) 20, 143, 156, 2075
AlwNI CAGNNNCTG 2 cut(s) 1922, 2546
Aor51HI AGCGCT 1 cut(s) 2817
AoxI GGCC 6 cut(s) 854, 1425, 1744, 2153, 2590, 2683
ApeKI GCWGC 5 cut(s) 540, 1031, 1097, 1805, 2818
ApoI RAATTY 6 cut(s) 51, 664, 877, 1146, 1276, 2037
ArsI GACNNNNNNTTYG 4 cut(s) 369, 401, 2044, 2076
AseI ATTAAT 2 cut(s) 293, 1955
Asp700I GAANNNNTTC 3 cut(s) 51, 267, 1245
AspLEI GCGC 2 cut(s) 1381, 2818
AspS9I GGNCC 3 cut(s) 2071, 2261, 2669
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 6 cut(s) 947, 1168, 2181, 2405, 2531, 2927
AsuII TTCGAA 1 cut(s) 1122
AvaII GGWCC 3 cut(s) 2071, 2261, 2669
BaeGI GKGCMC 1 cut(s) 1870
BalI TGGCCA 2 cut(s) 856, 2685
BamHI GGATCC 1 cut(s) 148
BanII GRGCYC 3 cut(s) 556, 1455, 2477
BauI CACGAG 1 cut(s) 2202
BbrPI CACGTG 1 cut(s) 2213
BbsI GAAGAC 2 cut(s) 2961, 3000
Bbv12I GWGCWC 2 cut(s) 423, 1946
BbvI GCAGC 5 cut(s) 552, 1018, 1109, 1817, 2805
BccI CCATC 8 cut(s) 253, 440, 601, 836, 840, 2194, 2510, 2660
BceAI ACGGC 2 cut(s) 2428, 2772
BciT130I CCWGG 3 cut(s) 153, 2341, 2463
BclI TGATCA 2 cut(s) 1299, 2521
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 6 cut(s) 128, 809, 1668, 1836, 2037, 2045
BfaI CTAG 5 cut(s) 6, 57, 861, 2358, 2646
BfmI CTRYAG 2 cut(s) 2193, 2559
BfoI RGCGCY 1 cut(s) 2819
BfuAI ACCTGC 2 cut(s) 1619, 2531
BisI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1254, 1806, 2819
BlsI GCNGC 8 cut(s) 143, 474, 542, 1033, 1099, 1255, 1807, 2820
BmcAI AGTACT 1 cut(s) 720
Bme1390I CCNGG 4 cut(s) 153, 443, 2341, 2463
Bme18I GGWCC 3 cut(s) 2071, 2261, 2669
BmgBI CACGTC 2 cut(s) 23, 122
BmgT120I GGNCC 3 cut(s) 2071, 2261, 2669
BmiI GGNNCC 2 cut(s) 150, 2456
BmrFI CCNGG 4 cut(s) 153, 443, 2341, 2463
BpiI GAAGAC 2 cut(s) 2961, 3000
BplI GAGNNNNNCTC 2 cut(s) 408, 440
BpmI CTGGAG 1 cut(s) 1960
Bpu10I CCTNAGC 1 cut(s) 137
Bpu14I TTCGAA 1 cut(s) 1122
BpuEI CTTGAG 4 cut(s) 110, 377, 899, 1801
BpuMI CCSGG 1 cut(s) 443
Bsa29I ATCGAT 3 cut(s) 1110, 1584, 2280
BsaAI YACGTR 1 cut(s) 2213
BsaBI GATNNNNATC 1 cut(s) 2520
BsaI GGTCTC 1 cut(s) 809
BsaJI CCNNGG 3 cut(s) 1287, 1500, 2593
BsaXI ACNNNNNCTCC 6 cut(s) 702, 732, 2415, 2445, 2527, 2557
Bsc4I CCNNNNNNNGG 4 cut(s) 1341, 1502, 1742, 1845
Bse118I RCCGGY 1 cut(s) 1801
Bse1I ACTGG 3 cut(s) 1335, 1847, 1943
Bse3DI GCAATG 2 cut(s) 1809, 2668
Bse8I GATNNNNATC 1 cut(s) 2520
BseBI CCWGG 3 cut(s) 153, 2341, 2463
BseCI ATCGAT 3 cut(s) 1110, 1584, 2280
BseDI CCNNGG 3 cut(s) 1287, 1500, 2593
BseGI GGATG 3 cut(s) 451, 851, 2071
BseJI GATNNNNATC 1 cut(s) 2520
BseLI CCNNNNNNNGG 4 cut(s) 1341, 1502, 1742, 1845
BseMI GCAATG 2 cut(s) 1809, 2668
BseMII CTCAG 4 cut(s) 356, 645, 791, 1440
BseNI ACTGG 3 cut(s) 1335, 1847, 1943
BseRI GAGGAG 1 cut(s) 2670
BseSI GKGCMC 1 cut(s) 1870
BseXI GCAGC 5 cut(s) 552, 1018, 1109, 1817, 2805
BsgI GTGCAG 1 cut(s) 1116
Bsh1236I CGCG 1 cut(s) 1381
BshFI GGCC 6 cut(s) 856, 1427, 1746, 2155, 2592, 2685
BshVI ATCGAT 3 cut(s) 1110, 1584, 2280
BsiHKAI GWGCWC 2 cut(s) 423, 1946
BsiSI CCGG 2 cut(s) 442, 1802
BslI CCNNNNNNNGG 4 cut(s) 1341, 1502, 1742, 1845
BsmAI GTCTC 6 cut(s) 128, 809, 1668, 1836, 2037, 2045
BsmBI CGTCTC 2 cut(s) 128, 1668
BsmI GAATGC 4 cut(s) 830, 1621, 1803, 2607
BsnI GGCC 6 cut(s) 856, 1427, 1746, 2155, 2592, 2685
Bso31I GGTCTC 1 cut(s) 809
Bsp119I TTCGAA 1 cut(s) 1122
Bsp1286I GDGCHC 6 cut(s) 423, 556, 1455, 1870, 1946, 2477
Bsp1407I TGTACA 1 cut(s) 2776
Bsp19I CCATGG 1 cut(s) 1500
BspACI CCGC 7 cut(s) 141, 459, 472, 1254, 1313, 2216, 2415
BspANI GGCC 6 cut(s) 856, 1427, 1746, 2155, 2592, 2685
BspCNI CTCAG 4 cut(s) 357, 646, 792, 1441
BspDI ATCGAT 3 cut(s) 1110, 1584, 2280
BspFNI CGCG 1 cut(s) 1381
BspLI GGNNCC 2 cut(s) 150, 2456
BspMI ACCTGC 2 cut(s) 1619, 2531
BspPI GGATC 4 cut(s) 20, 143, 156, 2075
BspT104I TTCGAA 1 cut(s) 1122
BspTNI GGTCTC 1 cut(s) 809
BsrDI GCAATG 2 cut(s) 1809, 2668
BsrFI RCCGGY 1 cut(s) 1801
BsrGI TGTACA 1 cut(s) 2776
BsrI ACTGG 3 cut(s) 1335, 1847, 1943
BssAI RCCGGY 1 cut(s) 1801
BssECI CCNNGG 3 cut(s) 1287, 1500, 2593
BssSI CACGAG 1 cut(s) 2202
BssT1I CCWWGG 3 cut(s) 1287, 1500, 2593
Bst2BI CACGAG 1 cut(s) 2202
Bst2UI CCWGG 3 cut(s) 153, 2341, 2463
Bst4CI ACNGT 7 cut(s) 314, 522, 1733, 1876, 2197, 2769, 2856
Bst6I CTCTTC 4 cut(s) 258, 675, 2244, 2944
BstAPI GCANNNNNTGC 2 cut(s) 1618, 1811
BstAUI TGTACA 1 cut(s) 2776
BstBAI YACGTR 1 cut(s) 2213
BstBI TTCGAA 1 cut(s) 1122
BstC8I GCNNGC 2 cut(s) 477, 1803
BstDEI CTNAG 7 cut(s) 137, 365, 654, 800, 1152, 1449, 1965
BstDSI CCRYGG 1 cut(s) 1500
BstF5I GGATG 3 cut(s) 451, 851, 2071
BstFNI CGCG 1 cut(s) 1381
BstH2I RGCGCY 1 cut(s) 2819
BstHHI GCGC 2 cut(s) 1381, 2818
BstMAI GTCTC 6 cut(s) 128, 809, 1668, 1836, 2037, 2045
BstMWI GCNNNNNNNGC 6 cut(s) 248, 659, 1618, 1811, 2207, 2382
BstNI CCWGG 3 cut(s) 153, 2341, 2463
BstNSI RCATGY 7 cut(s) 257, 472, 791, 1379, 1436, 2507, 2888
BstSCI CCNGG 4 cut(s) 151, 441, 2339, 2461
BstSFI CTRYAG 2 cut(s) 2193, 2559
BstSLI GKGCMC 1 cut(s) 1870
BstUI CGCG 1 cut(s) 1381
BstV1I GCAGC 5 cut(s) 552, 1018, 1109, 1817, 2805
BstV2I GAAGAC 2 cut(s) 2961, 3000
BstX2I RGATCY 2 cut(s) 148, 2080
BstXI CCANNNNNNTGG 1 cut(s) 1842
BstYI RGATCY 2 cut(s) 148, 2080
Bsu15I ATCGAT 3 cut(s) 1110, 1584, 2280
BsuRI GGCC 6 cut(s) 856, 1427, 1746, 2155, 2592, 2685
BsuTUI ATCGAT 3 cut(s) 1110, 1584, 2280
BtgI CCRYGG 1 cut(s) 1500
BtgZI GCGATG 1 cut(s) 184
BtrI CACGTC 2 cut(s) 23, 122
BtsCI GGATG 3 cut(s) 451, 851, 2071
BtsI GCAGTG 1 cut(s) 1102
BtsIMutI CAGTG 6 cut(s) 319, 797, 1020, 1102, 1342, 1482
BveI ACCTGC 2 cut(s) 1619, 2531
Cac8I GCNNGC 2 cut(s) 477, 1803
CaiI CAGNNNCTG 2 cut(s) 1922, 2546
CfoI GCGC 2 cut(s) 1381, 2818
Cfr10I RCCGGY 1 cut(s) 1801
Cfr13I GGNCC 3 cut(s) 2071, 2261, 2669
ClaI ATCGAT 3 cut(s) 1110, 1584, 2280
CsiI ACCWGGT 1 cut(s) 2461
Csp6I GTAC 8 cut(s) 130, 434, 462, 719, 1186, 2106, 2777, 2996
CviQI GTAC 8 cut(s) 130, 434, 462, 719, 1186, 2106, 2777, 2996
DdeI CTNAG 7 cut(s) 137, 365, 654, 800, 1152, 1449, 1965
DraI TTTAAA 3 cut(s) 1600, 1641, 1774
EaeI YGGCCR 2 cut(s) 854, 2683
Eam1104I CTCTTC 4 cut(s) 258, 675, 2244, 2944
EarI CTCTTC 4 cut(s) 258, 675, 2244, 2944
Eco130I CCWWGG 3 cut(s) 1287, 1500, 2593
Eco24I GRGCYC 3 cut(s) 556, 1455, 2477
Eco31I GGTCTC 1 cut(s) 809
Eco47I GGWCC 3 cut(s) 2071, 2261, 2669
Eco47III AGCGCT 1 cut(s) 2817
Eco57I CTGAAG 2 cut(s) 1506, 2973
Eco72I CACGTG 1 cut(s) 2213
EcoO109I RGGNCCY 1 cut(s) 2261
EcoRII CCWGG 3 cut(s) 151, 2339, 2461
EcoT14I CCWWGG 3 cut(s) 1287, 1500, 2593
EcoT22I ATGCAT 1 cut(s) 2287
EcoT38I GRGCYC 3 cut(s) 556, 1455, 2477
ErhI CCWWGG 3 cut(s) 1287, 1500, 2593
Esp3I CGTCTC 2 cut(s) 128, 1668
FalI AAGNNNNNCTT 2 cut(s) 1775, 1807
FauI CCCGC 1 cut(s) 1306
FauNDI CATATG 2 cut(s) 77, 2469
FbaI TGATCA 2 cut(s) 1299, 2521
FblI GTMKAC 3 cut(s) 19, 487, 1479
Fnu4HI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1254, 1806, 2819
FokI GGATG 3 cut(s) 458, 858, 2078
FriOI GRGCYC 3 cut(s) 556, 1455, 2477
Fsp4HI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1254, 1806, 2819
FspBI CTAG 5 cut(s) 6, 57, 861, 2358, 2646
GlaI GCGC 2 cut(s) 1380, 2817
GluI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1254, 1806, 2819
GsuI CTGGAG 1 cut(s) 1960
HaeII RGCGCY 1 cut(s) 2819
HaeIII GGCC 6 cut(s) 856, 1427, 1746, 2155, 2592, 2685
HapII CCGG 2 cut(s) 442, 1802
HhaI GCGC 2 cut(s) 1381, 2818
Hin6I GCGC 2 cut(s) 1379, 2816
HinP1I GCGC 2 cut(s) 1379, 2816
HincII GTYRAC 4 cut(s) 96, 509, 793, 1212
HindII GTYRAC 4 cut(s) 96, 509, 793, 1212
HindIII AAGCTT 2 cut(s) 278, 1294
HpaI GTTAAC 1 cut(s) 793
HpaII CCGG 2 cut(s) 442, 1802
HphI GGTGA 6 cut(s) 947, 1168, 2181, 2405, 2531, 2927
Hpy188I TCNGA 6 cut(s) 695, 1450, 1825, 2065, 2694, 2773
Hpy99I CGWCG 1 cut(s) 1368
HpyAV CCTTC 7 cut(s) 190, 317, 589, 592, 707, 2213, 2346
HpyCH4III ACNGT 7 cut(s) 314, 522, 1733, 1876, 2197, 2769, 2856
HpyCH4IV ACGT 4 cut(s) 22, 121, 1883, 2212
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 659, 1618, 1811, 2207, 2382
HpyF3I CTNAG 7 cut(s) 137, 365, 654, 800, 1152, 1449, 1965
HpySE526I ACGT 4 cut(s) 22, 121, 1883, 2212
HspAI GCGC 2 cut(s) 1379, 2816
KroI GCCGGC 1 cut(s) 1801
KroNI GCCGGC 1 cut(s) 1803
Ksp22I TGATCA 2 cut(s) 1299, 2521
KspAI GTTAAC 1 cut(s) 793
LmnI GCTCC 6 cut(s) 37, 527, 1759, 1941, 2454, 2555
Lsp1109I GCAGC 5 cut(s) 552, 1018, 1109, 1817, 2805
MabI ACCWGGT 1 cut(s) 2461
MaeI CTAG 5 cut(s) 6, 57, 861, 2358, 2646
MaeII ACGT 4 cut(s) 22, 121, 1883, 2212
MaeIII GTNAC 6 cut(s) 115, 389, 899, 1018, 1721, 2763
MflI RGATCY 2 cut(s) 148, 2080
MhlI GDGCHC 6 cut(s) 423, 556, 1455, 1870, 1946, 2477
MlsI TGGCCA 2 cut(s) 856, 2685
MluNI TGGCCA 2 cut(s) 856, 2685
MlyI GAGTC 4 cut(s) 185, 1429, 2633, 2951
MmeI TCCRAC 6 cut(s) 209, 973, 1520, 1760, 1859, 2413
Mox20I TGGCCA 2 cut(s) 856, 2685
Mph1103I ATGCAT 1 cut(s) 2287
MroNI GCCGGC 1 cut(s) 1801
MroXI GAANNNNTTC 3 cut(s) 51, 267, 1245
MscI TGGCCA 2 cut(s) 856, 2685
MslI CAYNNNNRTG 5 cut(s) 833, 1380, 1924, 2166, 2616
Msp20I TGGCCA 2 cut(s) 856, 2685
MspI CCGG 2 cut(s) 442, 1802
MspR9I CCNGG 4 cut(s) 153, 443, 2341, 2463
Mva1269I GAATGC 4 cut(s) 830, 1621, 1803, 2607
MvaI CCWGG 3 cut(s) 153, 2341, 2463
MvnI CGCG 1 cut(s) 1381
MwoI GCNNNNNNNGC 6 cut(s) 248, 659, 1618, 1811, 2207, 2382
NaeI GCCGGC 1 cut(s) 1803
NciI CCSGG 1 cut(s) 443
NcoI CCATGG 1 cut(s) 1500
NdeI CATATG 2 cut(s) 77, 2469
NgoMIV GCCGGC 1 cut(s) 1801
NlaIV GGNNCC 2 cut(s) 150, 2456
NmuCI GTSAC 4 cut(s) 115, 1018, 1721, 2763
NsiI ATGCAT 1 cut(s) 2287
NspI RCATGY 7 cut(s) 257, 472, 791, 1379, 1436, 2507, 2888
NspV TTCGAA 1 cut(s) 1122
PciI ACATGT 3 cut(s) 253, 787, 1432
PcsI WCGNNNNNNNCGW 1 cut(s) 2209
PctI GAATGC 4 cut(s) 830, 1621, 1803, 2607
PdiI GCCGGC 1 cut(s) 1803
PdmI GAANNNNTTC 3 cut(s) 51, 267, 1245
PfeI GAWTC 6 cut(s) 575, 696, 926, 1266, 2273, 2744
PflMI CCANNNNNTGG 1 cut(s) 1341
PfoI TCCNGGA 2 cut(s) 151, 2339
PkrI GCNGC 8 cut(s) 143, 474, 542, 1033, 1099, 1255, 1807, 2820
PleI GAGTC 4 cut(s) 184, 1428, 2633, 2951
PmaCI CACGTG 1 cut(s) 2213
PmlI CACGTG 1 cut(s) 2213
PpsI GAGTC 4 cut(s) 184, 1428, 2633, 2951
Ppu21I YACGTR 1 cut(s) 2213
PpuMI RGGWCCY 1 cut(s) 2261
PscI ACATGT 3 cut(s) 253, 787, 1432
PshBI ATTAAT 2 cut(s) 293, 1955
PsiI TTATAA 2 cut(s) 1461, 1683
Psp1406I AACGTT 1 cut(s) 1883
Psp5II RGGWCCY 1 cut(s) 2261
Psp6I CCWGG 3 cut(s) 151, 2339, 2461
PspCI CACGTG 1 cut(s) 2213
PspGI CCWGG 3 cut(s) 151, 2339, 2461
PspN4I GGNNCC 2 cut(s) 150, 2456
PspPI GGNCC 3 cut(s) 2071, 2261, 2669
PspPPI RGGWCCY 1 cut(s) 2261
PstNI CAGNNNCTG 2 cut(s) 1922, 2546
PsuI RGATCY 2 cut(s) 148, 2080
RsaI GTAC 8 cut(s) 131, 435, 463, 720, 1187, 2107, 2778, 2997
RsaNI GTAC 8 cut(s) 130, 434, 462, 719, 1186, 2106, 2777, 2996
RseI CAYNNNNRTG 5 cut(s) 833, 1380, 1924, 2166, 2616
SatI GCNGC 8 cut(s) 142, 473, 541, 1032, 1098, 1254, 1806, 2819
Sau96I GGNCC 3 cut(s) 2071, 2261, 2669
ScaI AGTACT 1 cut(s) 720
SchI GAGTC 4 cut(s) 185, 1429, 2633, 2951
ScrFI CCNGG 4 cut(s) 153, 443, 2341, 2463
SduI GDGCHC 6 cut(s) 423, 556, 1455, 1870, 1946, 2477
SexAI ACCWGGT 1 cut(s) 2461
SfcI CTRYAG 2 cut(s) 2193, 2559
SfuI TTCGAA 1 cut(s) 1122
SinI GGWCC 3 cut(s) 2071, 2261, 2669
SmiMI CAYNNNNRTG 5 cut(s) 833, 1380, 1924, 2166, 2616
SmlI CTYRAG 4 cut(s) 125, 356, 914, 1816
SmoI CTYRAG 4 cut(s) 125, 356, 914, 1816
SsiI CCGC 7 cut(s) 141, 459, 472, 1254, 1313, 2216, 2415
SspI AATATT 2 cut(s) 86, 1396
SspMI CTAG 5 cut(s) 6, 57, 861, 2358, 2646
StyD4I CCNGG 4 cut(s) 151, 441, 2339, 2461
StyI CCWWGG 3 cut(s) 1287, 1500, 2593
TaaI ACNGT 7 cut(s) 314, 522, 1733, 1876, 2197, 2769, 2856
TaiI ACGT 4 cut(s) 25, 124, 1886, 2215
TaqI TCGA 9 cut(s) 376, 550, 1110, 1122, 1264, 1584, 1648, 2041, 2280
TatI WGTACW 3 cut(s) 718, 1185, 2776
TauI GCSGC 3 cut(s) 144, 475, 1256
TfiI GAWTC 6 cut(s) 575, 696, 926, 1266, 2273, 2744
TscAI CASTG 6 cut(s) 319, 804, 1027, 1109, 1342, 1489
TseFI GTSAC 4 cut(s) 115, 1018, 1721, 2763
TseI GCWGC 5 cut(s) 540, 1031, 1097, 1805, 2818
Tsp45I GTSAC 4 cut(s) 115, 1018, 1721, 2763
TspGWI ACGGA 1 cut(s) 2859
TspRI CASTG 6 cut(s) 319, 804, 1027, 1109, 1342, 1489
Van91I CCANNNNNTGG 1 cut(s) 1341
VpaK11BI GGWCC 3 cut(s) 2071, 2261, 2669
VspI ATTAAT 2 cut(s) 293, 1955
XapI RAATTY 6 cut(s) 51, 664, 877, 1146, 1276, 2037
XceI RCATGY 7 cut(s) 257, 472, 791, 1379, 1436, 2507, 2888
XcmI CCANNNNNNNNNTGG 1 cut(s) 1842
XmiI GTMKAC 3 cut(s) 19, 487, 1479
XmnI GAANNNNTTC 3 cut(s) 51, 267, 1245
XspI CTAG 5 cut(s) 6, 57, 861, 2358, 2646
ZrmI AGTACT 1 cut(s) 720
Zsp2I ATGCAT 1 cut(s) 2287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.