Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 210 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 245 |
| AciI | CCGC | 1 cut(s) | 177 |
| AclWI | GGATC | 3 cut(s) | 57, 313, 575 |
| AcoI | YGGCCR | 1 cut(s) | 559 |
| AcuI | CTGAAG | 1 cut(s) | 549 |
| AcyI | GRCGYC | 1 cut(s) | 485 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 72, 245 |
| AgsI | TTSAA | 2 cut(s) | 102, 543 |
| AluBI | AGCT | 1 cut(s) | 117 |
| AluI | AGCT | 1 cut(s) | 117 |
| Alw26I | GTCTC | 1 cut(s) | 84 |
| AlwI | GGATC | 3 cut(s) | 57, 313, 575 |
| AoxI | GGCC | 2 cut(s) | 40, 559 |
| AspS9I | GGNCC | 2 cut(s) | 434, 628 |
| AsuC2I | CCSGG | 1 cut(s) | 626 |
| AsuHPI | GGTGA | 2 cut(s) | 200, 260 |
| AvaII | GGWCC | 2 cut(s) | 434, 628 |
| BalI | TGGCCA | 1 cut(s) | 561 |
| BanII | GRGCYC | 1 cut(s) | 239 |
| BcnI | CCSGG | 1 cut(s) | 626 |
| BcoDI | GTCTC | 1 cut(s) | 84 |
| BfaI | CTAG | 1 cut(s) | 120 |
| BfmI | CTRYAG | 2 cut(s) | 321, 667 |
| Bme1390I | CCNGG | 1 cut(s) | 626 |
| Bme18I | GGWCC | 2 cut(s) | 434, 628 |
| BmgT120I | GGNCC | 2 cut(s) | 434, 628 |
| BmiI | GGNNCC | 1 cut(s) | 630 |
| BmrFI | CCNGG | 1 cut(s) | 626 |
| BmsI | GCATC | 3 cut(s) | 17, 156, 486 |
| BoxI | GACNNNNGTC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 1 cut(s) | 626 |
| BsaBI | GATNNNNATC | 1 cut(s) | 282 |
| BsaHI | GRCGYC | 1 cut(s) | 485 |
| BsaJI | CCNNGG | 2 cut(s) | 341, 624 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 72, 245 |
| Bse1I | ACTGG | 1 cut(s) | 250 |
| Bse3DI | GCAATG | 2 cut(s) | 468, 579 |
| Bse8I | GATNNNNATC | 1 cut(s) | 282 |
| BseDI | CCNNGG | 2 cut(s) | 341, 624 |
| BseGI | GGATG | 1 cut(s) | 501 |
| BseJI | GATNNNNATC | 1 cut(s) | 282 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 72, 245 |
| BseMI | GCAATG | 2 cut(s) | 468, 579 |
| BseNI | ACTGG | 1 cut(s) | 250 |
| BseYI | CCCAGC | 1 cut(s) | 472 |
| BshFI | GGCC | 2 cut(s) | 42, 561 |
| BsiSI | CCGG | 1 cut(s) | 626 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 72, 245 |
| BsmAI | GTCTC | 1 cut(s) | 84 |
| BsnI | GGCC | 2 cut(s) | 42, 561 |
| Bsp1286I | GDGCHC | 1 cut(s) | 239 |
| Bsp143I | GATC | 5 cut(s) | 62, 128, 283, 305, 580 |
| BspACI | CCGC | 1 cut(s) | 177 |
| BspANI | GGCC | 2 cut(s) | 42, 561 |
| BspLI | GGNNCC | 1 cut(s) | 630 |
| BspPI | GGATC | 3 cut(s) | 57, 313, 575 |
| BsrDI | GCAATG | 2 cut(s) | 468, 579 |
| BsrI | ACTGG | 1 cut(s) | 250 |
| BssECI | CCNNGG | 2 cut(s) | 341, 624 |
| BssMI | GATC | 5 cut(s) | 62, 128, 283, 305, 580 |
| BssNI | GRCGYC | 1 cut(s) | 485 |
| BssT1I | CCWWGG | 1 cut(s) | 341 |
| Bst4CI | ACNGT | 1 cut(s) | 29 |
| BstACI | GRCGYC | 1 cut(s) | 485 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 70 |
| BstF5I | GGATG | 1 cut(s) | 501 |
| BstKTI | GATC | 5 cut(s) | 65, 131, 286, 308, 583 |
| BstMAI | GTCTC | 1 cut(s) | 84 |
| BstMBI | GATC | 5 cut(s) | 62, 128, 283, 305, 580 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 144 |
| BstNSI | RCATGY | 4 cut(s) | 269, 367, 647, 661 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 27 |
| BstSCI | CCNGG | 1 cut(s) | 624 |
| BstSFI | CTRYAG | 2 cut(s) | 321, 667 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 639 |
| BsuRI | GGCC | 2 cut(s) | 42, 561 |
| BtsCI | GGATG | 1 cut(s) | 501 |
| Cfr13I | GGNCC | 2 cut(s) | 434, 628 |
| CseI | GACGC | 1 cut(s) | 493 |
| CviJI | RGCY | 8 cut(s) | 42, 87, 117, 138, 237, 253, 561, 616 |
| CviKI_1 | RGCY | 8 cut(s) | 42, 87, 117, 138, 237, 253, 561, 616 |
| DpnI | GATC | 5 cut(s) | 64, 130, 285, 307, 582 |
| DpnII | GATC | 5 cut(s) | 62, 128, 283, 305, 580 |
| EaeI | YGGCCR | 1 cut(s) | 559 |
| Eco130I | CCWWGG | 1 cut(s) | 341 |
| Eco24I | GRGCYC | 1 cut(s) | 239 |
| Eco47I | GGWCC | 2 cut(s) | 434, 628 |
| Eco57I | CTGAAG | 1 cut(s) | 549 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 70 |
| EcoT14I | CCWWGG | 1 cut(s) | 341 |
| EcoT22I | ATGCAT | 1 cut(s) | 579 |
| EcoT38I | GRGCYC | 1 cut(s) | 239 |
| ErhI | CCWWGG | 1 cut(s) | 341 |
| FauNDI | CATATG | 1 cut(s) | 634 |
| FokI | GGATG | 1 cut(s) | 508 |
| FriOI | GRGCYC | 1 cut(s) | 239 |
| FspBI | CTAG | 1 cut(s) | 120 |
| GsaI | CCCAGC | 1 cut(s) | 476 |
| HaeIII | GGCC | 2 cut(s) | 42, 561 |
| HapII | CCGG | 1 cut(s) | 626 |
| HgaI | GACGC | 1 cut(s) | 493 |
| Hin1I | GRCGYC | 1 cut(s) | 485 |
| HinfI | GANTC | 3 cut(s) | 77, 383, 404 |
| HpaII | CCGG | 1 cut(s) | 626 |
| HphI | GGTGA | 2 cut(s) | 200, 260 |
| Hpy188I | TCNGA | 2 cut(s) | 152, 529 |
| Hpy188III | TCNNGA | 2 cut(s) | 17, 132 |
| HpyAV | CCTTC | 2 cut(s) | 108, 524 |
| HpyCH4III | ACNGT | 1 cut(s) | 29 |
| HpyCH4V | TGCA | 5 cut(s) | 8, 269, 499, 577, 661 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 144 |
| Hsp92I | GRCGYC | 1 cut(s) | 485 |
| Kzo9I | GATC | 5 cut(s) | 62, 128, 283, 305, 580 |
| LpnPI | CCDG | 8 cut(s) | 30, 58, 117, 231, 288, 486, 611, 639 |
| LweI | GCATC | 3 cut(s) | 17, 156, 486 |
| MaeI | CTAG | 1 cut(s) | 120 |
| MaeIII | GTNAC | 3 cut(s) | 170, 227, 506 |
| MalI | GATC | 5 cut(s) | 64, 130, 285, 307, 582 |
| MboI | GATC | 5 cut(s) | 62, 128, 283, 305, 580 |
| MboII | GAAGA | 4 cut(s) | 293, 389, 531, 690 |
| MhlI | GDGCHC | 1 cut(s) | 239 |
| MlsI | TGGCCA | 1 cut(s) | 561 |
| MluCI | AATT | 2 cut(s) | 222, 427 |
| MluNI | TGGCCA | 1 cut(s) | 561 |
| MlyI | GAGTC | 2 cut(s) | 86, 398 |
| MmeI | TCCRAC | 1 cut(s) | 181 |
| Mox20I | TGGCCA | 1 cut(s) | 561 |
| Mph1103I | ATGCAT | 1 cut(s) | 579 |
| MscI | TGGCCA | 1 cut(s) | 561 |
| MseI | TTAA | 1 cut(s) | 687 |
| MslI | CAYNNNNRTG | 1 cut(s) | 378 |
| Msp20I | TGGCCA | 1 cut(s) | 561 |
| MspA1I | CMGCKG | 1 cut(s) | 177 |
| MspI | CCGG | 1 cut(s) | 626 |
| MspR9I | CCNGG | 1 cut(s) | 626 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 144 |
| NciI | CCSGG | 1 cut(s) | 626 |
| NdeI | CATATG | 1 cut(s) | 634 |
| NdeII | GATC | 5 cut(s) | 62, 128, 283, 305, 580 |
| NlaIV | GGNNCC | 1 cut(s) | 630 |
| NmuCI | GTSAC | 2 cut(s) | 227, 506 |
| NsiI | ATGCAT | 1 cut(s) | 579 |
| NspI | RCATGY | 4 cut(s) | 269, 367, 647, 661 |
| PfeI | GAWTC | 1 cut(s) | 383 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 245 |
| PleI | GAGTC | 2 cut(s) | 85, 398 |
| PpsI | GAGTC | 2 cut(s) | 85, 398 |
| PshAI | GACNNNNGTC | 1 cut(s) | 27 |
| PsiI | TTATAA | 1 cut(s) | 210 |
| PspFI | CCCAGC | 1 cut(s) | 472 |
| PspN4I | GGNNCC | 1 cut(s) | 630 |
| PspPI | GGNCC | 2 cut(s) | 434, 628 |
| RseI | CAYNNNNRTG | 1 cut(s) | 378 |
| SaqAI | TTAA | 1 cut(s) | 687 |
| Sau3AI | GATC | 5 cut(s) | 62, 128, 283, 305, 580 |
| Sau96I | GGNCC | 2 cut(s) | 434, 628 |
| SchI | GAGTC | 2 cut(s) | 86, 398 |
| ScrFI | CCNGG | 1 cut(s) | 626 |
| SduI | GDGCHC | 1 cut(s) | 239 |
| SetI | ASST | 9 cut(s) | 61, 119, 157, 184, 190, 208, 355, 439, 681 |
| SfaNI | GCATC | 3 cut(s) | 17, 156, 486 |
| SfcI | CTRYAG | 2 cut(s) | 321, 667 |
| SinI | GGWCC | 2 cut(s) | 434, 628 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 378 |
| Sse9I | AATT | 2 cut(s) | 222, 427 |
| SsiI | CCGC | 1 cut(s) | 177 |
| SspMI | CTAG | 1 cut(s) | 120 |
| StyD4I | CCNGG | 1 cut(s) | 624 |
| StyI | CCWWGG | 1 cut(s) | 341 |
| TaaI | ACNGT | 1 cut(s) | 29 |
| TaqI | TCGA | 1 cut(s) | 61 |
| TasI | AATT | 2 cut(s) | 222, 427 |
| TfiI | GAWTC | 1 cut(s) | 383 |
| Tru1I | TTAA | 1 cut(s) | 687 |
| Tru9I | TTAA | 1 cut(s) | 687 |
| TseFI | GTSAC | 2 cut(s) | 227, 506 |
| Tsp45I | GTSAC | 2 cut(s) | 227, 506 |
| TspDTI | ATGAA | 3 cut(s) | 294, 390, 690 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 245 |
| VpaK11BI | GGWCC | 2 cut(s) | 434, 628 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 70 |
| XceI | RCATGY | 4 cut(s) | 269, 367, 647, 661 |
| XspI | CTAG | 1 cut(s) | 120 |
| Zsp2I | ATGCAT | 1 cut(s) | 579 |