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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 26 |
| AcsI | RAATTY | 1 cut(s) | 166 |
| AcyI | GRCGYC | 1 cut(s) | 48 |
| AluBI | AGCT | 1 cut(s) | 40 |
| AluI | AGCT | 1 cut(s) | 40 |
| AlwI | GGATC | 1 cut(s) | 26 |
| ApoI | RAATTY | 1 cut(s) | 166 |
| AsuHPI | GGTGA | 1 cut(s) | 38 |
| BfaI | CTAG | 1 cut(s) | 44 |
| BmsI | GCATC | 1 cut(s) | 102 |
| BsaHI | GRCGYC | 1 cut(s) | 48 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 68, 98 |
| Bse1I | ACTGG | 1 cut(s) | 7 |
| BseMII | CTCAG | 1 cut(s) | 72 |
| BseNI | ACTGG | 1 cut(s) | 7 |
| Bsp143I | GATC | 1 cut(s) | 31 |
| BspCNI | CTCAG | 1 cut(s) | 71 |
| BspPI | GGATC | 1 cut(s) | 26 |
| BsrI | ACTGG | 1 cut(s) | 7 |
| BssMI | GATC | 1 cut(s) | 31 |
| BssNI | GRCGYC | 1 cut(s) | 48 |
| BstACI | GRCGYC | 1 cut(s) | 48 |
| BstDEI | CTNAG | 2 cut(s) | 58, 159 |
| BstKTI | GATC | 1 cut(s) | 34 |
| BstMBI | GATC | 1 cut(s) | 31 |
| BtgZI | GCGATG | 1 cut(s) | 79 |
| CseI | GACGC | 1 cut(s) | 56 |
| CviJI | RGCY | 2 cut(s) | 40, 116 |
| CviKI_1 | RGCY | 2 cut(s) | 40, 116 |
| DdeI | CTNAG | 2 cut(s) | 58, 159 |
| DpnI | GATC | 1 cut(s) | 33 |
| DpnII | GATC | 1 cut(s) | 31 |
| FaiI | YATR | 2 cut(s) | 12, 89 |
| FspBI | CTAG | 1 cut(s) | 44 |
| HgaI | GACGC | 1 cut(s) | 56 |
| Hin1I | GRCGYC | 1 cut(s) | 48 |
| HinfI | GANTC | 1 cut(s) | 136 |
| HphI | GGTGA | 1 cut(s) | 38 |
| Hpy188I | TCNGA | 1 cut(s) | 107 |
| Hpy188III | TCNNGA | 1 cut(s) | 35 |
| HpyAV | CCTTC | 2 cut(s) | 11, 62 |
| HpyCH4V | TGCA | 2 cut(s) | 68, 123 |
| HpyF3I | CTNAG | 2 cut(s) | 58, 159 |
| Hsp92I | GRCGYC | 1 cut(s) | 48 |
| Kzo9I | GATC | 1 cut(s) | 31 |
| LpnPI | CCDG | 2 cut(s) | 20, 48 |
| LweI | GCATC | 1 cut(s) | 102 |
| MaeI | CTAG | 1 cut(s) | 44 |
| MalI | GATC | 1 cut(s) | 33 |
| MboI | GATC | 1 cut(s) | 31 |
| MboII | GAAGA | 1 cut(s) | 145 |
| MluCI | AATT | 3 cut(s) | 21, 101, 166 |
| MnlI | CCTC | 1 cut(s) | 106 |
| NdeII | GATC | 1 cut(s) | 31 |
| PfeI | GAWTC | 1 cut(s) | 136 |
| Sau3AI | GATC | 1 cut(s) | 31 |
| SetI | ASST | 1 cut(s) | 42 |
| SfaNI | GCATC | 1 cut(s) | 102 |
| SgeI | CNNG | 3 cut(s) | 19, 47, 56 |
| Sse9I | AATT | 3 cut(s) | 21, 101, 166 |
| SspMI | CTAG | 1 cut(s) | 44 |
| TasI | AATT | 3 cut(s) | 21, 101, 166 |
| TfiI | GAWTC | 1 cut(s) | 136 |
| XapI | RAATTY | 1 cut(s) | 166 |
| XspI | CTAG | 1 cut(s) | 44 |