FvH4_5g27012

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000220
Physical Location & Seq
Forward (+)
18325676 .. 18335106
9431 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27012.t1

Sequence Viewer

Length: 678 bp
ATGAAGCTTAAACTTCGAGATAGAGAAGCAGCTCATTCTCATGTGGACCAAAAAGATCCATTGGATGTCTTTGATATTGGGGAATTTTCTAACCCTGATATTGGTACCGAGTTTGATGATGAAGAAAGAGATCAACTTTATGAATGGATAAGGCCACTTCATCTAGATGACGAGGAACGTAATCCATCTCCTACAGTTGCTCGTGAAGCACGTGCGCAAGGAATAAATGTTGAACAAGTAATTCAAGAAGAGGTTATAGGACCTGATAGAGAAACTATCGATGCATTAGTGGGTCGAGCTAATATTTTAATTTTCTCTCGTGGAGAGTCTCGTGACTCACATGTTCCTTCCATGATAAATGAGAGCTCTAGTAGCACCAGATCAGGAGGCTCATCAAGCATCAGAGGTGATGATAATGGAGGTGATCATGGAGTTCAAGGTTACAGTGGAAATCAAGTTGATCAGATGAGTCCATTCACTTGTGAACCGCAATTTACACATGCAACACAAGATGGAGATCATGGAAGTAGGAGAGCAGGACCCGGCATCGGTGCTACAGGAAAAAATTATACACCAAGAGAAAGAGGCCAAGGAAGCATGTCAAGGCATGAAGATGATTCTTTATCTCCCTCTATTAACTCATTTGGTTATATATCATGCCTTACGGGGTGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

226

Amino Acids

24.69

Weight (kDa)

4.71

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 216
Acc65I GGTACC 1 cut(s) 104
AccB1I GGYRCC 1 cut(s) 104
AciI CCGC 1 cut(s) 488
AclWI GGATC 1 cut(s) 50
AcsI RAATTY 1 cut(s) 83
AcvI CACGTG 1 cut(s) 212
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 2 cut(s) 101, 548
AflIII ACRYGT 1 cut(s) 340
AgsI TTSAA 3 cut(s) 233, 245, 437
AluBI AGCT 4 cut(s) 7, 32, 299, 366
AluI AGCT 4 cut(s) 7, 32, 299, 366
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 1 cut(s) 333
AlwI GGATC 1 cut(s) 50
AoxI GGCC 2 cut(s) 152, 586
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 83
Asp718I GGTACC 1 cut(s) 104
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 3 cut(s) 46, 260, 539
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 419, 434
AvaII GGWCC 3 cut(s) 46, 260, 539
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 1 cut(s) 368
BauI CACGAG 3 cut(s) 201, 318, 330
BbrPI CACGTG 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 1 cut(s) 41
BccI CCATC 2 cut(s) 193, 506
BclI TGATCA 2 cut(s) 424, 460
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 2 cut(s) 164, 369
BfmI CTRYAG 2 cut(s) 192, 555
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 1 cut(s) 543
Bme18I GGWCC 3 cut(s) 46, 260, 539
BmgT120I GGNCC 3 cut(s) 46, 260, 539
BmiI GGNNCC 2 cut(s) 106, 541
BmrFI CCNGG 1 cut(s) 543
BmsI GCATC 3 cut(s) 271, 408, 555
BpuMI CCSGG 1 cut(s) 543
Bsa29I ATCGAT 1 cut(s) 279
BsaAI YACGTR 1 cut(s) 212
BsaBI GATNNNNATC 1 cut(s) 516
BsaJI CCNNGG 1 cut(s) 589
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 548
Bse8I GATNNNNATC 1 cut(s) 516
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 589
BseGI GGATG 1 cut(s) 70
BseJI GATNNNNATC 1 cut(s) 516
BseLI CCNNNNNNNGG 2 cut(s) 101, 548
BseXI GCAGC 1 cut(s) 41
BshFI GGCC 2 cut(s) 154, 588
BshNI GGYRCC 1 cut(s) 104
BshVI ATCGAT 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 1 cut(s) 543
BslI CCNNNNNNNGG 2 cut(s) 101, 548
BsmAI GTCTC 1 cut(s) 333
BsnI GGCC 2 cut(s) 154, 588
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 6 cut(s) 55, 130, 380, 424, 460, 517
BspACI CCGC 1 cut(s) 488
BspANI GGCC 2 cut(s) 154, 588
BspDI ATCGAT 1 cut(s) 279
BspLI GGNNCC 2 cut(s) 106, 541
BspPI GGATC 1 cut(s) 50
BspT107I GGYRCC 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 589
BssMI GATC 6 cut(s) 55, 130, 380, 424, 460, 517
BssSI CACGAG 3 cut(s) 201, 318, 330
BssT1I CCWWGG 1 cut(s) 589
Bst2BI CACGAG 3 cut(s) 201, 318, 330
Bst4CI ACNGT 2 cut(s) 196, 446
Bst6I CTCTTC 1 cut(s) 243
BstBAI YACGTR 1 cut(s) 212
BstF5I GGATG 1 cut(s) 70
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 6 cut(s) 58, 133, 383, 427, 463, 520
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 6 cut(s) 55, 130, 380, 424, 460, 517
BstMWI GCNNNNNNNGC 4 cut(s) 206, 372, 396, 594
BstNSI RCATGY 3 cut(s) 344, 503, 601
BstSCI CCNGG 1 cut(s) 541
BstSFI CTRYAG 2 cut(s) 192, 555
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 2 cut(s) 154, 588
BsuTUI ATCGAT 1 cut(s) 279
BtsCI GGATG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 451
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 3 cut(s) 46, 260, 539
ClaI ATCGAT 1 cut(s) 279
Csp6I GTAC 1 cut(s) 105
CviAII CATG 9 cut(s) 41, 341, 352, 428, 500, 521, 598, 608, 657
CviJI RGCY 7 cut(s) 7, 32, 154, 299, 366, 390, 588
CviKI_1 RGCY 7 cut(s) 7, 32, 154, 299, 366, 390, 588
CviQI GTAC 1 cut(s) 105
DpnI GATC 6 cut(s) 57, 132, 382, 426, 462, 519
DpnII GATC 6 cut(s) 55, 130, 380, 424, 460, 517
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Ecl136II GAGCTC 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 589
Eco24I GRGCYC 1 cut(s) 368
Eco47I GGWCC 3 cut(s) 46, 260, 539
Eco53kI GAGCTC 1 cut(s) 366
Eco72I CACGTG 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 366
EcoO109I RGGNCCY 2 cut(s) 260, 539
EcoT14I CCWWGG 1 cut(s) 589
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 589
FaeI CATG 9 cut(s) 44, 344, 355, 431, 503, 524, 601, 611, 660
FatI CATG 9 cut(s) 40, 340, 351, 427, 499, 520, 597, 607, 656
FbaI TGATCA 2 cut(s) 424, 460
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 1 cut(s) 77
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 2 cut(s) 164, 369
FspI TGCGCA 1 cut(s) 216
GlaI GCGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 30
HaeIII GGCC 2 cut(s) 154, 588
HapII CCGG 1 cut(s) 543
HhaI GCGC 1 cut(s) 217
Hin1II CATG 9 cut(s) 44, 344, 355, 431, 503, 524, 601, 611, 660
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 4 cut(s) 326, 335, 469, 617
HpaII CCGG 1 cut(s) 543
HphI GGTGA 2 cut(s) 419, 434
Hpy166II GTNNAC 2 cut(s) 46, 485
Hpy188I TCNGA 2 cut(s) 404, 465
Hpy188III TCNNGA 6 cut(s) 17, 164, 203, 245, 332, 384
Hpy8I GTNNAC 2 cut(s) 46, 485
HpyAV CCTTC 1 cut(s) 357
HpyCH4III ACNGT 2 cut(s) 196, 446
HpyCH4IV ACGT 2 cut(s) 178, 211
HpyCH4V TGCA 3 cut(s) 284, 503, 672
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 372, 396, 594
HpySE526I ACGT 2 cut(s) 178, 211
Hsp92II CATG 9 cut(s) 44, 344, 355, 431, 503, 524, 601, 611, 660
HspAI GCGC 1 cut(s) 215
KpnI GGTACC 1 cut(s) 108
Ksp22I TGATCA 2 cut(s) 424, 460
Kzo9I GATC 6 cut(s) 55, 130, 380, 424, 460, 517
LpnPI CCDG 7 cut(s) 108, 276, 369, 391, 522, 543, 556
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 3 cut(s) 271, 408, 555
MaeI CTAG 2 cut(s) 164, 369
MaeII ACGT 2 cut(s) 178, 211
MaeIII GTNAC 2 cut(s) 332, 440
MalI GATC 6 cut(s) 57, 132, 382, 426, 462, 519
MboI GATC 6 cut(s) 55, 130, 380, 424, 460, 517
MboII GAAGA 3 cut(s) 134, 260, 623
MflI RGATCY 1 cut(s) 55
MhlI GDGCHC 1 cut(s) 368
MluCI AATT 6 cut(s) 83, 240, 309, 491, 565, 673
MlyI GAGTC 3 cut(s) 329, 335, 478
MnlI CCTC 7 cut(s) 166, 244, 380, 398, 413, 578, 640
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 4 cut(s) 9, 308, 636, 676
MslI CAYNNNNRTG 3 cut(s) 39, 165, 612
MspI CCGG 1 cut(s) 543
MspR9I CCNGG 1 cut(s) 543
MwoI GCNNNNNNNGC 4 cut(s) 206, 372, 396, 594
NciI CCSGG 1 cut(s) 543
NdeII GATC 6 cut(s) 55, 130, 380, 424, 460, 517
NlaIII CATG 9 cut(s) 44, 344, 355, 431, 503, 524, 601, 611, 660
NlaIV GGNNCC 2 cut(s) 106, 541
NmuCI GTSAC 1 cut(s) 332
NsbI TGCGCA 1 cut(s) 216
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 3 cut(s) 344, 503, 601
PciI ACATGT 1 cut(s) 340
PcsI WCGNNNNNNNCGW 1 cut(s) 208
PfeI GAWTC 1 cut(s) 617
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 3 cut(s) 329, 334, 477
PmaCI CACGTG 1 cut(s) 212
PmlI CACGTG 1 cut(s) 212
PpsI GAGTC 3 cut(s) 329, 334, 477
Ppu21I YACGTR 1 cut(s) 212
PpuMI RGGWCCY 2 cut(s) 260, 539
PscI ACATGT 1 cut(s) 340
Psp124BI GAGCTC 1 cut(s) 368
Psp5II RGGWCCY 2 cut(s) 260, 539
PspCI CACGTG 1 cut(s) 212
PspN4I GGNNCC 2 cut(s) 106, 541
PspPI GGNCC 3 cut(s) 46, 260, 539
PspPPI RGGWCCY 2 cut(s) 260, 539
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
RseI CAYNNNNRTG 3 cut(s) 39, 165, 612
SacI GAGCTC 1 cut(s) 368
SaqAI TTAA 4 cut(s) 9, 308, 636, 676
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 6 cut(s) 55, 130, 380, 424, 460, 517
Sau96I GGNCC 3 cut(s) 46, 260, 539
SchI GAGTC 3 cut(s) 329, 335, 478
ScrFI CCNGG 1 cut(s) 543
SduI GDGCHC 1 cut(s) 368
SfaNI GCATC 3 cut(s) 271, 408, 555
SfcI CTRYAG 2 cut(s) 192, 555
SinI GGWCC 3 cut(s) 46, 260, 539
SmiMI CAYNNNNRTG 3 cut(s) 39, 165, 612
Sse9I AATT 6 cut(s) 83, 240, 309, 491, 565, 673
SsiI CCGC 1 cut(s) 488
SspI AATATT 1 cut(s) 304
SspMI CTAG 2 cut(s) 164, 369
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 1 cut(s) 541
StyI CCWWGG 1 cut(s) 589
TaaI ACNGT 2 cut(s) 196, 446
TaiI ACGT 2 cut(s) 181, 214
TaqI TCGA 3 cut(s) 16, 279, 295
TasI AATT 6 cut(s) 83, 240, 309, 491, 565, 673
TfiI GAWTC 1 cut(s) 617
Tru1I TTAA 4 cut(s) 9, 308, 636, 676
Tru9I TTAA 4 cut(s) 9, 308, 636, 676
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 5 cut(s) 17, 135, 149, 156, 624
TspRI CASTG 1 cut(s) 451
VpaK11BI GGWCC 3 cut(s) 46, 260, 539
XapI RAATTY 1 cut(s) 83
XbaI TCTAGA 1 cut(s) 163
XceI RCATGY 3 cut(s) 344, 503, 601
XspI CTAG 2 cut(s) 164, 369
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.