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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 154 |
| AciI | CCGC | 2 cut(s) | 29, 38 |
| AclWI | GGATC | 1 cut(s) | 37 |
| AcyI | GRCGYC | 1 cut(s) | 35 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 362 |
| AhlI | ACTAGT | 1 cut(s) | 190 |
| AluBI | AGCT | 1 cut(s) | 175 |
| AluI | AGCT | 1 cut(s) | 175 |
| AlwI | GGATC | 1 cut(s) | 37 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 312 |
| AoxI | GGCC | 1 cut(s) | 353 |
| ApeKI | GCWGC | 1 cut(s) | 175 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 164, 196 |
| AspS9I | GGNCC | 2 cut(s) | 14, 353 |
| AsuHPI | GGTGA | 2 cut(s) | 43, 257 |
| AvaII | GGWCC | 1 cut(s) | 14 |
| BbvI | GCAGC | 1 cut(s) | 162 |
| BccI | CCATC | 1 cut(s) | 337 |
| BceAI | ACGGC | 1 cut(s) | 184 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 31, 65, 73, 107 |
| BcuI | ACTAGT | 1 cut(s) | 190 |
| BfaI | CTAG | 1 cut(s) | 191 |
| BisI | GCNGC | 2 cut(s) | 38, 176 |
| BlsI | GCNGC | 2 cut(s) | 39, 177 |
| Bme18I | GGWCC | 1 cut(s) | 14 |
| BmgT120I | GGNCC | 2 cut(s) | 14, 353 |
| BmrI | ACTGGG | 1 cut(s) | 360 |
| BmuI | ACTGGG | 1 cut(s) | 360 |
| BoxI | GACNNNNGTC | 1 cut(s) | 193 |
| BsaHI | GRCGYC | 1 cut(s) | 35 |
| BsaJI | CCNNGG | 1 cut(s) | 200 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 136, 166 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 362 |
| Bse1I | ACTGG | 1 cut(s) | 355 |
| BseDI | CCNNGG | 1 cut(s) | 200 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 362 |
| BseNI | ACTGG | 1 cut(s) | 355 |
| BseXI | GCAGC | 1 cut(s) | 162 |
| BshFI | GGCC | 1 cut(s) | 355 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 362 |
| BsnI | GGCC | 1 cut(s) | 355 |
| Bsp143I | GATC | 1 cut(s) | 42 |
| BspACI | CCGC | 2 cut(s) | 29, 38 |
| BspANI | GGCC | 1 cut(s) | 355 |
| BspPI | GGATC | 1 cut(s) | 37 |
| BsrI | ACTGG | 1 cut(s) | 355 |
| BssECI | CCNNGG | 1 cut(s) | 200 |
| BssMI | GATC | 1 cut(s) | 42 |
| BssNI | GRCGYC | 1 cut(s) | 35 |
| Bst4CI | ACNGT | 2 cut(s) | 257, 331 |
| BstACI | GRCGYC | 1 cut(s) | 35 |
| BstC8I | GCNNGC | 2 cut(s) | 113, 320 |
| BstDEI | CTNAG | 1 cut(s) | 359 |
| BstDSI | CCRYGG | 1 cut(s) | 200 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 360 |
| BstKTI | GATC | 1 cut(s) | 45 |
| BstMBI | GATC | 1 cut(s) | 42 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 108, 233 |
| BstNSI | RCATGY | 1 cut(s) | 322 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 193 |
| BstV1I | GCAGC | 1 cut(s) | 162 |
| BsuRI | GGCC | 1 cut(s) | 355 |
| BtgI | CCRYGG | 1 cut(s) | 200 |
| BtsIMutI | CAGTG | 1 cut(s) | 348 |
| Cac8I | GCNNGC | 2 cut(s) | 113, 320 |
| CaiI | CAGNNNCTG | 1 cut(s) | 312 |
| Cfr13I | GGNCC | 2 cut(s) | 14, 353 |
| CseI | GACGC | 1 cut(s) | 43 |
| CviAII | CATG | 2 cut(s) | 79, 319 |
| CviJI | RGCY | 5 cut(s) | 115, 175, 227, 312, 355 |
| CviKI_1 | RGCY | 5 cut(s) | 115, 175, 227, 312, 355 |
| DdeI | CTNAG | 1 cut(s) | 359 |
| DpnI | GATC | 1 cut(s) | 44 |
| DpnII | GATC | 1 cut(s) | 42 |
| Eco47I | GGWCC | 1 cut(s) | 14 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 360 |
| FaeI | CATG | 2 cut(s) | 82, 322 |
| FaiI | YATR | 5 cut(s) | 80, 185, 275, 296, 320 |
| FatI | CATG | 2 cut(s) | 78, 318 |
| FblI | GTMKAC | 1 cut(s) | 154 |
| Fnu4HI | GCNGC | 2 cut(s) | 38, 176 |
| Fsp4HI | GCNGC | 2 cut(s) | 38, 176 |
| FspBI | CTAG | 1 cut(s) | 191 |
| GluI | GCNGC | 2 cut(s) | 38, 176 |
| HaeIII | GGCC | 1 cut(s) | 355 |
| HgaI | GACGC | 1 cut(s) | 43 |
| Hin1I | GRCGYC | 1 cut(s) | 35 |
| Hin1II | CATG | 2 cut(s) | 82, 322 |
| HinfI | GANTC | 1 cut(s) | 54 |
| HphI | GGTGA | 2 cut(s) | 43, 257 |
| Hpy166II | GTNNAC | 1 cut(s) | 155 |
| Hpy188I | TCNGA | 2 cut(s) | 47, 301 |
| Hpy8I | GTNNAC | 1 cut(s) | 155 |
| Hpy99I | CGWCG | 1 cut(s) | 257 |
| HpyCH4III | ACNGT | 2 cut(s) | 257, 331 |
| HpyCH4IV | ACGT | 1 cut(s) | 161 |
| HpyCH4V | TGCA | 1 cut(s) | 64 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 108, 233 |
| HpyF3I | CTNAG | 1 cut(s) | 359 |
| HpySE526I | ACGT | 1 cut(s) | 161 |
| Hsp92I | GRCGYC | 1 cut(s) | 35 |
| Hsp92II | CATG | 2 cut(s) | 82, 322 |
| Kzo9I | GATC | 1 cut(s) | 42 |
| LpnPI | CCDG | 2 cut(s) | 30, 336 |
| Lsp1109I | GCAGC | 1 cut(s) | 162 |
| MaeI | CTAG | 1 cut(s) | 191 |
| MaeII | ACGT | 1 cut(s) | 161 |
| MaeIII | GTNAC | 3 cut(s) | 31, 245, 346 |
| MalI | GATC | 1 cut(s) | 44 |
| MboI | GATC | 1 cut(s) | 42 |
| MboII | GAAGA | 3 cut(s) | 16, 49, 282 |
| MnlI | CCTC | 8 cut(s) | 5, 88, 126, 138, 257, 302, 356, 366 |
| MseI | TTAA | 1 cut(s) | 213 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 108, 233 |
| NdeII | GATC | 1 cut(s) | 42 |
| NlaIII | CATG | 2 cut(s) | 82, 322 |
| NmuCI | GTSAC | 3 cut(s) | 31, 245, 346 |
| NspI | RCATGY | 1 cut(s) | 322 |
| PaeI | GCATGC | 1 cut(s) | 322 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 167 |
| PfeI | GAWTC | 1 cut(s) | 54 |
| PkrI | GCNGC | 2 cut(s) | 39, 177 |
| PshAI | GACNNNNGTC | 1 cut(s) | 193 |
| PspPI | GGNCC | 2 cut(s) | 14, 353 |
| PstNI | CAGNNNCTG | 1 cut(s) | 312 |
| SaqAI | TTAA | 1 cut(s) | 213 |
| SatI | GCNGC | 2 cut(s) | 38, 176 |
| Sau3AI | GATC | 1 cut(s) | 42 |
| Sau96I | GGNCC | 2 cut(s) | 14, 353 |
| SetI | ASST | 6 cut(s) | 99, 149, 164, 177, 242, 268 |
| SinI | GGWCC | 1 cut(s) | 14 |
| SpeI | ACTAGT | 1 cut(s) | 190 |
| SphI | GCATGC | 1 cut(s) | 322 |
| SsiI | CCGC | 2 cut(s) | 29, 38 |
| SspMI | CTAG | 1 cut(s) | 191 |
| TaaI | ACNGT | 2 cut(s) | 257, 331 |
| TaiI | ACGT | 1 cut(s) | 164 |
| TaqI | TCGA | 2 cut(s) | 93, 128 |
| TauI | GCSGC | 1 cut(s) | 40 |
| TfiI | GAWTC | 1 cut(s) | 54 |
| Tru1I | TTAA | 1 cut(s) | 213 |
| Tru9I | TTAA | 1 cut(s) | 213 |
| TscAI | CASTG | 1 cut(s) | 355 |
| TseFI | GTSAC | 3 cut(s) | 31, 245, 346 |
| TseI | GCWGC | 1 cut(s) | 175 |
| Tsp45I | GTSAC | 3 cut(s) | 31, 245, 346 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspRI | CASTG | 1 cut(s) | 355 |
| VpaK11BI | GGWCC | 1 cut(s) | 14 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 360 |
| XceI | RCATGY | 1 cut(s) | 322 |
| XmiI | GTMKAC | 1 cut(s) | 154 |
| XspI | CTAG | 1 cut(s) | 191 |