Rw1G014540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000220
Physical Location & Seq
Reverse (-)
32465487 .. 32468628
3142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G014540.1

Sequence Viewer

Length: 1647 bp
ATGGCAAGCAGCGGTAGAGGTGGAAATGATCCAGCATGGAAGTACGCAACTCCCGTCGAAGGAAATCGAAATGGTACAACATGTATATTTTGTTTTGCCACTTTTAAAAGTGGAGGAATTACAAGATTGAAGAGTCATTTAGCGGGTTATGATCCTCACAAGAGTGTAAAGAAATGTGATAAGGTGCCACCGGAGATCAAAACAGAGGTGATAGCATGGATAAAGAGAAAAGATTCTATTAAGCAAGAAAAGGCAGCTGTTCAAGATGATATTAGAGAAGAACTCCGCAATGATTATCTTGGCAATCAAACAAGTCCTGGTGTAAAGCTTCCTACCTCCTATGAAATTCAGACTCGTTACTTAGAGAATGAGTACACAGAAATGAAAAGGTATGTGGATACTCACAGAGAAACCTGGAAAATATATGGATGCACTATCATGTGTGATGGATGGACTGGCCCAACCAAAATGTCAATTCTAAATTTCATGGTGTATTCAAAAGGTTCAACTGTGTTTTTGAAGTCTGTAGATGCTTCACATATGAAAAAAAATGCTCAGTACATCGAACTATTAAATGTTGTCCAGATTGTCACTGACAATGCATCAGCATTTAAAAAGGCTGGAAGGGAACTACAGAAAAAATATCCGCTATTCTGGACCCCGTGTGCTGCCCACTGCATCGATTTGATATTTGAAGATATCGGGAAGCAAGAGACTGTTTCCACTGTCGTCAAATGGGCTCAAAGTGTAACTAACTACATCTACAATCATGGTTGGGTACTGGCTGAAATGAGGAGCATTACAAAGGGAGATTTGATTCGACCGGGTAAAACTCGATTTGCGACAAATCACATTGCCATCGACAGCATTTTGAAGAAGAAATCAGATTTGAGAAAATTGTTTACAAGTAGCGCATGGAATGAAAATGCAGCGAGCAGGACCAGAGATGGCAAAAGGATAGAAAAAAGAGTCCTCGATGGGACATTTTGGGATGGCATGGAAGCAGTCCATAAAATTTATAAGCCATTGTATGAGATTCTTCGAATTGTTGACACAGAAATTCAGCCAACCATGCCCATATTATATATGAGAGGAAAGAAATGGGTGCTTAAAATCATCAATGATAGATGGGATAACACATTAAGTCAACCATTGCATGCAGCAGCACATTTCTTAAACCCAAATTTTCATTATCGACATGATGTTGGTTATAGCCCACGTTTCACAAACTCTCTGGCAACAATTGTCGAACAATTTAACATAAATGGAGAAATTGCAGATACATTTGTGGATGAGAGATGCAAAGGAATCATTTGGGTCAACCATAGCAAGAAAAACAAGAGAAACCAAAAATACAGAGTCTTATCTGAAGAAGTTGTTAAAAACCCAGAAGATAATTCAGATAGTGATGATGCGTGGGGTGGTACAGCAACTCCAGATAGTTCTGATGATGGTGGAGAGGGTGGAAGCGGAACAGGTGGTGGAGGTGAAAGATATGAATATGGGCAATCTTCTAGCGCAGTGGGATCTCAAATGAATCTCATGAAGGCAACAATCAATTTGGTTATGATGCCTATGGATATAATCAATATGGAGAAGTATATGATCAGTCATCTAGTTGGGTTCATCCTTATTATCCCCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT zf-BED

Protein Analysis

548

Amino Acids

62.11

Weight (kDa)

8.92

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-BED PF02892 10 - 47 9.3e-06 BED zinc finger
DUF659 PF04937 111 - 250 2.1e-41 Domain of unknown function (DUF659)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14882 FvH4_1g25271 FvH4_2g02251 FvH4_2g02251 FvH4_2g12511 FvH4_3g12392 FvH4_4g13981 FvH4_5g27011 FvH4_5g27012 FvH4_6g04722 FvH4_6g19751 FvH4_6g31371 FvH4_6g35011 FvH4_7g02741 FvH4_7g02741 FvH4_7g11332
malus_domestica MD00G1003900.v1.1 MD00G1004000.v1.1 MD10G1094800.v1.1 MD13G1044700.v1.1 MD13G1176700.v1.1 MD15G1343300.v1.1 MD15G1444800.v1.1
prunus_persica Prupe.1G199600_v2.0.a1 Prupe.1G215800_v2.0.a1 Prupe.3G104200_v2.0.a1
pyrus_communis pycom06g06750 pycom08g00120 pycom08g00130 pycom10g02160 pycom11g16040 pycom11g16050 pycom11g17730 pycom15g19390 pycom15g25050 pycom15g25060 pycom16g24700 pycom16g24710 pycom5153684g00040
rosa_chinensis RchiOBHm_Chr1g0361971 RchiOBHm_Chr1g0383751 RchiOBHm_Chr2g0121831 RchiOBHm_Chr2g0121841 RchiOBHm_Chr2g0168971 RchiOBHm_Chr4g0384811 RchiOBHm_Chr4g0385621 RchiOBHm_Chr5g0012611 RchiOBHm_Chr6g0271241 RchiOBHm_Chr6g0289011 RchiOBHm_Chr7g0216171 RchiOBHm_Chr7g0216181 RchiOBHm_Chr7g0239421
rosa_laevigata RLG00000001765 RLG00000002860 RLG00000003609 RLG00000004743 RLG00000018385 RLG00000022782 RLG00000024093 RLG00000035412
rosa_multiflora Rmu_sc0000239.1_g000042 Rmu_sc0000292.1_g000005 Rmu_sc0000414.1_g000004 Rmu_sc0000441.1_g000099 Rmu_sc0000578.1_g000002 Rmu_sc0000666.1_g000019 Rmu_sc0000690.1_g000008 Rmu_sc0000821.1_g000011 Rmu_sc0001068.1_g000021 Rmu_sc0001696.1_g000017 Rmu_sc0001851.1_g000006 Rmu_sc0001940.1_g000018 Rmu_sc0002322.1_g000025 Rmu_sc0002343.1_g000030 Rmu_sc0002351.1_g000020 Rmu_sc0002371.1_g000025 Rmu_sc0002530.1_g000003 Rmu_sc0003206.1_g000001 Rmu_sc0003556.1_g000020 Rmu_sc0003906.1_g000002 Rmu_sc0004122.1_g000002 Rmu_sc0004283.1_g000013 Rmu_sc0004657.1_g000053 Rmu_sc0004657.1_g000054 Rmu_sc0004920.1_g000006 Rmu_sc0004990.1_g000001 Rmu_sc0005046.1_g000011 Rmu_sc0005134.1_g000006 Rmu_sc0005281.1_g000004 Rmu_sc0005704.1_g000010 Rmu_sc0005767.1_g000009 Rmu_sc0005789.1_g000031 Rmu_sc0005849.1_g000010 Rmu_sc0006736.1_g000020 Rmu_sc0006754.1_g000001 Rmu_sc0007663.1_g000010 Rmu_sc0007705.1_g000005 Rmu_sc0007883.1_g000003 Rmu_sc0007941.1_g000003 Rmu_sc0009618.1_g000009 Rmu_sc0009840.1_g000007 Rmu_sc0010800.1_g000007 Rmu_sc0011620.1_g000005 Rmu_sc0013423.1_g000002 Rmu_sc0014544.1_g000001 Rmu_sc0015323.1_g000004 Rmu_sc0023737.1_g000001 Rmu_sc0024470.1_g000005 Rmu_sc0032114.1_g000001 Rmu_ssc0000372.1_g000050 Rmu_ssc0000420.1_g000006
rosa_roxburghii Rroxscaffold_1G00014580 Rroxscaffold_1G00050290 Rroxscaffold_2G00093920 Rroxscaffold_2G00131720 Rroxscaffold_5G00351350 Rroxscaffold_5G00386210 Rroxscaffold_6G00392840 Rroxscaffold_6G00404480 Rroxscaffold_6G00405300
rosa_rugosa Rorug02G0533800 Rorug03G0200600 Rorug05G0329600 Rorug07G0139200 Rorug07G0341500 Rorug07G0341600 Rorug07G0341700 Rorug07G0341800
rosa_samantha Rh2BG114900 Rh2BG222900 Rh2DG253700 Rh3CG200000 Rh4BG186200 Rh4DG156200 Rh5AG145800 Rh5BG370600 Rh6AG123800 Rh6BG424900 Rh6DG105300 Rh7AG381900 Rh7CG288100 Rh7CG401400
rosa_wichuraiana Rw0G008270 Rw1G014540 Rw2G043790 Rw6G021690 Rw7G030100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1020
AccB1I GGYRCC 1 cut(s) 184
AciI CCGC 5 cut(s) 12, 143, 286, 647, 1470
AclWI GGATC 3 cut(s) 23, 146, 1534
AcsI RAATTY 5 cut(s) 345, 481, 1014, 1059, 1183
AcuI CTGAAG 1 cut(s) 1389
AfaI GTAC 6 cut(s) 44, 76, 374, 560, 780, 1426
AfiI CCNNNNNNNGG 1 cut(s) 59
AflIII ACRYGT 1 cut(s) 80
AgsI TTSAA 7 cut(s) 130, 263, 498, 507, 520, 695, 874
AjnI CCWGG 2 cut(s) 316, 413
AleI CACNNNNGTG 1 cut(s) 162
AluBI AGCT 2 cut(s) 257, 328
AluI AGCT 2 cut(s) 257, 328
Alw26I GTCTC 1 cut(s) 707
AlwI GGATC 3 cut(s) 23, 146, 1534
AoxI GGCC 1 cut(s) 457
ApeKI GCWGC 6 cut(s) 9, 254, 668, 929, 1160, 1163
ApoI RAATTY 5 cut(s) 345, 481, 1014, 1059, 1183
Asp700I GAANNNNTTC 1 cut(s) 232
AspLEI GCGC 2 cut(s) 914, 1520
AspS9I GGNCC 3 cut(s) 458, 657, 939
AsuC2I CCSGG 1 cut(s) 825
AsuHPI GGTGA 2 cut(s) 220, 1499
AsuII TTCGAA 1 cut(s) 1042
AvaII GGWCC 2 cut(s) 657, 939
BaeI ACNNNNGTAYC 2 cut(s) 1272, 1305
BanI GGYRCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 742
BbvI GCAGC 6 cut(s) 21, 266, 655, 941, 1172, 1175
BccI CCATC 8 cut(s) 440, 444, 866, 941, 971, 986, 1122, 1445
BciT130I CCWGG 2 cut(s) 318, 415
BciVI GTATCC 1 cut(s) 391
BclI TGATCA 1 cut(s) 1605
BcnI CCSGG 1 cut(s) 825
BcoDI GTCTC 1 cut(s) 707
BfaI CTAG 2 cut(s) 1515, 1616
BfmI CTRYAG 2 cut(s) 525, 632
BfuI GTATCC 1 cut(s) 391
BisI GCNGC 6 cut(s) 10, 255, 669, 930, 1161, 1164
BlsI GCNGC 6 cut(s) 11, 256, 670, 931, 1162, 1165
Bme1390I CCNGG 3 cut(s) 318, 415, 825
Bme18I GGWCC 2 cut(s) 657, 939
BmgT120I GGNCC 3 cut(s) 458, 657, 939
BmiI GGNNCC 2 cut(s) 186, 659
BmrFI CCNGG 3 cut(s) 318, 415, 825
BmsI GCATC 7 cut(s) 419, 520, 611, 687, 1289, 1402, 1560
BplI GAGNNNNNCTC 2 cut(s) 267, 299
BpmI CTGGAG 1 cut(s) 1419
Bpu14I TTCGAA 1 cut(s) 1042
BpuMI CCSGG 1 cut(s) 825
Bsa29I ATCGAT 1 cut(s) 681
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 1417, 1447
Bsc4I CCNNNNNNNGG 1 cut(s) 59
Bse1I ACTGG 2 cut(s) 460, 786
Bse3DI GCAATG 3 cut(s) 295, 852, 1151
BseBI CCWGG 2 cut(s) 318, 415
BseCI ATCGAT 1 cut(s) 681
BseGI GGATG 5 cut(s) 434, 455, 997, 1297, 1626
BseLI CCNNNNNNNGG 1 cut(s) 59
BseMI GCAATG 3 cut(s) 295, 852, 1151
BseMII CTCAG 1 cut(s) 569
BseNI ACTGG 2 cut(s) 460, 786
BseRI GAGGAG 1 cut(s) 808
BseXI GCAGC 6 cut(s) 21, 266, 655, 941, 1172, 1175
Bsh1285I CGRYCG 1 cut(s) 824
BshFI GGCC 1 cut(s) 459
BshNI GGYRCC 1 cut(s) 184
BshVI ATCGAT 1 cut(s) 681
BsiEI CGRYCG 1 cut(s) 824
BsiSI CCGG 2 cut(s) 191, 824
BslFI GGGAC 1 cut(s) 994
BslI CCNNNNNNNGG 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 707
BsmFI GGGAC 1 cut(s) 994
BsnI GGCC 1 cut(s) 459
Bsp119I TTCGAA 1 cut(s) 1042
Bsp1286I GDGCHC 1 cut(s) 742
Bsp143I GATC 5 cut(s) 28, 151, 195, 1526, 1605
BspACI CCGC 5 cut(s) 12, 143, 286, 647, 1470
BspANI GGCC 1 cut(s) 459
BspCNI CTCAG 1 cut(s) 568
BspDI ATCGAT 1 cut(s) 681
BspHI TCATGA 1 cut(s) 1542
BspLI GGNNCC 2 cut(s) 186, 659
BspPI GGATC 3 cut(s) 23, 146, 1534
BspT104I TTCGAA 1 cut(s) 1042
BspT107I GGYRCC 1 cut(s) 184
BsrDI GCAATG 3 cut(s) 295, 852, 1151
BsrI ACTGG 2 cut(s) 460, 786
BssMI GATC 5 cut(s) 28, 151, 195, 1526, 1605
Bst2UI CCWGG 2 cut(s) 318, 415
Bst4CI ACNGT 3 cut(s) 511, 718, 727
Bst6I CTCTTC 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 1042
BstC8I GCNNGC 3 cut(s) 7, 934, 1158
BstDEI CTNAG 2 cut(s) 361, 555
BstF5I GGATG 5 cut(s) 434, 455, 997, 1297, 1626
BstHHI GCGC 2 cut(s) 914, 1520
BstKTI GATC 5 cut(s) 31, 154, 198, 1529, 1608
BstMAI GTCTC 1 cut(s) 707
BstMBI GATC 5 cut(s) 28, 151, 195, 1526, 1605
BstMCI CGRYCG 1 cut(s) 824
BstMWI GCNNNNNNNGC 1 cut(s) 1072
BstNI CCWGG 2 cut(s) 318, 415
BstNSI RCATGY 2 cut(s) 84, 1160
BstSCI CCNGG 3 cut(s) 316, 413, 823
BstSFI CTRYAG 2 cut(s) 525, 632
BstV1I GCAGC 6 cut(s) 21, 266, 655, 941, 1172, 1175
BstX2I RGATCY 1 cut(s) 1526
BstYI RGATCY 1 cut(s) 1526
Bsu15I ATCGAT 1 cut(s) 681
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 1 cut(s) 459
BsuTUI ATCGAT 1 cut(s) 681
BtsCI GGATG 5 cut(s) 434, 455, 997, 1297, 1626
BtsI GCAGTG 2 cut(s) 673, 1527
BtsIMutI CAGTG 4 cut(s) 591, 673, 723, 1527
Cac8I GCNNGC 3 cut(s) 7, 934, 1158
CciI TCATGA 1 cut(s) 1542
CfoI GCGC 2 cut(s) 914, 1520
Cfr13I GGNCC 3 cut(s) 458, 657, 939
ClaI ATCGAT 1 cut(s) 681
Csp6I GTAC 6 cut(s) 43, 75, 373, 559, 779, 1425
CviJI RGCY 9 cut(s) 257, 328, 459, 620, 740, 785, 1024, 1066, 1215
CviKI_1 RGCY 9 cut(s) 257, 328, 459, 620, 740, 785, 1024, 1066, 1215
CviQI GTAC 6 cut(s) 43, 75, 373, 559, 779, 1425
DdeI CTNAG 2 cut(s) 361, 555
DpnI GATC 5 cut(s) 30, 153, 197, 1528, 1607
DpnII GATC 5 cut(s) 28, 151, 195, 1526, 1605
DraI TTTAAA 2 cut(s) 106, 613
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Eco24I GRGCYC 1 cut(s) 742
Eco32I GATATC 1 cut(s) 700
Eco47I GGWCC 2 cut(s) 657, 939
Eco57I CTGAAG 1 cut(s) 1389
EcoRII CCWGG 2 cut(s) 316, 413
EcoRV GATATC 1 cut(s) 700
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 742
FaqI GGGAC 1 cut(s) 994
FauI CCCGC 1 cut(s) 136
FauNDI CATATG 1 cut(s) 540
FbaI TGATCA 1 cut(s) 1605
Fnu4HI GCNGC 6 cut(s) 10, 255, 669, 930, 1161, 1164
FokI GGATG 5 cut(s) 441, 462, 1004, 1304, 1613
FriOI GRGCYC 1 cut(s) 742
Fsp4HI GCNGC 6 cut(s) 10, 255, 669, 930, 1161, 1164
FspBI CTAG 2 cut(s) 1515, 1616
GlaI GCGC 2 cut(s) 913, 1519
GluI GCNGC 6 cut(s) 10, 255, 669, 930, 1161, 1164
GsuI CTGGAG 1 cut(s) 1419
HaeIII GGCC 1 cut(s) 459
HapII CCGG 2 cut(s) 191, 824
HhaI GCGC 2 cut(s) 914, 1520
Hin6I GCGC 2 cut(s) 912, 1518
HinP1I GCGC 2 cut(s) 912, 1518
HincII GTYRAC 3 cut(s) 1051, 1148, 1321
HindII GTYRAC 3 cut(s) 1051, 1148, 1321
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 9 cut(s) 133, 233, 352, 817, 969, 1036, 1308, 1359, 1537
HpaII CCGG 2 cut(s) 191, 824
HphI GGTGA 2 cut(s) 220, 1499
Hpy166II GTNNAC 5 cut(s) 375, 903, 1051, 1148, 1321
Hpy188I TCNGA 5 cut(s) 351, 886, 1369, 1402, 1447
Hpy188III TCNNGA 6 cut(s) 263, 583, 655, 703, 1436, 1543
Hpy8I GTNNAC 5 cut(s) 375, 903, 1051, 1148, 1321
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 3 cut(s) 53, 618, 1540
HpyCH4III ACNGT 3 cut(s) 511, 718, 727
HpyCH4IV ACGT 1 cut(s) 1219
HpyCH4V TGCA 8 cut(s) 432, 602, 678, 929, 1156, 1160, 1277, 1302
HpyF10VI GCNNNNNNNGC 1 cut(s) 1072
HpyF3I CTNAG 2 cut(s) 361, 555
HpySE526I ACGT 1 cut(s) 1219
HspAI GCGC 2 cut(s) 912, 1518
Ksp22I TGATCA 1 cut(s) 1605
Kzo9I GATC 5 cut(s) 28, 151, 195, 1526, 1605
LmnI GCTCC 1 cut(s) 795
Lsp1109I GCAGC 6 cut(s) 21, 266, 655, 941, 1172, 1175
LweI GCATC 7 cut(s) 419, 520, 611, 687, 1289, 1402, 1560
MaeI CTAG 2 cut(s) 1515, 1616
MaeII ACGT 1 cut(s) 1219
MaeIII GTNAC 3 cut(s) 356, 589, 748
MalI GATC 5 cut(s) 30, 153, 197, 1528, 1607
MboI GATC 5 cut(s) 28, 151, 195, 1526, 1605
MboII GAAGA 9 cut(s) 142, 290, 707, 886, 889, 1031, 1382, 1403, 1503
MfeI CAATTG 1 cut(s) 1242
MflI RGATCY 1 cut(s) 1526
MhlI GDGCHC 1 cut(s) 742
MlyI GAGTC 4 cut(s) 142, 346, 978, 1368
Mph1103I ATGCAT 1 cut(s) 604
MroXI GAANNNNTTC 1 cut(s) 232
MseI TTAA 9 cut(s) 105, 240, 572, 612, 1110, 1142, 1175, 1257, 1380
MslI CAYNNNNRTG 3 cut(s) 162, 380, 437
MspA1I CMGCKG 2 cut(s) 12, 257
MspI CCGG 2 cut(s) 191, 824
MspR9I CCNGG 3 cut(s) 318, 415, 825
MunI CAATTG 1 cut(s) 1242
MvaI CCWGG 2 cut(s) 318, 415
MwoI GCNNNNNNNGC 1 cut(s) 1072
NciI CCSGG 1 cut(s) 825
NdeI CATATG 1 cut(s) 540
NdeII GATC 5 cut(s) 28, 151, 195, 1526, 1605
NlaIV GGNNCC 2 cut(s) 186, 659
NmuCI GTSAC 1 cut(s) 589
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 2 cut(s) 84, 1160
NspV TTCGAA 1 cut(s) 1042
OliI CACNNNNGTG 1 cut(s) 162
PaeI GCATGC 1 cut(s) 1160
PagI TCATGA 1 cut(s) 1542
PciI ACATGT 1 cut(s) 80
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 232
PfeI GAWTC 5 cut(s) 233, 817, 1036, 1308, 1537
PkrI GCNGC 6 cut(s) 11, 256, 670, 931, 1162, 1165
PleI GAGTC 4 cut(s) 141, 346, 977, 1367
PpsI GAGTC 4 cut(s) 141, 346, 977, 1367
PscI ACATGT 1 cut(s) 80
PsiI TTATAA 1 cut(s) 1020
Psp6I CCWGG 2 cut(s) 316, 413
PspGI CCWGG 2 cut(s) 316, 413
PspN4I GGNNCC 2 cut(s) 186, 659
PspPI GGNCC 3 cut(s) 458, 657, 939
PsuI RGATCY 1 cut(s) 1526
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 6 cut(s) 44, 76, 374, 560, 780, 1426
RsaNI GTAC 6 cut(s) 43, 75, 373, 559, 779, 1425
RseI CAYNNNNRTG 3 cut(s) 162, 380, 437
SaqAI TTAA 9 cut(s) 105, 240, 572, 612, 1110, 1142, 1175, 1257, 1380
SatI GCNGC 6 cut(s) 10, 255, 669, 930, 1161, 1164
Sau3AI GATC 5 cut(s) 28, 151, 195, 1526, 1605
Sau96I GGNCC 3 cut(s) 458, 657, 939
SchI GAGTC 4 cut(s) 142, 346, 978, 1368
ScrFI CCNGG 3 cut(s) 318, 415, 825
SduI GDGCHC 1 cut(s) 742
SfaNI GCATC 7 cut(s) 419, 520, 611, 687, 1289, 1402, 1560
SfcI CTRYAG 2 cut(s) 525, 632
SfuI TTCGAA 1 cut(s) 1042
SinI GGWCC 2 cut(s) 657, 939
SmiMI CAYNNNNRTG 3 cut(s) 162, 380, 437
SphI GCATGC 1 cut(s) 1160
SsiI CCGC 5 cut(s) 12, 143, 286, 647, 1470
SspMI CTAG 2 cut(s) 1515, 1616
StyD4I CCNGG 3 cut(s) 316, 413, 823
TaaI ACNGT 3 cut(s) 511, 718, 727
TaiI ACGT 1 cut(s) 1222
TatI WGTACW 2 cut(s) 372, 558
TfiI GAWTC 5 cut(s) 233, 817, 1036, 1308, 1537
Tru1I TTAA 9 cut(s) 105, 240, 572, 612, 1110, 1142, 1175, 1257, 1380
Tru9I TTAA 9 cut(s) 105, 240, 572, 612, 1110, 1142, 1175, 1257, 1380
TscAI CASTG 4 cut(s) 598, 680, 730, 1527
TseFI GTSAC 1 cut(s) 589
TseI GCWGC 6 cut(s) 9, 254, 668, 929, 1160, 1163
Tsp45I GTSAC 1 cut(s) 589
TspRI CASTG 4 cut(s) 598, 680, 730, 1527
VpaK11BI GGWCC 2 cut(s) 657, 939
XapI RAATTY 5 cut(s) 345, 481, 1014, 1059, 1183
XceI RCATGY 2 cut(s) 84, 1160
XmnI GAANNNNTTC 1 cut(s) 232
XspI CTAG 2 cut(s) 1515, 1616
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.