FvH4_2g02580

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000108
Physical Location & Seq
Forward (+)
2064340 .. 2066961
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02580.t1

Sequence Viewer

Length: 1581 bp
ATGGTGTCTTACGAGTACGTTCTCGGCGGAGTCCTGGCTTCTCTGCTCGGTTCTGTTTTCTTCATGATCATCACCTCCACTTTCAGCGCCAAGAAAAAGGTCGACGATTTCACAAGCGATAATGGGTTTGTGAGGACGCCGCAGAACGGTGAGTTTCTGACGGAGATGGAGGAGAAGAGTACCGACGTCGTCATTGTCGGCGCCGGAGTTGCCGGTGCTGCTCTTGCTTACACTCTTGCCAAGGAAGGACGGCGTGTGCATGTCATTGAAAGAGATTTGAGTGAGCCAGACAGAATTGTTGGTGAGCTTTTGCAGCCTGGGGGTTATCTTAAATTGATTGAGTTGGGTCTTCAGGACTGTGCAAACGAGTCAATTGATGCTCAGAAGGTCTTTGGTTATGCTCTTTACAAAGATGGCAAGGATACAAAGCTGTCTTATCCTTTGGAGCAGTATAATTCAGATGTTGCGGGGAGAAGTTTCCACAATGGGCGTTTTATTCAAAAAATGCGTGAAAAAGCTGTCACTCTTTCAAATGTCAAATTGGAACAAGGATCAGTGACAACACTAATCGAGGAAAAGGGCATTGTTAAAGGGGTGATGTACAAGAACAAGGCTGGAGAGGAGATGAGAGCATATGCTCCACTGACAATTGTGTGTGATGGCTGCTTTTCAAATCTGCGAAAATCTCTCAGTGCTCCACAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGGTTTTAGAGAACTGTGAGCTTCCACACGCAAATCATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTGTTTTATCCTATTAGTTCCACTGAGGTTCGTTGCTTGGTCGATGTACCTGGAACAAAAGTACCCTCAGTAGCTAATGGTGAAATGGCCAAGTATTTGAAGACTGTGGTGGCTCCTCAGATTCCCCCTCAGCTCCTCAATGCTTTTATGGCTGCCATAGACAAGGGAAACATTAGGTCCATGCAAAACAGAAGCATGGCTGCTAATCCGGTTCCCACTCCTGGTGCACTTTTGTTAGGCGATTCATTCAACATGAGGCATCCTTTAACAGGAGGAGGAATGACTGTGGCTCTTTCAGACATTGTTCTTCTCCGTGATCTTCTTAGACCCCTACGTGATCTTAATGATGCACCTGCTTTGTGCAATTACCTTGAATCATTTTATACATTGCGTAAGCCTGTGTCATCTACTATAAACACATTGGCAGGTGCTTTGTATAAGGTGTTTTTAGCATCACCTGATCCAGCGAGACAGGAAATGCGTGAAGCATGTTTTGGCTATTTGAGCCTTGGAGGAATCTGTTCATATGGACCAGTATCACTGCTCTCTGGCCTTAATCCCCGTCCACTGCACTTGTTCCTCCATTTCTTTGCTGTTGCTATCTACGGTGTTGGACGCTTAATGTTTCCATTCCCTTCACCACAGCGCATGTGGCTTGGGTTTAGATTAATCATGAGTGCATCAGGCATCATATTCCCCATTATTAAGGGCGAAGGAGTTAGACAGATGTTCTTTCCAGCAACAGTGCCAGCATATTATAGATCAGCACCAGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

527

Amino Acids

57.43

Weight (kDa)

8.24

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 61 - 372 2.8e-18 FAD binding domain
DAO PF01266 62 - 92 4.9e-08 FAD dependent oxidoreductase
FAD_binding_2 PF00890 62 - 91 1e-05 FAD binding domain
NAD_binding_8 PF13450 65 - 91 2.1e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 213 - 485 1.1e-117 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1162, 1217
AatII GACGTC 1 cut(s) 189
Acc36I ACCTGC 2 cut(s) 1162, 1217
AccB1I GGYRCC 1 cut(s) 200
AccI GTMKAC 1 cut(s) 102
AciI CCGC 3 cut(s) 27, 140, 467
AclWI GGATC 2 cut(s) 559, 1256
AcoI YGGCCR 1 cut(s) 888
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 3 cut(s) 137, 186, 201
AfaI GTAC 5 cut(s) 17, 181, 602, 849, 864
AfiI CCNNNNNNNGG 3 cut(s) 146, 1022, 1070
AflIII ACRYGT 1 cut(s) 772
AgsI TTSAA 8 cut(s) 269, 500, 531, 672, 707, 901, 1051, 1175
AjnI CCWGG 4 cut(s) 33, 316, 850, 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 1278, 1310
AluBI AGCT 6 cut(s) 307, 430, 518, 751, 875, 934
AluI AGCT 6 cut(s) 307, 430, 518, 751, 875, 934
Alw21I GWGCWC 2 cut(s) 697, 1030
Alw26I GTCTC 2 cut(s) 780, 1264
Alw44I GTGCAC 1 cut(s) 1026
AlwI GGATC 2 cut(s) 559, 1256
AoxI GGCC 2 cut(s) 888, 1351
ApaLI GTGCAC 1 cut(s) 1026
ApeKI GCWGC 5 cut(s) 218, 313, 663, 953, 1001
AseI ATTAAT 1 cut(s) 1469
Asp700I GAANNNNTTC 1 cut(s) 1321
AspLEI GCGC 3 cut(s) 89, 203, 1449
AspS9I GGNCC 2 cut(s) 978, 1331
AsuHPI GGTGA 8 cut(s) 64, 161, 314, 607, 786, 893, 1248, 1431
AvaII GGWCC 2 cut(s) 978, 1331
BaeGI GKGCMC 1 cut(s) 1030
BaeI ACNNNNGTAYC 2 cut(s) 846, 879
BalI TGGCCA 1 cut(s) 890
BanI GGYRCC 1 cut(s) 200
BbsI GAAGAC 2 cut(s) 341, 908
Bbv12I GWGCWC 2 cut(s) 697, 1030
BbvCI CCTCAGC 1 cut(s) 930
BbvI GCAGC 5 cut(s) 205, 325, 650, 940, 988
BccI CCATC 4 cut(s) 160, 407, 653, 806
BceAI ACGGC 1 cut(s) 266
BciT130I CCWGG 4 cut(s) 35, 318, 852, 1023
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 780, 1264
BfoI RGCGCY 2 cut(s) 90, 204
BfuAI ACCTGC 2 cut(s) 1162, 1217
BfuI GTATCC 1 cut(s) 415
BisI GCNGC 6 cut(s) 140, 219, 314, 664, 954, 1002
BlsI GCNGC 6 cut(s) 141, 220, 315, 665, 955, 1003
Bme1390I CCNGG 4 cut(s) 35, 318, 852, 1023
Bme18I GGWCC 2 cut(s) 978, 1331
BmgT120I GGNCC 2 cut(s) 978, 1331
BmiI GGNNCC 3 cut(s) 202, 915, 1014
BmrFI CCNGG 4 cut(s) 35, 318, 852, 1023
BmsI GCATC 6 cut(s) 367, 1069, 1138, 1262, 1490, 1497
BpiI GAAGAC 2 cut(s) 341, 908
BpmI CTGGAG 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 930
BsaAI YACGTR 1 cut(s) 1136
BsaHI GRCGYC 3 cut(s) 137, 186, 201
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 240, 317, 1309
BsaWI WCCGGW 1 cut(s) 1009
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 1022, 1070
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 1334, 1571
Bse3DI GCAATG 1 cut(s) 1187
BseBI CCWGG 4 cut(s) 35, 318, 852, 1023
BseDI CCNNGG 3 cut(s) 240, 317, 1309
BseGI GGATG 2 cut(s) 798, 1060
BseLI CCNNNNNNNGG 3 cut(s) 146, 1022, 1070
BseMI GCAATG 1 cut(s) 1187
BseMII CTCAG 6 cut(s) 395, 703, 816, 882, 932, 944
BseNI ACTGG 2 cut(s) 1334, 1571
BseRI GAGGAG 5 cut(s) 185, 635, 906, 926, 1089
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 5 cut(s) 205, 325, 650, 940, 988
BsgI GTGCAG 1 cut(s) 1355
BshFI GGCC 2 cut(s) 890, 1353
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 2 cut(s) 697, 1030
BsiSI CCGG 3 cut(s) 204, 213, 1010
BslFI GGGAC 1 cut(s) 696
BslI CCNNNNNNNGG 3 cut(s) 146, 1022, 1070
BsmAI GTCTC 2 cut(s) 780, 1264
BsmFI GGGAC 1 cut(s) 696
BsnI GGCC 2 cut(s) 890, 1353
Bso31I GGTCTC 1 cut(s) 780
Bsp1286I GDGCHC 2 cut(s) 697, 1030
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 6 cut(s) 66, 551, 1117, 1138, 1261, 1562
BspACI CCGC 3 cut(s) 27, 140, 467
BspANI GGCC 2 cut(s) 890, 1353
BspCNI CTCAG 6 cut(s) 394, 702, 817, 881, 931, 943
BspHI TCATGA 2 cut(s) 63, 1473
BspLI GGNNCC 3 cut(s) 202, 915, 1014
BspMI ACCTGC 2 cut(s) 1162, 1217
BspPI GGATC 2 cut(s) 559, 1256
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 780
BsrDI GCAATG 1 cut(s) 1187
BsrFI RCCGGY 1 cut(s) 212
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 2 cut(s) 1334, 1571
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 3 cut(s) 240, 317, 1309
BssMI GATC 6 cut(s) 66, 551, 1117, 1138, 1261, 1562
BssNI GRCGYC 3 cut(s) 137, 186, 201
BssT1I CCWWGG 2 cut(s) 240, 1309
Bst2UI CCWGG 4 cut(s) 35, 318, 852, 1023
Bst4CI ACNGT 7 cut(s) 149, 359, 746, 907, 1087, 1409, 1546
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 3 cut(s) 137, 186, 201
BstAUI TGTACA 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 1136
BstC8I GCNNGC 1 cut(s) 1551
BstDEI CTNAG 7 cut(s) 381, 689, 825, 868, 918, 930, 1124
BstENI CCTNNNNNAGG 1 cut(s) 1068
BstF5I GGATG 2 cut(s) 798, 1060
BstH2I RGCGCY 2 cut(s) 90, 204
BstHHI GCGC 3 cut(s) 89, 203, 1449
BstKTI GATC 6 cut(s) 69, 554, 1120, 1141, 1264, 1565
BstMAI GTCTC 2 cut(s) 780, 1264
BstMBI GATC 6 cut(s) 66, 551, 1117, 1138, 1261, 1562
BstMWI GCNNNNNNNGC 7 cut(s) 209, 218, 224, 313, 950, 1305, 1453
BstNI CCWGG 4 cut(s) 35, 318, 852, 1023
BstNSI RCATGY 4 cut(s) 263, 776, 1293, 1453
BstSCI CCNGG 4 cut(s) 33, 316, 850, 1021
BstSLI GKGCMC 1 cut(s) 1030
BstV1I GCAGC 5 cut(s) 205, 325, 650, 940, 988
BstV2I GAAGAC 2 cut(s) 341, 908
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 2 cut(s) 890, 1353
BtsCI GGATG 2 cut(s) 798, 1060
BtsI GCAGTG 2 cut(s) 1340, 1367
BtsIMutI CAGTG 7 cut(s) 561, 641, 697, 822, 1340, 1367, 1551
BveI ACCTGC 2 cut(s) 1162, 1217
Cac8I GCNNGC 1 cut(s) 1551
CciI TCATGA 2 cut(s) 63, 1473
CfoI GCGC 3 cut(s) 89, 203, 1449
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 978, 1331
CseI GACGC 2 cut(s) 145, 1425
Csp6I GTAC 5 cut(s) 16, 180, 601, 848, 863
CspCI CAANNNNNGTGG 2 cut(s) 1356, 1391
CviQI GTAC 5 cut(s) 16, 180, 601, 848, 863
DdeI CTNAG 7 cut(s) 381, 689, 825, 868, 918, 930, 1124
DinI GGCGCC 1 cut(s) 202
DpnI GATC 6 cut(s) 68, 553, 1119, 1140, 1263, 1564
DpnII GATC 6 cut(s) 66, 551, 1117, 1138, 1261, 1562
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
EciI GGCGGA 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 240, 1309
Eco31I GGTCTC 1 cut(s) 780
Eco47I GGWCC 2 cut(s) 978, 1331
Eco57I CTGAAG 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 1068
EcoRII CCWGG 4 cut(s) 33, 316, 850, 1021
EcoT14I CCWWGG 2 cut(s) 240, 1309
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 2 cut(s) 240, 1309
FalI AAGNNNNNCTT 2 cut(s) 700, 732
FaqI GGGAC 1 cut(s) 696
FauI CCCGC 1 cut(s) 460
FauNDI CATATG 2 cut(s) 634, 1327
FbaI TGATCA 1 cut(s) 66
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 6 cut(s) 140, 219, 314, 664, 954, 1002
FokI GGATG 2 cut(s) 785, 1047
Fsp4HI GCNGC 6 cut(s) 140, 219, 314, 664, 954, 1002
GlaI GCGC 3 cut(s) 88, 202, 1448
GluI GCNGC 6 cut(s) 140, 219, 314, 664, 954, 1002
GsuI CTGGAG 1 cut(s) 636
HaeII RGCGCY 2 cut(s) 90, 204
HaeIII GGCC 2 cut(s) 890, 1353
HapII CCGG 3 cut(s) 204, 213, 1010
HgaI GACGC 2 cut(s) 145, 1425
HhaI GCGC 3 cut(s) 89, 203, 1449
Hin1I GRCGYC 3 cut(s) 137, 186, 201
Hin6I GCGC 3 cut(s) 87, 201, 1447
HinP1I GCGC 3 cut(s) 87, 201, 1447
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 6 cut(s) 30, 368, 922, 1043, 1175, 1317
HpaII CCGG 3 cut(s) 204, 213, 1010
HphI GGTGA 8 cut(s) 64, 161, 314, 607, 786, 893, 1248, 1431
Hpy166II GTNNAC 3 cut(s) 103, 1028, 1367
Hpy188I TCNGA 5 cut(s) 159, 384, 460, 921, 1099
Hpy188III TCNNGA 3 cut(s) 64, 353, 1474
Hpy8I GTNNAC 3 cut(s) 103, 1028, 1367
Hpy99I CGWCG 3 cut(s) 107, 188, 191
HpyAV CCTTC 5 cut(s) 239, 379, 801, 1446, 1508
HpyCH4III ACNGT 7 cut(s) 149, 359, 746, 907, 1087, 1409, 1546
HpyCH4IV ACGT 3 cut(s) 18, 186, 1135
HpyCH4V TGCA 9 cut(s) 259, 313, 362, 985, 1028, 1151, 1164, 1372, 1481
HpyF10VI GCNNNNNNNGC 7 cut(s) 209, 218, 224, 313, 950, 1305, 1453
HpyF3I CTNAG 7 cut(s) 381, 689, 825, 868, 918, 930, 1124
HpySE526I ACGT 3 cut(s) 18, 186, 1135
Hsp92I GRCGYC 3 cut(s) 137, 186, 201
HspAI GCGC 3 cut(s) 87, 201, 1447
KasI GGCGCC 1 cut(s) 200
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 6 cut(s) 66, 551, 1117, 1138, 1261, 1562
LmnI GCTCC 5 cut(s) 445, 643, 700, 919, 939
Lsp1109I GCAGC 5 cut(s) 205, 325, 650, 940, 988
LweI GCATC 6 cut(s) 367, 1069, 1138, 1262, 1490, 1497
MaeII ACGT 3 cut(s) 18, 186, 1135
MaeIII GTNAC 2 cut(s) 520, 556
MalI GATC 6 cut(s) 68, 553, 1119, 1140, 1263, 1564
MboI GATC 6 cut(s) 66, 551, 1117, 1138, 1261, 1562
MboII GAAGA 6 cut(s) 52, 187, 341, 913, 1100, 1112
MfeI CAATTG 2 cut(s) 372, 648
MhlI GDGCHC 2 cut(s) 697, 1030
MlsI TGGCCA 1 cut(s) 890
MluCI AATT 7 cut(s) 294, 332, 372, 454, 539, 648, 1165
MluNI TGGCCA 1 cut(s) 890
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 2 cut(s) 39, 377
MmeI TCCRAC 1 cut(s) 1393
Mox20I TGGCCA 1 cut(s) 890
MroXI GAANNNNTTC 1 cut(s) 1321
MscI TGGCCA 1 cut(s) 890
MseI TTAA 8 cut(s) 330, 588, 1067, 1143, 1356, 1421, 1469, 1506
MslI CAYNNNNRTG 1 cut(s) 771
Msp20I TGGCCA 1 cut(s) 890
MspI CCGG 3 cut(s) 204, 213, 1010
MspR9I CCNGG 4 cut(s) 35, 318, 852, 1023
MunI CAATTG 2 cut(s) 372, 648
MvaI CCWGG 4 cut(s) 35, 318, 852, 1023
MwoI GCNNNNNNNGC 7 cut(s) 209, 218, 224, 313, 950, 1305, 1453
NarI GGCGCC 1 cut(s) 201
NdeI CATATG 2 cut(s) 634, 1327
NdeII GATC 6 cut(s) 66, 551, 1117, 1138, 1261, 1562
NlaIV GGNNCC 3 cut(s) 202, 915, 1014
NmuCI GTSAC 2 cut(s) 520, 556
NspI RCATGY 4 cut(s) 263, 776, 1293, 1453
PagI TCATGA 2 cut(s) 63, 1473
PaqCI CACCTGC 2 cut(s) 1162, 1217
PciI ACATGT 1 cut(s) 772
PdmI GAANNNNTTC 1 cut(s) 1321
PfeI GAWTC 4 cut(s) 922, 1043, 1175, 1317
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 6 cut(s) 141, 220, 315, 665, 955, 1003
PleI GAGTC 2 cut(s) 38, 376
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 2 cut(s) 38, 376
Ppu21I YACGTR 1 cut(s) 1136
PscI ACATGT 1 cut(s) 772
PshBI ATTAAT 1 cut(s) 1469
Psp6I CCWGG 4 cut(s) 33, 316, 850, 1021
PspGI CCWGG 4 cut(s) 33, 316, 850, 1021
PspN4I GGNNCC 3 cut(s) 202, 915, 1014
PspPI GGNCC 2 cut(s) 978, 1331
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 5 cut(s) 17, 181, 602, 849, 864
RsaNI GTAC 5 cut(s) 16, 180, 601, 848, 863
RseI CAYNNNNRTG 1 cut(s) 771
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 8 cut(s) 330, 588, 1067, 1143, 1356, 1421, 1469, 1506
SatI GCNGC 6 cut(s) 140, 219, 314, 664, 954, 1002
Sau3AI GATC 6 cut(s) 66, 551, 1117, 1138, 1261, 1562
Sau96I GGNCC 2 cut(s) 978, 1331
SchI GAGTC 2 cut(s) 39, 377
ScrFI CCNGG 4 cut(s) 35, 318, 852, 1023
SduI GDGCHC 2 cut(s) 697, 1030
SfaNI GCATC 6 cut(s) 367, 1069, 1138, 1262, 1490, 1497
SfoI GGCGCC 1 cut(s) 202
SinI GGWCC 2 cut(s) 978, 1331
SmiMI CAYNNNNRTG 1 cut(s) 771
Sse9I AATT 7 cut(s) 294, 332, 372, 454, 539, 648, 1165
SsiI CCGC 3 cut(s) 27, 140, 467
SspDI GGCGCC 1 cut(s) 200
StyD4I CCNGG 4 cut(s) 33, 316, 850, 1021
StyI CCWWGG 2 cut(s) 240, 1309
TaaI ACNGT 7 cut(s) 149, 359, 746, 907, 1087, 1409, 1546
TaiI ACGT 3 cut(s) 21, 189, 1138
TaqI TCGA 3 cut(s) 102, 570, 843
TasI AATT 7 cut(s) 294, 332, 372, 454, 539, 648, 1165
TatI WGTACW 1 cut(s) 600
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 4 cut(s) 922, 1043, 1175, 1317
Tru1I TTAA 8 cut(s) 330, 588, 1067, 1143, 1356, 1421, 1469, 1506
Tru9I TTAA 8 cut(s) 330, 588, 1067, 1143, 1356, 1421, 1469, 1506
TscAI CASTG 7 cut(s) 561, 648, 697, 829, 1347, 1374, 1551
TseFI GTSAC 2 cut(s) 520, 556
TseI GCWGC 5 cut(s) 218, 313, 663, 953, 1001
Tsp45I GTSAC 2 cut(s) 520, 556
TspDTI ATGAA 4 cut(s) 52, 1035, 1314, 1565
TspGWI ACGGA 2 cut(s) 176, 1103
TspRI CASTG 7 cut(s) 561, 648, 697, 829, 1347, 1374, 1551
Tth111I GACNNNGTC 1 cut(s) 188
VneI GTGCAC 1 cut(s) 1026
VpaK11BI GGWCC 2 cut(s) 978, 1331
VspI ATTAAT 1 cut(s) 1469
XagI CCTNNNNNAGG 1 cut(s) 1068
XceI RCATGY 4 cut(s) 263, 776, 1293, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 1449
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 1321
ZraI GACGTC 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.