RLG00000015247

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000108
Physical Location & Seq
Reverse (-)
63560054 .. 63569957
9904 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015247

Sequence Viewer

Length: 3309 bp
ATGTCAAGCGCCTCACGCGCCTGGTTTGGGAATTTTCGAGCTCTACTAGGGCTCTGGGATAAGATTTGGAGTAATCATGATCTGTCCTCTACTTTTGAAGATTTTAAGAACCTAATTGTGCTTTTCCATTTGCACTTTAATTGGGAAGTGGAGAAGCCGCCTCTTTGGATTTTGTCTACAGATTCGAACAGAACCAATGATGATTTGAAAGCTTCTCCCCCCTTGACGGCCAACCAAGCCAATTGGTCAGTTCATTTCAGAGCCCTCTCTTTGGTTTTCGCCAGATTCGTGATTACTTGGTATGTTGAATTTTTGCGTCTCCAGCCCCTGGATTCTGTTTTGCCTCAATCAAGCCTTCTCTCTAATCCTTTCCTTCAATCCTTTATTCAGCTTACTGTCTTCACTCACCCAATGGCTTTTAGTCCAAATGCGAGGGCTCAAGCCAGCTCTTCGCGAAACCCTTTCAGCTTTTGGCAAATTCCCTTTCCCCGGCCTAGAGCTAATGAACCCCCTCTTAGCATCAACATTGGTGATACTAAGGTTCGGGTGGATCAGAAAGAAACCCATGTCACCATCAAACGACCCCCTTCTGATTATGCTGAGCACTACCGCCGGTATTGTTTGGTTGGAAGAATCTTCGGGAAACAGCTTGATGCAGATACTGTGATGCAAAAGGCTGCTCTTCTCTGGAGGGACGTTCAAGGCTCCCTCAATCTAAACAAACTAGCCAGCAACTGGTTCAGACTTCAATTCTCTCTCGAAGATGATCTTCTTTATGTTCTCGATGAGCGCCCTTGGTACATCAAAGGGAGAATCTTTCATGTCCAGCGTTGGTTTCCTGGTTTTGATCCTCTCTTCACCAGGATTCAGCATTTGGTGGTTTGGATTCGCCTCCCAAATCTTCCTCTCCACTATTATGACCCCAAAGTCCTTGAGGATGCTTATGGCAGCCACAAACACAAAAACAATACCTGTCCTTTCAGAGTAAGAGAAGAAGATTTTATCCTAGTTGACCGCTTTTCAGATGATGATGAAGTTGAGCCCTCTGAGAAAGAACTTGCTCCTGAAATTAAAGCCTTACTTTCTGACAAAGTTATGGCAGTTTTTCCTCAACCCAATACCTCTGTTATTCGTGGTCAAGGCCCCACTAAATCCTTTGCTGAACAAGAAGGCCAGGATGATATTAATCCCCTATCTCCAAACTGGACCCTTGTGACCCGGAAAAGGACTCTAGGTGCCAAGTCCTTTACTGGAAAACAAGGTAATGGCAGATCCACTATTGGAACCAGATCCCTACCAACTCCAAACCGGAGTGGTTTAGTCAAAGATGGCCTCTCTTTCAAAGACGTTACTCGGGGTGGTATCACTATTGGTGGCCCACTTAGGACTGTGAATAAGATTGGAAATTCTGCTGCTGATCTTTCTCCCAACTCCCAGGACTTAATGAGTGGTTCTTTACACTTTAATGATCCTAAGCCTCTTCTTTCTTTCTTTGCATTCCCTAATATTGCTAAACTCTATGCCTCTCCTACTGTTGACAACTACATTGACAACTTTATGGATGCCAAAGTGGATGATGAAAATGCTTTCATCAATAATCTAATTGCACCTGAAAATTCTGCCGTGGAGGATTGGGATACCGACAACCTGGAAGATCACATGGAAGTTAGCTCTGGATATCGCAACCCACTGAAACAATCCAGAGACATGATGGATGATATGCCTAGTGAGAATGACAAAAACAAGGAGGCTGGTCCCTCCCAATTTAATAAAATGGTGTCTTACGAGTACGTTCTGGGTGGAGTCTTGGCTTCTCTGCTAGGCTCTGTTTTCCTCATGATCATCAGCAGCACTTTCAGCGCGAAGAAAAAGGTGGAGGATTTGAAAAGTAATAATGGGTTTGTGAGGATGCCGCAGAACGGTGCTTTTCGGCCGGAGATGGAGGAGGAGGAGAAAAGTACCGACGTCGTCATTGTCGGCGCCGGAGTTGCCGGTGCAGCTCTTGCTTACACTCTTGCCAAGGAAGGACGGCGTGTACATGTTATTGAAAGAGACTTGAGTGAGCCAGACAGAATTGTTGGTGAGCTTTTGCAGCCTGGGGGTTATCTCAAATTGATTGAGTTGGGTCTTGAAGATTGTGCAAACGAGTCAATTGATGCTCAGAAGGTCTTTGGTTATGCTCTTTACAAAGATGGCAGGGATACAAAACTATCTTATCCCTTGGAGAAGTTTCCACAATGGGCGTTTTATTCAAAGAATGCTGTCAAATTGGAACAAGGATCGGTGACAACACTAATCGAGGAAAAGGGCATTGTCAAAGGGGTGATTTACAAGAACAAGGCTGGAGAGGAGATGAGAACATATGCTCCACTAACAATTGTGTGCGATGGCTGCTTTTCAAATCTGCGCAAATCTCTCAGTGCTCCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGGTGTTAGAGAACTGTGATCTTCCACATGCAAATCATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTGTTTTATCCTATTAGTTCCACTGAGATTCGTTGTTTGGTTGATGTACCTGGGACAAAAGTACCTTCAGTTGCTAGTGGTGAAATGGCTAACTATTTGAAGACTGTGGTGGCCCCTCAGGTTCCCCCACGGCTGTACAATGCTTTTATGGTTGCAATAGAGAAAGGAAACATTAGATCCATGCAAAACAAAAGCATGGCTGCTAATCCTATTCCCACTCCTGGTGCAATTTTGTTGGGTGATTCATTCAATATGAGGCATCCTTTAACAGGAGGAGGGATGACCGTGGCTTTTTCAGACATAGTTGTTCTCCGCGATCTTCTTAGACCCCTACGTGATCTTAATGATGCACCTGCTTTGTGCAATTACCTTGAATCATTTTATACATTGCGTAAGCCTGTGTCATCTACTATAAACACATTGGCAGGTGCTTTGTACAAGGTGTTTTGTGCATCGCCTGATCCAGCAAGACAGGAAATGTGTGAAGCATGTTTTGGCTATTTGAGCCTTGGAGGAATCTGTTCATATGGACCAGTATCTCTGCTCTCTGGCCTTAACCCTCGTCCGCTACACTTGTTCCTCCATTTCTTTGCTGTTGCTATCTATGGTGTTGGGCGCTTAATGCTTCCATTCCCTTCACCTCAACGCATCTGGCTTGGGTTTAGATTGATCTTAAGTACATCAGGCATCATATTCCCCATTATAAAGGGAGAAGGAGTTAGACAGATGTTCTTTCCAGCAACGGTGCCAGCATATTATAGATCACCACCTGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

1103

Amino Acids

123.27

Weight (kDa)

6.9

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 205 - 284 4.8e-12 Domain of unknown function (DUF4283)
FAD_binding_3 PF01494 654 - 948 4.8e-14 FAD binding domain
DAO PF01266 655 - 685 1.1e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 658 - 684 4.8e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 789 - 1061 2.8e-115 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3233
AarI CACCTGC 2 cut(s) 2890, 2945
AasI GACNNNNNNGTC 1 cut(s) 926
AatII GACGTC 1 cut(s) 1968
Acc16I TGCGCA 1 cut(s) 2408
Acc36I ACCTGC 2 cut(s) 2890, 2945
AccB1I GGYRCC 3 cut(s) 1235, 1979, 3274
AccB7I CCANNNNNTGG 2 cut(s) 328, 735
AccI GTMKAC 1 cut(s) 176
AccII CGCG 4 cut(s) 18, 454, 1862, 2844
AciI CCGC 6 cut(s) 158, 610, 1015, 1913, 2842, 3095
AclWI GGATC 8 cut(s) 558, 842, 1266, 1284, 1463, 2287, 2700, 2984
AcoI YGGCCR 2 cut(s) 228, 1931
AcsI RAATTY 5 cut(s) 31, 308, 477, 1405, 1615
AcuI CTGAAG 1 cut(s) 2580
AcyI GRCGYC 2 cut(s) 1965, 1980
AfaI GTAC 9 cut(s) 800, 1790, 1960, 2037, 2577, 2592, 2666, 2966, 3208
AflII CTTAAG 1 cut(s) 3202
AflIII ACRYGT 2 cut(s) 2038, 2500
AjuI GAANNNNNNNTTGG 2 cut(s) 3006, 3038
AloI GAACNNNNNNTCC 2 cut(s) 2350, 2382
Alw21I GWGCWC 3 cut(s) 43, 606, 2425
Alw26I GTCTC 4 cut(s) 323, 1698, 2046, 2508
AlwI GGATC 8 cut(s) 558, 842, 1266, 1284, 1463, 2287, 2700, 2984
AlwNI CAGNNNCTG 3 cut(s) 328, 662, 735
Ama87I CYCGRG 1 cut(s) 1353
AoxI GGCC 9 cut(s) 228, 491, 1141, 1171, 1330, 1375, 1931, 2640, 3079
ApeKI GCWGC 8 cut(s) 677, 948, 1412, 1848, 1997, 2092, 2391, 2729
ApoI RAATTY 5 cut(s) 31, 308, 477, 1405, 1615
AseI ATTAAT 1 cut(s) 1185
Asp700I GAANNNNTTC 1 cut(s) 3049
AspLEI GCGC 7 cut(s) 11, 20, 792, 1862, 1982, 2409, 3147
AspS9I GGNCC 6 cut(s) 1142, 1206, 1376, 1754, 2641, 3059
AsuC2I CCSGG 2 cut(s) 490, 1219
AsuII TTCGAA 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 1353
AvaII GGWCC 3 cut(s) 1206, 1754, 3059
AxyI CCTNAGG 1 cut(s) 2646
BaeI ACNNNNGTAYC 4 cut(s) 525, 558, 2574, 2607
BanI GGYRCC 3 cut(s) 1235, 1979, 3274
BanII GRGCYC 5 cut(s) 43, 54, 265, 439, 1044
BbsI GAAGAC 2 cut(s) 391, 2636
Bbv12I GWGCWC 3 cut(s) 43, 606, 2425
BbvI GCAGC 8 cut(s) 664, 960, 1399, 1860, 2009, 2104, 2378, 2716
BccI CCATC 7 cut(s) 581, 1322, 1705, 1933, 2186, 2381, 2534
BceAI ACGGC 4 cut(s) 243, 1607, 2045, 2675
BciVI GTATCC 2 cut(s) 1630, 2194
BclI TGATCA 1 cut(s) 1839
BcnI CCSGG 2 cut(s) 490, 1219
BcoDI GTCTC 4 cut(s) 323, 1698, 2046, 2508
BfaI CTAG 8 cut(s) 47, 495, 725, 1007, 1232, 1725, 1820, 2604
BfmI CTRYAG 1 cut(s) 177
BfoI RGCGCY 4 cut(s) 12, 793, 1983, 3148
BfrI CTTAAG 1 cut(s) 3202
BfuAI ACCTGC 2 cut(s) 2890, 2945
BfuI GTATCC 2 cut(s) 1630, 2194
BlpI GCTNAGC 1 cut(s) 600
Bme18I GGWCC 3 cut(s) 1206, 1754, 3059
BmeT110I CYCGRG 1 cut(s) 1353
BmgT120I GGNCC 6 cut(s) 1142, 1206, 1376, 1754, 2641, 3059
BpiI GAAGAC 2 cut(s) 391, 2636
BpmI CTGGAG 3 cut(s) 305, 709, 2364
Bpu10I CCTNAGC 1 cut(s) 1473
Bpu1102I GCTNAGC 1 cut(s) 600
Bpu14I TTCGAA 1 cut(s) 185
BpuEI CTTGAG 3 cut(s) 423, 953, 2077
BpuMI CCSGG 2 cut(s) 490, 1219
BsaAI YACGTR 1 cut(s) 2864
BsaBI GATNNNNATC 1 cut(s) 1185
BsaHI GRCGYC 2 cut(s) 1965, 1980
BsaI GGTCTC 1 cut(s) 2508
BsaWI WCCGGW 1 cut(s) 1308
BsaXI ACNNNNNCTCC 4 cut(s) 2350, 2380, 3231, 3261
Bse118I RCCGGY 2 cut(s) 612, 1991
Bse1I ACTGG 4 cut(s) 740, 1208, 1255, 3062
Bse21I CCTNAGG 1 cut(s) 2646
Bse3DI GCAATG 1 cut(s) 2915
Bse8I GATNNNNATC 1 cut(s) 1185
BseGI GGATG 9 cut(s) 943, 1183, 1567, 1579, 1720, 1914, 2526, 2788, 2814
BseJI GATNNNNATC 1 cut(s) 1185
BseMI GCAATG 1 cut(s) 2915
BseMII CTCAG 6 cut(s) 591, 1038, 2174, 2431, 2544, 2660
BseNI ACTGG 4 cut(s) 740, 1208, 1255, 3062
BseRI GAGGAG 5 cut(s) 1958, 1961, 1964, 2363, 2817
BseX3I CGGCCG 1 cut(s) 1931
BseXI GCAGC 8 cut(s) 664, 960, 1399, 1860, 2009, 2104, 2378, 2716
BsgI GTGCAG 1 cut(s) 2016
Bsh1236I CGCG 4 cut(s) 18, 454, 1862, 2844
Bsh1285I CGRYCG 1 cut(s) 1934
BshFI GGCC 9 cut(s) 230, 493, 1143, 1173, 1332, 1377, 1933, 2642, 3081
BshNI GGYRCC 3 cut(s) 1235, 1979, 3274
BsiEI CGRYCG 1 cut(s) 1934
BsiHKAI GWGCWC 3 cut(s) 43, 606, 2425
BsiHKCI CYCGRG 1 cut(s) 1353
BsiSI CCGG 7 cut(s) 490, 613, 1219, 1309, 1934, 1983, 1992
BslFI GGGAC 4 cut(s) 707, 1740, 2424, 2596
BsmAI GTCTC 4 cut(s) 323, 1698, 2046, 2508
BsmBI CGTCTC 1 cut(s) 323
BsmFI GGGAC 4 cut(s) 707, 1740, 2424, 2596
BsmI GAATGC 2 cut(s) 1496, 2263
BsnI GGCC 9 cut(s) 230, 493, 1143, 1173, 1332, 1377, 1933, 2642, 3081
Bso31I GGTCTC 1 cut(s) 2508
BsoBI CYCGRG 1 cut(s) 1353
Bsp119I TTCGAA 1 cut(s) 185
Bsp1286I GDGCHC 7 cut(s) 43, 54, 265, 439, 606, 1044, 2425
Bsp1407I TGTACA 3 cut(s) 2035, 2664, 2964
Bsp1720I GCTNAGC 1 cut(s) 600
Bsp68I TCGCGA 1 cut(s) 454
BspACI CCGC 6 cut(s) 158, 610, 1015, 1913, 2842, 3095
BspANI GGCC 9 cut(s) 230, 493, 1143, 1173, 1332, 1377, 1933, 2642, 3081
BspCNI CTCAG 6 cut(s) 592, 1039, 2173, 2430, 2545, 2659
BspFNI CGCG 4 cut(s) 18, 454, 1862, 2844
BspHI TCATGA 2 cut(s) 76, 1836
BspMI ACCTGC 2 cut(s) 2890, 2945
BspPI GGATC 8 cut(s) 558, 842, 1266, 1284, 1463, 2287, 2700, 2984
BspQI GCTCTTC 2 cut(s) 454, 687
BspT104I TTCGAA 1 cut(s) 185
BspT107I GGYRCC 3 cut(s) 1235, 1979, 3274
BspTI CTTAAG 1 cut(s) 3202
BspTNI GGTCTC 1 cut(s) 2508
BsrDI GCAATG 1 cut(s) 2915
BsrFI RCCGGY 2 cut(s) 612, 1991
BsrGI TGTACA 3 cut(s) 2035, 2664, 2964
BsrI ACTGG 4 cut(s) 740, 1208, 1255, 3062
BssAI RCCGGY 2 cut(s) 612, 1991
BssNI GRCGYC 2 cut(s) 1965, 1980
BssT1I CCWWGG 4 cut(s) 794, 2019, 2220, 3037
Bst4CI ACNGT 9 cut(s) 397, 664, 1390, 1534, 1922, 2474, 2635, 2815, 3274
Bst6I CTCTTC 4 cut(s) 454, 687, 860, 1485
BstACI GRCGYC 2 cut(s) 1965, 1980
BstAFI CTTAAG 1 cut(s) 3202
BstAPI GCANNNNNTGC 1 cut(s) 2003
BstAUI TGTACA 3 cut(s) 2035, 2664, 2964
BstBAI YACGTR 1 cut(s) 2864
BstBI TTCGAA 1 cut(s) 185
BstC8I GCNNGC 3 cut(s) 445, 730, 3279
BstDSI CCRYGG 3 cut(s) 1623, 2657, 2814
BstENI CCTNNNNNAGG 1 cut(s) 2796
BstF5I GGATG 9 cut(s) 943, 1183, 1567, 1579, 1720, 1914, 2526, 2788, 2814
BstFNI CGCG 4 cut(s) 18, 454, 1862, 2844
BstH2I RGCGCY 4 cut(s) 12, 793, 1983, 3148
BstHHI GCGC 7 cut(s) 11, 20, 792, 1862, 1982, 2409, 3147
BstMAI GTCTC 4 cut(s) 323, 1698, 2046, 2508
BstMCI CGRYCG 1 cut(s) 1934
BstNSI RCATGY 4 cut(s) 2042, 2489, 2504, 3021
BstSFI CTRYAG 1 cut(s) 177
BstUI CGCG 4 cut(s) 18, 454, 1862, 2844
BstV1I GCAGC 8 cut(s) 664, 960, 1399, 1860, 2009, 2104, 2378, 2716
BstV2I GAAGAC 2 cut(s) 391, 2636
BstX2I RGATCY 3 cut(s) 1271, 1289, 2705
BstYI RGATCY 3 cut(s) 1271, 1289, 2705
BstZI CGGCCG 1 cut(s) 1931
Bsu36I CCTNAGG 1 cut(s) 2646
BsuI GTATCC 2 cut(s) 1630, 2194
BsuRI GGCC 9 cut(s) 230, 493, 1143, 1173, 1332, 1377, 1933, 2642, 3081
BtgI CCRYGG 3 cut(s) 1623, 2657, 2814
BtgZI GCGATG 2 cut(s) 2400, 2967
BtsCI GGATG 9 cut(s) 943, 1183, 1567, 1579, 1720, 1914, 2526, 2788, 2814
BtsIMutI CAGTG 3 cut(s) 1688, 2425, 2550
BtuMI TCGCGA 1 cut(s) 454
BveI ACCTGC 2 cut(s) 2890, 2945
Cac8I GCNNGC 3 cut(s) 445, 730, 3279
CaiI CAGNNNCTG 3 cut(s) 328, 662, 735
CciI TCATGA 2 cut(s) 76, 1836
CfoI GCGC 7 cut(s) 11, 20, 792, 1862, 1982, 2409, 3147
Cfr10I RCCGGY 2 cut(s) 612, 1991
Cfr13I GGNCC 6 cut(s) 1142, 1206, 1376, 1754, 2641, 3059
CseI GACGC 1 cut(s) 305
Csp6I GTAC 9 cut(s) 799, 1789, 1959, 2036, 2576, 2591, 2665, 2965, 3207
CviQI GTAC 9 cut(s) 799, 1789, 1959, 2036, 2576, 2591, 2665, 2965, 3207
DinI GGCGCC 1 cut(s) 1981
DrdI GACNNNNNNGTC 1 cut(s) 926
DseDI GACNNNNNNGTC 1 cut(s) 926
EaeI YGGCCR 2 cut(s) 228, 1931
EagI CGGCCG 1 cut(s) 1931
Eam1104I CTCTTC 4 cut(s) 454, 687, 860, 1485
EarI CTCTTC 4 cut(s) 454, 687, 860, 1485
Ecl136II GAGCTC 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 1931
Eco130I CCWWGG 4 cut(s) 794, 2019, 2220, 3037
Eco24I GRGCYC 5 cut(s) 43, 54, 265, 439, 1044
Eco31I GGTCTC 1 cut(s) 2508
Eco32I GATATC 1 cut(s) 1679
Eco47I GGWCC 3 cut(s) 1206, 1754, 3059
Eco52I CGGCCG 1 cut(s) 1931
Eco53kI GAGCTC 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 2580
Eco81I CCTNAGG 1 cut(s) 2646
Eco88I CYCGRG 1 cut(s) 1353
EcoICRI GAGCTC 1 cut(s) 41
EcoNI CCTNNNNNAGG 1 cut(s) 2796
EcoO109I RGGNCCY 1 cut(s) 1142
EcoRV GATATC 1 cut(s) 1679
EcoT14I CCWWGG 4 cut(s) 794, 2019, 2220, 3037
EcoT38I GRGCYC 5 cut(s) 43, 54, 265, 439, 1044
EgeI GGCGCC 1 cut(s) 1981
EheI GGCGCC 1 cut(s) 1981
ErhI CCWWGG 4 cut(s) 794, 2019, 2220, 3037
Esp3I CGTCTC 1 cut(s) 323
FalI AAGNNNNNCTT 6 cut(s) 753, 785, 1065, 1097, 2428, 2460
FaqI GGGAC 4 cut(s) 707, 1740, 2424, 2596
FauNDI CATATG 2 cut(s) 2362, 3055
FbaI TGATCA 1 cut(s) 1839
FblI GTMKAC 1 cut(s) 176
FokI GGATG 9 cut(s) 950, 1190, 1574, 1586, 1727, 1921, 2513, 2775, 2821
FriOI GRGCYC 5 cut(s) 43, 54, 265, 439, 1044
FspBI CTAG 8 cut(s) 47, 495, 725, 1007, 1232, 1725, 1820, 2604
FspI TGCGCA 1 cut(s) 2408
GlaI GCGC 7 cut(s) 10, 19, 791, 1861, 1981, 2408, 3146
GsuI CTGGAG 3 cut(s) 305, 709, 2364
HaeII RGCGCY 4 cut(s) 12, 793, 1983, 3148
HaeIII GGCC 9 cut(s) 230, 493, 1143, 1173, 1332, 1377, 1933, 2642, 3081
HapII CCGG 7 cut(s) 490, 613, 1219, 1309, 1934, 1983, 1992
HgaI GACGC 1 cut(s) 305
HhaI GCGC 7 cut(s) 11, 20, 792, 1862, 1982, 2409, 3147
Hin1I GRCGYC 2 cut(s) 1965, 1980
Hin6I GCGC 7 cut(s) 9, 18, 790, 1860, 1980, 2407, 3145
HinP1I GCGC 7 cut(s) 9, 18, 790, 1860, 1980, 2407, 3145
HincII GTYRAC 2 cut(s) 1012, 1537
HindII GTYRAC 2 cut(s) 1012, 1537
HindIII AAGCTT 1 cut(s) 210
HpaII CCGG 7 cut(s) 490, 613, 1219, 1309, 1934, 1983, 1992
Hpy166II GTNNAC 4 cut(s) 177, 1012, 1537, 2036
Hpy8I GTNNAC 4 cut(s) 177, 1012, 1537, 2036
Hpy99I CGWCG 2 cut(s) 1967, 1970
HpyCH4III ACNGT 9 cut(s) 397, 664, 1390, 1534, 1922, 2474, 2635, 2815, 3274
HpyCH4IV ACGT 5 cut(s) 696, 1347, 1791, 1965, 2863
HpySE526I ACGT 5 cut(s) 696, 1347, 1791, 1965, 2863
Hsp92I GRCGYC 2 cut(s) 1965, 1980
HspAI GCGC 7 cut(s) 9, 18, 790, 1860, 1980, 2407, 3145
KasI GGCGCC 1 cut(s) 1979
Ksp22I TGATCA 1 cut(s) 1839
LguI GCTCTTC 2 cut(s) 454, 687
LmnI GCTCC 4 cut(s) 710, 1066, 2371, 2428
Lsp1109I GCAGC 8 cut(s) 664, 960, 1399, 1860, 2009, 2104, 2378, 2716
MaeI CTAG 8 cut(s) 47, 495, 725, 1007, 1232, 1725, 1820, 2604
MaeII ACGT 5 cut(s) 696, 1347, 1791, 1965, 2863
MaeIII GTNAC 4 cut(s) 568, 1213, 1348, 2284
MfeI CAATTG 3 cut(s) 241, 2151, 2376
MflI RGATCY 3 cut(s) 1271, 1289, 2705
MhlI GDGCHC 7 cut(s) 43, 54, 265, 439, 606, 1044, 2425
Mly113I GGCGCC 1 cut(s) 1980
MlyI GAGTC 3 cut(s) 1222, 1812, 2156
MmeI TCCRAC 1 cut(s) 607
MroXI GAANNNNTTC 1 cut(s) 3049
MslI CAYNNNNRTG 3 cut(s) 915, 1464, 2499
MspCI CTTAAG 1 cut(s) 3202
MspI CCGG 7 cut(s) 490, 613, 1219, 1309, 1934, 1983, 1992
MunI CAATTG 3 cut(s) 241, 2151, 2376
Mva1269I GAATGC 2 cut(s) 1496, 2263
MvnI CGCG 4 cut(s) 18, 454, 1862, 2844
NarI GGCGCC 1 cut(s) 1980
NciI CCSGG 2 cut(s) 490, 1219
NdeI CATATG 2 cut(s) 2362, 3055
NmuCI GTSAC 3 cut(s) 568, 1213, 2284
NruI TCGCGA 1 cut(s) 454
NsbI TGCGCA 1 cut(s) 2408
NspI RCATGY 4 cut(s) 2042, 2489, 2504, 3021
NspV TTCGAA 1 cut(s) 185
PagI TCATGA 2 cut(s) 76, 1836
PaqCI CACCTGC 2 cut(s) 2890, 2945
PciI ACATGT 2 cut(s) 2038, 2500
PciSI GCTCTTC 2 cut(s) 454, 687
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 2 cut(s) 1496, 2263
PdmI GAANNNNTTC 1 cut(s) 3049
PflFI GACNNNGTC 1 cut(s) 1967
PflMI CCANNNNNTGG 2 cut(s) 328, 735
PleI GAGTC 3 cut(s) 1222, 1811, 2155
PluTI GGCGCC 1 cut(s) 1983
PpsI GAGTC 3 cut(s) 1222, 1811, 2155
Ppu21I YACGTR 1 cut(s) 2864
PscI ACATGT 2 cut(s) 2038, 2500
PshBI ATTAAT 1 cut(s) 1185
PsiI TTATAA 1 cut(s) 3233
Psp124BI GAGCTC 1 cut(s) 43
PspPI GGNCC 6 cut(s) 1142, 1206, 1376, 1754, 2641, 3059
PstNI CAGNNNCTG 3 cut(s) 328, 662, 735
PsuI RGATCY 3 cut(s) 1271, 1289, 2705
PsyI GACNNNGTC 1 cut(s) 1967
RruI TCGCGA 1 cut(s) 454
RsaI GTAC 9 cut(s) 800, 1790, 1960, 2037, 2577, 2592, 2666, 2966, 3208
RsaNI GTAC 9 cut(s) 799, 1789, 1959, 2036, 2576, 2591, 2665, 2965, 3207
RseI CAYNNNNRTG 3 cut(s) 915, 1464, 2499
SacI GAGCTC 1 cut(s) 43
SapI GCTCTTC 2 cut(s) 454, 687
Sau96I GGNCC 6 cut(s) 1142, 1206, 1376, 1754, 2641, 3059
SchI GAGTC 3 cut(s) 1222, 1812, 2156
SduI GDGCHC 7 cut(s) 43, 54, 265, 439, 606, 1044, 2425
SfcI CTRYAG 1 cut(s) 177
SfoI GGCGCC 1 cut(s) 1981
SfuI TTCGAA 1 cut(s) 185
SinI GGWCC 3 cut(s) 1206, 1754, 3059
SmiMI CAYNNNNRTG 3 cut(s) 915, 1464, 2499
SmlI CTYRAG 4 cut(s) 438, 932, 2056, 3202
SmoI CTYRAG 4 cut(s) 438, 932, 2056, 3202
SsiI CCGC 6 cut(s) 158, 610, 1015, 1913, 2842, 3095
SspDI GGCGCC 1 cut(s) 1979
SspI AATATT 1 cut(s) 1507
SspMI CTAG 8 cut(s) 47, 495, 725, 1007, 1232, 1725, 1820, 2604
SstI GAGCTC 1 cut(s) 43
StyI CCWWGG 4 cut(s) 794, 2019, 2220, 3037
TaaI ACNGT 9 cut(s) 397, 664, 1390, 1534, 1922, 2474, 2635, 2815, 3274
TaiI ACGT 5 cut(s) 699, 1350, 1794, 1968, 2866
TaqI TCGA 5 cut(s) 37, 185, 759, 783, 2298
TatI WGTACW 4 cut(s) 2035, 2664, 2964, 3206
TauI GCSGC 2 cut(s) 160, 1915
TscAI CASTG 3 cut(s) 1695, 2425, 2557
TseFI GTSAC 3 cut(s) 568, 1213, 2284
TseI GCWGC 8 cut(s) 677, 948, 1412, 1848, 1997, 2092, 2391, 2729
Tsp45I GTSAC 3 cut(s) 568, 1213, 2284
TspDTI ATGAA 9 cut(s) 242, 519, 809, 1047, 1579, 1593, 2763, 3042, 3293
TspRI CASTG 3 cut(s) 1695, 2425, 2557
Tth111I GACNNNGTC 1 cut(s) 1967
Van91I CCANNNNNTGG 2 cut(s) 328, 735
Vha464I CTTAAG 1 cut(s) 3202
VpaK11BI GGWCC 3 cut(s) 1206, 1754, 3059
VspI ATTAAT 1 cut(s) 1185
XagI CCTNNNNNAGG 1 cut(s) 2796
XapI RAATTY 5 cut(s) 31, 308, 477, 1405, 1615
XceI RCATGY 4 cut(s) 2042, 2489, 2504, 3021
XcmI CCANNNNNNNNNTGG 1 cut(s) 1708
XmiI GTMKAC 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 3049
XspI CTAG 8 cut(s) 47, 495, 725, 1007, 1232, 1725, 1820, 2604
ZraI GACGTC 1 cut(s) 1966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.