Rroxscaffold_7G00214430

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000108
Physical Location & Seq
Reverse (-)
65079872 .. 65087611
7740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214430.1

Sequence Viewer

Length: 1494 bp
ATGGTTTCTTACGAGTATATTCTGGGTGGGTTGTTAGCTTCTTTGCTGGGCTCTGTTTTCTTCATGATCATAAGTACTACTCTTGGTGGAAACAAAAAGGTTAAGGCTTTAAGTGGAGGAGTCAAGAGTAATGCGTTTGGAGACGGTGCGTTTCGGCCGGAAATGGAGGAGGAGAAAAGTACCGACGTCGTCATTGTCGGTGCCGGAGTTGCCGGTGCAGCTCTTGCTTACACTCTTGCCAAGGAAGGACGCCGGGTACATGTCATCGAAAGAGACTTGACTGAGCCGGATAGAATTGTTGGTGAGCTGTTGCAGCCTGGGGGTTATCTCAAATTAATTGAGTTGGGACTTGAGGACTGTGCAAATGAGTCCATTGATGCTCAGAAGGTGTTTGGTTATGCTCTTTACAAAGATGGAAAGGATACTATATTGTCTTATCCCTTAGAAAAATACAGTTCAGATGTGGCTGGAAGAAGTTTTCACAATGGTCGTTTCATTCAGAGAATGCGTGAAAAAGCTGTAACTCTTCCAAATGTAAAATTGGAACAAGGATCAGTGACGACACTAATCGAGGAAAAGGGCATTGTTAAAGGGGTGATTTACAAGAACAAGGCCGGAGAAGAGATGAGAACATATGCTCCACTAACTATTGTATGCGATGGCTGCTTTTCAAATCTACGCAAATCTCTCAGTGCTCCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGATCTTGGAGAACTGTGATCTTCCACATGCAAATCATGGACATGTGATTTTGGGAGACCCTTCAGCCATCCTATTTTATCCTATCAGTTCAACGGAGTGGCTCCTCAGGTTCCCCCACAACTGTACAATGCTTTTATGGCCGCTGTCAACAAACATCAAATCCATGCAAAACAAAAGCATGGCTGCTAATCCTGTTCCCACTCCTGGTGCAATTTTATTGGGGGATTCATTCAACATGAGGCATCCTTTAACTGGAGGAGGAATGACTGTGGCTCTTTCAGACATAGTTCTTCTCCGTGATCTTCTTAGACCGTTATGTAATCTCAATGATGCACCTGCTTGGTGCAATTACCTTGAATCATTTTATACTCTGCGTAAGCCTGTATCATCCACCATAAATACATTGGCAGGTGCTTTGTACAAGGTGTTTTGTGCCTCACCTGATCCAGCGAGACAGGAAATGCGTGAAGCATGTTTTGGCTATTTGAGCCTTGGAGGCATCTGCTCATATGGACCAGTATCGCTTCTCTCTGGTCTTAACCCTCGTCCACTGCACTTGGTTCTCCATTTCTTTGCTGTTGCTATCTATGGTGTCGGCCGCTTAATGCTTCCATTCCCTTCACCTAAACGCATGTGGCTTGGGGCTAGATTGATCTTGAGTGCATCAGGCATCATATTCCCCATTATAAAGGGCGAAGGAGTTAGACAAATGTTTTTTCCTGCCACAGTACCAGCATATTACAGAACTCCTCCTGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

497

Amino Acids

54.2

Weight (kDa)

8.62

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 61 - 252 6.6e-14 FAD binding domain
DAO PF01266 62 - 92 5.8e-08 FAD dependent oxidoreductase
FAD_binding_2 PF00890 62 - 91 1e-05 FAD binding domain
NAD_binding_8 PF13450 65 - 91 1.9e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 213 - 276 2.3e-24 Squalene epoxidase
SE PF08491 294 - 456 5.1e-64 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1418
AarI CACCTGC 2 cut(s) 1075, 1130
AatII GACGTC 1 cut(s) 189
Acc36I ACCTGC 2 cut(s) 1075, 1130
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 2 cut(s) 872, 1330
AclWI GGATC 2 cut(s) 559, 1169
AcoI YGGCCR 3 cut(s) 155, 869, 1327
AcuI CTGAAG 1 cut(s) 777
AcyI GRCGYC 2 cut(s) 186, 250
AfaI GTAC 6 cut(s) 76, 181, 258, 856, 1151, 1461
AfiI CCNNNNNNNGG 2 cut(s) 935, 983
AflIII ACRYGT 2 cut(s) 259, 772
AgsI TTSAA 5 cut(s) 672, 707, 822, 964, 1088
AjnI CCWGG 2 cut(s) 316, 934
AjuI GAANNNNNNNTTGG 2 cut(s) 1191, 1223
AloI GAACNNNNNNTCC 2 cut(s) 622, 654
AluBI AGCT 4 cut(s) 38, 221, 307, 518
AluI AGCT 4 cut(s) 38, 221, 307, 518
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 4 cut(s) 135, 267, 780, 1177
AlwI GGATC 2 cut(s) 559, 1169
AoxI GGCC 4 cut(s) 155, 612, 869, 1327
ApeKI GCWGC 4 cut(s) 218, 313, 663, 914
AseI ATTAAT 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 1244
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 4 cut(s) 314, 607, 1161, 1344
AvaII GGWCC 1 cut(s) 1244
AxyI CCTNAGG 1 cut(s) 836
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 4 cut(s) 230, 325, 650, 901
BccI CCATC 3 cut(s) 407, 653, 806
BciT130I CCWGG 2 cut(s) 318, 936
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 4 cut(s) 135, 267, 780, 1177
BfaI CTAG 1 cut(s) 1377
BfuAI ACCTGC 2 cut(s) 1075, 1130
BfuI GTATCC 1 cut(s) 415
BglI GCCNNNNNGGC 1 cut(s) 1227
BisI GCNGC 6 cut(s) 219, 314, 664, 872, 915, 1330
BlsI GCNGC 6 cut(s) 220, 315, 665, 873, 916, 1331
BmcAI AGTACT 1 cut(s) 76
Bme1390I CCNGG 3 cut(s) 254, 318, 936
Bme18I GGWCC 1 cut(s) 1244
BmgT120I GGNCC 1 cut(s) 1244
BmiI GGNNCC 3 cut(s) 202, 833, 842
BmrFI CCNGG 3 cut(s) 254, 318, 936
BmsI GCATC 6 cut(s) 367, 982, 1051, 1239, 1403, 1410
BpmI CTGGAG 1 cut(s) 1005
BpuEI CTTGAG 2 cut(s) 371, 1408
BpuMI CCSGG 1 cut(s) 254
BsaHI GRCGYC 2 cut(s) 186, 250
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 240, 317, 1222
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 2 cut(s) 935, 983
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 988, 1247
Bse21I CCTNAGG 1 cut(s) 836
BseBI CCWGG 2 cut(s) 318, 936
BseDI CCNNGG 3 cut(s) 240, 317, 1222
BseGI GGATG 3 cut(s) 798, 973, 1118
BseLI CCNNNNNNNGG 2 cut(s) 935, 983
BseMII CTCAG 4 cut(s) 273, 395, 703, 850
BseNI ACTGG 2 cut(s) 988, 1247
BseRI GAGGAG 6 cut(s) 132, 182, 185, 824, 1002, 1470
BseX3I CGGCCG 2 cut(s) 155, 1327
BseXI GCAGC 4 cut(s) 230, 325, 650, 901
BseYI CCCAGC 1 cut(s) 46
BsgI GTGCAG 2 cut(s) 237, 1268
Bsh1285I CGRYCG 2 cut(s) 158, 1330
BshFI GGCC 4 cut(s) 157, 614, 871, 1329
BshNI GGYRCC 1 cut(s) 200
BsiEI CGRYCG 2 cut(s) 158, 1330
BsiHKAI GWGCWC 1 cut(s) 697
BsiSI CCGG 6 cut(s) 158, 204, 213, 253, 287, 615
BslFI GGGAC 2 cut(s) 360, 696
BslI CCNNNNNNNGG 2 cut(s) 935, 983
BsmAI GTCTC 4 cut(s) 135, 267, 780, 1177
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 2 cut(s) 360, 696
BsmI GAATGC 1 cut(s) 510
BsnI GGCC 4 cut(s) 157, 614, 871, 1329
Bso31I GGTCTC 1 cut(s) 780
Bsp1286I GDGCHC 2 cut(s) 53, 697
Bsp1407I TGTACA 2 cut(s) 854, 1149
Bsp143I GATC 7 cut(s) 66, 551, 732, 748, 1030, 1174, 1383
BspACI CCGC 2 cut(s) 872, 1330
BspANI GGCC 4 cut(s) 157, 614, 871, 1329
BspCNI CTCAG 4 cut(s) 274, 394, 702, 849
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 3 cut(s) 202, 833, 842
BspMI ACCTGC 2 cut(s) 1075, 1130
BspPI GGATC 2 cut(s) 559, 1169
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 780
BsrFI RCCGGY 1 cut(s) 212
BsrGI TGTACA 2 cut(s) 854, 1149
BsrI ACTGG 2 cut(s) 988, 1247
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 3 cut(s) 240, 317, 1222
BssMI GATC 7 cut(s) 66, 551, 732, 748, 1030, 1174, 1383
BssNI GRCGYC 2 cut(s) 186, 250
BssT1I CCWWGG 2 cut(s) 240, 1222
Bst2UI CCWGG 2 cut(s) 318, 936
Bst4CI ACNGT 8 cut(s) 146, 359, 455, 746, 854, 1000, 1044, 1459
Bst6I CTCTTC 2 cut(s) 531, 615
BstACI GRCGYC 2 cut(s) 186, 250
BstAPI GCANNNNNTGC 1 cut(s) 224
BstAUI TGTACA 2 cut(s) 854, 1149
BstDEI CTNAG 6 cut(s) 282, 381, 442, 689, 836, 1037
BstF5I GGATG 3 cut(s) 798, 973, 1118
BstKTI GATC 7 cut(s) 69, 554, 735, 751, 1033, 1177, 1386
BstMAI GTCTC 4 cut(s) 135, 267, 780, 1177
BstMBI GATC 7 cut(s) 66, 551, 732, 748, 1030, 1174, 1383
BstMCI CGRYCG 2 cut(s) 158, 1330
BstMWI GCNNNNNNNGC 8 cut(s) 209, 218, 224, 313, 663, 868, 1218, 1227
BstNI CCWGG 2 cut(s) 318, 936
BstNSI RCATGY 5 cut(s) 263, 761, 776, 1206, 1366
BstSCI CCNGG 3 cut(s) 252, 316, 934
BstV1I GCAGC 4 cut(s) 230, 325, 650, 901
BstZI CGGCCG 2 cut(s) 155, 1327
Bsu36I CCTNAGG 1 cut(s) 836
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 4 cut(s) 157, 614, 871, 1329
BtgZI GCGATG 1 cut(s) 672
BtsCI GGATG 3 cut(s) 798, 973, 1118
BtsI GCAGTG 1 cut(s) 1280
BtsIMutI CAGTG 3 cut(s) 561, 697, 1280
BveI ACCTGC 2 cut(s) 1075, 1130
CciI TCATGA 1 cut(s) 63
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 1244
CseI GACGC 1 cut(s) 258
Csp6I GTAC 6 cut(s) 75, 180, 257, 855, 1150, 1460
CspCI CAANNNNNGTGG 2 cut(s) 1269, 1304
CviQI GTAC 6 cut(s) 75, 180, 257, 855, 1150, 1460
DdeI CTNAG 6 cut(s) 282, 381, 442, 689, 836, 1037
DpnI GATC 7 cut(s) 68, 553, 734, 750, 1032, 1176, 1385
DpnII GATC 7 cut(s) 66, 551, 732, 748, 1030, 1174, 1383
EaeI YGGCCR 3 cut(s) 155, 869, 1327
EagI CGGCCG 2 cut(s) 155, 1327
Eam1104I CTCTTC 2 cut(s) 531, 615
EarI CTCTTC 2 cut(s) 531, 615
EclXI CGGCCG 2 cut(s) 155, 1327
Eco130I CCWWGG 2 cut(s) 240, 1222
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 780
Eco47I GGWCC 1 cut(s) 1244
Eco52I CGGCCG 2 cut(s) 155, 1327
Eco57I CTGAAG 1 cut(s) 777
Eco81I CCTNAGG 1 cut(s) 836
EcoRII CCWGG 2 cut(s) 316, 934
EcoT14I CCWWGG 2 cut(s) 240, 1222
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 2 cut(s) 240, 1222
Esp3I CGTCTC 1 cut(s) 135
FalI AAGNNNNNCTT 2 cut(s) 700, 732
FaqI GGGAC 2 cut(s) 360, 696
FauNDI CATATG 2 cut(s) 634, 1240
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 6 cut(s) 219, 314, 664, 872, 915, 1330
FokI GGATG 3 cut(s) 785, 960, 1105
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 6 cut(s) 219, 314, 664, 872, 915, 1330
FspBI CTAG 1 cut(s) 1377
GluI GCNGC 6 cut(s) 219, 314, 664, 872, 915, 1330
GsaI CCCAGC 1 cut(s) 50
GsuI CTGGAG 1 cut(s) 1005
HaeIII GGCC 4 cut(s) 157, 614, 871, 1329
HapII CCGG 6 cut(s) 158, 204, 213, 253, 287, 615
HgaI GACGC 1 cut(s) 258
Hin1I GRCGYC 2 cut(s) 186, 250
HincII GTYRAC 1 cut(s) 879
HindII GTYRAC 1 cut(s) 879
HinfI GANTC 4 cut(s) 120, 368, 956, 1088
HpaII CCGG 6 cut(s) 158, 204, 213, 253, 287, 615
HphI GGTGA 4 cut(s) 314, 607, 1161, 1344
Hpy166II GTNNAC 2 cut(s) 879, 1280
Hpy188I TCNGA 4 cut(s) 384, 460, 501, 1012
Hpy188III TCNNGA 3 cut(s) 64, 124, 1387
Hpy8I GTNNAC 2 cut(s) 879, 1280
Hpy99I CGWCG 2 cut(s) 188, 191
HpyAV CCTTC 5 cut(s) 239, 379, 801, 1359, 1421
HpyCH4III ACNGT 8 cut(s) 146, 359, 455, 746, 854, 1000, 1044, 1459
HpyCH4IV ACGT 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 8 cut(s) 209, 218, 224, 313, 663, 868, 1218, 1227
HpyF3I CTNAG 6 cut(s) 282, 381, 442, 689, 836, 1037
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 2 cut(s) 186, 250
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 7 cut(s) 66, 551, 732, 748, 1030, 1174, 1383
LmnI GCTCC 3 cut(s) 643, 700, 837
Lsp1109I GCAGC 4 cut(s) 230, 325, 650, 901
LweI GCATC 6 cut(s) 367, 982, 1051, 1239, 1403, 1410
MaeI CTAG 1 cut(s) 1377
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 520, 556
MalI GATC 7 cut(s) 68, 553, 734, 750, 1032, 1176, 1385
MboI GATC 7 cut(s) 66, 551, 732, 748, 1030, 1174, 1383
MboII GAAGA 7 cut(s) 52, 483, 518, 632, 743, 1013, 1025
MhlI GDGCHC 2 cut(s) 53, 697
MluCI AATT 6 cut(s) 294, 332, 336, 539, 942, 1078
MlyI GAGTC 2 cut(s) 129, 377
MseI TTAA 7 cut(s) 102, 110, 335, 588, 980, 1269, 1334
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 1 cut(s) 874
MspI CCGG 6 cut(s) 158, 204, 213, 253, 287, 615
MspR9I CCNGG 3 cut(s) 254, 318, 936
Mva1269I GAATGC 1 cut(s) 510
MvaI CCWGG 2 cut(s) 318, 936
MwoI GCNNNNNNNGC 8 cut(s) 209, 218, 224, 313, 663, 868, 1218, 1227
NciI CCSGG 1 cut(s) 254
NdeI CATATG 2 cut(s) 634, 1240
NdeII GATC 7 cut(s) 66, 551, 732, 748, 1030, 1174, 1383
NlaIV GGNNCC 3 cut(s) 202, 833, 842
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 5 cut(s) 263, 761, 776, 1206, 1366
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 2 cut(s) 1075, 1130
PciI ACATGT 2 cut(s) 259, 772
PctI GAATGC 1 cut(s) 510
PfeI GAWTC 2 cut(s) 956, 1088
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 6 cut(s) 220, 315, 665, 873, 916, 1331
PleI GAGTC 2 cut(s) 128, 376
PpsI GAGTC 2 cut(s) 128, 376
PscI ACATGT 2 cut(s) 259, 772
PshBI ATTAAT 1 cut(s) 335
PsiI TTATAA 1 cut(s) 1418
Psp6I CCWGG 2 cut(s) 316, 934
PspFI CCCAGC 1 cut(s) 46
PspGI CCWGG 2 cut(s) 316, 934
PspN4I GGNNCC 3 cut(s) 202, 833, 842
PspPI GGNCC 1 cut(s) 1244
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 6 cut(s) 76, 181, 258, 856, 1151, 1461
RsaNI GTAC 6 cut(s) 75, 180, 257, 855, 1150, 1460
RseI CAYNNNNRTG 1 cut(s) 771
SaqAI TTAA 7 cut(s) 102, 110, 335, 588, 980, 1269, 1334
SatI GCNGC 6 cut(s) 219, 314, 664, 872, 915, 1330
Sau3AI GATC 7 cut(s) 66, 551, 732, 748, 1030, 1174, 1383
Sau96I GGNCC 1 cut(s) 1244
ScaI AGTACT 1 cut(s) 76
SchI GAGTC 2 cut(s) 129, 377
ScrFI CCNGG 3 cut(s) 254, 318, 936
SduI GDGCHC 2 cut(s) 53, 697
SfaNI GCATC 6 cut(s) 367, 982, 1051, 1239, 1403, 1410
SinI GGWCC 1 cut(s) 1244
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 2 cut(s) 350, 1387
SmoI CTYRAG 2 cut(s) 350, 1387
Sse9I AATT 6 cut(s) 294, 332, 336, 539, 942, 1078
SsiI CCGC 2 cut(s) 872, 1330
SspMI CTAG 1 cut(s) 1377
StyD4I CCNGG 3 cut(s) 252, 316, 934
StyI CCWWGG 2 cut(s) 240, 1222
TaaI ACNGT 8 cut(s) 146, 359, 455, 746, 854, 1000, 1044, 1459
TaiI ACGT 1 cut(s) 189
TaqI TCGA 2 cut(s) 267, 570
TasI AATT 6 cut(s) 294, 332, 336, 539, 942, 1078
TatI WGTACW 3 cut(s) 74, 854, 1149
TauI GCSGC 2 cut(s) 874, 1332
TfiI GAWTC 2 cut(s) 956, 1088
Tru1I TTAA 7 cut(s) 102, 110, 335, 588, 980, 1269, 1334
Tru9I TTAA 7 cut(s) 102, 110, 335, 588, 980, 1269, 1334
TscAI CASTG 3 cut(s) 561, 697, 1287
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 4 cut(s) 218, 313, 663, 914
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 4 cut(s) 52, 484, 948, 1478
TspGWI ACGGA 2 cut(s) 839, 1016
TspRI CASTG 3 cut(s) 561, 697, 1287
Tth111I GACNNNGTC 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 1244
VspI ATTAAT 1 cut(s) 335
XceI RCATGY 5 cut(s) 263, 761, 776, 1206, 1366
XcmI CCANNNNNNNNNTGG 1 cut(s) 1132
XspI CTAG 1 cut(s) 1377
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.