Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000108

Physical Location & Seq

Genomic Coordinates Reverse (-)

72265220 .. 72269041

Gene Structure 3822 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00134720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGATGGCGACAATGGTGGCGAACGATCCGTATGTTGTGTGGACGTTCTTTGCTTCTTTATTGGGTTTTCTTCTTCTCTCCCTACGCCGCCGACATAATTACGGTAATGCCACCGCCAAGGACAAGGGAAAGGTTAAAAGCATTAACAAGACGACCGAGGATCATGTTTCTGAAGAATGTGTGAAGAGCTCCGGCGACGGAGACTTCCGGCAGGAGTACGGCTCCGGCACTGACGTCATCATCGTTGGCGCCGGTGTTGCTGGCGCTGCTCTTGCACACACCCTTGGCAAGGAGGGAAGACGTGTTCGTGTGATTGAAAGAGACTTGACAGAGCCGGATCGAATTGTTGGCGAACTTCTCCAACCTGGGGGATACCTGAAGCTGATTGACTTGGGACTTGAAGATTGTGTTGAGGATATCGATGCTCAGCGAGTTCTTGGATATGCTCTTTTTAAGGATGGGAAGAATACTACACTGTCCTATCCCCTGGAACATTTCCACTCAGATGTGGCGGGTAGAAGTTTCCACAATGGGCGATTCATACAGAGGATGAGAGAAAAAGCTGCTACTCTTCCCAAGCTTGTATCCTTATGTGTTCAATGTATATTTCCAGTGTACAACTGGAGCAAGGTACAGTCACATCCCTGCTTGAAGAAAATGGGACAATTACGGGGGGTTCAGTACAAAGCTAAGGATGGTCAAGAACTTCAAGCTCATGCTCCTCTTACAATTGTCTGCGATGGTTGCTTCTCAAATCTGCGCCGCACTCTTTGTAAGCCTCAGGTAGAAGTGCCCTCTTGTTTTGTGGGATTAATTTTGGAAAATTGTGAACTCCCATTTGCAAATCACGGGCATGTTATTCTTGCTGATCCATCTCCAATCCTGTTTTACCAAATCAGTAGTACAGAGGTCCGGTGTTTAGTTGATGTGCCCGGCCAGAAAGTACCTCCCATCACTAATGGGACAATGGCTAACTATTTGAAGAATGTGGTAGCTCCACAGCTTCCACCTGAACTGCATGATCCCTTCATATCTGCAATTGACAAAGGAAGTATTAGAACTATGCCGAATAGAAGCATGCCAGCCAATCCGCAGCCAACTCCTGGAGCCCTTTTAATGGGTGATGCTTTCAATATGCGTCATCCTTTAACTGGTGGAGGAATGACTGTGGCACTATCGGACATTGTTATACTCAGGGATCTTCTTAAACCATTACGTATAAATGATGCTTCTTCATTGTGCAACTACCTAGAATCCTTTTATACCTTGCGCAAGGTGCCCTATACAAGGTGTTCTCTGCCTCCCCCTGACCAAGCAAGGAAGGAGATGCGCCAAGCATGCTTTGACTATCTGAGCCTTGGAGGTGCCTGTTCAACTGGACCAGTGGCTCTGCTCTCTGGTCTAAATCCTCGCCCGCTGAGTTTAGTTCTCCATTTTTTTGCTGTTGCGATATATGGTGTTGGACGTTTGCTTCTACCATTTCCTTCACCGAAACGCGTGTGGATTGGAGTGAGATTAATCTGGAGCGCAGTAGGCATAATATTTCCCATCATCAAGGCAGAAGGTGTGAGACAGATGTTCTTTCCTGCTACTATTCCAGCTTGTCATAGGGCTCCTCCTGCTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004497 GO:0004506 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006629 GO:0006694 GO:0006950 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0012505 GO:0016125 GO:0016126 GO:0016491 GO:0016705 GO:0016709 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130

Pfam Domains

Protein Families

Protein Analysis

542

Amino Acids

59.59

Weight (kDa)

8.96

Isoelectric Point (pI)

45.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	77 - 399	5e-14	FAD binding domain
SE	PF08491	239 - 501	3.5e-104	Squalene epoxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000108)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22830 AT4G37760
fragaria_vesca	FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica	MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica	Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis	pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis	RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata	RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora	Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii	Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa	Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha	Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana	Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	237
Acc16I	TGCGCA	1 cut(s)	1271
AccB1I	GGYRCC	3 cut(s)	248, 1276, 1364
AccB7I	CCANNNNNTGG	1 cut(s)	1103
AccII	CGCG	1 cut(s)	1497
AciI	CCGC	6 cut(s)	88, 114, 512, 763, 1091, 1415
AclWI	GGATC	6 cut(s)	20, 168, 345, 863, 1016, 1206
AcoI	YGGCCR	1 cut(s)	934
AcuI	CTGAAG	2 cut(s)	192, 398
AcyI	GRCGYC	2 cut(s)	234, 249
AfaI	GTAC	6 cut(s)	218, 617, 633, 683, 904, 945
AfiI	CCNNNNNNNGG	6 cut(s)	289, 367, 1103, 1117, 1151, 1287
AflIII	ACRYGT	2 cut(s)	301, 1495
AgsI	TTSAA	8 cut(s)	317, 401, 599, 652, 710, 982, 1132, 1374
AjiI	CACGTC	1 cut(s)	302
AjnI	CCWGG	3 cut(s)	364, 486, 1102
AloI	GAACNNNNNNTCC	2 cut(s)	288, 320
AluBI	AGCT	9 cut(s)	189, 382, 563, 580, 689, 713, 995, 1003, 1601
AluI	AGCT	9 cut(s)	189, 382, 563, 580, 689, 713, 995, 1003, 1601
Alw21I	GWGCWC	1 cut(s)	191
Alw26I	GTCTC	3 cut(s)	195, 315, 1564
AlwI	GGATC	6 cut(s)	20, 168, 345, 863, 1016, 1206
AoxI	GGCC	1 cut(s)	934
ApeKI	GCWGC	3 cut(s)	266, 563, 1093
AseI	ATTAAT	2 cut(s)	812, 1517
AspLEI	GCGC	6 cut(s)	251, 266, 762, 1272, 1332, 1529
AspS9I	GGNCC	2 cut(s)	910, 1379
AsuC2I	CCSGG	1 cut(s)	933
AsuHPI	GGTGA	2 cut(s)	1133, 1479
AvaII	GGWCC	2 cut(s)	910, 1379
AxyI	CCTNAGG	1 cut(s)	780
BaeGI	GKGCMC	3 cut(s)	795, 933, 1281
BanI	GGYRCC	3 cut(s)	248, 1276, 1364
BanII	GRGCYC	3 cut(s)	191, 1111, 1615
BbsI	GAAGAC	1 cut(s)	304
Bbv12I	GWGCWC	1 cut(s)	191
BbvI	GCAGC	3 cut(s)	253, 550, 1105
BccI	CCATC	6 cut(s)	452, 689, 734, 880, 959, 1556
BceAI	ACGGC	1 cut(s)	235
BciT130I	CCWGG	3 cut(s)	366, 488, 1104
BciVI	GTATCC	2 cut(s)	365, 595
BcnI	CCSGG	1 cut(s)	933
BcoDI	GTCTC	3 cut(s)	195, 315, 1564
BfaI	CTAG	1 cut(s)	1250
BfoI	RGCGCY	2 cut(s)	252, 267
BfuI	GTATCC	2 cut(s)	365, 595
BisI	GCNGC	5 cut(s)	88, 267, 564, 763, 1094
BlpI	GCTNAGC	1 cut(s)	426
BlsI	GCNGC	5 cut(s)	89, 268, 565, 764, 1095
Bme1390I	CCNGG	4 cut(s)	366, 488, 933, 1104
Bme18I	GGWCC	2 cut(s)	910, 1379
BmgBI	CACGTC	1 cut(s)	302
BmgT120I	GGNCC	2 cut(s)	910, 1379
BmiI	GGNNCC	6 cut(s)	223, 250, 1108, 1278, 1366, 1614
BmrFI	CCNGG	4 cut(s)	366, 488, 933, 1104
BmsI	GCATC	4 cut(s)	412, 1114, 1216, 1317
BpiI	GAAGAC	1 cut(s)	304
BplI	GAGNNNNNCTC	2 cut(s)	206, 238
BpmI	CTGGAG	3 cut(s)	643, 1125, 1543
Bpu10I	CCTNAGC	1 cut(s)	690
Bpu1102I	GCTNAGC	1 cut(s)	426
BpuMI	CCSGG	1 cut(s)	933
Bsa29I	ATCGAT	1 cut(s)	420
BsaAI	YACGTR	1 cut(s)	1217
BsaHI	GRCGYC	2 cut(s)	234, 249
BsaJI	CCNNGG	6 cut(s)	117, 156, 283, 365, 486, 1357
BsaWI	WCCGGW	1 cut(s)	912
Bsc4I	CCNNNNNNNGG	6 cut(s)	289, 367, 1103, 1117, 1151, 1287
Bse118I	RCCGGY	1 cut(s)	251
Bse1I	ACTGG	5 cut(s)	611, 626, 1156, 1381, 1382
Bse21I	CCTNAGG	1 cut(s)	780
BseBI	CCWGG	3 cut(s)	366, 488, 1104
BseCI	ATCGAT	1 cut(s)	420
BseDI	CCNNGG	6 cut(s)	117, 156, 283, 365, 486, 1357
BseGI	GGATG	5 cut(s)	463, 555, 640, 700, 1141
BseLI	CCNNNNNNNGG	6 cut(s)	289, 367, 1103, 1117, 1151, 1287
BseMII	CTCAG	6 cut(s)	440, 516, 794, 1207, 1343, 1409
BseNI	ACTGG	5 cut(s)	611, 626, 1156, 1381, 1382
BseRI	GAGGAG	2 cut(s)	711, 1605
BseSI	GKGCMC	3 cut(s)	795, 933, 1281
BseXI	GCAGC	3 cut(s)	253, 550, 1105
Bsh1236I	CGCG	1 cut(s)	1497
Bsh1285I	CGRYCG	1 cut(s)	156
BshFI	GGCC	1 cut(s)	936
BshNI	GGYRCC	3 cut(s)	248, 1276, 1364
BshVI	ATCGAT	1 cut(s)	420
BsiEI	CGRYCG	1 cut(s)	156
BsiHKAI	GWGCWC	1 cut(s)	191
BsiSI	CCGG	7 cut(s)	192, 208, 225, 252, 335, 913, 933
BslFI	GGGAC	3 cut(s)	408, 675, 976
BslI	CCNNNNNNNGG	6 cut(s)	289, 367, 1103, 1117, 1151, 1287
BsmAI	GTCTC	3 cut(s)	195, 315, 1564
BsmFI	GGGAC	3 cut(s)	408, 675, 976
BsnI	GGCC	1 cut(s)	936
Bsp1286I	GDGCHC	6 cut(s)	191, 795, 933, 1111, 1281, 1615
Bsp1407I	TGTACA	1 cut(s)	615
Bsp143I	GATC	6 cut(s)	25, 160, 337, 868, 1021, 1198
Bsp1720I	GCTNAGC	1 cut(s)	426
BspACI	CCGC	6 cut(s)	88, 114, 512, 763, 1091, 1415
BspANI	GGCC	1 cut(s)	936
BspCNI	CTCAG	6 cut(s)	439, 515, 793, 1206, 1344, 1410
BspDI	ATCGAT	1 cut(s)	420
BspFNI	CGCG	1 cut(s)	1497
BspLI	GGNNCC	6 cut(s)	223, 250, 1108, 1278, 1366, 1614
BspPI	GGATC	6 cut(s)	20, 168, 345, 863, 1016, 1206
BspQI	GCTCTTC	1 cut(s)	179
BspT107I	GGYRCC	3 cut(s)	248, 1276, 1364
BsrFI	RCCGGY	1 cut(s)	251
BsrGI	TGTACA	1 cut(s)	615
BsrI	ACTGG	5 cut(s)	611, 626, 1156, 1381, 1382
BssAI	RCCGGY	1 cut(s)	251
BssECI	CCNNGG	6 cut(s)	117, 156, 283, 365, 486, 1357
BssMI	GATC	6 cut(s)	25, 160, 337, 868, 1021, 1198
BssNI	GRCGYC	2 cut(s)	234, 249
BssT1I	CCWWGG	3 cut(s)	117, 283, 1357
Bst2UI	CCWGG	3 cut(s)	366, 488, 1104
Bst4CI	ACNGT	4 cut(s)	104, 477, 636, 1168
Bst6I	CTCTTC	2 cut(s)	179, 576
BstACI	GRCGYC	2 cut(s)	234, 249
BstAUI	TGTACA	1 cut(s)	615
BstBAI	YACGTR	1 cut(s)	1217
BstC8I	GCNNGC	5 cut(s)	262, 1079, 1083, 1339, 1415
BstDEI	CTNAG	7 cut(s)	426, 502, 690, 780, 1193, 1352, 1418
BstENI	CCTNNNNNAGG	2 cut(s)	287, 1285
BstF5I	GGATG	5 cut(s)	463, 555, 640, 700, 1141
BstFNI	CGCG	1 cut(s)	1497
BstH2I	RGCGCY	2 cut(s)	252, 267
BstHHI	GCGC	6 cut(s)	251, 266, 762, 1272, 1332, 1529
BstKTI	GATC	6 cut(s)	28, 163, 340, 871, 1024, 1201
BstMAI	GTCTC	3 cut(s)	195, 315, 1564
BstMBI	GATC	6 cut(s)	25, 160, 337, 868, 1021, 1198
BstMCI	CGRYCG	1 cut(s)	156
BstMWI	GCNNNNNNNGC	8 cut(s)	257, 266, 272, 744, 1276, 1338, 1533, 1619
BstNI	CCWGG	3 cut(s)	366, 488, 1104
BstNSI	RCATGY	3 cut(s)	857, 1081, 1341
BstSCI	CCNGG	4 cut(s)	364, 486, 931, 1102
BstSLI	GKGCMC	3 cut(s)	795, 933, 1281
BstSNI	TACGTA	1 cut(s)	1217
BstUI	CGCG	1 cut(s)	1497
BstV1I	GCAGC	3 cut(s)	253, 550, 1105
BstV2I	GAAGAC	1 cut(s)	304
BstX2I	RGATCY	1 cut(s)	1198
BstYI	RGATCY	1 cut(s)	1198
Bsu15I	ATCGAT	1 cut(s)	420
Bsu36I	CCTNAGG	1 cut(s)	780
BsuI	GTATCC	2 cut(s)	365, 595
BsuRI	GGCC	1 cut(s)	936
BsuTUI	ATCGAT	1 cut(s)	420
BtgZI	GCGATG	1 cut(s)	753
BtrI	CACGTC	1 cut(s)	302
BtsCI	GGATG	5 cut(s)	463, 555, 640, 700, 1141
BtsIMutI	CAGTG	4 cut(s)	228, 473, 618, 1389
Cac8I	GCNNGC	5 cut(s)	262, 1079, 1083, 1339, 1415
CfoI	GCGC	6 cut(s)	251, 266, 762, 1272, 1332, 1529
Cfr10I	RCCGGY	1 cut(s)	251
Cfr13I	GGNCC	2 cut(s)	910, 1379
ClaI	ATCGAT	1 cut(s)	420
CseI	GACGC	1 cut(s)	1127
Csp6I	GTAC	6 cut(s)	217, 616, 632, 682, 903, 944
CviAII	CATG	6 cut(s)	164, 716, 854, 1019, 1078, 1338
CviQI	GTAC	6 cut(s)	217, 616, 632, 682, 903, 944
DdeI	CTNAG	7 cut(s)	426, 502, 690, 780, 1193, 1352, 1418
DinI	GGCGCC	1 cut(s)	250
DpnI	GATC	6 cut(s)	27, 162, 339, 870, 1023, 1200
DpnII	GATC	6 cut(s)	25, 160, 337, 868, 1021, 1198
EaeI	YGGCCR	1 cut(s)	934
Eam1104I	CTCTTC	2 cut(s)	179, 576
EarI	CTCTTC	2 cut(s)	179, 576
Ecl136II	GAGCTC	1 cut(s)	189
Eco105I	TACGTA	1 cut(s)	1217
Eco130I	CCWWGG	3 cut(s)	117, 283, 1357
Eco24I	GRGCYC	3 cut(s)	191, 1111, 1615
Eco32I	GATATC	1 cut(s)	418
Eco47I	GGWCC	2 cut(s)	910, 1379
Eco53kI	GAGCTC	1 cut(s)	189
Eco57I	CTGAAG	2 cut(s)	192, 398
Eco81I	CCTNAGG	1 cut(s)	780
EcoICRI	GAGCTC	1 cut(s)	189
EcoNI	CCTNNNNNAGG	2 cut(s)	287, 1285
EcoRII	CCWGG	3 cut(s)	364, 486, 1102
EcoRV	GATATC	1 cut(s)	418
EcoT14I	CCWWGG	3 cut(s)	117, 283, 1357
EcoT38I	GRGCYC	3 cut(s)	191, 1111, 1615
EgeI	GGCGCC	1 cut(s)	250
EheI	GGCGCC	1 cut(s)	250
ErhI	CCWWGG	3 cut(s)	117, 283, 1357
FaeI	CATG	6 cut(s)	167, 719, 857, 1022, 1081, 1341
FaqI	GGGAC	3 cut(s)	408, 675, 976
FatI	CATG	6 cut(s)	163, 715, 853, 1018, 1077, 1337
FauI	CCCGC	2 cut(s)	505, 1422
Fnu4HI	GCNGC	5 cut(s)	88, 267, 564, 763, 1094
FokI	GGATG	5 cut(s)	470, 562, 627, 707, 1128
FriOI	GRGCYC	3 cut(s)	191, 1111, 1615
Fsp4HI	GCNGC	5 cut(s)	88, 267, 564, 763, 1094
FspBI	CTAG	1 cut(s)	1250
FspI	TGCGCA	1 cut(s)	1271
GlaI	GCGC	6 cut(s)	250, 265, 761, 1271, 1331, 1528
GluI	GCNGC	5 cut(s)	88, 267, 564, 763, 1094
GsuI	CTGGAG	3 cut(s)	643, 1125, 1543
HaeII	RGCGCY	2 cut(s)	252, 267
HaeIII	GGCC	1 cut(s)	936
HapII	CCGG	7 cut(s)	192, 208, 225, 252, 335, 913, 933
HgaI	GACGC	1 cut(s)	1127
HhaI	GCGC	6 cut(s)	251, 266, 762, 1272, 1332, 1529
Hin1I	GRCGYC	2 cut(s)	234, 249
Hin1II	CATG	6 cut(s)	167, 719, 857, 1022, 1081, 1341
Hin6I	GCGC	6 cut(s)	249, 264, 760, 1270, 1330, 1527
HinP1I	GCGC	6 cut(s)	249, 264, 760, 1270, 1330, 1527
HindIII	AAGCTT	1 cut(s)	578
HinfI	GANTC	2 cut(s)	537, 1253
HpaII	CCGG	7 cut(s)	192, 208, 225, 252, 335, 913, 933
HphI	GGTGA	2 cut(s)	1133, 1479
Hpy166II	GTNNAC	3 cut(s)	42, 616, 830
Hpy188I	TCNGA	4 cut(s)	172, 505, 1180, 1353
Hpy188III	TCNNGA	2 cut(s)	701, 1522
Hpy8I	GTNNAC	3 cut(s)	42, 616, 830
Hpy99I	CGWCG	1 cut(s)	200
HpyAV	CCTTC	4 cut(s)	1036, 1315, 1494, 1556
HpyCH4III	ACNGT	4 cut(s)	104, 477, 636, 1168
HpyCH4IV	ACGT	5 cut(s)	44, 234, 301, 1216, 1465
HpyCH4V	TGCA	5 cut(s)	275, 842, 1018, 1037, 1242
HpyF10VI	GCNNNNNNNGC	8 cut(s)	257, 266, 272, 744, 1276, 1338, 1533, 1619
HpyF3I	CTNAG	7 cut(s)	426, 502, 690, 780, 1193, 1352, 1418
HpySE526I	ACGT	5 cut(s)	44, 234, 301, 1216, 1465
Hsp92I	GRCGYC	2 cut(s)	234, 249
Hsp92II	CATG	6 cut(s)	167, 719, 857, 1022, 1081, 1341
HspAI	GCGC	6 cut(s)	249, 264, 760, 1270, 1330, 1527
KasI	GGCGCC	1 cut(s)	248
Kzo9I	GATC	6 cut(s)	25, 160, 337, 868, 1021, 1198
LguI	GCTCTTC	1 cut(s)	179
LmnI	GCTCC	8 cut(s)	194, 227, 624, 724, 1000, 1106, 1524, 1618
Lsp1109I	GCAGC	3 cut(s)	253, 550, 1105
LweI	GCATC	4 cut(s)	412, 1114, 1216, 1317
MaeI	CTAG	1 cut(s)	1250
MaeII	ACGT	5 cut(s)	44, 234, 301, 1216, 1465
MaeIII	GTNAC	1 cut(s)	636
MalI	GATC	6 cut(s)	27, 162, 339, 870, 1023, 1200
MboI	GATC	6 cut(s)	25, 160, 337, 868, 1021, 1198
MfeI	CAATTG	2 cut(s)	729, 1038
MflI	RGATCY	1 cut(s)	1198
MhlI	GDGCHC	6 cut(s)	191, 795, 933, 1111, 1281, 1615
MluCI	AATT	7 cut(s)	97, 342, 665, 729, 813, 823, 1038
MluI	ACGCGT	1 cut(s)	1495
Mly113I	GGCGCC	1 cut(s)	249
MmeI	TCCRAC	2 cut(s)	385, 1441
MseI	TTAA	8 cut(s)	135, 144, 453, 812, 1115, 1148, 1206, 1517
MslI	CAYNNNNRTG	2 cut(s)	504, 852
MspA1I	CMGCKG	1 cut(s)	1417
MspI	CCGG	7 cut(s)	192, 208, 225, 252, 335, 913, 933
MspR9I	CCNGG	4 cut(s)	366, 488, 933, 1104
MunI	CAATTG	2 cut(s)	729, 1038
MvaI	CCWGG	3 cut(s)	366, 488, 1104
MvnI	CGCG	1 cut(s)	1497
MwoI	GCNNNNNNNGC	8 cut(s)	257, 266, 272, 744, 1276, 1338, 1533, 1619
NarI	GGCGCC	1 cut(s)	249
NciI	CCSGG	1 cut(s)	933
NdeII	GATC	6 cut(s)	25, 160, 337, 868, 1021, 1198
NlaIII	CATG	6 cut(s)	167, 719, 857, 1022, 1081, 1341
NlaIV	GGNNCC	6 cut(s)	223, 250, 1108, 1278, 1366, 1614
NmuCI	GTSAC	1 cut(s)	636
NsbI	TGCGCA	1 cut(s)	1271
NspI	RCATGY	3 cut(s)	857, 1081, 1341
PaeI	GCATGC	2 cut(s)	1081, 1341
PciSI	GCTCTTC	1 cut(s)	179
PcsI	WCGNNNNNNNCGW	1 cut(s)	240
PfeI	GAWTC	2 cut(s)	537, 1253
PflMI	CCANNNNNTGG	1 cut(s)	1103
PfoI	TCCNGGA	1 cut(s)	1102
PkrI	GCNGC	5 cut(s)	89, 268, 565, 764, 1095
PluTI	GGCGCC	1 cut(s)	252
Ppu21I	YACGTR	1 cut(s)	1217
PshBI	ATTAAT	2 cut(s)	812, 1517
Psp124BI	GAGCTC	1 cut(s)	191
Psp6I	CCWGG	3 cut(s)	364, 486, 1102
PspGI	CCWGG	3 cut(s)	364, 486, 1102
PspN4I	GGNNCC	6 cut(s)	223, 250, 1108, 1278, 1366, 1614
PspPI	GGNCC	2 cut(s)	910, 1379
PsuI	RGATCY	1 cut(s)	1198
RsaI	GTAC	6 cut(s)	218, 617, 633, 683, 904, 945
RsaNI	GTAC	6 cut(s)	217, 616, 632, 682, 903, 944
RseI	CAYNNNNRTG	2 cut(s)	504, 852
SacI	GAGCTC	1 cut(s)	191
SapI	GCTCTTC	1 cut(s)	179
SaqAI	TTAA	8 cut(s)	135, 144, 453, 812, 1115, 1148, 1206, 1517
SatI	GCNGC	5 cut(s)	88, 267, 564, 763, 1094
Sau3AI	GATC	6 cut(s)	25, 160, 337, 868, 1021, 1198
Sau96I	GGNCC	2 cut(s)	910, 1379
ScrFI	CCNGG	4 cut(s)	366, 488, 933, 1104
SduI	GDGCHC	6 cut(s)	191, 795, 933, 1111, 1281, 1615
SfaNI	GCATC	4 cut(s)	412, 1114, 1216, 1317
SfoI	GGCGCC	1 cut(s)	250
SgrAI	CRCCGGYG	1 cut(s)	251
SinI	GGWCC	2 cut(s)	910, 1379
SmiMI	CAYNNNNRTG	2 cut(s)	504, 852
SnaBI	TACGTA	1 cut(s)	1217
SphI	GCATGC	2 cut(s)	1081, 1341
Sse9I	AATT	7 cut(s)	97, 342, 665, 729, 813, 823, 1038
SsiI	CCGC	6 cut(s)	88, 114, 512, 763, 1091, 1415
SspDI	GGCGCC	1 cut(s)	248
SspI	AATATT	1 cut(s)	1542
SspMI	CTAG	1 cut(s)	1250
SstI	GAGCTC	1 cut(s)	191
StyD4I	CCNGG	4 cut(s)	364, 486, 931, 1102
StyI	CCWWGG	3 cut(s)	117, 283, 1357
TaaI	ACNGT	4 cut(s)	104, 477, 636, 1168
TaiI	ACGT	5 cut(s)	47, 237, 304, 1219, 1468
TaqI	TCGA	2 cut(s)	340, 420
TaqII	GACCGA	1 cut(s)	170
TasI	AATT	7 cut(s)	97, 342, 665, 729, 813, 823, 1038
TatI	WGTACW	3 cut(s)	615, 681, 902
TauI	GCSGC	2 cut(s)	90, 765
TfiI	GAWTC	2 cut(s)	537, 1253
Tru1I	TTAA	8 cut(s)	135, 144, 453, 812, 1115, 1148, 1206, 1517
Tru9I	TTAA	8 cut(s)	135, 144, 453, 812, 1115, 1148, 1206, 1517
TscAI	CASTG	4 cut(s)	235, 480, 618, 1389
TseFI	GTSAC	1 cut(s)	636
TseI	GCWGC	3 cut(s)	266, 563, 1093
Tsp45I	GTSAC	1 cut(s)	636
TspDTI	ATGAA	3 cut(s)	529, 1018, 1224
TspGWI	ACGGA	2 cut(s)	18, 213
TspRI	CASTG	4 cut(s)	235, 480, 618, 1389
Van91I	CCANNNNNTGG	1 cut(s)	1103
VpaK11BI	GGWCC	2 cut(s)	910, 1379
VspI	ATTAAT	2 cut(s)	812, 1517
XagI	CCTNNNNNAGG	2 cut(s)	287, 1285
XceI	RCATGY	3 cut(s)	857, 1081, 1341
XcmI	CCANNNNNNNNNTGG	1 cut(s)	618
XspI	CTAG	1 cut(s)	1250
ZraI	GACGTC	1 cut(s)	235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.