RchiOBHm_Chr6g0246421

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000108
Physical Location & Seq
Forward (+)
2120043 .. 2120934
892 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22087

Sequence Viewer

Length: 513 bp
ATGGTTTCGTACGAATATGTTTTGGCTGGGGTGTTGGTTTTTTTGCTGGGCTCTATTTTCTTCATGATCATCAGCTCTACTTTGGGGGGAAAGAAACAATTCAAGGTTTTAGAGTTAGTAAAGAGTGATGGGTTCGGAAACGCTAAGTCTCTGCCCGAAATGGAGGAGGAGAAAAGTACCGATGTCGTCATTGTCGGCGCCAGAGTTGCTGGTGCAGCTCTTGCATACACTCTTGCCAAGGAAGGACGACGTGTACATGTAATTGAAAGAGACTCGAGTGAGCCAGACAGAATTGTTGGGGAGCTGTTGCAGCCTGGGGGATATCTCAAATTGATTGAGTTGGGTCTTGAATATAGTGCAAATGAGTCGATTGATGCCCAGAAAGTGTTTGGTTATGCTCTTTACAAAGATGGGAAGGATATAACACTCTCTTATCCCTTGGAAAGTTACCGTTCAGATGTACGTGGTCGGGAGGAGTTTCCACAATGGGCGTTTCATTCAAAGAATGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

170

Amino Acids

18.84

Weight (kDa)

5.31

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 60 - 144 3.9e-06 FAD binding domain
DAO PF01266 61 - 91 9.6e-06 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 4 cut(s) 11, 178, 255, 462
AflIII ACRYGT 2 cut(s) 250, 256
AgsI TTSAA 4 cut(s) 103, 266, 350, 501
AjiI CACGTC 1 cut(s) 251
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 3 cut(s) 75, 218, 304
AluI AGCT 3 cut(s) 75, 218, 304
Alw26I GTCTC 2 cut(s) 153, 264
Ama87I CYCGRG 1 cut(s) 274
ApeKI GCWGC 2 cut(s) 215, 310
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 1 cut(s) 200
AvaI CYCGRG 1 cut(s) 274
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 227, 322
BccI CCATC 2 cut(s) 122, 404
BcgI CGANNNNNNTGC 2 cut(s) 348, 382
BciT130I CCWGG 1 cut(s) 315
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 153, 264
BfoI RGCGCY 1 cut(s) 201
BisI GCNGC 2 cut(s) 216, 311
BlsI GCNGC 2 cut(s) 217, 312
Bme1390I CCNGG 1 cut(s) 315
BmeT110I CYCGRG 1 cut(s) 274
BmgBI CACGTC 1 cut(s) 251
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 364
BsaAI YACGTR 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 237, 314, 438
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 237, 314, 438
BseRI GAGGAG 3 cut(s) 179, 182, 488
BseXI GCAGC 2 cut(s) 227, 322
BseYI CCCAGC 2 cut(s) 26, 46
BsgI GTGCAG 1 cut(s) 234
BshNI GGYRCC 1 cut(s) 197
BsiHKCI CYCGRG 1 cut(s) 274
BsiWI CGTACG 1 cut(s) 9
BsmAI GTCTC 2 cut(s) 153, 264
BsmI GAATGC 1 cut(s) 511
BsoBI CYCGRG 1 cut(s) 274
Bsp1286I GDGCHC 1 cut(s) 53
Bsp1407I TGTACA 1 cut(s) 253
Bsp143I GATC 1 cut(s) 66
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 197
BsrGI TGTACA 1 cut(s) 253
BssECI CCNNGG 3 cut(s) 237, 314, 438
BssMI GATC 1 cut(s) 66
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 237, 438
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 452
BstACI GRCGYC 1 cut(s) 198
BstAPI GCANNNNNTGC 1 cut(s) 221
BstAUI TGTACA 1 cut(s) 253
BstBAI YACGTR 1 cut(s) 464
BstDEI CTNAG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 2 cut(s) 153, 264
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 4 cut(s) 206, 215, 221, 310
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 313
BstV1I GCAGC 2 cut(s) 227, 322
BtrI CACGTC 1 cut(s) 251
CciI TCATGA 1 cut(s) 63
CfoI GCGC 1 cut(s) 200
Csp6I GTAC 4 cut(s) 10, 177, 254, 461
CviAII CATG 2 cut(s) 64, 257
CviJI RGCY 7 cut(s) 26, 51, 75, 218, 283, 304, 313
CviKI_1 RGCY 7 cut(s) 26, 51, 75, 218, 283, 304, 313
CviQI GTAC 4 cut(s) 10, 177, 254, 461
DdeI CTNAG 1 cut(s) 144
DinI GGCGCC 1 cut(s) 199
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
Eco130I CCWWGG 2 cut(s) 237, 438
Eco24I GRGCYC 1 cut(s) 53
Eco32I GATATC 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 313
EcoRV GATATC 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 237, 438
EcoT38I GRGCYC 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 2 cut(s) 237, 438
FaeI CATG 2 cut(s) 67, 260
FaiI YATR 7 cut(s) 18, 65, 226, 258, 354, 396, 422
FatI CATG 2 cut(s) 63, 256
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 216, 311
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 216, 311
GlaI GCGC 1 cut(s) 199
GluI GCNGC 2 cut(s) 216, 311
GsaI CCCAGC 2 cut(s) 30, 50
HaeII RGCGCY 1 cut(s) 201
HhaI GCGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 67, 260
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 2 cut(s) 272, 365
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 2 cut(s) 137, 457
Hpy188III TCNNGA 3 cut(s) 64, 347, 470
Hpy8I GTNNAC 1 cut(s) 254
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 2 cut(s) 236, 409
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4IV ACGT 2 cut(s) 250, 463
HpyCH4V TGCA 4 cut(s) 215, 224, 310, 359
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 215, 221, 310
HpyF3I CTNAG 1 cut(s) 144
HpySE526I ACGT 2 cut(s) 250, 463
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 67, 260
HspAI GCGC 1 cut(s) 198
KasI GGCGCC 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 301
LpnPI CCDG 8 cut(s) 12, 32, 195, 214, 297, 300, 327, 392
Lsp1109I GCAGC 2 cut(s) 227, 322
LweI GCATC 1 cut(s) 364
MaeII ACGT 2 cut(s) 250, 463
MaeIII GTNAC 1 cut(s) 446
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MboII GAAGA 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 4 cut(s) 98, 261, 291, 329
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 2 cut(s) 266, 374
MnlI CCTC 3 cut(s) 157, 160, 466
MroXI GAANNNNTTC 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 4 cut(s) 206, 215, 221, 310
NarI GGCGCC 1 cut(s) 198
NdeII GATC 1 cut(s) 66
NlaIII CATG 2 cut(s) 67, 260
NlaIV GGNNCC 1 cut(s) 199
NspI RCATGY 1 cut(s) 260
PaeR7I CTCGAG 1 cut(s) 274
PagI TCATGA 1 cut(s) 63
PciI ACATGT 1 cut(s) 256
PctI GAATGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 98
Pfl23II CGTACG 1 cut(s) 9
PkrI GCNGC 2 cut(s) 217, 312
PleI GAGTC 2 cut(s) 266, 373
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 2 cut(s) 266, 373
Ppu21I YACGTR 1 cut(s) 464
PscI ACATGT 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 313
PspFI CCCAGC 2 cut(s) 26, 46
PspGI CCWGG 1 cut(s) 313
PspLI CGTACG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 199
PspXI VCTCGAGB 1 cut(s) 274
RsaI GTAC 4 cut(s) 11, 178, 255, 462
RsaNI GTAC 4 cut(s) 10, 177, 254, 461
SatI GCNGC 2 cut(s) 216, 311
Sau3AI GATC 1 cut(s) 66
SchI GAGTC 2 cut(s) 266, 374
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 1 cut(s) 53
SetI ASST 6 cut(s) 77, 108, 220, 253, 306, 466
SfaNI GCATC 1 cut(s) 364
SfoI GGCGCC 1 cut(s) 199
Sfr274I CTCGAG 1 cut(s) 274
SlaI CTCGAG 1 cut(s) 274
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 4 cut(s) 98, 261, 291, 329
SspDI GGCGCC 1 cut(s) 197
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 2 cut(s) 237, 438
TaaI ACNGT 1 cut(s) 452
TaiI ACGT 2 cut(s) 253, 466
TaqI TCGA 2 cut(s) 275, 368
TasI AATT 4 cut(s) 98, 261, 291, 329
TatI WGTACW 1 cut(s) 253
TseI GCWGC 2 cut(s) 215, 310
TspDTI ATGAA 2 cut(s) 52, 485
XceI RCATGY 1 cut(s) 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 386
XhoI CTCGAG 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.