squalene
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 516 |
| AciI | CCGC | 3 cut(s) | 224, 259, 1008 |
| AcoI | YGGCCR | 1 cut(s) | 1005 |
| AcyI | GRCGYC | 1 cut(s) | 256 |
| AfaI | GTAC | 3 cut(s) | 589, 855, 870 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 152, 1028 |
| AgsI | TTSAA | 8 cut(s) | 103, 111, 137, 359, 578, 713, 876, 907 |
| AjnI | CCWGG | 2 cut(s) | 322, 1027 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 628, 660 |
| AluBI | AGCT | 6 cut(s) | 166, 313, 524, 631, 881, 940 |
| AluI | AGCT | 6 cut(s) | 166, 313, 524, 631, 881, 940 |
| Alw21I | GWGCWC | 1 cut(s) | 703 |
| Alw26I | GTCTC | 2 cut(s) | 273, 786 |
| AoxI | GGCC | 1 cut(s) | 1005 |
| ApeKI | GCWGC | 2 cut(s) | 524, 959 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 98, 395 |
| AspLEI | GCGC | 2 cut(s) | 209, 516 |
| AsuC2I | CCSGG | 1 cut(s) | 858 |
| AsuHPI | GGTGA | 5 cut(s) | 167, 320, 613, 792, 899 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 852, 885 |
| BanII | GRGCYC | 1 cut(s) | 53 |
| BbsI | GAAGAC | 1 cut(s) | 366 |
| Bbv12I | GWGCWC | 1 cut(s) | 703 |
| BbvCI | CCTCAGC | 1 cut(s) | 936 |
| BbvI | GCAGC | 2 cut(s) | 511, 946 |
| BccI | CCATC | 3 cut(s) | 413, 659, 812 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 506, 540 |
| BciT130I | CCWGG | 2 cut(s) | 324, 1029 |
| BciVI | GTATCC | 1 cut(s) | 421 |
| BclI | TGATCA | 1 cut(s) | 66 |
| BcnI | CCSGG | 1 cut(s) | 858 |
| BcoDI | GTCTC | 2 cut(s) | 273, 786 |
| BfaI | CTAG | 2 cut(s) | 35, 882 |
| BfoI | RGCGCY | 1 cut(s) | 210 |
| BfuI | GTATCC | 1 cut(s) | 421 |
| BisI | GCNGC | 5 cut(s) | 225, 259, 525, 960, 1008 |
| BlsI | GCNGC | 5 cut(s) | 226, 260, 526, 961, 1009 |
| Bme1390I | CCNGG | 3 cut(s) | 324, 858, 1029 |
| BmiI | GGNNCC | 1 cut(s) | 921 |
| BmrFI | CCNGG | 3 cut(s) | 324, 858, 1029 |
| BmsI | GCATC | 3 cut(s) | 132, 373, 659 |
| BpiI | GAAGAC | 1 cut(s) | 366 |
| BpmI | CTGGAG | 1 cut(s) | 642 |
| Bpu10I | CCTNAGC | 1 cut(s) | 936 |
| BpuEI | CTTGAG | 1 cut(s) | 304 |
| BpuMI | CCSGG | 1 cut(s) | 858 |
| BsaHI | GRCGYC | 1 cut(s) | 256 |
| BsaI | GGTCTC | 1 cut(s) | 786 |
| BsaJI | CCNNGG | 3 cut(s) | 323, 447, 912 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 628, 658 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 152, 1028 |
| BseBI | CCWGG | 2 cut(s) | 324, 1029 |
| BseDI | CCNNGG | 3 cut(s) | 323, 447, 912 |
| BseGI | GGATG | 5 cut(s) | 69, 147, 501, 674, 804 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 152, 1028 |
| BseMII | CTCAG | 4 cut(s) | 401, 845, 938, 950 |
| BseRI | GAGGAG | 2 cut(s) | 641, 912 |
| BseXI | GCAGC | 2 cut(s) | 511, 946 |
| BseYI | CCCAGC | 1 cut(s) | 46 |
| Bsh1236I | CGCG | 1 cut(s) | 516 |
| BshFI | GGCC | 1 cut(s) | 1007 |
| BsiHKAI | GWGCWC | 1 cut(s) | 703 |
| BsiSI | CCGG | 2 cut(s) | 210, 858 |
| BslFI | GGGAC | 1 cut(s) | 571 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 152, 1028 |
| BsmAI | GTCTC | 2 cut(s) | 273, 786 |
| BsmFI | GGGAC | 1 cut(s) | 571 |
| BsmI | GAATGC | 2 cut(s) | 261, 516 |
| BsnI | GGCC | 1 cut(s) | 1007 |
| Bso31I | GGTCTC | 1 cut(s) | 786 |
| Bsp1286I | GDGCHC | 2 cut(s) | 53, 703 |
| Bsp143I | GATC | 1 cut(s) | 66 |
| BspACI | CCGC | 3 cut(s) | 224, 259, 1008 |
| BspANI | GGCC | 1 cut(s) | 1007 |
| BspCNI | CTCAG | 4 cut(s) | 400, 844, 937, 949 |
| BspFNI | CGCG | 1 cut(s) | 516 |
| BspHI | TCATGA | 1 cut(s) | 63 |
| BspLI | GGNNCC | 1 cut(s) | 921 |
| BspTNI | GGTCTC | 1 cut(s) | 786 |
| BssECI | CCNNGG | 3 cut(s) | 323, 447, 912 |
| BssMI | GATC | 1 cut(s) | 66 |
| BssNI | GRCGYC | 1 cut(s) | 256 |
| BssT1I | CCWWGG | 2 cut(s) | 447, 912 |
| Bst2UI | CCWGG | 2 cut(s) | 324, 1029 |
| Bst4CI | ACNGT | 6 cut(s) | 155, 365, 438, 461, 562, 752 |
| Bst6I | CTCTTC | 3 cut(s) | 116, 171, 1023 |
| BstACI | GRCGYC | 1 cut(s) | 256 |
| BstC8I | GCNNGC | 1 cut(s) | 235 |
| BstDEI | CTNAG | 6 cut(s) | 387, 632, 695, 831, 924, 936 |
| BstF5I | GGATG | 5 cut(s) | 69, 147, 501, 674, 804 |
| BstFNI | CGCG | 1 cut(s) | 516 |
| BstH2I | RGCGCY | 1 cut(s) | 210 |
| BstHHI | GCGC | 2 cut(s) | 209, 516 |
| BstKTI | GATC | 1 cut(s) | 69 |
| BstMAI | GTCTC | 2 cut(s) | 273, 786 |
| BstMBI | GATC | 1 cut(s) | 66 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 215, 224, 230, 319, 669, 956 |
| BstNI | CCWGG | 2 cut(s) | 324, 1029 |
| BstSCI | CCNGG | 3 cut(s) | 322, 856, 1027 |
| BstUI | CGCG | 1 cut(s) | 516 |
| BstV1I | GCAGC | 2 cut(s) | 511, 946 |
| BstV2I | GAAGAC | 1 cut(s) | 366 |
| BsuI | GTATCC | 1 cut(s) | 421 |
| BsuRI | GGCC | 1 cut(s) | 1007 |
| BtgZI | GCGATG | 1 cut(s) | 678 |
| BtsCI | GGATG | 5 cut(s) | 69, 147, 501, 674, 804 |
| BtsIMutI | CAGTG | 1 cut(s) | 567 |
| Cac8I | GCNNGC | 1 cut(s) | 235 |
| CciI | TCATGA | 1 cut(s) | 63 |
| CfoI | GCGC | 2 cut(s) | 209, 516 |
| CseI | GACGC | 1 cut(s) | 264 |
| Csp6I | GTAC | 3 cut(s) | 588, 854, 869 |
| CviAII | CATG | 5 cut(s) | 64, 266, 773, 779, 1003 |
| CviQI | GTAC | 3 cut(s) | 588, 854, 869 |
| DdeI | CTNAG | 6 cut(s) | 387, 632, 695, 831, 924, 936 |
| DpnI | GATC | 1 cut(s) | 68 |
| DpnII | GATC | 1 cut(s) | 66 |
| EaeI | YGGCCR | 1 cut(s) | 1005 |
| Eam1104I | CTCTTC | 3 cut(s) | 116, 171, 1023 |
| EarI | CTCTTC | 3 cut(s) | 116, 171, 1023 |
| Eco130I | CCWWGG | 2 cut(s) | 447, 912 |
| Eco24I | GRGCYC | 1 cut(s) | 53 |
| Eco31I | GGTCTC | 1 cut(s) | 786 |
| EcoRII | CCWGG | 2 cut(s) | 322, 1027 |
| EcoT14I | CCWWGG | 2 cut(s) | 447, 912 |
| EcoT38I | GRGCYC | 1 cut(s) | 53 |
| ErhI | CCWWGG | 2 cut(s) | 447, 912 |
| FaeI | CATG | 5 cut(s) | 67, 269, 776, 782, 1006 |
| FaqI | GGGAC | 1 cut(s) | 571 |
| FatI | CATG | 5 cut(s) | 63, 265, 772, 778, 1002 |
| FauNDI | CATATG | 1 cut(s) | 640 |
| FbaI | TGATCA | 1 cut(s) | 66 |
| Fnu4HI | GCNGC | 5 cut(s) | 225, 259, 525, 960, 1008 |
| FokI | GGATG | 5 cut(s) | 56, 154, 488, 681, 791 |
| FriOI | GRGCYC | 1 cut(s) | 53 |
| Fsp4HI | GCNGC | 5 cut(s) | 225, 259, 525, 960, 1008 |
| FspBI | CTAG | 2 cut(s) | 35, 882 |
| GlaI | GCGC | 2 cut(s) | 208, 515 |
| GluI | GCNGC | 5 cut(s) | 225, 259, 525, 960, 1008 |
| GsaI | CCCAGC | 1 cut(s) | 50 |
| GsuI | CTGGAG | 1 cut(s) | 642 |
| HaeII | RGCGCY | 1 cut(s) | 210 |
| HaeIII | GGCC | 1 cut(s) | 1007 |
| HapII | CCGG | 2 cut(s) | 210, 858 |
| HgaI | GACGC | 1 cut(s) | 264 |
| HhaI | GCGC | 2 cut(s) | 209, 516 |
| Hin1I | GRCGYC | 1 cut(s) | 256 |
| Hin1II | CATG | 5 cut(s) | 67, 269, 776, 782, 1006 |
| Hin6I | GCGC | 2 cut(s) | 207, 514 |
| HinP1I | GCGC | 2 cut(s) | 207, 514 |
| HincII | GTYRAC | 1 cut(s) | 967 |
| HindII | GTYRAC | 1 cut(s) | 967 |
| HinfI | GANTC | 2 cut(s) | 374, 928 |
| HpaII | CCGG | 2 cut(s) | 210, 858 |
| HphI | GGTGA | 5 cut(s) | 167, 320, 613, 792, 899 |
| Hpy166II | GTNNAC | 1 cut(s) | 967 |
| Hpy188I | TCNGA | 5 cut(s) | 84, 390, 466, 927, 983 |
| Hpy188III | TCNNGA | 2 cut(s) | 64, 356 |
| Hpy8I | GTNNAC | 1 cut(s) | 967 |
| Hpy99I | CGWCG | 1 cut(s) | 194 |
| HpyAV | CCTTC | 5 cut(s) | 245, 590, 767, 807, 882 |
| HpyCH4III | ACNGT | 6 cut(s) | 155, 365, 438, 461, 562, 752 |
| HpyCH4IV | ACGT | 1 cut(s) | 192 |
| HpyCH4V | TGCA | 5 cut(s) | 237, 368, 527, 991, 1034 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 215, 224, 230, 319, 669, 956 |
| HpyF3I | CTNAG | 6 cut(s) | 387, 632, 695, 831, 924, 936 |
| HpySE526I | ACGT | 1 cut(s) | 192 |
| Hsp92I | GRCGYC | 1 cut(s) | 256 |
| Hsp92II | CATG | 5 cut(s) | 67, 269, 776, 782, 1006 |
| HspAI | GCGC | 2 cut(s) | 207, 514 |
| Ksp22I | TGATCA | 1 cut(s) | 66 |
| Kzo9I | GATC | 1 cut(s) | 66 |
| LmnI | GCTCC | 4 cut(s) | 628, 649, 706, 925 |
| Lsp1109I | GCAGC | 2 cut(s) | 511, 946 |
| LweI | GCATC | 3 cut(s) | 132, 373, 659 |
| MaeI | CTAG | 2 cut(s) | 35, 882 |
| MaeII | ACGT | 1 cut(s) | 192 |
| MaeIII | GTNAC | 3 cut(s) | 315, 562, 752 |
| MalI | GATC | 1 cut(s) | 68 |
| MboI | GATC | 1 cut(s) | 66 |
| MboII | GAAGA | 6 cut(s) | 52, 133, 188, 371, 590, 1010 |
| MhlI | GDGCHC | 2 cut(s) | 53, 703 |
| MluCI | AATT | 6 cut(s) | 98, 300, 338, 545, 573, 1035 |
| MlyI | GAGTC | 1 cut(s) | 383 |
| MnlI | CCTC | 5 cut(s) | 619, 730, 933, 945, 1026 |
| MroXI | GAANNNNTTC | 2 cut(s) | 98, 395 |
| MseI | TTAA | 2 cut(s) | 897, 1039 |
| MslI | CAYNNNNRTG | 1 cut(s) | 777 |
| MspA1I | CMGCKG | 1 cut(s) | 166 |
| MspI | CCGG | 2 cut(s) | 210, 858 |
| MspR9I | CCNGG | 3 cut(s) | 324, 858, 1029 |
| Mva1269I | GAATGC | 2 cut(s) | 261, 516 |
| MvaI | CCWGG | 2 cut(s) | 324, 1029 |
| MvnI | CGCG | 1 cut(s) | 516 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 215, 224, 230, 319, 669, 956 |
| NciI | CCSGG | 1 cut(s) | 858 |
| NdeI | CATATG | 1 cut(s) | 640 |
| NdeII | GATC | 1 cut(s) | 66 |
| NlaIII | CATG | 5 cut(s) | 67, 269, 776, 782, 1006 |
| NlaIV | GGNNCC | 1 cut(s) | 921 |
| NmuCI | GTSAC | 2 cut(s) | 562, 752 |
| PagI | TCATGA | 1 cut(s) | 63 |
| PctI | GAATGC | 2 cut(s) | 261, 516 |
| PdmI | GAANNNNTTC | 2 cut(s) | 98, 395 |
| PfeI | GAWTC | 1 cut(s) | 928 |
| PflFI | GACNNNGTC | 1 cut(s) | 194 |
| PkrI | GCNGC | 5 cut(s) | 226, 260, 526, 961, 1009 |
| PleI | GAGTC | 1 cut(s) | 382 |
| PpsI | GAGTC | 1 cut(s) | 382 |
| Psp6I | CCWGG | 2 cut(s) | 322, 1027 |
| PspFI | CCCAGC | 1 cut(s) | 46 |
| PspGI | CCWGG | 2 cut(s) | 322, 1027 |
| PspN4I | GGNNCC | 1 cut(s) | 921 |
| PsyI | GACNNNGTC | 1 cut(s) | 194 |
| PvuII | CAGCTG | 1 cut(s) | 166 |
| RsaI | GTAC | 3 cut(s) | 589, 855, 870 |
| RsaNI | GTAC | 3 cut(s) | 588, 854, 869 |
| RseI | CAYNNNNRTG | 1 cut(s) | 777 |
| SaqAI | TTAA | 2 cut(s) | 897, 1039 |
| SatI | GCNGC | 5 cut(s) | 225, 259, 525, 960, 1008 |
| Sau3AI | GATC | 1 cut(s) | 66 |
| SchI | GAGTC | 1 cut(s) | 383 |
| ScrFI | CCNGG | 3 cut(s) | 324, 858, 1029 |
| SduI | GDGCHC | 2 cut(s) | 53, 703 |
| SfaNI | GCATC | 3 cut(s) | 132, 373, 659 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 777 |
| SmlI | CTYRAG | 1 cut(s) | 283 |
| SmoI | CTYRAG | 1 cut(s) | 283 |
| Sse9I | AATT | 6 cut(s) | 98, 300, 338, 545, 573, 1035 |
| SsiI | CCGC | 3 cut(s) | 224, 259, 1008 |
| SspI | AATATT | 1 cut(s) | 902 |
| SspMI | CTAG | 2 cut(s) | 35, 882 |
| StyD4I | CCNGG | 3 cut(s) | 322, 856, 1027 |
| StyI | CCWWGG | 2 cut(s) | 447, 912 |
| TaaI | ACNGT | 6 cut(s) | 155, 365, 438, 461, 562, 752 |
| TaiI | ACGT | 1 cut(s) | 195 |
| TaqI | TCGA | 2 cut(s) | 273, 594 |
| TasI | AATT | 6 cut(s) | 98, 300, 338, 545, 573, 1035 |
| TauI | GCSGC | 3 cut(s) | 227, 261, 1010 |
| TfiI | GAWTC | 1 cut(s) | 928 |
| Tru1I | TTAA | 2 cut(s) | 897, 1039 |
| Tru9I | TTAA | 2 cut(s) | 897, 1039 |
| TscAI | CASTG | 1 cut(s) | 567 |
| TseFI | GTSAC | 2 cut(s) | 562, 752 |
| TseI | GCWGC | 2 cut(s) | 524, 959 |
| Tsp45I | GTSAC | 2 cut(s) | 562, 752 |
| TspDTI | ATGAA | 4 cut(s) | 52, 254, 264, 490 |
| TspRI | CASTG | 1 cut(s) | 567 |
| Tth111I | GACNNNGTC | 1 cut(s) | 194 |
| XmnI | GAANNNNTTC | 2 cut(s) | 98, 395 |
| XspI | CTAG | 2 cut(s) | 35, 882 |