Rh4AG168400

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000108
Physical Location & Seq
Forward (+)
42371123 .. 42373772
2650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG168400.1

Sequence Viewer

Length: 1494 bp
ATGGTGTCCTATGAGTATATTCTGGGTGGGTTCTTAGCTTCTTTGCTTGCCTTTGTTTTCTTCATGATCATCAGTTCTACTCTTGGTGGAAACAAAAAGGTCAAGGCTTTAAGTGGAGGAGCAAAGAGAAATGGGTTTCGGCCTGAAATGGAGGAGGACAAAAGTACCGACGTCGTCATTGTTGGCGCCGGAGTTGCCGGTGCAGCTCTAGCTTACACTCTTGCCAAGACATTGGTTTTTAATCAAAACTATCTCTTTGTGGCTGCAGACTGTGCAAATGAGTCCATTGATGCTCAGAAGGTGTTTGGTTATGCTCTTTACAAAGATGGGAAGGATACAAAACTGTCTTACCCCTTGGAGAAGTACAGTGCAGATGTCGCCGGGAGAAGTTTCCACAATGGTCGTTTCATTCAAAGAATGCGAGAAAAAGCTGCAACTCTTGCAAATGTAAAATTGGAACAAGGATCAGTGACAACACTAATTGAGGAGAAGGGCATTGTCAAAGGCGTGATTTACAAGAACAAGGCTGGAGAGGAGCTGAGAGCATATGCTCCACTAACAATCGTGTGTGATGGATGCTTTTCAAATCTGCGCAAATCTCTCAGTGCTCCAAAGGTTGAAAATCCCTCTTGTTTTGTTGGTTTGATTCTGGAGAACTGTGACCTTCCACATGCAAATCATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTGTTTTATCCCATCAGTAACACCGAGGTTCGTTGTTTGGTAGATGTACCTGGAACAAAAGTGCCTTCAGTAGCTAATGGTGAAATGGGCAAGTATTTGAAATCTGTGGTGGCTCCTCAGATTCCCCCACAGCTGCACAATGCTTTTATGGCAGCAGTAGAGGAAGGAAACATCAGAATAATGCAAAACAAAAGCATGGCTGCTAATCCTGTTCCCACTCCTGGTGCAATTTTATTGGGGGATGCATTCAACATGAGGCATCCATTAACAGGAGGAGGAATGACTGTGGCTCTTTCAGACATTGTTCTTCTCCGTAATCTTCTTAGACCCCTACGTGATCTCAGTGACGCAACTGCTTTGTGCAATTACCTTGAAGCATTCTACACACTTCGTAAGCCTGTGTCATCTACCATAAACACATTGGCAGGTGCTTTATACAAGGTGTTTTGTGCATCTCCTGATCCAGCAAAACAGGAAATGCGTGAAGCATGTTTTGACTATCTTAGCCTTGGAGGTATCTGTTCATATGGACCAGTATCTCTGCTCTCTGGTCTTAACCCTCGTCCCCTGCACTTATTTCTCCATTTCTTTGCCGTTGCCATCTATGGCGTCGGACGCTTAATGCTGCCCTTCCCTTCACCTCAGCGCATGTGGCTTGGGGCTAGATTGATCTTGAGTGCATCAAGCATCATATTCCCGATTATTAAGGGGGAAGGAGTTAGACAGATGTTCTTTCCTGCAACAGTGCCAGCATTTTACAGATCTCCTCCTCTTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

497

Amino Acids

53.91

Weight (kDa)

8.99

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 140 - 350 3e-09 FAD binding domain
SE PF08491 184 - 456 7.1e-119 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1130
AatII GACGTC 1 cut(s) 174
Acc16I TGCGCA 1 cut(s) 593
Acc36I ACCTGC 1 cut(s) 1130
AccB1I GGYRCC 1 cut(s) 185
AclWI GGATC 2 cut(s) 472, 1169
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 3 cut(s) 171, 186, 1323
AfaI GTAC 3 cut(s) 166, 365, 762
AfiI CCNNNNNNNGG 2 cut(s) 935, 983
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 6 cut(s) 413, 585, 620, 814, 964, 1088
AjnI CCWGG 2 cut(s) 763, 934
AluBI AGCT 7 cut(s) 38, 206, 212, 431, 538, 788, 847
AluI AGCT 7 cut(s) 38, 206, 212, 431, 538, 788, 847
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 1 cut(s) 693
AlwI GGATC 2 cut(s) 472, 1169
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 7 cut(s) 203, 263, 431, 847, 866, 914, 1339
AspLEI GCGC 3 cut(s) 188, 594, 1362
AspS9I GGNCC 1 cut(s) 1244
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 3 cut(s) 699, 806, 1344
AvaII GGWCC 1 cut(s) 1244
BaeI ACNNNNGTAYC 2 cut(s) 148, 181
BanI GGYRCC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 610
BbvCI CCTCAGC 1 cut(s) 1356
BbvI GCAGC 7 cut(s) 215, 250, 418, 834, 878, 901, 1326
BccI CCATC 5 cut(s) 320, 566, 719, 734, 1322
BceAI ACGGC 1 cut(s) 1292
BciT130I CCWGG 2 cut(s) 765, 936
BciVI GTATCC 1 cut(s) 328
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 693
BfaI CTAG 2 cut(s) 209, 1377
BfmI CTRYAG 1 cut(s) 264
BfoI RGCGCY 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 1130
BfuI GTATCC 1 cut(s) 328
BglII AGATCT 1 cut(s) 1475
BisI GCNGC 7 cut(s) 204, 264, 432, 848, 867, 915, 1340
BlsI GCNGC 7 cut(s) 205, 265, 433, 849, 868, 916, 1341
Bme1390I CCNGG 3 cut(s) 382, 765, 936
Bme18I GGWCC 1 cut(s) 1244
BmgT120I GGNCC 1 cut(s) 1244
BmiI GGNNCC 2 cut(s) 187, 828
BmrFI CCNGG 3 cut(s) 382, 765, 936
BmsI GCATC 7 cut(s) 280, 566, 946, 982, 1175, 1403, 1410
BpmI CTGGAG 2 cut(s) 549, 671
Bpu10I CCTNAGC 1 cut(s) 1356
BpuEI CTTGAG 1 cut(s) 1408
BpuMI CCSGG 1 cut(s) 382
BsaAI YACGTR 1 cut(s) 1049
BsaHI GRCGYC 3 cut(s) 171, 186, 1323
BsaI GGTCTC 1 cut(s) 693
BsaJI CCNNGG 3 cut(s) 354, 738, 1222
Bsc4I CCNNNNNNNGG 2 cut(s) 935, 983
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 1247
BseBI CCWGG 2 cut(s) 765, 936
BseDI CCNNGG 3 cut(s) 354, 738, 1222
BseGI GGATG 4 cut(s) 581, 711, 961, 973
BseLI CCNNNNNNNGG 2 cut(s) 935, 983
BseMII CTCAG 6 cut(s) 308, 530, 616, 845, 1069, 1370
BseNI ACTGG 1 cut(s) 1247
BseRI GAGGAG 8 cut(s) 132, 167, 500, 548, 819, 1002, 1470, 1473
BseXI GCAGC 7 cut(s) 215, 250, 418, 834, 878, 901, 1326
BsgI GTGCAG 4 cut(s) 222, 390, 833, 1268
BshFI GGCC 1 cut(s) 142
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 610
BsiSI CCGG 3 cut(s) 189, 198, 381
BslFI GGGAC 1 cut(s) 1263
BslI CCNNNNNNNGG 2 cut(s) 935, 983
BsmAI GTCTC 1 cut(s) 693
BsmFI GGGAC 1 cut(s) 1263
BsmI GAATGC 3 cut(s) 423, 959, 1091
BsnI GGCC 1 cut(s) 142
Bso31I GGTCTC 1 cut(s) 693
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 6 cut(s) 66, 464, 1051, 1174, 1383, 1475
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 6 cut(s) 307, 531, 615, 844, 1068, 1369
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 187, 828
BspMAI CTGCAG 1 cut(s) 268
BspMI ACCTGC 1 cut(s) 1130
BspPI GGATC 2 cut(s) 472, 1169
BspT107I GGYRCC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 693
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 1 cut(s) 1247
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 3 cut(s) 354, 738, 1222
BssMI GATC 6 cut(s) 66, 464, 1051, 1174, 1383, 1475
BssNI GRCGYC 3 cut(s) 171, 186, 1323
BssT1I CCWWGG 2 cut(s) 354, 1222
Bst2UI CCWGG 2 cut(s) 765, 936
Bst4CI ACNGT 6 cut(s) 272, 345, 368, 659, 1000, 1459
Bst6I CTCTTC 1 cut(s) 1491
BstACI GRCGYC 3 cut(s) 171, 186, 1323
BstAPI GCANNNNNTGC 2 cut(s) 272, 440
BstBAI YACGTR 1 cut(s) 1049
BstC8I GCNNGC 2 cut(s) 48, 1464
BstDEI CTNAG 9 cut(s) 34, 294, 539, 602, 831, 1037, 1055, 1217, 1356
BstF5I GGATG 4 cut(s) 581, 711, 961, 973
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 3 cut(s) 188, 594, 1362
BstKTI GATC 6 cut(s) 69, 467, 1054, 1177, 1386, 1478
BstMAI GTCTC 1 cut(s) 693
BstMBI GATC 6 cut(s) 66, 464, 1051, 1174, 1383, 1475
BstMWI GCNNNNNNNGC 9 cut(s) 194, 203, 209, 272, 377, 440, 863, 1329, 1366
BstNI CCWGG 2 cut(s) 765, 936
BstNSI RCATGY 4 cut(s) 674, 689, 1206, 1366
BstSCI CCNGG 3 cut(s) 380, 763, 934
BstSFI CTRYAG 1 cut(s) 264
BstV1I GCAGC 7 cut(s) 215, 250, 418, 834, 878, 901, 1326
BstX2I RGATCY 1 cut(s) 1475
BstXI CCANNNNNNTGG 1 cut(s) 232
BstYI RGATCY 1 cut(s) 1475
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 1 cut(s) 142
BtsCI GGATG 4 cut(s) 581, 711, 961, 973
BtsIMutI CAGTG 6 cut(s) 373, 474, 610, 1063, 1464, 1489
BveI ACCTGC 1 cut(s) 1130
Cac8I GCNNGC 2 cut(s) 48, 1464
CciI TCATGA 1 cut(s) 63
CfoI GCGC 3 cut(s) 188, 594, 1362
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 1244
CseI GACGC 3 cut(s) 1070, 1312, 1338
Csp6I GTAC 3 cut(s) 165, 364, 761
CviAII CATG 8 cut(s) 64, 671, 680, 686, 910, 967, 1203, 1363
CviQI GTAC 3 cut(s) 165, 364, 761
DdeI CTNAG 9 cut(s) 34, 294, 539, 602, 831, 1037, 1055, 1217, 1356
DinI GGCGCC 1 cut(s) 187
DpnI GATC 6 cut(s) 68, 466, 1053, 1176, 1385, 1477
DpnII GATC 6 cut(s) 66, 464, 1051, 1174, 1383, 1475
Eam1104I CTCTTC 1 cut(s) 1491
EarI CTCTTC 1 cut(s) 1491
Eco130I CCWWGG 2 cut(s) 354, 1222
Eco31I GGTCTC 1 cut(s) 693
Eco47I GGWCC 1 cut(s) 1244
Eco57I CTGAAG 1 cut(s) 765
EcoRII CCWGG 2 cut(s) 763, 934
EcoT14I CCWWGG 2 cut(s) 354, 1222
EcoT22I ATGCAT 1 cut(s) 961
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
ErhI CCWWGG 2 cut(s) 354, 1222
FaeI CATG 8 cut(s) 67, 674, 683, 689, 913, 970, 1206, 1366
FaqI GGGAC 1 cut(s) 1263
FatI CATG 8 cut(s) 63, 670, 679, 685, 909, 966, 1202, 1362
FauNDI CATATG 2 cut(s) 547, 1240
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 7 cut(s) 204, 264, 432, 848, 867, 915, 1340
FokI GGATG 4 cut(s) 588, 698, 960, 968
Fsp4HI GCNGC 7 cut(s) 204, 264, 432, 848, 867, 915, 1340
FspBI CTAG 2 cut(s) 209, 1377
FspI TGCGCA 1 cut(s) 593
GlaI GCGC 3 cut(s) 187, 593, 1361
GluI GCNGC 7 cut(s) 204, 264, 432, 848, 867, 915, 1340
GsuI CTGGAG 2 cut(s) 549, 671
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 1 cut(s) 142
HapII CCGG 3 cut(s) 189, 198, 381
HgaI GACGC 3 cut(s) 1070, 1312, 1338
HhaI GCGC 3 cut(s) 188, 594, 1362
Hin1I GRCGYC 3 cut(s) 171, 186, 1323
Hin1II CATG 8 cut(s) 67, 674, 683, 689, 913, 970, 1206, 1366
Hin6I GCGC 3 cut(s) 186, 592, 1360
HinP1I GCGC 3 cut(s) 186, 592, 1360
HinfI GANTC 3 cut(s) 281, 646, 835
HpaII CCGG 3 cut(s) 189, 198, 381
HphI GGTGA 3 cut(s) 699, 806, 1344
Hpy188I TCNGA 5 cut(s) 297, 834, 890, 1012, 1328
Hpy188III TCNNGA 5 cut(s) 64, 650, 1172, 1387, 1411
Hpy99I CGWCG 3 cut(s) 173, 176, 1328
HpyCH4III ACNGT 6 cut(s) 272, 345, 368, 659, 1000, 1459
HpyCH4IV ACGT 2 cut(s) 171, 1048
HpyF10VI GCNNNNNNNGC 9 cut(s) 194, 203, 209, 272, 377, 440, 863, 1329, 1366
HpyF3I CTNAG 9 cut(s) 34, 294, 539, 602, 831, 1037, 1055, 1217, 1356
HpySE526I ACGT 2 cut(s) 171, 1048
Hsp92I GRCGYC 3 cut(s) 171, 186, 1323
Hsp92II CATG 8 cut(s) 67, 674, 683, 689, 913, 970, 1206, 1366
HspAI GCGC 3 cut(s) 186, 592, 1360
KasI GGCGCC 1 cut(s) 185
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 6 cut(s) 66, 464, 1051, 1174, 1383, 1475
LmnI GCTCC 5 cut(s) 119, 535, 556, 613, 832
Lsp1109I GCAGC 7 cut(s) 215, 250, 418, 834, 878, 901, 1326
LweI GCATC 7 cut(s) 280, 566, 946, 982, 1175, 1403, 1410
MaeI CTAG 2 cut(s) 209, 1377
MaeII ACGT 2 cut(s) 171, 1048
MaeIII GTNAC 4 cut(s) 469, 659, 731, 1058
MalI GATC 6 cut(s) 68, 466, 1053, 1176, 1385, 1477
MboI GATC 6 cut(s) 66, 464, 1051, 1174, 1383, 1475
MboII GAAGA 4 cut(s) 52, 1013, 1025, 1478
MflI RGATCY 1 cut(s) 1475
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 4 cut(s) 452, 480, 942, 1078
Mly113I GGCGCC 1 cut(s) 186
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 1306
Mph1103I ATGCAT 1 cut(s) 961
MseI TTAA 6 cut(s) 110, 240, 980, 1269, 1334, 1419
MslI CAYNNNNRTG 1 cut(s) 684
MspA1I CMGCKG 1 cut(s) 847
MspI CCGG 3 cut(s) 189, 198, 381
MspR9I CCNGG 3 cut(s) 382, 765, 936
Mva1269I GAATGC 3 cut(s) 423, 959, 1091
MvaI CCWGG 2 cut(s) 765, 936
MwoI GCNNNNNNNGC 9 cut(s) 194, 203, 209, 272, 377, 440, 863, 1329, 1366
NarI GGCGCC 1 cut(s) 186
NciI CCSGG 1 cut(s) 382
NdeI CATATG 2 cut(s) 547, 1240
NdeII GATC 6 cut(s) 66, 464, 1051, 1174, 1383, 1475
NlaIII CATG 8 cut(s) 67, 674, 683, 689, 913, 970, 1206, 1366
NlaIV GGNNCC 2 cut(s) 187, 828
NmuCI GTSAC 3 cut(s) 469, 659, 1058
NsbI TGCGCA 1 cut(s) 593
NsiI ATGCAT 1 cut(s) 961
NspI RCATGY 4 cut(s) 674, 689, 1206, 1366
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 1 cut(s) 1130
PciI ACATGT 1 cut(s) 685
PctI GAATGC 3 cut(s) 423, 959, 1091
PfeI GAWTC 2 cut(s) 646, 835
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 7 cut(s) 205, 265, 433, 849, 868, 916, 1341
PleI GAGTC 1 cut(s) 289
PluTI GGCGCC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 289
Ppu21I YACGTR 1 cut(s) 1049
PscI ACATGT 1 cut(s) 685
Psp6I CCWGG 2 cut(s) 763, 934
PspGI CCWGG 2 cut(s) 763, 934
PspN4I GGNNCC 2 cut(s) 187, 828
PspPI GGNCC 1 cut(s) 1244
PstI CTGCAG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 1475
PsyI GACNNNGTC 1 cut(s) 173
PvuII CAGCTG 1 cut(s) 847
RsaI GTAC 3 cut(s) 166, 365, 762
RsaNI GTAC 3 cut(s) 165, 364, 761
RseI CAYNNNNRTG 1 cut(s) 684
SaqAI TTAA 6 cut(s) 110, 240, 980, 1269, 1334, 1419
SatI GCNGC 7 cut(s) 204, 264, 432, 848, 867, 915, 1340
Sau3AI GATC 6 cut(s) 66, 464, 1051, 1174, 1383, 1475
Sau96I GGNCC 1 cut(s) 1244
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 3 cut(s) 382, 765, 936
SduI GDGCHC 1 cut(s) 610
SfaNI GCATC 7 cut(s) 280, 566, 946, 982, 1175, 1403, 1410
SfcI CTRYAG 1 cut(s) 264
SfoI GGCGCC 1 cut(s) 187
SinI GGWCC 1 cut(s) 1244
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 1 cut(s) 1387
SmoI CTYRAG 1 cut(s) 1387
Sse9I AATT 4 cut(s) 452, 480, 942, 1078
SspDI GGCGCC 1 cut(s) 185
SspMI CTAG 2 cut(s) 209, 1377
StyD4I CCNGG 3 cut(s) 380, 763, 934
StyI CCWWGG 2 cut(s) 354, 1222
TaaI ACNGT 6 cut(s) 272, 345, 368, 659, 1000, 1459
TaiI ACGT 2 cut(s) 174, 1051
TasI AATT 4 cut(s) 452, 480, 942, 1078
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 2 cut(s) 646, 835
Tru1I TTAA 6 cut(s) 110, 240, 980, 1269, 1334, 1419
Tru9I TTAA 6 cut(s) 110, 240, 980, 1269, 1334, 1419
TscAI CASTG 5 cut(s) 373, 474, 610, 1063, 1464
TseFI GTSAC 3 cut(s) 469, 659, 1058
TseI GCWGC 7 cut(s) 203, 263, 431, 847, 866, 914, 1339
Tsp45I GTSAC 3 cut(s) 469, 659, 1058
TspDTI ATGAA 3 cut(s) 52, 397, 1227
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 5 cut(s) 373, 474, 610, 1063, 1464
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 1244
XceI RCATGY 4 cut(s) 674, 689, 1206, 1366
XcmI CCANNNNNNNNNTGG 1 cut(s) 1132
XspI CTAG 2 cut(s) 209, 1377
ZraI GACGTC 1 cut(s) 172
Zsp2I ATGCAT 1 cut(s) 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.