Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000108

Physical Location & Seq

Genomic Coordinates Forward (+)

17543325 .. 17547584

Gene Structure 4260 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1190300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1905 bp

ATGCTCCTACAAAACCCCCCACGTCCACGTCCACGCCCATGCCCATACCCACACCCACATCCCTCCTCCTCCATCCGCATCCACACCAATCAATTTCAAATTCACATCCCTCATCATCTACCTCCAATCTCTCTCTCCTCCCATAATTCCAAAACCAGAACCAGAACCAGAAGAAAGCCCAACATTACTAGTTGCACCCACTCCCGCATTTCTTCATCTGGGTCGCCGCCTTCTCCATCTGGGCCTTCTTCCTTCTCATCTGGGTCTTCTTCTTTCTCATCTGGGTTTTCTTCTAAGATGGCGGCAACTGTGGCGGTTGATCCGTACGTTCTCTGGACCTTCTTCGCCTCCCTCTTAGGTTTTCTGGCTCTCTCTGTTCTCCGTCGCCGGAATTCCGACCACGCCAAGAGCAATGCTCAGAAGAACAAGAGTAATACGACGATCGTTCAGGATAAAAGCATCAACAGCTCTGACGACGGAGAATTCCCCCCAATTCACGGCTCCGGCACCGATGTAATCGTCGTCGGCGCCGGCGTTGCTGGCGCTGCCCTCGCCCTTACCCTCGCCAAGGAGGGAAGACATGTTCATGTGATTGAAAGAGACTTGACGGAGCCAGATCGAATCGTCGGCGAGCTTCTCCAGCCAGGAGGGTACCTCAAATTGATGGAGTTGGGTCTTGAAGATTGTGTGGAGGAAATCGATGCTCAGCGAGTGGTCGGTTATGTTCTTTTTAAGGATGGGAAGAACACTCGGCTGACCTATCCTCTGGAACAGTTCCACTCCGATGTGGCGGGGAGAAGTTTCCACAATGGCCGATTCATACAGAGGATGAGGCAAAAAGCTGCAAAGCTTCCCAATGTACAACTGGAGCAAGGTACTGTAACGTCCCTGCTTGAAGTAAATGGGACAATTAAGGGGGTTCGATATAAAACCAAGGATGGTCAAGAACTTCAAGCGCACGCTCCTCTTACGATTGTTTGTGATGGTTGCTTCTCAAATCTGCGCCGCACTCTTTGCAAGCCTCAGGTAGAAGTGCCCTCTTGTTTCGTAGGATTAATCTTGGAAAATTGCAAACTCCCATTTGCAAATCATGGGCATGTTATTCTAGCGGATCCCTCTCCGATCCTGTTTTATCAAATCAGCAGTACAGAGGTCCGCTGTTTGGTTGATGTGCCCGGCCAAAGGGTACCTCCCATTGCCAATGGTGCAATGGCTAACTACTTGAAGACTGCGGTGGCTCCACAGGTTCCACCGGAACTTCATGATGCCTTTGTTGCTGCAATTGATAAGGGAAATATTAGAACAATGCCAAACAGAAGCATGCCAGCTAATCCTCAGCCAACTCCAGGAGCCCTTTTGCTGGGTGACGCTTTCAATATGCGCCATCCTTTAACCGGCGGAGGAATGACTGTGGCACTTTCTGATATTGTTGTACTCAAGGATCTTCTTAAGCCGCTGCGGAACCTACATGATGCAGCTTCGTTGTGCACATACCTAGAATCCTTTTATACCTTGCGCAAGCCGGTTGCATCTACGATAAATACCTTGGCAGGTGCCCTATACAAAGTGTTCTCCACCTCCCCTGATGAAGCAAGGACGGAGATGCGCCAAGCTTGTTTTGACTACCTAAGCCTTGGAGGTGCCTGTTCAACCGGACCAGTGGCTTTGCTCTCTGGTCTAAATCCTTGTCCGTTGAATTTAGTTCTTCATTTTTTTGCTGTGGCGGTATACGGTGTTGGTCGTTTGCTACTACCATTTCCTTCACCGAGACGCGTGTGGATTGGAGCTAGATTACTTTTGAGTGCATCGGGTATAATATTTCCCATCATCAAGGCTGAAGGAGTGAGACAGATGTTCTTTCCAGCTACCATTCCAGCTCATCATAGAGCTCCTCCTGCCGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004497 GO:0004506 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006629 GO:0006694 GO:0006950 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0012505 GO:0016125 GO:0016126 GO:0016491 GO:0016705 GO:0016709 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130

Pfam Domains

Protein Families

Protein Analysis

635

Amino Acids

68.8

Weight (kDa)

9.4

Isoelectric Point (pI)

48.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	170 - 498	5.5e-18	FAD binding domain
DAO	PF01266	171 - 201	1.3e-06	FAD dependent oxidoreductase
GIDA	PF01134	171 - 333	4e-07	Glucose inhibited division protein A
SE	PF08491	320 - 593	7.4e-120	Squalene epoxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000108)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22830 AT4G37760
fragaria_vesca	FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica	MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica	Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis	pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis	RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata	RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora	Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii	Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa	Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha	Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana	Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1541
Acc16I	TGCGCA	1 cut(s)	1517
Acc36I	ACCTGC	1 cut(s)	1541
Acc65I	GGTACC	2 cut(s)	651, 1186
AccB1I	GGYRCC	6 cut(s)	506, 527, 651, 1186, 1553, 1640
AccI	GTMKAC	1 cut(s)	1728
AccII	CGCG	1 cut(s)	1773
AclWI	GGATC	5 cut(s)	314, 1106, 1117, 1119, 1449
AcoI	YGGCCR	2 cut(s)	811, 1177
AcsI	RAATTY	4 cut(s)	99, 391, 482, 1696
AcuI	CTGAAG	1 cut(s)	1857
AcyI	GRCGYC	1 cut(s)	528
AfaI	GTAC	7 cut(s)	326, 653, 861, 877, 1147, 1188, 1434
AfiI	CCNNNNNNNGG	7 cut(s)	497, 568, 1162, 1182, 1346, 1360, 1394
AflII	CTTAAG	1 cut(s)	1448
AflIII	ACRYGT	2 cut(s)	580, 1771
AgsI	TTSAA	9 cut(s)	98, 596, 680, 896, 953, 1225, 1375, 1650, 1696
AhlI	ACTAGT	1 cut(s)	188
AjiI	CACGTC	2 cut(s)	23, 29
AjnI	CCWGG	2 cut(s)	643, 1345
AloI	GAACNNNNNNTCC	2 cut(s)	567, 599
Alw21I	GWGCWC	2 cut(s)	1490, 1891
Alw26I	GTCTC	3 cut(s)	594, 1762, 1840
Alw44I	GTGCAC	1 cut(s)	1486
AlwI	GGATC	5 cut(s)	314, 1106, 1117, 1119, 1449
AoxI	GGCC	3 cut(s)	242, 811, 1177
ApaLI	GTGCAC	1 cut(s)	1486
ApeKI	GCWGC	5 cut(s)	545, 842, 1277, 1456, 1475
ApoI	RAATTY	4 cut(s)	99, 391, 482, 1696
AseI	ATTAAT	1 cut(s)	1055
Asp718I	GGTACC	2 cut(s)	651, 1186
AspLEI	GCGC	7 cut(s)	530, 545, 958, 1005, 1383, 1518, 1608
AspS9I	GGNCC	4 cut(s)	242, 336, 1153, 1655
AsuC2I	CCSGG	1 cut(s)	1176
AsuHPI	GGTGA	2 cut(s)	1376, 1755
AvaII	GGWCC	3 cut(s)	336, 1153, 1655
AxyI	CCTNAGG	1 cut(s)	1023
BaeGI	GKGCMC	4 cut(s)	1038, 1176, 1490, 1558
BamHI	GGATCC	1 cut(s)	1111
BanI	GGYRCC	6 cut(s)	506, 527, 651, 1186, 1553, 1640
BanII	GRGCYC	2 cut(s)	1354, 1891
BbsI	GAAGAC	3 cut(s)	258, 583, 1232
Bbv12I	GWGCWC	2 cut(s)	1490, 1891
BbvCI	CCTCAGC	1 cut(s)	1335
BbvI	GCAGC	5 cut(s)	532, 829, 1264, 1443, 1487
BccI	CCATC	9 cut(s)	80, 244, 292, 658, 731, 932, 977, 1392, 1832
BceAI	ACGGC	1 cut(s)	514
BciT130I	CCWGG	2 cut(s)	645, 1347
BcnI	CCSGG	1 cut(s)	1176
BcoDI	GTCTC	3 cut(s)	594, 1762, 1840
BcuI	ACTAGT	1 cut(s)	188
BfaI	CTAG	4 cut(s)	189, 1106, 1496, 1788
BfoI	RGCGCY	2 cut(s)	531, 546
BfrI	CTTAAG	1 cut(s)	1448
BfuAI	ACCTGC	1 cut(s)	1541
BisI	GCNGC	9 cut(s)	227, 303, 546, 843, 1006, 1278, 1454, 1457, 1476
BlpI	GCTNAGC	1 cut(s)	705
BlsI	GCNGC	9 cut(s)	228, 304, 547, 844, 1007, 1279, 1455, 1458, 1477
Bme1390I	CCNGG	3 cut(s)	645, 1176, 1347
Bme18I	GGWCC	3 cut(s)	336, 1153, 1655
BmgBI	CACGTC	2 cut(s)	23, 29
BmgT120I	GGNCC	4 cut(s)	242, 336, 1153, 1655
BmrFI	CCNGG	3 cut(s)	645, 1176, 1347
BmsI	GCATC	8 cut(s)	87, 468, 691, 1255, 1462, 1538, 1593, 1814
BpiI	GAAGAC	3 cut(s)	258, 583, 1232
BplI	GAGNNNNNCTC	4 cut(s)	400, 432, 639, 671
BpmI	CTGGAG	3 cut(s)	623, 887, 1329
Bpu10I	CCTNAGC	2 cut(s)	1335, 1628
Bpu1102I	GCTNAGC	1 cut(s)	705
BpuEI	CTTGAG	1 cut(s)	1421
BpuMI	CCSGG	1 cut(s)	1176
Bsa29I	ATCGAT	1 cut(s)	699
BsaHI	GRCGYC	1 cut(s)	528
BsaJI	CCNNGG	4 cut(s)	567, 933, 1545, 1633
BsaWI	WCCGGW	2 cut(s)	1252, 1652
Bsc4I	CCNNNNNNNGG	7 cut(s)	497, 568, 1162, 1182, 1346, 1360, 1394
Bse118I	RCCGGY	3 cut(s)	530, 1394, 1522
Bse1I	ACTGG	2 cut(s)	870, 1658
Bse21I	CCTNAGG	1 cut(s)	1023
Bse3DI	GCAATG	3 cut(s)	418, 1194, 1215
BseBI	CCWGG	2 cut(s)	645, 1347
BseCI	ATCGAT	1 cut(s)	699
BseDI	CCNNGG	4 cut(s)	567, 933, 1545, 1633
BseGI	GGATG	8 cut(s)	58, 72, 78, 105, 742, 834, 943, 1384
BseLI	CCNNNNNNNGG	7 cut(s)	497, 568, 1162, 1182, 1346, 1360, 1394
BseMI	GCAATG	3 cut(s)	418, 1194, 1215
BseMII	CTCAG	4 cut(s)	431, 719, 1037, 1349
BseNI	ACTGG	2 cut(s)	870, 1658
BseRI	GAGGAG	5 cut(s)	55, 58, 127, 954, 1881
BseSI	GKGCMC	4 cut(s)	1038, 1176, 1490, 1558
BseXI	GCAGC	5 cut(s)	532, 829, 1264, 1443, 1487
BseYI	CCCAGC	1 cut(s)	1360
Bsh1236I	CGCG	1 cut(s)	1773
Bsh1285I	CGRYCG	1 cut(s)	444
BshFI	GGCC	3 cut(s)	244, 813, 1179
BshNI	GGYRCC	6 cut(s)	506, 527, 651, 1186, 1553, 1640
BshVI	ATCGAT	1 cut(s)	699
BsiEI	CGRYCG	1 cut(s)	444
BsiHKAI	GWGCWC	2 cut(s)	1490, 1891
BsiSI	CCGG	8 cut(s)	388, 504, 531, 1176, 1253, 1395, 1523, 1653
BsiWI	CGTACG	1 cut(s)	324
BslFI	GGGAC	2 cut(s)	871, 919
BslI	CCNNNNNNNGG	7 cut(s)	497, 568, 1162, 1182, 1346, 1360, 1394
BsmAI	GTCTC	3 cut(s)	594, 1762, 1840
BsmBI	CGTCTC	1 cut(s)	1762
BsmFI	GGGAC	2 cut(s)	871, 919
BsnI	GGCC	3 cut(s)	244, 813, 1179
Bsp1286I	GDGCHC	6 cut(s)	1038, 1176, 1354, 1490, 1558, 1891
Bsp1407I	TGTACA	1 cut(s)	859
Bsp143I	GATC	6 cut(s)	319, 441, 616, 1111, 1122, 1441
Bsp1720I	GCTNAGC	1 cut(s)	705
BspANI	GGCC	3 cut(s)	244, 813, 1179
BspCNI	CTCAG	4 cut(s)	430, 718, 1036, 1348
BspDI	ATCGAT	1 cut(s)	699
BspFNI	CGCG	1 cut(s)	1773
BspHI	TCATGA	1 cut(s)	1261
BspMI	ACCTGC	1 cut(s)	1541
BspPI	GGATC	5 cut(s)	314, 1106, 1117, 1119, 1449
BspT107I	GGYRCC	6 cut(s)	506, 527, 651, 1186, 1553, 1640
BspTI	CTTAAG	1 cut(s)	1448
BsrDI	GCAATG	3 cut(s)	418, 1194, 1215
BsrFI	RCCGGY	3 cut(s)	530, 1394, 1522
BsrGI	TGTACA	1 cut(s)	859
BsrI	ACTGG	2 cut(s)	870, 1658
BssAI	RCCGGY	3 cut(s)	530, 1394, 1522
BssECI	CCNNGG	4 cut(s)	567, 933, 1545, 1633
BssMI	GATC	6 cut(s)	319, 441, 616, 1111, 1122, 1441
BssNAI	GTATAC	1 cut(s)	1729
BssNI	GRCGYC	1 cut(s)	528
BssT1I	CCWWGG	4 cut(s)	567, 933, 1545, 1633
Bst1107I	GTATAC	1 cut(s)	1729
Bst2UI	CCWGG	2 cut(s)	645, 1347
Bst4CI	ACNGT	5 cut(s)	310, 774, 880, 1411, 1733
BstACI	GRCGYC	1 cut(s)	528
BstAFI	CTTAAG	1 cut(s)	1448
BstAPI	GCANNNNNTGC	1 cut(s)	1014
BstAUI	TGTACA	1 cut(s)	859
BstC8I	GCNNGC	8 cut(s)	532, 541, 632, 960, 1019, 1322, 1326, 1520
BstDEI	CTNAG	7 cut(s)	294, 355, 417, 705, 1023, 1335, 1628
BstENI	CCTNNNNNAGG	1 cut(s)	566
BstF5I	GGATG	8 cut(s)	58, 72, 78, 105, 742, 834, 943, 1384
BstFNI	CGCG	1 cut(s)	1773
BstH2I	RGCGCY	2 cut(s)	531, 546
BstHHI	GCGC	7 cut(s)	530, 545, 958, 1005, 1383, 1518, 1608
BstKTI	GATC	6 cut(s)	322, 444, 619, 1114, 1125, 1444
BstMAI	GTCTC	3 cut(s)	594, 1762, 1840
BstMBI	GATC	6 cut(s)	319, 441, 616, 1111, 1122, 1441
BstMCI	CGRYCG	1 cut(s)	444
BstNI	CCWGG	2 cut(s)	645, 1347
BstNSI	RCATGY	3 cut(s)	584, 1100, 1324
BstSCI	CCNGG	3 cut(s)	643, 1174, 1345
BstSLI	GKGCMC	4 cut(s)	1038, 1176, 1490, 1558
BstUI	CGCG	1 cut(s)	1773
BstV1I	GCAGC	5 cut(s)	532, 829, 1264, 1443, 1487
BstV2I	GAAGAC	3 cut(s)	258, 583, 1232
BstX2I	RGATCY	2 cut(s)	1111, 1441
BstYI	RGATCY	2 cut(s)	1111, 1441
BstZ17I	GTATAC	1 cut(s)	1729
Bsu15I	ATCGAT	1 cut(s)	699
Bsu36I	CCTNAGG	1 cut(s)	1023
BsuRI	GGCC	3 cut(s)	244, 813, 1179
BsuTUI	ATCGAT	1 cut(s)	699
BtrI	CACGTC	2 cut(s)	23, 29
BtsCI	GGATG	8 cut(s)	58, 72, 78, 105, 742, 834, 943, 1384
BtsIMutI	CAGTG	1 cut(s)	1665
BveI	ACCTGC	1 cut(s)	1541
Cac8I	GCNNGC	8 cut(s)	532, 541, 632, 960, 1019, 1322, 1326, 1520
CciI	TCATGA	1 cut(s)	1261
CfoI	GCGC	7 cut(s)	530, 545, 958, 1005, 1383, 1518, 1608
Cfr10I	RCCGGY	3 cut(s)	530, 1394, 1522
Cfr13I	GGNCC	4 cut(s)	242, 336, 1153, 1655
ClaI	ATCGAT	1 cut(s)	699
CseI	GACGC	2 cut(s)	1376, 1779
Csp6I	GTAC	7 cut(s)	325, 652, 860, 876, 1146, 1187, 1433
CviAII	CATG	8 cut(s)	39, 581, 587, 1091, 1097, 1262, 1321, 1469
CviQI	GTAC	7 cut(s)	325, 652, 860, 876, 1146, 1187, 1433
DdeI	CTNAG	7 cut(s)	294, 355, 417, 705, 1023, 1335, 1628
DinI	GGCGCC	1 cut(s)	529
DpnI	GATC	6 cut(s)	321, 443, 618, 1113, 1124, 1443
DpnII	GATC	6 cut(s)	319, 441, 616, 1111, 1122, 1441
EaeI	YGGCCR	2 cut(s)	811, 1177
EciI	GGCGGA	1 cut(s)	1413
Ecl136II	GAGCTC	1 cut(s)	1889
Eco130I	CCWWGG	4 cut(s)	567, 933, 1545, 1633
Eco24I	GRGCYC	2 cut(s)	1354, 1891
Eco47I	GGWCC	3 cut(s)	336, 1153, 1655
Eco53kI	GAGCTC	1 cut(s)	1889
Eco57I	CTGAAG	1 cut(s)	1857
Eco81I	CCTNAGG	1 cut(s)	1023
EcoICRI	GAGCTC	1 cut(s)	1889
EcoNI	CCTNNNNNAGG	1 cut(s)	566
EcoRI	GAATTC	2 cut(s)	391, 482
EcoRII	CCWGG	2 cut(s)	643, 1345
EcoT14I	CCWWGG	4 cut(s)	567, 933, 1545, 1633
EcoT38I	GRGCYC	2 cut(s)	1354, 1891
EgeI	GGCGCC	1 cut(s)	529
EheI	GGCGCC	1 cut(s)	529
ErhI	CCWWGG	4 cut(s)	567, 933, 1545, 1633
Esp3I	CGTCTC	1 cut(s)	1762
FaeI	CATG	8 cut(s)	42, 584, 590, 1094, 1100, 1265, 1324, 1472
FaqI	GGGAC	2 cut(s)	871, 919
FatI	CATG	8 cut(s)	38, 580, 586, 1090, 1096, 1261, 1320, 1468
FauI	CCCGC	2 cut(s)	212, 784
FblI	GTMKAC	1 cut(s)	1728
Fnu4HI	GCNGC	9 cut(s)	227, 303, 546, 843, 1006, 1278, 1454, 1457, 1476
FokI	GGATG	8 cut(s)	45, 59, 65, 92, 749, 841, 950, 1371
FriOI	GRGCYC	2 cut(s)	1354, 1891
Fsp4HI	GCNGC	9 cut(s)	227, 303, 546, 843, 1006, 1278, 1454, 1457, 1476
FspBI	CTAG	4 cut(s)	189, 1106, 1496, 1788
FspI	TGCGCA	1 cut(s)	1517
GlaI	GCGC	7 cut(s)	529, 544, 957, 1004, 1382, 1517, 1607
GluI	GCNGC	9 cut(s)	227, 303, 546, 843, 1006, 1278, 1454, 1457, 1476
GsaI	CCCAGC	1 cut(s)	1364
GsuI	CTGGAG	3 cut(s)	623, 887, 1329
HaeII	RGCGCY	2 cut(s)	531, 546
HaeIII	GGCC	3 cut(s)	244, 813, 1179
HapII	CCGG	8 cut(s)	388, 504, 531, 1176, 1253, 1395, 1523, 1653
HgaI	GACGC	2 cut(s)	1376, 1779
HhaI	GCGC	7 cut(s)	530, 545, 958, 1005, 1383, 1518, 1608
Hin1I	GRCGYC	1 cut(s)	528
Hin1II	CATG	8 cut(s)	42, 584, 590, 1094, 1100, 1265, 1324, 1472
Hin6I	GCGC	7 cut(s)	528, 543, 956, 1003, 1381, 1516, 1606
HinP1I	GCGC	7 cut(s)	528, 543, 956, 1003, 1381, 1516, 1606
HindIII	AAGCTT	2 cut(s)	848, 1611
HinfI	GANTC	3 cut(s)	621, 816, 1499
HpaII	CCGG	8 cut(s)	388, 504, 531, 1176, 1253, 1395, 1523, 1653
HphI	GGTGA	2 cut(s)	1376, 1755
Hpy166II	GTNNAC	4 cut(s)	26, 32, 1488, 1729
Hpy188I	TCNGA	6 cut(s)	397, 420, 472, 784, 1122, 1423
Hpy188III	TCNNGA	6 cut(s)	334, 449, 677, 767, 944, 1262
Hpy8I	GTNNAC	4 cut(s)	26, 32, 1488, 1729
Hpy99I	CGWCG	6 cut(s)	387, 442, 479, 524, 527, 629
HpyAV	CCTTC	6 cut(s)	240, 255, 262, 349, 1770, 1832
HpyCH4III	ACNGT	5 cut(s)	310, 774, 880, 1411, 1733
HpyCH4IV	ACGT	4 cut(s)	22, 28, 327, 884
HpyF3I	CTNAG	7 cut(s)	294, 355, 417, 705, 1023, 1335, 1628
HpySE526I	ACGT	4 cut(s)	22, 28, 327, 884
Hsp92I	GRCGYC	1 cut(s)	528
Hsp92II	CATG	8 cut(s)	42, 584, 590, 1094, 1100, 1265, 1324, 1472
HspAI	GCGC	7 cut(s)	528, 543, 956, 1003, 1381, 1516, 1606
KasI	GGCGCC	1 cut(s)	527
KpnI	GGTACC	2 cut(s)	655, 1190
KroI	GCCGGC	1 cut(s)	530
KroNI	GCCGGC	1 cut(s)	532
Kzo9I	GATC	6 cut(s)	319, 441, 616, 1111, 1122, 1441
LmnI	GCTCC	9 cut(s)	9, 506, 610, 868, 967, 1243, 1349, 1784, 1894
Lsp1109I	GCAGC	5 cut(s)	532, 829, 1264, 1443, 1487
LweI	GCATC	8 cut(s)	87, 468, 691, 1255, 1462, 1538, 1593, 1814
MaeI	CTAG	4 cut(s)	189, 1106, 1496, 1788
MaeII	ACGT	4 cut(s)	22, 28, 327, 884
MaeIII	GTNAC	2 cut(s)	880, 1364
MalI	GATC	6 cut(s)	321, 443, 618, 1113, 1124, 1443
MboI	GATC	6 cut(s)	319, 441, 616, 1111, 1122, 1441
MfeI	CAATTG	1 cut(s)	1281
MflI	RGATCY	2 cut(s)	1111, 1441
MhlI	GDGCHC	6 cut(s)	1038, 1176, 1354, 1490, 1558, 1891
MluI	ACGCGT	1 cut(s)	1771
Mly113I	GGCGCC	1 cut(s)	528
MmeI	TCCRAC	1 cut(s)	420
MreI	CGCCGGCG	1 cut(s)	530
MroNI	GCCGGC	1 cut(s)	530
MseI	TTAA	5 cut(s)	732, 912, 1055, 1391, 1449
MslI	CAYNNNNRTG	4 cut(s)	37, 585, 783, 1095
MspA1I	CMGCKG	2 cut(s)	1158, 1456
MspCI	CTTAAG	1 cut(s)	1448
MspI	CCGG	8 cut(s)	388, 504, 531, 1176, 1253, 1395, 1523, 1653
MspR9I	CCNGG	3 cut(s)	645, 1176, 1347
MunI	CAATTG	1 cut(s)	1281
MvaI	CCWGG	2 cut(s)	645, 1347
MvnI	CGCG	1 cut(s)	1773
NaeI	GCCGGC	1 cut(s)	532
NarI	GGCGCC	1 cut(s)	528
NciI	CCSGG	1 cut(s)	1176
NdeII	GATC	6 cut(s)	319, 441, 616, 1111, 1122, 1441
NgoMIV	GCCGGC	1 cut(s)	530
NlaIII	CATG	8 cut(s)	42, 584, 590, 1094, 1100, 1265, 1324, 1472
NmeAIII	GCCGAG	1 cut(s)	730
NmuCI	GTSAC	1 cut(s)	1364
NsbI	TGCGCA	1 cut(s)	1517
NspI	RCATGY	3 cut(s)	584, 1100, 1324
PaeI	GCATGC	1 cut(s)	1324
PagI	TCATGA	1 cut(s)	1261
PaqCI	CACCTGC	1 cut(s)	1541
PciI	ACATGT	1 cut(s)	580
PcsI	WCGNNNNNNNCGW	1 cut(s)	531
PdiI	GCCGGC	1 cut(s)	532
PfeI	GAWTC	3 cut(s)	621, 816, 1499
Pfl23II	CGTACG	1 cut(s)	324
PfoI	TCCNGGA	1 cut(s)	1345
PkrI	GCNGC	9 cut(s)	228, 304, 547, 844, 1007, 1279, 1455, 1458, 1477
Ple19I	CGATCG	1 cut(s)	444
PluTI	GGCGCC	1 cut(s)	531
PscI	ACATGT	1 cut(s)	580
PshBI	ATTAAT	1 cut(s)	1055
Psp124BI	GAGCTC	1 cut(s)	1891
Psp6I	CCWGG	2 cut(s)	643, 1345
PspFI	CCCAGC	1 cut(s)	1360
PspGI	CCWGG	2 cut(s)	643, 1345
PspLI	CGTACG	1 cut(s)	324
PspPI	GGNCC	4 cut(s)	242, 336, 1153, 1655
PsuI	RGATCY	2 cut(s)	1111, 1441
PvuI	CGATCG	1 cut(s)	444
RsaI	GTAC	7 cut(s)	326, 653, 861, 877, 1147, 1188, 1434
RsaNI	GTAC	7 cut(s)	325, 652, 860, 876, 1146, 1187, 1433
RseI	CAYNNNNRTG	4 cut(s)	37, 585, 783, 1095
SacI	GAGCTC	1 cut(s)	1891
SaqAI	TTAA	5 cut(s)	732, 912, 1055, 1391, 1449
SatI	GCNGC	9 cut(s)	227, 303, 546, 843, 1006, 1278, 1454, 1457, 1476
Sau3AI	GATC	6 cut(s)	319, 441, 616, 1111, 1122, 1441
Sau96I	GGNCC	4 cut(s)	242, 336, 1153, 1655
ScrFI	CCNGG	3 cut(s)	645, 1176, 1347
SduI	GDGCHC	6 cut(s)	1038, 1176, 1354, 1490, 1558, 1891
SfaNI	GCATC	8 cut(s)	87, 468, 691, 1255, 1462, 1538, 1593, 1814
SfoI	GGCGCC	1 cut(s)	529
SgrAI	CRCCGGYG	1 cut(s)	530
SinI	GGWCC	3 cut(s)	336, 1153, 1655
SmiMI	CAYNNNNRTG	4 cut(s)	37, 585, 783, 1095
SmlI	CTYRAG	2 cut(s)	1436, 1448
SmoI	CTYRAG	2 cut(s)	1436, 1448
SpeI	ACTAGT	1 cut(s)	188
SphI	GCATGC	1 cut(s)	1324
SspDI	GGCGCC	1 cut(s)	527
SspI	AATATT	2 cut(s)	1297, 1818
SspMI	CTAG	4 cut(s)	189, 1106, 1496, 1788
SstI	GAGCTC	1 cut(s)	1891
StyD4I	CCNGG	3 cut(s)	643, 1174, 1345
StyI	CCWWGG	4 cut(s)	567, 933, 1545, 1633
TaaI	ACNGT	5 cut(s)	310, 774, 880, 1411, 1733
TaiI	ACGT	4 cut(s)	25, 31, 330, 887
TaqI	TCGA	3 cut(s)	619, 699, 922
TatI	WGTACW	3 cut(s)	859, 1145, 1432
TauI	GCSGC	4 cut(s)	229, 305, 1008, 1456
TfiI	GAWTC	3 cut(s)	621, 816, 1499
Tru1I	TTAA	5 cut(s)	732, 912, 1055, 1391, 1449
Tru9I	TTAA	5 cut(s)	732, 912, 1055, 1391, 1449
TscAI	CASTG	1 cut(s)	1665
TseFI	GTSAC	1 cut(s)	1364
TseI	GCWGC	5 cut(s)	545, 842, 1277, 1456, 1475
Tsp45I	GTSAC	1 cut(s)	1364
TspDTI	ATGAA	6 cut(s)	204, 575, 808, 1250, 1602, 1697
TspGWI	ACGGA	6 cut(s)	312, 371, 492, 623, 1613, 1680
TspRI	CASTG	1 cut(s)	1665
Vha464I	CTTAAG	1 cut(s)	1448
VneI	GTGCAC	1 cut(s)	1486
VpaK11BI	GGWCC	3 cut(s)	336, 1153, 1655
VspI	ATTAAT	1 cut(s)	1055
XagI	CCTNNNNNAGG	1 cut(s)	566
XapI	RAATTY	4 cut(s)	99, 391, 482, 1696
XceI	RCATGY	3 cut(s)	584, 1100, 1324
XcmI	CCANNNNNNNNNTGG	2 cut(s)	862, 1207
XmiI	GTMKAC	1 cut(s)	1728
XspI	CTAG	4 cut(s)	189, 1106, 1496, 1788

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.