Rroxscaffold_5G00356060

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000108
Physical Location & Seq
Reverse (-)
35235491 .. 35238250
2760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00356060.1

Sequence Viewer

Length: 1587 bp
ATGGTGTCTTACGAGTATGTTTTGGGTGGGGTGCTAGTTTCTTTGCTGGGCTCTATTTTCTTCATGATCATCCACGCCACTCTGAATGGAAAGAAACAATTCAAGGCTTCAAGTGGAGTGAAGAGTAAAGGGTTTGAAAGGATGCCACCCAAAGGTGAGTTTCAGCCGGAAATGGAAGAGAAAAGTGCCGACGTAGTCATTGTCGGCGCCGGAGTTGCTGGTGCGGCTCTTGCTTACACTCTTGCCAAGGAAGGACGCCGCATACATGTCATTGAAAGAGACTTGAGTGAGCCTGACAGAATTGTTGGTGAGCTGTTACAGCCTGGGGGTTATCTCAAATTGCTTGAGTTGGGTCTTGAAGACTGTGCAAATGAGTCCATTGATGCTCAGAAAGTGTTTGGCTATGCTCTTTACAAAGATGGAAAGGATACAAAACTGTCTTATCCCTTGGAAAATTACAGTTCAGATGTGGCTGGTAGAAGTTTTCACAATGGGCGTTTCATTCAAAGAATGCGCGAAAAAGCTGCAAATCTATCAAATGTAAAATTGGAACAAGGGACAGTGACAACACTAATTGAAGAAAATGGTACTATCAAAGGGGTGATTTACAAGAACAAGGCTGGAGAGGAGCTAAGAACATATGCTCCACTAACAATCGTGTGTGATGGATGCTTTTCCAATCTGCGCAAATCTCTTAGTGCTCCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGGTCTTGGAGAACTGTGACCTTCCATACGCAAATCATGGTCATGTGATTTTGGGAGACCCTTCACCCATCCTATTCTATCCTATCAGTTCTACTCAGGTTCGTTGTTTGGTCGATGTACCCGGAACAAAAGTACCTTCAATAGCTAGTGGTGAAATGGTTAAATATTTGAAAACCAAGGTGGCTCCTCAGATTCCCCCTCAGCTCTACAATGCTTTTATGGCTGCTGTCAACAAAGGAAACATCAGAACAATGCAAAACAAGAGCATGGCCGCTAACCCTCTTCCCACCCCTGGTGCAATTTTACTGGGGGATTCGTTCAACATGAGGCATCCTTTAACAGGAGGAGGAATGACTGTGGCTCTTTCAGACATTGTTCTTCTCCGGAATCTTCTCAGACCCCTACGTGATCTCAATGATGCACCTGCTTTGTGCAATTACCTTGAATCATTCTACACATTGCGGAAGCCTGTGTCATCTACCATAAACACATTGGCAGGTGCTTTGTACCAGGTGTTTTGTGCATCACCTGATCCAGCAAGACAGGAAATCCGTGAAGCCTGCTTTGGTTACTTGAGCCTTGGAGGCATCTGTTCGTATGGACCCGTATCTCTTCTCTCTGGTCTTAACTCTCGCCCACTGCACTTGTTTCTCCATTTCTTTGCTGTTGCTATATATGGTGTAGGGCGCTTGATGTTTCCATTCCCTTCACCTAAAAGCATGTGGCTTGGGGCTAGATTGATATTGAGTGCATCTGGCATCCTTTTCCCCATTATAAAGGGTGAAGGAGTTAGACAGATGTTCTTTCCTGCCACTATGCCAGCATATTACAGGTCTCCTCCTCTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

528

Amino Acids

57.56

Weight (kDa)

8.96

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 63 - 376 1.1e-18 FAD binding domain
DAO PF01266 64 - 94 1.7e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 67 - 93 4.9e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 215 - 486 8.9e-116 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1511
AarI CACCTGC 2 cut(s) 1168, 1223
Acc16I TGCGCA 1 cut(s) 686
Acc36I ACCTGC 2 cut(s) 1168, 1223
AccB1I GGYRCC 1 cut(s) 206
AccII CGCG 1 cut(s) 516
AccIII TCCGGA 1 cut(s) 1119
AciI CCGC 4 cut(s) 224, 259, 1008, 1198
AclWI GGATC 1 cut(s) 1262
AcoI YGGCCR 1 cut(s) 1005
AcyI GRCGYC 2 cut(s) 207, 256
AfaI GTAC 4 cut(s) 589, 855, 870, 1244
AfiI CCNNNNNNNGG 3 cut(s) 152, 1028, 1076
AflIII ACRYGT 1 cut(s) 265
AjnI CCWGG 3 cut(s) 322, 1027, 1245
AjuI GAANNNNNNNTTGG 2 cut(s) 1284, 1316
AloI GAACNNNNNNTCC 2 cut(s) 628, 660
AluBI AGCT 5 cut(s) 313, 524, 631, 881, 940
AluI AGCT 5 cut(s) 313, 524, 631, 881, 940
Alw21I GWGCWC 1 cut(s) 703
Alw26I GTCTC 3 cut(s) 273, 786, 1575
AlwI GGATC 1 cut(s) 1262
Aor13HI TCCGGA 1 cut(s) 1119
AoxI GGCC 1 cut(s) 1005
ApeKI GCWGC 2 cut(s) 524, 959
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 4 cut(s) 209, 516, 687, 1425
AspS9I GGNCC 1 cut(s) 1337
AsuC2I CCSGG 1 cut(s) 858
AsuHPI GGTGA 8 cut(s) 167, 320, 613, 792, 899, 1254, 1437, 1529
AvaII GGWCC 1 cut(s) 1337
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 703
BbvCI CCTCAGC 1 cut(s) 936
BbvI GCAGC 2 cut(s) 511, 946
BccI CCATC 3 cut(s) 413, 659, 812
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 3 cut(s) 324, 1029, 1247
BciVI GTATCC 1 cut(s) 421
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 1 cut(s) 858
BcoDI GTCTC 3 cut(s) 273, 786, 1575
BfaI CTAG 3 cut(s) 35, 882, 1470
BfoI RGCGCY 2 cut(s) 210, 1426
BfuAI ACCTGC 2 cut(s) 1168, 1223
BfuI GTATCC 1 cut(s) 421
BglI GCCNNNNNGGC 1 cut(s) 1320
BisI GCNGC 5 cut(s) 225, 259, 525, 960, 1008
BlsI GCNGC 5 cut(s) 226, 260, 526, 961, 1009
Bme1390I CCNGG 4 cut(s) 324, 858, 1029, 1247
Bme18I GGWCC 1 cut(s) 1337
BmgT120I GGNCC 1 cut(s) 1337
BmiI GGNNCC 3 cut(s) 208, 921, 1339
BmrFI CCNGG 4 cut(s) 324, 858, 1029, 1247
BmrI ACTGGG 1 cut(s) 1052
BmsI GCATC 9 cut(s) 132, 373, 659, 1075, 1144, 1268, 1332, 1496, 1503
BmuI ACTGGG 1 cut(s) 1052
BpiI GAAGAC 1 cut(s) 366
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 936
BpuEI CTTGAG 3 cut(s) 304, 365, 1330
BpuMI CCSGG 1 cut(s) 858
BsaAI YACGTR 1 cut(s) 1142
BsaHI GRCGYC 2 cut(s) 207, 256
BsaI GGTCTC 2 cut(s) 786, 1575
BsaJI CCNNGG 6 cut(s) 246, 323, 447, 912, 1027, 1315
BsaWI WCCGGW 1 cut(s) 1119
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 1028, 1076
Bse1I ACTGG 1 cut(s) 1047
Bse3DI GCAATG 1 cut(s) 1193
BseAI TCCGGA 1 cut(s) 1119
BseBI CCWGG 3 cut(s) 324, 1029, 1247
BseDI CCNNGG 6 cut(s) 246, 323, 447, 912, 1027, 1315
BseGI GGATG 6 cut(s) 69, 147, 674, 804, 1066, 1494
BseLI CCNNNNNNNGG 3 cut(s) 152, 1028, 1076
BseMI GCAATG 1 cut(s) 1193
BseMII CTCAG 5 cut(s) 401, 845, 938, 950, 1144
BseNI ACTGG 1 cut(s) 1047
BseRI GAGGAG 5 cut(s) 641, 912, 1095, 1563, 1566
BseXI GCAGC 2 cut(s) 511, 946
BseYI CCCAGC 1 cut(s) 46
BsgI GTGCAG 1 cut(s) 1361
Bsh1236I CGCG 1 cut(s) 516
BshFI GGCC 1 cut(s) 1007
BshNI GGYRCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 703
BsiSI CCGG 4 cut(s) 167, 210, 858, 1120
BslFI GGGAC 2 cut(s) 571, 702
BslI CCNNNNNNNGG 3 cut(s) 152, 1028, 1076
BsmAI GTCTC 3 cut(s) 273, 786, 1575
BsmFI GGGAC 2 cut(s) 571, 702
BsmI GAATGC 1 cut(s) 516
BsnI GGCC 1 cut(s) 1007
Bso31I GGTCTC 2 cut(s) 786, 1575
Bsp1286I GDGCHC 2 cut(s) 53, 703
Bsp13I TCCGGA 1 cut(s) 1119
Bsp143I GATC 3 cut(s) 66, 1144, 1267
BspACI CCGC 4 cut(s) 224, 259, 1008, 1198
BspANI GGCC 1 cut(s) 1007
BspCNI CTCAG 5 cut(s) 400, 844, 937, 949, 1143
BspEI TCCGGA 1 cut(s) 1119
BspFNI CGCG 1 cut(s) 516
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 3 cut(s) 208, 921, 1339
BspMI ACCTGC 2 cut(s) 1168, 1223
BspPI GGATC 1 cut(s) 1262
BspT107I GGYRCC 1 cut(s) 206
BspTNI GGTCTC 2 cut(s) 786, 1575
BsrDI GCAATG 1 cut(s) 1193
BsrI ACTGG 1 cut(s) 1047
BssECI CCNNGG 6 cut(s) 246, 323, 447, 912, 1027, 1315
BssMI GATC 3 cut(s) 66, 1144, 1267
BssNI GRCGYC 2 cut(s) 207, 256
BssT1I CCWWGG 4 cut(s) 246, 447, 912, 1315
Bst2UI CCWGG 3 cut(s) 324, 1029, 1247
Bst4CI ACNGT 6 cut(s) 365, 438, 461, 562, 752, 1093
Bst6I CTCTTC 5 cut(s) 116, 171, 1023, 1353, 1584
BstACI GRCGYC 2 cut(s) 207, 256
BstBAI YACGTR 1 cut(s) 1142
BstC8I GCNNGC 2 cut(s) 1297, 1557
BstDEI CTNAG 7 cut(s) 387, 632, 695, 831, 924, 936, 1130
BstENI CCTNNNNNAGG 1 cut(s) 1074
BstF5I GGATG 6 cut(s) 69, 147, 674, 804, 1066, 1494
BstFNI CGCG 1 cut(s) 516
BstH2I RGCGCY 2 cut(s) 210, 1426
BstHHI GCGC 4 cut(s) 209, 516, 687, 1425
BstKTI GATC 3 cut(s) 69, 1147, 1270
BstMAI GTCTC 3 cut(s) 273, 786, 1575
BstMBI GATC 3 cut(s) 66, 1144, 1267
BstMWI GCNNNNNNNGC 6 cut(s) 215, 224, 230, 319, 956, 1320
BstNI CCWGG 3 cut(s) 324, 1029, 1247
BstNSI RCATGY 2 cut(s) 269, 1459
BstSCI CCNGG 4 cut(s) 322, 856, 1027, 1245
BstUI CGCG 1 cut(s) 516
BstV1I GCAGC 2 cut(s) 511, 946
BstV2I GAAGAC 1 cut(s) 366
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 1 cut(s) 1007
BtsCI GGATG 6 cut(s) 69, 147, 674, 804, 1066, 1494
BtsI GCAGTG 1 cut(s) 1373
BtsIMutI CAGTG 2 cut(s) 567, 1373
BveI ACCTGC 2 cut(s) 1168, 1223
Cac8I GCNNGC 2 cut(s) 1297, 1557
CciI TCATGA 1 cut(s) 63
CfoI GCGC 4 cut(s) 209, 516, 687, 1425
Cfr13I GGNCC 1 cut(s) 1337
CseI GACGC 1 cut(s) 264
CsiI ACCWGGT 1 cut(s) 1245
Csp6I GTAC 4 cut(s) 588, 854, 869, 1243
CspCI CAANNNNNGTGG 2 cut(s) 1362, 1397
CviAII CATG 7 cut(s) 64, 266, 773, 779, 1003, 1060, 1456
CviQI GTAC 4 cut(s) 588, 854, 869, 1243
DdeI CTNAG 7 cut(s) 387, 632, 695, 831, 924, 936, 1130
DinI GGCGCC 1 cut(s) 208
DpnI GATC 3 cut(s) 68, 1146, 1269
DpnII GATC 3 cut(s) 66, 1144, 1267
EaeI YGGCCR 1 cut(s) 1005
Eam1104I CTCTTC 5 cut(s) 116, 171, 1023, 1353, 1584
EarI CTCTTC 5 cut(s) 116, 171, 1023, 1353, 1584
Eco130I CCWWGG 4 cut(s) 246, 447, 912, 1315
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 2 cut(s) 786, 1575
Eco47I GGWCC 1 cut(s) 1337
EcoNI CCTNNNNNAGG 1 cut(s) 1074
EcoRII CCWGG 3 cut(s) 322, 1027, 1245
EcoT14I CCWWGG 4 cut(s) 246, 447, 912, 1315
EcoT38I GRGCYC 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 208
EheI GGCGCC 1 cut(s) 208
ErhI CCWWGG 4 cut(s) 246, 447, 912, 1315
FaeI CATG 7 cut(s) 67, 269, 776, 782, 1006, 1063, 1459
FalI AAGNNNNNCTT 2 cut(s) 706, 738
FaqI GGGAC 2 cut(s) 571, 702
FatI CATG 7 cut(s) 63, 265, 772, 778, 1002, 1059, 1455
FauNDI CATATG 1 cut(s) 640
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 5 cut(s) 225, 259, 525, 960, 1008
FokI GGATG 6 cut(s) 56, 154, 681, 791, 1053, 1481
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 5 cut(s) 225, 259, 525, 960, 1008
FspBI CTAG 3 cut(s) 35, 882, 1470
FspI TGCGCA 1 cut(s) 686
GlaI GCGC 4 cut(s) 208, 515, 686, 1424
GluI GCNGC 5 cut(s) 225, 259, 525, 960, 1008
GsaI CCCAGC 1 cut(s) 50
GsuI CTGGAG 1 cut(s) 642
HaeII RGCGCY 2 cut(s) 210, 1426
HaeIII GGCC 1 cut(s) 1007
HapII CCGG 4 cut(s) 167, 210, 858, 1120
HgaI GACGC 1 cut(s) 264
HhaI GCGC 4 cut(s) 209, 516, 687, 1425
Hin1I GRCGYC 2 cut(s) 207, 256
Hin1II CATG 7 cut(s) 67, 269, 776, 782, 1006, 1063, 1459
Hin6I GCGC 4 cut(s) 207, 514, 685, 1423
HinP1I GCGC 4 cut(s) 207, 514, 685, 1423
HincII GTYRAC 1 cut(s) 967
HindII GTYRAC 1 cut(s) 967
HinfI GANTC 5 cut(s) 374, 928, 1049, 1123, 1181
HpaII CCGG 4 cut(s) 167, 210, 858, 1120
HphI GGTGA 8 cut(s) 167, 320, 613, 792, 899, 1254, 1437, 1529
Hpy166II GTNNAC 1 cut(s) 967
Hpy188I TCNGA 7 cut(s) 84, 390, 466, 927, 983, 1105, 1133
Hpy188III TCNNGA 3 cut(s) 64, 356, 1120
Hpy8I GTNNAC 1 cut(s) 967
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 6 cut(s) 245, 767, 807, 882, 1452, 1514
HpyCH4III ACNGT 6 cut(s) 365, 438, 461, 562, 752, 1093
HpyCH4IV ACGT 2 cut(s) 192, 1141
HpyCH4V TGCA 9 cut(s) 368, 527, 991, 1034, 1157, 1170, 1259, 1378, 1487
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 224, 230, 319, 956, 1320
HpyF3I CTNAG 7 cut(s) 387, 632, 695, 831, 924, 936, 1130
HpySE526I ACGT 2 cut(s) 192, 1141
Hsp92I GRCGYC 2 cut(s) 207, 256
Hsp92II CATG 7 cut(s) 67, 269, 776, 782, 1006, 1063, 1459
HspAI GCGC 4 cut(s) 207, 514, 685, 1423
KasI GGCGCC 1 cut(s) 206
Kpn2I TCCGGA 1 cut(s) 1119
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 3 cut(s) 66, 1144, 1267
LmnI GCTCC 4 cut(s) 628, 649, 706, 925
Lsp1109I GCAGC 2 cut(s) 511, 946
LweI GCATC 9 cut(s) 132, 373, 659, 1075, 1144, 1268, 1332, 1496, 1503
MabI ACCWGGT 1 cut(s) 1245
MaeI CTAG 3 cut(s) 35, 882, 1470
MaeII ACGT 2 cut(s) 192, 1141
MaeIII GTNAC 4 cut(s) 315, 562, 752, 1304
MalI GATC 3 cut(s) 68, 1146, 1269
MboI GATC 3 cut(s) 66, 1144, 1267
MhlI GDGCHC 2 cut(s) 53, 703
MluCI AATT 8 cut(s) 98, 300, 338, 454, 545, 573, 1035, 1171
Mly113I GGCGCC 1 cut(s) 207
MlyI GAGTC 1 cut(s) 383
MroI TCCGGA 1 cut(s) 1119
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 3 cut(s) 897, 1073, 1362
MslI CAYNNNNRTG 1 cut(s) 777
MspI CCGG 4 cut(s) 167, 210, 858, 1120
MspR9I CCNGG 4 cut(s) 324, 858, 1029, 1247
Mva1269I GAATGC 1 cut(s) 516
MvaI CCWGG 3 cut(s) 324, 1029, 1247
MvnI CGCG 1 cut(s) 516
MwoI GCNNNNNNNGC 6 cut(s) 215, 224, 230, 319, 956, 1320
NarI GGCGCC 1 cut(s) 207
NciI CCSGG 1 cut(s) 858
NdeI CATATG 1 cut(s) 640
NdeII GATC 3 cut(s) 66, 1144, 1267
NlaIII CATG 7 cut(s) 67, 269, 776, 782, 1006, 1063, 1459
NlaIV GGNNCC 3 cut(s) 208, 921, 1339
NmuCI GTSAC 2 cut(s) 562, 752
NsbI TGCGCA 1 cut(s) 686
NspI RCATGY 2 cut(s) 269, 1459
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 2 cut(s) 1168, 1223
PciI ACATGT 1 cut(s) 265
PctI GAATGC 1 cut(s) 516
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 4 cut(s) 928, 1049, 1123, 1181
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 5 cut(s) 226, 260, 526, 961, 1009
PleI GAGTC 1 cut(s) 382
PluTI GGCGCC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 382
Ppu21I YACGTR 1 cut(s) 1142
PscI ACATGT 1 cut(s) 265
PsiI TTATAA 1 cut(s) 1511
Psp6I CCWGG 3 cut(s) 322, 1027, 1245
PspFI CCCAGC 1 cut(s) 46
PspGI CCWGG 3 cut(s) 322, 1027, 1245
PspN4I GGNNCC 3 cut(s) 208, 921, 1339
PspPI GGNCC 1 cut(s) 1337
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 4 cut(s) 589, 855, 870, 1244
RsaNI GTAC 4 cut(s) 588, 854, 869, 1243
RseI CAYNNNNRTG 1 cut(s) 777
SaqAI TTAA 3 cut(s) 897, 1073, 1362
SatI GCNGC 5 cut(s) 225, 259, 525, 960, 1008
Sau3AI GATC 3 cut(s) 66, 1144, 1267
Sau96I GGNCC 1 cut(s) 1337
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 4 cut(s) 324, 858, 1029, 1247
SduI GDGCHC 2 cut(s) 53, 703
SexAI ACCWGGT 1 cut(s) 1245
SfaNI GCATC 9 cut(s) 132, 373, 659, 1075, 1144, 1268, 1332, 1496, 1503
SfoI GGCGCC 1 cut(s) 208
SinI GGWCC 1 cut(s) 1337
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 3 cut(s) 283, 344, 1309
SmoI CTYRAG 3 cut(s) 283, 344, 1309
Sse9I AATT 8 cut(s) 98, 300, 338, 454, 545, 573, 1035, 1171
SsiI CCGC 4 cut(s) 224, 259, 1008, 1198
SspDI GGCGCC 1 cut(s) 206
SspI AATATT 1 cut(s) 902
SspMI CTAG 3 cut(s) 35, 882, 1470
StyD4I CCNGG 4 cut(s) 322, 856, 1027, 1245
StyI CCWWGG 4 cut(s) 246, 447, 912, 1315
TaaI ACNGT 6 cut(s) 365, 438, 461, 562, 752, 1093
TaiI ACGT 2 cut(s) 195, 1144
TaqI TCGA 1 cut(s) 849
TasI AATT 8 cut(s) 98, 300, 338, 454, 545, 573, 1035, 1171
TauI GCSGC 3 cut(s) 227, 261, 1010
TfiI GAWTC 4 cut(s) 928, 1049, 1123, 1181
Tru1I TTAA 3 cut(s) 897, 1073, 1362
Tru9I TTAA 3 cut(s) 897, 1073, 1362
TscAI CASTG 2 cut(s) 567, 1380
TseFI GTSAC 2 cut(s) 562, 752
TseI GCWGC 2 cut(s) 524, 959
Tsp45I GTSAC 2 cut(s) 562, 752
TspDTI ATGAA 3 cut(s) 52, 490, 1571
TspGWI ACGGA 1 cut(s) 1277
TspRI CASTG 2 cut(s) 567, 1380
Tth111I GACNNNGTC 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 1337
XagI CCTNNNNNAGG 1 cut(s) 1074
XceI RCATGY 2 cut(s) 269, 1459
XcmI CCANNNNNNNNNTGG 1 cut(s) 1225
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 3 cut(s) 35, 882, 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.