RchiOBHm_Chr6g0246981

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000108
Physical Location & Seq
Forward (+)
2750356 .. 2755606
5251 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22139

Sequence Viewer

Length: 1758 bp
ATGGTTTCTTACGAGTATATTCTGGGTGGTTTTTTAGCTTCATTGCTGGGCTCTGTTTTCTTCATGATCATAAGTACTACTCTTAGTGGAAACAAAAAGGTTAAGGCTTTAAGTGGAGGCGTCAAGAGTAATGCGTTTGGAGACGGTGCGTTTCGGTCGGAAATGGAGGAGGAGAAAAGCACTGACTTCGTCATTGTCGGTGCCGGAGTTGCCGGTGCAGCTCTTGTTTACACTCTTGCCAAGATGGTTTCTTACGAGTATATTCTGGGTGGGTTGTTAGCTTCATTGCTGGGCTCTGTTTTCTTCATGATCATCAGTACTACACTCGGCGGAAACAAAAAGGTCAAGGCTTTAAGTGGGGGAGTCAAGAGTAATGCGTTTGGAGACGGTGCGTTTCTGCCGGAAATGGAAGAGGAGAAAAGTACCGACGTCGTTGTTGTCGGTGCCGGAGAAGGACGTCGTGTACATGTCATAGGAAGAGACTTGACTGAGCCAGATACAATTGTTGGTGAGTTGTTGCAGCCTGGGGGTTATCTCAAATTGATTGAGTTGGGTCTTGAGGACTGTGCAAATGAGTCTATTGATGCTCAGAAGGTGTTTGGTTATGCTCTGTACAAAGATGGGAAGGATACTACACTGACTTATCCCTTGGAAAAATATAGTTCAGATGTCGCTGGGAGGAGTTTCCACAATGGTCGTTTCATTCAGAGAATGCGTGAAAAATTGGAACAAGGATCAGTGACAAAACTAATCGAGGAAAAGGGCATTGTCAAACGGGTGATTTACAAGAACAAGGCTGGAGAGGAGATGAGAACATATGCTCCACTAACAATTGTTGAAAGCCCCTCCTGTTTTGTTGGTTTGATCTTGGAGAACTGTGAGCTTCCACACGCAAATCATGGACACGTAATTTTGGGAGACCCTTCACCCATCCTGTTTTATCCCATCAGTTCCACTGAGGTTCGTTGTTTGGTCAATATACCTGGAACAAAAGTACCTTCAGTAGCTAATGGTGAAATGGTCAACTATCTGAAGACTGTAGTGGCTCCTCAGGTTCCCTCACAGCTGTACAATGCCTTTATGGCTGCTGTCAACAAGGGAAACATCAGATCCATGCAAAACAAAAGCATGGCTGCTAATCCTCTTCCCACTCCTGGTGCCATTTTATTGGGGGACTCATTCAACATGAGGCATCTTTTAACCGGAGGTGGCATGACTGTGGCTCTTTCAGACATAGTTCTTCTCCGTGATCTTCTTAGACACCTACATGATCTCAATGTTGCACCTGCTCTGTGCAATTATCTTGAATCATTTTATACGTTGCTTAAGCCTGTGTCATCAACCATAAACACATTGGCAGGTGCTTTGTACAAGGTGTTTTGTGCATCACCTGATCCAGCAAGACAGGAAATGCGTGAAGCATGTTTTGGCTATTTGAGCCTTGGAGGAGTCTGTTCATATGGACCAGTATCGCTTCTCTCTGGTCTTAACCCTCGTCCACTACACTTGTTTCTCCATTTCTTTATTGTTGCCATCTATGGCGTCGGCCGCTTAATGCTTCCAATCCCTTCACCTAAATGCATGTGGCTTGGGTTTCAATTGATCTTGCCTCTAACGTGGTTCCGCCACTGTGCACATAACTGGCTGCTCATACAAATCTTTCTGGTATTTCCGAGCGCATCAGGCATCATATTCCACATTATAAAGGGCGAAGGAGTTAGAAAGATGTTTTTCCCTGCCATAGTACCAGCATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

585

Amino Acids

63.56

Weight (kDa)

8.19

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SE PF08491 280 - 524 1.4e-97 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1703
AarI CACCTGC 2 cut(s) 1294, 1349
AatII GACGTC 2 cut(s) 432, 460
Acc36I ACCTGC 2 cut(s) 1294, 1349
AccB1I GGYRCC 3 cut(s) 200, 443, 1157
AciI CCGC 3 cut(s) 330, 1549, 1624
AclWI GGATC 3 cut(s) 742, 1104, 1388
AcoI YGGCCR 1 cut(s) 1546
AcuI CTGAAG 2 cut(s) 984, 1052
AcyI GRCGYC 4 cut(s) 120, 429, 457, 1542
AfaI GTAC 9 cut(s) 76, 319, 424, 465, 614, 996, 1070, 1370, 1746
AfiI CCNNNNNNNGG 1 cut(s) 1154
AflII CTTAAG 1 cut(s) 1325
AflIII ACRYGT 2 cut(s) 466, 904
AgsI TTSAA 4 cut(s) 839, 1183, 1307, 1598
AjnI CCWGG 3 cut(s) 523, 982, 1153
AjuI GAANNNNNNNTTGG 2 cut(s) 1410, 1442
AloI GAACNNNNNNTCC 2 cut(s) 805, 837
AluBI AGCT 6 cut(s) 38, 221, 281, 883, 1007, 1066
AluI AGCT 6 cut(s) 38, 221, 281, 883, 1007, 1066
Alw21I GWGCWC 1 cut(s) 1636
Alw26I GTCTC 4 cut(s) 135, 378, 474, 912
Alw44I GTGCAC 1 cut(s) 1632
AlwI GGATC 3 cut(s) 742, 1104, 1388
AoxI GGCC 1 cut(s) 1546
ApaLI GTGCAC 1 cut(s) 1632
ApeKI GCWGC 5 cut(s) 218, 520, 1085, 1133, 1645
AspLEI GCGC 1 cut(s) 1679
AspS9I GGNCC 1 cut(s) 1463
AsuHPI GGTGA 6 cut(s) 521, 790, 918, 1025, 1380, 1563
AvaII GGWCC 1 cut(s) 1463
AxyI CCTNAGG 1 cut(s) 1050
BaeGI GKGCMC 1 cut(s) 1636
BaeI ACNNNNGTAYC 4 cut(s) 489, 522, 978, 1011
BanI GGYRCC 3 cut(s) 200, 443, 1157
BanII GRGCYC 2 cut(s) 53, 296
BbsI GAAGAC 1 cut(s) 1040
Bbv12I GWGCWC 1 cut(s) 1636
BbvI GCAGC 5 cut(s) 230, 532, 1072, 1120, 1632
BccI CCATC 5 cut(s) 238, 614, 938, 953, 1541
BcgI CGANNNNNNTGC 2 cut(s) 169, 203
BciT130I CCWGG 3 cut(s) 525, 984, 1155
BciVI GTATCC 1 cut(s) 622
BclI TGATCA 2 cut(s) 66, 309
BcoDI GTCTC 4 cut(s) 135, 378, 474, 912
BfmI CTRYAG 1 cut(s) 1038
BfrI CTTAAG 1 cut(s) 1325
BfuAI ACCTGC 2 cut(s) 1294, 1349
BfuI GTATCC 1 cut(s) 622
BglI GCCNNNNNGGC 1 cut(s) 1082
BisI GCNGC 6 cut(s) 219, 521, 1086, 1134, 1549, 1646
BlsI GCNGC 6 cut(s) 220, 522, 1087, 1135, 1550, 1647
BmcAI AGTACT 2 cut(s) 76, 319
Bme1390I CCNGG 3 cut(s) 525, 984, 1155
Bme18I GGWCC 1 cut(s) 1463
BmgT120I GGNCC 1 cut(s) 1463
BmiI GGNNCC 6 cut(s) 202, 445, 1047, 1056, 1159, 1622
BmrFI CCNGG 3 cut(s) 525, 984, 1155
BmsI GCATC 5 cut(s) 574, 1201, 1394, 1688, 1695
BpiI GAAGAC 1 cut(s) 1040
BpmI CTGGAG 1 cut(s) 819
BpuEI CTTGAG 1 cut(s) 578
BsaAI YACGTR 1 cut(s) 907
BsaHI GRCGYC 4 cut(s) 120, 429, 457, 1542
BsaI GGTCTC 1 cut(s) 912
BsaJI CCNNGG 3 cut(s) 524, 648, 1441
BsaWI WCCGGW 1 cut(s) 1202
BsaXI ACNNNNNCTCC 2 cut(s) 805, 835
Bsc4I CCNNNNNNNGG 1 cut(s) 1154
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 1466, 1646
Bse21I CCTNAGG 1 cut(s) 1050
Bse3DI GCAATG 2 cut(s) 41, 284
BseBI CCWGG 3 cut(s) 525, 984, 1155
BseDI CCNNGG 3 cut(s) 524, 648, 1441
BseGI GGATG 1 cut(s) 930
BseLI CCNNNNNNNGG 1 cut(s) 1154
BseMI GCAATG 2 cut(s) 41, 284
BseMII CTCAG 4 cut(s) 480, 602, 948, 1064
BseNI ACTGG 2 cut(s) 1466, 1646
BseRI GAGGAG 7 cut(s) 182, 185, 428, 694, 818, 1038, 1461
BseSI GKGCMC 1 cut(s) 1636
BseX3I CGGCCG 1 cut(s) 1546
BseXI GCAGC 5 cut(s) 230, 532, 1072, 1120, 1632
BseYI CCCAGC 3 cut(s) 46, 289, 674
BsgI GTGCAG 1 cut(s) 237
Bsh1285I CGRYCG 2 cut(s) 158, 1549
BshFI GGCC 1 cut(s) 1548
BshNI GGYRCC 3 cut(s) 200, 443, 1157
BsiEI CGRYCG 2 cut(s) 158, 1549
BsiHKAI GWGCWC 1 cut(s) 1636
BsiSI CCGG 5 cut(s) 204, 213, 401, 447, 1203
BslFI GGGAC 1 cut(s) 1187
BslI CCNNNNNNNGG 1 cut(s) 1154
BsmAI GTCTC 4 cut(s) 135, 378, 474, 912
BsmBI CGTCTC 2 cut(s) 135, 378
BsmFI GGGAC 1 cut(s) 1187
BsmI GAATGC 1 cut(s) 717
BsnI GGCC 1 cut(s) 1548
Bso31I GGTCTC 1 cut(s) 912
Bsp1286I GDGCHC 3 cut(s) 53, 296, 1636
Bsp1407I TGTACA 4 cut(s) 463, 612, 1068, 1368
Bsp143I GATC 9 cut(s) 66, 309, 734, 864, 1109, 1249, 1270, 1393, 1602
BspACI CCGC 3 cut(s) 330, 1549, 1624
BspANI GGCC 1 cut(s) 1548
BspCNI CTCAG 4 cut(s) 481, 601, 949, 1063
BspHI TCATGA 2 cut(s) 63, 306
BspLI GGNNCC 6 cut(s) 202, 445, 1047, 1056, 1159, 1622
BspMI ACCTGC 2 cut(s) 1294, 1349
BspPI GGATC 3 cut(s) 742, 1104, 1388
BspT107I GGYRCC 3 cut(s) 200, 443, 1157
BspTI CTTAAG 1 cut(s) 1325
BspTNI GGTCTC 1 cut(s) 912
BsrDI GCAATG 2 cut(s) 41, 284
BsrFI RCCGGY 1 cut(s) 212
BsrGI TGTACA 4 cut(s) 463, 612, 1068, 1368
BsrI ACTGG 2 cut(s) 1466, 1646
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 3 cut(s) 524, 648, 1441
BssMI GATC 9 cut(s) 66, 309, 734, 864, 1109, 1249, 1270, 1393, 1602
BssNI GRCGYC 4 cut(s) 120, 429, 457, 1542
BssT1I CCWWGG 2 cut(s) 648, 1441
Bst2UI CCWGG 3 cut(s) 525, 984, 1155
Bst4CI ACNGT 7 cut(s) 146, 389, 566, 878, 1039, 1219, 1631
Bst6I CTCTTC 3 cut(s) 405, 472, 1149
BstACI GRCGYC 4 cut(s) 120, 429, 457, 1542
BstAFI CTTAAG 1 cut(s) 1325
BstAUI TGTACA 4 cut(s) 463, 612, 1068, 1368
BstBAI YACGTR 1 cut(s) 907
BstDEI CTNAG 6 cut(s) 83, 489, 588, 957, 1050, 1256
BstF5I GGATG 1 cut(s) 930
BstHHI GCGC 1 cut(s) 1679
BstKTI GATC 9 cut(s) 69, 312, 737, 867, 1112, 1252, 1273, 1396, 1605
BstMAI GTCTC 4 cut(s) 135, 378, 474, 912
BstMBI GATC 9 cut(s) 66, 309, 734, 864, 1109, 1249, 1270, 1393, 1602
BstMCI CGRYCG 2 cut(s) 158, 1549
BstMWI GCNNNNNNNGC 6 cut(s) 209, 218, 1082, 1437, 1548, 1683
BstNI CCWGG 3 cut(s) 525, 984, 1155
BstNSI RCATGY 3 cut(s) 470, 1425, 1585
BstSCI CCNGG 3 cut(s) 523, 982, 1153
BstSFI CTRYAG 1 cut(s) 1038
BstSLI GKGCMC 1 cut(s) 1636
BstV1I GCAGC 5 cut(s) 230, 532, 1072, 1120, 1632
BstV2I GAAGAC 1 cut(s) 1040
BstX2I RGATCY 1 cut(s) 1109
BstXI CCANNNNNNTGG 1 cut(s) 1168
BstYI RGATCY 1 cut(s) 1109
BstZI CGGCCG 1 cut(s) 1546
Bsu36I CCTNAGG 1 cut(s) 1050
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 1 cut(s) 1548
BtsCI GGATG 1 cut(s) 930
BtsIMutI CAGTG 5 cut(s) 180, 635, 744, 954, 1627
BveI ACCTGC 2 cut(s) 1294, 1349
CciI TCATGA 2 cut(s) 63, 306
CfoI GCGC 1 cut(s) 1679
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 1463
CseI GACGC 2 cut(s) 109, 1531
Csp6I GTAC 9 cut(s) 75, 318, 423, 464, 613, 995, 1069, 1369, 1745
CspCI CAANNNNNGTGG 2 cut(s) 1488, 1523
CviQI GTAC 9 cut(s) 75, 318, 423, 464, 613, 995, 1069, 1369, 1745
DdeI CTNAG 6 cut(s) 83, 489, 588, 957, 1050, 1256
DpnI GATC 9 cut(s) 68, 311, 736, 866, 1111, 1251, 1272, 1395, 1604
DpnII GATC 9 cut(s) 66, 309, 734, 864, 1109, 1249, 1270, 1393, 1602
EaeI YGGCCR 1 cut(s) 1546
EagI CGGCCG 1 cut(s) 1546
Eam1104I CTCTTC 3 cut(s) 405, 472, 1149
EarI CTCTTC 3 cut(s) 405, 472, 1149
EciI GGCGGA 2 cut(s) 345, 1613
EclXI CGGCCG 1 cut(s) 1546
Eco130I CCWWGG 2 cut(s) 648, 1441
Eco24I GRGCYC 2 cut(s) 53, 296
Eco31I GGTCTC 1 cut(s) 912
Eco47I GGWCC 1 cut(s) 1463
Eco52I CGGCCG 1 cut(s) 1546
Eco57I CTGAAG 2 cut(s) 984, 1052
Eco81I CCTNAGG 1 cut(s) 1050
EcoRII CCWGG 3 cut(s) 523, 982, 1153
EcoT14I CCWWGG 2 cut(s) 648, 1441
EcoT22I ATGCAT 1 cut(s) 1583
EcoT38I GRGCYC 2 cut(s) 53, 296
ErhI CCWWGG 2 cut(s) 648, 1441
Esp3I CGTCTC 2 cut(s) 135, 378
FaqI GGGAC 1 cut(s) 1187
FauNDI CATATG 2 cut(s) 817, 1459
FbaI TGATCA 2 cut(s) 66, 309
Fnu4HI GCNGC 6 cut(s) 219, 521, 1086, 1134, 1549, 1646
FokI GGATG 1 cut(s) 917
FriOI GRGCYC 2 cut(s) 53, 296
Fsp4HI GCNGC 6 cut(s) 219, 521, 1086, 1134, 1549, 1646
GlaI GCGC 1 cut(s) 1678
GluI GCNGC 6 cut(s) 219, 521, 1086, 1134, 1549, 1646
GsaI CCCAGC 3 cut(s) 50, 293, 678
GsuI CTGGAG 1 cut(s) 819
HaeIII GGCC 1 cut(s) 1548
HapII CCGG 5 cut(s) 204, 213, 401, 447, 1203
HgaI GACGC 2 cut(s) 109, 1531
HhaI GCGC 1 cut(s) 1679
Hin1I GRCGYC 4 cut(s) 120, 429, 457, 1542
Hin6I GCGC 1 cut(s) 1677
HinP1I GCGC 1 cut(s) 1677
HincII GTYRAC 2 cut(s) 1024, 1093
HindII GTYRAC 2 cut(s) 1024, 1093
HinfI GANTC 5 cut(s) 363, 575, 1175, 1307, 1449
HpaII CCGG 5 cut(s) 204, 213, 401, 447, 1203
HphI GGTGA 6 cut(s) 521, 790, 918, 1025, 1380, 1563
Hpy166II GTNNAC 6 cut(s) 229, 464, 1024, 1093, 1499, 1634
Hpy188I TCNGA 8 cut(s) 160, 591, 667, 708, 1032, 1109, 1231, 1674
Hpy188III TCNNGA 6 cut(s) 64, 124, 307, 367, 557, 1304
Hpy8I GTNNAC 6 cut(s) 229, 464, 1024, 1093, 1499, 1634
Hpy99I CGWCG 4 cut(s) 431, 434, 462, 1547
HpyAV CCTTC 7 cut(s) 446, 586, 619, 933, 1008, 1578, 1706
HpyCH4III ACNGT 7 cut(s) 146, 389, 566, 878, 1039, 1219, 1631
HpyCH4IV ACGT 5 cut(s) 429, 457, 906, 1319, 1616
HpyCH4V TGCA 9 cut(s) 218, 520, 569, 1117, 1283, 1296, 1385, 1581, 1634
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 218, 1082, 1437, 1548, 1683
HpyF3I CTNAG 6 cut(s) 83, 489, 588, 957, 1050, 1256
HpySE526I ACGT 5 cut(s) 429, 457, 906, 1319, 1616
Hsp92I GRCGYC 4 cut(s) 120, 429, 457, 1542
HspAI GCGC 1 cut(s) 1677
Ksp22I TGATCA 2 cut(s) 66, 309
Kzo9I GATC 9 cut(s) 66, 309, 734, 864, 1109, 1249, 1270, 1393, 1602
LmnI GCTCC 2 cut(s) 826, 1051
Lsp1109I GCAGC 5 cut(s) 230, 532, 1072, 1120, 1632
LweI GCATC 5 cut(s) 574, 1201, 1394, 1688, 1695
MaeII ACGT 5 cut(s) 429, 457, 906, 1319, 1616
MaeIII GTNAC 1 cut(s) 739
MalI GATC 9 cut(s) 68, 311, 736, 866, 1111, 1251, 1272, 1395, 1604
MboI GATC 9 cut(s) 66, 309, 734, 864, 1109, 1249, 1270, 1393, 1602
MboII GAAGA 8 cut(s) 52, 295, 422, 489, 1045, 1136, 1232, 1244
MfeI CAATTG 3 cut(s) 501, 831, 1598
MflI RGATCY 1 cut(s) 1109
MhlI GDGCHC 3 cut(s) 53, 296, 1636
MluCI AATT 7 cut(s) 501, 539, 722, 831, 909, 1297, 1598
MlyI GAGTC 4 cut(s) 372, 584, 1169, 1458
MmeI TCCRAC 1 cut(s) 138
Mph1103I ATGCAT 1 cut(s) 1583
MseI TTAA 7 cut(s) 102, 110, 353, 1199, 1326, 1488, 1553
MslI CAYNNNNRTG 3 cut(s) 1217, 1266, 1576
MspA1I CMGCKG 1 cut(s) 1066
MspCI CTTAAG 1 cut(s) 1325
MspI CCGG 5 cut(s) 204, 213, 401, 447, 1203
MspR9I CCNGG 3 cut(s) 525, 984, 1155
MunI CAATTG 3 cut(s) 501, 831, 1598
Mva1269I GAATGC 1 cut(s) 717
MvaI CCWGG 3 cut(s) 525, 984, 1155
MwoI GCNNNNNNNGC 6 cut(s) 209, 218, 1082, 1437, 1548, 1683
NdeI CATATG 2 cut(s) 817, 1459
NdeII GATC 9 cut(s) 66, 309, 734, 864, 1109, 1249, 1270, 1393, 1602
NlaIV GGNNCC 6 cut(s) 202, 445, 1047, 1056, 1159, 1622
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 1 cut(s) 739
NsiI ATGCAT 1 cut(s) 1583
NspI RCATGY 3 cut(s) 470, 1425, 1585
PagI TCATGA 2 cut(s) 63, 306
PaqCI CACCTGC 2 cut(s) 1294, 1349
PciI ACATGT 1 cut(s) 466
PctI GAATGC 1 cut(s) 717
PfeI GAWTC 1 cut(s) 1307
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 6 cut(s) 220, 522, 1087, 1135, 1550, 1647
PleI GAGTC 4 cut(s) 371, 583, 1169, 1457
PpsI GAGTC 4 cut(s) 371, 583, 1169, 1457
Ppu21I YACGTR 1 cut(s) 907
PscI ACATGT 1 cut(s) 466
PsiI TTATAA 1 cut(s) 1703
Psp6I CCWGG 3 cut(s) 523, 982, 1153
PspFI CCCAGC 3 cut(s) 46, 289, 674
PspGI CCWGG 3 cut(s) 523, 982, 1153
PspN4I GGNNCC 6 cut(s) 202, 445, 1047, 1056, 1159, 1622
PspPI GGNCC 1 cut(s) 1463
PsuI RGATCY 1 cut(s) 1109
PsyI GACNNNGTC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 1066
RsaI GTAC 9 cut(s) 76, 319, 424, 465, 614, 996, 1070, 1370, 1746
RsaNI GTAC 9 cut(s) 75, 318, 423, 464, 613, 995, 1069, 1369, 1745
RseI CAYNNNNRTG 3 cut(s) 1217, 1266, 1576
SaqAI TTAA 7 cut(s) 102, 110, 353, 1199, 1326, 1488, 1553
SatI GCNGC 6 cut(s) 219, 521, 1086, 1134, 1549, 1646
Sau3AI GATC 9 cut(s) 66, 309, 734, 864, 1109, 1249, 1270, 1393, 1602
Sau96I GGNCC 1 cut(s) 1463
ScaI AGTACT 2 cut(s) 76, 319
SchI GAGTC 4 cut(s) 372, 584, 1169, 1458
ScrFI CCNGG 3 cut(s) 525, 984, 1155
SduI GDGCHC 3 cut(s) 53, 296, 1636
SfaNI GCATC 5 cut(s) 574, 1201, 1394, 1688, 1695
SfcI CTRYAG 1 cut(s) 1038
SinI GGWCC 1 cut(s) 1463
SmiMI CAYNNNNRTG 3 cut(s) 1217, 1266, 1576
SmlI CTYRAG 2 cut(s) 557, 1325
SmoI CTYRAG 2 cut(s) 557, 1325
Sse9I AATT 7 cut(s) 501, 539, 722, 831, 909, 1297, 1598
SsiI CCGC 3 cut(s) 330, 1549, 1624
StyD4I CCNGG 3 cut(s) 523, 982, 1153
StyI CCWWGG 2 cut(s) 648, 1441
TaaI ACNGT 7 cut(s) 146, 389, 566, 878, 1039, 1219, 1631
TaiI ACGT 5 cut(s) 432, 460, 909, 1322, 1619
TaqI TCGA 1 cut(s) 753
TaqII GACCGA 1 cut(s) 144
TasI AATT 7 cut(s) 501, 539, 722, 831, 909, 1297, 1598
TatI WGTACW 6 cut(s) 74, 317, 463, 612, 1068, 1368
TauI GCSGC 1 cut(s) 1551
TfiI GAWTC 1 cut(s) 1307
Tru1I TTAA 7 cut(s) 102, 110, 353, 1199, 1326, 1488, 1553
Tru9I TTAA 7 cut(s) 102, 110, 353, 1199, 1326, 1488, 1553
TscAI CASTG 5 cut(s) 187, 642, 744, 961, 1634
TseFI GTSAC 1 cut(s) 739
TseI GCWGC 5 cut(s) 218, 520, 1085, 1133, 1645
Tsp45I GTSAC 1 cut(s) 739
TspDTI ATGAA 6 cut(s) 30, 52, 273, 295, 691, 1446
TspGWI ACGGA 1 cut(s) 1235
TspRI CASTG 5 cut(s) 187, 642, 744, 961, 1634
Tth111I GACNNNGTC 1 cut(s) 188
Vha464I CTTAAG 1 cut(s) 1325
VneI GTGCAC 1 cut(s) 1632
VpaK11BI GGWCC 1 cut(s) 1463
XceI RCATGY 3 cut(s) 470, 1425, 1585
XcmI CCANNNNNNNNNTGG 1 cut(s) 1351
ZraI GACGTC 2 cut(s) 430, 458
ZrmI AGTACT 2 cut(s) 76, 319
Zsp2I ATGCAT 1 cut(s) 1583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.