Rroxscaffold_5G00356070

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000108
Physical Location & Seq
Forward (+)
35239351 .. 35242534
3184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00356070.1

Sequence Viewer

Length: 1605 bp
ATGGTGTCCTATGAGTATATTCTGGGTGGGTTCTTAGCTTCTTTGCTTGCCTTTGTTTTCTTCATGATCATCAGTACTACTCTTGGTGGAAACGAAAAGGTCAAAGCTTTAAGTGGAGGAGCAAAGAGTAATGGGTTTCAGCCGGAAATGGAGGAGGACAAAAGTACCGACGTCGTCATTGTCGGCGCAGGAGTTGCCGGTGCAGCTCTAGCTTACACTCTTGCCAAGGAAGGACGCCGTGTGCATGTCATCGAAAGAGACTTGACTGAGCCAGACAGAATTGTTGGTGAGCTGTTGCAGCCTGGGGGTTATCTCAAGTTGATTGAGTTGGGTCTTGAGGACTGTGCAAATGAGTCCATTGATGCTCAGAAGGTGTTTGGGTATGCTCTTTACAAAGATGGGAAGGATACAAAACTGTCTTACCCCTTGGAAAAGTACAGTGCAGATGTCGCCGGAAGAAGTTTCCACAATGGTCGTTTCATTCAAAGAATGCGAGAAAAAGCTGCAACTCTTGCAAATGTAAAATTGGAACAAGGATCAGTGACAACACTACTTGAGGAGAAGGGCATTGTCAAAGGTGTGATTTACAAGAACAAGGCTGGAGAGGAGCTGAGAGCATATGCTCCACTAACAATCGTGTGTGATGGATGCTTTTCAAATCTGCGCAAATCTCTCAGTGCTCCAAAGGTTGAAAATCCCTCTTGTTTTGTTGGTTTGATTTTGGAGAACTGTGACCTTCCACATGCAAATCATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTGTTTTATCCCATCAGTAACACCGAGGTTCGTTGTTTGGTAGATGTACCTGGAACAAAAGTGCCTTCAGTAGCTAATGGTGAAATGGCCAAGTATTTGAAATCTGTGGTGGCTCCTCAGATTCCCCCACAGCTGCACAATGCTTTTATGGCAGCAGTAGAGAAAGGAAACATCAGAACAATGCAAAACAAAAGCATGGCTGCTAATCCTGTTCCCACTCCTGGTGCAATTTTATTGGGGGATGCATTCAACATGAGGCATCCATTAACAGGAGGAGGAATGACTGTGGCTCTTTCAGACATTGTTCTTCTCCGTAATCTTCTTAGACCCCTACGTGATCTCAGTGACGCAACTGCTTTGTGCAATTACCTTGAAGCATTCTACACACTTCGTAAGTGTGCTTGGTTTTCATCGCAGCCAGTGTCATCTACCATAAACACATTGGCAGGTGCTTTATACAAGGTGTTTTGTGCATCACCTGATCCAGCAAAACAGGAAATGCGTGAAGCATGTTTTGACTATCTGAGCCTTGGAGGTATCTGTTCATATGGACCACTATCTCTGCTCTCTGGTCTTAACCCTCGTCCGCTGCACTTATTTCTCCATTTCTTTGCCGTTGCCATCTATGGAATCGGACGCTTAATGCTCCCCTTCCCTTCACCTCAGCGCATGTGGCTTGGGGCTAGATTGATCTTGGGATCTGATGTTGCTCTTGTTGACGGCAGCCATAAAAGCATTGTAGCTAGAGCTGAGGGGGAATCTGAAACCCTTGGACTAGCTAGTCCAGTTCAGGTAGACATTTGGATAAGTAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

534

Amino Acids

57.76

Weight (kDa)

7.11

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 56 - 371 1.2e-20 FAD binding domain
DAO PF01266 57 - 87 6.3e-08 FAD dependent oxidoreductase
NAD_binding_8 PF13450 60 - 86 2.4e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 208 - 487 6.3e-115 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1223
AasI GACNNNNNNGTC 1 cut(s) 1566
AatII GACGTC 1 cut(s) 174
Acc16I TGCGCA 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 1223
AccI GTMKAC 1 cut(s) 1581
AciI CCGC 1 cut(s) 1373
AclWI GGATC 3 cut(s) 544, 1262, 1492
AcoI YGGCCR 1 cut(s) 873
AcuI CTGAAG 1 cut(s) 837
AcyI GRCGYC 2 cut(s) 171, 235
AfaI GTAC 4 cut(s) 76, 166, 437, 834
AfiI CCNNNNNNNGG 2 cut(s) 1007, 1055
AflIII ACRYGT 1 cut(s) 757
AgsI TTSAA 6 cut(s) 485, 657, 692, 886, 1036, 1160
AjnI CCWGG 3 cut(s) 301, 835, 1006
Alw21I GWGCWC 1 cut(s) 682
Alw26I GTCTC 2 cut(s) 252, 765
AlwI GGATC 3 cut(s) 544, 1262, 1492
AoxI GGCC 1 cut(s) 873
ApeKI GCWGC 9 cut(s) 203, 298, 503, 919, 938, 986, 1201, 1375, 1509
AspLEI GCGC 3 cut(s) 188, 666, 1455
AspS9I GGNCC 1 cut(s) 1337
AsuHPI GGTGA 5 cut(s) 299, 771, 878, 1254, 1437
AvaII GGWCC 1 cut(s) 1337
BaeI ACNNNNGTAYC 2 cut(s) 148, 181
BalI TGGCCA 1 cut(s) 875
Bbv12I GWGCWC 1 cut(s) 682
BbvCI CCTCAGC 2 cut(s) 1449, 1536
BbvI GCAGC 9 cut(s) 215, 310, 490, 906, 950, 973, 1213, 1362, 1521
BccI CCATC 5 cut(s) 392, 638, 791, 806, 1415
BceAI ACGGC 3 cut(s) 222, 1385, 1522
BciT130I CCWGG 3 cut(s) 303, 837, 1008
BciVI GTATCC 1 cut(s) 400
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 252, 765
BfaI CTAG 5 cut(s) 209, 1470, 1530, 1562, 1566
BfuAI ACCTGC 1 cut(s) 1223
BfuI GTATCC 1 cut(s) 400
BisI GCNGC 9 cut(s) 204, 299, 504, 920, 939, 987, 1202, 1376, 1510
BlsI GCNGC 9 cut(s) 205, 300, 505, 921, 940, 988, 1203, 1377, 1511
BmcAI AGTACT 1 cut(s) 76
Bme1390I CCNGG 3 cut(s) 303, 837, 1008
Bme18I GGWCC 1 cut(s) 1337
BmgT120I GGNCC 1 cut(s) 1337
BmiI GGNNCC 1 cut(s) 900
BmrFI CCNGG 3 cut(s) 303, 837, 1008
BmsI GCATC 5 cut(s) 352, 638, 1018, 1054, 1268
BpmI CTGGAG 1 cut(s) 621
Bpu10I CCTNAGC 2 cut(s) 1449, 1536
BpuEI CTTGAG 3 cut(s) 299, 356, 575
BsaAI YACGTR 1 cut(s) 1121
BsaHI GRCGYC 2 cut(s) 171, 235
BsaI GGTCTC 1 cut(s) 765
BsaJI CCNNGG 6 cut(s) 225, 302, 426, 810, 1315, 1555
Bsc4I CCNNNNNNNGG 2 cut(s) 1007, 1055
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 2 cut(s) 1205, 1571
BseBI CCWGG 3 cut(s) 303, 837, 1008
BseDI CCNNGG 6 cut(s) 225, 302, 426, 810, 1315, 1555
BseGI GGATG 4 cut(s) 653, 783, 1033, 1045
BseLI CCNNNNNNNGG 2 cut(s) 1007, 1055
BseMII CTCAG 9 cut(s) 258, 380, 602, 688, 917, 1141, 1301, 1463, 1527
BseNI ACTGG 2 cut(s) 1205, 1571
BseRI GAGGAG 6 cut(s) 132, 167, 572, 620, 891, 1074
BseXI GCAGC 9 cut(s) 215, 310, 490, 906, 950, 973, 1213, 1362, 1521
BsgI GTGCAG 4 cut(s) 222, 462, 905, 1361
BshFI GGCC 1 cut(s) 875
BsiHKAI GWGCWC 1 cut(s) 682
BsiSI CCGG 3 cut(s) 143, 198, 453
BslI CCNNNNNNNGG 2 cut(s) 1007, 1055
BsmAI GTCTC 2 cut(s) 252, 765
BsmI GAATGC 3 cut(s) 495, 1031, 1163
BsnI GGCC 1 cut(s) 875
Bso31I GGTCTC 1 cut(s) 765
Bsp1286I GDGCHC 1 cut(s) 682
Bsp143I GATC 6 cut(s) 66, 536, 1123, 1267, 1476, 1484
BspACI CCGC 1 cut(s) 1373
BspANI GGCC 1 cut(s) 875
BspCNI CTCAG 9 cut(s) 259, 379, 603, 687, 916, 1140, 1302, 1462, 1528
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 900
BspMI ACCTGC 1 cut(s) 1223
BspPI GGATC 3 cut(s) 544, 1262, 1492
BspTNI GGTCTC 1 cut(s) 765
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 2 cut(s) 1205, 1571
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 6 cut(s) 225, 302, 426, 810, 1315, 1555
BssMI GATC 6 cut(s) 66, 536, 1123, 1267, 1476, 1484
BssNI GRCGYC 2 cut(s) 171, 235
BssT1I CCWWGG 4 cut(s) 225, 426, 1315, 1555
Bst2UI CCWGG 3 cut(s) 303, 837, 1008
Bst4CI ACNGT 5 cut(s) 344, 417, 440, 731, 1072
BstACI GRCGYC 2 cut(s) 171, 235
BstAPI GCANNNNNTGC 2 cut(s) 194, 512
BstBAI YACGTR 1 cut(s) 1121
BstC8I GCNNGC 1 cut(s) 48
BstF5I GGATG 4 cut(s) 653, 783, 1033, 1045
BstHHI GCGC 3 cut(s) 188, 666, 1455
BstKTI GATC 6 cut(s) 69, 539, 1126, 1270, 1479, 1487
BstMAI GTCTC 2 cut(s) 252, 765
BstMBI GATC 6 cut(s) 66, 536, 1123, 1267, 1476, 1484
BstMWI GCNNNNNNNGC 9 cut(s) 194, 203, 209, 298, 449, 512, 935, 1459, 1518
BstNI CCWGG 3 cut(s) 303, 837, 1008
BstNSI RCATGY 5 cut(s) 248, 746, 761, 1299, 1459
BstSCI CCNGG 3 cut(s) 301, 835, 1006
BstV1I GCAGC 9 cut(s) 215, 310, 490, 906, 950, 973, 1213, 1362, 1521
BstX2I RGATCY 1 cut(s) 1484
BstYI RGATCY 1 cut(s) 1484
BsuI GTATCC 1 cut(s) 400
BsuRI GGCC 1 cut(s) 875
BtgZI GCGATG 1 cut(s) 1182
BtsCI GGATG 4 cut(s) 653, 783, 1033, 1045
BtsIMutI CAGTG 5 cut(s) 445, 546, 682, 1135, 1212
BveI ACCTGC 1 cut(s) 1223
Cac8I GCNNGC 1 cut(s) 48
CciI TCATGA 1 cut(s) 63
CfoI GCGC 3 cut(s) 188, 666, 1455
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 1337
CseI GACGC 3 cut(s) 243, 1142, 1431
Csp6I GTAC 4 cut(s) 75, 165, 436, 833
CviAII CATG 9 cut(s) 64, 245, 743, 752, 758, 982, 1039, 1296, 1456
CviQI GTAC 4 cut(s) 75, 165, 436, 833
DpnI GATC 6 cut(s) 68, 538, 1125, 1269, 1478, 1486
DpnII GATC 6 cut(s) 66, 536, 1123, 1267, 1476, 1484
DrdI GACNNNNNNGTC 1 cut(s) 1566
DseDI GACNNNNNNGTC 1 cut(s) 1566
EaeI YGGCCR 1 cut(s) 873
Eco130I CCWWGG 4 cut(s) 225, 426, 1315, 1555
Eco31I GGTCTC 1 cut(s) 765
Eco47I GGWCC 1 cut(s) 1337
Eco57I CTGAAG 1 cut(s) 837
EcoRII CCWGG 3 cut(s) 301, 835, 1006
EcoT14I CCWWGG 4 cut(s) 225, 426, 1315, 1555
EcoT22I ATGCAT 1 cut(s) 1033
ErhI CCWWGG 4 cut(s) 225, 426, 1315, 1555
FaeI CATG 9 cut(s) 67, 248, 746, 755, 761, 985, 1042, 1299, 1459
FatI CATG 9 cut(s) 63, 244, 742, 751, 757, 981, 1038, 1295, 1455
FauNDI CATATG 2 cut(s) 619, 1333
FbaI TGATCA 1 cut(s) 66
FblI GTMKAC 1 cut(s) 1581
Fnu4HI GCNGC 9 cut(s) 204, 299, 504, 920, 939, 987, 1202, 1376, 1510
FokI GGATG 4 cut(s) 660, 770, 1032, 1040
Fsp4HI GCNGC 9 cut(s) 204, 299, 504, 920, 939, 987, 1202, 1376, 1510
FspBI CTAG 5 cut(s) 209, 1470, 1530, 1562, 1566
FspI TGCGCA 1 cut(s) 665
GlaI GCGC 3 cut(s) 187, 665, 1454
GluI GCNGC 9 cut(s) 204, 299, 504, 920, 939, 987, 1202, 1376, 1510
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 1 cut(s) 875
HapII CCGG 3 cut(s) 143, 198, 453
HgaI GACGC 3 cut(s) 243, 1142, 1431
HhaI GCGC 3 cut(s) 188, 666, 1455
Hin1I GRCGYC 2 cut(s) 171, 235
Hin1II CATG 9 cut(s) 67, 248, 746, 755, 761, 985, 1042, 1299, 1459
Hin6I GCGC 3 cut(s) 186, 664, 1453
HinP1I GCGC 3 cut(s) 186, 664, 1453
HincII GTYRAC 1 cut(s) 1504
HindII GTYRAC 1 cut(s) 1504
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 4 cut(s) 353, 907, 1416, 1544
HpaII CCGG 3 cut(s) 143, 198, 453
HphI GGTGA 5 cut(s) 299, 771, 878, 1254, 1437
Hpy166II GTNNAC 2 cut(s) 1504, 1582
Hpy188I TCNGA 8 cut(s) 369, 906, 962, 1084, 1311, 1421, 1489, 1549
Hpy188III TCNNGA 2 cut(s) 64, 335
Hpy8I GTNNAC 2 cut(s) 1504, 1582
Hpy99I CGWCG 2 cut(s) 173, 176
HpyAV CCTTC 9 cut(s) 224, 364, 397, 556, 746, 786, 861, 1447, 1452
HpyCH4III ACNGT 5 cut(s) 344, 417, 440, 731, 1072
HpyCH4IV ACGT 2 cut(s) 171, 1120
HpyF10VI GCNNNNNNNGC 9 cut(s) 194, 203, 209, 298, 449, 512, 935, 1459, 1518
HpySE526I ACGT 2 cut(s) 171, 1120
Hsp92I GRCGYC 2 cut(s) 171, 235
Hsp92II CATG 9 cut(s) 67, 248, 746, 755, 761, 985, 1042, 1299, 1459
HspAI GCGC 3 cut(s) 186, 664, 1453
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 6 cut(s) 66, 536, 1123, 1267, 1476, 1484
LmnI GCTCC 6 cut(s) 119, 607, 628, 685, 904, 1437
Lsp1109I GCAGC 9 cut(s) 215, 310, 490, 906, 950, 973, 1213, 1362, 1521
LweI GCATC 5 cut(s) 352, 638, 1018, 1054, 1268
MaeI CTAG 5 cut(s) 209, 1470, 1530, 1562, 1566
MaeII ACGT 2 cut(s) 171, 1120
MaeIII GTNAC 4 cut(s) 541, 731, 803, 1130
MalI GATC 6 cut(s) 68, 538, 1125, 1269, 1478, 1486
MboI GATC 6 cut(s) 66, 536, 1123, 1267, 1476, 1484
MboII GAAGA 4 cut(s) 52, 468, 1085, 1097
MflI RGATCY 1 cut(s) 1484
MhlI GDGCHC 1 cut(s) 682
MlsI TGGCCA 1 cut(s) 875
MluCI AATT 5 cut(s) 279, 524, 1014, 1150, 1600
MluNI TGGCCA 1 cut(s) 875
MlyI GAGTC 1 cut(s) 362
Mox20I TGGCCA 1 cut(s) 875
Mph1103I ATGCAT 1 cut(s) 1033
MscI TGGCCA 1 cut(s) 875
MseI TTAA 5 cut(s) 110, 1052, 1362, 1427, 1603
MslI CAYNNNNRTG 1 cut(s) 756
Msp20I TGGCCA 1 cut(s) 875
MspA1I CMGCKG 2 cut(s) 919, 1375
MspI CCGG 3 cut(s) 143, 198, 453
MspR9I CCNGG 3 cut(s) 303, 837, 1008
Mva1269I GAATGC 3 cut(s) 495, 1031, 1163
MvaI CCWGG 3 cut(s) 303, 837, 1008
MwoI GCNNNNNNNGC 9 cut(s) 194, 203, 209, 298, 449, 512, 935, 1459, 1518
NdeI CATATG 2 cut(s) 619, 1333
NdeII GATC 6 cut(s) 66, 536, 1123, 1267, 1476, 1484
NlaIII CATG 9 cut(s) 67, 248, 746, 755, 761, 985, 1042, 1299, 1459
NlaIV GGNNCC 1 cut(s) 900
NmuCI GTSAC 3 cut(s) 541, 731, 1130
NsbI TGCGCA 1 cut(s) 665
NsiI ATGCAT 1 cut(s) 1033
NspI RCATGY 5 cut(s) 248, 746, 761, 1299, 1459
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 1 cut(s) 1223
PciI ACATGT 1 cut(s) 757
PctI GAATGC 3 cut(s) 495, 1031, 1163
PfeI GAWTC 3 cut(s) 907, 1416, 1544
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 9 cut(s) 205, 300, 505, 921, 940, 988, 1203, 1377, 1511
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
Ppu21I YACGTR 1 cut(s) 1121
PscI ACATGT 1 cut(s) 757
Psp6I CCWGG 3 cut(s) 301, 835, 1006
PspGI CCWGG 3 cut(s) 301, 835, 1006
PspN4I GGNNCC 1 cut(s) 900
PspPI GGNCC 1 cut(s) 1337
PsuI RGATCY 1 cut(s) 1484
PsyI GACNNNGTC 1 cut(s) 173
PvuII CAGCTG 1 cut(s) 919
RsaI GTAC 4 cut(s) 76, 166, 437, 834
RsaNI GTAC 4 cut(s) 75, 165, 436, 833
RseI CAYNNNNRTG 1 cut(s) 756
SaqAI TTAA 5 cut(s) 110, 1052, 1362, 1427, 1603
SatI GCNGC 9 cut(s) 204, 299, 504, 920, 939, 987, 1202, 1376, 1510
Sau3AI GATC 6 cut(s) 66, 536, 1123, 1267, 1476, 1484
Sau96I GGNCC 1 cut(s) 1337
ScaI AGTACT 1 cut(s) 76
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 3 cut(s) 303, 837, 1008
SduI GDGCHC 1 cut(s) 682
SfaNI GCATC 5 cut(s) 352, 638, 1018, 1054, 1268
SinI GGWCC 1 cut(s) 1337
SmiMI CAYNNNNRTG 1 cut(s) 756
SmlI CTYRAG 3 cut(s) 314, 335, 554
SmoI CTYRAG 3 cut(s) 314, 335, 554
Sse9I AATT 5 cut(s) 279, 524, 1014, 1150, 1600
SsiI CCGC 1 cut(s) 1373
SspMI CTAG 5 cut(s) 209, 1470, 1530, 1562, 1566
StyD4I CCNGG 3 cut(s) 301, 835, 1006
StyI CCWWGG 4 cut(s) 225, 426, 1315, 1555
TaaI ACNGT 5 cut(s) 344, 417, 440, 731, 1072
TaiI ACGT 2 cut(s) 174, 1123
TaqI TCGA 1 cut(s) 252
TasI AATT 5 cut(s) 279, 524, 1014, 1150, 1600
TatI WGTACW 2 cut(s) 74, 435
TfiI GAWTC 3 cut(s) 907, 1416, 1544
Tru1I TTAA 5 cut(s) 110, 1052, 1362, 1427, 1603
Tru9I TTAA 5 cut(s) 110, 1052, 1362, 1427, 1603
TscAI CASTG 5 cut(s) 445, 546, 682, 1135, 1212
TseFI GTSAC 3 cut(s) 541, 731, 1130
TseI GCWGC 9 cut(s) 203, 298, 503, 919, 938, 986, 1201, 1375, 1509
Tsp45I GTSAC 3 cut(s) 541, 731, 1130
TspDTI ATGAA 4 cut(s) 52, 469, 1185, 1320
TspGWI ACGGA 1 cut(s) 1088
TspRI CASTG 5 cut(s) 445, 546, 682, 1135, 1212
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 1337
XceI RCATGY 5 cut(s) 248, 746, 761, 1299, 1459
XcmI CCANNNNNNNNNTGG 1 cut(s) 1225
XmiI GTMKAC 1 cut(s) 1581
XspI CTAG 5 cut(s) 209, 1470, 1530, 1562, 1566
ZraI GACGTC 1 cut(s) 172
ZrmI AGTACT 1 cut(s) 76
Zsp2I ATGCAT 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.