Rmu_co8422277.1_g000001

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8422277.1
OG0000108
Physical Location & Seq
Forward (+)
1 .. 1421
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8422277.1_g000001.1.cds

Sequence Viewer

Length: 968 bp
tcgtcgtgtacatgtcatcgaaagagacttgactgagccagacagaattgttggtgagctgttgcagcctgggggttatcttaagttgattgagttgggtctcgacgagtgtgcaaatgagtcaattgatgctcagaaggtgtttggttatgctctttataaagatggcaaggataccaaactgacttatcccttggagaaatacagttcagatgtggctggaagaagtttccacaatgggcgtttcatccaaagaatgcgtgaaaaagctatgactctttcaaatgtaaaattggaacaaggatcagtgacaacactaatcgaggaaaagggcattgtcaaaggggtgatttacaagaacaaggctggagaggagatgagaacatatgctcctctaacaatcgtgtgtgatggttgcttttcaaatctgcgcaagtctctcagtgcaccaaagattgaaagtccctcttgttttgttggtttaatcttggaaaactgtgaccttccacacgcaaatcatggacatgtgattctgggagacccttcacccatcctcttttatcctatcagtagcaccgaggttcgttgtttggtggatgtacctggaacaaaagtaccttcagtagctagtggtgaaatggcaaactacttgaaaaccgtggtggctcctcagattcccccacagctgcacaatgcttttatggccgcagtagagaagggaaacatcagaacaatgcaaaacaaaagcatggctgctaatcctatccctactcctggtgcaattttattaggggatgcattcaacatgaggcatcctttaacaggaggaggaatgactgttgctctttctgacattgttcttctccgcgatcttcttagaccgctgtgtgatctcaatgatgcacctgctttgtgcaattaccttgaatcattctacacactgcgcaaggtaactaaaatatcttctt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

322

Amino Acids

35.32

Weight (kDa)

7.7

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 160
AarI CACCTGC 1 cut(s) 914
Acc16I TGCGCA 2 cut(s) 432, 945
Acc36I ACCTGC 1 cut(s) 914
AccII CGCG 1 cut(s) 868
AciI CCGC 3 cut(s) 706, 866, 882
AclWI GGATC 1 cut(s) 311
AcoI YGGCCR 1 cut(s) 703
AcuI CTGAAG 1 cut(s) 604
AfaI GTAC 3 cut(s) 10, 601, 616
AfiI CCNNNNNNNGG 2 cut(s) 774, 822
AflII CTTAAG 1 cut(s) 81
AflIII ACRYGT 2 cut(s) 11, 524
AgsI TTSAA 6 cut(s) 283, 424, 459, 653, 803, 927
AjnI CCWGG 3 cut(s) 68, 602, 773
AloI GAACNNNNNNTCC 2 cut(s) 374, 406
AluBI AGCT 4 cut(s) 59, 270, 627, 686
AluI AGCT 4 cut(s) 59, 270, 627, 686
Alw21I GWGCWC 1 cut(s) 449
Alw26I GTCTC 4 cut(s) 19, 105, 442, 532
Alw44I GTGCAC 1 cut(s) 445
AlwI GGATC 1 cut(s) 311
AoxI GGCC 1 cut(s) 703
ApaLI GTGCAC 1 cut(s) 445
ApeKI GCWGC 3 cut(s) 65, 686, 753
AspLEI GCGC 2 cut(s) 433, 946
AsuHPI GGTGA 4 cut(s) 66, 359, 538, 645
BaeGI GKGCMC 1 cut(s) 449
BaeI ACNNNNGTAYC 2 cut(s) 598, 631
Bbv12I GWGCWC 1 cut(s) 449
BbvI GCAGC 3 cut(s) 77, 673, 740
BccI CCATC 3 cut(s) 159, 405, 558
BcgI CGANNNNNNTGC 2 cut(s) 93, 127
BciT130I CCWGG 3 cut(s) 70, 604, 775
BciVI GTATCC 1 cut(s) 167
BcoDI GTCTC 4 cut(s) 19, 105, 442, 532
BfaI CTAG 1 cut(s) 628
BfrI CTTAAG 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 914
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 4 cut(s) 66, 687, 706, 754
BlsI GCNGC 4 cut(s) 67, 688, 707, 755
Bme1390I CCNGG 3 cut(s) 70, 604, 775
BmiI GGNNCC 1 cut(s) 667
BmrFI CCNGG 3 cut(s) 70, 604, 775
BmsI GCATC 4 cut(s) 119, 785, 821, 890
BpmI CTGGAG 1 cut(s) 388
BsaI GGTCTC 2 cut(s) 105, 532
BsaJI CCNNGG 4 cut(s) 69, 193, 577, 658
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 774, 822
BseBI CCWGG 3 cut(s) 70, 604, 775
BseDI CCNNGG 4 cut(s) 69, 193, 577, 658
BseGI GGATG 5 cut(s) 247, 550, 602, 800, 812
BseLI CCNNNNNNNGG 2 cut(s) 774, 822
BseMII CTCAG 4 cut(s) 25, 147, 455, 684
BseRI GAGGAG 4 cut(s) 382, 387, 658, 841
BseSI GKGCMC 1 cut(s) 449
BseXI GCAGC 3 cut(s) 77, 673, 740
BsgI GTGCAG 1 cut(s) 672
Bsh1236I CGCG 1 cut(s) 868
BshFI GGCC 1 cut(s) 705
BsiHKAI GWGCWC 1 cut(s) 449
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 2 cut(s) 774, 822
BsmAI GTCTC 4 cut(s) 19, 105, 442, 532
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 2 cut(s) 262, 798
BsnI GGCC 1 cut(s) 705
Bso31I GGTCTC 2 cut(s) 105, 532
Bsp1286I GDGCHC 1 cut(s) 449
Bsp1407I TGTACA 1 cut(s) 8
Bsp143I GATC 3 cut(s) 303, 869, 890
BspACI CCGC 3 cut(s) 706, 866, 882
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 4 cut(s) 26, 146, 454, 683
BspFNI CGCG 1 cut(s) 868
BspLI GGNNCC 1 cut(s) 667
BspMI ACCTGC 1 cut(s) 914
BspPI GGATC 1 cut(s) 311
BspTI CTTAAG 1 cut(s) 81
BspTNI GGTCTC 2 cut(s) 105, 532
BsrGI TGTACA 1 cut(s) 8
BssECI CCNNGG 4 cut(s) 69, 193, 577, 658
BssMI GATC 3 cut(s) 303, 869, 890
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 3 cut(s) 70, 604, 775
Bst4CI ACNGT 4 cut(s) 207, 498, 659, 839
BstAFI CTTAAG 1 cut(s) 81
BstAUI TGTACA 1 cut(s) 8
BstDEI CTNAG 5 cut(s) 34, 133, 441, 670, 876
BstDSI CCRYGG 1 cut(s) 658
BstENI CCTNNNNNAGG 1 cut(s) 820
BstF5I GGATG 5 cut(s) 247, 550, 602, 800, 812
BstFNI CGCG 1 cut(s) 868
BstHHI GCGC 2 cut(s) 433, 946
BstKTI GATC 3 cut(s) 306, 872, 893
BstMAI GTCTC 4 cut(s) 19, 105, 442, 532
BstMBI GATC 3 cut(s) 303, 869, 890
BstMWI GCNNNNNNNGC 2 cut(s) 65, 702
BstNI CCWGG 3 cut(s) 70, 604, 775
BstNSI RCATGY 2 cut(s) 15, 528
BstSCI CCNGG 3 cut(s) 68, 602, 773
BstSLI GKGCMC 1 cut(s) 449
BstUI CGCG 1 cut(s) 868
BstV1I GCAGC 3 cut(s) 77, 673, 740
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 658
BtsCI GGATG 5 cut(s) 247, 550, 602, 800, 812
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 3 cut(s) 313, 449, 939
BveI ACCTGC 1 cut(s) 914
CfoI GCGC 2 cut(s) 433, 946
Csp6I GTAC 3 cut(s) 9, 600, 615
CviAII CATG 5 cut(s) 12, 519, 525, 749, 806
CviQI GTAC 3 cut(s) 9, 600, 615
DdeI CTNAG 5 cut(s) 34, 133, 441, 670, 876
DpnI GATC 3 cut(s) 305, 871, 892
DpnII GATC 3 cut(s) 303, 869, 890
EaeI YGGCCR 1 cut(s) 703
Eco130I CCWWGG 1 cut(s) 193
Eco31I GGTCTC 2 cut(s) 105, 532
Eco57I CTGAAG 1 cut(s) 604
EcoNI CCTNNNNNAGG 1 cut(s) 820
EcoRII CCWGG 3 cut(s) 68, 602, 773
EcoT14I CCWWGG 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 800
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 5 cut(s) 15, 522, 528, 752, 809
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FaqI GGGAC 1 cut(s) 448
FatI CATG 5 cut(s) 11, 518, 524, 748, 805
FauNDI CATATG 1 cut(s) 386
Fnu4HI GCNGC 4 cut(s) 66, 687, 706, 754
FokI GGATG 5 cut(s) 234, 537, 609, 799, 807
Fsp4HI GCNGC 4 cut(s) 66, 687, 706, 754
FspBI CTAG 1 cut(s) 628
FspI TGCGCA 2 cut(s) 432, 945
GlaI GCGC 2 cut(s) 432, 945
GluI GCNGC 4 cut(s) 66, 687, 706, 754
GsuI CTGGAG 1 cut(s) 388
HaeIII GGCC 1 cut(s) 705
HhaI GCGC 2 cut(s) 433, 946
Hin1II CATG 5 cut(s) 15, 522, 528, 752, 809
Hin6I GCGC 2 cut(s) 431, 944
HinP1I GCGC 2 cut(s) 431, 944
HinfI GANTC 5 cut(s) 120, 275, 530, 674, 927
HphI GGTGA 4 cut(s) 66, 359, 538, 645
Hpy166II GTNNAC 2 cut(s) 9, 447
Hpy188I TCNGA 5 cut(s) 136, 212, 673, 729, 851
Hpy188III TCNNGA 1 cut(s) 102
Hpy8I GTNNAC 2 cut(s) 9, 447
Hpy99I CGWCG 2 cut(s) 7, 108
HpyAV CCTTC 5 cut(s) 131, 513, 553, 628, 710
HpyCH4III ACNGT 4 cut(s) 207, 498, 659, 839
HpyCH4V TGCA 9 cut(s) 65, 114, 447, 689, 737, 780, 798, 903, 916
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 702
HpyF3I CTNAG 5 cut(s) 34, 133, 441, 670, 876
Hsp92II CATG 5 cut(s) 15, 522, 528, 752, 809
HspAI GCGC 2 cut(s) 431, 944
Kzo9I GATC 3 cut(s) 303, 869, 890
LmnI GCTCC 2 cut(s) 395, 671
Lsp1109I GCAGC 3 cut(s) 77, 673, 740
LweI GCATC 4 cut(s) 119, 785, 821, 890
MaeI CTAG 1 cut(s) 628
MaeIII GTNAC 3 cut(s) 308, 498, 950
MalI GATC 3 cut(s) 305, 871, 892
MboI GATC 3 cut(s) 303, 869, 890
MboII GAAGA 4 cut(s) 235, 852, 864, 955
MfeI CAATTG 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 449
MluCI AATT 5 cut(s) 46, 124, 291, 781, 917
MlyI GAGTC 2 cut(s) 129, 269
Mph1103I ATGCAT 1 cut(s) 800
MseI TTAA 3 cut(s) 82, 483, 819
MslI CAYNNNNRTG 1 cut(s) 523
MspA1I CMGCKG 2 cut(s) 686, 884
MspCI CTTAAG 1 cut(s) 81
MspR9I CCNGG 3 cut(s) 70, 604, 775
MunI CAATTG 1 cut(s) 124
Mva1269I GAATGC 2 cut(s) 262, 798
MvaI CCWGG 3 cut(s) 70, 604, 775
MvnI CGCG 1 cut(s) 868
MwoI GCNNNNNNNGC 2 cut(s) 65, 702
NdeI CATATG 1 cut(s) 386
NdeII GATC 3 cut(s) 303, 869, 890
NlaIII CATG 5 cut(s) 15, 522, 528, 752, 809
NlaIV GGNNCC 1 cut(s) 667
NmuCI GTSAC 2 cut(s) 308, 498
NsbI TGCGCA 2 cut(s) 432, 945
NsiI ATGCAT 1 cut(s) 800
NspI RCATGY 2 cut(s) 15, 528
PaqCI CACCTGC 1 cut(s) 914
PciI ACATGT 2 cut(s) 11, 524
PctI GAATGC 2 cut(s) 262, 798
PfeI GAWTC 3 cut(s) 530, 674, 927
PkrI GCNGC 4 cut(s) 67, 688, 707, 755
PleI GAGTC 2 cut(s) 128, 269
PpsI GAGTC 2 cut(s) 128, 269
PscI ACATGT 2 cut(s) 11, 524
PsiI TTATAA 1 cut(s) 160
Psp6I CCWGG 3 cut(s) 68, 602, 773
PspGI CCWGG 3 cut(s) 68, 602, 773
PspN4I GGNNCC 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 3 cut(s) 10, 601, 616
RsaNI GTAC 3 cut(s) 9, 600, 615
RseI CAYNNNNRTG 1 cut(s) 523
SaqAI TTAA 3 cut(s) 82, 483, 819
SatI GCNGC 4 cut(s) 66, 687, 706, 754
Sau3AI GATC 3 cut(s) 303, 869, 890
SchI GAGTC 2 cut(s) 129, 269
ScrFI CCNGG 3 cut(s) 70, 604, 775
SduI GDGCHC 1 cut(s) 449
SfaNI GCATC 4 cut(s) 119, 785, 821, 890
SmiMI CAYNNNNRTG 1 cut(s) 523
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
Sse9I AATT 5 cut(s) 46, 124, 291, 781, 917
SsiI CCGC 3 cut(s) 706, 866, 882
SspMI CTAG 1 cut(s) 628
StyD4I CCNGG 3 cut(s) 68, 602, 773
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 4 cut(s) 207, 498, 659, 839
TaqI TCGA 3 cut(s) 19, 103, 322
TasI AATT 5 cut(s) 46, 124, 291, 781, 917
TatI WGTACW 1 cut(s) 8
TauI GCSGC 1 cut(s) 708
TfiI GAWTC 3 cut(s) 530, 674, 927
Tru1I TTAA 3 cut(s) 82, 483, 819
Tru9I TTAA 3 cut(s) 82, 483, 819
TscAI CASTG 3 cut(s) 313, 449, 946
TseFI GTSAC 2 cut(s) 308, 498
TseI GCWGC 3 cut(s) 65, 686, 753
Tsp45I GTSAC 2 cut(s) 308, 498
TspDTI ATGAA 1 cut(s) 236
TspRI CASTG 3 cut(s) 313, 449, 946
Vha464I CTTAAG 1 cut(s) 81
VneI GTGCAC 1 cut(s) 445
XagI CCTNNNNNAGG 1 cut(s) 820
XceI RCATGY 2 cut(s) 15, 528
XspI CTAG 1 cut(s) 628
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.