RchiOBHm_Chr6g0246991

Squalene monooxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000108
Physical Location & Seq
Forward (+)
2763125 .. 2766591
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22140

Sequence Viewer

Length: 1587 bp
ATGGTTTCTTACGAGTACGTTCTGGGTGGAGTCCTGGCTTCTCTGCTGGCCTCTGTTTTCATCATGATAATCAATAGTAGTAGAAAGGTGAAGGTAGTGGGGGGTGCAAGGAGTGTTAATGGGTTTGTGAGGTTGCCGCAAAACGGTGCGTTTGCGCCGGCGGTGGTGGTGGAGGAGGAGAAAGGTACCGACGTCGTCATTGTCGGAGCCGGAGTTGCCGGTGCAGCTCTTGCTTACACTCTTGCCAAGGAAGGACGCCGTGTACATGTCATCGAAAGAGACTTGAGTGAGCCGGACAGAATTGTTGGCGAGCTGTTGCAGCCTGGGGGTTATCTCAAGTTGATCGAGTTGGGTCTCGAGGACTGTGCAAATGAATCCATTGATGCTCAGAAGGTTTTTGGTTATGCTCTCTACAAAGATGGGAAGGATACAAAACTTTCTTATCCCTTGGAAAATTACAGTTCAGATATAGCTGGGAGAAGTTTCCACAATGGGCGTTTCATCCAAAGAATGCGTGAAAAAGCTGCAACTCTTTCAAATGTAAAATTGGAACAAGGGACAGTGACAACACTAATTGAAGAAAAGGGCACTATCAAAGGAGTGATTTACAAGAACAAGGCTGGAGAGGAGTTCAGAACATATGCTCCACTAACAATCGTGTGTGATGGCTGCTTTTCAAATCTGCGCAAAAATCTCAGTGCTCCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGGTGTTGGAGAACTGTGACCTGCCACACGCAAATCATGGTCATGTGATTTTGGGAGACCCTTCACCCGTCCTGTTTTATCCTATCAGTAGCACAGAGATTCGTTGTTTGGTTGATGTACCTGGAACAAAAGTTCCTTCAGTAGCTAGTGGTGAAATGGCTAAGTATTTGAAAACCGTGGTGGCTCCTCAGATTCCTCCTCAGCTCTACAATTCTTTTATAGCTGCAATTGACAAGGGAAACATCAAATCCATGCAAAACAAAAGCATGGCTGCAAATCCTCTTCCCACTCCTGGTGCACTTTTATTGGGGGATTCATTCAACATGAGGCATCCTTTGACAGGAGGAGGAATGACTGTGGCTCTTGCAGACATCGTTCTTCTTCGGGATCTTCTTAAACCCCTACATGATCTCAATGATGCAGCTGCTTTGTGCAATTACCTTGAATCATTCTACACATTGCGTAAGCCTGTGTCATCTACCATAAACACATTGGCAGGTGCCCTGTACAAGGTGTTTTGTGCATCACCTGATCCAGCAAGACAGGAAATGCGTGAAGCATGTTTTGGTTATTTGAGCCTTGGAGGCATCTGTTCATATGGACCAGTATCTCTTCTCTCTGGTCTTAACCCTCGTCCACTTCACCTATTTCTCCATTTCTTTGCTGTTGCTATCTATGGTGTTGGGCGCTTGATGCTTCCATTCCCTTCTCCTCAACGCACATGGCTTGGGGTTAGATTGATCTTGAGTGCATCAGGGATCATATTTCCCATTATAAAGGGTGAAGGAGTTAGACAGATGTTCTTTCCTGCAACAGTACCAGCATATTACAGAGCTCCTCCTGTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

528

Amino Acids

57.07

Weight (kDa)

8.74

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 63 - 375 1.2e-19 FAD binding domain
DAO PF01266 64 - 94 4.9e-08 FAD dependent oxidoreductase
FAD_binding_2 PF00890 64 - 93 1e-05 FAD binding domain
NAD_binding_8 PF13450 67 - 93 2.1e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 215 - 483 1.8e-117 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1511
AarI CACCTGC 1 cut(s) 1223
AatII GACGTC 1 cut(s) 195
Acc16I TGCGCA 1 cut(s) 686
Acc36I ACCTGC 2 cut(s) 765, 1223
Acc65I GGTACC 1 cut(s) 185
AccB1I GGYRCC 2 cut(s) 185, 1235
AciI CCGC 2 cut(s) 137, 161
AclWI GGATC 3 cut(s) 1131, 1262, 1502
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 2 cut(s) 192, 256
AfaI GTAC 6 cut(s) 17, 187, 264, 855, 1244, 1554
AfiI CCNNNNNNNGG 4 cut(s) 143, 1028, 1076, 1246
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 8 cut(s) 537, 578, 678, 713, 907, 1057, 1181, 1583
AjnI CCWGG 4 cut(s) 33, 322, 856, 1027
AjuI GAANNNNNNNTTGG 2 cut(s) 1284, 1316
AloI GAACNNNNNNTCC 6 cut(s) 628, 660, 852, 853, 884, 885
AluBI AGCT 9 cut(s) 227, 313, 473, 524, 881, 940, 959, 1160, 1571
AluI AGCT 9 cut(s) 227, 313, 473, 524, 881, 940, 959, 1160, 1571
Alw21I GWGCWC 3 cut(s) 703, 1036, 1573
Alw26I GTCTC 3 cut(s) 273, 359, 786
Alw44I GTGCAC 1 cut(s) 1032
AlwI GGATC 3 cut(s) 1131, 1262, 1502
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 1 cut(s) 48
ApaLI GTGCAC 1 cut(s) 1032
ApeKI GCWGC 8 cut(s) 224, 319, 524, 669, 959, 1007, 1157, 1160
Asp718I GGTACC 1 cut(s) 185
AspLEI GCGC 3 cut(s) 157, 687, 1425
AspS9I GGNCC 1 cut(s) 1337
AsuHPI GGTGA 6 cut(s) 100, 792, 899, 1254, 1370, 1529
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 1 cut(s) 1337
BaeGI GKGCMC 3 cut(s) 590, 1036, 1240
BaeI ACNNNNGTAYC 2 cut(s) 177, 210
BanI GGYRCC 2 cut(s) 185, 1235
BanII GRGCYC 1 cut(s) 1573
Bbv12I GWGCWC 3 cut(s) 703, 1036, 1573
BbvCI CCTCAGC 1 cut(s) 936
BbvI GCAGC 8 cut(s) 236, 331, 511, 656, 946, 994, 1147, 1169
BccI CCATC 2 cut(s) 413, 659
BceAI ACGGC 1 cut(s) 243
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciT130I CCWGG 4 cut(s) 35, 324, 858, 1029
BciVI GTATCC 1 cut(s) 421
BcoDI GTCTC 3 cut(s) 273, 359, 786
BfaI CTAG 1 cut(s) 882
BfoI RGCGCY 1 cut(s) 1426
BfuAI ACCTGC 2 cut(s) 765, 1223
BfuI GTATCC 1 cut(s) 421
BglI GCCNNNNNGGC 1 cut(s) 1320
BisI GCNGC 9 cut(s) 137, 225, 320, 525, 670, 960, 1008, 1158, 1161
BlsI GCNGC 9 cut(s) 138, 226, 321, 526, 671, 961, 1009, 1159, 1162
Bme1390I CCNGG 4 cut(s) 35, 324, 858, 1029
Bme18I GGWCC 1 cut(s) 1337
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 1337
BmiI GGNNCC 4 cut(s) 187, 208, 921, 1237
BmrFI CCNGG 4 cut(s) 35, 324, 858, 1029
BmsI GCATC 7 cut(s) 373, 1075, 1144, 1268, 1332, 1419, 1496
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 936
BpuEI CTTGAG 3 cut(s) 304, 320, 1501
BsaHI GRCGYC 2 cut(s) 192, 256
BsaI GGTCTC 2 cut(s) 359, 786
BsaJI CCNNGG 5 cut(s) 246, 323, 447, 912, 1315
BsaXI ACNNNNNCTCC 4 cut(s) 198, 228, 628, 658
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 1028, 1076, 1246
Bse118I RCCGGY 2 cut(s) 157, 218
Bse1I ACTGG 1 cut(s) 1340
Bse3DI GCAATG 1 cut(s) 1193
BseBI CCWGG 4 cut(s) 35, 324, 858, 1029
BseDI CCNNGG 5 cut(s) 246, 323, 447, 912, 1315
BseGI GGATG 2 cut(s) 501, 1066
BseLI CCNNNNNNNGG 4 cut(s) 143, 1028, 1076, 1246
BseMI GCAATG 1 cut(s) 1193
BseMII CTCAG 4 cut(s) 401, 709, 938, 950
BseNI ACTGG 1 cut(s) 1340
BseRI GAGGAG 8 cut(s) 188, 191, 641, 912, 924, 1095, 1437, 1563
BseSI GKGCMC 3 cut(s) 590, 1036, 1240
BseXI GCAGC 8 cut(s) 236, 331, 511, 656, 946, 994, 1147, 1169
BseYI CCCAGC 1 cut(s) 473
BsgI GTGCAG 1 cut(s) 243
BshFI GGCC 1 cut(s) 50
BshNI GGYRCC 2 cut(s) 185, 1235
BsiHKAI GWGCWC 3 cut(s) 703, 1036, 1573
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 4 cut(s) 158, 210, 219, 293
BslFI GGGAC 2 cut(s) 571, 702
BslI CCNNNNNNNGG 4 cut(s) 143, 1028, 1076, 1246
BsmAI GTCTC 3 cut(s) 273, 359, 786
BsmFI GGGAC 2 cut(s) 571, 702
BsmI GAATGC 1 cut(s) 516
BsnI GGCC 1 cut(s) 50
Bso31I GGTCTC 2 cut(s) 359, 786
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 5 cut(s) 590, 703, 1036, 1240, 1573
Bsp1407I TGTACA 2 cut(s) 262, 1242
Bsp143I GATC 6 cut(s) 342, 1123, 1144, 1267, 1476, 1494
BspACI CCGC 2 cut(s) 137, 161
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 4 cut(s) 400, 708, 937, 949
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 4 cut(s) 187, 208, 921, 1237
BspMI ACCTGC 2 cut(s) 765, 1223
BspPI GGATC 3 cut(s) 1131, 1262, 1502
BspT107I GGYRCC 2 cut(s) 185, 1235
BspTNI GGTCTC 2 cut(s) 359, 786
BsrDI GCAATG 1 cut(s) 1193
BsrFI RCCGGY 2 cut(s) 157, 218
BsrGI TGTACA 2 cut(s) 262, 1242
BsrI ACTGG 1 cut(s) 1340
BssAI RCCGGY 2 cut(s) 157, 218
BssECI CCNNGG 5 cut(s) 246, 323, 447, 912, 1315
BssMI GATC 6 cut(s) 342, 1123, 1144, 1267, 1476, 1494
BssNI GRCGYC 2 cut(s) 192, 256
BssT1I CCWWGG 3 cut(s) 246, 447, 1315
Bst2UI CCWGG 4 cut(s) 35, 324, 858, 1029
Bst4CI ACNGT 8 cut(s) 146, 365, 461, 562, 752, 913, 1093, 1552
Bst6I CTCTTC 2 cut(s) 1023, 1353
BstACI GRCGYC 2 cut(s) 192, 256
BstAPI GCANNNNNTGC 1 cut(s) 230
BstAUI TGTACA 2 cut(s) 262, 1242
BstC8I GCNNGC 3 cut(s) 48, 159, 311
BstDEI CTNAG 5 cut(s) 387, 695, 897, 924, 936
BstDSI CCRYGG 1 cut(s) 912
BstENI CCTNNNNNAGG 2 cut(s) 1074, 1244
BstF5I GGATG 2 cut(s) 501, 1066
BstH2I RGCGCY 1 cut(s) 1426
BstHHI GCGC 3 cut(s) 157, 687, 1425
BstKTI GATC 6 cut(s) 345, 1126, 1147, 1270, 1479, 1497
BstMAI GTCTC 3 cut(s) 273, 359, 786
BstMBI GATC 6 cut(s) 342, 1123, 1144, 1267, 1476, 1494
BstMWI GCNNNNNNNGC 6 cut(s) 215, 224, 230, 319, 1320, 1429
BstNI CCWGG 4 cut(s) 35, 324, 858, 1029
BstNSI RCATGY 2 cut(s) 269, 1299
BstSCI CCNGG 4 cut(s) 33, 322, 856, 1027
BstSLI GKGCMC 3 cut(s) 590, 1036, 1240
BstV1I GCAGC 8 cut(s) 236, 331, 511, 656, 946, 994, 1147, 1169
BstX2I RGATCY 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 1123
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 912
BtsCI GGATG 2 cut(s) 501, 1066
BtsIMutI CAGTG 2 cut(s) 567, 703
BveI ACCTGC 2 cut(s) 765, 1223
Cac8I GCNNGC 3 cut(s) 48, 159, 311
CciI TCATGA 1 cut(s) 63
CfoI GCGC 3 cut(s) 157, 687, 1425
Cfr10I RCCGGY 2 cut(s) 157, 218
Cfr13I GGNCC 1 cut(s) 1337
CseI GACGC 1 cut(s) 264
Csp6I GTAC 6 cut(s) 16, 186, 263, 854, 1243, 1553
CviQI GTAC 6 cut(s) 16, 186, 263, 854, 1243, 1553
DdeI CTNAG 5 cut(s) 387, 695, 897, 924, 936
DpnI GATC 6 cut(s) 344, 1125, 1146, 1269, 1478, 1496
DpnII GATC 6 cut(s) 342, 1123, 1144, 1267, 1476, 1494
Eam1104I CTCTTC 2 cut(s) 1023, 1353
EarI CTCTTC 2 cut(s) 1023, 1353
Ecl136II GAGCTC 1 cut(s) 1571
Eco130I CCWWGG 3 cut(s) 246, 447, 1315
Eco24I GRGCYC 1 cut(s) 1573
Eco31I GGTCTC 2 cut(s) 359, 786
Eco47I GGWCC 1 cut(s) 1337
Eco53kI GAGCTC 1 cut(s) 1571
Eco57I CTGAAG 1 cut(s) 858
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 1571
EcoNI CCTNNNNNAGG 2 cut(s) 1074, 1244
EcoRII CCWGG 4 cut(s) 33, 322, 856, 1027
EcoT14I CCWWGG 3 cut(s) 246, 447, 1315
EcoT38I GRGCYC 1 cut(s) 1573
ErhI CCWWGG 3 cut(s) 246, 447, 1315
FalI AAGNNNNNCTT 2 cut(s) 706, 738
FaqI GGGAC 2 cut(s) 571, 702
FauNDI CATATG 2 cut(s) 640, 1333
Fnu4HI GCNGC 9 cut(s) 137, 225, 320, 525, 670, 960, 1008, 1158, 1161
FokI GGATG 2 cut(s) 488, 1053
FriOI GRGCYC 1 cut(s) 1573
Fsp4HI GCNGC 9 cut(s) 137, 225, 320, 525, 670, 960, 1008, 1158, 1161
FspBI CTAG 1 cut(s) 882
FspI TGCGCA 1 cut(s) 686
GlaI GCGC 3 cut(s) 156, 686, 1424
GluI GCNGC 9 cut(s) 137, 225, 320, 525, 670, 960, 1008, 1158, 1161
GsaI CCCAGC 1 cut(s) 477
GsuI CTGGAG 1 cut(s) 642
HaeII RGCGCY 1 cut(s) 1426
HaeIII GGCC 1 cut(s) 50
HapII CCGG 4 cut(s) 158, 210, 219, 293
HgaI GACGC 1 cut(s) 264
HhaI GCGC 3 cut(s) 157, 687, 1425
Hin1I GRCGYC 2 cut(s) 192, 256
Hin6I GCGC 3 cut(s) 155, 685, 1423
HinP1I GCGC 3 cut(s) 155, 685, 1423
HinfI GANTC 6 cut(s) 30, 374, 835, 928, 1049, 1181
HpaII CCGG 4 cut(s) 158, 210, 219, 293
HphI GGTGA 6 cut(s) 100, 792, 899, 1254, 1370, 1529
Hpy166II GTNNAC 3 cut(s) 263, 1034, 1373
Hpy188I TCNGA 5 cut(s) 206, 390, 466, 635, 927
Hpy188III TCNNGA 4 cut(s) 64, 356, 1121, 1480
Hpy8I GTNNAC 3 cut(s) 263, 1034, 1373
Hpy99I CGWCG 2 cut(s) 194, 197
HpyAV CCTTC 8 cut(s) 85, 245, 385, 418, 807, 882, 1452, 1514
HpyCH4III ACNGT 8 cut(s) 146, 365, 461, 562, 752, 913, 1093, 1552
HpyCH4IV ACGT 2 cut(s) 18, 192
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 224, 230, 319, 1320, 1429
HpyF3I CTNAG 5 cut(s) 387, 695, 897, 924, 936
HpySE526I ACGT 2 cut(s) 18, 192
Hsp92I GRCGYC 2 cut(s) 192, 256
HspAI GCGC 3 cut(s) 155, 685, 1423
KpnI GGTACC 1 cut(s) 189
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Kzo9I GATC 6 cut(s) 342, 1123, 1144, 1267, 1476, 1494
LmnI GCTCC 5 cut(s) 206, 649, 706, 925, 1576
Lsp1109I GCAGC 8 cut(s) 236, 331, 511, 656, 946, 994, 1147, 1169
LweI GCATC 7 cut(s) 373, 1075, 1144, 1268, 1332, 1419, 1496
MaeI CTAG 1 cut(s) 882
MaeII ACGT 2 cut(s) 18, 192
MaeIII GTNAC 2 cut(s) 562, 752
MalI GATC 6 cut(s) 344, 1125, 1146, 1269, 1478, 1496
MboI GATC 6 cut(s) 342, 1123, 1144, 1267, 1476, 1494
MboII GAAGA 6 cut(s) 590, 1010, 1106, 1109, 1118, 1340
MfeI CAATTG 1 cut(s) 963
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 5 cut(s) 590, 703, 1036, 1240, 1573
MluCI AATT 7 cut(s) 300, 454, 545, 573, 946, 963, 1171
MlyI GAGTC 1 cut(s) 39
MmeI TCCRAC 2 cut(s) 184, 723
MreI CGCCGGCG 1 cut(s) 157
MroNI GCCGGC 1 cut(s) 157
MseI TTAA 3 cut(s) 117, 1131, 1362
MslI CAYNNNNRTG 1 cut(s) 777
MspA1I CMGCKG 1 cut(s) 1160
MspI CCGG 4 cut(s) 158, 210, 219, 293
MspR9I CCNGG 4 cut(s) 35, 324, 858, 1029
MunI CAATTG 1 cut(s) 963
Mva1269I GAATGC 1 cut(s) 516
MvaI CCWGG 4 cut(s) 35, 324, 858, 1029
MwoI GCNNNNNNNGC 6 cut(s) 215, 224, 230, 319, 1320, 1429
NaeI GCCGGC 1 cut(s) 159
NdeI CATATG 2 cut(s) 640, 1333
NdeII GATC 6 cut(s) 342, 1123, 1144, 1267, 1476, 1494
NgoMIV GCCGGC 1 cut(s) 157
NlaIV GGNNCC 4 cut(s) 187, 208, 921, 1237
NmuCI GTSAC 2 cut(s) 562, 752
NsbI TGCGCA 1 cut(s) 686
NspI RCATGY 2 cut(s) 269, 1299
PaeR7I CTCGAG 1 cut(s) 356
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 1 cut(s) 1223
PciI ACATGT 1 cut(s) 265
PctI GAATGC 1 cut(s) 516
PdiI GCCGGC 1 cut(s) 159
PfeI GAWTC 5 cut(s) 374, 835, 928, 1049, 1181
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 9 cut(s) 138, 226, 321, 526, 671, 961, 1009, 1159, 1162
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
PscI ACATGT 1 cut(s) 265
PsiI TTATAA 1 cut(s) 1511
Psp124BI GAGCTC 1 cut(s) 1573
Psp6I CCWGG 4 cut(s) 33, 322, 856, 1027
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 4 cut(s) 33, 322, 856, 1027
PspN4I GGNNCC 4 cut(s) 187, 208, 921, 1237
PspPI GGNCC 1 cut(s) 1337
PsuI RGATCY 1 cut(s) 1123
PsyI GACNNNGTC 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 1160
RsaI GTAC 6 cut(s) 17, 187, 264, 855, 1244, 1554
RsaNI GTAC 6 cut(s) 16, 186, 263, 854, 1243, 1553
RseI CAYNNNNRTG 1 cut(s) 777
SacI GAGCTC 1 cut(s) 1573
SaqAI TTAA 3 cut(s) 117, 1131, 1362
SatI GCNGC 9 cut(s) 137, 225, 320, 525, 670, 960, 1008, 1158, 1161
Sau3AI GATC 6 cut(s) 342, 1123, 1144, 1267, 1476, 1494
Sau96I GGNCC 1 cut(s) 1337
SchI GAGTC 1 cut(s) 39
ScrFI CCNGG 4 cut(s) 35, 324, 858, 1029
SduI GDGCHC 5 cut(s) 590, 703, 1036, 1240, 1573
SfaNI GCATC 7 cut(s) 373, 1075, 1144, 1268, 1332, 1419, 1496
Sfr274I CTCGAG 1 cut(s) 356
SgrAI CRCCGGYG 1 cut(s) 157
SinI GGWCC 1 cut(s) 1337
SlaI CTCGAG 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 4 cut(s) 283, 335, 356, 1480
SmoI CTYRAG 4 cut(s) 283, 335, 356, 1480
Sse9I AATT 7 cut(s) 300, 454, 545, 573, 946, 963, 1171
SsiI CCGC 2 cut(s) 137, 161
SspMI CTAG 1 cut(s) 882
SstI GAGCTC 1 cut(s) 1573
StyD4I CCNGG 4 cut(s) 33, 322, 856, 1027
StyI CCWWGG 3 cut(s) 246, 447, 1315
TaaI ACNGT 8 cut(s) 146, 365, 461, 562, 752, 913, 1093, 1552
TaiI ACGT 2 cut(s) 21, 195
TaqI TCGA 3 cut(s) 273, 345, 357
TasI AATT 7 cut(s) 300, 454, 545, 573, 946, 963, 1171
TatI WGTACW 2 cut(s) 262, 1242
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 5 cut(s) 374, 835, 928, 1049, 1181
Tru1I TTAA 3 cut(s) 117, 1131, 1362
Tru9I TTAA 3 cut(s) 117, 1131, 1362
TscAI CASTG 2 cut(s) 567, 703
TseFI GTSAC 2 cut(s) 562, 752
TseI GCWGC 8 cut(s) 224, 319, 524, 669, 959, 1007, 1157, 1160
Tsp45I GTSAC 2 cut(s) 562, 752
TspDTI ATGAA 5 cut(s) 49, 387, 490, 1041, 1320
TspRI CASTG 2 cut(s) 567, 703
Tth111I GACNNNGTC 1 cut(s) 194
VneI GTGCAC 1 cut(s) 1032
VpaK11BI GGWCC 1 cut(s) 1337
XagI CCTNNNNNAGG 2 cut(s) 1074, 1244
XceI RCATGY 2 cut(s) 269, 1299
XcmI CCANNNNNNNNNTGG 1 cut(s) 1225
XhoI CTCGAG 1 cut(s) 356
XspI CTAG 1 cut(s) 882
ZraI GACGTC 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.