Prupe.8G156600_v2.0.a1

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000108
Physical Location & Seq
Reverse (-)
16737550 .. 16741257
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G156600.1

Sequence Viewer

Length: 1572 bp
ATGGTTTACCAGAATATTTTTGGTGCAGTCATAGCTTCATTGCTGGGGTTTGCTTTCTTGTACAGACTAAGAGGGAAGAAGAAAGCCAGAGCTTTAGTGAAGACTGATCAGAATGGGAACTGTTTGAAGAGCTCTGAAAATGGAATTTGTGAGGCGAAGATAAGTACAAGTACGGATATTATCATTGTTGGTGCTGGGGTTGTTGGTTCGGCACTTGCTTACACTCTTGGGAAGGAAGGGCGCCGAGTGCATGTGATTGAAAGAGACTTGGCTGAGCCAGATAGAATCGTTGGTGAACTTCTACAACCAGGGGGCTACCTGAAATTAATTGAGTTGGGCCTTGAGGATTGTGTGAGTGAGATTGATGCTCAGCGGGTTTTGGGATATGCTCTTTACAAGGATGGGAAAAGTACCAAGTTGCCATATCCTTTGGAAAACTTCCATCCAAATGTGGCTGGGAGAAGCTTTCACCATGGGCGTTTCATTCAAAGGATGCGTCAAAAAGCTTCATCTCTTCCCAATGTAAGATTGGAACAAGGAACAGTGACATCTTTGCTTGAAGAAAAAGGTACTGTCAAGGGGGTGCAGTATAAATCCAAAGGTGGTGACCTAGAACTCACTGCACATGCTCCTCTCACGATAGTATGCGATGGATGCTACTCAAACCTGCGGCGCTCTCTCTGCAATCCTAAGGTTGATATTCCTTCTTGTTTTGTTGGTTTGGTTCTGGAGAATTGCCAACTTCCATACACAAACCATGGGCATGTAATTTTGGCAGATCCTTCACCGATACTGTTTTATCCTATCAGCAGCACAGAGATTCGGTGTCTGGTTGATGTACCAGGCCAAAAAGTACCTTCCATTTCTGGTGGTGAAATGGCTCACTACTTGAAAACCAAAGTGGCACCCCAGGTTCCTCCTGAGATGTATGCTGCTTTCATGGCTGCTACTGATAAAGGAAACATAAAATCCATGCCCAATAGGAGCATGCCTGCTGCTCCTCATCCTAGGCCTGGTGCCCTTCTAATGGGGGATGCTGTCAACATGAGGCATCCTTTAACTGGAGGAGGCATGACTGTGGCTCTATCAGACATTGTTGTTCTGAGGAACCTTCTGAGACCTCTGCACAATCTGAATGATGCACCTGCCCTTTGCAAATACCTCGAATCCTTCTATACCCTTCGCAAGCCTGTGGCTTCTACCATAAACACATTGGCAGGTGCACTATACAAGGTGTTCTGTGCATCCCCTGACCCGGCAAGGAAGGAAATGCGCCAAGCATGCTTCGACTACTTGAGCCTCGGAGGGGTCTTCTCAAATGGACCTCTGGCGATTCTCTCCGGTCTAAACCCTCGTCCATTGAGCTTGGTTCTTCACTTCTTTGCTGTGGCTGTCTATGCTGTTGGGCGCTTGTTGATTCCATTTCCTTCACCCAAACGCATTTGGGTTGGAGCTAGACTGATTTTGGGTGCATCAGGGATCATATTCCCCATAATCAACTCTGAAGGAGTAAGACAAATGTTCTTCCCTGTGACAGTGCTATCATATTACAGAGCTCCTCCAGTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

524

Amino Acids

56.88

Weight (kDa)

9.35

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1153, 1208
Acc36I ACCTGC 3 cut(s) 675, 1153, 1208
AccB1I GGYRCC 3 cut(s) 240, 904, 1016
AciI CCGC 2 cut(s) 373, 670
AclWI GGATC 2 cut(s) 773, 1487
AcsI RAATTY 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 1524
AcyI GRCGYC 1 cut(s) 241
AfaI GTAC 7 cut(s) 62, 166, 172, 412, 571, 840, 855
AfiI CCNNNNNNNGG 5 cut(s) 1013, 1027, 1061, 1255, 1306
AgsI TTSAA 5 cut(s) 127, 260, 488, 560, 892
AjnI CCWGG 4 cut(s) 307, 841, 909, 1012
AluBI AGCT 8 cut(s) 35, 92, 132, 465, 506, 1365, 1454, 1556
AluI AGCT 8 cut(s) 35, 92, 132, 465, 506, 1365, 1454, 1556
Alw21I GWGCWC 3 cut(s) 134, 1225, 1558
Alw26I GTCTC 2 cut(s) 258, 1111
Alw44I GTGCAC 1 cut(s) 1221
AlwI GGATC 2 cut(s) 773, 1487
AoxI GGCC 3 cut(s) 337, 844, 1010
ApaLI GTGCAC 1 cut(s) 1221
ApeKI GCWGC 4 cut(s) 810, 932, 944, 995
ApoI RAATTY 1 cut(s) 144
ArsI GACNNNNNNTTYG 2 cut(s) 481, 513
AseI ATTAAT 1 cut(s) 326
AspA2I CCTAGG 1 cut(s) 1007
AspLEI GCGC 4 cut(s) 243, 675, 1275, 1410
AspS9I GGNCC 2 cut(s) 337, 1322
AsuC2I CCSGG 1 cut(s) 1256
AsuHPI GGTGA 6 cut(s) 305, 461, 617, 777, 884, 1422
AvaII GGWCC 1 cut(s) 1322
AvrII CCTAGG 1 cut(s) 1007
AxyI CCTNAGG 1 cut(s) 690
BaeGI GKGCMC 2 cut(s) 1021, 1225
BanI GGYRCC 3 cut(s) 240, 904, 1016
BanII GRGCYC 2 cut(s) 134, 1558
BbsI GAAGAC 2 cut(s) 107, 1303
Bbv12I GWGCWC 3 cut(s) 134, 1225, 1558
BbvI GCAGC 4 cut(s) 822, 919, 931, 982
BccI CCATC 3 cut(s) 395, 450, 644
BcgI CGANNNNNNTGC 2 cut(s) 1144, 1178
BciT130I CCWGG 4 cut(s) 309, 843, 911, 1014
BclI TGATCA 1 cut(s) 106
BcnI CCSGG 1 cut(s) 1256
BcoDI GTCTC 2 cut(s) 258, 1111
BfaI CTAG 3 cut(s) 611, 1008, 1455
BfoI RGCGCY 3 cut(s) 244, 676, 1411
BfuAI ACCTGC 3 cut(s) 675, 1153, 1208
BisI GCNGC 5 cut(s) 671, 811, 933, 945, 996
BlnI CCTAGG 1 cut(s) 1007
BlpI GCTNAGC 2 cut(s) 273, 369
BlsI GCNGC 5 cut(s) 672, 812, 934, 946, 997
Bme1390I CCNGG 5 cut(s) 309, 843, 911, 1014, 1256
Bme18I GGWCC 1 cut(s) 1322
BmgT120I GGNCC 2 cut(s) 337, 1322
BmiI GGNNCC 5 cut(s) 242, 906, 915, 1018, 1109
BmrFI CCNGG 5 cut(s) 309, 843, 911, 1014, 1256
BmsI GCATC 8 cut(s) 355, 483, 644, 1024, 1060, 1129, 1253, 1481
BpiI GAAGAC 2 cut(s) 107, 1303
BpmI CTGGAG 3 cut(s) 749, 1083, 1545
Bpu1102I GCTNAGC 2 cut(s) 273, 369
BpuEI CTTGAG 2 cut(s) 362, 1315
BpuMI CCSGG 1 cut(s) 1256
BsaHI GRCGYC 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 1111
BsaJI CCNNGG 6 cut(s) 308, 472, 757, 909, 1007, 1300
BsaWI WCCGGW 1 cut(s) 1340
Bsc4I CCNNNNNNNGG 5 cut(s) 1013, 1027, 1061, 1255, 1306
Bse1I ACTGG 2 cut(s) 1066, 1562
Bse21I CCTNAGG 1 cut(s) 690
Bse3DI GCAATG 1 cut(s) 38
BseBI CCWGG 4 cut(s) 309, 843, 911, 1014
BseDI CCNNGG 6 cut(s) 308, 472, 757, 909, 1007, 1300
BseGI GGATG 8 cut(s) 406, 442, 498, 659, 1003, 1039, 1051, 1244
BseLI CCNNNNNNNGG 5 cut(s) 1013, 1027, 1061, 1255, 1306
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 5 cut(s) 264, 383, 912, 1094, 1106
BseNI ACTGG 2 cut(s) 1066, 1562
BseRI GAGGAG 4 cut(s) 621, 990, 1080, 1548
BseSI GKGCMC 2 cut(s) 1021, 1225
BseXI GCAGC 4 cut(s) 822, 919, 931, 982
BseYI CCCAGC 3 cut(s) 43, 194, 455
BsgI GTGCAG 4 cut(s) 45, 605, 606, 1109
BshFI GGCC 3 cut(s) 339, 846, 1012
BshNI GGYRCC 3 cut(s) 240, 904, 1016
BsiHKAI GWGCWC 3 cut(s) 134, 1225, 1558
BsiSI CCGG 2 cut(s) 1256, 1341
BslI CCNNNNNNNGG 5 cut(s) 1013, 1027, 1061, 1255, 1306
BsmAI GTCTC 2 cut(s) 258, 1111
BsnI GGCC 3 cut(s) 339, 846, 1012
Bso31I GGTCTC 1 cut(s) 1111
Bsp1286I GDGCHC 4 cut(s) 134, 1021, 1225, 1558
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 3 cut(s) 106, 778, 1479
Bsp1720I GCTNAGC 2 cut(s) 273, 369
Bsp19I CCATGG 2 cut(s) 472, 757
BspACI CCGC 2 cut(s) 373, 670
BspANI GGCC 3 cut(s) 339, 846, 1012
BspCNI CTCAG 5 cut(s) 265, 382, 913, 1095, 1107
BspLI GGNNCC 5 cut(s) 242, 906, 915, 1018, 1109
BspMI ACCTGC 3 cut(s) 675, 1153, 1208
BspPI GGATC 2 cut(s) 773, 1487
BspQI GCTCTTC 1 cut(s) 122
BspT107I GGYRCC 3 cut(s) 240, 904, 1016
BspTNI GGTCTC 1 cut(s) 1111
BsrDI GCAATG 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 2 cut(s) 1066, 1562
BssECI CCNNGG 6 cut(s) 308, 472, 757, 909, 1007, 1300
BssMI GATC 3 cut(s) 106, 778, 1479
BssNI GRCGYC 1 cut(s) 241
BssT1I CCWWGG 3 cut(s) 472, 757, 1007
Bst2UI CCWGG 4 cut(s) 309, 843, 911, 1014
Bst4CI ACNGT 6 cut(s) 122, 544, 574, 795, 1078, 1537
Bst6I CTCTTC 2 cut(s) 122, 519
BstACI GRCGYC 1 cut(s) 241
BstAUI TGTACA 1 cut(s) 60
BstC8I GCNNGC 4 cut(s) 989, 993, 1187, 1282
BstDEI CTNAG 7 cut(s) 68, 273, 369, 690, 921, 1103, 1115
BstDSI CCRYGG 2 cut(s) 472, 757
BstEII GGTNACC 1 cut(s) 605
BstF5I GGATG 8 cut(s) 406, 442, 498, 659, 1003, 1039, 1051, 1244
BstH2I RGCGCY 3 cut(s) 244, 676, 1411
BstHHI GCGC 4 cut(s) 243, 675, 1275, 1410
BstKTI GATC 3 cut(s) 109, 781, 1482
BstMAI GTCTC 2 cut(s) 258, 1111
BstMBI GATC 3 cut(s) 106, 778, 1479
BstMWI GCNNNNNNNGC 7 cut(s) 32, 247, 654, 681, 941, 1281, 1397
BstNI CCWGG 4 cut(s) 309, 843, 911, 1014
BstNSI RCATGY 5 cut(s) 254, 629, 767, 991, 1284
BstPI GGTNACC 1 cut(s) 605
BstSCI CCNGG 5 cut(s) 307, 841, 909, 1012, 1254
BstSLI GKGCMC 2 cut(s) 1021, 1225
BstV1I GCAGC 4 cut(s) 822, 919, 931, 982
BstV2I GAAGAC 2 cut(s) 107, 1303
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
Bsu36I CCTNAGG 1 cut(s) 690
BsuRI GGCC 3 cut(s) 339, 846, 1012
BtgI CCRYGG 2 cut(s) 472, 757
BtgZI GCGATG 1 cut(s) 663
BtsCI GGATG 8 cut(s) 406, 442, 498, 659, 1003, 1039, 1051, 1244
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 3 cut(s) 549, 618, 1542
BveI ACCTGC 3 cut(s) 675, 1153, 1208
Cac8I GCNNGC 4 cut(s) 989, 993, 1187, 1282
CfoI GCGC 4 cut(s) 243, 675, 1275, 1410
Cfr13I GGNCC 2 cut(s) 337, 1322
CseI GACGC 1 cut(s) 485
Csp6I GTAC 7 cut(s) 61, 165, 171, 411, 570, 839, 854
CviQI GTAC 7 cut(s) 61, 165, 171, 411, 570, 839, 854
DdeI CTNAG 7 cut(s) 68, 273, 369, 690, 921, 1103, 1115
DinI GGCGCC 1 cut(s) 242
DpnI GATC 3 cut(s) 108, 780, 1481
DpnII GATC 3 cut(s) 106, 778, 1479
Eam1104I CTCTTC 2 cut(s) 122, 519
EarI CTCTTC 2 cut(s) 122, 519
Ecl136II GAGCTC 2 cut(s) 132, 1556
Eco130I CCWWGG 3 cut(s) 472, 757, 1007
Eco147I AGGCCT 1 cut(s) 1012
Eco24I GRGCYC 2 cut(s) 134, 1558
Eco31I GGTCTC 1 cut(s) 1111
Eco47I GGWCC 1 cut(s) 1322
Eco53kI GAGCTC 2 cut(s) 132, 1556
Eco57I CTGAAG 1 cut(s) 1524
Eco81I CCTNAGG 1 cut(s) 690
Eco91I GGTNACC 1 cut(s) 605
EcoICRI GAGCTC 2 cut(s) 132, 1556
EcoO65I GGTNACC 1 cut(s) 605
EcoRII CCWGG 4 cut(s) 307, 841, 909, 1012
EcoT14I CCWWGG 3 cut(s) 472, 757, 1007
EcoT38I GRGCYC 2 cut(s) 134, 1558
EgeI GGCGCC 1 cut(s) 242
EheI GGCGCC 1 cut(s) 242
ErhI CCWWGG 3 cut(s) 472, 757, 1007
FauI CCCGC 1 cut(s) 366
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 5 cut(s) 671, 811, 933, 945, 996
FokI GGATG 8 cut(s) 413, 429, 505, 666, 990, 1038, 1046, 1231
FriOI GRGCYC 2 cut(s) 134, 1558
Fsp4HI GCNGC 5 cut(s) 671, 811, 933, 945, 996
FspBI CTAG 3 cut(s) 611, 1008, 1455
GlaI GCGC 4 cut(s) 242, 674, 1274, 1409
GluI GCNGC 5 cut(s) 671, 811, 933, 945, 996
GsaI CCCAGC 3 cut(s) 47, 198, 459
GsuI CTGGAG 3 cut(s) 749, 1083, 1545
HaeII RGCGCY 3 cut(s) 244, 676, 1411
HaeIII GGCC 3 cut(s) 339, 846, 1012
HapII CCGG 2 cut(s) 1256, 1341
HgaI GACGC 1 cut(s) 485
HhaI GCGC 4 cut(s) 243, 675, 1275, 1410
Hin1I GRCGYC 1 cut(s) 241
Hin6I GCGC 4 cut(s) 241, 673, 1273, 1408
HinP1I GCGC 4 cut(s) 241, 673, 1273, 1408
HincII GTYRAC 1 cut(s) 1042
HindII GTYRAC 1 cut(s) 1042
HindIII AAGCTT 2 cut(s) 463, 504
HinfI GANTC 5 cut(s) 285, 820, 1166, 1333, 1417
HpaII CCGG 2 cut(s) 1256, 1341
HphI GGTGA 6 cut(s) 305, 461, 617, 777, 884, 1422
Hpy166II GTNNAC 4 cut(s) 7, 296, 1042, 1223
Hpy188I TCNGA 8 cut(s) 111, 136, 1090, 1104, 1116, 1134, 1304, 1504
Hpy188III TCNNGA 3 cut(s) 637, 728, 920
Hpy8I GTNNAC 4 cut(s) 7, 296, 1042, 1223
HpyCH4III ACNGT 6 cut(s) 122, 544, 574, 795, 1078, 1537
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 247, 654, 681, 941, 1281, 1397
HpyF3I CTNAG 7 cut(s) 68, 273, 369, 690, 921, 1103, 1115
Hsp92I GRCGYC 1 cut(s) 241
HspAI GCGC 4 cut(s) 241, 673, 1273, 1408
KasI GGCGCC 1 cut(s) 240
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 3 cut(s) 106, 778, 1479
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 5 cut(s) 634, 984, 1003, 1451, 1561
Lsp1109I GCAGC 4 cut(s) 822, 919, 931, 982
LweI GCATC 8 cut(s) 355, 483, 644, 1024, 1060, 1129, 1253, 1481
MaeI CTAG 3 cut(s) 611, 1008, 1455
MaeIII GTNAC 3 cut(s) 544, 605, 1531
MalI GATC 3 cut(s) 108, 780, 1481
MboI GATC 3 cut(s) 106, 778, 1479
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 4 cut(s) 134, 1021, 1225, 1558
MluCI AATT 6 cut(s) 144, 323, 327, 733, 768, 1567
Mly113I GGCGCC 1 cut(s) 241
MmeI TCCRAC 1 cut(s) 1429
MseI TTAA 4 cut(s) 326, 1058, 1566, 1570
MslI CAYNNNNRTG 3 cut(s) 447, 762, 1076
MspA1I CMGCKG 1 cut(s) 373
MspI CCGG 2 cut(s) 1256, 1341
MspR9I CCNGG 5 cut(s) 309, 843, 911, 1014, 1256
MvaI CCWGG 4 cut(s) 309, 843, 911, 1014
MwoI GCNNNNNNNGC 7 cut(s) 32, 247, 654, 681, 941, 1281, 1397
NarI GGCGCC 1 cut(s) 241
NciI CCSGG 1 cut(s) 1256
NcoI CCATGG 2 cut(s) 472, 757
NdeII GATC 3 cut(s) 106, 778, 1479
NlaIV GGNNCC 5 cut(s) 242, 906, 915, 1018, 1109
NmeAIII GCCGAG 1 cut(s) 269
NmuCI GTSAC 3 cut(s) 544, 605, 1531
NspI RCATGY 5 cut(s) 254, 629, 767, 991, 1284
PacI TTAATTAA 1 cut(s) 1570
PaeI GCATGC 2 cut(s) 991, 1284
PaqCI CACCTGC 2 cut(s) 1153, 1208
PceI AGGCCT 1 cut(s) 1012
PciSI GCTCTTC 1 cut(s) 122
PfeI GAWTC 5 cut(s) 285, 820, 1166, 1333, 1417
PkrI GCNGC 5 cut(s) 672, 812, 934, 946, 997
PluTI GGCGCC 1 cut(s) 244
PshBI ATTAAT 1 cut(s) 326
Psp124BI GAGCTC 2 cut(s) 134, 1558
Psp6I CCWGG 4 cut(s) 307, 841, 909, 1012
PspEI GGTNACC 1 cut(s) 605
PspFI CCCAGC 3 cut(s) 43, 194, 455
PspGI CCWGG 4 cut(s) 307, 841, 909, 1012
PspN4I GGNNCC 5 cut(s) 242, 906, 915, 1018, 1109
PspPI GGNCC 2 cut(s) 337, 1322
PsuI RGATCY 1 cut(s) 778
RsaI GTAC 7 cut(s) 62, 166, 172, 412, 571, 840, 855
RsaNI GTAC 7 cut(s) 61, 165, 171, 411, 570, 839, 854
RseI CAYNNNNRTG 3 cut(s) 447, 762, 1076
SacI GAGCTC 2 cut(s) 134, 1558
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 4 cut(s) 326, 1058, 1566, 1570
SatI GCNGC 5 cut(s) 671, 811, 933, 945, 996
Sau3AI GATC 3 cut(s) 106, 778, 1479
Sau96I GGNCC 2 cut(s) 337, 1322
ScrFI CCNGG 5 cut(s) 309, 843, 911, 1014, 1256
SduI GDGCHC 4 cut(s) 134, 1021, 1225, 1558
SfaNI GCATC 8 cut(s) 355, 483, 644, 1024, 1060, 1129, 1253, 1481
SfoI GGCGCC 1 cut(s) 242
SinI GGWCC 1 cut(s) 1322
SmiMI CAYNNNNRTG 3 cut(s) 447, 762, 1076
SmlI CTYRAG 2 cut(s) 341, 1294
SmoI CTYRAG 2 cut(s) 341, 1294
SphI GCATGC 2 cut(s) 991, 1284
Sse9I AATT 6 cut(s) 144, 323, 327, 733, 768, 1567
SseBI AGGCCT 1 cut(s) 1012
SsiI CCGC 2 cut(s) 373, 670
SspDI GGCGCC 1 cut(s) 240
SspI AATATT 1 cut(s) 16
SspMI CTAG 3 cut(s) 611, 1008, 1455
SstI GAGCTC 2 cut(s) 134, 1558
StuI AGGCCT 1 cut(s) 1012
StyD4I CCNGG 5 cut(s) 307, 841, 909, 1012, 1254
StyI CCWWGG 3 cut(s) 472, 757, 1007
TaaI ACNGT 6 cut(s) 122, 544, 574, 795, 1078, 1537
TaqI TCGA 2 cut(s) 1164, 1287
TasI AATT 6 cut(s) 144, 323, 327, 733, 768, 1567
TatI WGTACW 2 cut(s) 60, 164
TauI GCSGC 1 cut(s) 673
TfiI GAWTC 5 cut(s) 285, 820, 1166, 1333, 1417
Tru1I TTAA 4 cut(s) 326, 1058, 1566, 1570
Tru9I TTAA 4 cut(s) 326, 1058, 1566, 1570
TscAI CASTG 3 cut(s) 549, 625, 1542
TseFI GTSAC 3 cut(s) 544, 605, 1531
TseI GCWGC 4 cut(s) 810, 932, 944, 995
Tsp45I GTSAC 3 cut(s) 544, 605, 1531
TspDTI ATGAA 4 cut(s) 27, 472, 498, 928
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 3 cut(s) 549, 625, 1542
VneI GTGCAC 1 cut(s) 1221
VpaK11BI GGWCC 1 cut(s) 1322
VspI ATTAAT 1 cut(s) 326
XapI RAATTY 1 cut(s) 144
XceI RCATGY 5 cut(s) 254, 629, 767, 991, 1284
XcmI CCANNNNNNNNNTGG 3 cut(s) 17, 526, 1210
XmaJI CCTAGG 1 cut(s) 1007
XspI CTAG 3 cut(s) 611, 1008, 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.