Rmu_sc0009831.1_g000002

Squalene epoxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009831.1
OG0000108
Physical Location & Seq
Forward (+)
3681 .. 11614
7934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009831.1_g000002.1.cds

Sequence Viewer

Length: 3156 bp
atgatcatcagtactactcttggtggaaacaaaaaggtcaagcctttaagtggagcaaagagcaatgggtttggaaacgatgcgtttcagccggaaatggaggaggagaaaagtaccgacgtcgtcattgtcggtgccggagttgctggtgcagctcttgcttacactcttgccaaggaaggacgtcgtgtccatgtcattgaaagagacttgaccgaaccagatagaattgttggtgagctattgcagcctggaggttatctcaagttgattgagttgggtcttgaggaatgtgcaaatgagtccattgatgctcagaaggtgtttggatatgctctatacaaagatggaaaggatacaaaactgacttatcccttggaaaaatacagttcagatgtggctgggagaagtttccacaatgggcgtttcatccaaagaatgcgtgaaaaagctgtaactctttcaaatgtgaaattggaacaaggatcagtgacaacattgatcgaggaaaagggcattgtcaaaggggtgatttacaagaacaaggctggagaggagatgagaacatatgctccactgacaatcgtgtgtgatggctgcttttcaaatctgcgcaaatcactcagtgatccaaaggttgagagtccctcttgttttgttggtttgatcttggaaaattgtgacctgccacacgcaaatcatggacatgtcattttgggagacccttcacccatcctgttttatcctatcagtagcactgagattcgctgtttggtcgatgtacctggaacaaaagtaccttcagtagctagtggtgaaatggctagctatctgaaaactgttgtggctcctcaggttcccccacagctgtacaatgcttttatggttgcagtagagaaaggaaacattagatccatgcaaaacaaaagcatggctgctaatcctgttcccactcctggtgcaattttgttaggggattcattcaacatgaggcatcctttaacaggaggaggaatgaccgtggctctttcagacattgttcttctccgcgatcttcttagacccctgcgtgatctcaatgatgcaccagccttgtgcaattaccttgaatcattctacacacttcggaagcctgtgtcatctaccataaacacattggcaggtgctttgtacaaggttttttgtgcatcacctgatccagcaagacaggaaatgcgtgaagcatgttttggctatttgagccttggaggcatctgttcatatggaccagtatctcttctctctggtcttaacccccgtccactgcacttgtttctccatttctttgctgttgctatatatggcgtagggcgcttaatgtttccattcccttcacctaaaagcatgtggcttggggctagattgatcttgagtgcatcaggcatcatattcccgattataaagggtgaaggagttagacagattgtgaaattggaacaaggatcagtgacaacattgatcgaggaaaagggcattgtcaaaggggtgatttacaagaacaaggctggagaggagatgagaacatatgctccactgacaatcgtgtgtgatggctgcttttcaaatctgcgcaaatcactcagtgatccaaaggttgaaagtccctcttgttttgttggtttgatcttggaaaattgtgagctgccacacgcaaatcatggacatgtcattttgggagacccttcacccatcctgttttatcctatcagtagcaccgagattcgctgtttggtcgatgtacctggaacaaaagtaccttcagtagctagtggtgaaatggctagctatctgaaaactgttgtggctcctcaggttcccccacagctgtacaatgcttttatggttgcagtagagaaaggaaacattagatccatgcaaaacaaaagcatggctgctaatcctgttcccactcctggtgcaattttgttaggggattcattcaacatgaggcatcctttaacaggaggaggaatgaccgtggctctttcagacattgttcttctccgcgatcttcttagacccctgcgtgatctcaatgatgcaccagccttgtgcaattaccttgaatcattctacacacttcggaagcctgtgtcatctaccataaacacattggcaggtgctttgtacaaggttttttgtgcatcacctgatccagcaagacaggaaatgcgtgaagcatgttttggctatttgagccttggaggcatctgttcatatggaccagtatctcttctctctggtcttaacccccgtccactgcacttgtttctccatttctttgctgttgctatatatggcgtagggcgcttaatgtttccattcccttcacctaaaagcatgtggcttggggctagattgatcttgagtgcatcaggcatcatattcccgattataaagggtgaaggagttagacagatgttctttcctgcaacatcgttattcaagctgagtccctcttgttttgttggtttgatcttggaaaattgtgacctgccacacgcaaatcatggacatgtcattttgggagacccttcacccatcctgttttatcctatcagtagcactgagattcgctgtttggtcgatgtacctggaacaaaagtaccttcagtagctagtggtgaaatggctagctatctgaaaactgttgtggctcctcaggttcccccacagctgtacaatgcttttatggttgcagtagagaaaggaaacattagatccatgcaaaacaaaagcatggctgctaatcctgttcccactcctggtgcaattttgttaggggattcattcaacatgaggcatcctttaacaggaggaggaatgaccgtggctctttcagacattgttcttctccgcgatcttcttagacccctgcgtgatctcaatgatgcaccagccttgtgcaattaccttgaatcattctacacacttcggaagcctgtgtcatctaccataaacacattggcaggtgctttgtacaaggttttttgtgcatcacctgatccagcaagacaggaaatgcgtgaagcatgtttcggctatttgagccttggaggcatctgttcatatggaccaggatcatttggttttggtggtgggcattttttctcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

1051

Amino Acids

113.52

Weight (kDa)

8.45

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1439, 2435
AarI CACCTGC 3 cut(s) 1151, 2147, 2999
AatII GACGTC 2 cut(s) 123, 187
Acc16I TGCGCA 2 cut(s) 614, 1610
Acc36I ACCTGC 5 cut(s) 693, 1151, 2147, 2541, 2999
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 3 cut(s) 1050, 2046, 2898
AciI CCGC 3 cut(s) 1048, 2044, 2896
AcuI CTGAAG 3 cut(s) 786, 1782, 2634
AcyI GRCGYC 2 cut(s) 120, 184
AdeI CACNNNGTG 2 cut(s) 626, 1622
AfiI CCNNNNNNNGG 7 cut(s) 50, 956, 1004, 1952, 2000, 2804, 2852
AflIII ACRYGT 3 cut(s) 706, 1702, 2554
AhdI GACNNNNNGTC 1 cut(s) 188
AjnI CCWGG 8 cut(s) 250, 784, 955, 1780, 1951, 2632, 2803, 3115
AjuI GAANNNNNNNTTGG 4 cut(s) 1212, 1244, 2208, 2240
AloI GAACNNNNNNTCC 4 cut(s) 556, 588, 1552, 1584
Alw26I GTCTC 4 cut(s) 201, 714, 1710, 2562
ApeKI GCWGC 8 cut(s) 152, 247, 597, 935, 1593, 1681, 1931, 2783
AspLEI GCGC 4 cut(s) 615, 1353, 1611, 2349
AspS9I GGNCC 3 cut(s) 1265, 2261, 3113
AsuNHI GCTAGC 3 cut(s) 824, 1820, 2672
AvaII GGWCC 3 cut(s) 1265, 2261, 3113
AxyI CCTNAGG 3 cut(s) 852, 1848, 2700
BaeI ACNNNNGTAYC 6 cut(s) 780, 813, 1776, 1809, 2628, 2661
BanI GGYRCC 1 cut(s) 134
BbvI GCAGC 8 cut(s) 164, 259, 584, 922, 1580, 1668, 1918, 2770
BccI CCATC 6 cut(s) 341, 587, 740, 1583, 1736, 2588
BciT130I CCWGG 8 cut(s) 252, 786, 957, 1782, 1953, 2634, 2805, 3117
BciVI GTATCC 1 cut(s) 349
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 4 cut(s) 201, 714, 1710, 2562
BfaI CTAG 8 cut(s) 810, 825, 1398, 1806, 1821, 2394, 2658, 2673
BfoI RGCGCY 2 cut(s) 1354, 2350
BfuAI ACCTGC 5 cut(s) 693, 1151, 2147, 2541, 2999
BfuI GTATCC 1 cut(s) 349
BglI GCCNNNNNGGC 3 cut(s) 1248, 2244, 3096
BisI GCNGC 8 cut(s) 153, 248, 598, 936, 1594, 1682, 1932, 2784
BlsI GCNGC 8 cut(s) 154, 249, 599, 937, 1595, 1683, 1933, 2785
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 8 cut(s) 252, 786, 957, 1782, 1953, 2634, 2805, 3117
Bme18I GGWCC 3 cut(s) 1265, 2261, 3113
BmeRI GACNNNNNGTC 1 cut(s) 188
BmgT120I GGNCC 3 cut(s) 1265, 2261, 3113
BmiI GGNNCC 7 cut(s) 136, 849, 858, 1845, 1854, 2697, 2706
BmrFI CCNGG 8 cut(s) 252, 786, 957, 1782, 1953, 2634, 2805, 3117
BmtI GCTAGC 3 cut(s) 828, 1824, 2676
BplI GAGNNNNNCTC 4 cut(s) 246, 278, 632, 664
BpmI CTGGAG 3 cut(s) 273, 570, 1566
BpuEI CTTGAG 4 cut(s) 248, 305, 1429, 2425
BsaHI GRCGYC 2 cut(s) 120, 184
BsaI GGTCTC 3 cut(s) 714, 1710, 2562
BsaJI CCNNGG 8 cut(s) 174, 375, 1020, 1243, 2016, 2239, 2868, 3091
BsaXI ACNNNNNCTCC 4 cut(s) 556, 586, 1552, 1582
Bsc4I CCNNNNNNNGG 7 cut(s) 50, 956, 1004, 1952, 2000, 2804, 2852
Bse1I ACTGG 2 cut(s) 1268, 2264
Bse21I CCTNAGG 3 cut(s) 852, 1848, 2700
Bse3DI GCAATG 1 cut(s) 70
BseBI CCWGG 8 cut(s) 252, 786, 957, 1782, 1953, 2634, 2805, 3117
BseDI CCNNGG 8 cut(s) 174, 375, 1020, 1243, 2016, 2239, 2868, 3091
BseGI GGATG 7 cut(s) 429, 732, 994, 1728, 1990, 2580, 2842
BseLI CCNNNNNNNGG 7 cut(s) 50, 956, 1004, 1952, 2000, 2804, 2852
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 9 cut(s) 329, 637, 750, 866, 1633, 1862, 2480, 2598, 2714
BseNI ACTGG 2 cut(s) 1268, 2264
BseXI GCAGC 8 cut(s) 164, 259, 584, 922, 1580, 1668, 1918, 2770
BseYI CCCAGC 1 cut(s) 401
BsgI GTGCAG 3 cut(s) 171, 1289, 2285
Bsh1236I CGCG 3 cut(s) 1050, 2046, 2898
BshNI GGYRCC 1 cut(s) 134
BsiSI CCGG 2 cut(s) 92, 138
BslFI GGGAC 3 cut(s) 630, 1626, 2478
BslI CCNNNNNNNGG 7 cut(s) 50, 956, 1004, 1952, 2000, 2804, 2852
BsmAI GTCTC 4 cut(s) 201, 714, 1710, 2562
BsmFI GGGAC 3 cut(s) 630, 1626, 2478
BsmI GAATGC 1 cut(s) 444
Bso31I GGTCTC 3 cut(s) 714, 1710, 2562
Bsp1407I TGTACA 6 cut(s) 870, 1170, 1866, 2166, 2718, 3018
BspACI CCGC 3 cut(s) 1048, 2044, 2896
BspCNI CTCAG 9 cut(s) 328, 636, 751, 865, 1632, 1861, 2481, 2599, 2713
BspFNI CGCG 3 cut(s) 1050, 2046, 2898
BspLI GGNNCC 7 cut(s) 136, 849, 858, 1845, 1854, 2697, 2706
BspMI ACCTGC 5 cut(s) 693, 1151, 2147, 2541, 2999
BspOI GCTAGC 3 cut(s) 828, 1824, 2676
BspT107I GGYRCC 1 cut(s) 134
BspTNI GGTCTC 3 cut(s) 714, 1710, 2562
BsrDI GCAATG 1 cut(s) 70
BsrGI TGTACA 6 cut(s) 870, 1170, 1866, 2166, 2718, 3018
BsrI ACTGG 2 cut(s) 1268, 2264
BssECI CCNNGG 8 cut(s) 174, 375, 1020, 1243, 2016, 2239, 2868, 3091
BssNI GRCGYC 2 cut(s) 120, 184
BssT1I CCWWGG 5 cut(s) 174, 375, 1243, 2239, 3091
Bst2UI CCWGG 8 cut(s) 252, 786, 957, 1782, 1953, 2634, 2805, 3117
Bst4CI ACNGT 7 cut(s) 389, 841, 1021, 1837, 2017, 2689, 2869
Bst6I CTCTTC 2 cut(s) 1281, 2277
BstACI GRCGYC 2 cut(s) 120, 184
BstAPI GCANNNNNTGC 1 cut(s) 158
BstAUI TGTACA 6 cut(s) 870, 1170, 1866, 2166, 2718, 3018
BstC8I GCNNGC 3 cut(s) 826, 1822, 2674
BstDSI CCRYGG 3 cut(s) 1020, 2016, 2868
BstENI CCTNNNNNAGG 3 cut(s) 1002, 1998, 2850
BstF5I GGATG 7 cut(s) 429, 732, 994, 1728, 1990, 2580, 2842
BstFNI CGCG 3 cut(s) 1050, 2046, 2898
BstH2I RGCGCY 2 cut(s) 1354, 2350
BstHHI GCGC 4 cut(s) 615, 1353, 1611, 2349
BstMAI GTCTC 4 cut(s) 201, 714, 1710, 2562
BstNI CCWGG 8 cut(s) 252, 786, 957, 1782, 1953, 2634, 2805, 3117
BstNSI RCATGY 8 cut(s) 710, 1227, 1387, 1706, 2223, 2383, 2558, 3075
BstSCI CCNGG 8 cut(s) 250, 784, 955, 1780, 1951, 2632, 2803, 3115
BstUI CGCG 3 cut(s) 1050, 2046, 2898
BstV1I GCAGC 8 cut(s) 164, 259, 584, 922, 1580, 1668, 1918, 2770
BstX2I RGATCY 3 cut(s) 911, 1907, 2759
BstYI RGATCY 3 cut(s) 911, 1907, 2759
Bsu36I CCTNAGG 3 cut(s) 852, 1848, 2700
BsuI GTATCC 1 cut(s) 349
BtgI CCRYGG 3 cut(s) 1020, 2016, 2868
BtsCI GGATG 7 cut(s) 429, 732, 994, 1728, 1990, 2580, 2842
BtsI GCAGTG 2 cut(s) 1301, 2297
BveI ACCTGC 5 cut(s) 693, 1151, 2147, 2541, 2999
Cac8I GCNNGC 3 cut(s) 826, 1822, 2674
CfoI GCGC 4 cut(s) 615, 1353, 1611, 2349
Cfr13I GGNCC 3 cut(s) 1265, 2261, 3113
CspCI CAANNNNNGTGG 4 cut(s) 1290, 1325, 2286, 2321
DraIII CACNNNGTG 2 cut(s) 626, 1622
DriI GACNNNNNGTC 1 cut(s) 188
Eam1104I CTCTTC 2 cut(s) 1281, 2277
Eam1105I GACNNNNNGTC 1 cut(s) 188
EarI CTCTTC 2 cut(s) 1281, 2277
Eco130I CCWWGG 5 cut(s) 174, 375, 1243, 2239, 3091
Eco31I GGTCTC 3 cut(s) 714, 1710, 2562
Eco47I GGWCC 3 cut(s) 1265, 2261, 3113
Eco57I CTGAAG 3 cut(s) 786, 1782, 2634
Eco81I CCTNAGG 3 cut(s) 852, 1848, 2700
EcoNI CCTNNNNNAGG 3 cut(s) 1002, 1998, 2850
EcoRII CCWGG 8 cut(s) 250, 784, 955, 1780, 1951, 2632, 2803, 3115
EcoT14I CCWWGG 5 cut(s) 174, 375, 1243, 2239, 3091
ErhI CCWWGG 5 cut(s) 174, 375, 1243, 2239, 3091
FalI AAGNNNNNCTT 2 cut(s) 1630, 1662
FaqI GGGAC 3 cut(s) 630, 1626, 2478
FauNDI CATATG 5 cut(s) 568, 1261, 1564, 2257, 3109
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 8 cut(s) 153, 248, 598, 936, 1594, 1682, 1932, 2784
FokI GGATG 7 cut(s) 416, 719, 981, 1715, 1977, 2567, 2829
Fsp4HI GCNGC 8 cut(s) 153, 248, 598, 936, 1594, 1682, 1932, 2784
FspBI CTAG 8 cut(s) 810, 825, 1398, 1806, 1821, 2394, 2658, 2673
FspI TGCGCA 2 cut(s) 614, 1610
GlaI GCGC 4 cut(s) 614, 1352, 1610, 2348
GluI GCNGC 8 cut(s) 153, 248, 598, 936, 1594, 1682, 1932, 2784
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 3 cut(s) 273, 570, 1566
HaeII RGCGCY 2 cut(s) 1354, 2350
HapII CCGG 2 cut(s) 92, 138
HhaI GCGC 4 cut(s) 615, 1353, 1611, 2349
Hin1I GRCGYC 2 cut(s) 120, 184
Hin6I GCGC 4 cut(s) 613, 1351, 1609, 2347
HinP1I GCGC 4 cut(s) 613, 1351, 1609, 2347
HpaII CCGG 2 cut(s) 92, 138
Hpy166II GTNNAC 2 cut(s) 1301, 2297
Hpy188III TCNNGA 5 cut(s) 284, 1408, 1432, 2404, 2428
Hpy8I GTNNAC 2 cut(s) 1301, 2297
Hpy99I CGWCG 3 cut(s) 122, 125, 189
HpyCH4III ACNGT 7 cut(s) 389, 841, 1021, 1837, 2017, 2689, 2869
HpyCH4IV ACGT 2 cut(s) 120, 184
HpySE526I ACGT 2 cut(s) 120, 184
Hsp92I GRCGYC 2 cut(s) 120, 184
HspAI GCGC 4 cut(s) 613, 1351, 1609, 2347
Ksp22I TGATCA 1 cut(s) 3
LmnI GCTCC 6 cut(s) 53, 577, 853, 1573, 1849, 2701
Lsp1109I GCAGC 8 cut(s) 164, 259, 584, 922, 1580, 1668, 1918, 2770
MaeI CTAG 8 cut(s) 810, 825, 1398, 1806, 1821, 2394, 2658, 2673
MaeII ACGT 2 cut(s) 120, 184
MaeIII GTNAC 5 cut(s) 454, 490, 680, 1486, 2528
MboII GAAGA 8 cut(s) 1034, 1046, 1268, 2030, 2042, 2264, 2882, 2894
MflI RGATCY 3 cut(s) 911, 1907, 2759
MlyI GAGTC 3 cut(s) 311, 652, 2500
MseI TTAA 9 cut(s) 47, 1001, 1290, 1355, 1997, 2286, 2351, 2849, 3154
MslI CAYNNNNRTG 3 cut(s) 705, 1701, 2553
MspA1I CMGCKG 3 cut(s) 868, 1864, 2716
MspI CCGG 2 cut(s) 92, 138
MspR9I CCNGG 8 cut(s) 252, 786, 957, 1782, 1953, 2634, 2805, 3117
Mva1269I GAATGC 1 cut(s) 444
MvaI CCWGG 8 cut(s) 252, 786, 957, 1782, 1953, 2634, 2805, 3117
MvnI CGCG 3 cut(s) 1050, 2046, 2898
NdeI CATATG 5 cut(s) 568, 1261, 1564, 2257, 3109
NheI GCTAGC 3 cut(s) 824, 1820, 2672
NlaIV GGNNCC 7 cut(s) 136, 849, 858, 1845, 1854, 2697, 2706
NmuCI GTSAC 4 cut(s) 490, 680, 1486, 2528
NsbI TGCGCA 2 cut(s) 614, 1610
NspI RCATGY 8 cut(s) 710, 1227, 1387, 1706, 2223, 2383, 2558, 3075
PaqCI CACCTGC 3 cut(s) 1151, 2147, 2999
PciI ACATGT 3 cut(s) 706, 1702, 2554
PctI GAATGC 1 cut(s) 444
PfeI GAWTC 9 cut(s) 763, 977, 1109, 1759, 1973, 2105, 2611, 2825, 2957
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 8 cut(s) 154, 249, 599, 937, 1595, 1683, 1933, 2785
PleI GAGTC 3 cut(s) 310, 651, 2499
PpsI GAGTC 3 cut(s) 310, 651, 2499
PscI ACATGT 3 cut(s) 706, 1702, 2554
PsiI TTATAA 2 cut(s) 1439, 2435
Psp6I CCWGG 8 cut(s) 250, 784, 955, 1780, 1951, 2632, 2803, 3115
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 8 cut(s) 250, 784, 955, 1780, 1951, 2632, 2803, 3115
PspN4I GGNNCC 7 cut(s) 136, 849, 858, 1845, 1854, 2697, 2706
PspPI GGNCC 3 cut(s) 1265, 2261, 3113
PsuI RGATCY 3 cut(s) 911, 1907, 2759
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 3 cut(s) 868, 1864, 2716
RseI CAYNNNNRTG 3 cut(s) 705, 1701, 2553
SaqAI TTAA 9 cut(s) 47, 1001, 1290, 1355, 1997, 2286, 2351, 2849, 3154
SatI GCNGC 8 cut(s) 153, 248, 598, 936, 1594, 1682, 1932, 2784
Sau96I GGNCC 3 cut(s) 1265, 2261, 3113
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 3 cut(s) 311, 652, 2500
ScrFI CCNGG 8 cut(s) 252, 786, 957, 1782, 1953, 2634, 2805, 3117
SinI GGWCC 3 cut(s) 1265, 2261, 3113
SmiMI CAYNNNNRTG 3 cut(s) 705, 1701, 2553
SmlI CTYRAG 4 cut(s) 263, 284, 1408, 2404
SmoI CTYRAG 4 cut(s) 263, 284, 1408, 2404
SsiI CCGC 3 cut(s) 1048, 2044, 2896
SspMI CTAG 8 cut(s) 810, 825, 1398, 1806, 1821, 2394, 2658, 2673
StyD4I CCNGG 8 cut(s) 250, 784, 955, 1780, 1951, 2632, 2803, 3115
StyI CCWWGG 5 cut(s) 174, 375, 1243, 2239, 3091
TaaI ACNGT 7 cut(s) 389, 841, 1021, 1837, 2017, 2689, 2869
TaiI ACGT 2 cut(s) 123, 187
TaqI TCGA 5 cut(s) 504, 777, 1500, 1773, 2625
TaqII GACCGA 1 cut(s) 230
TatI WGTACW 7 cut(s) 11, 870, 1170, 1866, 2166, 2718, 3018
TfiI GAWTC 9 cut(s) 763, 977, 1109, 1759, 1973, 2105, 2611, 2825, 2957
Tru1I TTAA 9 cut(s) 47, 1001, 1290, 1355, 1997, 2286, 2351, 2849, 3154
Tru9I TTAA 9 cut(s) 47, 1001, 1290, 1355, 1997, 2286, 2351, 2849, 3154
TseFI GTSAC 4 cut(s) 490, 680, 1486, 2528
TseI GCWGC 8 cut(s) 152, 247, 597, 935, 1593, 1681, 1931, 2783
Tsp45I GTSAC 4 cut(s) 490, 680, 1486, 2528
TspDTI ATGAA 7 cut(s) 418, 969, 1248, 1965, 2244, 2817, 3096
Tth111I GACNNNGTC 1 cut(s) 122
VpaK11BI GGWCC 3 cut(s) 1265, 2261, 3113
XagI CCTNNNNNAGG 3 cut(s) 1002, 1998, 2850
XceI RCATGY 8 cut(s) 710, 1227, 1387, 1706, 2223, 2383, 2558, 3075
XcmI CCANNNNNNNNNTGG 3 cut(s) 1153, 2149, 3001
XspI CTAG 8 cut(s) 810, 825, 1398, 1806, 1821, 2394, 2658, 2673
ZraI GACGTC 2 cut(s) 121, 185
ZrmI AGTACT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.