MD00G1080800.v1.1

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000108
Physical Location & Seq
Reverse (-)
16132953 .. 16136424
3472 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1080800.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGATTACCAGTATGTTCTTGGTGGGGTGTTGGTTTCTCTGCTGGGCTCTGTTTTCTTCATGATCATCAACACCACTCTCAGCGGCGAGAAGAAGAAGGTGGTCGAGAAAGTTTCCAACGGCGTTAACGGAAATGGTTACGTGAGGATGCCGACCCAGAACGGTAATTTTCAACCGGAGAGTGCCAGTGGCACGGACGTTGTCATTGTCGGTGCCGGAGTTGCCGGTGCTGCCCTTGCTTACACTCTTGCGAAGGACGGACGCCGGGTACATGTGATCGAAAGAGACTTGAGTGAGCCAGACAGAATTGTTGGTGAACTATTGCAGCCTGGAGGCTATCTCAAATTGATTGAGTTGGGTCTTGAAGACTGTGCAAATGAGTCCATTGACGCTCAAAAAGTATTTGGTTATGCTCTCTACAAAGATGGGAACGACACAAAACTGTCTTATCCCTTGGAAAACTATCCTTCAGATATCGCCGGAAGAAGTTTCCACAACGGGCGTTTCATCCAAAAAATGCGTGAAAGGGCTACAGCTCTTAAAAACGTGACACTAGAACAAGGAACTGTGACAACCCTAATTGAAGAAAAGGGCACCGTCAAGGGTGTGATGTACAAGAACAAGGCTGGAGACGAGATGAGATCATATGCTCCATTAACAATAGTATGCGATGGGTGCTTTTCAAATCTCCGCCGCAATCTCTGTACTCCAAAGGTTGACAATCCTTCTTGCTTCGTCGGTTTGATCTTGGAGAACTGTGACCTTCCTTATGCAAACCACGGACACGTGATTTTGGGAGACCCTTCACCTATCTTATTTTACCCTATTAGTGGTACCGAAGTTCGTTGTTTGGTAGATGTTCCCGGCACAAAAGTACCTTCAGTAGCTAATGGTGAAATGGCCAAATACTTGAAAAATGTGGTTGCTCCTCAGGTTCCCCCTCAGCTCTTGAGATCTTTTCTCGCAGCAGTTGAGAAAGGAAACATCAGAACAATGCAAAACAAAAGCATGCCCGCTACTCCTCAACCCACTCCCGGTGCAATTTTATTAGGGGATGCATTCAATATGAGGCATCCTTTGACCGGAGGAGGAATGACCGTGGCTCTTTCCGACATTGTTCTTCTAAGGGATCTTCTTAGACCCCTAACTGATCTCAACGATGCACCAGCTTTGTGCGAATATCTAGAATCATTCTACACACTTCGCAAGCCTGTGTCATCTACCATAAACACATTGGCCGGTGCTTTGTACAAGGTGTTTTGTGCATCACCTGACCCGGCAAGACAGGAAATGAGGGAAGCTTGTTTCGATTATTTGAGCCTCGGAGGCATATGTGCAAGGGGACCAATATCTCTACTCTCTGGCCTTAATCCTCGTCCGATCCACCTTTTTCTCCATTTCTTTGCCGTGGCTGTCTATGGAGTAGGTCGCTTAATGATTCCATTCCCTACGCCTAAACGTGTATGGCTCGGGACCAGATTGATCTTGGGTGCATCAGGAATTATATTCCCCATTATTAAAGGTGAAGGAGTTAGACAAATGTTCTTTCCTGCAACAATCCCAGCATATTACAGAGCTCCTCCTCTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

531

Amino Acids

57.64

Weight (kDa)

8.61

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 65 - 376 1.7e-17 FAD binding domain
DAO PF01266 66 - 96 7.9e-08 FAD dependent oxidoreductase
NAD_binding_8 PF13450 69 - 95 2.7e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 217 - 489 9.3e-120 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 833
AccB1I GGYRCC 3 cut(s) 212, 593, 833
AciI CCGC 4 cut(s) 84, 691, 694, 1014
AclWI GGATC 2 cut(s) 1137, 1375
AcoI YGGCCR 2 cut(s) 900, 1236
AcuI CTGAAG 2 cut(s) 453, 864
AcvI CACGTG 1 cut(s) 787
AcyI GRCGYC 1 cut(s) 262
AfaI GTAC 6 cut(s) 270, 614, 706, 835, 876, 1250
AfiI CCNNNNNNNGG 3 cut(s) 830, 1034, 1082
AflIII ACRYGT 3 cut(s) 271, 784, 1459
AgsI TTSAA 7 cut(s) 173, 365, 584, 684, 913, 1063, 1589
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 6 cut(s) 536, 887, 946, 1169, 1301, 1577
AluI AGCT 6 cut(s) 536, 887, 946, 1169, 1301, 1577
Alw21I GWGCWC 1 cut(s) 1579
Alw26I GTCTC 3 cut(s) 279, 624, 792
AlwI GGATC 2 cut(s) 1137, 1375
Ama87I CYCGRG 1 cut(s) 1469
AoxI GGCC 3 cut(s) 900, 1236, 1363
ApeKI GCWGC 3 cut(s) 230, 325, 965
Asp718I GGTACC 1 cut(s) 833
AspS9I GGNCC 2 cut(s) 1343, 1473
AsuC2I CCSGG 4 cut(s) 266, 864, 1035, 1277
AsuHPI GGTGA 5 cut(s) 326, 798, 905, 1260, 1535
AvaI CYCGRG 1 cut(s) 1469
AvaII GGWCC 2 cut(s) 1343, 1473
AxyI CCTNAGG 1 cut(s) 930
BaeGI GKGCMC 1 cut(s) 596
BaeI ACNNNNGTAYC 4 cut(s) 825, 858, 858, 891
BalI TGGCCA 1 cut(s) 902
BanI GGYRCC 3 cut(s) 212, 593, 833
BanII GRGCYC 2 cut(s) 50, 1579
BbrPI CACGTG 1 cut(s) 787
BbsI GAAGAC 1 cut(s) 372
Bbv12I GWGCWC 1 cut(s) 1579
BbvCI CCTCAGC 1 cut(s) 942
BbvI GCAGC 3 cut(s) 217, 337, 977
BccI CCATC 2 cut(s) 419, 665
BceAI ACGGC 2 cut(s) 136, 1391
BciT130I CCWGG 1 cut(s) 330
BclI TGATCA 1 cut(s) 63
BcnI CCSGG 4 cut(s) 266, 864, 1035, 1277
BcoDI GTCTC 3 cut(s) 279, 624, 792
BfaI CTAG 2 cut(s) 554, 1184
BfmI CTRYAG 1 cut(s) 531
BglI GCCNNNNNGGC 1 cut(s) 1326
BglII AGATCT 1 cut(s) 953
BisI GCNGC 5 cut(s) 85, 231, 326, 694, 966
BlsI GCNGC 5 cut(s) 86, 232, 327, 695, 967
Bme1390I CCNGG 5 cut(s) 266, 330, 864, 1035, 1277
Bme18I GGWCC 2 cut(s) 1343, 1473
BmeT110I CYCGRG 1 cut(s) 1469
BmgT120I GGNCC 2 cut(s) 1343, 1473
BmiI GGNNCC 6 cut(s) 214, 595, 835, 936, 1344, 1474
BmrFI CCNGG 5 cut(s) 266, 330, 864, 1035, 1277
BmsI GCATC 6 cut(s) 138, 1045, 1081, 1150, 1274, 1502
BpiI GAAGAC 1 cut(s) 372
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BpmI CTGGAG 2 cut(s) 351, 648
Bpu10I CCTNAGC 1 cut(s) 942
BpuEI CTTGAG 2 cut(s) 310, 970
BpuMI CCSGG 4 cut(s) 266, 864, 1035, 1277
BsaAI YACGTR 2 cut(s) 142, 787
BsaHI GRCGYC 1 cut(s) 262
BsaI GGTCTC 1 cut(s) 792
BsaJI CCNNGG 5 cut(s) 453, 778, 1098, 1321, 1407
BsaWI WCCGGW 2 cut(s) 175, 1082
Bsc4I CCNNNNNNNGG 3 cut(s) 830, 1034, 1082
Bse118I RCCGGY 2 cut(s) 224, 1238
Bse1I ACTGG 2 cut(s) 10, 186
Bse21I CCTNAGG 1 cut(s) 930
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 5 cut(s) 453, 778, 1098, 1321, 1407
BseGI GGATG 4 cut(s) 153, 507, 1060, 1072
BseLI CCNNNNNNNGG 3 cut(s) 830, 1034, 1082
BseMII CTCAG 3 cut(s) 94, 944, 956
BseNI ACTGG 2 cut(s) 10, 186
BseRI GAGGAG 5 cut(s) 918, 1011, 1101, 1569, 1572
BseSI GKGCMC 1 cut(s) 596
BseXI GCAGC 3 cut(s) 217, 337, 977
BseYI CCCAGC 2 cut(s) 43, 1561
BshFI GGCC 3 cut(s) 902, 1238, 1365
BshNI GGYRCC 3 cut(s) 212, 593, 833
BsiHKAI GWGCWC 1 cut(s) 1579
BsiHKCI CYCGRG 1 cut(s) 1469
BslFI GGGAC 2 cut(s) 1356, 1486
BslI CCNNNNNNNGG 3 cut(s) 830, 1034, 1082
BsmAI GTCTC 3 cut(s) 279, 624, 792
BsmBI CGTCTC 1 cut(s) 624
BsmFI GGGAC 2 cut(s) 1356, 1486
BsmI GAATGC 1 cut(s) 1058
BsnI GGCC 3 cut(s) 902, 1238, 1365
Bso31I GGTCTC 1 cut(s) 792
BsoBI CYCGRG 1 cut(s) 1469
Bsp1286I GDGCHC 3 cut(s) 50, 596, 1579
Bsp1407I TGTACA 2 cut(s) 612, 1248
Bsp143I GATC 9 cut(s) 63, 276, 641, 744, 953, 1129, 1150, 1380, 1482
BspACI CCGC 4 cut(s) 84, 691, 694, 1014
BspANI GGCC 3 cut(s) 902, 1238, 1365
BspCNI CTCAG 3 cut(s) 93, 943, 955
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 6 cut(s) 214, 595, 835, 936, 1344, 1474
BspPI GGATC 2 cut(s) 1137, 1375
BspT107I GGYRCC 3 cut(s) 212, 593, 833
BspTNI GGTCTC 1 cut(s) 792
BsrFI RCCGGY 2 cut(s) 224, 1238
BsrGI TGTACA 2 cut(s) 612, 1248
BsrI ACTGG 2 cut(s) 10, 186
BssAI RCCGGY 2 cut(s) 224, 1238
BssECI CCNNGG 5 cut(s) 453, 778, 1098, 1321, 1407
BssMI GATC 9 cut(s) 63, 276, 641, 744, 953, 1129, 1150, 1380, 1482
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 7 cut(s) 164, 371, 444, 568, 598, 758, 1099
Bst6I CTCTTC 1 cut(s) 1590
BstACI GRCGYC 1 cut(s) 262
BstAUI TGTACA 2 cut(s) 612, 1248
BstBAI YACGTR 2 cut(s) 142, 787
BstC8I GCNNGC 3 cut(s) 1010, 1014, 1208
BstDEI CTNAG 5 cut(s) 80, 930, 942, 1124, 1136
BstDSI CCRYGG 3 cut(s) 778, 1098, 1407
BstF5I GGATG 4 cut(s) 153, 507, 1060, 1072
BstKTI GATC 9 cut(s) 66, 279, 644, 747, 956, 1132, 1153, 1383, 1485
BstMAI GTCTC 3 cut(s) 279, 624, 792
BstMBI GATC 9 cut(s) 63, 276, 641, 744, 953, 1129, 1150, 1380, 1482
BstMWI GCNNNNNNNGC 5 cut(s) 221, 230, 236, 675, 1326
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 2 cut(s) 275, 1012
BstSCI CCNGG 5 cut(s) 264, 328, 862, 1033, 1275
BstSFI CTRYAG 1 cut(s) 531
BstSLI GKGCMC 1 cut(s) 596
BstV1I GCAGC 3 cut(s) 217, 337, 977
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 2 cut(s) 953, 1129
BstYI RGATCY 2 cut(s) 953, 1129
Bsu36I CCTNAGG 1 cut(s) 930
BsuRI GGCC 3 cut(s) 902, 1238, 1365
BtgI CCRYGG 3 cut(s) 778, 1098, 1407
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 4 cut(s) 153, 507, 1060, 1072
BtsIMutI CAGTG 1 cut(s) 193
Cac8I GCNNGC 3 cut(s) 1010, 1014, 1208
CciI TCATGA 1 cut(s) 60
Cfr10I RCCGGY 2 cut(s) 224, 1238
Cfr13I GGNCC 2 cut(s) 1343, 1473
CseI GACGC 2 cut(s) 270, 398
Csp6I GTAC 6 cut(s) 269, 613, 705, 834, 875, 1249
CviAII CATG 3 cut(s) 61, 272, 1009
CviQI GTAC 6 cut(s) 269, 613, 705, 834, 875, 1249
DdeI CTNAG 5 cut(s) 80, 930, 942, 1124, 1136
DpnI GATC 9 cut(s) 65, 278, 643, 746, 955, 1131, 1152, 1382, 1484
DpnII GATC 9 cut(s) 63, 276, 641, 744, 953, 1129, 1150, 1380, 1482
EaeI YGGCCR 2 cut(s) 900, 1236
Eam1104I CTCTTC 1 cut(s) 1590
EarI CTCTTC 1 cut(s) 1590
EciI GGCGGA 1 cut(s) 680
Ecl136II GAGCTC 1 cut(s) 1577
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 2 cut(s) 50, 1579
Eco31I GGTCTC 1 cut(s) 792
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 1343, 1473
Eco53kI GAGCTC 1 cut(s) 1577
Eco57I CTGAAG 2 cut(s) 453, 864
Eco72I CACGTG 1 cut(s) 787
Eco81I CCTNAGG 1 cut(s) 930
Eco88I CYCGRG 1 cut(s) 1469
EcoICRI GAGCTC 1 cut(s) 1577
EcoRII CCWGG 1 cut(s) 328
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 1 cut(s) 453
EcoT22I ATGCAT 1 cut(s) 1060
EcoT38I GRGCYC 2 cut(s) 50, 1579
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 624
FaeI CATG 3 cut(s) 64, 275, 1012
FaqI GGGAC 2 cut(s) 1356, 1486
FatI CATG 3 cut(s) 60, 271, 1008
FauI CCCGC 1 cut(s) 1021
FauNDI CATATG 2 cut(s) 646, 1331
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 5 cut(s) 85, 231, 326, 694, 966
FokI GGATG 4 cut(s) 160, 494, 1059, 1067
FriOI GRGCYC 2 cut(s) 50, 1579
Fsp4HI GCNGC 5 cut(s) 85, 231, 326, 694, 966
FspBI CTAG 2 cut(s) 554, 1184
GluI GCNGC 5 cut(s) 85, 231, 326, 694, 966
GsaI CCCAGC 2 cut(s) 47, 1565
GsuI CTGGAG 2 cut(s) 351, 648
HaeIII GGCC 3 cut(s) 902, 1238, 1365
HgaI GACGC 2 cut(s) 270, 398
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 3 cut(s) 64, 275, 1012
HincII GTYRAC 2 cut(s) 127, 718
HindII GTYRAC 2 cut(s) 127, 718
HindIII AAGCTT 1 cut(s) 1299
HinfI GANTC 3 cut(s) 380, 1187, 1438
HpaI GTTAAC 1 cut(s) 127
HphI GGTGA 5 cut(s) 326, 798, 905, 1260, 1535
Hpy166II GTNNAC 3 cut(s) 127, 317, 718
Hpy188I TCNGA 5 cut(s) 472, 989, 1111, 1325, 1380
Hpy188III TCNNGA 7 cut(s) 61, 106, 362, 949, 1184, 1471, 1497
Hpy8I GTNNAC 3 cut(s) 127, 317, 718
Hpy99I CGWCG 1 cut(s) 740
HpyAV CCTTC 8 cut(s) 91, 247, 477, 735, 773, 813, 888, 1520
HpyCH4III ACNGT 7 cut(s) 164, 371, 444, 568, 598, 758, 1099
HpyCH4IV ACGT 5 cut(s) 141, 198, 546, 786, 1459
HpyF10VI GCNNNNNNNGC 5 cut(s) 221, 230, 236, 675, 1326
HpyF3I CTNAG 5 cut(s) 80, 930, 942, 1124, 1136
HpySE526I ACGT 5 cut(s) 141, 198, 546, 786, 1459
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 3 cut(s) 64, 275, 1012
KpnI GGTACC 1 cut(s) 837
Ksp22I TGATCA 1 cut(s) 63
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 9 cut(s) 63, 276, 641, 744, 953, 1129, 1150, 1380, 1482
LmnI GCTCC 3 cut(s) 655, 931, 1582
Lsp1109I GCAGC 3 cut(s) 217, 337, 977
LweI GCATC 6 cut(s) 138, 1045, 1081, 1150, 1274, 1502
MaeI CTAG 2 cut(s) 554, 1184
MaeII ACGT 5 cut(s) 141, 198, 546, 786, 1459
MaeIII GTNAC 4 cut(s) 137, 547, 568, 758
MalI GATC 9 cut(s) 65, 278, 643, 746, 955, 1131, 1152, 1382, 1484
MboI GATC 9 cut(s) 63, 276, 641, 744, 953, 1129, 1150, 1380, 1482
MboII GAAGA 9 cut(s) 49, 103, 106, 377, 495, 596, 1112, 1124, 1577
MflI RGATCY 2 cut(s) 953, 1129
MhlI GDGCHC 3 cut(s) 50, 596, 1579
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 6 cut(s) 166, 306, 344, 579, 1041, 1500
MluNI TGGCCA 1 cut(s) 902
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 2 cut(s) 141, 1134
Mox20I TGGCCA 1 cut(s) 902
Mph1103I ATGCAT 1 cut(s) 1060
MscI TGGCCA 1 cut(s) 902
MseI TTAA 6 cut(s) 126, 540, 656, 1368, 1433, 1518
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 1 cut(s) 84
MspR9I CCNGG 5 cut(s) 266, 330, 864, 1035, 1277
Mva1269I GAATGC 1 cut(s) 1058
MvaI CCWGG 1 cut(s) 330
MwoI GCNNNNNNNGC 5 cut(s) 221, 230, 236, 675, 1326
NciI CCSGG 4 cut(s) 266, 864, 1035, 1277
NdeI CATATG 2 cut(s) 646, 1331
NdeII GATC 9 cut(s) 63, 276, 641, 744, 953, 1129, 1150, 1380, 1482
NlaIII CATG 3 cut(s) 64, 275, 1012
NlaIV GGNNCC 6 cut(s) 214, 595, 835, 936, 1344, 1474
NmuCI GTSAC 3 cut(s) 547, 568, 758
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 2 cut(s) 275, 1012
PaeI GCATGC 1 cut(s) 1012
PagI TCATGA 1 cut(s) 60
PciI ACATGT 1 cut(s) 271
PctI GAATGC 1 cut(s) 1058
PfeI GAWTC 2 cut(s) 1187, 1438
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 5 cut(s) 86, 232, 327, 695, 967
PleI GAGTC 1 cut(s) 388
PmaCI CACGTG 1 cut(s) 787
PmlI CACGTG 1 cut(s) 787
PpsI GAGTC 1 cut(s) 388
Ppu21I YACGTR 2 cut(s) 142, 787
PscI ACATGT 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 1579
Psp6I CCWGG 1 cut(s) 328
PspCI CACGTG 1 cut(s) 787
PspFI CCCAGC 2 cut(s) 43, 1561
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 6 cut(s) 214, 595, 835, 936, 1344, 1474
PspPI GGNCC 2 cut(s) 1343, 1473
PsuI RGATCY 2 cut(s) 953, 1129
PsyI GACNNNGTC 1 cut(s) 200
RsaI GTAC 6 cut(s) 270, 614, 706, 835, 876, 1250
RsaNI GTAC 6 cut(s) 269, 613, 705, 834, 875, 1249
SacI GAGCTC 1 cut(s) 1579
SaqAI TTAA 6 cut(s) 126, 540, 656, 1368, 1433, 1518
SatI GCNGC 5 cut(s) 85, 231, 326, 694, 966
Sau3AI GATC 9 cut(s) 63, 276, 641, 744, 953, 1129, 1150, 1380, 1482
Sau96I GGNCC 2 cut(s) 1343, 1473
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 5 cut(s) 266, 330, 864, 1035, 1277
SduI GDGCHC 3 cut(s) 50, 596, 1579
SfaNI GCATC 6 cut(s) 138, 1045, 1081, 1150, 1274, 1502
SfcI CTRYAG 1 cut(s) 531
SinI GGWCC 2 cut(s) 1343, 1473
SmlI CTYRAG 2 cut(s) 289, 949
SmoI CTYRAG 2 cut(s) 289, 949
SphI GCATGC 1 cut(s) 1012
Sse9I AATT 6 cut(s) 166, 306, 344, 579, 1041, 1500
SsiI CCGC 4 cut(s) 84, 691, 694, 1014
SspMI CTAG 2 cut(s) 554, 1184
SstI GAGCTC 1 cut(s) 1579
StyD4I CCNGG 5 cut(s) 264, 328, 862, 1033, 1275
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 7 cut(s) 164, 371, 444, 568, 598, 758, 1099
TaiI ACGT 5 cut(s) 144, 201, 549, 789, 1462
TaqI TCGA 3 cut(s) 105, 279, 1308
TasI AATT 6 cut(s) 166, 306, 344, 579, 1041, 1500
TatI WGTACW 3 cut(s) 612, 704, 1248
TauI GCSGC 2 cut(s) 87, 696
TfiI GAWTC 2 cut(s) 1187, 1438
Tru1I TTAA 6 cut(s) 126, 540, 656, 1368, 1433, 1518
Tru9I TTAA 6 cut(s) 126, 540, 656, 1368, 1433, 1518
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 3 cut(s) 547, 568, 758
TseI GCWGC 3 cut(s) 230, 325, 965
Tsp45I GTSAC 3 cut(s) 547, 568, 758
TspDTI ATGAA 2 cut(s) 49, 496
TspGWI ACGGA 4 cut(s) 144, 209, 273, 795
TspRI CASTG 1 cut(s) 193
Tth111I GACNNNGTC 1 cut(s) 200
VpaK11BI GGWCC 2 cut(s) 1343, 1473
XbaI TCTAGA 1 cut(s) 1183
XceI RCATGY 2 cut(s) 275, 1012
XcmI CCANNNNNNNNNTGG 3 cut(s) 17, 1231, 1483
XspI CTAG 2 cut(s) 554, 1184
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.