Rw4G018850

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000108
Physical Location & Seq
Reverse (-)
41687490 .. 41690113
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G018850.1

Sequence Viewer

Length: 1581 bp
ATGGTGTCTTACGAGTATATTTTGGGTGGGGTCTTGGTTTCATTGCTGGGCTCTATTTTCTTCATGATCATCAGCACTACTTTGGGTGGAAAGAAACCATGCAAGGCTTCAAATGGAGGTGTAAAAAGTAACGATGGGTTTGGGAACAGTGCTATTCAGCCGGAAATGGAGATGAAAAGTACCGATGTCGTCATTGTCGGTGCCGGAGTTGCCGGTGCAGCTCTTGCTTACACTCTTGCCAAGGAAGGTCGTCGTGTACATGTCATCGAAAGAGACTTGACTGAGCCAGACAGAATTGTTGGTGAGCTGTTGCAGCCTGGGGGTTATCTCAAGTTGATTGAGTTGGGTCTCGAGGATTGTGCAAATGAGTCAATTGATGCTCAGAAGGTGTTTGGCTATGCTCTTTACAAAGATGGCAAGGATACCAAACTGACTTATCCCTTAGAGAAATACAGTACAGATGTGGCTGGGAGAAGTTTCCACAATGGGCGTTTCATCCAAAGAATGCGCGAAAAAGCTATGACTCTTTCAAATGTAAAATTGGAACAAGGATCAGTGACAACATTAATCGAGGAAAAGGGCATTGTCAAAGGGGTGATTTACAAGAACAAGGCTGGAGAGGAGATGAGAACATATGCTCCACTAACAATCGTGTGTGATGGTTGCTTTTCAAATCTGCGCAAGTCTCTCAGTGCACCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGATCTTGGAGAACTGTGACCTTCCACACGCAAATCATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTCTTTTATCCTATCAGTAGCACCGAGGTTCGCTGTTTGGTGGATGTACCTGGAACAAAAGTACCTTCAGTAGCTAGTGGTGAAATGGCTAACTATTTGAAAACCGTGGTGGCTCCTCAGATTCCCCCACAGCTGCACAATGCTTTTATGGCCGCAGTAGAGAAGGGAAACATCAGAACAATGCAAAACAAAAGCATGGCTGCTAATCCTGTCCCTACTCCTGGTGCAATTTTATTAGGGGATGCATTCAACATGAGGCACCCTTTAACAGGAGGAGGAATGACCGTTGCTCTTTCAGACATTGTTCTTCTCCGTGATCTTCTTAGACCCCTACATGATCTCAATGATGCACCTGCTTTGTGCAATTACCTTGAATCATTCTACACACTGCGCAAGCCTGTATCATCTACCATAAACACATTGGCAGGTGCTTTGTACAAAGTGTTTTGTGCATCACCGGATCCAGCAAGACAGGAAATGCGTGAAGCATGTTTTGGCTATTTGAGCTTTGGAGGCATCTGTTCATACGGACCAGTATCTCTTCTCTCTGGTCTTAACCCTCAGCCACTGCACTTGTTTCTCCATTTCTTTGCTGTTGCCATCTATGGTGTCGGCCGCTTAATGCTTCCATTCCCTTCACCTAAACGCATGTGGCTTGGGATTCGATTGATCTTGAGTGCAGCTGGCATCATATTCCCCATTATAAAAGGTGAAGGAGTTAGACAGATGTTCTTTCCTGCCACAGTACCAGCATATTACAGATCTCCTCCTGTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

526

Amino Acids

57.01

Weight (kDa)

8.61

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 61 - 372 6.7e-20 FAD binding domain
DAO PF01266 62 - 92 6.2e-08 FAD dependent oxidoreductase
NAD_binding_8 PF13450 65 - 91 2.3e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 213 - 485 7.5e-118 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1505
AarI CACCTGC 2 cut(s) 1162, 1217
Acc16I TGCGCA 2 cut(s) 680, 1193
Acc36I ACCTGC 2 cut(s) 1162, 1217
AccB1I GGYRCC 2 cut(s) 200, 1059
AccII CGCG 1 cut(s) 510
AciI CCGC 2 cut(s) 954, 1417
AclWI GGATC 3 cut(s) 559, 1256, 1269
AcoI YGGCCR 2 cut(s) 951, 1414
AcuI CTGAAG 1 cut(s) 852
AfaI GTAC 7 cut(s) 181, 258, 457, 849, 864, 1238, 1548
AfiI CCNNNNNNNGG 2 cut(s) 1022, 1070
AflIII ACRYGT 2 cut(s) 259, 772
AgsI TTSAA 7 cut(s) 111, 531, 672, 707, 901, 1051, 1175
AjnI CCWGG 3 cut(s) 316, 850, 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 1278, 1310
AloI GAACNNNNNNTCC 2 cut(s) 622, 654
AluBI AGCT 7 cut(s) 221, 307, 518, 875, 934, 1308, 1484
AluI AGCT 7 cut(s) 221, 307, 518, 875, 934, 1308, 1484
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 4 cut(s) 267, 353, 690, 780
Alw44I GTGCAC 1 cut(s) 693
AlwI GGATC 3 cut(s) 559, 1256, 1269
AlwNI CAGNNNCTG 1 cut(s) 1369
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 2 cut(s) 951, 1414
ApaLI GTGCAC 1 cut(s) 693
ApeKI GCWGC 5 cut(s) 218, 313, 934, 1001, 1481
AseI ATTAAT 1 cut(s) 566
AspLEI GCGC 3 cut(s) 510, 681, 1194
AspS9I GGNCC 1 cut(s) 1331
AsuHPI GGTGA 7 cut(s) 314, 607, 786, 893, 1248, 1431, 1523
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 1 cut(s) 1331
BaeGI GKGCMC 1 cut(s) 697
BaeI ACNNNNGTAYC 2 cut(s) 846, 879
BamHI GGATCC 1 cut(s) 1261
BanI GGYRCC 2 cut(s) 200, 1059
BanII GRGCYC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 697
BbvCI CCTCAGC 1 cut(s) 1362
BbvI GCAGC 5 cut(s) 230, 325, 921, 988, 1493
BccI CCATC 5 cut(s) 128, 407, 653, 806, 1409
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 3 cut(s) 318, 852, 1023
BciVI GTATCC 1 cut(s) 415
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 4 cut(s) 267, 353, 690, 780
BfaI CTAG 1 cut(s) 876
BfuAI ACCTGC 2 cut(s) 1162, 1217
BfuI GTATCC 1 cut(s) 415
BglII AGATCT 1 cut(s) 1562
BisI GCNGC 7 cut(s) 219, 314, 935, 954, 1002, 1417, 1482
BlsI GCNGC 7 cut(s) 220, 315, 936, 955, 1003, 1418, 1483
Bme1390I CCNGG 3 cut(s) 318, 852, 1023
Bme18I GGWCC 1 cut(s) 1331
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 1331
BmiI GGNNCC 4 cut(s) 202, 915, 1061, 1263
BmrFI CCNGG 3 cut(s) 318, 852, 1023
BmsI GCATC 6 cut(s) 367, 1033, 1138, 1262, 1326, 1497
BpmI CTGGAG 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 1362
BpuEI CTTGAG 2 cut(s) 314, 1495
BsaI GGTCTC 2 cut(s) 353, 780
BsaJI CCNNGG 4 cut(s) 240, 317, 825, 906
BsaWI WCCGGW 1 cut(s) 1258
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 2 cut(s) 1022, 1070
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 1334
Bse3DI GCAATG 1 cut(s) 41
BseBI CCWGG 3 cut(s) 318, 852, 1023
BseDI CCNNGG 4 cut(s) 240, 317, 825, 906
BseGI GGATG 4 cut(s) 495, 798, 850, 1048
BseLI CCNNNNNNNGG 2 cut(s) 1022, 1070
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 5 cut(s) 273, 395, 703, 932, 1376
BseNI ACTGG 1 cut(s) 1334
BseRI GAGGAG 4 cut(s) 635, 906, 1089, 1557
BseSI GKGCMC 1 cut(s) 697
BseX3I CGGCCG 1 cut(s) 1414
BseXI GCAGC 5 cut(s) 230, 325, 921, 988, 1493
BseYI CCCAGC 2 cut(s) 46, 467
BsgI GTGCAG 4 cut(s) 237, 920, 1355, 1500
Bsh1236I CGCG 1 cut(s) 510
Bsh1285I CGRYCG 1 cut(s) 1417
BshFI GGCC 2 cut(s) 953, 1416
BshNI GGYRCC 2 cut(s) 200, 1059
BsiEI CGRYCG 1 cut(s) 1417
BsiHKAI GWGCWC 1 cut(s) 697
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 4 cut(s) 161, 204, 213, 1259
BslFI GGGAC 2 cut(s) 696, 998
BslI CCNNNNNNNGG 2 cut(s) 1022, 1070
BsmAI GTCTC 4 cut(s) 267, 353, 690, 780
BsmFI GGGAC 2 cut(s) 696, 998
BsmI GAATGC 2 cut(s) 510, 1046
BsnI GGCC 2 cut(s) 953, 1416
Bso31I GGTCTC 2 cut(s) 353, 780
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 53, 697
Bsp1407I TGTACA 2 cut(s) 256, 1236
Bsp143I GATC 8 cut(s) 66, 551, 732, 1117, 1138, 1261, 1470, 1562
BspACI CCGC 2 cut(s) 954, 1417
BspANI GGCC 2 cut(s) 953, 1416
BspCNI CTCAG 5 cut(s) 274, 394, 702, 931, 1375
BspFNI CGCG 1 cut(s) 510
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 4 cut(s) 202, 915, 1061, 1263
BspMI ACCTGC 2 cut(s) 1162, 1217
BspPI GGATC 3 cut(s) 559, 1256, 1269
BspT107I GGYRCC 2 cut(s) 200, 1059
BspTNI GGTCTC 2 cut(s) 353, 780
BsrDI GCAATG 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 212
BsrGI TGTACA 2 cut(s) 256, 1236
BsrI ACTGG 1 cut(s) 1334
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 4 cut(s) 240, 317, 825, 906
BssMI GATC 8 cut(s) 66, 551, 732, 1117, 1138, 1261, 1470, 1562
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 3 cut(s) 318, 852, 1023
Bst4CI ACNGT 6 cut(s) 149, 455, 746, 907, 1087, 1546
Bst6I CTCTTC 1 cut(s) 1347
BstAPI GCANNNNNTGC 1 cut(s) 224
BstAUI TGTACA 2 cut(s) 256, 1236
BstC8I GCNNGC 2 cut(s) 1196, 1486
BstDEI CTNAG 7 cut(s) 282, 381, 442, 689, 918, 1124, 1362
BstDSI CCRYGG 1 cut(s) 906
BstENI CCTNNNNNAGG 1 cut(s) 1068
BstF5I GGATG 4 cut(s) 495, 798, 850, 1048
BstFNI CGCG 1 cut(s) 510
BstHHI GCGC 3 cut(s) 510, 681, 1194
BstKTI GATC 8 cut(s) 69, 554, 735, 1120, 1141, 1264, 1473, 1565
BstMAI GTCTC 4 cut(s) 267, 353, 690, 780
BstMBI GATC 8 cut(s) 66, 551, 732, 1117, 1138, 1261, 1470, 1562
BstMCI CGRYCG 1 cut(s) 1417
BstMWI GCNNNNNNNGC 7 cut(s) 209, 218, 224, 313, 950, 1305, 1314
BstNI CCWGG 3 cut(s) 318, 852, 1023
BstNSI RCATGY 4 cut(s) 263, 776, 1293, 1453
BstSCI CCNGG 3 cut(s) 316, 850, 1021
BstSLI GKGCMC 1 cut(s) 697
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 5 cut(s) 230, 325, 921, 988, 1493
BstX2I RGATCY 2 cut(s) 1261, 1562
BstYI RGATCY 2 cut(s) 1261, 1562
BstZI CGGCCG 1 cut(s) 1414
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 2 cut(s) 953, 1416
BtgI CCRYGG 1 cut(s) 906
BtsCI GGATG 4 cut(s) 495, 798, 850, 1048
BtsI GCAGTG 2 cut(s) 1187, 1367
BtsIMutI CAGTG 5 cut(s) 154, 561, 697, 1187, 1367
BveI ACCTGC 2 cut(s) 1162, 1217
Cac8I GCNNGC 2 cut(s) 1196, 1486
CaiI CAGNNNCTG 1 cut(s) 1369
CciI TCATGA 1 cut(s) 63
CfoI GCGC 3 cut(s) 510, 681, 1194
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 1331
Csp6I GTAC 7 cut(s) 180, 257, 456, 848, 863, 1237, 1547
CspCI CAANNNNNGTGG 2 cut(s) 1356, 1391
CviQI GTAC 7 cut(s) 180, 257, 456, 848, 863, 1237, 1547
DdeI CTNAG 7 cut(s) 282, 381, 442, 689, 918, 1124, 1362
DpnI GATC 8 cut(s) 68, 553, 734, 1119, 1140, 1263, 1472, 1564
DpnII GATC 8 cut(s) 66, 551, 732, 1117, 1138, 1261, 1470, 1562
EaeI YGGCCR 2 cut(s) 951, 1414
EagI CGGCCG 1 cut(s) 1414
Eam1104I CTCTTC 1 cut(s) 1347
EarI CTCTTC 1 cut(s) 1347
EclXI CGGCCG 1 cut(s) 1414
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 2 cut(s) 353, 780
Eco47I GGWCC 1 cut(s) 1331
Eco52I CGGCCG 1 cut(s) 1414
Eco57I CTGAAG 1 cut(s) 852
Eco88I CYCGRG 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 1068
EcoRII CCWGG 3 cut(s) 316, 850, 1021
EcoT14I CCWWGG 1 cut(s) 240
EcoT22I ATGCAT 1 cut(s) 1048
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 240
FalI AAGNNNNNCTT 2 cut(s) 700, 732
FaqI GGGAC 2 cut(s) 696, 998
FauNDI CATATG 1 cut(s) 634
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 7 cut(s) 219, 314, 935, 954, 1002, 1417, 1482
FokI GGATG 4 cut(s) 482, 785, 857, 1055
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 7 cut(s) 219, 314, 935, 954, 1002, 1417, 1482
FspBI CTAG 1 cut(s) 876
FspI TGCGCA 2 cut(s) 680, 1193
GlaI GCGC 3 cut(s) 509, 680, 1193
GluI GCNGC 7 cut(s) 219, 314, 935, 954, 1002, 1417, 1482
GsaI CCCAGC 2 cut(s) 50, 471
GsuI CTGGAG 1 cut(s) 636
HaeIII GGCC 2 cut(s) 953, 1416
HapII CCGG 4 cut(s) 161, 204, 213, 1259
HhaI GCGC 3 cut(s) 510, 681, 1194
Hin6I GCGC 3 cut(s) 508, 679, 1192
HinP1I GCGC 3 cut(s) 508, 679, 1192
HinfI GANTC 5 cut(s) 368, 523, 922, 1175, 1462
HpaII CCGG 4 cut(s) 161, 204, 213, 1259
HphI GGTGA 7 cut(s) 314, 607, 786, 893, 1248, 1431, 1523
Hpy166II GTNNAC 2 cut(s) 257, 695
Hpy188I TCNGA 4 cut(s) 384, 921, 977, 1099
Hpy188III TCNNGA 3 cut(s) 64, 350, 1474
Hpy8I GTNNAC 2 cut(s) 257, 695
Hpy99I CGWCG 1 cut(s) 255
HpyAV CCTTC 8 cut(s) 239, 379, 761, 801, 876, 958, 1446, 1508
HpyCH4III ACNGT 6 cut(s) 149, 455, 746, 907, 1087, 1546
HpyF10VI GCNNNNNNNGC 7 cut(s) 209, 218, 224, 313, 950, 1305, 1314
HpyF3I CTNAG 7 cut(s) 282, 381, 442, 689, 918, 1124, 1362
HspAI GCGC 3 cut(s) 508, 679, 1192
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 8 cut(s) 66, 551, 732, 1117, 1138, 1261, 1470, 1562
LmnI GCTCC 2 cut(s) 643, 919
Lsp1109I GCAGC 5 cut(s) 230, 325, 921, 988, 1493
LweI GCATC 6 cut(s) 367, 1033, 1138, 1262, 1326, 1497
MaeI CTAG 1 cut(s) 876
MaeIII GTNAC 3 cut(s) 128, 556, 746
MalI GATC 8 cut(s) 68, 553, 734, 1119, 1140, 1263, 1472, 1564
MboI GATC 8 cut(s) 66, 551, 732, 1117, 1138, 1261, 1470, 1562
MboII GAAGA 4 cut(s) 52, 1100, 1112, 1334
MfeI CAATTG 1 cut(s) 372
MflI RGATCY 2 cut(s) 1261, 1562
MhlI GDGCHC 2 cut(s) 53, 697
MluCI AATT 5 cut(s) 294, 372, 539, 1029, 1165
MlyI GAGTC 2 cut(s) 377, 517
Mph1103I ATGCAT 1 cut(s) 1048
MseI TTAA 5 cut(s) 566, 1067, 1356, 1421, 1579
MslI CAYNNNNRTG 1 cut(s) 771
MspA1I CMGCKG 2 cut(s) 934, 1484
MspI CCGG 4 cut(s) 161, 204, 213, 1259
MspR9I CCNGG 3 cut(s) 318, 852, 1023
MunI CAATTG 1 cut(s) 372
Mva1269I GAATGC 2 cut(s) 510, 1046
MvaI CCWGG 3 cut(s) 318, 852, 1023
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 7 cut(s) 209, 218, 224, 313, 950, 1305, 1314
NdeI CATATG 1 cut(s) 634
NdeII GATC 8 cut(s) 66, 551, 732, 1117, 1138, 1261, 1470, 1562
NlaIV GGNNCC 4 cut(s) 202, 915, 1061, 1263
NmuCI GTSAC 2 cut(s) 556, 746
NsbI TGCGCA 2 cut(s) 680, 1193
NsiI ATGCAT 1 cut(s) 1048
NspI RCATGY 4 cut(s) 263, 776, 1293, 1453
PaeR7I CTCGAG 1 cut(s) 350
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 2 cut(s) 1162, 1217
PciI ACATGT 2 cut(s) 259, 772
PctI GAATGC 2 cut(s) 510, 1046
PfeI GAWTC 3 cut(s) 922, 1175, 1462
PkrI GCNGC 7 cut(s) 220, 315, 936, 955, 1003, 1418, 1483
PleI GAGTC 2 cut(s) 376, 517
PpsI GAGTC 2 cut(s) 376, 517
PscI ACATGT 2 cut(s) 259, 772
PshBI ATTAAT 1 cut(s) 566
PsiI TTATAA 1 cut(s) 1505
Psp6I CCWGG 3 cut(s) 316, 850, 1021
PspFI CCCAGC 2 cut(s) 46, 467
PspGI CCWGG 3 cut(s) 316, 850, 1021
PspN4I GGNNCC 4 cut(s) 202, 915, 1061, 1263
PspPI GGNCC 1 cut(s) 1331
PstNI CAGNNNCTG 1 cut(s) 1369
PsuI RGATCY 2 cut(s) 1261, 1562
PvuII CAGCTG 2 cut(s) 934, 1484
RsaI GTAC 7 cut(s) 181, 258, 457, 849, 864, 1238, 1548
RsaNI GTAC 7 cut(s) 180, 257, 456, 848, 863, 1237, 1547
RseI CAYNNNNRTG 1 cut(s) 771
SaqAI TTAA 5 cut(s) 566, 1067, 1356, 1421, 1579
SatI GCNGC 7 cut(s) 219, 314, 935, 954, 1002, 1417, 1482
Sau3AI GATC 8 cut(s) 66, 551, 732, 1117, 1138, 1261, 1470, 1562
Sau96I GGNCC 1 cut(s) 1331
SchI GAGTC 2 cut(s) 377, 517
ScrFI CCNGG 3 cut(s) 318, 852, 1023
SduI GDGCHC 2 cut(s) 53, 697
SfaNI GCATC 6 cut(s) 367, 1033, 1138, 1262, 1326, 1497
Sfr274I CTCGAG 1 cut(s) 350
SinI GGWCC 1 cut(s) 1331
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 3 cut(s) 329, 350, 1474
SmoI CTYRAG 3 cut(s) 329, 350, 1474
Sse9I AATT 5 cut(s) 294, 372, 539, 1029, 1165
SsiI CCGC 2 cut(s) 954, 1417
SspMI CTAG 1 cut(s) 876
StyD4I CCNGG 3 cut(s) 316, 850, 1021
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 6 cut(s) 149, 455, 746, 907, 1087, 1546
TaqI TCGA 4 cut(s) 267, 351, 570, 1465
TasI AATT 5 cut(s) 294, 372, 539, 1029, 1165
TatI WGTACW 3 cut(s) 256, 455, 1236
TauI GCSGC 2 cut(s) 956, 1419
TfiI GAWTC 3 cut(s) 922, 1175, 1462
Tru1I TTAA 5 cut(s) 566, 1067, 1356, 1421, 1579
Tru9I TTAA 5 cut(s) 566, 1067, 1356, 1421, 1579
TscAI CASTG 5 cut(s) 154, 561, 697, 1194, 1374
TseFI GTSAC 2 cut(s) 556, 746
TseI GCWGC 5 cut(s) 218, 313, 934, 1001, 1481
Tsp45I GTSAC 2 cut(s) 556, 746
TspDTI ATGAA 6 cut(s) 30, 52, 188, 484, 1314, 1565
TspGWI ACGGA 2 cut(s) 1103, 1344
TspRI CASTG 5 cut(s) 154, 561, 697, 1194, 1374
VneI GTGCAC 1 cut(s) 693
VpaK11BI GGWCC 1 cut(s) 1331
VspI ATTAAT 1 cut(s) 566
XagI CCTNNNNNAGG 1 cut(s) 1068
XceI RCATGY 4 cut(s) 263, 776, 1293, 1453
XcmI CCANNNNNNNNNTGG 1 cut(s) 1219
XhoI CTCGAG 1 cut(s) 350
XspI CTAG 1 cut(s) 876
Zsp2I ATGCAT 1 cut(s) 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.