MD05G1111800.v1.1

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000108
Physical Location & Seq
Reverse (-)
22879568 .. 22882729
3162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1111800.v1.1.491

Sequence Viewer

Length: 1563 bp
ATGGTTTACCTGAATATAGTGTGTGCTGTTATAGCCTCGCTGCTCGGGTTTGTTTTCTTGTGCAGTCTAAGACGACAGAAGAAGGCCAGAGCTCATCAAAATGGGAATTGTTTGAAGAGCTGTGAAAATGGAACGTGCGACGAGGCAAAGATGGTTGAAAGTGCAGACATTATCGTTGTTGGTGCTGGGGTTGTTGGTTCGGCGCTCGCTTACACTCTTGGGAAGGATGGGCGCCGAGTGCATGTGATTGAAAGGGACTTGAGTGAGCCAGACAGAATCGTCGGCGAGCTGCTGCAACCAGGGGGATATCTCAGATTAGTTGAGTTAGGCCTTGAGGATTGTGTAAATGAGATTGATGCTCAACGGGTTTTCGGATATGCCCTTTACAAGGATGGAAAAAGTACCAAGCTGCCATATCCTTTGGAAAACTTCCATCCTGATGTGGCTGGGAGAAGCTTTCACCACGGGCGTTTCATTCAAAGGATGCGGGAAAAAGCTTCATCTCTTCCCAATGTAAGATTGGAACAAGGAACGGTGACATCTCTACTGGAAGAAAAAGGTACTGTCAAGGGGGTGCAGTATAAATCCAAAGGTAGGGATCTAGAACTCAGTGCACACGCGCCTCTCACAATAGTATGCGATGGATGCTATTCAAACCTACGGCACTCTCTCTGCAATCCTAAGGTTGATATACCTTCATGTTTTGTTGGTTTGGTTCTGGAGAATTGCCAGCTTCCATATGCAAATCACGGGCATGTAATATTGGCAGATCCTTCGCCGATATTGTTTTATCCTATAAGCAGCACCGAGATTCGTTGCCTTGTTGATGTACCAGGCCAAAAAGTACCTTCTATTTCTGGTGGTGAAATGTCTCGCTACTTGAAAACAAAAGTGGCACCCCAAGTTCCCCCTGAGCTGTATGCCGCTTTTATGGCTGCCGCGGATAAAGGAAACATAAAATCAATGCCAAACAGGAGCATGCCTGCTGCTCCTCGTCCCACTCCTGGTGTCCTTCTGATGGGGGACGCTGTCAACATGAGGCATCCTCTAACTGGAGGAGGCATGACTGTGGCTCTTTCAGACATTGTTGTTCTTAGAAATCTTCTAAGACCTCTGCACAATCTGAATGATGCACCTGCCCTTTGTAAATACCTCGAATCCTTCTATACGCTGCGCAAGCCTGTGGCCTCAACCATAAACACATTGGCAGGTGCACTATACAGGGTATTTTGTGCATCACCTGATCCGGCAAGGATGGAAATGCGCCAAGCATGTTTCGATTACTTGAGCCTTGGAGGCGTGTTCTCGAATGGACCTCTAGCACTTCTTTCCGGCCTAAACCCGCAACCATTGAGCTTGGTTCTCCACTTTTTTGCCGTGGCTGTCTACGCCATTGGGCGCTTGCTACTTCCATTCCCTTGCCCAAAACGCATGTGGATTGGAGCCAGATTGGTTTTGGGTGCTTCAGGAATCATATTCCCCATAATCAACTCTGAAGGAGTGAGGCAAATGTTCTTCCCTGTGACAGTGGCATCATATTACAGAACTCCTCCTATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

521

Amino Acids

56.72

Weight (kDa)

8.96

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 55 - 369 2.5e-16 FAD binding domain
DAO PF01266 56 - 86 3.6e-06 FAD dependent oxidoreductase
SE PF08491 206 - 478 3e-117 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1144, 1199
AasI GACNNNNNNGTC 1 cut(s) 278
Acc16I TGCGCA 1 cut(s) 1175
Acc36I ACCTGC 2 cut(s) 1144, 1199
AccB1I GGYRCC 2 cut(s) 231, 895
AccI GTMKAC 1 cut(s) 1386
AccII CGCG 2 cut(s) 620, 941
AciI CCGC 5 cut(s) 487, 924, 939, 941, 1343
AclWI GGATC 3 cut(s) 606, 764, 1238
AcuI CTGAAG 2 cut(s) 1449, 1515
AcyI GRCGYC 1 cut(s) 232
AfaI GTAC 4 cut(s) 403, 562, 831, 846
AfiI CCNNNNNNNGG 5 cut(s) 388, 594, 1004, 1018, 1052
AgsI TTSAA 6 cut(s) 115, 158, 251, 479, 654, 883
AjnI CCWGG 3 cut(s) 298, 832, 1003
AluBI AGCT 9 cut(s) 92, 120, 289, 409, 456, 497, 733, 916, 1356
AluI AGCT 9 cut(s) 92, 120, 289, 409, 456, 497, 733, 916, 1356
Alw21I GWGCWC 3 cut(s) 94, 616, 1216
Alw26I GTCTC 1 cut(s) 876
Alw44I GTGCAC 2 cut(s) 612, 1212
AlwI GGATC 3 cut(s) 606, 764, 1238
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 5 cut(s) 84, 328, 835, 1185, 1333
ApaLI GTGCAC 2 cut(s) 612, 1212
ApeKI GCWGC 8 cut(s) 40, 289, 292, 409, 801, 935, 986, 1171
AseI ATTAAT 1 cut(s) 1557
AspLEI GCGC 6 cut(s) 205, 234, 622, 1176, 1266, 1401
AspS9I GGNCC 1 cut(s) 1313
AsuHPI GGTGA 4 cut(s) 452, 547, 875, 1230
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 1 cut(s) 1313
AxyI CCTNAGG 1 cut(s) 681
BaeGI GKGCMC 2 cut(s) 616, 1216
BanI GGYRCC 2 cut(s) 231, 895
BanII GRGCYC 1 cut(s) 94
Bbv12I GWGCWC 3 cut(s) 94, 616, 1216
BbvI GCAGC 8 cut(s) 27, 276, 279, 396, 813, 922, 973, 1158
BccI CCATC 7 cut(s) 145, 221, 386, 441, 635, 1012, 1249
BceAI ACGGC 2 cut(s) 677, 1361
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciT130I CCWGG 3 cut(s) 300, 834, 1005
BcoDI GTCTC 1 cut(s) 876
BfaI CTAG 2 cut(s) 602, 1319
BfoI RGCGCY 3 cut(s) 206, 235, 1402
BfuAI ACCTGC 2 cut(s) 1144, 1199
BglI GCCNNNNNGGC 1 cut(s) 1296
Bme1390I CCNGG 3 cut(s) 300, 834, 1005
Bme18I GGWCC 1 cut(s) 1313
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 1313
BmiI GGNNCC 3 cut(s) 233, 897, 1444
BmrFI CCNGG 3 cut(s) 300, 834, 1005
BmsI GCATC 7 cut(s) 346, 474, 635, 1051, 1120, 1244, 1541
BplI GAGNNNNNCTC 2 cut(s) 1030, 1062
BpmI CTGGAG 2 cut(s) 740, 1074
Bpu10I CCTNAGC 1 cut(s) 912
BpuEI CTTGAG 3 cut(s) 280, 353, 1306
BsaHI GRCGYC 1 cut(s) 232
BsaJI CCNNGG 5 cut(s) 299, 463, 939, 1291, 1377
Bsc4I CCNNNNNNNGG 5 cut(s) 388, 594, 1004, 1018, 1052
Bse1I ACTGG 2 cut(s) 552, 1057
Bse21I CCTNAGG 1 cut(s) 681
BseBI CCWGG 3 cut(s) 300, 834, 1005
BseDI CCNNGG 5 cut(s) 299, 463, 939, 1291, 1377
BseGI GGATG 7 cut(s) 232, 397, 433, 489, 650, 1042, 1260
BseLI CCNNNNNNNGG 5 cut(s) 388, 594, 1004, 1018, 1052
BseMII CTCAG 3 cut(s) 325, 622, 903
BseNI ACTGG 2 cut(s) 552, 1057
BseRI GAGGAG 3 cut(s) 981, 1071, 1539
BseSI GKGCMC 2 cut(s) 616, 1216
BseXI GCAGC 8 cut(s) 27, 276, 279, 396, 813, 922, 973, 1158
BseYI CCCAGC 2 cut(s) 185, 446
BsgI GTGCAG 4 cut(s) 82, 183, 596, 1100
Bsh1236I CGCG 2 cut(s) 620, 941
BshFI GGCC 5 cut(s) 86, 330, 837, 1187, 1335
BshNI GGYRCC 2 cut(s) 231, 895
BsiHKAI GWGCWC 3 cut(s) 94, 616, 1216
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 2 cut(s) 1247, 1332
BslFI GGGAC 3 cut(s) 269, 981, 1037
BslI CCNNNNNNNGG 5 cut(s) 388, 594, 1004, 1018, 1052
BsmAI GTCTC 1 cut(s) 876
BsmFI GGGAC 3 cut(s) 269, 981, 1037
BsnI GGCC 5 cut(s) 86, 330, 837, 1187, 1335
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 3 cut(s) 94, 616, 1216
Bsp143I GATC 3 cut(s) 598, 769, 1243
BspACI CCGC 5 cut(s) 487, 924, 939, 941, 1343
BspANI GGCC 5 cut(s) 86, 330, 837, 1187, 1335
BspCNI CTCAG 3 cut(s) 324, 621, 904
BspFNI CGCG 2 cut(s) 620, 941
BspLI GGNNCC 3 cut(s) 233, 897, 1444
BspMI ACCTGC 2 cut(s) 1144, 1199
BspPI GGATC 3 cut(s) 606, 764, 1238
BspQI GCTCTTC 1 cut(s) 110
BspT107I GGYRCC 2 cut(s) 231, 895
BsrI ACTGG 2 cut(s) 552, 1057
BssECI CCNNGG 5 cut(s) 299, 463, 939, 1291, 1377
BssMI GATC 3 cut(s) 598, 769, 1243
BssNI GRCGYC 1 cut(s) 232
BssT1I CCWWGG 1 cut(s) 1291
Bst2UI CCWGG 3 cut(s) 300, 834, 1005
Bst4CI ACNGT 4 cut(s) 535, 565, 1069, 1528
Bst6I CTCTTC 2 cut(s) 110, 510
BstACI GRCGYC 1 cut(s) 232
BstC8I GCNNGC 7 cut(s) 207, 287, 731, 980, 984, 1178, 1403
BstDEI CTNAG 7 cut(s) 68, 311, 608, 681, 912, 1094, 1106
BstDSI CCRYGG 3 cut(s) 463, 939, 1377
BstENI CCTNNNNNAGG 1 cut(s) 386
BstF5I GGATG 7 cut(s) 232, 397, 433, 489, 650, 1042, 1260
BstFNI CGCG 2 cut(s) 620, 941
BstH2I RGCGCY 3 cut(s) 206, 235, 1402
BstHHI GCGC 6 cut(s) 205, 234, 622, 1176, 1266, 1401
BstKTI GATC 3 cut(s) 601, 772, 1246
BstMAI GTCTC 1 cut(s) 876
BstMBI GATC 3 cut(s) 598, 769, 1243
BstMWI GCNNNNNNNGC 8 cut(s) 32, 238, 645, 932, 1177, 1296, 1388, 1428
BstNI CCWGG 3 cut(s) 300, 834, 1005
BstNSI RCATGY 5 cut(s) 245, 758, 982, 1275, 1435
BstSCI CCNGG 3 cut(s) 298, 832, 1003
BstSLI GKGCMC 2 cut(s) 616, 1216
BstUI CGCG 2 cut(s) 620, 941
BstV1I GCAGC 8 cut(s) 27, 276, 279, 396, 813, 922, 973, 1158
BstX2I RGATCY 2 cut(s) 598, 769
BstYI RGATCY 2 cut(s) 598, 769
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 5 cut(s) 86, 330, 837, 1187, 1335
BtgI CCRYGG 3 cut(s) 463, 939, 1377
BtgZI GCGATG 1 cut(s) 654
BtsCI GGATG 7 cut(s) 232, 397, 433, 489, 650, 1042, 1260
BtsIMutI CAGTG 2 cut(s) 616, 1533
BveI ACCTGC 2 cut(s) 1144, 1199
Cac8I GCNNGC 7 cut(s) 207, 287, 731, 980, 984, 1178, 1403
CfoI GCGC 6 cut(s) 205, 234, 622, 1176, 1266, 1401
Cfr13I GGNCC 1 cut(s) 1313
Cfr42I CCGCGG 1 cut(s) 942
CseI GACGC 1 cut(s) 1034
Csp6I GTAC 4 cut(s) 402, 561, 830, 845
CviAII CATG 8 cut(s) 242, 699, 755, 979, 1036, 1063, 1272, 1432
CviQI GTAC 4 cut(s) 402, 561, 830, 845
DdeI CTNAG 7 cut(s) 68, 311, 608, 681, 912, 1094, 1106
DinI GGCGCC 1 cut(s) 233
DpnI GATC 3 cut(s) 600, 771, 1245
DpnII GATC 3 cut(s) 598, 769, 1243
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
Eam1104I CTCTTC 2 cut(s) 110, 510
EarI CTCTTC 2 cut(s) 110, 510
Ecl136II GAGCTC 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 1291
Eco147I AGGCCT 1 cut(s) 330
Eco24I GRGCYC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 1313
Eco53kI GAGCTC 1 cut(s) 92
Eco57I CTGAAG 2 cut(s) 1449, 1515
Eco81I CCTNAGG 1 cut(s) 681
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 92
EcoNI CCTNNNNNAGG 1 cut(s) 386
EcoRII CCWGG 3 cut(s) 298, 832, 1003
EcoRV GATATC 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 1291
EcoT38I GRGCYC 1 cut(s) 94
EgeI GGCGCC 1 cut(s) 233
EheI GGCGCC 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 1291
FaeI CATG 8 cut(s) 245, 702, 758, 982, 1039, 1066, 1275, 1435
FaqI GGGAC 3 cut(s) 269, 981, 1037
FatI CATG 8 cut(s) 241, 698, 754, 978, 1035, 1062, 1271, 1431
FauI CCCGC 2 cut(s) 480, 1350
FauNDI CATATG 1 cut(s) 739
FblI GTMKAC 1 cut(s) 1386
FokI GGATG 7 cut(s) 239, 404, 420, 496, 657, 1029, 1267
FriOI GRGCYC 1 cut(s) 94
FspBI CTAG 2 cut(s) 602, 1319
FspI TGCGCA 1 cut(s) 1175
GlaI GCGC 6 cut(s) 204, 233, 621, 1175, 1265, 1400
GsaI CCCAGC 2 cut(s) 189, 450
GsuI CTGGAG 2 cut(s) 740, 1074
HaeII RGCGCY 3 cut(s) 206, 235, 1402
HaeIII GGCC 5 cut(s) 86, 330, 837, 1187, 1335
HapII CCGG 2 cut(s) 1247, 1332
HgaI GACGC 1 cut(s) 1034
HhaI GCGC 6 cut(s) 205, 234, 622, 1176, 1266, 1401
Hin1I GRCGYC 1 cut(s) 232
Hin1II CATG 8 cut(s) 245, 702, 758, 982, 1039, 1066, 1275, 1435
Hin6I GCGC 6 cut(s) 203, 232, 620, 1174, 1264, 1399
HinP1I GCGC 6 cut(s) 203, 232, 620, 1174, 1264, 1399
HincII GTYRAC 1 cut(s) 1033
HindII GTYRAC 1 cut(s) 1033
HindIII AAGCTT 2 cut(s) 454, 495
HinfI GANTC 4 cut(s) 276, 811, 1157, 1470
HpaII CCGG 2 cut(s) 1247, 1332
HphI GGTGA 4 cut(s) 452, 547, 875, 1230
Hpy166II GTNNAC 5 cut(s) 7, 614, 1033, 1214, 1387
Hpy188I TCNGA 6 cut(s) 314, 374, 1017, 1081, 1125, 1495
Hpy188III TCNNGA 5 cut(s) 437, 602, 719, 1306, 1467
Hpy8I GTNNAC 5 cut(s) 7, 614, 1033, 1214, 1387
Hpy99I CGWCG 2 cut(s) 143, 284
HpyAV CCTTC 8 cut(s) 76, 217, 705, 783, 858, 1022, 1171, 1490
HpyCH4III ACNGT 4 cut(s) 535, 565, 1069, 1528
HpyCH4IV ACGT 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 238, 645, 932, 1177, 1296, 1388, 1428
HpyF3I CTNAG 7 cut(s) 68, 311, 608, 681, 912, 1094, 1106
HpySE526I ACGT 1 cut(s) 134
Hsp92I GRCGYC 1 cut(s) 232
Hsp92II CATG 8 cut(s) 245, 702, 758, 982, 1039, 1066, 1275, 1435
HspAI GCGC 6 cut(s) 203, 232, 620, 1174, 1264, 1399
KasI GGCGCC 1 cut(s) 231
KspI CCGCGG 1 cut(s) 942
Kzo9I GATC 3 cut(s) 598, 769, 1243
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 3 cut(s) 975, 994, 1442
Lsp1109I GCAGC 8 cut(s) 27, 276, 279, 396, 813, 922, 973, 1158
LweI GCATC 7 cut(s) 346, 474, 635, 1051, 1120, 1244, 1541
MaeI CTAG 2 cut(s) 602, 1319
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 2 cut(s) 535, 1522
MalI GATC 3 cut(s) 600, 771, 1245
MboI GATC 3 cut(s) 598, 769, 1243
MboII GAAGA 6 cut(s) 91, 127, 497, 563, 1094, 1507
MflI RGATCY 2 cut(s) 598, 769
MhlI GDGCHC 3 cut(s) 94, 616, 1216
MluCI AATT 3 cut(s) 106, 724, 1558
Mly113I GGCGCC 1 cut(s) 232
MseI TTAA 2 cut(s) 1557, 1561
MslI CAYNNNNRTG 4 cut(s) 99, 438, 753, 1067
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 2 cut(s) 1247, 1332
MspR9I CCNGG 3 cut(s) 300, 834, 1005
MvaI CCWGG 3 cut(s) 300, 834, 1005
MvnI CGCG 2 cut(s) 620, 941
MwoI GCNNNNNNNGC 8 cut(s) 32, 238, 645, 932, 1177, 1296, 1388, 1428
NarI GGCGCC 1 cut(s) 232
NdeI CATATG 1 cut(s) 739
NdeII GATC 3 cut(s) 598, 769, 1243
NlaIII CATG 8 cut(s) 245, 702, 758, 982, 1039, 1066, 1275, 1435
NlaIV GGNNCC 3 cut(s) 233, 897, 1444
NmeAIII GCCGAG 1 cut(s) 260
NmuCI GTSAC 2 cut(s) 535, 1522
NsbI TGCGCA 1 cut(s) 1175
NspI RCATGY 5 cut(s) 245, 758, 982, 1275, 1435
PacI TTAATTAA 1 cut(s) 1561
PaeI GCATGC 1 cut(s) 982
PaqCI CACCTGC 2 cut(s) 1144, 1199
PceI AGGCCT 1 cut(s) 330
PciSI GCTCTTC 1 cut(s) 110
PfeI GAWTC 4 cut(s) 276, 811, 1157, 1470
PflFI GACNNNGTC 1 cut(s) 1028
PluTI GGCGCC 1 cut(s) 235
PshBI ATTAAT 1 cut(s) 1557
Psp124BI GAGCTC 1 cut(s) 94
Psp6I CCWGG 3 cut(s) 298, 832, 1003
PspFI CCCAGC 2 cut(s) 185, 446
PspGI CCWGG 3 cut(s) 298, 832, 1003
PspN4I GGNNCC 3 cut(s) 233, 897, 1444
PspPI GGNCC 1 cut(s) 1313
PsuI RGATCY 2 cut(s) 598, 769
PsyI GACNNNGTC 1 cut(s) 1028
RsaI GTAC 4 cut(s) 403, 562, 831, 846
RsaNI GTAC 4 cut(s) 402, 561, 830, 845
RseI CAYNNNNRTG 4 cut(s) 99, 438, 753, 1067
SacI GAGCTC 1 cut(s) 94
SacII CCGCGG 1 cut(s) 942
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 2 cut(s) 1557, 1561
Sau3AI GATC 3 cut(s) 598, 769, 1243
Sau96I GGNCC 1 cut(s) 1313
ScrFI CCNGG 3 cut(s) 300, 834, 1005
SduI GDGCHC 3 cut(s) 94, 616, 1216
SfaNI GCATC 7 cut(s) 346, 474, 635, 1051, 1120, 1244, 1541
SfoI GGCGCC 1 cut(s) 233
Sfr303I CCGCGG 1 cut(s) 942
SgrBI CCGCGG 1 cut(s) 942
SinI GGWCC 1 cut(s) 1313
SmiMI CAYNNNNRTG 4 cut(s) 99, 438, 753, 1067
SmlI CTYRAG 3 cut(s) 259, 332, 1285
SmoI CTYRAG 3 cut(s) 259, 332, 1285
SphI GCATGC 1 cut(s) 982
Sse9I AATT 3 cut(s) 106, 724, 1558
SseBI AGGCCT 1 cut(s) 330
SsiI CCGC 5 cut(s) 487, 924, 939, 941, 1343
SspDI GGCGCC 1 cut(s) 231
SspI AATATT 1 cut(s) 762
SspMI CTAG 2 cut(s) 602, 1319
SstI GAGCTC 1 cut(s) 94
StuI AGGCCT 1 cut(s) 330
StyD4I CCNGG 3 cut(s) 298, 832, 1003
StyI CCWWGG 1 cut(s) 1291
TaaI ACNGT 4 cut(s) 535, 565, 1069, 1528
TaiI ACGT 1 cut(s) 137
TaqI TCGA 3 cut(s) 1155, 1278, 1307
TasI AATT 3 cut(s) 106, 724, 1558
TauI GCSGC 2 cut(s) 926, 941
TfiI GAWTC 4 cut(s) 276, 811, 1157, 1470
Tru1I TTAA 2 cut(s) 1557, 1561
Tru9I TTAA 2 cut(s) 1557, 1561
TscAI CASTG 2 cut(s) 616, 1533
TseFI GTSAC 2 cut(s) 535, 1522
TseI GCWGC 8 cut(s) 40, 289, 292, 409, 801, 935, 986, 1171
Tsp45I GTSAC 2 cut(s) 535, 1522
TspDTI ATGAA 3 cut(s) 463, 489, 687
TspRI CASTG 2 cut(s) 616, 1533
Tth111I GACNNNGTC 1 cut(s) 1028
VneI GTGCAC 2 cut(s) 612, 1212
VpaK11BI GGWCC 1 cut(s) 1313
VspI ATTAAT 1 cut(s) 1557
XagI CCTNNNNNAGG 1 cut(s) 386
XbaI TCTAGA 1 cut(s) 601
XceI RCATGY 5 cut(s) 245, 758, 982, 1275, 1435
XcmI CCANNNNNNNNNTGG 4 cut(s) 517, 1201, 1431, 1453
XmiI GTMKAC 1 cut(s) 1386
XspI CTAG 2 cut(s) 602, 1319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.