Rh6AG035000

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000108
Physical Location & Seq
Reverse (-)
4423517 .. 4426653
3137 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG035000.1

Sequence Viewer

Length: 759 bp
ATGCGTGAAAAAGCTGAAACTCTTTCAAATGTGAAATTGGAACAAGGATCAGCGACAACATTGATCGAGGAAAAGGGCATTGTCAAAGGGGTGATTTACAAAAACAAGGCTGGAGAGGAGATGAGAACATATGCTCCACTAACAATCGTGTGTGATGGCTGCTTTTCAAATCTACGCAAATCACTTAGTGCACCAAAGGTTGAAAGTCCCTCTTGCTTTGTTGGTTTGATCTTGGAAAATTGTGAGCTGCCACACGCAAATCATGGACATGTCATTTTGGGAGACCCTTCACCCATCCTGTTTTATCCTATCAGTAGCACCGAGATTCGCTGTTTGGTCGATGTACCTGGAACAAAAGTACCTTCAGTAGCTAGTGGTGAAATGGCTAGCTATCTGAAAACTGTTGTGGCTCCTCAGGTTCCCCCACAGCTGTACAATGCTTTTATGGTTGCAGTAGAGAAAGGAAACATTAGATCCATGCAAAACAAAAGCATGGCTGCTAATCCTGTTCCCACTCCTGGTGCAATTTTGTTAGGGGATTCATTCAACATGAGGCATCCTTTAACAGGAGGAGGAATGACCGTGGCTCTTTCAGACATTGTTCTTCTCCGCGATCTTCTTAGACCCCTGCGTGATCTCAATGATGCACTGGCCTTGTGCATTTACCTTGAATCATTCTACACACTTCGGAAGGGACTGGAACATGGAATCCACCTCAAGGCAACCACTGCTTTGGCTTACCATTTTTTGCAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

252

Amino Acids

27.41

Weight (kDa)

7.64

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 1 - 204 1.7e-07 FAD binding domain
SE PF08491 45 - 237 7.7e-82 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 612
AciI CCGC 1 cut(s) 610
AclWI GGATC 2 cut(s) 55, 468
AcuI CTGAAG 1 cut(s) 348
AdeI CACNNNGTG 1 cut(s) 188
AfaI GTAC 3 cut(s) 345, 360, 434
AfiI CCNNNNNNNGG 3 cut(s) 518, 566, 718
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 5 cut(s) 27, 168, 203, 547, 671
AjnI CCWGG 2 cut(s) 346, 517
AloI GAACNNNNNNTCC 4 cut(s) 118, 150, 693, 725
AluBI AGCT 5 cut(s) 14, 247, 371, 390, 430
AluI AGCT 5 cut(s) 14, 247, 371, 390, 430
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 1 cut(s) 276
Alw44I GTGCAC 1 cut(s) 189
AlwI GGATC 2 cut(s) 55, 468
AoxI GGCC 1 cut(s) 651
ApaLI GTGCAC 1 cut(s) 189
ApeKI GCWGC 3 cut(s) 159, 247, 497
AsuHPI GGTGA 3 cut(s) 103, 282, 389
AsuNHI GCTAGC 1 cut(s) 386
AxyI CCTNAGG 1 cut(s) 414
BaeGI GKGCMC 1 cut(s) 193
BaeI ACNNNNGTAYC 2 cut(s) 342, 375
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 3 cut(s) 146, 234, 484
BccI CCATC 2 cut(s) 149, 302
BciT130I CCWGG 2 cut(s) 348, 519
BcoDI GTCTC 1 cut(s) 276
BfaI CTAG 3 cut(s) 372, 387, 757
BisI GCNGC 3 cut(s) 160, 248, 498
BlsI GCNGC 3 cut(s) 161, 249, 499
Bme1390I CCNGG 2 cut(s) 348, 519
BmiI GGNNCC 2 cut(s) 411, 420
BmrFI CCNGG 2 cut(s) 348, 519
BmsI GCATC 2 cut(s) 565, 634
BmtI GCTAGC 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 701
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 582
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bsc4I CCNNNNNNNGG 3 cut(s) 518, 566, 718
Bse1I ACTGG 2 cut(s) 654, 702
Bse21I CCTNAGG 1 cut(s) 414
BseBI CCWGG 2 cut(s) 348, 519
BseDI CCNNGG 1 cut(s) 582
BseGI GGATG 2 cut(s) 294, 556
BseLI CCNNNNNNNGG 3 cut(s) 518, 566, 718
BseMII CTCAG 1 cut(s) 428
BseNI ACTGG 2 cut(s) 654, 702
BseRI GAGGAG 3 cut(s) 131, 402, 585
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 3 cut(s) 146, 234, 484
Bsh1236I CGCG 1 cut(s) 612
BshFI GGCC 1 cut(s) 653
BsiHKAI GWGCWC 1 cut(s) 193
BslFI GGGAC 2 cut(s) 192, 708
BslI CCNNNNNNNGG 3 cut(s) 518, 566, 718
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 2 cut(s) 192, 708
BsnI GGCC 1 cut(s) 653
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 193
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 6 cut(s) 47, 63, 228, 473, 613, 634
BspACI CCGC 1 cut(s) 610
BspANI GGCC 1 cut(s) 653
BspCNI CTCAG 1 cut(s) 427
BspFNI CGCG 1 cut(s) 612
BspLI GGNNCC 2 cut(s) 411, 420
BspOI GCTAGC 1 cut(s) 390
BspPI GGATC 2 cut(s) 55, 468
BspTNI GGTCTC 1 cut(s) 276
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 2 cut(s) 654, 702
BssECI CCNNGG 1 cut(s) 582
BssMI GATC 6 cut(s) 47, 63, 228, 473, 613, 634
Bst2UI CCWGG 2 cut(s) 348, 519
Bst4CI ACNGT 2 cut(s) 403, 583
BstAPI GCANNNNNTGC 1 cut(s) 728
BstAUI TGTACA 1 cut(s) 432
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 3 cut(s) 185, 414, 620
BstDSI CCRYGG 1 cut(s) 582
BstENI CCTNNNNNAGG 1 cut(s) 564
BstF5I GGATG 2 cut(s) 294, 556
BstFNI CGCG 1 cut(s) 612
BstKTI GATC 6 cut(s) 50, 66, 231, 476, 616, 637
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 6 cut(s) 47, 63, 228, 473, 613, 634
BstMWI GCNNNNNNNGC 1 cut(s) 728
BstNI CCWGG 2 cut(s) 348, 519
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 346, 517
BstSLI GKGCMC 1 cut(s) 193
BstUI CGCG 1 cut(s) 612
BstV1I GCAGC 3 cut(s) 146, 234, 484
BstX2I RGATCY 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 733
BstYI RGATCY 1 cut(s) 473
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 1 cut(s) 653
BtgI CCRYGG 1 cut(s) 582
BtsCI GGATG 2 cut(s) 294, 556
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 2 cut(s) 647, 726
Cac8I GCNNGC 1 cut(s) 388
Csp6I GTAC 3 cut(s) 344, 359, 433
CviAII CATG 6 cut(s) 263, 269, 478, 493, 550, 704
CviQI GTAC 3 cut(s) 344, 359, 433
DdeI CTNAG 3 cut(s) 185, 414, 620
DpnI GATC 6 cut(s) 49, 65, 230, 475, 615, 636
DpnII GATC 6 cut(s) 47, 63, 228, 473, 613, 634
DraIII CACNNNGTG 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 276
Eco57I CTGAAG 1 cut(s) 348
Eco81I CCTNAGG 1 cut(s) 414
EcoNI CCTNNNNNAGG 1 cut(s) 564
EcoRII CCWGG 2 cut(s) 346, 517
FaeI CATG 6 cut(s) 266, 272, 481, 496, 553, 707
FaiI YATR 9 cut(s) 130, 132, 264, 270, 446, 479, 494, 551, 705
FalI AAGNNNNNCTT 2 cut(s) 196, 228
FaqI GGGAC 2 cut(s) 192, 708
FatI CATG 6 cut(s) 262, 268, 477, 492, 549, 703
FauNDI CATATG 1 cut(s) 130
Fnu4HI GCNGC 3 cut(s) 160, 248, 498
FokI GGATG 2 cut(s) 281, 543
Fsp4HI GCNGC 3 cut(s) 160, 248, 498
FspBI CTAG 3 cut(s) 372, 387, 757
GluI GCNGC 3 cut(s) 160, 248, 498
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 1 cut(s) 653
Hin1II CATG 6 cut(s) 266, 272, 481, 496, 553, 707
HinfI GANTC 4 cut(s) 325, 539, 671, 708
HphI GGTGA 3 cut(s) 103, 282, 389
Hpy166II GTNNAC 1 cut(s) 191
Hpy188I TCNGA 3 cut(s) 396, 595, 690
Hpy8I GTNNAC 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 297, 372, 685
HpyCH4III ACNGT 2 cut(s) 403, 583
HpyCH4V TGCA 7 cut(s) 191, 452, 481, 524, 647, 660, 751
HpyF10VI GCNNNNNNNGC 1 cut(s) 728
HpyF3I CTNAG 3 cut(s) 185, 414, 620
Hsp92II CATG 6 cut(s) 266, 272, 481, 496, 553, 707
Kzo9I GATC 6 cut(s) 47, 63, 228, 473, 613, 634
LmnI GCTCC 2 cut(s) 139, 415
Lsp1109I GCAGC 3 cut(s) 146, 234, 484
LweI GCATC 2 cut(s) 565, 634
MaeI CTAG 3 cut(s) 372, 387, 757
MalI GATC 6 cut(s) 49, 65, 230, 475, 615, 636
MboI GATC 6 cut(s) 47, 63, 228, 473, 613, 634
MboII GAAGA 2 cut(s) 596, 608
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 3 cut(s) 35, 238, 525
MnlI CCTC 8 cut(s) 61, 109, 220, 423, 546, 563, 566, 725
MseI TTAA 1 cut(s) 563
MslI CAYNNNNRTG 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 430
MspR9I CCNGG 2 cut(s) 348, 519
MvaI CCWGG 2 cut(s) 348, 519
MvnI CGCG 1 cut(s) 612
MwoI GCNNNNNNNGC 1 cut(s) 728
NdeI CATATG 1 cut(s) 130
NdeII GATC 6 cut(s) 47, 63, 228, 473, 613, 634
NheI GCTAGC 1 cut(s) 386
NlaIII CATG 6 cut(s) 266, 272, 481, 496, 553, 707
NlaIV GGNNCC 2 cut(s) 411, 420
NspI RCATGY 1 cut(s) 272
PciI ACATGT 1 cut(s) 268
PfeI GAWTC 4 cut(s) 325, 539, 671, 708
PkrI GCNGC 3 cut(s) 161, 249, 499
PscI ACATGT 1 cut(s) 268
Psp6I CCWGG 2 cut(s) 346, 517
PspGI CCWGG 2 cut(s) 346, 517
PspN4I GGNNCC 2 cut(s) 411, 420
PsuI RGATCY 1 cut(s) 473
PvuII CAGCTG 1 cut(s) 430
RsaI GTAC 3 cut(s) 345, 360, 434
RsaNI GTAC 3 cut(s) 344, 359, 433
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 1 cut(s) 563
SatI GCNGC 3 cut(s) 160, 248, 498
Sau3AI GATC 6 cut(s) 47, 63, 228, 473, 613, 634
ScrFI CCNGG 2 cut(s) 348, 519
SduI GDGCHC 1 cut(s) 193
SfaNI GCATC 2 cut(s) 565, 634
SmiMI CAYNNNNRTG 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 3 cut(s) 35, 238, 525
SsiI CCGC 1 cut(s) 610
SspMI CTAG 3 cut(s) 372, 387, 757
StyD4I CCNGG 2 cut(s) 346, 517
TaaI ACNGT 2 cut(s) 403, 583
TaqI TCGA 2 cut(s) 66, 339
TasI AATT 3 cut(s) 35, 238, 525
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 4 cut(s) 325, 539, 671, 708
Tru1I TTAA 1 cut(s) 563
Tru9I TTAA 1 cut(s) 563
TscAI CASTG 2 cut(s) 654, 733
TseI GCWGC 3 cut(s) 159, 247, 497
TspDTI ATGAA 1 cut(s) 531
TspRI CASTG 2 cut(s) 654, 733
VneI GTGCAC 1 cut(s) 189
XagI CCTNNNNNAGG 1 cut(s) 564
XceI RCATGY 1 cut(s) 272
XspI CTAG 3 cut(s) 372, 387, 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.