Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000108

Physical Location & Seq

Genomic Coordinates Forward (+)

19496150 .. 19499600

Gene Structure 3451 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G016450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGCAACTACAAAAGCCAATCTTCCAGACCCATCTTCCACCACAACCAAGCTCACAGCTTTTTGCTCAGATCCATCTCCATTACCATAAACCTCACCTTCCCACAGTCTCTCTCTGCTTCCATAAACCCACATCAACAACACCCGAAAGCTCTACCAGTTCCAGCTGCATTCCAACAGAAACAGATAGGAGAACCAAACAGGGACATCTTTCTTCTTCTTCTTCATCTGGGTCTTGTAATTCATCGATTAATTCAATGATGGCGACAATGGTGGCGATCGATCCGTATGTTGTGTGGACGTTCTTTGCTTCTTTGTTGGGTTTTCTTCTTCTCTCCCTTCTCCGCCGACGTAATTACGGAAATGCCACCGCCGAGGAGAAGGGAATGGTTAAAAACAAGAACAAGACGACCGAGAATCATGTTTCTGAAGAATGTGTGAAGAGCTCCGGCAACGGAGACTTCCGGCAGGAGTTCGGCTCCGGAACTGACGTCATCATCGTTGGCGCCGGTGTTGCTGGCGCTGCTCTTGCACACACCCTTGGCAAGGAGGGAAGACTTGTTCGTGTGATTGAAAGAGACTTGACAGAGCCGGATCGAATTGTTGGCGAACTTCTCCAGCCTGGGGGATACCTGAAGCTGATTGACTTGGGACTTGAAGATTGTGTTGAGGAAATCGATGCTCAGCGAGTTCTCGGATATGCTCTTTTTAAGGATGGGAAGAATACTACACTGTCCTATCCCCTGGAACAGTTCCACTCAGATGTGGCGGGTAGAAGTTTTCACAATGGGCGATTCATACAGAGGATGAGAGAAAAAGCTGCTACTCTTCCCAATGTACAACTGGAGCAAGGTACAGTCACATCCCTGCTTGAAGAAAATGGGACAATTACGGGGGTTCAGTACAAAACTAAGGATGGTCAAGAACTTCAAGCTCATGCTCCTCTTACAATTGTCTGCGATGGTTGCTTCTCAAATCTGCGACGCACTCTTTGTAAGCCTCAGGTAGAAGTGCCCTCTTGTTTTGTGGGATTAATTTTGGAAAATTGTGAACTCCCATTTGCAAATCACGGGCATGTTATTCTTGCTGATCCATCTCCAATCCTGTTTTACCAAATCAGTAGTACAGAGGTCCGCTGTTTAGTTGATGTGCCTGGCCAGAAAGTACCTCCCATCACTAATGGAGCAATGGCTAACTATTTGAAGAATGTGGTAGCTCCACAGGTACTTAAACCACCTCGACAAAGCATGCCAGCCAATCCGCAGCCAACTCCTGGAGCCCTTTTAATGGGTGATGCTTTCAATATGCGTCATCCTTTAACTGGTGGAGGAATGACTGTGGCACTATCGGACATTGTTATACTCAGGGATCTTCTTAAACCATTACGTGACATAAATGATGCTTCTTCATTGTGCAACTACCTAGAATCCTTTTATACCTTGCGCAAGCCAGTTGCATCTACAATAAACACCTTGGCAGGTGCCCTATACAAGGTGTTCTCTGCCTCCCCTGATCAAGCAAGGAAGGAGATGTGCCAAGCATGCTTTGACTATCTGAGCCTTGGAGGTGCCTGTTCAACTGGACCAGTGGCTCTGCTCTCTGGTCTAAATCCTCGCCCGCTGAGTTTAGTTCTCCATTTTTTTGCTGTTGCGATATATGGTGTTGGACGTTTGCTTCTACCATTTCCTTCACCGAAACGCATGTGGATTGGAGTGAGATTAATCTGGAGCGCAGTAGGCATAATATTTCCCATCATCAAGGCAGAAGGTGTGAGACAGATGTTCTTTCCTGCTACTATTCCAGCTTGTCATAGGGCTCCTCCTGCTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004497 GO:0004506 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006629 GO:0006694 GO:0006950 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0012505 GO:0016125 GO:0016126 GO:0016491 GO:0016705 GO:0016709 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130

Pfam Domains

Protein Families

Protein Analysis

609

Amino Acids

66.38

Weight (kDa)

8.5

Isoelectric Point (pI)

48.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	162 - 333	8.2e-13	FAD binding domain
SE	PF08491	312 - 568	1.4e-103	Squalene epoxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000108)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22830 AT4G37760
fragaria_vesca	FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica	MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica	Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis	pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis	RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata	RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora	Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii	Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa	Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha	Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana	Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1466
AatII	GACGTC	1 cut(s)	492
Acc16I	TGCGCA	1 cut(s)	1442
Acc36I	ACCTGC	1 cut(s)	1466
AccB1I	GGYRCC	3 cut(s)	503, 1478, 1565
AccB7I	CCANNNNNTGG	1 cut(s)	1271
AccIII	TCCGGA	1 cut(s)	479
AciI	CCGC	6 cut(s)	343, 369, 767, 1132, 1259, 1616
AclWI	GGATC	5 cut(s)	64, 275, 600, 1082, 1374
AcoI	YGGCCR	1 cut(s)	1153
AcuI	CTGAAG	2 cut(s)	447, 653
AcyI	GRCGYC	2 cut(s)	489, 504
AfaI	GTAC	6 cut(s)	837, 853, 902, 1123, 1164, 1224
AfiI	CCNNNNNNNGG	6 cut(s)	544, 622, 1271, 1285, 1319, 1489
AgsI	TTSAA	8 cut(s)	255, 572, 656, 872, 929, 1201, 1300, 1575
AjnI	CCWGG	4 cut(s)	619, 741, 1150, 1270
AloI	GAACNNNNNNTCC	2 cut(s)	543, 575
Alw21I	GWGCWC	1 cut(s)	446
Alw26I	GTCTC	4 cut(s)	112, 450, 570, 1765
AlwI	GGATC	5 cut(s)	64, 275, 600, 1082, 1374
Aor13HI	TCCGGA	1 cut(s)	479
AoxI	GGCC	1 cut(s)	1153
ApeKI	GCWGC	4 cut(s)	165, 521, 818, 1261
ArsI	GACNNNNNNTTYG	2 cut(s)	972, 1004
AseI	ATTAAT	3 cut(s)	249, 1031, 1718
AspLEI	GCGC	4 cut(s)	506, 521, 1443, 1730
AspS9I	GGNCC	2 cut(s)	1129, 1580
AsuHPI	GGTGA	3 cut(s)	86, 1301, 1680
AvaII	GGWCC	2 cut(s)	1129, 1580
AxyI	CCTNAGG	1 cut(s)	999
BaeGI	GKGCMC	2 cut(s)	1014, 1483
BalI	TGGCCA	1 cut(s)	1155
BanI	GGYRCC	3 cut(s)	503, 1478, 1565
BanII	GRGCYC	3 cut(s)	446, 1279, 1816
BbsI	GAAGAC	1 cut(s)	559
Bbv12I	GWGCWC	1 cut(s)	446
BbvI	GCAGC	4 cut(s)	152, 508, 805, 1273
BccI	CCATC	9 cut(s)	39, 81, 253, 707, 908, 953, 1099, 1178, 1757
BciT130I	CCWGG	4 cut(s)	621, 743, 1152, 1272
BciVI	GTATCC	1 cut(s)	620
BclI	TGATCA	1 cut(s)	1510
BcoDI	GTCTC	4 cut(s)	112, 450, 570, 1765
BfaI	CTAG	1 cut(s)	1421
BfoI	RGCGCY	2 cut(s)	507, 522
BfuAI	ACCTGC	1 cut(s)	1466
BfuI	GTATCC	1 cut(s)	620
BisI	GCNGC	4 cut(s)	166, 522, 819, 1262
BlpI	GCTNAGC	1 cut(s)	681
BlsI	GCNGC	4 cut(s)	167, 523, 820, 1263
Bme1390I	CCNGG	4 cut(s)	621, 743, 1152, 1272
Bme18I	GGWCC	2 cut(s)	1129, 1580
BmgT120I	GGNCC	2 cut(s)	1129, 1580
BmiI	GGNNCC	6 cut(s)	478, 505, 1276, 1480, 1567, 1815
BmrFI	CCNGG	4 cut(s)	621, 743, 1152, 1272
BmsI	GCATC	4 cut(s)	667, 1282, 1387, 1463
BpiI	GAAGAC	1 cut(s)	559
BplI	GAGNNNNNCTC	2 cut(s)	461, 493
BpmI	CTGGAG	4 cut(s)	599, 863, 1293, 1744
Bpu1102I	GCTNAGC	1 cut(s)	681
Bsa29I	ATCGAT	3 cut(s)	245, 279, 675
BsaAI	YACGTR	1 cut(s)	1385
BsaHI	GRCGYC	2 cut(s)	489, 504
BsaJI	CCNNGG	6 cut(s)	372, 538, 620, 741, 1470, 1558
BsaWI	WCCGGW	1 cut(s)	479
Bsc4I	CCNNNNNNNGG	6 cut(s)	544, 622, 1271, 1285, 1319, 1489
Bse118I	RCCGGY	1 cut(s)	506
Bse1I	ACTGG	6 cut(s)	156, 846, 1324, 1448, 1582, 1583
Bse21I	CCTNAGG	1 cut(s)	999
Bse3DI	GCAATG	1 cut(s)	1191
BseAI	TCCGGA	1 cut(s)	479
BseBI	CCWGG	4 cut(s)	621, 743, 1152, 1272
BseCI	ATCGAT	3 cut(s)	245, 279, 675
BseDI	CCNNGG	6 cut(s)	372, 538, 620, 741, 1470, 1558
BseGI	GGATG	5 cut(s)	718, 810, 860, 919, 1309
BseLI	CCNNNNNNNGG	6 cut(s)	544, 622, 1271, 1285, 1319, 1489
BseMI	GCAATG	1 cut(s)	1191
BseMII	CTCAG	7 cut(s)	80, 695, 771, 1013, 1375, 1544, 1610
BseNI	ACTGG	6 cut(s)	156, 846, 1324, 1448, 1582, 1583
BseRI	GAGGAG	3 cut(s)	389, 930, 1806
BseSI	GKGCMC	2 cut(s)	1014, 1483
BseXI	GCAGC	4 cut(s)	152, 508, 805, 1273
Bsh1285I	CGRYCG	2 cut(s)	279, 411
BshFI	GGCC	1 cut(s)	1155
BshNI	GGYRCC	3 cut(s)	503, 1478, 1565
BshVI	ATCGAT	3 cut(s)	245, 279, 675
BsiEI	CGRYCG	2 cut(s)	279, 411
BsiHKAI	GWGCWC	1 cut(s)	446
BsiSI	CCGG	5 cut(s)	447, 463, 480, 507, 590
BslFI	GGGAC	3 cut(s)	216, 663, 895
BslI	CCNNNNNNNGG	6 cut(s)	544, 622, 1271, 1285, 1319, 1489
BsmAI	GTCTC	4 cut(s)	112, 450, 570, 1765
BsmFI	GGGAC	3 cut(s)	216, 663, 895
BsmI	GAATGC	1 cut(s)	168
BsnI	GGCC	1 cut(s)	1155
Bsp1286I	GDGCHC	5 cut(s)	446, 1014, 1279, 1483, 1816
Bsp13I	TCCGGA	1 cut(s)	479
Bsp1407I	TGTACA	1 cut(s)	835
Bsp143I	GATC	7 cut(s)	69, 276, 280, 592, 1087, 1366, 1510
Bsp1720I	GCTNAGC	1 cut(s)	681
BspACI	CCGC	6 cut(s)	343, 369, 767, 1132, 1259, 1616
BspANI	GGCC	1 cut(s)	1155
BspCNI	CTCAG	7 cut(s)	79, 694, 770, 1012, 1374, 1545, 1611
BspDI	ATCGAT	3 cut(s)	245, 279, 675
BspEI	TCCGGA	1 cut(s)	479
BspLI	GGNNCC	6 cut(s)	478, 505, 1276, 1480, 1567, 1815
BspMI	ACCTGC	1 cut(s)	1466
BspPI	GGATC	5 cut(s)	64, 275, 600, 1082, 1374
BspQI	GCTCTTC	1 cut(s)	434
BspT107I	GGYRCC	3 cut(s)	503, 1478, 1565
BsrDI	GCAATG	1 cut(s)	1191
BsrFI	RCCGGY	1 cut(s)	506
BsrGI	TGTACA	1 cut(s)	835
BsrI	ACTGG	6 cut(s)	156, 846, 1324, 1448, 1582, 1583
BssAI	RCCGGY	1 cut(s)	506
BssECI	CCNNGG	6 cut(s)	372, 538, 620, 741, 1470, 1558
BssMI	GATC	7 cut(s)	69, 276, 280, 592, 1087, 1366, 1510
BssNI	GRCGYC	2 cut(s)	489, 504
BssT1I	CCWWGG	3 cut(s)	538, 1470, 1558
Bst2UI	CCWGG	4 cut(s)	621, 743, 1152, 1272
Bst4CI	ACNGT	5 cut(s)	106, 732, 750, 856, 1336
Bst6I	CTCTTC	2 cut(s)	434, 831
BstACI	GRCGYC	2 cut(s)	489, 504
BstAUI	TGTACA	1 cut(s)	835
BstBAI	YACGTR	1 cut(s)	1385
BstC8I	GCNNGC	6 cut(s)	517, 1247, 1251, 1445, 1540, 1616
BstDEI	CTNAG	8 cut(s)	66, 681, 757, 909, 999, 1361, 1553, 1619
BstENI	CCTNNNNNAGG	2 cut(s)	542, 1487
BstF5I	GGATG	5 cut(s)	718, 810, 860, 919, 1309
BstH2I	RGCGCY	2 cut(s)	507, 522
BstHHI	GCGC	4 cut(s)	506, 521, 1443, 1730
BstKTI	GATC	7 cut(s)	72, 279, 283, 595, 1090, 1369, 1513
BstMAI	GTCTC	4 cut(s)	112, 450, 570, 1765
BstMBI	GATC	7 cut(s)	69, 276, 280, 592, 1087, 1366, 1510
BstMCI	CGRYCG	2 cut(s)	279, 411
BstMWI	GCNNNNNNNGC	7 cut(s)	512, 521, 527, 963, 1539, 1734, 1820
BstNI	CCWGG	4 cut(s)	621, 743, 1152, 1272
BstNSI	RCATGY	4 cut(s)	1076, 1249, 1542, 1702
BstSCI	CCNGG	4 cut(s)	619, 741, 1150, 1270
BstSLI	GKGCMC	2 cut(s)	1014, 1483
BstV1I	GCAGC	4 cut(s)	152, 508, 805, 1273
BstV2I	GAAGAC	1 cut(s)	559
BstX2I	RGATCY	2 cut(s)	69, 1366
BstYI	RGATCY	2 cut(s)	69, 1366
Bsu15I	ATCGAT	3 cut(s)	245, 279, 675
Bsu36I	CCTNAGG	1 cut(s)	999
BsuI	GTATCC	1 cut(s)	620
BsuRI	GGCC	1 cut(s)	1155
BsuTUI	ATCGAT	3 cut(s)	245, 279, 675
BtgZI	GCGATG	1 cut(s)	972
BtsCI	GGATG	5 cut(s)	718, 810, 860, 919, 1309
BtsIMutI	CAGTG	2 cut(s)	728, 1590
BveI	ACCTGC	1 cut(s)	1466
Cac8I	GCNNGC	6 cut(s)	517, 1247, 1251, 1445, 1540, 1616
CfoI	GCGC	4 cut(s)	506, 521, 1443, 1730
Cfr10I	RCCGGY	1 cut(s)	506
Cfr13I	GGNCC	2 cut(s)	1129, 1580
ClaI	ATCGAT	3 cut(s)	245, 279, 675
CseI	GACGC	2 cut(s)	990, 1295
Csp6I	GTAC	6 cut(s)	836, 852, 901, 1122, 1163, 1223
CviAII	CATG	6 cut(s)	419, 935, 1073, 1246, 1539, 1699
CviQI	GTAC	6 cut(s)	836, 852, 901, 1122, 1163, 1223
DdeI	CTNAG	8 cut(s)	66, 681, 757, 909, 999, 1361, 1553, 1619
DinI	GGCGCC	1 cut(s)	505
DpnI	GATC	7 cut(s)	71, 278, 282, 594, 1089, 1368, 1512
DpnII	GATC	7 cut(s)	69, 276, 280, 592, 1087, 1366, 1510
EaeI	YGGCCR	1 cut(s)	1153
Eam1104I	CTCTTC	2 cut(s)	434, 831
EarI	CTCTTC	2 cut(s)	434, 831
EciI	GGCGGA	1 cut(s)	332
Ecl136II	GAGCTC	1 cut(s)	444
Eco130I	CCWWGG	3 cut(s)	538, 1470, 1558
Eco24I	GRGCYC	3 cut(s)	446, 1279, 1816
Eco47I	GGWCC	2 cut(s)	1129, 1580
Eco53kI	GAGCTC	1 cut(s)	444
Eco57I	CTGAAG	2 cut(s)	447, 653
Eco81I	CCTNAGG	1 cut(s)	999
EcoICRI	GAGCTC	1 cut(s)	444
EcoNI	CCTNNNNNAGG	2 cut(s)	542, 1487
EcoRII	CCWGG	4 cut(s)	619, 741, 1150, 1270
EcoT14I	CCWWGG	3 cut(s)	538, 1470, 1558
EcoT38I	GRGCYC	3 cut(s)	446, 1279, 1816
EgeI	GGCGCC	1 cut(s)	505
EheI	GGCGCC	1 cut(s)	505
ErhI	CCWWGG	3 cut(s)	538, 1470, 1558
FaeI	CATG	6 cut(s)	422, 938, 1076, 1249, 1542, 1702
FalI	AAGNNNNNCTT	1 cut(s)	37
FaqI	GGGAC	3 cut(s)	216, 663, 895
FatI	CATG	6 cut(s)	418, 934, 1072, 1245, 1538, 1698
FauI	CCCGC	2 cut(s)	760, 1623
FbaI	TGATCA	1 cut(s)	1510
Fnu4HI	GCNGC	4 cut(s)	166, 522, 819, 1262
FokI	GGATG	5 cut(s)	725, 817, 847, 926, 1296
FriOI	GRGCYC	3 cut(s)	446, 1279, 1816
Fsp4HI	GCNGC	4 cut(s)	166, 522, 819, 1262
FspBI	CTAG	1 cut(s)	1421
FspI	TGCGCA	1 cut(s)	1442
GlaI	GCGC	4 cut(s)	505, 520, 1442, 1729
GluI	GCNGC	4 cut(s)	166, 522, 819, 1262
GsuI	CTGGAG	4 cut(s)	599, 863, 1293, 1744
HaeII	RGCGCY	2 cut(s)	507, 522
HaeIII	GGCC	1 cut(s)	1155
HapII	CCGG	5 cut(s)	447, 463, 480, 507, 590
HgaI	GACGC	2 cut(s)	990, 1295
HhaI	GCGC	4 cut(s)	506, 521, 1443, 1730
Hin1I	GRCGYC	2 cut(s)	489, 504
Hin1II	CATG	6 cut(s)	422, 938, 1076, 1249, 1542, 1702
Hin6I	GCGC	4 cut(s)	504, 519, 1441, 1728
HinP1I	GCGC	4 cut(s)	504, 519, 1441, 1728
HinfI	GANTC	3 cut(s)	415, 792, 1424
HpaII	CCGG	5 cut(s)	447, 463, 480, 507, 590
HphI	GGTGA	3 cut(s)	86, 1301, 1680
Hpy166II	GTNNAC	2 cut(s)	297, 1049
Hpy188I	TCNGA	6 cut(s)	69, 427, 695, 760, 1348, 1554
Hpy188III	TCNNGA	4 cut(s)	25, 480, 920, 1723
Hpy8I	GTNNAC	2 cut(s)	297, 1049
Hpy99I	CGWCG	2 cut(s)	351, 984
HpyAV	CCTTC	6 cut(s)	107, 347, 373, 1516, 1695, 1757
HpyCH4III	ACNGT	5 cut(s)	106, 732, 750, 856, 1336
HpyCH4IV	ACGT	5 cut(s)	299, 349, 489, 1384, 1666
HpyCH4V	TGCA	6 cut(s)	4, 168, 530, 1061, 1413, 1454
HpyF10VI	GCNNNNNNNGC	7 cut(s)	512, 521, 527, 963, 1539, 1734, 1820
HpyF3I	CTNAG	8 cut(s)	66, 681, 757, 909, 999, 1361, 1553, 1619
HpySE526I	ACGT	5 cut(s)	299, 349, 489, 1384, 1666
Hsp92I	GRCGYC	2 cut(s)	489, 504
Hsp92II	CATG	6 cut(s)	422, 938, 1076, 1249, 1542, 1702
HspAI	GCGC	4 cut(s)	504, 519, 1441, 1728
KasI	GGCGCC	1 cut(s)	503
Kpn2I	TCCGGA	1 cut(s)	479
Ksp22I	TGATCA	1 cut(s)	1510
Kzo9I	GATC	7 cut(s)	69, 276, 280, 592, 1087, 1366, 1510
LguI	GCTCTTC	1 cut(s)	434
LmnI	GCTCC	9 cut(s)	449, 482, 844, 943, 1181, 1219, 1274, 1725, 1819
Lsp1109I	GCAGC	4 cut(s)	152, 508, 805, 1273
LweI	GCATC	4 cut(s)	667, 1282, 1387, 1463
MaeI	CTAG	1 cut(s)	1421
MaeII	ACGT	5 cut(s)	299, 349, 489, 1384, 1666
MaeIII	GTNAC	2 cut(s)	856, 1385
MalI	GATC	7 cut(s)	71, 278, 282, 594, 1089, 1368, 1512
MboI	GATC	7 cut(s)	69, 276, 280, 592, 1087, 1366, 1510
MfeI	CAATTG	1 cut(s)	948
MflI	RGATCY	2 cut(s)	69, 1366
MhlI	GDGCHC	5 cut(s)	446, 1014, 1279, 1483, 1816
MlsI	TGGCCA	1 cut(s)	1155
MluCI	AATT	8 cut(s)	238, 250, 352, 597, 885, 948, 1032, 1042
MluNI	TGGCCA	1 cut(s)	1155
Mly113I	GGCGCC	1 cut(s)	504
MmeI	TCCRAC	2 cut(s)	197, 1642
Mox20I	TGGCCA	1 cut(s)	1155
MroI	TCCGGA	1 cut(s)	479
MscI	TGGCCA	1 cut(s)	1155
MseI	TTAA	9 cut(s)	249, 390, 708, 1031, 1227, 1283, 1316, 1374, 1718
MslI	CAYNNNNRTG	2 cut(s)	759, 1071
Msp20I	TGGCCA	1 cut(s)	1155
MspA1I	CMGCKG	3 cut(s)	165, 1134, 1618
MspI	CCGG	5 cut(s)	447, 463, 480, 507, 590
MspR9I	CCNGG	4 cut(s)	621, 743, 1152, 1272
MunI	CAATTG	1 cut(s)	948
Mva1269I	GAATGC	1 cut(s)	168
MvaI	CCWGG	4 cut(s)	621, 743, 1152, 1272
MwoI	GCNNNNNNNGC	7 cut(s)	512, 521, 527, 963, 1539, 1734, 1820
NarI	GGCGCC	1 cut(s)	504
NdeII	GATC	7 cut(s)	69, 276, 280, 592, 1087, 1366, 1510
NlaIII	CATG	6 cut(s)	422, 938, 1076, 1249, 1542, 1702
NlaIV	GGNNCC	6 cut(s)	478, 505, 1276, 1480, 1567, 1815
NmeAIII	GCCGAG	1 cut(s)	397
NmuCI	GTSAC	2 cut(s)	856, 1385
NsbI	TGCGCA	1 cut(s)	1442
NspI	RCATGY	4 cut(s)	1076, 1249, 1542, 1702
PaeI	GCATGC	2 cut(s)	1249, 1542
PaqCI	CACCTGC	1 cut(s)	1466
PciSI	GCTCTTC	1 cut(s)	434
PcsI	WCGNNNNNNNCGW	1 cut(s)	495
PctI	GAATGC	1 cut(s)	168
PfeI	GAWTC	3 cut(s)	415, 792, 1424
PflMI	CCANNNNNTGG	1 cut(s)	1271
PfoI	TCCNGGA	1 cut(s)	1270
PkrI	GCNGC	4 cut(s)	167, 523, 820, 1263
Ple19I	CGATCG	1 cut(s)	279
PluTI	GGCGCC	1 cut(s)	507
Ppu21I	YACGTR	1 cut(s)	1385
PshBI	ATTAAT	3 cut(s)	249, 1031, 1718
Psp124BI	GAGCTC	1 cut(s)	446
Psp6I	CCWGG	4 cut(s)	619, 741, 1150, 1270
PspGI	CCWGG	4 cut(s)	619, 741, 1150, 1270
PspN4I	GGNNCC	6 cut(s)	478, 505, 1276, 1480, 1567, 1815
PspPI	GGNCC	2 cut(s)	1129, 1580
PsuI	RGATCY	2 cut(s)	69, 1366
PvuI	CGATCG	1 cut(s)	279
PvuII	CAGCTG	1 cut(s)	165
RsaI	GTAC	6 cut(s)	837, 853, 902, 1123, 1164, 1224
RsaNI	GTAC	6 cut(s)	836, 852, 901, 1122, 1163, 1223
RseI	CAYNNNNRTG	2 cut(s)	759, 1071
SacI	GAGCTC	1 cut(s)	446
SapI	GCTCTTC	1 cut(s)	434
SaqAI	TTAA	9 cut(s)	249, 390, 708, 1031, 1227, 1283, 1316, 1374, 1718
SatI	GCNGC	4 cut(s)	166, 522, 819, 1262
Sau3AI	GATC	7 cut(s)	69, 276, 280, 592, 1087, 1366, 1510
Sau96I	GGNCC	2 cut(s)	1129, 1580
ScrFI	CCNGG	4 cut(s)	621, 743, 1152, 1272
SduI	GDGCHC	5 cut(s)	446, 1014, 1279, 1483, 1816
SfaNI	GCATC	4 cut(s)	667, 1282, 1387, 1463
SfoI	GGCGCC	1 cut(s)	505
SgrAI	CRCCGGYG	1 cut(s)	506
SinI	GGWCC	2 cut(s)	1129, 1580
SmiMI	CAYNNNNRTG	2 cut(s)	759, 1071
SphI	GCATGC	2 cut(s)	1249, 1542
Sse9I	AATT	8 cut(s)	238, 250, 352, 597, 885, 948, 1032, 1042
SsiI	CCGC	6 cut(s)	343, 369, 767, 1132, 1259, 1616
SspDI	GGCGCC	1 cut(s)	503
SspI	AATATT	1 cut(s)	1743
SspMI	CTAG	1 cut(s)	1421
SstI	GAGCTC	1 cut(s)	446
StyD4I	CCNGG	4 cut(s)	619, 741, 1150, 1270
StyI	CCWWGG	3 cut(s)	538, 1470, 1558
TaaI	ACNGT	5 cut(s)	106, 732, 750, 856, 1336
TaiI	ACGT	5 cut(s)	302, 352, 492, 1387, 1669
TaqI	TCGA	5 cut(s)	245, 279, 595, 675, 1237
TaqII	GACCGA	1 cut(s)	425
TasI	AATT	8 cut(s)	238, 250, 352, 597, 885, 948, 1032, 1042
TatI	WGTACW	3 cut(s)	835, 900, 1121
TfiI	GAWTC	3 cut(s)	415, 792, 1424
Tru1I	TTAA	9 cut(s)	249, 390, 708, 1031, 1227, 1283, 1316, 1374, 1718
Tru9I	TTAA	9 cut(s)	249, 390, 708, 1031, 1227, 1283, 1316, 1374, 1718
TscAI	CASTG	2 cut(s)	735, 1590
TseFI	GTSAC	2 cut(s)	856, 1385
TseI	GCWGC	4 cut(s)	165, 521, 818, 1261
Tsp45I	GTSAC	2 cut(s)	856, 1385
TspDTI	ATGAA	4 cut(s)	213, 231, 784, 1395
TspGWI	ACGGA	3 cut(s)	273, 372, 468
TspRI	CASTG	2 cut(s)	735, 1590
Van91I	CCANNNNNTGG	1 cut(s)	1271
VpaK11BI	GGWCC	2 cut(s)	1129, 1580
VspI	ATTAAT	3 cut(s)	249, 1031, 1718
XagI	CCTNNNNNAGG	2 cut(s)	542, 1487
XceI	RCATGY	4 cut(s)	1076, 1249, 1542, 1702
XcmI	CCANNNNNNNNNTGG	1 cut(s)	838
XspI	CTAG	1 cut(s)	1421
ZraI	GACGTC	1 cut(s)	490

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.