RchiOBHm_Chr4g0411491

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000108
Physical Location & Seq
Forward (+)
35338365 .. 35339575
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38229

Sequence Viewer

Length: 696 bp
ATGGGCAAGTATTTGAAATCTGTGGTGGCTCCTCAGATTCCCCCACAGCTGCACAATGCTTTTATGGCAGCAGTAGAGGAAGGAAACATCAGAACAATGCAAAACAAAAGCATGGCTGCTAATCCTGTTCCCACTCCTGGTGCAATTTTATTGGGGGATGCATTCAACATGAGGCATCCATTAACAGGAGGAGGAATGACTGTGGCTCTTTCAGACATTGTTCTTCTCCGTAATCTTCTTAGACCCCTACGTGATCTCAGTGACGCAACTGCTTTGTGCAATTACCTTGAAGCATTCTACACACTTCGTAAGCCTGTGTCATCTACCATAAACACATTGGCAGGTGCTTTATACAAGGTGTTTTGTGCATCTCCTGATCCAGCAAAACAGGAAATGCGTGAAGCATGTTTTGACTATCTTAGCCTTGGAGGTATCTGTTCATATGGACCAGTATCTCTGCTCTCTGGTCTTAACCCTCGTCCCCTGCACTTATTTCTCCATTTCTTTGCCGTTGCCATCTATGGCGTCGGACGCTTAATGCTGCCCTTCCCTTCACCTCAGCGCATGTGGCTTGGGGCTAGATTGATCTTGAGTGCATCAAGCATCATATTCCCGATTATTAAGGGGGAAGGAGTTAGACAGATGTTTTTTCCTGCAACAGTGCCAGCATTTTACAGATCTCCTCCTCTTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

231

Amino Acids

25.22

Weight (kDa)

9.54

Isoelectric Point (pI)

50.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SE PF08491 1 - 190 4.4e-78 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 332
AclWI GGATC 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 525
AfiI CCNNNNNNNGG 2 cut(s) 137, 185
AgsI TTSAA 3 cut(s) 16, 166, 290
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 1 cut(s) 49
AluI AGCT 1 cut(s) 49
AlwI GGATC 1 cut(s) 371
ApeKI GCWGC 4 cut(s) 49, 68, 116, 541
AspLEI GCGC 1 cut(s) 564
AspS9I GGNCC 1 cut(s) 446
AsuHPI GGTGA 1 cut(s) 546
AvaII GGWCC 1 cut(s) 446
BbvCI CCTCAGC 1 cut(s) 558
BbvI GCAGC 4 cut(s) 36, 80, 103, 528
BccI CCATC 1 cut(s) 524
BceAI ACGGC 1 cut(s) 494
BciT130I CCWGG 1 cut(s) 138
BfaI CTAG 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 332
BglII AGATCT 1 cut(s) 677
BisI GCNGC 4 cut(s) 50, 69, 117, 542
BlsI GCNGC 4 cut(s) 51, 70, 118, 543
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 446
BmgT120I GGNCC 1 cut(s) 446
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 5 cut(s) 148, 184, 377, 605, 612
Bpu10I CCTNAGC 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 610
BsaAI YACGTR 1 cut(s) 251
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 424
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 185
Bse1I ACTGG 1 cut(s) 449
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 2 cut(s) 163, 175
BseLI CCNNNNNNNGG 2 cut(s) 137, 185
BseMII CTCAG 3 cut(s) 47, 271, 572
BseNI ACTGG 1 cut(s) 449
BseRI GAGGAG 4 cut(s) 21, 204, 672, 675
BseXI GCAGC 4 cut(s) 36, 80, 103, 528
BsgI GTGCAG 2 cut(s) 35, 470
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 2 cut(s) 137, 185
BsmFI GGGAC 1 cut(s) 465
BsmI GAATGC 2 cut(s) 161, 293
Bsp143I GATC 4 cut(s) 253, 376, 585, 677
BspCNI CTCAG 3 cut(s) 46, 270, 571
BspLI GGNNCC 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 332
BspPI GGATC 1 cut(s) 371
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 4 cut(s) 253, 376, 585, 677
BssNI GRCGYC 1 cut(s) 525
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 202, 661
Bst6I CTCTTC 1 cut(s) 693
BstACI GRCGYC 1 cut(s) 525
BstBAI YACGTR 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 666
BstDEI CTNAG 5 cut(s) 33, 239, 257, 419, 558
BstF5I GGATG 2 cut(s) 163, 175
BstHHI GCGC 1 cut(s) 564
BstKTI GATC 4 cut(s) 256, 379, 588, 680
BstMBI GATC 4 cut(s) 253, 376, 585, 677
BstMWI GCNNNNNNNGC 3 cut(s) 65, 531, 568
BstNI CCWGG 1 cut(s) 138
BstNSI RCATGY 2 cut(s) 408, 568
BstSCI CCNGG 1 cut(s) 136
BstV1I GCAGC 4 cut(s) 36, 80, 103, 528
BstX2I RGATCY 1 cut(s) 677
BstYI RGATCY 1 cut(s) 677
BtsCI GGATG 2 cut(s) 163, 175
BtsIMutI CAGTG 3 cut(s) 265, 666, 691
BveI ACCTGC 1 cut(s) 332
Cac8I GCNNGC 1 cut(s) 666
CfoI GCGC 1 cut(s) 564
Cfr13I GGNCC 1 cut(s) 446
CseI GACGC 3 cut(s) 272, 514, 540
CviAII CATG 4 cut(s) 112, 169, 405, 565
CviJI RGCY 8 cut(s) 29, 49, 116, 206, 313, 423, 571, 578
CviKI_1 RGCY 8 cut(s) 29, 49, 116, 206, 313, 423, 571, 578
DdeI CTNAG 5 cut(s) 33, 239, 257, 419, 558
DpnI GATC 4 cut(s) 255, 378, 587, 679
DpnII GATC 4 cut(s) 253, 376, 585, 677
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
Eco130I CCWWGG 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 424
FaeI CATG 4 cut(s) 115, 172, 408, 568
FaqI GGGAC 1 cut(s) 465
FatI CATG 4 cut(s) 111, 168, 404, 564
FauNDI CATATG 1 cut(s) 442
Fnu4HI GCNGC 4 cut(s) 50, 69, 117, 542
FokI GGATG 2 cut(s) 162, 170
Fsp4HI GCNGC 4 cut(s) 50, 69, 117, 542
FspBI CTAG 1 cut(s) 579
GlaI GCGC 1 cut(s) 563
GluI GCNGC 4 cut(s) 50, 69, 117, 542
HgaI GACGC 3 cut(s) 272, 514, 540
HhaI GCGC 1 cut(s) 564
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 4 cut(s) 115, 172, 408, 568
Hin6I GCGC 1 cut(s) 562
HinP1I GCGC 1 cut(s) 562
HinfI GANTC 1 cut(s) 37
HphI GGTGA 1 cut(s) 546
Hpy188I TCNGA 4 cut(s) 36, 92, 214, 530
Hpy188III TCNNGA 3 cut(s) 374, 589, 613
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 4 cut(s) 74, 556, 561, 623
HpyCH4III ACNGT 2 cut(s) 202, 661
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 9 cut(s) 52, 100, 143, 161, 279, 368, 487, 596, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 531, 568
HpyF3I CTNAG 5 cut(s) 33, 239, 257, 419, 558
HpySE526I ACGT 1 cut(s) 250
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 4 cut(s) 115, 172, 408, 568
HspAI GCGC 1 cut(s) 562
Kzo9I GATC 4 cut(s) 253, 376, 585, 677
LmnI GCTCC 1 cut(s) 34
Lsp1109I GCAGC 4 cut(s) 36, 80, 103, 528
LweI GCATC 5 cut(s) 148, 184, 377, 605, 612
MaeI CTAG 1 cut(s) 579
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 1 cut(s) 260
MalI GATC 4 cut(s) 255, 378, 587, 679
MboI GATC 4 cut(s) 253, 376, 585, 677
MboII GAAGA 3 cut(s) 215, 227, 680
MflI RGATCY 1 cut(s) 677
MluCI AATT 2 cut(s) 144, 280
MmeI TCCRAC 1 cut(s) 508
Mph1103I ATGCAT 1 cut(s) 163
MseI TTAA 4 cut(s) 182, 471, 536, 621
MspA1I CMGCKG 1 cut(s) 49
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 2 cut(s) 161, 293
MvaI CCWGG 1 cut(s) 138
MwoI GCNNNNNNNGC 3 cut(s) 65, 531, 568
NdeI CATATG 1 cut(s) 442
NdeII GATC 4 cut(s) 253, 376, 585, 677
NlaIII CATG 4 cut(s) 115, 172, 408, 568
NlaIV GGNNCC 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 260
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 2 cut(s) 408, 568
PaqCI CACCTGC 1 cut(s) 332
PctI GAATGC 2 cut(s) 161, 293
PfeI GAWTC 1 cut(s) 37
PkrI GCNGC 4 cut(s) 51, 70, 118, 543
Ppu21I YACGTR 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 30
PspPI GGNCC 1 cut(s) 446
PsuI RGATCY 1 cut(s) 677
PvuII CAGCTG 1 cut(s) 49
SaqAI TTAA 4 cut(s) 182, 471, 536, 621
SatI GCNGC 4 cut(s) 50, 69, 117, 542
Sau3AI GATC 4 cut(s) 253, 376, 585, 677
Sau96I GGNCC 1 cut(s) 446
ScrFI CCNGG 1 cut(s) 138
SetI ASST 7 cut(s) 51, 253, 288, 346, 360, 433, 559
SfaNI GCATC 5 cut(s) 148, 184, 377, 605, 612
SinI GGWCC 1 cut(s) 446
SmlI CTYRAG 1 cut(s) 589
SmoI CTYRAG 1 cut(s) 589
Sse9I AATT 2 cut(s) 144, 280
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 2 cut(s) 202, 661
TaiI ACGT 1 cut(s) 253
TasI AATT 2 cut(s) 144, 280
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 4 cut(s) 182, 471, 536, 621
Tru9I TTAA 4 cut(s) 182, 471, 536, 621
TscAI CASTG 2 cut(s) 265, 666
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 4 cut(s) 49, 68, 116, 541
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 429
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 2 cut(s) 265, 666
VpaK11BI GGWCC 1 cut(s) 446
XceI RCATGY 2 cut(s) 408, 568
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XspI CTAG 1 cut(s) 579
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.