Rroxscaffold_7G00214440

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000108
Physical Location & Seq
Reverse (-)
65094830 .. 65098255
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214440.1

Sequence Viewer

Length: 1578 bp
ATGGTTTCTTACGAGTATGTTTTGGCTGGGGTGTTGGTTTCTTTGCTGGGCTCTATTTTCTTCATGATCATCAACTCTACTTTGGGAGGAAAGAAACAATTCAAGGCTGCAGGTGCACTAAAGAGTAATGGGATCGGAAACGGTGAGTTTCTGCCGGAAATGGAGGAGGAGAAAAATATCGATGTCGTCATTGTCGGTGCCGGAGTTTCTGGTGCAGCTCTTGCTTACACTCTTGCCAAGGAAGGACGGCGTGTGCATGTAATTGAAAGAGACTTGAGTGAGCCAGACAGAATTGTTGGGGAGTTGTTGCAGCCTGGGGGATATCTCAAATTGATTGAGTTGGGTCTAGAAGATTGTGCAAATGAGTCGATTGATGCCCAGAAAGTGTTTGGTTATGCTCTTTACAATGATGGGAAGGATACAACACTATCTTATCCCTTGGAGAAATACAGTTCAGATGTGGCCGGGAGGAGTTTCCACAATGGGCGTTTCATTCAAAGAATGCGTGAAAAAGCTACCACTCTTTCCAATGTGAAAATGGAACAAGGATCAGTGACAACACTAATAGAGGAAAAGGGCATTGTCAAAGGGGTGATTTACAAGAACAAGGCTGGAGAGGAGATGAGAACATATGCTCCACTAACAATCGTGTGCGATGGCTGCTTTTCAAATTTGCGCAAATCTCTTAGTGCTCCAAAGGTTGAAAGTCCCTCTTGTTTTGTGGGTTTGATCTTGGAGGACTGTGAGCTTCCACACCCAAATCATGGGCATGTCATTCTGGGAGACCCTTCACCCATCCTGTTTTATCCCATCAGTTCCACCGAGGTTCGCTGTTTGGTAGATGTGCCTGGAACAAAACTACCTTCAGTAGCTAGTGGTGAAATGGCCAACTATTTGAAAACTGTGGTAGCGCCTCAGGTTCCCCCACAACTGTACAATGCTTTTATGGCCGCTGTCAACAAGGGAAACATCAGATCCATGCAAAACAGAAGCATGGCTGCAAATCCTATTCCCACTCCTGGCGCAATTTTGTTGGGGGATTCATTCAACATGAGGCATCCTTTAACAGGAGGAGGAATGACCGTGGCTCTTTCAGACATTGTTCTTCTCCGGGATCTTCTTAGACCCCTACATAATCTCAATGATGCACCTGCTTTGTGCAATTACCTTGAATCATTTTACACACTGCGTAAGCCTGTGTCATCTACCATAAACACATTGGCAGGTGCATTGTACAAGGTGTTTTGTGCATCACCTGATCCAGCAAGACAGGAAATGCGTGAAGCCTGTTTTGGCTATTTGAGCCTTGGAGGCATCTGCTCTTATGGACCAGTATCTCTTCTCTCTGGTCTTAACCCTCGTCCATTACACTTGTTTCTCCATTTCTTTGCAGTTGCCATCTATGGTGTTGGCCGCTTAATGCTTCCGTTCCCTTCACCTAAACGCATGTGGCTTGGTTTTCGATTGATCTTGAGTGCGTCAGGCATCATATTCCCGATTATAAAGAGTGAAGGAATTAGACAGATGTTCTTTCCTGCCACAATACCAGCATATTACAGATCTCCTCCTGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

525

Amino Acids

57.18

Weight (kDa)

8.39

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 60 - 372 5.5e-17 FAD binding domain
DAO PF01266 61 - 91 7.7e-08 FAD dependent oxidoreductase
NAD_binding_8 PF13450 64 - 90 1.8e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 212 - 484 2.1e-117 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1502
AarI CACCTGC 3 cut(s) 101, 1159, 1214
Acc16I TGCGCA 1 cut(s) 677
Acc36I ACCTGC 3 cut(s) 101, 1159, 1214
AccB1I GGYRCC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 764
AciI CCGC 2 cut(s) 951, 1414
AclWI GGATC 5 cut(s) 140, 556, 969, 1122, 1253
AcoI YGGCCR 4 cut(s) 462, 885, 948, 1411
AcsI RAATTY 1 cut(s) 670
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 2 cut(s) 935, 1235
AfiI CCNNNNNNNGG 3 cut(s) 764, 1019, 1067
AgsI TTSAA 8 cut(s) 103, 266, 497, 669, 704, 898, 1048, 1172
AjnI CCWGG 3 cut(s) 313, 847, 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 1275, 1307
AloI GAACNNNNNNTCC 2 cut(s) 619, 651
AluBI AGCT 4 cut(s) 218, 515, 748, 872
AluI AGCT 4 cut(s) 218, 515, 748, 872
Alw21I GWGCWC 2 cut(s) 118, 694
Alw26I GTCTC 2 cut(s) 264, 777
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 5 cut(s) 140, 556, 969, 1122, 1253
AoxI GGCC 4 cut(s) 462, 885, 948, 1411
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 5 cut(s) 107, 215, 310, 660, 998
ApoI RAATTY 1 cut(s) 670
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 3 cut(s) 678, 913, 1025
AspS9I GGNCC 1 cut(s) 1328
AsuC2I CCSGG 2 cut(s) 466, 1112
AsuHPI GGTGA 6 cut(s) 155, 604, 783, 890, 1245, 1428
AvaII GGWCC 1 cut(s) 1328
AxyI CCTNAGG 1 cut(s) 915
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 887
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 118, 694
BbvI GCAGC 5 cut(s) 94, 227, 322, 647, 985
BccI CCATC 5 cut(s) 404, 650, 803, 818, 1406
BceAI ACGGC 1 cut(s) 263
BcgI CGANNNNNNTGC 2 cut(s) 348, 382
BciT130I CCWGG 3 cut(s) 315, 849, 1020
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 2 cut(s) 466, 1112
BcoDI GTCTC 2 cut(s) 264, 777
BfaI CTAG 2 cut(s) 347, 873
BfmI CTRYAG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 914
BfuAI ACCTGC 3 cut(s) 101, 1159, 1214
BfuI GTATCC 1 cut(s) 412
BglI GCCNNNNNGGC 1 cut(s) 1311
BglII AGATCT 1 cut(s) 1559
BisI GCNGC 7 cut(s) 108, 216, 311, 661, 951, 999, 1414
BlsI GCNGC 7 cut(s) 109, 217, 312, 662, 952, 1000, 1415
Bme1390I CCNGG 5 cut(s) 315, 466, 849, 1020, 1112
Bme18I GGWCC 1 cut(s) 1328
BmgT120I GGNCC 1 cut(s) 1328
BmiI GGNNCC 2 cut(s) 199, 921
BmrFI CCNGG 5 cut(s) 315, 466, 849, 1020, 1112
BmsI GCATC 6 cut(s) 364, 1066, 1135, 1259, 1323, 1494
BpmI CTGGAG 1 cut(s) 633
BpuEI CTTGAG 2 cut(s) 295, 1492
BpuMI CCSGG 2 cut(s) 466, 1112
Bsa29I ATCGAT 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 6 cut(s) 237, 314, 438, 822, 1083, 1306
BsaXI ACNNNNNCTCC 2 cut(s) 619, 649
Bsc4I CCNNNNNNNGG 3 cut(s) 764, 1019, 1067
Bse1I ACTGG 1 cut(s) 1331
Bse21I CCTNAGG 1 cut(s) 915
BseBI CCWGG 3 cut(s) 315, 849, 1020
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 6 cut(s) 237, 314, 438, 822, 1083, 1306
BseGI GGATG 2 cut(s) 795, 1057
BseLI CCNNNNNNNGG 3 cut(s) 764, 1019, 1067
BseMII CTCAG 1 cut(s) 929
BseNI ACTGG 1 cut(s) 1331
BseRI GAGGAG 6 cut(s) 179, 182, 484, 632, 1086, 1554
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 5 cut(s) 94, 227, 322, 647, 985
BseYI CCCAGC 2 cut(s) 26, 46
BsgI GTGCAG 1 cut(s) 234
BshFI GGCC 4 cut(s) 464, 887, 950, 1413
BshNI GGYRCC 1 cut(s) 197
BshVI ATCGAT 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 118, 694
BsiSI CCGG 4 cut(s) 155, 201, 465, 1111
BslFI GGGAC 1 cut(s) 693
BslI CCNNNNNNNGG 3 cut(s) 764, 1019, 1067
BsmAI GTCTC 2 cut(s) 264, 777
BsmFI GGGAC 1 cut(s) 693
BsmI GAATGC 1 cut(s) 507
BsnI GGCC 4 cut(s) 464, 887, 950, 1413
Bso31I GGTCTC 1 cut(s) 777
Bsp1286I GDGCHC 3 cut(s) 53, 118, 694
Bsp1407I TGTACA 2 cut(s) 933, 1233
Bsp143I GATC 9 cut(s) 66, 132, 548, 729, 974, 1114, 1258, 1467, 1559
BspACI CCGC 2 cut(s) 951, 1414
BspANI GGCC 4 cut(s) 464, 887, 950, 1413
BspCNI CTCAG 1 cut(s) 928
BspDI ATCGAT 1 cut(s) 180
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 199, 921
BspMAI CTGCAG 1 cut(s) 112
BspMI ACCTGC 3 cut(s) 101, 1159, 1214
BspPI GGATC 5 cut(s) 140, 556, 969, 1122, 1253
BspT107I GGYRCC 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 777
BsrGI TGTACA 2 cut(s) 933, 1233
BsrI ACTGG 1 cut(s) 1331
BssECI CCNNGG 6 cut(s) 237, 314, 438, 822, 1083, 1306
BssMI GATC 9 cut(s) 66, 132, 548, 729, 974, 1114, 1258, 1467, 1559
BssT1I CCWWGG 3 cut(s) 237, 438, 1306
Bst2UI CCWGG 3 cut(s) 315, 849, 1020
Bst4CI ACNGT 6 cut(s) 143, 452, 743, 904, 933, 1084
Bst6I CTCTTC 1 cut(s) 1344
BstAPI GCANNNNNTGC 1 cut(s) 221
BstAUI TGTACA 2 cut(s) 933, 1233
BstDEI CTNAG 3 cut(s) 686, 915, 1121
BstDSI CCRYGG 1 cut(s) 1083
BstENI CCTNNNNNAGG 1 cut(s) 1065
BstF5I GGATG 2 cut(s) 795, 1057
BstH2I RGCGCY 1 cut(s) 914
BstHHI GCGC 3 cut(s) 678, 913, 1025
BstKTI GATC 9 cut(s) 69, 135, 551, 732, 977, 1117, 1261, 1470, 1562
BstMAI GTCTC 2 cut(s) 264, 777
BstMBI GATC 9 cut(s) 66, 132, 548, 729, 974, 1114, 1258, 1467, 1559
BstMWI GCNNNNNNNGC 6 cut(s) 113, 221, 660, 947, 1302, 1311
BstNI CCWGG 3 cut(s) 315, 849, 1020
BstNSI RCATGY 3 cut(s) 260, 773, 1450
BstSCI CCNGG 5 cut(s) 313, 464, 847, 1018, 1110
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 1 cut(s) 118
BstV1I GCAGC 5 cut(s) 94, 227, 322, 647, 985
BstX2I RGATCY 3 cut(s) 974, 1114, 1559
BstYI RGATCY 3 cut(s) 974, 1114, 1559
Bsu15I ATCGAT 1 cut(s) 180
Bsu36I CCTNAGG 1 cut(s) 915
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 4 cut(s) 464, 887, 950, 1413
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 1083
BtgZI GCGATG 1 cut(s) 669
BtsCI GGATG 2 cut(s) 795, 1057
BtsI GCAGTG 1 cut(s) 1184
BtsIMutI CAGTG 2 cut(s) 558, 1184
BveI ACCTGC 3 cut(s) 101, 1159, 1214
CciI TCATGA 1 cut(s) 63
CfoI GCGC 3 cut(s) 678, 913, 1025
Cfr13I GGNCC 1 cut(s) 1328
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 1 cut(s) 1467
Csp6I GTAC 2 cut(s) 934, 1234
CviAII CATG 8 cut(s) 64, 257, 764, 770, 979, 994, 1051, 1447
CviQI GTAC 2 cut(s) 934, 1234
DdeI CTNAG 3 cut(s) 686, 915, 1121
DpnI GATC 9 cut(s) 68, 134, 550, 731, 976, 1116, 1260, 1469, 1561
DpnII GATC 9 cut(s) 66, 132, 548, 729, 974, 1114, 1258, 1467, 1559
EaeI YGGCCR 4 cut(s) 462, 885, 948, 1411
Eam1104I CTCTTC 1 cut(s) 1344
EarI CTCTTC 1 cut(s) 1344
Eco130I CCWWGG 3 cut(s) 237, 438, 1306
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 777
Eco32I GATATC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 1328
Eco57I CTGAAG 1 cut(s) 849
Eco81I CCTNAGG 1 cut(s) 915
EcoNI CCTNNNNNAGG 1 cut(s) 1065
EcoRII CCWGG 3 cut(s) 313, 847, 1018
EcoRV GATATC 1 cut(s) 323
EcoT14I CCWWGG 3 cut(s) 237, 438, 1306
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 3 cut(s) 237, 438, 1306
FaeI CATG 8 cut(s) 67, 260, 767, 773, 982, 997, 1054, 1450
FalI AAGNNNNNCTT 2 cut(s) 697, 729
FaqI GGGAC 1 cut(s) 693
FatI CATG 8 cut(s) 63, 256, 763, 769, 978, 993, 1050, 1446
FauNDI CATATG 1 cut(s) 631
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 7 cut(s) 108, 216, 311, 661, 951, 999, 1414
FokI GGATG 2 cut(s) 782, 1044
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 7 cut(s) 108, 216, 311, 661, 951, 999, 1414
FspBI CTAG 2 cut(s) 347, 873
FspI TGCGCA 1 cut(s) 677
GlaI GCGC 3 cut(s) 677, 912, 1024
GluI GCNGC 7 cut(s) 108, 216, 311, 661, 951, 999, 1414
GsaI CCCAGC 2 cut(s) 30, 50
GsuI CTGGAG 1 cut(s) 633
HaeII RGCGCY 1 cut(s) 914
HaeIII GGCC 4 cut(s) 464, 887, 950, 1413
HapII CCGG 4 cut(s) 155, 201, 465, 1111
HgaI GACGC 1 cut(s) 1467
HhaI GCGC 3 cut(s) 678, 913, 1025
Hin1II CATG 8 cut(s) 67, 260, 767, 773, 982, 997, 1054, 1450
Hin6I GCGC 3 cut(s) 676, 911, 1023
HinP1I GCGC 3 cut(s) 676, 911, 1023
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HinfI GANTC 3 cut(s) 365, 1040, 1172
HpaII CCGG 4 cut(s) 155, 201, 465, 1111
HphI GGTGA 6 cut(s) 155, 604, 783, 890, 1245, 1428
Hpy166II GTNNAC 2 cut(s) 116, 958
Hpy188I TCNGA 4 cut(s) 137, 457, 974, 1096
Hpy188III TCNNGA 4 cut(s) 64, 347, 1471, 1495
Hpy8I GTNNAC 2 cut(s) 116, 958
HpyAV CCTTC 6 cut(s) 236, 409, 798, 873, 1443, 1505
HpyCH4III ACNGT 6 cut(s) 143, 452, 743, 904, 933, 1084
HpyF10VI GCNNNNNNNGC 6 cut(s) 113, 221, 660, 947, 1302, 1311
HpyF3I CTNAG 3 cut(s) 686, 915, 1121
Hsp92II CATG 8 cut(s) 67, 260, 767, 773, 982, 997, 1054, 1450
HspAI GCGC 3 cut(s) 676, 911, 1023
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 9 cut(s) 66, 132, 548, 729, 974, 1114, 1258, 1467, 1559
LmnI GCTCC 2 cut(s) 640, 697
Lsp1109I GCAGC 5 cut(s) 94, 227, 322, 647, 985
LweI GCATC 6 cut(s) 364, 1066, 1135, 1259, 1323, 1494
MaeI CTAG 2 cut(s) 347, 873
MaeIII GTNAC 1 cut(s) 553
MalI GATC 9 cut(s) 68, 134, 550, 731, 976, 1116, 1260, 1469, 1561
MboI GATC 9 cut(s) 66, 132, 548, 729, 974, 1114, 1258, 1467, 1559
MboII GAAGA 5 cut(s) 52, 362, 1097, 1109, 1331
MflI RGATCY 3 cut(s) 974, 1114, 1559
MhlI GDGCHC 3 cut(s) 53, 118, 694
MlsI TGGCCA 1 cut(s) 887
MluCI AATT 8 cut(s) 98, 261, 291, 329, 670, 1026, 1162, 1515
MluNI TGGCCA 1 cut(s) 887
MlyI GAGTC 1 cut(s) 374
Mox20I TGGCCA 1 cut(s) 887
MroXI GAANNNNTTC 1 cut(s) 98
MscI TGGCCA 1 cut(s) 887
MseI TTAA 3 cut(s) 1064, 1353, 1418
MslI CAYNNNNRTG 1 cut(s) 768
Msp20I TGGCCA 1 cut(s) 887
MspA1I CMGCKG 1 cut(s) 953
MspI CCGG 4 cut(s) 155, 201, 465, 1111
MspR9I CCNGG 5 cut(s) 315, 466, 849, 1020, 1112
Mva1269I GAATGC 1 cut(s) 507
MvaI CCWGG 3 cut(s) 315, 849, 1020
MwoI GCNNNNNNNGC 6 cut(s) 113, 221, 660, 947, 1302, 1311
NciI CCSGG 2 cut(s) 466, 1112
NdeI CATATG 1 cut(s) 631
NdeII GATC 9 cut(s) 66, 132, 548, 729, 974, 1114, 1258, 1467, 1559
NlaIII CATG 8 cut(s) 67, 260, 767, 773, 982, 997, 1054, 1450
NlaIV GGNNCC 2 cut(s) 199, 921
NmuCI GTSAC 1 cut(s) 553
NsbI TGCGCA 1 cut(s) 677
NspI RCATGY 3 cut(s) 260, 773, 1450
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 3 cut(s) 101, 1159, 1214
PctI GAATGC 1 cut(s) 507
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 2 cut(s) 1040, 1172
PflMI CCANNNNNTGG 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 1110
PkrI GCNGC 7 cut(s) 109, 217, 312, 662, 952, 1000, 1415
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
PsiI TTATAA 1 cut(s) 1502
Psp6I CCWGG 3 cut(s) 313, 847, 1018
PspFI CCCAGC 2 cut(s) 26, 46
PspGI CCWGG 3 cut(s) 313, 847, 1018
PspN4I GGNNCC 2 cut(s) 199, 921
PspPI GGNCC 1 cut(s) 1328
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 3 cut(s) 974, 1114, 1559
RsaI GTAC 2 cut(s) 935, 1235
RsaNI GTAC 2 cut(s) 934, 1234
RseI CAYNNNNRTG 1 cut(s) 768
SaqAI TTAA 3 cut(s) 1064, 1353, 1418
SatI GCNGC 7 cut(s) 108, 216, 311, 661, 951, 999, 1414
Sau3AI GATC 9 cut(s) 66, 132, 548, 729, 974, 1114, 1258, 1467, 1559
Sau96I GGNCC 1 cut(s) 1328
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 5 cut(s) 315, 466, 849, 1020, 1112
SduI GDGCHC 3 cut(s) 53, 118, 694
SfaNI GCATC 6 cut(s) 364, 1066, 1135, 1259, 1323, 1494
SfcI CTRYAG 1 cut(s) 108
SinI GGWCC 1 cut(s) 1328
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 2 cut(s) 274, 1471
SmoI CTYRAG 2 cut(s) 274, 1471
Sse9I AATT 8 cut(s) 98, 261, 291, 329, 670, 1026, 1162, 1515
SsiI CCGC 2 cut(s) 951, 1414
SspMI CTAG 2 cut(s) 347, 873
StyD4I CCNGG 5 cut(s) 313, 464, 847, 1018, 1110
StyI CCWWGG 3 cut(s) 237, 438, 1306
TaaI ACNGT 6 cut(s) 143, 452, 743, 904, 933, 1084
TaqI TCGA 3 cut(s) 180, 368, 1462
TasI AATT 8 cut(s) 98, 261, 291, 329, 670, 1026, 1162, 1515
TatI WGTACW 2 cut(s) 933, 1233
TauI GCSGC 2 cut(s) 953, 1416
TfiI GAWTC 2 cut(s) 1040, 1172
Tru1I TTAA 3 cut(s) 1064, 1353, 1418
Tru9I TTAA 3 cut(s) 1064, 1353, 1418
TscAI CASTG 2 cut(s) 558, 1191
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 5 cut(s) 107, 215, 310, 660, 998
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 4 cut(s) 52, 481, 1032, 1562
TspGWI ACGGA 1 cut(s) 1416
TspRI CASTG 2 cut(s) 558, 1191
Van91I CCANNNNNTGG 1 cut(s) 764
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 1 cut(s) 1328
XagI CCTNNNNNAGG 1 cut(s) 1065
XapI RAATTY 1 cut(s) 670
XbaI TCTAGA 1 cut(s) 346
XceI RCATGY 3 cut(s) 260, 773, 1450
XcmI CCANNNNNNNNNTGG 3 cut(s) 386, 535, 1216
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 347, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.