Rroxscaffold_7G00214500

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000108
Physical Location & Seq
Reverse (-)
65146356 .. 65149383
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214500.1

Sequence Viewer

Length: 789 bp
ATGCGTGAAAAAGATGCAACTCTTTCAAATGTCAAATTGGAACAAGGATCAGTGACAACACTAATCGAGGAGAAGGGCATTGTCAAAGGGGTGATTTACAAGAACACAGCTGGAGAGGAGATGAGAACATATGCTCCACTAACAATTGTGTGTGATGGCTGCTTTTCAAATCTGCGCAAATCTCTCAGTGATCCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGGTGTTAGAGAACTGTGATCTTCCCCATGCAAAACATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTGTTTTACCCTATTAGTTCCACTGAGATTCATTGTTTGGTCGATGTACCTGGGACAAAAGTACCATCAGTTGCTAGTGGCGAAATGGCTAATTATTTGAGGACTGTGGTGGCTCCTCAGGTTCCCCCACAGCTATATAATGCTTTTATGGTTGCAGTAGAGAAAGGAAACATTAGATCCATGCAAAACAAAAGCATGGATGCTAATCCTGTTCCCACTCCTGGAGCAATTTTGTTGGGGGACTCATTCAACATGAGGCATCCCTTAACAGGAGGAGGAATGACCGTGGCTCTTTCAGACATAGTTCTTCTCCGTGATCTTCTTGGACCCCTATGTGATCTTAATGATGCACCTGCTTTATGCAATTACCTTGAATCATTTTATACATTGCGCAAGCCTGTGTCATCTACTATAAACACATTTACAACATGTTTTGTGCATCGCCTGATCCAGCAAGACAGGAAATGCGTGAAGCATGTTTTGGCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

262

Amino Acids

28.53

Weight (kDa)

6.64

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 17 - 207 1.5e-06 FAD binding domain
SE PF08491 45 - 253 2.4e-85 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 658
Acc16I TGCGCA 2 cut(s) 176, 689
Acc36I ACCTGC 1 cut(s) 658
AclWI GGATC 4 cut(s) 55, 185, 468, 739
AfaI GTAC 2 cut(s) 345, 360
AfiI CCNNNNNNNGG 2 cut(s) 518, 566
AflIII ACRYGT 2 cut(s) 268, 725
AgsI TTSAA 5 cut(s) 27, 168, 203, 547, 671
AjnI CCWGG 2 cut(s) 346, 517
AjuI GAANNNNNNNTTGG 1 cut(s) 761
AloI GAACNNNNNNTCC 2 cut(s) 118, 150
AluBI AGCT 2 cut(s) 110, 430
AluI AGCT 2 cut(s) 110, 430
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 4 cut(s) 55, 185, 468, 739
ApeKI GCWGC 1 cut(s) 159
AspLEI GCGC 2 cut(s) 177, 690
AspS9I GGNCC 1 cut(s) 623
AsuHPI GGTGA 2 cut(s) 103, 282
AvaII GGWCC 1 cut(s) 623
AxyI CCTNAGG 1 cut(s) 414
BaeI ACNNNNGTAYC 2 cut(s) 342, 375
BbvI GCAGC 1 cut(s) 146
BccI CCATC 3 cut(s) 149, 302, 370
BciT130I CCWGG 2 cut(s) 348, 519
BcoDI GTCTC 1 cut(s) 276
BfaI CTAG 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 658
BisI GCNGC 1 cut(s) 160
BlsI GCNGC 1 cut(s) 161
Bme1390I CCNGG 2 cut(s) 348, 519
Bme18I GGWCC 1 cut(s) 623
BmgT120I GGNCC 1 cut(s) 623
BmiI GGNNCC 3 cut(s) 411, 420, 625
BmrFI CCNGG 2 cut(s) 348, 519
BmsI GCATC 5 cut(s) 4, 487, 565, 634, 745
BpmI CTGGAG 2 cut(s) 132, 540
BsaBI GATNNNNATC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 347, 582
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bsc4I CCNNNNNNNGG 2 cut(s) 518, 566
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 1 cut(s) 683
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 2 cut(s) 348, 519
BseDI CCNNGG 2 cut(s) 347, 582
BseGI GGATG 3 cut(s) 294, 502, 556
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 2 cut(s) 518, 566
BseMI GCAATG 1 cut(s) 683
BseMII CTCAG 3 cut(s) 199, 312, 428
BseRI GAGGAG 4 cut(s) 83, 131, 402, 585
BseXI GCAGC 1 cut(s) 146
BslFI GGGAC 3 cut(s) 192, 364, 551
BslI CCNNNNNNNGG 2 cut(s) 518, 566
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 3 cut(s) 192, 364, 551
Bso31I GGTCTC 1 cut(s) 276
Bsp143I GATC 7 cut(s) 47, 190, 244, 473, 613, 634, 744
BspCNI CTCAG 3 cut(s) 198, 313, 427
BspLI GGNNCC 3 cut(s) 411, 420, 625
BspMI ACCTGC 1 cut(s) 658
BspPI GGATC 4 cut(s) 55, 185, 468, 739
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 683
BssECI CCNNGG 2 cut(s) 347, 582
BssMI GATC 7 cut(s) 47, 190, 244, 473, 613, 634, 744
Bst2UI CCWGG 2 cut(s) 348, 519
Bst4CI ACNGT 3 cut(s) 242, 403, 583
BstC8I GCNNGC 1 cut(s) 692
BstDEI CTNAG 3 cut(s) 185, 321, 414
BstDSI CCRYGG 1 cut(s) 582
BstF5I GGATG 3 cut(s) 294, 502, 556
BstHHI GCGC 2 cut(s) 177, 690
BstKTI GATC 7 cut(s) 50, 193, 247, 476, 616, 637, 747
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 7 cut(s) 47, 190, 244, 473, 613, 634, 744
BstNI CCWGG 2 cut(s) 348, 519
BstNSI RCATGY 3 cut(s) 272, 729, 776
BstSCI CCNGG 2 cut(s) 346, 517
BstV1I GCAGC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
Bsu36I CCTNAGG 1 cut(s) 414
BtgI CCRYGG 1 cut(s) 582
BtgZI GCGATG 1 cut(s) 722
BtsCI GGATG 3 cut(s) 294, 502, 556
BtsIMutI CAGTG 3 cut(s) 57, 193, 318
BveI ACCTGC 1 cut(s) 658
Cac8I GCNNGC 1 cut(s) 692
CfoI GCGC 2 cut(s) 177, 690
Cfr13I GGNCC 1 cut(s) 623
Csp6I GTAC 2 cut(s) 344, 359
CviAII CATG 8 cut(s) 254, 263, 269, 478, 493, 550, 726, 773
CviJI RGCY 8 cut(s) 110, 159, 386, 410, 430, 587, 694, 782
CviKI_1 RGCY 8 cut(s) 110, 159, 386, 410, 430, 587, 694, 782
CviQI GTAC 2 cut(s) 344, 359
DdeI CTNAG 3 cut(s) 185, 321, 414
DpnI GATC 7 cut(s) 49, 192, 246, 475, 615, 636, 746
DpnII GATC 7 cut(s) 47, 190, 244, 473, 613, 634, 744
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 623
Eco81I CCTNAGG 1 cut(s) 414
EcoRII CCWGG 2 cut(s) 346, 517
FaeI CATG 8 cut(s) 257, 266, 272, 481, 496, 553, 729, 776
FalI AAGNNNNNCTT 2 cut(s) 196, 228
FaqI GGGAC 3 cut(s) 192, 364, 551
FatI CATG 8 cut(s) 253, 262, 268, 477, 492, 549, 725, 772
FauNDI CATATG 1 cut(s) 130
Fnu4HI GCNGC 1 cut(s) 160
FokI GGATG 3 cut(s) 281, 509, 543
Fsp4HI GCNGC 1 cut(s) 160
FspBI CTAG 1 cut(s) 372
FspI TGCGCA 2 cut(s) 176, 689
GlaI GCGC 2 cut(s) 176, 689
GluI GCNGC 1 cut(s) 160
GsuI CTGGAG 2 cut(s) 132, 540
HhaI GCGC 2 cut(s) 177, 690
Hin1II CATG 8 cut(s) 257, 266, 272, 481, 496, 553, 729, 776
Hin6I GCGC 2 cut(s) 175, 688
HinP1I GCGC 2 cut(s) 175, 688
HinfI GANTC 3 cut(s) 325, 539, 671
HphI GGTGA 2 cut(s) 103, 282
Hpy188I TCNGA 1 cut(s) 595
HpyAV CCTTC 2 cut(s) 67, 297
HpyCH4III ACNGT 3 cut(s) 242, 403, 583
HpyCH4V TGCA 7 cut(s) 17, 257, 452, 481, 647, 660, 736
HpyF3I CTNAG 3 cut(s) 185, 321, 414
Hsp92II CATG 8 cut(s) 257, 266, 272, 481, 496, 553, 729, 776
HspAI GCGC 2 cut(s) 175, 688
Kzo9I GATC 7 cut(s) 47, 190, 244, 473, 613, 634, 744
LmnI GCTCC 3 cut(s) 139, 415, 521
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 5 cut(s) 4, 487, 565, 634, 745
MaeI CTAG 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 52
MalI GATC 7 cut(s) 49, 192, 246, 475, 615, 636, 746
MboI GATC 7 cut(s) 47, 190, 244, 473, 613, 634, 744
MboII GAAGA 3 cut(s) 239, 596, 608
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 1 cut(s) 473
MluCI AATT 5 cut(s) 35, 144, 388, 525, 661
MlyI GAGTC 1 cut(s) 533
MnlI CCTC 8 cut(s) 61, 109, 220, 390, 423, 546, 563, 566
MseI TTAA 2 cut(s) 563, 639
MslI CAYNNNNRTG 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 110
MspR9I CCNGG 2 cut(s) 348, 519
MunI CAATTG 1 cut(s) 144
MvaI CCWGG 2 cut(s) 348, 519
NdeI CATATG 1 cut(s) 130
NdeII GATC 7 cut(s) 47, 190, 244, 473, 613, 634, 744
NlaIII CATG 8 cut(s) 257, 266, 272, 481, 496, 553, 729, 776
NlaIV GGNNCC 3 cut(s) 411, 420, 625
NmuCI GTSAC 1 cut(s) 52
NsbI TGCGCA 2 cut(s) 176, 689
NspI RCATGY 3 cut(s) 272, 729, 776
PaqCI CACCTGC 1 cut(s) 658
PciI ACATGT 2 cut(s) 268, 725
PfeI GAWTC 2 cut(s) 325, 671
PfoI TCCNGGA 1 cut(s) 517
PkrI GCNGC 1 cut(s) 161
PleI GAGTC 1 cut(s) 533
PpsI GAGTC 1 cut(s) 533
PscI ACATGT 2 cut(s) 268, 725
Psp6I CCWGG 2 cut(s) 346, 517
PspGI CCWGG 2 cut(s) 346, 517
PspN4I GGNNCC 3 cut(s) 411, 420, 625
PspPI GGNCC 1 cut(s) 623
PsuI RGATCY 1 cut(s) 473
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 2 cut(s) 345, 360
RsaNI GTAC 2 cut(s) 344, 359
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 2 cut(s) 563, 639
SatI GCNGC 1 cut(s) 160
Sau3AI GATC 7 cut(s) 47, 190, 244, 473, 613, 634, 744
Sau96I GGNCC 1 cut(s) 623
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 2 cut(s) 348, 519
SetI ASST 7 cut(s) 112, 201, 349, 420, 432, 652, 669
SfaNI GCATC 5 cut(s) 4, 487, 565, 634, 745
SinI GGWCC 1 cut(s) 623
SmiMI CAYNNNNRTG 1 cut(s) 267
Sse9I AATT 5 cut(s) 35, 144, 388, 525, 661
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 2 cut(s) 346, 517
TaaI ACNGT 3 cut(s) 242, 403, 583
TaqI TCGA 2 cut(s) 66, 339
TasI AATT 5 cut(s) 35, 144, 388, 525, 661
TfiI GAWTC 2 cut(s) 325, 671
Tru1I TTAA 2 cut(s) 563, 639
Tru9I TTAA 2 cut(s) 563, 639
TscAI CASTG 3 cut(s) 57, 193, 325
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 159
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 1 cut(s) 317
TspGWI ACGGA 1 cut(s) 599
TspRI CASTG 3 cut(s) 57, 193, 325
VpaK11BI GGWCC 1 cut(s) 623
XceI RCATGY 3 cut(s) 272, 729, 776
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.