Rh6BG031000

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000108
Physical Location & Seq
Forward (+)
4691748 .. 4692768
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG031000.1

Sequence Viewer

Length: 501 bp
ATGACCGTGGCTCTTTCAGACATTGTTCTTCTCCGGGATCTTCTTAGACCCCTGCATAATATCAATGATGCACCTGCTTTGTGCAATTACCTTGAATCATTTTACACACTGCGTAAGCCTGTGTCATCTACCATAAACACATTGGCAGGTGCATTGTACAAGGTGTTTTGTGCATCACCTGATCCAGCAAGACAGGAAATGCGTGAAGCCTGTTTTAGCTATTTGAGCCTTGGAGGCATCTGCTCATATGGACCAGTATCTCTTCTCTCTGGTCTTAACCCTCGTCCATTACACTTGTTTCTCCATTTCTTTGCAGTTGCCATCTATGGTGTTGGCCGCTTAATGCTTCCGGTCCCTTCACCTAAACGCATGTGTCTTGGTTTTGGATTGATCTTGAGTGCATCAGGCATCATATTCCCAATTATAAAGGGTGAAGGAATTAGACAGATGTTCTTTCCTGCCACAATACCAGCATATTACAGATCTCCTCCTGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

166

Amino Acids

18.25

Weight (kDa)

9.25

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SE PF08491 1 - 120 1.8e-44 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AarI CACCTGC 2 cut(s) 82, 137
Acc36I ACCTGC 2 cut(s) 82, 137
AciI CCGC 1 cut(s) 337
AclWI GGATC 2 cut(s) 45, 176
AcoI YGGCCR 1 cut(s) 334
AfaI GTAC 1 cut(s) 158
AgsI TTSAA 1 cut(s) 95
AluBI AGCT 1 cut(s) 219
AluI AGCT 1 cut(s) 219
AlwI GGATC 2 cut(s) 45, 176
AoxI GGCC 1 cut(s) 334
AspS9I GGNCC 2 cut(s) 251, 352
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 3 cut(s) 168, 351, 443
AvaII GGWCC 2 cut(s) 251, 352
BccI CCATC 1 cut(s) 329
BcnI CCSGG 1 cut(s) 35
BfuAI ACCTGC 2 cut(s) 82, 137
BglI GCCNNNNNGGC 1 cut(s) 234
BglII AGATCT 1 cut(s) 482
BisI GCNGC 1 cut(s) 337
BlsI GCNGC 1 cut(s) 338
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 2 cut(s) 251, 352
BmgT120I GGNCC 2 cut(s) 251, 352
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 1 cut(s) 35
BmsI GCATC 5 cut(s) 58, 182, 246, 410, 417
BpuEI CTTGAG 1 cut(s) 415
BpuMI CCSGG 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 6, 229
BsaWI WCCGGW 1 cut(s) 349
Bse1I ACTGG 1 cut(s) 254
BseDI CCNNGG 2 cut(s) 6, 229
BseNI ACTGG 1 cut(s) 254
BseRI GAGGAG 1 cut(s) 477
BshFI GGCC 1 cut(s) 336
BsiSI CCGG 2 cut(s) 34, 350
BslFI GGGAC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 1 cut(s) 336
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 4 cut(s) 37, 181, 390, 482
BspACI CCGC 1 cut(s) 337
BspANI GGCC 1 cut(s) 336
BspLI GGNNCC 1 cut(s) 354
BspMI ACCTGC 2 cut(s) 82, 137
BspPI GGATC 2 cut(s) 45, 176
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 6, 229
BssMI GATC 4 cut(s) 37, 181, 390, 482
BssT1I CCWWGG 1 cut(s) 229
Bst4CI ACNGT 1 cut(s) 7
Bst6I CTCTTC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 44
BstDSI CCRYGG 1 cut(s) 6
BstKTI GATC 4 cut(s) 40, 184, 393, 485
BstMBI GATC 4 cut(s) 37, 181, 390, 482
BstMWI GCNNNNNNNGC 2 cut(s) 225, 234
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 1 cut(s) 33
BstX2I RGATCY 2 cut(s) 37, 482
BstYI RGATCY 2 cut(s) 37, 482
BsuRI GGCC 1 cut(s) 336
BtgI CCRYGG 1 cut(s) 6
BtsI GCAGTG 1 cut(s) 107
BtsIMutI CAGTG 1 cut(s) 107
BveI ACCTGC 2 cut(s) 82, 137
Cfr13I GGNCC 2 cut(s) 251, 352
Csp6I GTAC 1 cut(s) 157
CviAII CATG 1 cut(s) 370
CviJI RGCY 6 cut(s) 11, 118, 209, 219, 228, 336
CviKI_1 RGCY 6 cut(s) 11, 118, 209, 219, 228, 336
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 1 cut(s) 44
DpnI GATC 4 cut(s) 39, 183, 392, 484
DpnII GATC 4 cut(s) 37, 181, 390, 482
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 251, 352
EcoT14I CCWWGG 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 1 cut(s) 373
FaiI YATR 9 cut(s) 57, 134, 247, 249, 327, 371, 413, 425, 475
FaqI GGGAC 1 cut(s) 338
FatI CATG 1 cut(s) 369
FauNDI CATATG 1 cut(s) 247
Fnu4HI GCNGC 1 cut(s) 337
Fsp4HI GCNGC 1 cut(s) 337
GluI GCNGC 1 cut(s) 337
HaeIII GGCC 1 cut(s) 336
HapII CCGG 2 cut(s) 34, 350
Hin1II CATG 1 cut(s) 373
HinfI GANTC 1 cut(s) 95
HpaII CCGG 2 cut(s) 34, 350
HphI GGTGA 3 cut(s) 168, 351, 443
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 1 cut(s) 394
HpyAV CCTTC 2 cut(s) 366, 428
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4V TGCA 7 cut(s) 55, 71, 84, 152, 173, 314, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 234
HpyF3I CTNAG 1 cut(s) 44
Hsp92II CATG 1 cut(s) 373
Kzo9I GATC 4 cut(s) 37, 181, 390, 482
LweI GCATC 5 cut(s) 58, 182, 246, 410, 417
MalI GATC 4 cut(s) 39, 183, 392, 484
MboI GATC 4 cut(s) 37, 181, 390, 482
MboII GAAGA 3 cut(s) 20, 32, 254
MflI RGATCY 2 cut(s) 37, 482
MluCI AATT 3 cut(s) 85, 420, 438
MnlI CCTC 3 cut(s) 227, 291, 498
MseI TTAA 2 cut(s) 276, 341
MspI CCGG 2 cut(s) 34, 350
MspR9I CCNGG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 225, 234
NciI CCSGG 1 cut(s) 35
NdeI CATATG 1 cut(s) 247
NdeII GATC 4 cut(s) 37, 181, 390, 482
NlaIII CATG 1 cut(s) 373
NlaIV GGNNCC 1 cut(s) 354
NspI RCATGY 1 cut(s) 373
PaqCI CACCTGC 2 cut(s) 82, 137
PfeI GAWTC 1 cut(s) 95
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 1 cut(s) 338
PsiI TTATAA 1 cut(s) 425
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 2 cut(s) 251, 352
PsuI RGATCY 2 cut(s) 37, 482
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
SaqAI TTAA 2 cut(s) 276, 341
SatI GCNGC 1 cut(s) 337
Sau3AI GATC 4 cut(s) 37, 181, 390, 482
Sau96I GGNCC 2 cut(s) 251, 352
ScrFI CCNGG 1 cut(s) 35
SetI ASST 7 cut(s) 76, 93, 151, 165, 181, 221, 364
SfaNI GCATC 5 cut(s) 58, 182, 246, 410, 417
SinI GGWCC 2 cut(s) 251, 352
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
Sse9I AATT 3 cut(s) 85, 420, 438
SsiI CCGC 1 cut(s) 337
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 7
TasI AATT 3 cut(s) 85, 420, 438
TatI WGTACW 1 cut(s) 156
TauI GCSGC 1 cut(s) 339
TfiI GAWTC 1 cut(s) 95
Tru1I TTAA 2 cut(s) 276, 341
Tru9I TTAA 2 cut(s) 276, 341
TscAI CASTG 1 cut(s) 114
TspDTI ATGAA 1 cut(s) 485
TspRI CASTG 1 cut(s) 114
VpaK11BI GGWCC 2 cut(s) 251, 352
XceI RCATGY 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.