Rw4G014000

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000108
Physical Location & Seq
Forward (+)
32715993 .. 32719133
3141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014000.1

Sequence Viewer

Length: 1722 bp
ATGCTTCCAAACACAGCTCTATATAAACCCACTCAACCTCCCCAACTGTCCATAAGTCCTTCACTTCCCAAACAAAACTCAAAAACTAAATCAGAGAACAGAACTAAATTCCCGTTCTTCATTCCTATAATCCTATTCCTAATTAGTTTTGTTGAAGATGGTGTCTTACGAGTATATTCTGGTGGGTTCTTAGCTTCTTTGCTTGCCTTTGTTTTCTTCTTGATCATCAGTACTACTCTTGGTGGAAACAAAAAGGTCAAAGCTTTAAGTGGAGGAGCAAAGAGTAATGGGTTTCAGCCGGAAATGGAGGAGGACAAAAGTACCGACGTCGTCATTGTCGGCGCCGGAGTTGCCGGTGCAGCTCTAGCTTACACTCTTGCCAAGGAAGGACGCCGTGTGCATGTCATCGAAAGAGACTTAACTGAGCCAGACAGAATTGTTGGTGAGCTGTTGCAGCCTGGGGGTTATCTCAAGTTGATTGAGTTGGGTCTTGAGGACTGTGCAAATGAGTCCATTGATGCTCAGAAGGTGTTTGGTTATGCTCTTTACAAAGATGGGAAGGATACAAAACTGTCTTACCCCTTGGAAAAGTACAGTGCAGATGTCGCCGGGAGAAGTTTCCACAATGGTCGTTTCATTCAAAGAATGCGAGAAAAAGCTGCAACTCTTGCAAATGTAAAATTGGAACAAGGATCAGTGACAACACTAATTGAGGAGAAGGGCATTGTCAAAGGCGTGATTTACAAGAACAAGGCTGGAGAGGAGCTGAGAGCATATGCTCCACTAACAATCGTGTGTGATGGATGCTTTTCAAATCTGCGCAAATCTCTCAGTGCTCCAAAGGTTGAAAATCCCTCTTGTTTTGTTGGTTTGATTTTGGAGAACTGTGACCTTCCACATGCAAATCATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTGTTTTATCCCATCAGTAACACTGAGGTTCGTTGTTTGGTAGATGTACCTGGAACAAAAGTGCCTTCAGTAGCTAATGGTGAAATGGCCAAGTATTTGAAATCTGCGGTGGCTCCTCAGATTCCCCCACAGCTGCACAATGCTTTTATGGCAGCAGTAGAGAAAGGAAACATCAGAACAATGCAAAACAAAAGCATGGCTGCTAATCCTGTTCCCACTCCTGGTGCAATTTTATTGGGGGATGCATTCAACATGAGGCATCCATTAACAGGAGGAGGAATGACTGTGGCTCTTTCAGACATTGTTCTTCTCCGTAATCTTCTTAGACCCCTACGTGATCTCAGTGACGCAACTGCTTTGTGCAATTACCTTGAAGCATTCTACACACTTCGTAAGCCTGTGTCATCTACCATAAACACATTGGCAGGTGCTTTATACAAGGTGTTTTGTGCATCACCTGATCCAGCAAAACAGGAAATGCGTGAAGCATGTTTTGACTATCTGAGCCTTGGAGGTATCTGTTCATATGGACCAGTATCTCTGCTCTCTGGTCTTAACCCTCGTCCCCTGCACTTATTTCTCCATTTCTTTGCCGTTGCCATCTATGGCGTCGGACGCTTAATGCTCCCCTTCCCTTCACCTCAGCGCATGTGGCTTGGGGCTAGATTGATCTTGAGTGCATCAAGCATCATATTCCCGATTATTAAGGGGGAAGGAGTTAGACAGATGTTCTTTCCTGCAACAGTGCCAGCATTTTACAGATCTCCTCCTCTTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

573

Amino Acids

62.32

Weight (kDa)

9.07

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 108 - 426 7.9e-21 FAD binding domain
DAO PF01266 109 - 139 6.9e-08 FAD dependent oxidoreductase
NAD_binding_8 PF13450 112 - 138 2.6e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 260 - 532 3.2e-119 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1358
AatII GACGTC 1 cut(s) 330
Acc16I TGCGCA 1 cut(s) 821
Acc36I ACCTGC 1 cut(s) 1358
AccB1I GGYRCC 1 cut(s) 341
AciI CCGC 1 cut(s) 1049
AclWI GGATC 2 cut(s) 700, 1397
AcoI YGGCCR 1 cut(s) 1029
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 1 cut(s) 993
AcyI GRCGYC 4 cut(s) 327, 342, 391, 1551
AfaI GTAC 4 cut(s) 232, 322, 593, 990
AfiI CCNNNNNNNGG 2 cut(s) 1163, 1211
AflIII ACRYGT 1 cut(s) 913
AgsI TTSAA 7 cut(s) 155, 641, 813, 848, 1042, 1192, 1316
AjnI CCWGG 3 cut(s) 457, 991, 1162
Alw21I GWGCWC 1 cut(s) 838
Alw26I GTCTC 2 cut(s) 408, 921
AlwI GGATC 2 cut(s) 700, 1397
AoxI GGCC 1 cut(s) 1029
ApeKI GCWGC 6 cut(s) 359, 454, 659, 1075, 1094, 1142
ApoI RAATTY 1 cut(s) 107
AspLEI GCGC 3 cut(s) 344, 822, 1590
AspS9I GGNCC 1 cut(s) 1472
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 5 cut(s) 455, 927, 1034, 1389, 1572
AvaII GGWCC 1 cut(s) 1472
BaeI ACNNNNGTAYC 2 cut(s) 304, 337
BalI TGGCCA 1 cut(s) 1031
BanI GGYRCC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 838
BbvCI CCTCAGC 1 cut(s) 1584
BbvI GCAGC 6 cut(s) 371, 466, 646, 1062, 1106, 1129
BccI CCATC 6 cut(s) 152, 548, 794, 947, 962, 1550
BceAI ACGGC 2 cut(s) 378, 1520
BciT130I CCWGG 3 cut(s) 459, 993, 1164
BciVI GTATCC 1 cut(s) 556
BclI TGATCA 1 cut(s) 222
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 2 cut(s) 408, 921
BfaI CTAG 2 cut(s) 365, 1605
BfoI RGCGCY 1 cut(s) 345
BfuAI ACCTGC 1 cut(s) 1358
BfuI GTATCC 1 cut(s) 556
BglII AGATCT 1 cut(s) 1703
BisI GCNGC 6 cut(s) 360, 455, 660, 1076, 1095, 1143
BlsI GCNGC 6 cut(s) 361, 456, 661, 1077, 1096, 1144
BmcAI AGTACT 1 cut(s) 232
Bme1390I CCNGG 4 cut(s) 459, 610, 993, 1164
Bme18I GGWCC 1 cut(s) 1472
BmgT120I GGNCC 1 cut(s) 1472
BmiI GGNNCC 2 cut(s) 343, 1056
BmrFI CCNGG 4 cut(s) 459, 610, 993, 1164
BmsI GCATC 7 cut(s) 508, 794, 1174, 1210, 1403, 1631, 1638
BpmI CTGGAG 1 cut(s) 777
Bpu10I CCTNAGC 1 cut(s) 1584
BpuEI CTTGAG 3 cut(s) 455, 512, 1636
BpuMI CCSGG 1 cut(s) 610
BsaAI YACGTR 1 cut(s) 1277
BsaHI GRCGYC 4 cut(s) 327, 342, 391, 1551
BsaI GGTCTC 1 cut(s) 921
BsaJI CCNNGG 4 cut(s) 381, 458, 582, 1450
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 2 cut(s) 1163, 1211
Bse118I RCCGGY 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 1475
BseBI CCWGG 3 cut(s) 459, 993, 1164
BseDI CCNNGG 4 cut(s) 381, 458, 582, 1450
BseGI GGATG 4 cut(s) 809, 939, 1189, 1201
BseLI CCNNNNNNNGG 2 cut(s) 1163, 1211
BseMII CTCAG 9 cut(s) 414, 536, 758, 844, 957, 1073, 1297, 1436, 1598
BseNI ACTGG 1 cut(s) 1475
BseRI GAGGAG 8 cut(s) 288, 323, 728, 776, 1047, 1230, 1698, 1701
BseXI GCAGC 6 cut(s) 371, 466, 646, 1062, 1106, 1129
BsgI GTGCAG 4 cut(s) 378, 618, 1061, 1496
BshFI GGCC 1 cut(s) 1031
BshNI GGYRCC 1 cut(s) 341
BsiHKAI GWGCWC 1 cut(s) 838
BsiSI CCGG 4 cut(s) 299, 345, 354, 609
BslFI GGGAC 1 cut(s) 1491
BslI CCNNNNNNNGG 2 cut(s) 1163, 1211
BsmAI GTCTC 2 cut(s) 408, 921
BsmFI GGGAC 1 cut(s) 1491
BsmI GAATGC 3 cut(s) 651, 1187, 1319
BsnI GGCC 1 cut(s) 1031
Bso31I GGTCTC 1 cut(s) 921
Bsp1286I GDGCHC 1 cut(s) 838
Bsp143I GATC 6 cut(s) 222, 692, 1279, 1402, 1611, 1703
BspACI CCGC 1 cut(s) 1049
BspANI GGCC 1 cut(s) 1031
BspCNI CTCAG 9 cut(s) 415, 535, 759, 843, 958, 1072, 1296, 1437, 1597
BspLI GGNNCC 2 cut(s) 343, 1056
BspMI ACCTGC 1 cut(s) 1358
BspPI GGATC 2 cut(s) 700, 1397
BspT107I GGYRCC 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 921
BsrFI RCCGGY 1 cut(s) 353
BsrI ACTGG 1 cut(s) 1475
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 4 cut(s) 381, 458, 582, 1450
BssMI GATC 6 cut(s) 222, 692, 1279, 1402, 1611, 1703
BssNI GRCGYC 4 cut(s) 327, 342, 391, 1551
BssT1I CCWWGG 3 cut(s) 381, 582, 1450
Bst2UI CCWGG 3 cut(s) 459, 993, 1164
Bst4CI ACNGT 7 cut(s) 48, 500, 573, 596, 887, 1228, 1687
Bst6I CTCTTC 1 cut(s) 1719
BstACI GRCGYC 4 cut(s) 327, 342, 391, 1551
BstAPI GCANNNNNTGC 1 cut(s) 668
BstBAI YACGTR 1 cut(s) 1277
BstC8I GCNNGC 2 cut(s) 204, 1692
BstF5I GGATG 4 cut(s) 809, 939, 1189, 1201
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 3 cut(s) 344, 822, 1590
BstKTI GATC 6 cut(s) 225, 695, 1282, 1405, 1614, 1706
BstMAI GTCTC 2 cut(s) 408, 921
BstMBI GATC 6 cut(s) 222, 692, 1279, 1402, 1611, 1703
BstMWI GCNNNNNNNGC 9 cut(s) 350, 359, 365, 454, 605, 668, 1091, 1557, 1594
BstNI CCWGG 3 cut(s) 459, 993, 1164
BstNSI RCATGY 5 cut(s) 404, 902, 917, 1434, 1594
BstSCI CCNGG 4 cut(s) 457, 608, 991, 1162
BstV1I GCAGC 6 cut(s) 371, 466, 646, 1062, 1106, 1129
BstX2I RGATCY 1 cut(s) 1703
BstYI RGATCY 1 cut(s) 1703
BsuI GTATCC 1 cut(s) 556
BsuRI GGCC 1 cut(s) 1031
BtsCI GGATG 4 cut(s) 809, 939, 1189, 1201
BtsIMutI CAGTG 7 cut(s) 601, 702, 838, 963, 1291, 1692, 1717
BveI ACCTGC 1 cut(s) 1358
Cac8I GCNNGC 2 cut(s) 204, 1692
CfoI GCGC 3 cut(s) 344, 822, 1590
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 1472
CseI GACGC 4 cut(s) 399, 1298, 1540, 1566
Csp6I GTAC 4 cut(s) 231, 321, 592, 989
CviAII CATG 8 cut(s) 401, 899, 908, 914, 1138, 1195, 1431, 1591
CviQI GTAC 4 cut(s) 231, 321, 592, 989
DinI GGCGCC 1 cut(s) 343
DpnI GATC 6 cut(s) 224, 694, 1281, 1404, 1613, 1705
DpnII GATC 6 cut(s) 222, 692, 1279, 1402, 1611, 1703
EaeI YGGCCR 1 cut(s) 1029
Eam1104I CTCTTC 1 cut(s) 1719
EarI CTCTTC 1 cut(s) 1719
Eco130I CCWWGG 3 cut(s) 381, 582, 1450
Eco31I GGTCTC 1 cut(s) 921
Eco47I GGWCC 1 cut(s) 1472
Eco57I CTGAAG 1 cut(s) 993
EcoRII CCWGG 3 cut(s) 457, 991, 1162
EcoT14I CCWWGG 3 cut(s) 381, 582, 1450
EcoT22I ATGCAT 1 cut(s) 1189
EgeI GGCGCC 1 cut(s) 343
EheI GGCGCC 1 cut(s) 343
ErhI CCWWGG 3 cut(s) 381, 582, 1450
FaeI CATG 8 cut(s) 404, 902, 911, 917, 1141, 1198, 1434, 1594
FaqI GGGAC 1 cut(s) 1491
FatI CATG 8 cut(s) 400, 898, 907, 913, 1137, 1194, 1430, 1590
FauNDI CATATG 2 cut(s) 775, 1468
FbaI TGATCA 1 cut(s) 222
Fnu4HI GCNGC 6 cut(s) 360, 455, 660, 1076, 1095, 1143
FokI GGATG 4 cut(s) 816, 926, 1188, 1196
Fsp4HI GCNGC 6 cut(s) 360, 455, 660, 1076, 1095, 1143
FspBI CTAG 2 cut(s) 365, 1605
FspI TGCGCA 1 cut(s) 821
GlaI GCGC 3 cut(s) 343, 821, 1589
GluI GCNGC 6 cut(s) 360, 455, 660, 1076, 1095, 1143
GsuI CTGGAG 1 cut(s) 777
HaeII RGCGCY 1 cut(s) 345
HaeIII GGCC 1 cut(s) 1031
HapII CCGG 4 cut(s) 299, 345, 354, 609
HgaI GACGC 4 cut(s) 399, 1298, 1540, 1566
HhaI GCGC 3 cut(s) 344, 822, 1590
Hin1I GRCGYC 4 cut(s) 327, 342, 391, 1551
Hin1II CATG 8 cut(s) 404, 902, 911, 917, 1141, 1198, 1434, 1594
Hin6I GCGC 3 cut(s) 342, 820, 1588
HinP1I GCGC 3 cut(s) 342, 820, 1588
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 2 cut(s) 509, 1063
HpaII CCGG 4 cut(s) 299, 345, 354, 609
HphI GGTGA 5 cut(s) 455, 927, 1034, 1389, 1572
Hpy188I TCNGA 7 cut(s) 94, 525, 1062, 1118, 1240, 1446, 1556
Hpy188III TCNNGA 4 cut(s) 220, 491, 1615, 1639
Hpy99I CGWCG 3 cut(s) 329, 332, 1556
HpyCH4III ACNGT 7 cut(s) 48, 500, 573, 596, 887, 1228, 1687
HpyCH4IV ACGT 2 cut(s) 327, 1276
HpyF10VI GCNNNNNNNGC 9 cut(s) 350, 359, 365, 454, 605, 668, 1091, 1557, 1594
HpySE526I ACGT 2 cut(s) 327, 1276
Hsp92I GRCGYC 4 cut(s) 327, 342, 391, 1551
Hsp92II CATG 8 cut(s) 404, 902, 911, 917, 1141, 1198, 1434, 1594
HspAI GCGC 3 cut(s) 342, 820, 1588
KasI GGCGCC 1 cut(s) 341
Ksp22I TGATCA 1 cut(s) 222
Kzo9I GATC 6 cut(s) 222, 692, 1279, 1402, 1611, 1703
LmnI GCTCC 6 cut(s) 275, 763, 784, 841, 1060, 1572
Lsp1109I GCAGC 6 cut(s) 371, 466, 646, 1062, 1106, 1129
LweI GCATC 7 cut(s) 508, 794, 1174, 1210, 1403, 1631, 1638
MaeI CTAG 2 cut(s) 365, 1605
MaeII ACGT 2 cut(s) 327, 1276
MaeIII GTNAC 4 cut(s) 697, 887, 959, 1286
MalI GATC 6 cut(s) 224, 694, 1281, 1404, 1613, 1705
MboI GATC 6 cut(s) 222, 692, 1279, 1402, 1611, 1703
MboII GAAGA 6 cut(s) 109, 167, 208, 1241, 1253, 1706
MflI RGATCY 1 cut(s) 1703
MhlI GDGCHC 1 cut(s) 838
MlsI TGGCCA 1 cut(s) 1031
MluCI AATT 7 cut(s) 107, 141, 435, 680, 708, 1170, 1306
MluNI TGGCCA 1 cut(s) 1031
Mly113I GGCGCC 1 cut(s) 342
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 1 cut(s) 1534
Mox20I TGGCCA 1 cut(s) 1031
Mph1103I ATGCAT 1 cut(s) 1189
MscI TGGCCA 1 cut(s) 1031
MseI TTAA 6 cut(s) 266, 419, 1208, 1497, 1562, 1647
MslI CAYNNNNRTG 1 cut(s) 912
Msp20I TGGCCA 1 cut(s) 1031
MspA1I CMGCKG 1 cut(s) 1075
MspI CCGG 4 cut(s) 299, 345, 354, 609
MspR9I CCNGG 4 cut(s) 459, 610, 993, 1164
Mva1269I GAATGC 3 cut(s) 651, 1187, 1319
MvaI CCWGG 3 cut(s) 459, 993, 1164
MwoI GCNNNNNNNGC 9 cut(s) 350, 359, 365, 454, 605, 668, 1091, 1557, 1594
NarI GGCGCC 1 cut(s) 342
NciI CCSGG 1 cut(s) 610
NdeI CATATG 2 cut(s) 775, 1468
NdeII GATC 6 cut(s) 222, 692, 1279, 1402, 1611, 1703
NlaIII CATG 8 cut(s) 404, 902, 911, 917, 1141, 1198, 1434, 1594
NlaIV GGNNCC 2 cut(s) 343, 1056
NmuCI GTSAC 3 cut(s) 697, 887, 1286
NsbI TGCGCA 1 cut(s) 821
NsiI ATGCAT 1 cut(s) 1189
NspI RCATGY 5 cut(s) 404, 902, 917, 1434, 1594
PaqCI CACCTGC 1 cut(s) 1358
PciI ACATGT 1 cut(s) 913
PctI GAATGC 3 cut(s) 651, 1187, 1319
PfeI GAWTC 1 cut(s) 1063
PflFI GACNNNGTC 1 cut(s) 329
PkrI GCNGC 6 cut(s) 361, 456, 661, 1077, 1096, 1144
PleI GAGTC 1 cut(s) 517
PluTI GGCGCC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 517
Ppu21I YACGTR 1 cut(s) 1277
PscI ACATGT 1 cut(s) 913
Psp6I CCWGG 3 cut(s) 457, 991, 1162
PspGI CCWGG 3 cut(s) 457, 991, 1162
PspN4I GGNNCC 2 cut(s) 343, 1056
PspPI GGNCC 1 cut(s) 1472
PsuI RGATCY 1 cut(s) 1703
PsyI GACNNNGTC 1 cut(s) 329
PvuII CAGCTG 1 cut(s) 1075
RsaI GTAC 4 cut(s) 232, 322, 593, 990
RsaNI GTAC 4 cut(s) 231, 321, 592, 989
RseI CAYNNNNRTG 1 cut(s) 912
SaqAI TTAA 6 cut(s) 266, 419, 1208, 1497, 1562, 1647
SatI GCNGC 6 cut(s) 360, 455, 660, 1076, 1095, 1143
Sau3AI GATC 6 cut(s) 222, 692, 1279, 1402, 1611, 1703
Sau96I GGNCC 1 cut(s) 1472
ScaI AGTACT 1 cut(s) 232
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 4 cut(s) 459, 610, 993, 1164
SduI GDGCHC 1 cut(s) 838
SfaNI GCATC 7 cut(s) 508, 794, 1174, 1210, 1403, 1631, 1638
SfoI GGCGCC 1 cut(s) 343
SinI GGWCC 1 cut(s) 1472
SmiMI CAYNNNNRTG 1 cut(s) 912
SmlI CTYRAG 3 cut(s) 470, 491, 1615
SmoI CTYRAG 3 cut(s) 470, 491, 1615
Sse9I AATT 7 cut(s) 107, 141, 435, 680, 708, 1170, 1306
SsiI CCGC 1 cut(s) 1049
SspDI GGCGCC 1 cut(s) 341
SspMI CTAG 2 cut(s) 365, 1605
StyD4I CCNGG 4 cut(s) 457, 608, 991, 1162
StyI CCWWGG 3 cut(s) 381, 582, 1450
TaaI ACNGT 7 cut(s) 48, 500, 573, 596, 887, 1228, 1687
TaiI ACGT 2 cut(s) 330, 1279
TaqI TCGA 1 cut(s) 408
TasI AATT 7 cut(s) 107, 141, 435, 680, 708, 1170, 1306
TatI WGTACW 2 cut(s) 230, 591
TfiI GAWTC 1 cut(s) 1063
Tru1I TTAA 6 cut(s) 266, 419, 1208, 1497, 1562, 1647
Tru9I TTAA 6 cut(s) 266, 419, 1208, 1497, 1562, 1647
TscAI CASTG 6 cut(s) 601, 702, 838, 970, 1291, 1692
TseFI GTSAC 3 cut(s) 697, 887, 1286
TseI GCWGC 6 cut(s) 359, 454, 659, 1075, 1094, 1142
Tsp45I GTSAC 3 cut(s) 697, 887, 1286
TspDTI ATGAA 3 cut(s) 109, 625, 1455
TspGWI ACGGA 1 cut(s) 1244
TspRI CASTG 6 cut(s) 601, 702, 838, 970, 1291, 1692
Tth111I GACNNNGTC 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 1472
XapI RAATTY 1 cut(s) 107
XceI RCATGY 5 cut(s) 404, 902, 917, 1434, 1594
XcmI CCANNNNNNNNNTGG 1 cut(s) 1360
XspI CTAG 2 cut(s) 365, 1605
ZraI GACGTC 1 cut(s) 328
ZrmI AGTACT 1 cut(s) 232
Zsp2I ATGCAT 1 cut(s) 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.