FvH4_4g14050

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000108
Physical Location & Seq
Forward (+)
17584474 .. 17587802
3329 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14050.t1

Sequence Viewer

Length: 1299 bp
ATGGTTTCTTATGAGTATGTTTTTGGCGGGGTCTTGGTTTCATTGCTGGGATCTGTTTTCTTCATGATCATCAGCACTACTTTGGGAGGAAAGAAACCATGCAAGGCTCCAAGTAGAGGAGTTGAGAGTAATGATGGGTTTGGAAACGGTGCGTTTCAGCCGGAAATGGAGGAGGAGAAAAGTACCGATGTTGTTATTGTCGGCGCTGGAGTTGCCGGTGCAGCTCTTGCCTACACTCTTGCCAAGGAAGGTCGTCGTGTACATGTAATCGAAAGAGACTTGACCGAGCCAGACAGAATTGTTGGTGAGCTGTTGCAGCCTGGGGGTTATCTCAAGTTGATTGAGTTGGGTCTCGAGGATTGTGCAAATGAGTCAATTGATGCGCAGAAGGTGTTTGGTTATGCTCTTTACAAAGATGGCAAGGATACAAAACTGACTTATCCCTTGGAACAATATAGTTCAGATGTGGCTGGGAGAAGTTTCCACAATGGGCGTTTCATCCAAAGAATGCGCGAAAAAGCAACGACTCTATCAAATAGCACCGAGGTTCGTTGTTTGGTGGATGTACCTGGAACAAAAGTGCCTTCAGTAGCTAGTGGTGAAATGGCTAAATACCTGAAAACTGTTGTGGCCCCTCAGATTCCCCCACAGCTGCACAATGCTTTTATGGCCGCAGTAGAGAAGGGAAACATTAGGACAATGCAAAACAGAAGCATGGCTGCTAATCCTGTCCCTACTCCTGGTGCAATTTTATTAGGTGATGCGTTCAACATGAGGCATCCTTTAACAGGAGGAGGAATGACTGGTGCTCTTTCAGACATCGTTCTTCTCCGTGATCTTCTTAGACCCCTACGTGATCTCAATGATGCACCTGCTTTATGCAATTACCTTGAAGCATTCTACACACTGCGCAAGCCTGTGTCATCTACCATAAACACACTGGCAGGTGCTTTGTACAAGGTGTTTTGTGCATCACCCGATCCAGCAAGACAAGAAATGCGGGAAGCTTGTTTTGGCTATTTGAGCTTTGGAGGCATCTGTTCATATGGATCAGTATCTCTTCTCTCTGGTCTTAACCCTCGTCCGCTGCACTTGTTTCTCCATTTCTTTGCTGTTGCCATCTATGGTGTCGGCCGCTTAATGCTTCCGTTCCCTTCACCTAAACGAATGTGGCTTGGAATTCGATTGATCTTGAGTGCATCAGGCATCATATTGCCCATTATAAAGGGTGAAGGGGTCAGACAGATGTTCTTTCCTGCCACAGTACCAGCATATTACAGATCTCCTCCCGTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

433

Amino Acids

46.93

Weight (kDa)

8.81

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 62 - 150 4.3e-07 FAD binding domain
DAO PF01266 63 - 93 4.9e-08 FAD dependent oxidoreductase
FAD_binding_2 PF00890 63 - 92 8.8e-06 FAD binding domain
NAD_binding_8 PF13450 66 - 92 1.6e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 180 - 391 1.1e-83 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1223
AarI CACCTGC 2 cut(s) 880, 935
Acc16I TGCGCA 2 cut(s) 384, 911
Acc36I ACCTGC 2 cut(s) 880, 935
AccII CGCG 1 cut(s) 513
AciI CCGC 5 cut(s) 27, 672, 1000, 1085, 1135
AclWI GGATC 3 cut(s) 58, 974, 1057
AcoI YGGCCR 2 cut(s) 669, 1132
AcsI RAATTY 1 cut(s) 1179
AcuI CTGAAG 1 cut(s) 570
AfaI GTAC 5 cut(s) 184, 261, 567, 956, 1266
AfiI CCNNNNNNNGG 3 cut(s) 116, 740, 788
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 2 cut(s) 769, 893
AjnI CCWGG 3 cut(s) 319, 568, 739
AjuI GAANNNNNNNTTGG 2 cut(s) 996, 1028
AluBI AGCT 6 cut(s) 224, 310, 593, 652, 1007, 1026
AluI AGCT 6 cut(s) 224, 310, 593, 652, 1007, 1026
Alw21I GWGCWC 1 cut(s) 811
Alw26I GTCTC 2 cut(s) 270, 356
AlwI GGATC 3 cut(s) 58, 974, 1057
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 3 cut(s) 630, 669, 1132
ApeKI GCWGC 5 cut(s) 221, 316, 652, 719, 1087
ApoI RAATTY 1 cut(s) 1179
AspLEI GCGC 4 cut(s) 206, 385, 513, 912
AspS9I GGNCC 1 cut(s) 631
AsuHPI GGTGA 6 cut(s) 317, 611, 770, 966, 1149, 1241
AvaI CYCGRG 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 811
BbvI GCAGC 5 cut(s) 233, 328, 639, 706, 1074
BccI CCATC 3 cut(s) 128, 410, 1127
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 3 cut(s) 321, 570, 741
BciVI GTATCC 1 cut(s) 418
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 270, 356
BfaI CTAG 1 cut(s) 594
BfoI RGCGCY 1 cut(s) 207
BfuAI ACCTGC 2 cut(s) 880, 935
BfuI GTATCC 1 cut(s) 418
BglII AGATCT 1 cut(s) 1280
BisI GCNGC 7 cut(s) 222, 317, 653, 672, 720, 1088, 1135
BlsI GCNGC 7 cut(s) 223, 318, 654, 673, 721, 1089, 1136
Bme1390I CCNGG 3 cut(s) 321, 570, 741
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 631
BmiI GGNNCC 2 cut(s) 108, 633
BmrFI CCNGG 3 cut(s) 321, 570, 741
BmsI GCATC 8 cut(s) 370, 751, 787, 856, 980, 1044, 1208, 1215
BpmI CTGGAG 1 cut(s) 228
BpuEI CTTGAG 2 cut(s) 317, 1213
BsaAI YACGTR 1 cut(s) 854
BsaBI GATNNNNATC 1 cut(s) 1054
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 4 cut(s) 243, 320, 444, 543
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 740, 788
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 808, 945
Bse3DI GCAATG 1 cut(s) 41
Bse8I GATNNNNATC 1 cut(s) 1054
BseBI CCWGG 3 cut(s) 321, 570, 741
BseDI CCNNGG 4 cut(s) 243, 320, 444, 543
BseGI GGATG 3 cut(s) 498, 568, 778
BseJI GATNNNNATC 1 cut(s) 1054
BseLI CCNNNNNNNGG 3 cut(s) 116, 740, 788
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 2 cut(s) 808, 945
BseRI GAGGAG 5 cut(s) 132, 185, 188, 807, 1275
BseX3I CGGCCG 1 cut(s) 1132
BseXI GCAGC 5 cut(s) 233, 328, 639, 706, 1074
BseYI CCCAGC 2 cut(s) 46, 470
BsgI GTGCAG 3 cut(s) 240, 638, 1073
Bsh1236I CGCG 1 cut(s) 513
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 3 cut(s) 632, 671, 1134
BsiEI CGRYCG 1 cut(s) 1135
BsiHKAI GWGCWC 1 cut(s) 811
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 2 cut(s) 161, 216
BslFI GGGAC 1 cut(s) 716
BslI CCNNNNNNNGG 3 cut(s) 116, 740, 788
BsmAI GTCTC 2 cut(s) 270, 356
BsmFI GGGAC 1 cut(s) 716
BsmI GAATGC 2 cut(s) 513, 896
BsnI GGCC 3 cut(s) 632, 671, 1134
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 811
Bsp1407I TGTACA 2 cut(s) 259, 954
Bsp143I GATC 8 cut(s) 50, 66, 835, 856, 979, 1049, 1188, 1280
BspACI CCGC 5 cut(s) 27, 672, 1000, 1085, 1135
BspANI GGCC 3 cut(s) 632, 671, 1134
BspCNI CTCAG 1 cut(s) 649
BspFNI CGCG 1 cut(s) 513
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 2 cut(s) 108, 633
BspMI ACCTGC 2 cut(s) 880, 935
BspPI GGATC 3 cut(s) 58, 974, 1057
BspTNI GGTCTC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 215
BsrGI TGTACA 2 cut(s) 259, 954
BsrI ACTGG 2 cut(s) 808, 945
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 4 cut(s) 243, 320, 444, 543
BssMI GATC 8 cut(s) 50, 66, 835, 856, 979, 1049, 1188, 1280
BssT1I CCWWGG 2 cut(s) 243, 444
Bst2UI CCWGG 3 cut(s) 321, 570, 741
Bst4CI ACNGT 3 cut(s) 149, 625, 1264
Bst6I CTCTTC 1 cut(s) 1065
BstAPI GCANNNNNTGC 1 cut(s) 227
BstAUI TGTACA 2 cut(s) 259, 954
BstBAI YACGTR 1 cut(s) 854
BstC8I GCNNGC 1 cut(s) 914
BstDEI CTNAG 2 cut(s) 636, 842
BstENI CCTNNNNNAGG 1 cut(s) 786
BstF5I GGATG 3 cut(s) 498, 568, 778
BstFNI CGCG 1 cut(s) 513
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 4 cut(s) 206, 385, 513, 912
BstKTI GATC 8 cut(s) 53, 69, 838, 859, 982, 1052, 1191, 1283
BstMAI GTCTC 2 cut(s) 270, 356
BstMBI GATC 8 cut(s) 50, 66, 835, 856, 979, 1049, 1188, 1280
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 7 cut(s) 212, 221, 227, 316, 668, 1023, 1032
BstNI CCWGG 3 cut(s) 321, 570, 741
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 3 cut(s) 319, 568, 739
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 5 cut(s) 233, 328, 639, 706, 1074
BstX2I RGATCY 2 cut(s) 50, 1280
BstYI RGATCY 2 cut(s) 50, 1280
BstZI CGGCCG 1 cut(s) 1132
BsuI GTATCC 1 cut(s) 418
BsuRI GGCC 3 cut(s) 632, 671, 1134
BtsCI GGATG 3 cut(s) 498, 568, 778
BtsI GCAGTG 1 cut(s) 905
BtsIMutI CAGTG 2 cut(s) 905, 938
BveI ACCTGC 2 cut(s) 880, 935
Cac8I GCNNGC 1 cut(s) 914
CciI TCATGA 1 cut(s) 63
CfoI GCGC 4 cut(s) 206, 385, 513, 912
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 631
Csp6I GTAC 5 cut(s) 183, 260, 566, 955, 1265
CviAII CATG 5 cut(s) 64, 99, 263, 715, 772
CviQI GTAC 5 cut(s) 183, 260, 566, 955, 1265
DdeI CTNAG 2 cut(s) 636, 842
DpnI GATC 8 cut(s) 52, 68, 837, 858, 981, 1051, 1190, 1282
DpnII GATC 8 cut(s) 50, 66, 835, 856, 979, 1049, 1188, 1280
EaeI YGGCCR 2 cut(s) 669, 1132
EagI CGGCCG 1 cut(s) 1132
Eam1104I CTCTTC 1 cut(s) 1065
EarI CTCTTC 1 cut(s) 1065
EclXI CGGCCG 1 cut(s) 1132
Eco130I CCWWGG 2 cut(s) 243, 444
Eco31I GGTCTC 1 cut(s) 356
Eco52I CGGCCG 1 cut(s) 1132
Eco57I CTGAAG 1 cut(s) 570
Eco88I CYCGRG 1 cut(s) 353
EcoNI CCTNNNNNAGG 1 cut(s) 786
EcoRI GAATTC 1 cut(s) 1179
EcoRII CCWGG 3 cut(s) 319, 568, 739
EcoT14I CCWWGG 2 cut(s) 243, 444
ErhI CCWWGG 2 cut(s) 243, 444
FaeI CATG 5 cut(s) 67, 102, 266, 718, 775
FaqI GGGAC 1 cut(s) 716
FatI CATG 5 cut(s) 63, 98, 262, 714, 771
FauI CCCGC 2 cut(s) 20, 993
FauNDI CATATG 1 cut(s) 1045
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 7 cut(s) 222, 317, 653, 672, 720, 1088, 1135
FokI GGATG 3 cut(s) 485, 575, 765
Fsp4HI GCNGC 7 cut(s) 222, 317, 653, 672, 720, 1088, 1135
FspBI CTAG 1 cut(s) 594
FspI TGCGCA 2 cut(s) 384, 911
GlaI GCGC 4 cut(s) 205, 384, 512, 911
GluI GCNGC 7 cut(s) 222, 317, 653, 672, 720, 1088, 1135
GsaI CCCAGC 2 cut(s) 50, 474
GsuI CTGGAG 1 cut(s) 228
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 3 cut(s) 632, 671, 1134
HapII CCGG 2 cut(s) 161, 216
HhaI GCGC 4 cut(s) 206, 385, 513, 912
Hin1II CATG 5 cut(s) 67, 102, 266, 718, 775
Hin6I GCGC 4 cut(s) 204, 383, 511, 910
HinP1I GCGC 4 cut(s) 204, 383, 511, 910
HindIII AAGCTT 1 cut(s) 1005
HinfI GANTC 3 cut(s) 371, 526, 640
HpaII CCGG 2 cut(s) 161, 216
HphI GGTGA 6 cut(s) 317, 611, 770, 966, 1149, 1241
Hpy166II GTNNAC 1 cut(s) 260
Hpy188I TCNGA 4 cut(s) 463, 639, 817, 1241
Hpy188III TCNNGA 3 cut(s) 64, 353, 1192
Hpy8I GTNNAC 1 cut(s) 260
Hpy99I CGWCG 1 cut(s) 258
HpyAV CCTTC 6 cut(s) 242, 382, 594, 676, 1164, 1226
HpyCH4III ACNGT 3 cut(s) 149, 625, 1264
HpyCH4IV ACGT 1 cut(s) 853
HpyF10VI GCNNNNNNNGC 7 cut(s) 212, 221, 227, 316, 668, 1023, 1032
HpyF3I CTNAG 2 cut(s) 636, 842
HpySE526I ACGT 1 cut(s) 853
Hsp92II CATG 5 cut(s) 67, 102, 266, 718, 775
HspAI GCGC 4 cut(s) 204, 383, 511, 910
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 8 cut(s) 50, 66, 835, 856, 979, 1049, 1188, 1280
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 5 cut(s) 233, 328, 639, 706, 1074
LweI GCATC 8 cut(s) 370, 751, 787, 856, 980, 1044, 1208, 1215
MaeI CTAG 1 cut(s) 594
MaeII ACGT 1 cut(s) 853
MalI GATC 8 cut(s) 52, 68, 837, 858, 981, 1051, 1190, 1282
MboI GATC 8 cut(s) 50, 66, 835, 856, 979, 1049, 1188, 1280
MboII GAAGA 4 cut(s) 52, 818, 830, 1052
MfeI CAATTG 1 cut(s) 375
MflI RGATCY 2 cut(s) 50, 1280
MhlI GDGCHC 1 cut(s) 811
MluCI AATT 5 cut(s) 297, 375, 747, 883, 1179
MlyI GAGTC 2 cut(s) 380, 520
MseI TTAA 3 cut(s) 785, 1074, 1139
MspA1I CMGCKG 2 cut(s) 652, 1087
MspI CCGG 2 cut(s) 161, 216
MspR9I CCNGG 3 cut(s) 321, 570, 741
MunI CAATTG 1 cut(s) 375
Mva1269I GAATGC 2 cut(s) 513, 896
MvaI CCWGG 3 cut(s) 321, 570, 741
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 7 cut(s) 212, 221, 227, 316, 668, 1023, 1032
NdeI CATATG 1 cut(s) 1045
NdeII GATC 8 cut(s) 50, 66, 835, 856, 979, 1049, 1188, 1280
NlaIII CATG 5 cut(s) 67, 102, 266, 718, 775
NlaIV GGNNCC 2 cut(s) 108, 633
NsbI TGCGCA 2 cut(s) 384, 911
NspI RCATGY 1 cut(s) 266
PaeR7I CTCGAG 1 cut(s) 353
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 2 cut(s) 880, 935
PciI ACATGT 1 cut(s) 262
PctI GAATGC 2 cut(s) 513, 896
PfeI GAWTC 1 cut(s) 640
PkrI GCNGC 7 cut(s) 223, 318, 654, 673, 721, 1089, 1136
PleI GAGTC 2 cut(s) 379, 520
PpsI GAGTC 2 cut(s) 379, 520
Ppu21I YACGTR 1 cut(s) 854
PscI ACATGT 1 cut(s) 262
PsiI TTATAA 1 cut(s) 1223
Psp6I CCWGG 3 cut(s) 319, 568, 739
PspFI CCCAGC 2 cut(s) 46, 470
PspGI CCWGG 3 cut(s) 319, 568, 739
PspN4I GGNNCC 2 cut(s) 108, 633
PspPI GGNCC 1 cut(s) 631
PsuI RGATCY 2 cut(s) 50, 1280
PvuII CAGCTG 1 cut(s) 652
RsaI GTAC 5 cut(s) 184, 261, 567, 956, 1266
RsaNI GTAC 5 cut(s) 183, 260, 566, 955, 1265
SaqAI TTAA 3 cut(s) 785, 1074, 1139
SatI GCNGC 7 cut(s) 222, 317, 653, 672, 720, 1088, 1135
Sau3AI GATC 8 cut(s) 50, 66, 835, 856, 979, 1049, 1188, 1280
Sau96I GGNCC 1 cut(s) 631
SchI GAGTC 2 cut(s) 380, 520
ScrFI CCNGG 3 cut(s) 321, 570, 741
SduI GDGCHC 1 cut(s) 811
SfaNI GCATC 8 cut(s) 370, 751, 787, 856, 980, 1044, 1208, 1215
Sfr274I CTCGAG 1 cut(s) 353
SlaI CTCGAG 1 cut(s) 353
SmlI CTYRAG 3 cut(s) 332, 353, 1192
SmoI CTYRAG 3 cut(s) 332, 353, 1192
Sse9I AATT 5 cut(s) 297, 375, 747, 883, 1179
SsiI CCGC 5 cut(s) 27, 672, 1000, 1085, 1135
SspMI CTAG 1 cut(s) 594
StyD4I CCNGG 3 cut(s) 319, 568, 739
StyI CCWWGG 2 cut(s) 243, 444
TaaI ACNGT 3 cut(s) 149, 625, 1264
TaiI ACGT 1 cut(s) 856
TaqI TCGA 3 cut(s) 270, 354, 1183
TaqII GACCGA 1 cut(s) 299
TasI AATT 5 cut(s) 297, 375, 747, 883, 1179
TatI WGTACW 2 cut(s) 259, 954
TauI GCSGC 2 cut(s) 674, 1137
TfiI GAWTC 1 cut(s) 640
Tru1I TTAA 3 cut(s) 785, 1074, 1139
Tru9I TTAA 3 cut(s) 785, 1074, 1139
TscAI CASTG 2 cut(s) 912, 945
TseI GCWGC 5 cut(s) 221, 316, 652, 719, 1087
TspDTI ATGAA 4 cut(s) 30, 52, 487, 1032
TspGWI ACGGA 2 cut(s) 821, 1137
TspRI CASTG 2 cut(s) 912, 945
XagI CCTNNNNNAGG 1 cut(s) 786
XapI RAATTY 1 cut(s) 1179
XceI RCATGY 1 cut(s) 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XhoI CTCGAG 1 cut(s) 353
XspI CTAG 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.