MD05G1112100.v1.1

Squalene monooxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000108
Physical Location & Seq
Reverse (-)
22970009 .. 22973213
3205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1112100.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGATTACCAGTATGTTCTTGGTGGGGTGTTGGTTTCTCTGCTGGGGTCTGTTTTCTTCATGATCATCAACACCACTCTCAGCGGCGAGAAGAAGAAGGTGGTCGAGAAAGATTCCCGCGGCATTAACGGAGATGGGTACGTGAGAATGCCGTCCCAGAACGGCAGTTTTCACCCGGAGATTGCCAGCGGCACGGATGTTGTCATTGTCGGTGCCGGAGTTGCCGGTGCTGCCCTTGCTTACACTCTTGGAAAGGAAGGACGCCGCGTACATGTGATCGAAAGAGACTTGAGTGAGCCAGACAGAATCGTTGGTGAGCTGTTGCAGCCTGGAGGCTATCTCAAATTGATTGAGTTGGGTCTTGAGGACTGTGCAAATGAGTCCGTTGATGCTCAGAAAGTGTTTGGTTATGCTCTTTACAAAAATGGAAATGACACAAAACTGTCTTATCCCTTGGAAAACTATACTTTAGATATCGCCGGAAGAAGTTTCCATAACGGGCGTTTCATCCAAAAAATGCGTGAAAGGGTTGCAACTCTTAAAAATGTGACACTAGAACAAGGAACTGTGACAACCCTAATTGAAGAAAAGGGCACCGTCAAGGGTGTGTTGTACAAGAACAGGGCTGGAAAGGAGATGAGATCGTATGCTCCACTGACAATAGTATGCGATGGGTGCTTTTCAAATCTCCGCCGCAATCTCTGTACTCCAAAGGTTGACAATCCTTCTTGCTTCGTTGGTTTGATCTTGGAGAATTGTGAACTTCCACATGCAAACCATGGACATGTGATTTTGGGAGACCCTTCACCCATCTTATTTTACCCTATCAGTAGTACCGAGGTTCGTTGTTTGGTAGATGTTGCCGGCACAAAAGTACCTTCAGTAGCTAACGGTGAAATGGCCAAGTACTTGAAAACTGTGGTGGCTCCTCAGGTTCCCCCCCAACTCTTGAAGTCTTTTCTAGCGGCGGTCGAGAAGGGAAATATCAGAACAATGCAAAATAAAAGCATGCCTGCTACTCCTGTTCCCACTCCTGGTGCAATTTTATTGGGTGATGCATTCAACATGAGACACCCTTTAACCGGAGGAGGAATGACTGTGGCTCTTTCAGACATTGTTCTTCTAAGGGATCTTCTTAGACCCCTAACTGATCTCAGTGATGCACCAGCTTTGTGTGAATATCTGGAATCATTCTACACGTTGCGCAAGCCCGTTTCATCTACTATAAACACATTGGCCGGTGCCTTGTACAAGGTATTTTGTTCCTCACCTGACCCGGCAAGACAGGAAATGCGAGAAGCATGTTTCGACTATTTGAGCCTTGGAGGCATATGTTCATACGGACCTATATCGCTTCTCTCTGGGCTTAACCCTCGTCCAATCCATCTGTTTCTCCATTTCTTTGCTGTGGCTGTCTATGGAGTCGGCCGCTTGATGATTCCATTCCCTACGCCTAAACGTCTATGGCTCGGGACTAGATTGATCTTGGGTGCATCAGGAATCATATTCCCCATTATCAAGGGTGAAGGAGTTAGACAAATGTTCTTTCCAGCAACAATCCCGGCATATTACAATGCTCCTCCTCTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

531

Amino Acids

57.68

Weight (kDa)

8.61

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 65 - 376 6.1e-17 FAD binding domain
DAO PF01266 66 - 96 1.1e-06 FAD dependent oxidoreductase
SE PF08491 217 - 489 6e-120 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1205
AccB1I GGYRCC 3 cut(s) 212, 593, 1241
AccII CGCG 2 cut(s) 120, 267
AclWI GGATC 1 cut(s) 1137
AcoI YGGCCR 3 cut(s) 900, 1236, 1426
AcuI CTGAAG 1 cut(s) 864
AcyI GRCGYC 1 cut(s) 262
AfaI GTAC 8 cut(s) 140, 270, 614, 706, 835, 876, 908, 1250
AfiI CCNNNNNNNGG 2 cut(s) 1034, 1082
AflIII ACRYGT 3 cut(s) 271, 784, 1197
AgsI TTSAA 6 cut(s) 584, 684, 913, 952, 1063, 1589
AjnI CCWGG 2 cut(s) 328, 1033
AluBI AGCT 3 cut(s) 319, 887, 1169
AluI AGCT 3 cut(s) 319, 887, 1169
Alw26I GTCTC 3 cut(s) 279, 792, 1063
AlwI GGATC 1 cut(s) 1137
Ama87I CYCGRG 1 cut(s) 1469
AoxI GGCC 3 cut(s) 900, 1236, 1426
ApeKI GCWGC 2 cut(s) 230, 325
AspLEI GCGC 1 cut(s) 1206
AspS9I GGNCC 1 cut(s) 1343
AsuC2I CCSGG 3 cut(s) 176, 1277, 1562
AsuHPI GGTGA 7 cut(s) 164, 326, 798, 905, 1064, 1260, 1535
AvaI CYCGRG 1 cut(s) 1469
AvaII GGWCC 1 cut(s) 1343
AxyI CCTNAGG 1 cut(s) 930
BaeGI GKGCMC 1 cut(s) 596
BaeI ACNNNNGTAYC 2 cut(s) 858, 891
BalI TGGCCA 1 cut(s) 902
BanI GGYRCC 3 cut(s) 212, 593, 1241
BbvI GCAGC 2 cut(s) 217, 337
BccI CCATC 4 cut(s) 128, 665, 818, 1392
BceAI ACGGC 2 cut(s) 136, 178
BciT130I CCWGG 2 cut(s) 330, 1035
BclI TGATCA 1 cut(s) 63
BcnI CCSGG 3 cut(s) 176, 1277, 1562
BcoDI GTCTC 3 cut(s) 279, 792, 1063
BfaI CTAG 3 cut(s) 554, 962, 1476
BglI GCCNNNNNGGC 1 cut(s) 1326
BisI GCNGC 9 cut(s) 85, 121, 190, 231, 265, 326, 694, 966, 1429
BlsI GCNGC 9 cut(s) 86, 122, 191, 232, 266, 327, 695, 967, 1430
BmcAI AGTACT 1 cut(s) 908
Bme1390I CCNGG 5 cut(s) 176, 330, 1035, 1277, 1562
Bme18I GGWCC 1 cut(s) 1343
BmeT110I CYCGRG 1 cut(s) 1469
BmgT120I GGNCC 1 cut(s) 1343
BmiI GGNNCC 5 cut(s) 214, 595, 927, 936, 1243
BmrFI CCNGG 5 cut(s) 176, 330, 1035, 1277, 1562
BmsI GCATC 4 cut(s) 379, 1045, 1150, 1502
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BpmI CTGGAG 1 cut(s) 351
BpuEI CTTGAG 2 cut(s) 310, 383
BpuMI CCSGG 3 cut(s) 176, 1277, 1562
BsaAI YACGTR 1 cut(s) 142
BsaHI GRCGYC 1 cut(s) 262
BsaI GGTCTC 1 cut(s) 792
BsaJI CCNNGG 5 cut(s) 118, 453, 778, 837, 1321
BsaWI WCCGGW 1 cut(s) 1082
Bsc4I CCNNNNNNNGG 2 cut(s) 1034, 1082
Bse118I RCCGGY 3 cut(s) 224, 863, 1238
Bse1I ACTGG 1 cut(s) 10
Bse21I CCTNAGG 1 cut(s) 930
BseBI CCWGG 2 cut(s) 330, 1035
BseDI CCNNGG 5 cut(s) 118, 453, 778, 837, 1321
BseGI GGATG 2 cut(s) 202, 507
BseLI CCNNNNNNNGG 2 cut(s) 1034, 1082
BseMII CTCAG 4 cut(s) 94, 407, 944, 1168
BseNI ACTGG 1 cut(s) 10
BseRI GAGGAG 4 cut(s) 918, 1101, 1569, 1572
BseSI GKGCMC 1 cut(s) 596
BseX3I CGGCCG 1 cut(s) 1426
BseXI GCAGC 2 cut(s) 217, 337
BseYI CCCAGC 1 cut(s) 43
Bsh1236I CGCG 2 cut(s) 120, 267
Bsh1285I CGRYCG 2 cut(s) 972, 1429
BshFI GGCC 3 cut(s) 902, 1238, 1428
BshNI GGYRCC 3 cut(s) 212, 593, 1241
BsiEI CGRYCG 2 cut(s) 972, 1429
BsiHKCI CYCGRG 1 cut(s) 1469
BsiSI CCGG 9 cut(s) 176, 216, 225, 480, 864, 1083, 1239, 1277, 1562
BslFI GGGAC 2 cut(s) 139, 1486
BslI CCNNNNNNNGG 2 cut(s) 1034, 1082
BsmAI GTCTC 3 cut(s) 279, 792, 1063
BsmFI GGGAC 2 cut(s) 139, 1486
BsmI GAATGC 2 cut(s) 153, 1058
BsnI GGCC 3 cut(s) 902, 1238, 1428
Bso31I GGTCTC 1 cut(s) 792
BsoBI CYCGRG 1 cut(s) 1469
Bsp1286I GDGCHC 1 cut(s) 596
Bsp1407I TGTACA 2 cut(s) 612, 1248
Bsp143I GATC 7 cut(s) 63, 276, 641, 744, 1129, 1150, 1482
Bsp19I CCATGG 1 cut(s) 778
BspANI GGCC 3 cut(s) 902, 1238, 1428
BspCNI CTCAG 4 cut(s) 93, 406, 943, 1167
BspFNI CGCG 2 cut(s) 120, 267
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 5 cut(s) 214, 595, 927, 936, 1243
BspPI GGATC 1 cut(s) 1137
BspT107I GGYRCC 3 cut(s) 212, 593, 1241
BspTNI GGTCTC 1 cut(s) 792
BsrFI RCCGGY 3 cut(s) 224, 863, 1238
BsrGI TGTACA 2 cut(s) 612, 1248
BsrI ACTGG 1 cut(s) 10
BssAI RCCGGY 3 cut(s) 224, 863, 1238
BssECI CCNNGG 5 cut(s) 118, 453, 778, 837, 1321
BssMI GATC 7 cut(s) 63, 276, 641, 744, 1129, 1150, 1482
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 3 cut(s) 453, 778, 1321
Bst2UI CCWGG 2 cut(s) 330, 1035
Bst4CI ACNGT 7 cut(s) 371, 444, 568, 598, 893, 919, 1099
Bst6I CTCTTC 1 cut(s) 1590
BstACI GRCGYC 1 cut(s) 262
BstAUI TGTACA 2 cut(s) 612, 1248
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 5 cut(s) 187, 865, 1010, 1014, 1208
BstDEI CTNAG 6 cut(s) 80, 393, 930, 1124, 1136, 1154
BstDSI CCRYGG 2 cut(s) 118, 778
BstF5I GGATG 2 cut(s) 202, 507
BstFNI CGCG 2 cut(s) 120, 267
BstHHI GCGC 1 cut(s) 1206
BstKTI GATC 7 cut(s) 66, 279, 644, 747, 1132, 1153, 1485
BstMAI GTCTC 3 cut(s) 279, 792, 1063
BstMBI GATC 7 cut(s) 63, 276, 641, 744, 1129, 1150, 1482
BstMCI CGRYCG 2 cut(s) 972, 1429
BstMWI GCNNNNNNNGC 6 cut(s) 221, 230, 236, 325, 675, 1326
BstNI CCWGG 2 cut(s) 330, 1035
BstNSI RCATGY 5 cut(s) 275, 773, 788, 1012, 1305
BstSCI CCNGG 5 cut(s) 174, 328, 1033, 1275, 1560
BstSLI GKGCMC 1 cut(s) 596
BstUI CGCG 2 cut(s) 120, 267
BstV1I GCAGC 2 cut(s) 217, 337
BstX2I RGATCY 1 cut(s) 1129
BstYI RGATCY 1 cut(s) 1129
BstZI CGGCCG 1 cut(s) 1426
Bsu36I CCTNAGG 1 cut(s) 930
BsuRI GGCC 3 cut(s) 902, 1238, 1428
BtgI CCRYGG 2 cut(s) 118, 778
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 2 cut(s) 202, 507
BtsIMutI CAGTG 2 cut(s) 653, 1162
Cac8I GCNNGC 5 cut(s) 187, 865, 1010, 1014, 1208
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 1206
Cfr10I RCCGGY 3 cut(s) 224, 863, 1238
Cfr13I GGNCC 1 cut(s) 1343
Cfr42I CCGCGG 1 cut(s) 121
CseI GACGC 1 cut(s) 270
Csp6I GTAC 8 cut(s) 139, 269, 613, 705, 834, 875, 907, 1249
CviAII CATG 8 cut(s) 61, 272, 770, 779, 785, 1009, 1066, 1302
CviQI GTAC 8 cut(s) 139, 269, 613, 705, 834, 875, 907, 1249
DdeI CTNAG 6 cut(s) 80, 393, 930, 1124, 1136, 1154
DpnI GATC 7 cut(s) 65, 278, 643, 746, 1131, 1152, 1484
DpnII GATC 7 cut(s) 63, 276, 641, 744, 1129, 1150, 1482
EaeI YGGCCR 3 cut(s) 900, 1236, 1426
EagI CGGCCG 1 cut(s) 1426
Eam1104I CTCTTC 1 cut(s) 1590
EarI CTCTTC 1 cut(s) 1590
EciI GGCGGA 1 cut(s) 680
EclXI CGGCCG 1 cut(s) 1426
Eco130I CCWWGG 3 cut(s) 453, 778, 1321
Eco31I GGTCTC 1 cut(s) 792
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 1343
Eco52I CGGCCG 1 cut(s) 1426
Eco57I CTGAAG 1 cut(s) 864
Eco81I CCTNAGG 1 cut(s) 930
Eco88I CYCGRG 1 cut(s) 1469
EcoRII CCWGG 2 cut(s) 328, 1033
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 3 cut(s) 453, 778, 1321
EcoT22I ATGCAT 1 cut(s) 1060
ErhI CCWWGG 3 cut(s) 453, 778, 1321
FaeI CATG 8 cut(s) 64, 275, 773, 782, 788, 1012, 1069, 1305
FaqI GGGAC 2 cut(s) 139, 1486
FatI CATG 8 cut(s) 60, 271, 769, 778, 784, 1008, 1065, 1301
FauI CCCGC 1 cut(s) 125
FauNDI CATATG 1 cut(s) 1331
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 9 cut(s) 85, 121, 190, 231, 265, 326, 694, 966, 1429
FokI GGATG 2 cut(s) 209, 494
Fsp4HI GCNGC 9 cut(s) 85, 121, 190, 231, 265, 326, 694, 966, 1429
FspBI CTAG 3 cut(s) 554, 962, 1476
FspI TGCGCA 1 cut(s) 1205
GlaI GCGC 1 cut(s) 1205
GluI GCNGC 9 cut(s) 85, 121, 190, 231, 265, 326, 694, 966, 1429
GsaI CCCAGC 1 cut(s) 47
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 3 cut(s) 902, 1238, 1428
HapII CCGG 9 cut(s) 176, 216, 225, 480, 864, 1083, 1239, 1277, 1562
HgaI GACGC 1 cut(s) 270
HhaI GCGC 1 cut(s) 1206
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 8 cut(s) 64, 275, 773, 782, 788, 1012, 1069, 1305
Hin6I GCGC 1 cut(s) 1204
HinP1I GCGC 1 cut(s) 1204
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HinfI GANTC 7 cut(s) 113, 306, 380, 1187, 1422, 1438, 1500
HpaII CCGG 9 cut(s) 176, 216, 225, 480, 864, 1083, 1239, 1277, 1562
HphI GGTGA 7 cut(s) 164, 326, 798, 905, 1064, 1260, 1535
Hpy166II GTNNAC 2 cut(s) 718, 761
Hpy188I TCNGA 3 cut(s) 396, 989, 1111
Hpy188III TCNNGA 8 cut(s) 61, 106, 362, 949, 973, 1184, 1471, 1497
Hpy8I GTNNAC 2 cut(s) 718, 761
HpyAV CCTTC 7 cut(s) 91, 251, 735, 813, 888, 970, 1520
HpyCH4III ACNGT 7 cut(s) 371, 444, 568, 598, 893, 919, 1099
HpyCH4IV ACGT 3 cut(s) 141, 1199, 1459
HpyCH4V TGCA 9 cut(s) 325, 374, 533, 773, 997, 1040, 1058, 1163, 1493
HpyF10VI GCNNNNNNNGC 6 cut(s) 221, 230, 236, 325, 675, 1326
HpyF3I CTNAG 6 cut(s) 80, 393, 930, 1124, 1136, 1154
HpySE526I ACGT 3 cut(s) 141, 1199, 1459
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 8 cut(s) 64, 275, 773, 782, 788, 1012, 1069, 1305
HspAI GCGC 1 cut(s) 1204
KroI GCCGGC 1 cut(s) 863
KroNI GCCGGC 1 cut(s) 865
Ksp22I TGATCA 1 cut(s) 63
KspI CCGCGG 1 cut(s) 121
Kzo9I GATC 7 cut(s) 63, 276, 641, 744, 1129, 1150, 1482
LmnI GCTCC 3 cut(s) 655, 931, 1582
Lsp1109I GCAGC 2 cut(s) 217, 337
LweI GCATC 4 cut(s) 379, 1045, 1150, 1502
MaeI CTAG 3 cut(s) 554, 962, 1476
MaeII ACGT 3 cut(s) 141, 1199, 1459
MaeIII GTNAC 2 cut(s) 547, 568
MalI GATC 7 cut(s) 65, 278, 643, 746, 1131, 1152, 1484
MboI GATC 7 cut(s) 63, 276, 641, 744, 1129, 1150, 1482
MboII GAAGA 8 cut(s) 49, 103, 106, 495, 596, 1112, 1124, 1577
MflI RGATCY 1 cut(s) 1129
MhlI GDGCHC 1 cut(s) 596
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 4 cut(s) 344, 579, 754, 1041
MluNI TGGCCA 1 cut(s) 902
MlyI GAGTC 2 cut(s) 389, 1431
Mox20I TGGCCA 1 cut(s) 902
Mph1103I ATGCAT 1 cut(s) 1060
MroNI GCCGGC 1 cut(s) 863
MscI TGGCCA 1 cut(s) 902
MseI TTAA 4 cut(s) 126, 540, 1079, 1368
MslI CAYNNNNRTG 1 cut(s) 783
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 3 cut(s) 84, 120, 189
MspI CCGG 9 cut(s) 176, 216, 225, 480, 864, 1083, 1239, 1277, 1562
MspR9I CCNGG 5 cut(s) 176, 330, 1035, 1277, 1562
Mva1269I GAATGC 2 cut(s) 153, 1058
MvaI CCWGG 2 cut(s) 330, 1035
MvnI CGCG 2 cut(s) 120, 267
MwoI GCNNNNNNNGC 6 cut(s) 221, 230, 236, 325, 675, 1326
NaeI GCCGGC 1 cut(s) 865
NciI CCSGG 3 cut(s) 176, 1277, 1562
NcoI CCATGG 1 cut(s) 778
NdeI CATATG 1 cut(s) 1331
NdeII GATC 7 cut(s) 63, 276, 641, 744, 1129, 1150, 1482
NgoMIV GCCGGC 1 cut(s) 863
NlaIII CATG 8 cut(s) 64, 275, 773, 782, 788, 1012, 1069, 1305
NlaIV GGNNCC 5 cut(s) 214, 595, 927, 936, 1243
NmuCI GTSAC 2 cut(s) 547, 568
NsbI TGCGCA 1 cut(s) 1205
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 5 cut(s) 275, 773, 788, 1012, 1305
PaeI GCATGC 1 cut(s) 1012
PagI TCATGA 1 cut(s) 60
PciI ACATGT 2 cut(s) 271, 784
PctI GAATGC 2 cut(s) 153, 1058
PdiI GCCGGC 1 cut(s) 865
PfeI GAWTC 5 cut(s) 113, 306, 1187, 1438, 1500
PkrI GCNGC 9 cut(s) 86, 122, 191, 232, 266, 327, 695, 967, 1430
PleI GAGTC 2 cut(s) 388, 1430
PpsI GAGTC 2 cut(s) 388, 1430
Ppu21I YACGTR 1 cut(s) 142
PscI ACATGT 2 cut(s) 271, 784
Psp6I CCWGG 2 cut(s) 328, 1033
PspFI CCCAGC 1 cut(s) 43
PspGI CCWGG 2 cut(s) 328, 1033
PspN4I GGNNCC 5 cut(s) 214, 595, 927, 936, 1243
PspPI GGNCC 1 cut(s) 1343
PsuI RGATCY 1 cut(s) 1129
RsaI GTAC 8 cut(s) 140, 270, 614, 706, 835, 876, 908, 1250
RsaNI GTAC 8 cut(s) 139, 269, 613, 705, 834, 875, 907, 1249
RseI CAYNNNNRTG 1 cut(s) 783
SacII CCGCGG 1 cut(s) 121
SaqAI TTAA 4 cut(s) 126, 540, 1079, 1368
SatI GCNGC 9 cut(s) 85, 121, 190, 231, 265, 326, 694, 966, 1429
Sau3AI GATC 7 cut(s) 63, 276, 641, 744, 1129, 1150, 1482
Sau96I GGNCC 1 cut(s) 1343
ScaI AGTACT 1 cut(s) 908
SchI GAGTC 2 cut(s) 389, 1431
ScrFI CCNGG 5 cut(s) 176, 330, 1035, 1277, 1562
SduI GDGCHC 1 cut(s) 596
SfaNI GCATC 4 cut(s) 379, 1045, 1150, 1502
Sfr303I CCGCGG 1 cut(s) 121
SgrBI CCGCGG 1 cut(s) 121
SinI GGWCC 1 cut(s) 1343
SmiMI CAYNNNNRTG 1 cut(s) 783
SmlI CTYRAG 2 cut(s) 289, 362
SmoI CTYRAG 2 cut(s) 289, 362
SphI GCATGC 1 cut(s) 1012
Sse9I AATT 4 cut(s) 344, 579, 754, 1041
SspMI CTAG 3 cut(s) 554, 962, 1476
StyD4I CCNGG 5 cut(s) 174, 328, 1033, 1275, 1560
StyI CCWWGG 3 cut(s) 453, 778, 1321
TaaI ACNGT 7 cut(s) 371, 444, 568, 598, 893, 919, 1099
TaiI ACGT 3 cut(s) 144, 1202, 1462
TaqI TCGA 4 cut(s) 105, 279, 972, 1308
TasI AATT 4 cut(s) 344, 579, 754, 1041
TatI WGTACW 4 cut(s) 612, 704, 906, 1248
TauI GCSGC 7 cut(s) 87, 123, 192, 267, 696, 968, 1431
TfiI GAWTC 5 cut(s) 113, 306, 1187, 1438, 1500
Tru1I TTAA 4 cut(s) 126, 540, 1079, 1368
Tru9I TTAA 4 cut(s) 126, 540, 1079, 1368
TscAI CASTG 2 cut(s) 660, 1162
TseFI GTSAC 2 cut(s) 547, 568
TseI GCWGC 2 cut(s) 230, 325
Tsp45I GTSAC 2 cut(s) 547, 568
TspDTI ATGAA 4 cut(s) 49, 496, 1206, 1326
TspGWI ACGGA 4 cut(s) 144, 209, 373, 1356
TspRI CASTG 2 cut(s) 660, 1162
VpaK11BI GGWCC 1 cut(s) 1343
XceI RCATGY 5 cut(s) 275, 773, 788, 1012, 1305
XcmI CCANNNNNNNNNTGG 1 cut(s) 17
XspI CTAG 3 cut(s) 554, 962, 1476
ZrmI AGTACT 1 cut(s) 908
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.