squalene
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 194 |
| AccII | CGCG | 1 cut(s) | 1110 |
| AciI | CCGC | 3 cut(s) | 948, 1108, 1366 |
| AclWI | GGATC | 3 cut(s) | 553, 966, 1205 |
| AcoI | YGGCCR | 4 cut(s) | 481, 882, 945, 1363 |
| AcuI | CTGAAG | 2 cut(s) | 846, 1482 |
| AfaI | GTAC | 5 cut(s) | 175, 843, 858, 932, 1497 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1016, 1064 |
| AflIII | ACRYGT | 1 cut(s) | 247 |
| AjiI | CACGTC | 1 cut(s) | 248 |
| AjnI | CCWGG | 3 cut(s) | 310, 844, 1015 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 616, 648 |
| AluBI | AGCT | 5 cut(s) | 215, 301, 619, 869, 928 |
| AluI | AGCT | 5 cut(s) | 215, 301, 619, 869, 928 |
| Alw26I | GTCTC | 3 cut(s) | 261, 347, 774 |
| AlwI | GGATC | 3 cut(s) | 553, 966, 1205 |
| Ama87I | CYCGRG | 1 cut(s) | 344 |
| AoxI | GGCC | 4 cut(s) | 481, 882, 945, 1363 |
| ApeKI | GCWGC | 5 cut(s) | 212, 307, 657, 689, 995 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 98 |
| AspLEI | GCGC | 1 cut(s) | 1398 |
| AspS9I | GGNCC | 1 cut(s) | 1280 |
| AsuHPI | GGTGA | 3 cut(s) | 887, 1197, 1380 |
| AvaI | CYCGRG | 1 cut(s) | 344 |
| AvaII | GGWCC | 1 cut(s) | 1280 |
| AxyI | CCTNAGG | 1 cut(s) | 912 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 840, 873 |
| BalI | TGGCCA | 1 cut(s) | 884 |
| BanI | GGYRCC | 1 cut(s) | 194 |
| BanII | GRGCYC | 1 cut(s) | 53 |
| BbvI | GCAGC | 5 cut(s) | 224, 319, 644, 676, 982 |
| BccI | CCATC | 4 cut(s) | 401, 647, 800, 1358 |
| BceAI | ACGGC | 1 cut(s) | 468 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 335, 369 |
| BciT130I | CCWGG | 3 cut(s) | 312, 846, 1017 |
| BciVI | GTATCC | 1 cut(s) | 409 |
| BclI | TGATCA | 1 cut(s) | 66 |
| BcoDI | GTCTC | 3 cut(s) | 261, 347, 774 |
| BfaI | CTAG | 3 cut(s) | 870, 1011, 1166 |
| BfmI | CTRYAG | 2 cut(s) | 305, 1507 |
| BfuI | GTATCC | 1 cut(s) | 409 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1263 |
| BglII | AGATCT | 1 cut(s) | 1511 |
| BisI | GCNGC | 7 cut(s) | 213, 308, 658, 690, 948, 996, 1366 |
| BlsI | GCNGC | 7 cut(s) | 214, 309, 659, 691, 949, 997, 1367 |
| BmcAI | AGTACT | 1 cut(s) | 175 |
| Bme1390I | CCNGG | 3 cut(s) | 312, 846, 1017 |
| Bme18I | GGWCC | 1 cut(s) | 1280 |
| BmeT110I | CYCGRG | 1 cut(s) | 344 |
| BmgBI | CACGTC | 1 cut(s) | 248 |
| BmgT120I | GGNCC | 1 cut(s) | 1280 |
| BmiI | GGNNCC | 3 cut(s) | 196, 909, 918 |
| BmrFI | CCNGG | 3 cut(s) | 312, 846, 1017 |
| BmsI | GCATC | 7 cut(s) | 361, 1063, 1132, 1211, 1275, 1439, 1446 |
| BpmI | CTGGAG | 1 cut(s) | 630 |
| BpuEI | CTTGAG | 2 cut(s) | 292, 1444 |
| BsaI | GGTCTC | 2 cut(s) | 347, 774 |
| BsaJI | CCNNGG | 4 cut(s) | 234, 311, 819, 1258 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 616, 646 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1016, 1064 |
| Bse118I | RCCGGY | 2 cut(s) | 206, 1147 |
| Bse1I | ACTGG | 1 cut(s) | 1283 |
| Bse21I | CCTNAGG | 1 cut(s) | 912 |
| BseBI | CCWGG | 3 cut(s) | 312, 846, 1017 |
| BseDI | CCNNGG | 4 cut(s) | 234, 311, 819, 1258 |
| BseGI | GGATG | 3 cut(s) | 792, 844, 1054 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1016, 1064 |
| BseMII | CTCAG | 3 cut(s) | 611, 697, 926 |
| BseNI | ACTGG | 1 cut(s) | 1283 |
| BseRI | GAGGAG | 4 cut(s) | 629, 900, 1083, 1506 |
| BseX3I | CGGCCG | 1 cut(s) | 1363 |
| BseXI | GCAGC | 5 cut(s) | 224, 319, 644, 676, 982 |
| BseYI | CCCAGC | 2 cut(s) | 46, 461 |
| BsgI | GTGCAG | 1 cut(s) | 231 |
| Bsh1236I | CGCG | 1 cut(s) | 1110 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1366 |
| BshFI | GGCC | 4 cut(s) | 483, 884, 947, 1365 |
| BshNI | GGYRCC | 1 cut(s) | 194 |
| BsiEI | CGRYCG | 1 cut(s) | 1366 |
| BsiHKCI | CYCGRG | 1 cut(s) | 344 |
| BsiSI | CCGG | 4 cut(s) | 198, 207, 1148, 1214 |
| BslFI | GGGAC | 1 cut(s) | 690 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1016, 1064 |
| BsmAI | GTCTC | 3 cut(s) | 261, 347, 774 |
| BsmFI | GGGAC | 1 cut(s) | 690 |
| BsmI | GAATGC | 1 cut(s) | 504 |
| BsnI | GGCC | 4 cut(s) | 483, 884, 947, 1365 |
| Bso31I | GGTCTC | 2 cut(s) | 347, 774 |
| BsoBI | CYCGRG | 1 cut(s) | 344 |
| Bsp1286I | GDGCHC | 1 cut(s) | 53 |
| Bsp1407I | TGTACA | 1 cut(s) | 930 |
| BspACI | CCGC | 3 cut(s) | 948, 1108, 1366 |
| BspANI | GGCC | 4 cut(s) | 483, 884, 947, 1365 |
| BspCNI | CTCAG | 3 cut(s) | 612, 696, 925 |
| BspFNI | CGCG | 1 cut(s) | 1110 |
| BspHI | TCATGA | 1 cut(s) | 63 |
| BspLI | GGNNCC | 3 cut(s) | 196, 909, 918 |
| BspMAI | CTGCAG | 1 cut(s) | 309 |
| BspPI | GGATC | 3 cut(s) | 553, 966, 1205 |
| BspT107I | GGYRCC | 1 cut(s) | 194 |
| BspTNI | GGTCTC | 2 cut(s) | 347, 774 |
| BsrFI | RCCGGY | 2 cut(s) | 206, 1147 |
| BsrGI | TGTACA | 1 cut(s) | 930 |
| BsrI | ACTGG | 1 cut(s) | 1283 |
| BssAI | RCCGGY | 2 cut(s) | 206, 1147 |
| BssECI | CCNNGG | 4 cut(s) | 234, 311, 819, 1258 |
| BssT1I | CCWWGG | 2 cut(s) | 234, 1258 |
| Bst2UI | CCWGG | 3 cut(s) | 312, 846, 1017 |
| Bst4CI | ACNGT | 8 cut(s) | 143, 426, 449, 643, 901, 1081, 1374, 1495 |
| Bst6I | CTCTTC | 2 cut(s) | 1011, 1296 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 218 |
| BstAUI | TGTACA | 1 cut(s) | 930 |
| BstDEI | CTNAG | 4 cut(s) | 620, 683, 912, 1118 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1062 |
| BstF5I | GGATG | 3 cut(s) | 792, 844, 1054 |
| BstFNI | CGCG | 1 cut(s) | 1110 |
| BstHHI | GCGC | 1 cut(s) | 1398 |
| BstMAI | GTCTC | 3 cut(s) | 261, 347, 774 |
| BstMCI | CGRYCG | 1 cut(s) | 1366 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 203, 212, 218, 307, 944, 1254, 1263, 1402 |
| BstNI | CCWGG | 3 cut(s) | 312, 846, 1017 |
| BstNSI | RCATGY | 3 cut(s) | 257, 770, 1402 |
| BstSCI | CCNGG | 3 cut(s) | 310, 844, 1015 |
| BstSFI | CTRYAG | 2 cut(s) | 305, 1507 |
| BstUI | CGCG | 1 cut(s) | 1110 |
| BstV1I | GCAGC | 5 cut(s) | 224, 319, 644, 676, 982 |
| BstX2I | RGATCY | 2 cut(s) | 971, 1511 |
| BstYI | RGATCY | 2 cut(s) | 971, 1511 |
| BstZI | CGGCCG | 1 cut(s) | 1363 |
| Bsu36I | CCTNAGG | 1 cut(s) | 912 |
| BsuI | GTATCC | 1 cut(s) | 409 |
| BsuRI | GGCC | 4 cut(s) | 483, 884, 947, 1365 |
| BtrI | CACGTC | 1 cut(s) | 248 |
| BtsCI | GGATG | 3 cut(s) | 792, 844, 1054 |
| BtsI | GCAGTG | 1 cut(s) | 1181 |
| BtsIMutI | CAGTG | 5 cut(s) | 422, 555, 691, 906, 1181 |
| CciI | TCATGA | 1 cut(s) | 63 |
| CfoI | GCGC | 1 cut(s) | 1398 |
| Cfr10I | RCCGGY | 2 cut(s) | 206, 1147 |
| Cfr13I | GGNCC | 1 cut(s) | 1280 |
| Csp6I | GTAC | 5 cut(s) | 174, 842, 857, 931, 1496 |
| CviAII | CATG | 9 cut(s) | 64, 254, 761, 767, 976, 991, 1048, 1130, 1399 |
| CviQI | GTAC | 5 cut(s) | 174, 842, 857, 931, 1496 |
| DdeI | CTNAG | 4 cut(s) | 620, 683, 912, 1118 |
| EaeI | YGGCCR | 4 cut(s) | 481, 882, 945, 1363 |
| EagI | CGGCCG | 1 cut(s) | 1363 |
| Eam1104I | CTCTTC | 2 cut(s) | 1011, 1296 |
| EarI | CTCTTC | 2 cut(s) | 1011, 1296 |
| EclXI | CGGCCG | 1 cut(s) | 1363 |
| Eco130I | CCWWGG | 2 cut(s) | 234, 1258 |
| Eco24I | GRGCYC | 1 cut(s) | 53 |
| Eco31I | GGTCTC | 2 cut(s) | 347, 774 |
| Eco32I | GATATC | 2 cut(s) | 181, 320 |
| Eco47I | GGWCC | 1 cut(s) | 1280 |
| Eco52I | CGGCCG | 1 cut(s) | 1363 |
| Eco57I | CTGAAG | 2 cut(s) | 846, 1482 |
| Eco81I | CCTNAGG | 1 cut(s) | 912 |
| Eco88I | CYCGRG | 1 cut(s) | 344 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1062 |
| EcoRII | CCWGG | 3 cut(s) | 310, 844, 1015 |
| EcoRV | GATATC | 2 cut(s) | 181, 320 |
| EcoT14I | CCWWGG | 2 cut(s) | 234, 1258 |
| EcoT38I | GRGCYC | 1 cut(s) | 53 |
| ErhI | CCWWGG | 2 cut(s) | 234, 1258 |
| FaeI | CATG | 9 cut(s) | 67, 257, 764, 770, 979, 994, 1051, 1133, 1402 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 694, 726 |
| FaqI | GGGAC | 1 cut(s) | 690 |
| FatI | CATG | 9 cut(s) | 63, 253, 760, 766, 975, 990, 1047, 1129, 1398 |
| FauNDI | CATATG | 1 cut(s) | 628 |
| FbaI | TGATCA | 1 cut(s) | 66 |
| Fnu4HI | GCNGC | 7 cut(s) | 213, 308, 658, 690, 948, 996, 1366 |
| FokI | GGATG | 3 cut(s) | 779, 851, 1041 |
| FriOI | GRGCYC | 1 cut(s) | 53 |
| Fsp4HI | GCNGC | 7 cut(s) | 213, 308, 658, 690, 948, 996, 1366 |
| FspBI | CTAG | 3 cut(s) | 870, 1011, 1166 |
| GlaI | GCGC | 1 cut(s) | 1397 |
| GluI | GCNGC | 7 cut(s) | 213, 308, 658, 690, 948, 996, 1366 |
| GsaI | CCCAGC | 2 cut(s) | 50, 465 |
| GsuI | CTGGAG | 1 cut(s) | 630 |
| HaeIII | GGCC | 4 cut(s) | 483, 884, 947, 1365 |
| HapII | CCGG | 4 cut(s) | 198, 207, 1148, 1214 |
| HhaI | GCGC | 1 cut(s) | 1398 |
| Hin1II | CATG | 9 cut(s) | 67, 257, 764, 770, 979, 994, 1051, 1133, 1402 |
| Hin6I | GCGC | 1 cut(s) | 1396 |
| HinP1I | GCGC | 1 cut(s) | 1396 |
| HinfI | GANTC | 3 cut(s) | 362, 1037, 1169 |
| HpaII | CCGG | 4 cut(s) | 198, 207, 1148, 1214 |
| HphI | GGTGA | 3 cut(s) | 887, 1197, 1380 |
| Hpy188I | TCNGA | 3 cut(s) | 137, 454, 1093 |
| Hpy188III | TCNNGA | 4 cut(s) | 64, 344, 1136, 1423 |
| Hpy99I | CGWCG | 1 cut(s) | 249 |
| HpyAV | CCTTC | 6 cut(s) | 233, 406, 795, 870, 1395, 1457 |
| HpyCH4III | ACNGT | 8 cut(s) | 143, 426, 449, 643, 901, 1081, 1374, 1495 |
| HpyCH4IV | ACGT | 1 cut(s) | 247 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 203, 212, 218, 307, 944, 1254, 1263, 1402 |
| HpyF3I | CTNAG | 4 cut(s) | 620, 683, 912, 1118 |
| HpySE526I | ACGT | 1 cut(s) | 247 |
| Hsp92II | CATG | 9 cut(s) | 67, 257, 764, 770, 979, 994, 1051, 1133, 1402 |
| HspAI | GCGC | 1 cut(s) | 1396 |
| Ksp22I | TGATCA | 1 cut(s) | 66 |
| LmnI | GCTCC | 5 cut(s) | 298, 616, 637, 913, 1277 |
| Lsp1109I | GCAGC | 5 cut(s) | 224, 319, 644, 676, 982 |
| LweI | GCATC | 7 cut(s) | 361, 1063, 1132, 1211, 1275, 1439, 1446 |
| MaeI | CTAG | 3 cut(s) | 870, 1011, 1166 |
| MaeII | ACGT | 1 cut(s) | 247 |
| MaeIII | GTNAC | 1 cut(s) | 550 |
| MboII | GAAGA | 6 cut(s) | 52, 737, 998, 1094, 1106, 1283 |
| MfeI | CAATTG | 2 cut(s) | 366, 1415 |
| MflI | RGATCY | 2 cut(s) | 971, 1511 |
| MhlI | GDGCHC | 1 cut(s) | 53 |
| MlsI | TGGCCA | 1 cut(s) | 884 |
| MluNI | TGGCCA | 1 cut(s) | 884 |
| MlyI | GAGTC | 1 cut(s) | 371 |
| Mox20I | TGGCCA | 1 cut(s) | 884 |
| MroXI | GAANNNNTTC | 1 cut(s) | 98 |
| MscI | TGGCCA | 1 cut(s) | 884 |
| MseI | TTAA | 3 cut(s) | 1061, 1305, 1528 |
| MslI | CAYNNNNRTG | 1 cut(s) | 765 |
| Msp20I | TGGCCA | 1 cut(s) | 884 |
| MspA1I | CMGCKG | 1 cut(s) | 928 |
| MspI | CCGG | 4 cut(s) | 198, 207, 1148, 1214 |
| MspR9I | CCNGG | 3 cut(s) | 312, 846, 1017 |
| MunI | CAATTG | 2 cut(s) | 366, 1415 |
| Mva1269I | GAATGC | 1 cut(s) | 504 |
| MvaI | CCWGG | 3 cut(s) | 312, 846, 1017 |
| MvnI | CGCG | 1 cut(s) | 1110 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 203, 212, 218, 307, 944, 1254, 1263, 1402 |
| NdeI | CATATG | 1 cut(s) | 628 |
| NlaIII | CATG | 9 cut(s) | 67, 257, 764, 770, 979, 994, 1051, 1133, 1402 |
| NlaIV | GGNNCC | 3 cut(s) | 196, 909, 918 |
| NmuCI | GTSAC | 1 cut(s) | 550 |
| NspI | RCATGY | 3 cut(s) | 257, 770, 1402 |
| PaeR7I | CTCGAG | 1 cut(s) | 344 |
| PagI | TCATGA | 1 cut(s) | 63 |
| PctI | GAATGC | 1 cut(s) | 504 |
| PdmI | GAANNNNTTC | 1 cut(s) | 98 |
| PfeI | GAWTC | 2 cut(s) | 1037, 1169 |
| PkrI | GCNGC | 7 cut(s) | 214, 309, 659, 691, 949, 997, 1367 |
| PleI | GAGTC | 1 cut(s) | 370 |
| PpsI | GAGTC | 1 cut(s) | 370 |
| Psp6I | CCWGG | 3 cut(s) | 310, 844, 1015 |
| PspFI | CCCAGC | 2 cut(s) | 46, 461 |
| PspGI | CCWGG | 3 cut(s) | 310, 844, 1015 |
| PspN4I | GGNNCC | 3 cut(s) | 196, 909, 918 |
| PspPI | GGNCC | 1 cut(s) | 1280 |
| PstI | CTGCAG | 1 cut(s) | 309 |
| PsuI | RGATCY | 2 cut(s) | 971, 1511 |
| PvuII | CAGCTG | 1 cut(s) | 928 |
| RsaI | GTAC | 5 cut(s) | 175, 843, 858, 932, 1497 |
| RsaNI | GTAC | 5 cut(s) | 174, 842, 857, 931, 1496 |
| RseI | CAYNNNNRTG | 1 cut(s) | 765 |
| SaqAI | TTAA | 3 cut(s) | 1061, 1305, 1528 |
| SatI | GCNGC | 7 cut(s) | 213, 308, 658, 690, 948, 996, 1366 |
| Sau96I | GGNCC | 1 cut(s) | 1280 |
| ScaI | AGTACT | 1 cut(s) | 175 |
| SchI | GAGTC | 1 cut(s) | 371 |
| ScrFI | CCNGG | 3 cut(s) | 312, 846, 1017 |
| SduI | GDGCHC | 1 cut(s) | 53 |
| SfaNI | GCATC | 7 cut(s) | 361, 1063, 1132, 1211, 1275, 1439, 1446 |
| SfcI | CTRYAG | 2 cut(s) | 305, 1507 |
| Sfr274I | CTCGAG | 1 cut(s) | 344 |
| SinI | GGWCC | 1 cut(s) | 1280 |
| SlaI | CTCGAG | 1 cut(s) | 344 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 765 |
| SmlI | CTYRAG | 3 cut(s) | 271, 344, 1423 |
| SmoI | CTYRAG | 3 cut(s) | 271, 344, 1423 |
| SsiI | CCGC | 3 cut(s) | 948, 1108, 1366 |
| SspMI | CTAG | 3 cut(s) | 870, 1011, 1166 |
| StyD4I | CCNGG | 3 cut(s) | 310, 844, 1015 |
| StyI | CCWWGG | 2 cut(s) | 234, 1258 |
| TaaI | ACNGT | 8 cut(s) | 143, 426, 449, 643, 901, 1081, 1374, 1495 |
| TaiI | ACGT | 1 cut(s) | 250 |
| TaqI | TCGA | 2 cut(s) | 345, 564 |
| TatI | WGTACW | 2 cut(s) | 173, 930 |
| TauI | GCSGC | 2 cut(s) | 950, 1368 |
| TfiI | GAWTC | 2 cut(s) | 1037, 1169 |
| Tru1I | TTAA | 3 cut(s) | 1061, 1305, 1528 |
| Tru9I | TTAA | 3 cut(s) | 1061, 1305, 1528 |
| TscAI | CASTG | 5 cut(s) | 429, 555, 691, 906, 1188 |
| TseFI | GTSAC | 1 cut(s) | 550 |
| TseI | GCWGC | 5 cut(s) | 212, 307, 657, 689, 995 |
| Tsp45I | GTSAC | 1 cut(s) | 550 |
| TspDTI | ATGAA | 3 cut(s) | 52, 1029, 1514 |
| TspRI | CASTG | 5 cut(s) | 429, 555, 691, 906, 1188 |
| VpaK11BI | GGWCC | 1 cut(s) | 1280 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1062 |
| XceI | RCATGY | 3 cut(s) | 257, 770, 1402 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 383 |
| XhoI | CTCGAG | 1 cut(s) | 344 |
| XmnI | GAANNNNTTC | 1 cut(s) | 98 |
| XspI | CTAG | 3 cut(s) | 870, 1011, 1166 |
| ZrmI | AGTACT | 1 cut(s) | 175 |