RchiOBHm_Chr6g0246811

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000108
Physical Location & Seq
Forward (+)
2533824 .. 2534417
594 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22124

Sequence Viewer

Length: 357 bp
ATGGTTTCGTACGAGTATGTTTTGGCTGGGGTGTTAGTTTCTTTGCTGGGCTCTATTTTCTTCATGATCATCAGCTCGATTTTGGGAGGAAAGAAACAATTCGGAAACGGTGAGTTTCTGCCGGAAATGGAGGAGGAGAAAACTACCGATGTCATCATTGTTGGTGCCGGAGTTGCTGGTGCAGCTCTTGCTTACACTCTTGCCAAGGAAGGACGGCGTGTGCATGTAATTGAAAGAGACTTAAGTGAGCCAGACAGAATTGTTGGGGAGCTGTTGCAGCCTGGGGGATATCTCAAATTGATTGAGTTGGGTCGTGAAGGTAAGCAATCACCTAATCTAGAATTGTGTGCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

118

Amino Acids

12.74

Weight (kDa)

4.86

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thi4 PF01946 43 - 82 1.3e-06 Thi4 family
FAD_binding_3 PF01494 49 - 80 2.4e-06 FAD binding domain
DAO PF01266 50 - 80 6.1e-09 FAD dependent oxidoreductase
FAD_binding_2 PF00890 50 - 79 9e-07 FAD binding domain
NAD_binding_8 PF13450 53 - 79 2.5e-07 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AfaI GTAC 1 cut(s) 11
AflII CTTAAG 1 cut(s) 241
AgsI TTSAA 1 cut(s) 233
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 3 cut(s) 75, 185, 271
AluI AGCT 3 cut(s) 75, 185, 271
Alw26I GTCTC 1 cut(s) 231
ApeKI GCWGC 2 cut(s) 182, 277
Asp700I GAANNNNTTC 1 cut(s) 98
AsuHPI GGTGA 2 cut(s) 122, 321
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 194, 289
BceAI ACGGC 1 cut(s) 230
BciT130I CCWGG 1 cut(s) 282
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 1 cut(s) 338
BfrI CTTAAG 1 cut(s) 241
BisI GCNGC 2 cut(s) 183, 278
BlsI GCNGC 2 cut(s) 184, 279
Bme1390I CCNGG 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 204, 281
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 2 cut(s) 204, 281
BseRI GAGGAG 2 cut(s) 146, 149
BseXI GCAGC 2 cut(s) 194, 289
BseYI CCCAGC 2 cut(s) 26, 46
BsgI GTGCAG 1 cut(s) 201
BshNI GGYRCC 1 cut(s) 164
BsiSI CCGG 2 cut(s) 122, 168
BsiWI CGTACG 1 cut(s) 9
BsmAI GTCTC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 66
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 164
BspTI CTTAAG 1 cut(s) 241
BssECI CCNNGG 2 cut(s) 204, 281
BssMI GATC 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 1 cut(s) 110
BstAFI CTTAAG 1 cut(s) 241
BstAPI GCANNNNNTGC 1 cut(s) 188
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 4 cut(s) 173, 182, 188, 277
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 280
BstV1I GCAGC 2 cut(s) 194, 289
CciI TCATGA 1 cut(s) 63
Csp6I GTAC 1 cut(s) 10
CviAII CATG 2 cut(s) 64, 224
CviJI RGCY 7 cut(s) 26, 51, 75, 185, 250, 271, 280
CviKI_1 RGCY 7 cut(s) 26, 51, 75, 185, 250, 271, 280
CviQI GTAC 1 cut(s) 10
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 1 cut(s) 53
Eco32I GATATC 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 280
EcoRV GATATC 1 cut(s) 290
EcoT14I CCWWGG 1 cut(s) 204
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 2 cut(s) 67, 227
FaiI YATR 3 cut(s) 18, 65, 225
FatI CATG 2 cut(s) 63, 223
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 183, 278
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 183, 278
FspBI CTAG 1 cut(s) 338
GluI GCNGC 2 cut(s) 183, 278
GsaI CCCAGC 2 cut(s) 30, 50
HapII CCGG 2 cut(s) 122, 168
Hin1II CATG 2 cut(s) 67, 227
HpaII CCGG 2 cut(s) 122, 168
HphI GGTGA 2 cut(s) 122, 321
Hpy188I TCNGA 1 cut(s) 104
Hpy188III TCNNGA 3 cut(s) 64, 314, 338
HpyAV CCTTC 2 cut(s) 203, 311
HpyCH4III ACNGT 1 cut(s) 110
HpyCH4V TGCA 4 cut(s) 182, 223, 277, 350
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 182, 188, 277
Hsp92II CATG 2 cut(s) 67, 227
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 8 cut(s) 12, 32, 135, 162, 181, 264, 267, 294
Lsp1109I GCAGC 2 cut(s) 194, 289
MaeI CTAG 1 cut(s) 338
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MboII GAAGA 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 5 cut(s) 98, 228, 258, 296, 341
MnlI CCTC 3 cut(s) 80, 124, 127
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 1 cut(s) 242
MspCI CTTAAG 1 cut(s) 241
MspI CCGG 2 cut(s) 122, 168
MspR9I CCNGG 1 cut(s) 282
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 4 cut(s) 173, 182, 188, 277
NdeII GATC 1 cut(s) 66
NlaIII CATG 2 cut(s) 67, 227
NlaIV GGNNCC 1 cut(s) 166
NspI RCATGY 1 cut(s) 227
PagI TCATGA 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 98
Pfl23II CGTACG 1 cut(s) 9
PkrI GCNGC 2 cut(s) 184, 279
Psp6I CCWGG 1 cut(s) 280
PspFI CCCAGC 2 cut(s) 26, 46
PspGI CCWGG 1 cut(s) 280
PspLI CGTACG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 166
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SaqAI TTAA 1 cut(s) 242
SatI GCNGC 2 cut(s) 183, 278
Sau3AI GATC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 1 cut(s) 53
SetI ASST 5 cut(s) 77, 187, 273, 322, 334
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 5 cut(s) 98, 228, 258, 296, 341
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 1 cut(s) 110
TaqI TCGA 1 cut(s) 77
TasI AATT 5 cut(s) 98, 228, 258, 296, 341
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TseI GCWGC 2 cut(s) 182, 277
TspDTI ATGAA 1 cut(s) 52
Vha464I CTTAAG 1 cut(s) 241
XbaI TCTAGA 1 cut(s) 337
XceI RCATGY 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.