Rroxscaffold_7G00214460

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000108
Physical Location & Seq
Reverse (-)
65104016 .. 65107183
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214460.1

Sequence Viewer

Length: 1572 bp
ATGGTGTCTTACGAGTACGTTCTGGGTGGAGTCTTGGCTTCTCTGCTCGGCTCTGTTTTCCTCATGATCATCTGCAGCACTTTTAGCGCCAAGAAAAAGGTGGAGGATTTGAAAACTAATAATGGGTTTGTGAGGATGCCGCAGAACGGTGCTTTTCGGCCGGAGATGGAGGAGGAGGAGAAAAGTACCGACGTCGTCATTGTCGGCGCCGGAGTTGCCGGTGCAGCTCTTGCTTACACTCTTGCCAAGGAAGGACGGCGTGTACATGTTATTGAAAGAGACTTGAGTGAGCCAGACAGAATTGTTGGTGAGCTTTTGCAGCCTGGGTATCTATTTGTGGCTGCAGATTGTGCAAACGAGTCAATTGATGCTCAGAAGGTGTTTGGTTATGCTCTTTACAAAGATGGCAAGGATACAAAACTATCTTATCCCTTGGAGCAGTATAGTTCAGATGTGGCTGGGAGAAGTTTCCACAATGGGCGTTTTATTCAAAGAATGCGTGAAAAAGCTACAACTCTTTCAAATGTCAAATTGGAACAAGGATCGGTGACAACACTAATCGAGGAAAAGGGCATTGTCAAAGGGGTGATTTACAAGAACAAGGCTGGAGAGGAGATGAGAACATATGCTCCACTAACAATCGTGTGCGATGGCTGCTTTTCAAATCTGCGCAAATCTCTCAGTGATCCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGGTGTTAGAGAACTGTGATCTTCCACATGCAAATCATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTGTTTTACCCTATTAGTTCCACTGAGATTCGTTGTTTGGTCGATGTACCTGGGACAAAAGTACCTTCAGTTGCTAGTGGTGAAATGGCTAACTATTTGAAGACTTTGGTGGCTCCTCAGGTTCCCCCACAGCTGTACAATGCTTTTATGGTTGCAATAGAGAAAGGAAACATTAGATCCATGCAAAACAAAAGCATGGCTGCTAATCCTGTTCCCACTCCTGGTGCAATTTTGTTGGGGGATTCATTCAATATGAGGCATCCTTTAACAGGAGGAGGAATGACCGTGGCTCTTTCAGACATAGTTCTTCTCCGCGATCTTCTTAGACCCCTACGTGATCTCAATGATGCACCTGCTTTGTGCAATTACCTTGAATCATTTTATACATTGCGTAAGCCTGTGTCATCTACTATAAACACATTGGCAGGTGCTTTGTACAAGGTGTTTTGTGCATCGCCTGATCCAGCAAGACAGGAAATGCGTGAAGCATGTTTTGGCTATTTGAGCCTTGGAGGAATCTGTTCATATGGACCAGTATCTCTGCTCTCTGGCCTTAACCCTCGTCCGCTACACTTGTTCCTCCATTTCTTTGCAGTTGCTATCTATGGTGTTGGGCGCTTAATGCTTCCATTCCCTTCACCTCAACGCATCTGGCTTGGGTTTAGATTGATCTTAAGTGCATCAGGCATCATATTCCCCATTATAAAGGGAGAAGGAGTTAGACAGATGTTCTTTCCAGCAACGGTGCCAGCATATTATAGATCACCACCTGTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

523

Amino Acids

57.15

Weight (kDa)

8.36

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 63 - 369 1.2e-13 FAD binding domain
DAO PF01266 64 - 94 1.9e-07 FAD dependent oxidoreductase
FAD_binding_2 PF00890 64 - 93 1e-05 FAD binding domain
NAD_binding_8 PF13450 67 - 93 2e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 210 - 482 6.9e-118 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1496
AarI CACCTGC 2 cut(s) 1153, 1208
AatII GACGTC 1 cut(s) 195
Acc16I TGCGCA 1 cut(s) 671
Acc36I ACCTGC 2 cut(s) 1153, 1208
AccB1I GGYRCC 2 cut(s) 206, 1537
AccII CGCG 1 cut(s) 1107
AciI CCGC 3 cut(s) 140, 1105, 1358
AclWI GGATC 4 cut(s) 550, 680, 963, 1247
AcoI YGGCCR 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 843
AcyI GRCGYC 2 cut(s) 192, 207
AfaI GTAC 7 cut(s) 17, 187, 264, 840, 855, 929, 1229
AfiI CCNNNNNNNGG 3 cut(s) 146, 1013, 1061
AflII CTTAAG 1 cut(s) 1465
AflIII ACRYGT 2 cut(s) 265, 763
AgsI TTSAA 9 cut(s) 112, 275, 491, 522, 663, 698, 892, 1042, 1166
AjnI CCWGG 3 cut(s) 322, 841, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 1269, 1301
AloI GAACNNNNNNTCC 2 cut(s) 613, 645
AluBI AGCT 4 cut(s) 227, 313, 509, 925
AluI AGCT 4 cut(s) 227, 313, 509, 925
Alw26I GTCTC 2 cut(s) 273, 771
AlwI GGATC 4 cut(s) 550, 680, 963, 1247
AoxI GGCC 2 cut(s) 158, 1342
ApeKI GCWGC 6 cut(s) 75, 224, 319, 341, 654, 992
Asp700I GAANNNNTTC 1 cut(s) 1312
AspLEI GCGC 4 cut(s) 89, 209, 672, 1410
AspS9I GGNCC 1 cut(s) 1322
AsuHPI GGTGA 7 cut(s) 320, 559, 598, 777, 884, 1422, 1548
AvaII GGWCC 1 cut(s) 1322
AxyI CCTNAGG 1 cut(s) 909
BaeI ACNNNNGTAYC 2 cut(s) 837, 870
BanI GGYRCC 2 cut(s) 206, 1537
BbsI GAAGAC 1 cut(s) 899
BbvI GCAGC 6 cut(s) 87, 236, 328, 331, 641, 979
BccI CCATC 4 cut(s) 160, 398, 644, 797
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 3 cut(s) 324, 843, 1014
BciVI GTATCC 1 cut(s) 406
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 273, 771
BfaI CTAG 1 cut(s) 867
BfmI CTRYAG 2 cut(s) 73, 342
BfoI RGCGCY 3 cut(s) 90, 210, 1411
BfrI CTTAAG 1 cut(s) 1465
BfuAI ACCTGC 2 cut(s) 1153, 1208
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 7 cut(s) 76, 140, 225, 320, 342, 655, 993
BlsI GCNGC 7 cut(s) 77, 141, 226, 321, 343, 656, 994
Bme1390I CCNGG 3 cut(s) 324, 843, 1014
Bme18I GGWCC 1 cut(s) 1322
BmgT120I GGNCC 1 cut(s) 1322
BmiI GGNNCC 4 cut(s) 208, 906, 915, 1539
BmrFI CCNGG 3 cut(s) 324, 843, 1014
BmsI GCATC 8 cut(s) 126, 358, 1060, 1129, 1253, 1449, 1481, 1488
BpiI GAAGAC 1 cut(s) 899
BpmI CTGGAG 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 304
BsaAI YACGTR 1 cut(s) 1127
BsaHI GRCGYC 2 cut(s) 192, 207
BsaI GGTCTC 1 cut(s) 771
BsaJI CCNNGG 6 cut(s) 246, 323, 432, 842, 1077, 1300
BsaXI ACNNNNNCTCC 4 cut(s) 613, 643, 1494, 1524
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 1013, 1061
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 1 cut(s) 1325
Bse21I CCTNAGG 1 cut(s) 909
Bse3DI GCAATG 1 cut(s) 1178
BseBI CCWGG 3 cut(s) 324, 843, 1014
BseDI CCNNGG 6 cut(s) 246, 323, 432, 842, 1077, 1300
BseGI GGATG 3 cut(s) 141, 789, 1051
BseLI CCNNNNNNNGG 3 cut(s) 146, 1013, 1061
BseMI GCAATG 1 cut(s) 1178
BseMII CTCAG 4 cut(s) 386, 694, 807, 923
BseNI ACTGG 1 cut(s) 1325
BseRI GAGGAG 6 cut(s) 185, 188, 191, 626, 897, 1080
BseX3I CGGCCG 1 cut(s) 158
BseXI GCAGC 6 cut(s) 87, 236, 328, 331, 641, 979
BseYI CCCAGC 1 cut(s) 458
BsgI GTGCAG 1 cut(s) 243
Bsh1236I CGCG 1 cut(s) 1107
Bsh1285I CGRYCG 1 cut(s) 161
BshFI GGCC 2 cut(s) 160, 1344
BshNI GGYRCC 2 cut(s) 206, 1537
BsiEI CGRYCG 1 cut(s) 161
BsiSI CCGG 3 cut(s) 161, 210, 219
BslFI GGGAC 2 cut(s) 687, 859
BslI CCNNNNNNNGG 3 cut(s) 146, 1013, 1061
BsmAI GTCTC 2 cut(s) 273, 771
BsmFI GGGAC 2 cut(s) 687, 859
BsmI GAATGC 1 cut(s) 501
BsnI GGCC 2 cut(s) 160, 1344
Bso31I GGTCTC 1 cut(s) 771
Bsp1407I TGTACA 3 cut(s) 262, 927, 1227
BspACI CCGC 3 cut(s) 140, 1105, 1358
BspANI GGCC 2 cut(s) 160, 1344
BspCNI CTCAG 4 cut(s) 385, 693, 808, 922
BspFNI CGCG 1 cut(s) 1107
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 4 cut(s) 208, 906, 915, 1539
BspMAI CTGCAG 2 cut(s) 77, 346
BspMI ACCTGC 2 cut(s) 1153, 1208
BspPI GGATC 4 cut(s) 550, 680, 963, 1247
BspT107I GGYRCC 2 cut(s) 206, 1537
BspTI CTTAAG 1 cut(s) 1465
BspTNI GGTCTC 1 cut(s) 771
BsrDI GCAATG 1 cut(s) 1178
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 3 cut(s) 262, 927, 1227
BsrI ACTGG 1 cut(s) 1325
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 6 cut(s) 246, 323, 432, 842, 1077, 1300
BssNI GRCGYC 2 cut(s) 192, 207
BssT1I CCWWGG 3 cut(s) 246, 432, 1300
Bst2UI CCWGG 3 cut(s) 324, 843, 1014
Bst4CI ACNGT 4 cut(s) 149, 737, 1078, 1537
BstACI GRCGYC 2 cut(s) 192, 207
BstAFI CTTAAG 1 cut(s) 1465
BstAPI GCANNNNNTGC 2 cut(s) 230, 350
BstAUI TGTACA 3 cut(s) 262, 927, 1227
BstBAI YACGTR 1 cut(s) 1127
BstC8I GCNNGC 1 cut(s) 1542
BstDEI CTNAG 5 cut(s) 372, 680, 816, 909, 1115
BstDSI CCRYGG 1 cut(s) 1077
BstENI CCTNNNNNAGG 1 cut(s) 1059
BstF5I GGATG 3 cut(s) 141, 789, 1051
BstFNI CGCG 1 cut(s) 1107
BstH2I RGCGCY 3 cut(s) 90, 210, 1411
BstHHI GCGC 4 cut(s) 89, 209, 672, 1410
BstMAI GTCTC 2 cut(s) 273, 771
BstMCI CGRYCG 1 cut(s) 161
BstMWI GCNNNNNNNGC 9 cut(s) 84, 215, 224, 230, 319, 350, 654, 1296, 1414
BstNI CCWGG 3 cut(s) 324, 843, 1014
BstNSI RCATGY 4 cut(s) 269, 752, 767, 1284
BstSCI CCNGG 3 cut(s) 322, 841, 1012
BstSFI CTRYAG 2 cut(s) 73, 342
BstUI CGCG 1 cut(s) 1107
BstV1I GCAGC 6 cut(s) 87, 236, 328, 331, 641, 979
BstV2I GAAGAC 1 cut(s) 899
BstX2I RGATCY 1 cut(s) 968
BstYI RGATCY 1 cut(s) 968
BstZI CGGCCG 1 cut(s) 158
Bsu36I CCTNAGG 1 cut(s) 909
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 2 cut(s) 160, 1344
BtgI CCRYGG 1 cut(s) 1077
BtgZI GCGATG 2 cut(s) 663, 1230
BtsCI GGATG 3 cut(s) 141, 789, 1051
BtsIMutI CAGTG 2 cut(s) 688, 813
BveI ACCTGC 2 cut(s) 1153, 1208
Cac8I GCNNGC 1 cut(s) 1542
CciI TCATGA 1 cut(s) 63
CfoI GCGC 4 cut(s) 89, 209, 672, 1410
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 1322
Csp6I GTAC 7 cut(s) 16, 186, 263, 839, 854, 928, 1228
CviAII CATG 8 cut(s) 64, 266, 749, 758, 764, 973, 988, 1281
CviQI GTAC 7 cut(s) 16, 186, 263, 839, 854, 928, 1228
DdeI CTNAG 5 cut(s) 372, 680, 816, 909, 1115
DinI GGCGCC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 158
EagI CGGCCG 1 cut(s) 158
EclXI CGGCCG 1 cut(s) 158
Eco130I CCWWGG 3 cut(s) 246, 432, 1300
Eco31I GGTCTC 1 cut(s) 771
Eco47I GGWCC 1 cut(s) 1322
Eco52I CGGCCG 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 843
Eco81I CCTNAGG 1 cut(s) 909
EcoNI CCTNNNNNAGG 1 cut(s) 1059
EcoRII CCWGG 3 cut(s) 322, 841, 1012
EcoT14I CCWWGG 3 cut(s) 246, 432, 1300
EgeI GGCGCC 1 cut(s) 208
EheI GGCGCC 1 cut(s) 208
ErhI CCWWGG 3 cut(s) 246, 432, 1300
FaeI CATG 8 cut(s) 67, 269, 752, 761, 767, 976, 991, 1284
FalI AAGNNNNNCTT 2 cut(s) 691, 723
FaqI GGGAC 2 cut(s) 687, 859
FatI CATG 8 cut(s) 63, 265, 748, 757, 763, 972, 987, 1280
FauNDI CATATG 2 cut(s) 625, 1318
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 7 cut(s) 76, 140, 225, 320, 342, 655, 993
FokI GGATG 3 cut(s) 148, 776, 1038
Fsp4HI GCNGC 7 cut(s) 76, 140, 225, 320, 342, 655, 993
FspBI CTAG 1 cut(s) 867
FspI TGCGCA 1 cut(s) 671
GlaI GCGC 4 cut(s) 88, 208, 671, 1409
GluI GCNGC 7 cut(s) 76, 140, 225, 320, 342, 655, 993
GsaI CCCAGC 1 cut(s) 462
GsuI CTGGAG 1 cut(s) 627
HaeII RGCGCY 3 cut(s) 90, 210, 1411
HaeIII GGCC 2 cut(s) 160, 1344
HapII CCGG 3 cut(s) 161, 210, 219
HhaI GCGC 4 cut(s) 89, 209, 672, 1410
Hin1I GRCGYC 2 cut(s) 192, 207
Hin1II CATG 8 cut(s) 67, 269, 752, 761, 767, 976, 991, 1284
Hin6I GCGC 4 cut(s) 87, 207, 670, 1408
HinP1I GCGC 4 cut(s) 87, 207, 670, 1408
HinfI GANTC 6 cut(s) 30, 359, 820, 1034, 1166, 1308
HpaII CCGG 3 cut(s) 161, 210, 219
HphI GGTGA 7 cut(s) 320, 559, 598, 777, 884, 1422, 1548
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 3 cut(s) 375, 451, 1090
Hpy188III TCNNGA 1 cut(s) 64
Hpy8I GTNNAC 1 cut(s) 263
Hpy99I CGWCG 2 cut(s) 194, 197
HpyAV CCTTC 6 cut(s) 245, 370, 792, 867, 1437, 1499
HpyCH4III ACNGT 4 cut(s) 149, 737, 1078, 1537
HpyCH4IV ACGT 3 cut(s) 18, 192, 1126
HpyF10VI GCNNNNNNNGC 9 cut(s) 84, 215, 224, 230, 319, 350, 654, 1296, 1414
HpyF3I CTNAG 5 cut(s) 372, 680, 816, 909, 1115
HpySE526I ACGT 3 cut(s) 18, 192, 1126
Hsp92I GRCGYC 2 cut(s) 192, 207
Hsp92II CATG 8 cut(s) 67, 269, 752, 761, 767, 976, 991, 1284
HspAI GCGC 4 cut(s) 87, 207, 670, 1408
KasI GGCGCC 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 66
LmnI GCTCC 3 cut(s) 436, 634, 910
Lsp1109I GCAGC 6 cut(s) 87, 236, 328, 331, 641, 979
LweI GCATC 8 cut(s) 126, 358, 1060, 1129, 1253, 1449, 1481, 1488
MaeI CTAG 1 cut(s) 867
MaeII ACGT 3 cut(s) 18, 192, 1126
MaeIII GTNAC 1 cut(s) 547
MboII GAAGA 4 cut(s) 734, 904, 1091, 1103
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 1 cut(s) 968
MluCI AATT 5 cut(s) 300, 363, 530, 1020, 1156
Mly113I GGCGCC 1 cut(s) 207
MlyI GAGTC 2 cut(s) 39, 368
MroXI GAANNNNTTC 1 cut(s) 1312
MseI TTAA 4 cut(s) 1058, 1347, 1412, 1466
MslI CAYNNNNRTG 1 cut(s) 762
MspA1I CMGCKG 1 cut(s) 925
MspCI CTTAAG 1 cut(s) 1465
MspI CCGG 3 cut(s) 161, 210, 219
MspR9I CCNGG 3 cut(s) 324, 843, 1014
MunI CAATTG 1 cut(s) 363
Mva1269I GAATGC 1 cut(s) 501
MvaI CCWGG 3 cut(s) 324, 843, 1014
MvnI CGCG 1 cut(s) 1107
MwoI GCNNNNNNNGC 9 cut(s) 84, 215, 224, 230, 319, 350, 654, 1296, 1414
NarI GGCGCC 1 cut(s) 207
NdeI CATATG 2 cut(s) 625, 1318
NlaIII CATG 8 cut(s) 67, 269, 752, 761, 767, 976, 991, 1284
NlaIV GGNNCC 4 cut(s) 208, 906, 915, 1539
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 1 cut(s) 547
NsbI TGCGCA 1 cut(s) 671
NspI RCATGY 4 cut(s) 269, 752, 767, 1284
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 2 cut(s) 1153, 1208
PciI ACATGT 2 cut(s) 265, 763
PctI GAATGC 1 cut(s) 501
PdmI GAANNNNTTC 1 cut(s) 1312
PfeI GAWTC 4 cut(s) 820, 1034, 1166, 1308
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 7 cut(s) 77, 141, 226, 321, 343, 656, 994
PleI GAGTC 2 cut(s) 38, 367
PluTI GGCGCC 1 cut(s) 210
PpsI GAGTC 2 cut(s) 38, 367
Ppu21I YACGTR 1 cut(s) 1127
PscI ACATGT 2 cut(s) 265, 763
PsiI TTATAA 1 cut(s) 1496
Psp6I CCWGG 3 cut(s) 322, 841, 1012
PspFI CCCAGC 1 cut(s) 458
PspGI CCWGG 3 cut(s) 322, 841, 1012
PspN4I GGNNCC 4 cut(s) 208, 906, 915, 1539
PspPI GGNCC 1 cut(s) 1322
PstI CTGCAG 2 cut(s) 77, 346
PsuI RGATCY 1 cut(s) 968
PsyI GACNNNGTC 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 925
RsaI GTAC 7 cut(s) 17, 187, 264, 840, 855, 929, 1229
RsaNI GTAC 7 cut(s) 16, 186, 263, 839, 854, 928, 1228
RseI CAYNNNNRTG 1 cut(s) 762
SaqAI TTAA 4 cut(s) 1058, 1347, 1412, 1466
SatI GCNGC 7 cut(s) 76, 140, 225, 320, 342, 655, 993
Sau96I GGNCC 1 cut(s) 1322
SchI GAGTC 2 cut(s) 39, 368
ScrFI CCNGG 3 cut(s) 324, 843, 1014
SfaNI GCATC 8 cut(s) 126, 358, 1060, 1129, 1253, 1449, 1481, 1488
SfcI CTRYAG 2 cut(s) 73, 342
SfoI GGCGCC 1 cut(s) 208
SinI GGWCC 1 cut(s) 1322
SmiMI CAYNNNNRTG 1 cut(s) 762
SmlI CTYRAG 2 cut(s) 283, 1465
SmoI CTYRAG 2 cut(s) 283, 1465
Sse9I AATT 5 cut(s) 300, 363, 530, 1020, 1156
SsiI CCGC 3 cut(s) 140, 1105, 1358
SspDI GGCGCC 1 cut(s) 206
SspMI CTAG 1 cut(s) 867
StyD4I CCNGG 3 cut(s) 322, 841, 1012
StyI CCWWGG 3 cut(s) 246, 432, 1300
TaaI ACNGT 4 cut(s) 149, 737, 1078, 1537
TaiI ACGT 3 cut(s) 21, 195, 1129
TaqI TCGA 2 cut(s) 561, 834
TasI AATT 5 cut(s) 300, 363, 530, 1020, 1156
TatI WGTACW 3 cut(s) 262, 927, 1227
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 4 cut(s) 820, 1034, 1166, 1308
Tru1I TTAA 4 cut(s) 1058, 1347, 1412, 1466
Tru9I TTAA 4 cut(s) 1058, 1347, 1412, 1466
TscAI CASTG 2 cut(s) 688, 820
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 6 cut(s) 75, 224, 319, 341, 654, 992
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 1026, 1305, 1556
TspRI CASTG 2 cut(s) 688, 820
Tth111I GACNNNGTC 1 cut(s) 194
Vha464I CTTAAG 1 cut(s) 1465
VpaK11BI GGWCC 1 cut(s) 1322
XagI CCTNNNNNAGG 1 cut(s) 1059
XceI RCATGY 4 cut(s) 269, 752, 767, 1284
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XmnI GAANNNNTTC 1 cut(s) 1312
XspI CTAG 1 cut(s) 867
ZraI GACGTC 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.