FvH4_2g02600

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000108
Physical Location & Seq
Forward (+)
2073280 .. 2075734
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02600.t1

Sequence Viewer

Length: 1587 bp
ATGGTTTCATACGAGTATGTTCTGGGTGGGGTGTTAGTTTCTTTGCTAGGCTCTATTTTCTTCATGATCATCAGCTCTACTTTGGGAGGAAAGAAACAATTCAAGGCTTCAGGTGTAGAAAAGAGTAATGGGTACGGAAACGGTGAGTTTCTGCCGGAAATGGAGGAGGAGAAGAGTACTGATGTCGTCATTGTTGGCGCCGGAGTTGCCGGTGCAGCTCTTGCTTACACTCTTGCCAAGGAAGGACGCCGCGTACATGTAATTGAAAGAGACTTGAGCGAGCCAGACAGAATTGTTGGGGAGCTGTTGCAGCCTGGGGGATATCTCAAATTGATTGAGTTGGGTCTTGAGGATTGTGCAAATGAGTCAATAGATGCCCAGAAGGTGTTTGGTTATGCTCTTTACAAAGATGGCAAGGATACAACACTGTCTTATCCTTTGGAGAAATACAGTTCAGATGTGGCTGGTAGGAGTTTCCACAATGGGCGTTTCATTCAAAGAATGCGTGAAAAAGCCATAACTCTTTCAAATGTTAAATTGGAACAAGGGTCAGTAACGACACTAATTGAAGAAAAGGGCATTGTCAAAGGGGTGATTTACAAGAACAAGGCTGGAGAGGAGATGAGAACATATGCTCCCCTCACAATCGTCTGTGATGGTTGCTTTTCAAATCTGCGAAAATCTCTCAGTGCTCCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGATCTTGGAGAACTGTGAGCTTCCGCACGCAAATCACGGACATGTCATTTTGGGAGACCCTTCACCCATCCTATTTTATCCCATCAGTTCCACCGAGGTTCGCTGTTTGGTGGATGTGCCTGGAACAAAAGTACCTTCAGTTGCTAATGGTGAAATGGCTAACTATTTGAAAACTGTTGTTGCTCCTCAGGTTCCCCCACAGCTGCACAATGCTTTTATGGTTGCAGTAGAGAAAGGAAACATTAGATCGATGCAAAACAGAAGCATGGCTGCTAATCCGGTTCCAACTCCTGGTGCACTTTTATTAGGCGACTCTTTCAACATGAGACATCCTTTAACAGGAGGAGGAATGACCGTGGCTCTTTCGGACATTGTTCTCCTCCGTGATCTTCTTAGTCCCCTACATGATCTCAATGATGCACCTGCCTTGTGCAATTACCTTGAATCATTCTACACACTTCGTAAGCCTGTGTCTTCTACCATAAACACATTGGCAGGTGCTTTGTACAAGGTGTTCTGTGCCTCACCTGATCCGGCAAGACAGGAAATGCGTGAAGCATGTTTCGGCTATTTGAGCCTTGGAGGTATCTGTTCTTATGGACCGGTATCTCTTCTCTCTGGTCTTAACCCTCGTCCGCCGCACTTGTTTCTCCATTTCTTTGCTGTTGCTATATATGGCGTAGGGCGCTTGATGTTTCCATTCCCTTCACCCAAACGCATATGGCTTGGGGCTAGATTGATCTTGAGTGCATCAGGCATCATATTCCCAATTATAAAGGGTGAAGGAGTCAGACAGATGTTCTTTCCTGCAACTGTACCAGCATATTACAGATCTCCTCCTGTTTACTGCAGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

529

Amino Acids

57.38

Weight (kDa)

8.17

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 60 - 374 1.9e-18 FAD binding domain
DAO PF01266 61 - 91 4.9e-08 FAD dependent oxidoreductase
FAD_binding_2 PF00890 61 - 90 1e-05 FAD binding domain
NAD_binding_8 PF13450 64 - 90 2.1e-06 NAD(P)-binding Rossmann-like domain
SE PF08491 212 - 484 8.3e-117 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1502
AarI CACCTGC 2 cut(s) 1159, 1214
Acc36I ACCTGC 2 cut(s) 1159, 1214
AccB1I GGYRCC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 1019
AccII CGCG 1 cut(s) 252
AciI CCGC 4 cut(s) 250, 752, 1364, 1367
AclWI GGATC 1 cut(s) 1253
AcuI CTGAAG 2 cut(s) 93, 849
AcyI GRCGYC 2 cut(s) 198, 247
AfaI GTAC 6 cut(s) 134, 178, 255, 861, 1235, 1545
AfiI CCNNNNNNNGG 2 cut(s) 1019, 1067
AflIII ACRYGT 2 cut(s) 256, 769
AgeI ACCGGT 1 cut(s) 1330
AjnI CCWGG 3 cut(s) 313, 847, 1018
AloI GAACNNNNNNTCC 2 cut(s) 619, 651
AluBI AGCT 5 cut(s) 75, 218, 304, 748, 931
AluI AGCT 5 cut(s) 75, 218, 304, 748, 931
Alw21I GWGCWC 2 cut(s) 694, 1027
Alw26I GTCTC 3 cut(s) 264, 777, 1048
Alw44I GTGCAC 1 cut(s) 1023
AlwI GGATC 1 cut(s) 1253
AlwNI CAGNNNCTG 1 cut(s) 1584
ApaLI GTGCAC 1 cut(s) 1023
ApeKI GCWGC 4 cut(s) 215, 310, 931, 998
AsiGI ACCGGT 1 cut(s) 1330
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 2 cut(s) 200, 1416
AspS9I GGNCC 1 cut(s) 1328
AsuHPI GGTGA 7 cut(s) 155, 604, 783, 890, 1245, 1428, 1520
AvaII GGWCC 1 cut(s) 1328
AxyI CCTNAGG 1 cut(s) 915
BaeGI GKGCMC 1 cut(s) 1027
BaeI ACNNNNGTAYC 2 cut(s) 843, 876
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 1194
Bbv12I GWGCWC 2 cut(s) 694, 1027
BbvI GCAGC 4 cut(s) 227, 322, 918, 985
BccI CCATC 4 cut(s) 404, 650, 803, 818
BciT130I CCWGG 3 cut(s) 315, 849, 1020
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 3 cut(s) 264, 777, 1048
BfaI CTAG 2 cut(s) 47, 1461
BfmI CTRYAG 1 cut(s) 1576
BfoI RGCGCY 2 cut(s) 201, 1417
BfuAI ACCTGC 2 cut(s) 1159, 1214
BfuI GTATCC 1 cut(s) 412
BglII AGATCT 1 cut(s) 1559
BisI GCNGC 6 cut(s) 216, 250, 311, 932, 999, 1367
BlsI GCNGC 6 cut(s) 217, 251, 312, 933, 1000, 1368
BmcAI AGTACT 1 cut(s) 178
Bme1390I CCNGG 3 cut(s) 315, 849, 1020
Bme18I GGWCC 1 cut(s) 1328
BmgT120I GGNCC 1 cut(s) 1328
BmiI GGNNCC 3 cut(s) 199, 921, 1011
BmrFI CCNGG 3 cut(s) 315, 849, 1020
BmsI GCATC 5 cut(s) 364, 969, 1135, 1487, 1494
BpiI GAAGAC 1 cut(s) 1194
BpmI CTGGAG 1 cut(s) 633
BpuEI CTTGAG 3 cut(s) 295, 368, 1492
Bsa29I ATCGAT 1 cut(s) 977
BsaHI GRCGYC 2 cut(s) 198, 247
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 5 cut(s) 237, 314, 822, 1083, 1306
BsaWI WCCGGW 2 cut(s) 1006, 1330
BsaXI ACNNNNNCTCC 2 cut(s) 619, 649
Bsc4I CCNNNNNNNGG 2 cut(s) 1019, 1067
Bse118I RCCGGY 2 cut(s) 209, 1330
Bse21I CCTNAGG 1 cut(s) 915
BseBI CCWGG 3 cut(s) 315, 849, 1020
BseCI ATCGAT 1 cut(s) 977
BseDI CCNNGG 5 cut(s) 237, 314, 822, 1083, 1306
BseGI GGATG 3 cut(s) 795, 847, 1057
BseLI CCNNNNNNNGG 2 cut(s) 1019, 1067
BseMII CTCAG 2 cut(s) 700, 929
BseRI GAGGAG 7 cut(s) 179, 182, 632, 903, 1086, 1097, 1554
BseSI GKGCMC 1 cut(s) 1027
BseXI GCAGC 4 cut(s) 227, 322, 918, 985
BsgI GTGCAG 2 cut(s) 234, 917
Bsh1236I CGCG 1 cut(s) 252
BshNI GGYRCC 1 cut(s) 197
BshTI ACCGGT 1 cut(s) 1330
BshVI ATCGAT 1 cut(s) 977
BsiHKAI GWGCWC 2 cut(s) 694, 1027
BsiSI CCGG 6 cut(s) 155, 201, 210, 1007, 1262, 1331
BslFI GGGAC 2 cut(s) 693, 1110
BslI CCNNNNNNNGG 2 cut(s) 1019, 1067
BsmAI GTCTC 3 cut(s) 264, 777, 1048
BsmFI GGGAC 2 cut(s) 693, 1110
BsmI GAATGC 1 cut(s) 507
Bso31I GGTCTC 1 cut(s) 777
Bsp1286I GDGCHC 2 cut(s) 694, 1027
Bsp1407I TGTACA 1 cut(s) 1233
Bsp143I GATC 8 cut(s) 66, 729, 974, 1114, 1135, 1258, 1467, 1559
BspACI CCGC 4 cut(s) 250, 752, 1364, 1367
BspCNI CTCAG 2 cut(s) 699, 928
BspDI ATCGAT 1 cut(s) 977
BspFNI CGCG 1 cut(s) 252
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 3 cut(s) 199, 921, 1011
BspMAI CTGCAG 1 cut(s) 1580
BspMI ACCTGC 2 cut(s) 1159, 1214
BspPI GGATC 1 cut(s) 1253
BspT107I GGYRCC 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 777
BsrFI RCCGGY 2 cut(s) 209, 1330
BsrGI TGTACA 1 cut(s) 1233
BssAI RCCGGY 2 cut(s) 209, 1330
BssECI CCNNGG 5 cut(s) 237, 314, 822, 1083, 1306
BssMI GATC 8 cut(s) 66, 729, 974, 1114, 1135, 1258, 1467, 1559
BssNI GRCGYC 2 cut(s) 198, 247
BssT1I CCWWGG 2 cut(s) 237, 1306
Bst2UI CCWGG 3 cut(s) 315, 849, 1020
Bst4CI ACNGT 7 cut(s) 143, 429, 452, 743, 904, 1084, 1543
Bst6I CTCTTC 2 cut(s) 167, 1344
BstACI GRCGYC 2 cut(s) 198, 247
BstAPI GCANNNNNTGC 1 cut(s) 221
BstAUI TGTACA 1 cut(s) 1233
BstC8I GCNNGC 2 cut(s) 281, 756
BstDEI CTNAG 3 cut(s) 686, 915, 1121
BstDSI CCRYGG 1 cut(s) 1083
BstENI CCTNNNNNAGG 1 cut(s) 1065
BstF5I GGATG 3 cut(s) 795, 847, 1057
BstFNI CGCG 1 cut(s) 252
BstH2I RGCGCY 2 cut(s) 201, 1417
BstHHI GCGC 2 cut(s) 200, 1416
BstKTI GATC 8 cut(s) 69, 732, 977, 1117, 1138, 1261, 1470, 1562
BstMAI GTCTC 3 cut(s) 264, 777, 1048
BstMBI GATC 8 cut(s) 66, 729, 974, 1114, 1135, 1258, 1467, 1559
BstMWI GCNNNNNNNGC 6 cut(s) 206, 215, 221, 310, 1302, 1413
BstNI CCWGG 3 cut(s) 315, 849, 1020
BstNSI RCATGY 3 cut(s) 260, 773, 1290
BstSCI CCNGG 3 cut(s) 313, 847, 1018
BstSFI CTRYAG 1 cut(s) 1576
BstSLI GKGCMC 1 cut(s) 1027
BstUI CGCG 1 cut(s) 252
BstV1I GCAGC 4 cut(s) 227, 322, 918, 985
BstV2I GAAGAC 1 cut(s) 1194
BstX2I RGATCY 1 cut(s) 1559
BstYI RGATCY 1 cut(s) 1559
Bsu15I ATCGAT 1 cut(s) 977
Bsu36I CCTNAGG 1 cut(s) 915
BsuI GTATCC 1 cut(s) 412
BsuTUI ATCGAT 1 cut(s) 977
BtgI CCRYGG 1 cut(s) 1083
BtsCI GGATG 3 cut(s) 795, 847, 1057
BtsIMutI CAGTG 2 cut(s) 425, 694
BveI ACCTGC 2 cut(s) 1159, 1214
Cac8I GCNNGC 2 cut(s) 281, 756
CaiI CAGNNNCTG 1 cut(s) 1584
CciI TCATGA 1 cut(s) 63
CfoI GCGC 2 cut(s) 200, 1416
Cfr10I RCCGGY 2 cut(s) 209, 1330
Cfr13I GGNCC 1 cut(s) 1328
ClaI ATCGAT 1 cut(s) 977
CseI GACGC 1 cut(s) 255
Csp6I GTAC 6 cut(s) 133, 177, 254, 860, 1234, 1544
CspAI ACCGGT 1 cut(s) 1330
CviAII CATG 7 cut(s) 64, 257, 770, 994, 1051, 1133, 1287
CviQI GTAC 6 cut(s) 133, 177, 254, 860, 1234, 1544
DdeI CTNAG 3 cut(s) 686, 915, 1121
DinI GGCGCC 1 cut(s) 199
DpnI GATC 8 cut(s) 68, 731, 976, 1116, 1137, 1260, 1469, 1561
DpnII GATC 8 cut(s) 66, 729, 974, 1114, 1135, 1258, 1467, 1559
Eam1104I CTCTTC 2 cut(s) 167, 1344
EarI CTCTTC 2 cut(s) 167, 1344
EciI GGCGGA 1 cut(s) 1353
Eco130I CCWWGG 2 cut(s) 237, 1306
Eco31I GGTCTC 1 cut(s) 777
Eco32I GATATC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 1328
Eco57I CTGAAG 2 cut(s) 93, 849
Eco81I CCTNAGG 1 cut(s) 915
EcoNI CCTNNNNNAGG 1 cut(s) 1065
EcoRII CCWGG 3 cut(s) 313, 847, 1018
EcoRV GATATC 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 237, 1306
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 2 cut(s) 237, 1306
FaeI CATG 7 cut(s) 67, 260, 773, 997, 1054, 1136, 1290
FalI AAGNNNNNCTT 2 cut(s) 697, 729
FaqI GGGAC 2 cut(s) 693, 1110
FatI CATG 7 cut(s) 63, 256, 769, 993, 1050, 1132, 1286
FauNDI CATATG 2 cut(s) 631, 1448
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 6 cut(s) 216, 250, 311, 932, 999, 1367
FokI GGATG 3 cut(s) 782, 854, 1044
Fsp4HI GCNGC 6 cut(s) 216, 250, 311, 932, 999, 1367
FspBI CTAG 2 cut(s) 47, 1461
GlaI GCGC 2 cut(s) 199, 1415
GluI GCNGC 6 cut(s) 216, 250, 311, 932, 999, 1367
GsuI CTGGAG 1 cut(s) 633
HaeII RGCGCY 2 cut(s) 201, 1417
HapII CCGG 6 cut(s) 155, 201, 210, 1007, 1262, 1331
HgaI GACGC 1 cut(s) 255
HhaI GCGC 2 cut(s) 200, 1416
Hin1I GRCGYC 2 cut(s) 198, 247
Hin1II CATG 7 cut(s) 67, 260, 773, 997, 1054, 1136, 1290
Hin6I GCGC 2 cut(s) 198, 1414
HinP1I GCGC 2 cut(s) 198, 1414
HinfI GANTC 4 cut(s) 365, 1040, 1172, 1515
HpaII CCGG 6 cut(s) 155, 201, 210, 1007, 1262, 1331
HphI GGTGA 7 cut(s) 155, 604, 783, 890, 1245, 1428, 1520
Hpy166II GTNNAC 2 cut(s) 1025, 1573
Hpy188I TCNGA 3 cut(s) 457, 1096, 1520
Hpy188III TCNNGA 3 cut(s) 64, 347, 1471
Hpy8I GTNNAC 2 cut(s) 1025, 1573
HpyAV CCTTC 6 cut(s) 236, 376, 798, 873, 1443, 1505
HpyCH4III ACNGT 7 cut(s) 143, 429, 452, 743, 904, 1084, 1543
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 215, 221, 310, 1302, 1413
HpyF3I CTNAG 3 cut(s) 686, 915, 1121
Hsp92I GRCGYC 2 cut(s) 198, 247
Hsp92II CATG 7 cut(s) 67, 260, 773, 997, 1054, 1136, 1290
HspAI GCGC 2 cut(s) 198, 1414
KasI GGCGCC 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 8 cut(s) 66, 729, 974, 1114, 1135, 1258, 1467, 1559
LmnI GCTCC 4 cut(s) 301, 640, 697, 916
Lsp1109I GCAGC 4 cut(s) 227, 322, 918, 985
LweI GCATC 5 cut(s) 364, 969, 1135, 1487, 1494
MaeI CTAG 2 cut(s) 47, 1461
MaeIII GTNAC 1 cut(s) 553
MalI GATC 8 cut(s) 68, 731, 976, 1116, 1137, 1260, 1469, 1561
MboI GATC 8 cut(s) 66, 729, 974, 1114, 1135, 1258, 1467, 1559
MboII GAAGA 6 cut(s) 52, 184, 581, 1109, 1194, 1331
MflI RGATCY 1 cut(s) 1559
MhlI GDGCHC 2 cut(s) 694, 1027
MluCI AATT 8 cut(s) 98, 261, 291, 329, 536, 564, 1162, 1497
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 3 cut(s) 374, 1034, 1524
MmeI TCCRAC 1 cut(s) 1037
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 3 cut(s) 534, 1064, 1353
MslI CAYNNNNRTG 1 cut(s) 768
MspA1I CMGCKG 1 cut(s) 931
MspI CCGG 6 cut(s) 155, 201, 210, 1007, 1262, 1331
MspR9I CCNGG 3 cut(s) 315, 849, 1020
Mva1269I GAATGC 1 cut(s) 507
MvaI CCWGG 3 cut(s) 315, 849, 1020
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 6 cut(s) 206, 215, 221, 310, 1302, 1413
NarI GGCGCC 1 cut(s) 198
NdeI CATATG 2 cut(s) 631, 1448
NdeII GATC 8 cut(s) 66, 729, 974, 1114, 1135, 1258, 1467, 1559
NlaIII CATG 7 cut(s) 67, 260, 773, 997, 1054, 1136, 1290
NlaIV GGNNCC 3 cut(s) 199, 921, 1011
NspI RCATGY 3 cut(s) 260, 773, 1290
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 2 cut(s) 1159, 1214
PciI ACATGT 2 cut(s) 256, 769
PctI GAATGC 1 cut(s) 507
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 1 cut(s) 1172
PflMI CCANNNNNTGG 1 cut(s) 1019
PinAI ACCGGT 1 cut(s) 1330
PkrI GCNGC 6 cut(s) 217, 251, 312, 933, 1000, 1368
PleI GAGTC 3 cut(s) 373, 1034, 1523
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 3 cut(s) 373, 1034, 1523
PscI ACATGT 2 cut(s) 256, 769
PsiI TTATAA 1 cut(s) 1502
Psp6I CCWGG 3 cut(s) 313, 847, 1018
PspGI CCWGG 3 cut(s) 313, 847, 1018
PspN4I GGNNCC 3 cut(s) 199, 921, 1011
PspPI GGNCC 1 cut(s) 1328
PsrI GAACNNNNNNTAC 2 cut(s) 1226, 1258
PstI CTGCAG 1 cut(s) 1580
PstNI CAGNNNCTG 1 cut(s) 1584
PsuI RGATCY 1 cut(s) 1559
PvuII CAGCTG 1 cut(s) 931
RsaI GTAC 6 cut(s) 134, 178, 255, 861, 1235, 1545
RsaNI GTAC 6 cut(s) 133, 177, 254, 860, 1234, 1544
RseI CAYNNNNRTG 1 cut(s) 768
SaqAI TTAA 3 cut(s) 534, 1064, 1353
SatI GCNGC 6 cut(s) 216, 250, 311, 932, 999, 1367
Sau3AI GATC 8 cut(s) 66, 729, 974, 1114, 1135, 1258, 1467, 1559
Sau96I GGNCC 1 cut(s) 1328
ScaI AGTACT 1 cut(s) 178
SchI GAGTC 3 cut(s) 374, 1034, 1524
ScrFI CCNGG 3 cut(s) 315, 849, 1020
SduI GDGCHC 2 cut(s) 694, 1027
SfaNI GCATC 5 cut(s) 364, 969, 1135, 1487, 1494
SfcI CTRYAG 1 cut(s) 1576
SfoI GGCGCC 1 cut(s) 199
SinI GGWCC 1 cut(s) 1328
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 3 cut(s) 274, 347, 1471
SmoI CTYRAG 3 cut(s) 274, 347, 1471
Sse9I AATT 8 cut(s) 98, 261, 291, 329, 536, 564, 1162, 1497
SsiI CCGC 4 cut(s) 250, 752, 1364, 1367
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 2 cut(s) 47, 1461
StyD4I CCNGG 3 cut(s) 313, 847, 1018
StyI CCWWGG 2 cut(s) 237, 1306
TaaI ACNGT 7 cut(s) 143, 429, 452, 743, 904, 1084, 1543
TaqI TCGA 1 cut(s) 977
TasI AATT 8 cut(s) 98, 261, 291, 329, 536, 564, 1162, 1497
TatI WGTACW 2 cut(s) 176, 1233
TauI GCSGC 2 cut(s) 252, 1369
TfiI GAWTC 1 cut(s) 1172
Tru1I TTAA 3 cut(s) 534, 1064, 1353
Tru9I TTAA 3 cut(s) 534, 1064, 1353
TscAI CASTG 2 cut(s) 432, 694
TseI GCWGC 4 cut(s) 215, 310, 931, 998
TspDTI ATGAA 2 cut(s) 52, 481
TspGWI ACGGA 3 cut(s) 150, 780, 1100
TspRI CASTG 2 cut(s) 432, 694
Van91I CCANNNNNTGG 1 cut(s) 1019
VneI GTGCAC 1 cut(s) 1023
VpaK11BI GGWCC 1 cut(s) 1328
XagI CCTNNNNNAGG 1 cut(s) 1065
XceI RCATGY 3 cut(s) 260, 773, 1290
XcmI CCANNNNNNNNNTGG 2 cut(s) 386, 1216
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 47, 1461
ZrmI AGTACT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.