Rh4AG220000

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000108
Physical Location & Seq
Reverse (-)
51790541 .. 51793180
2640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG220000.1

Sequence Viewer

Length: 1488 bp
ATGGTGTCTTACGAGTATATTTTGGGTGGGGTCTTGGTTTCATTGCTGGGCTCTGTTTTCTTCATGATCATCAGCACTACTTTGGGTGGAAAGAAACCATGCAAGGCTTCAAATGGAGGTGTAAAAAGTAACGATGGGTTTGGGAACAGTGCTATTCAGCCGGAAATGGAGATGAAAAGTACCGATGTCGTCATTGTCGGTGCCGGAGTTGCCGGTGCGGCTCTTGCTTACACTCTTGCCAAGGTCAATTGTGCAAATGAGTCAATTGATGCTCAGAAGGTGTTTGGTTATGCTCTTTACAAAGATGGCAAGGATACCAAACTGACTTATCCCTTAGAGAAATACAGTACAGATGTGGCTGGGAGAAGTTTCCACAATGGGCGTTTCATCCAAAGGATGCGCGAAAAAGCTATGACTCTTTCAAATGTAAAATTGGAACAAGGATCAGTGACAACATTAATCGAGGAAAAGGGCATTGTCAAAGGGGTGATTTACAAGAACAAGGCTGGAGAGGAGATGAGAACATATGCTCCACTAACAATCGTGTGTGATGGTTGCTTTTCAAATCTGCGCAAGTCTCTCAGTGCACCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGATCTTGGAGAACTGTGACCTTCCACACGCAAATCATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTCTTCTATCCTATCAGTAGCACCGAGGTTCGTTGTTTGGTGGATGTACCTGGAACAAAAGTACCTTCAGTAGCTAGTGGTGAAATGGCTAACTATTTGAAAACCGTGGTGGCTCCTCAGATTCCCCCACAGCTGCACAATGCTTTTATGGCCGCAGTAGAGAAGGGAAACATCAGAACAATGCAAAACAAAAGCATGGCTGCTAATCCTGTCCCTACTCCTGGTGCAATTTTATTAGGGGATGCATTCAACATGAGGCACCCTTTAACAGGAGGAGGAATGACCGTTGCTCTTTCAGACATTGTTCTTCTCCGTGATCTTCTTAGACCCCTACATGATCTCAATGATGCACCTGCTTTGTGCAATTACCTTGAATCATTCTACACACTGCGCAAGCCTGTATCATCTACCATAAACACATTGGCAGGTGCTTTGTACAAAGTGTTTTGTGCATCACCGGATCCAGCAAGACAGGAAATGCGTGAAGCATGTTTTGGCTATTTGAGCTTTGGAGGCATCTGTTCATACGGACCAGTATCTCTTCTCTCTGGTCTTAACCCTCAGCCACTGCACTTGTTTCTCCATTTCTTTGCTGTTGCCATCTATGGTGTCGGCCGCTTAATGCTTCCATTCCCTTCACCTAAACGCATGTGGCTTGGGATTCGATTGATCTTGAGTGCAGCTGGCATCATATTCCCCATTATAAAAGGTGAAGGAGTTAGACAGATGTTCTTTCCTGCCACAGTACCAGCATATTACAGATCTCCTCCTGTTCATCAACGAAATGGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

495

Amino Acids

53.47

Weight (kDa)

9.02

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 134 - 336 8.1e-09 FAD binding domain
SE PF08491 177 - 449 6.4e-118 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1397
AarI CACCTGC 2 cut(s) 1054, 1109
Acc16I TGCGCA 2 cut(s) 572, 1085
Acc36I ACCTGC 2 cut(s) 1054, 1109
AccB1I GGYRCC 2 cut(s) 200, 951
AccII CGCG 1 cut(s) 402
AciI CCGC 3 cut(s) 218, 846, 1309
AclWI GGATC 3 cut(s) 451, 1148, 1161
AcoI YGGCCR 2 cut(s) 843, 1306
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 6 cut(s) 181, 349, 741, 756, 1130, 1440
AfiI CCNNNNNNNGG 2 cut(s) 914, 962
AflIII ACRYGT 1 cut(s) 664
AgsI TTSAA 7 cut(s) 111, 423, 564, 599, 793, 943, 1067
AjnI CCWGG 2 cut(s) 742, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 1170, 1202
AloI GAACNNNNNNTCC 2 cut(s) 514, 546
AluBI AGCT 5 cut(s) 410, 767, 826, 1200, 1376
AluI AGCT 5 cut(s) 410, 767, 826, 1200, 1376
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 2 cut(s) 582, 672
Alw44I GTGCAC 1 cut(s) 585
AlwI GGATC 3 cut(s) 451, 1148, 1161
AlwNI CAGNNNCTG 1 cut(s) 1261
AoxI GGCC 2 cut(s) 843, 1306
ApaLI GTGCAC 1 cut(s) 585
ApeKI GCWGC 3 cut(s) 826, 893, 1373
AseI ATTAAT 1 cut(s) 458
AspLEI GCGC 3 cut(s) 402, 573, 1086
AspS9I GGNCC 1 cut(s) 1223
AsuHPI GGTGA 6 cut(s) 499, 678, 785, 1140, 1323, 1415
AvaII GGWCC 1 cut(s) 1223
BaeGI GKGCMC 1 cut(s) 589
BaeI ACNNNNGTAYC 2 cut(s) 738, 771
BamHI GGATCC 1 cut(s) 1153
BanI GGYRCC 2 cut(s) 200, 951
BanII GRGCYC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 589
BbvCI CCTCAGC 1 cut(s) 1254
BbvI GCAGC 3 cut(s) 813, 880, 1385
BccI CCATC 5 cut(s) 128, 299, 545, 698, 1301
BciT130I CCWGG 2 cut(s) 744, 915
BciVI GTATCC 1 cut(s) 307
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 582, 672
BfaI CTAG 2 cut(s) 768, 1486
BfuAI ACCTGC 2 cut(s) 1054, 1109
BfuI GTATCC 1 cut(s) 307
BglI GCCNNNNNGGC 1 cut(s) 218
BglII AGATCT 1 cut(s) 1454
BisI GCNGC 6 cut(s) 219, 827, 846, 894, 1309, 1374
BlsI GCNGC 6 cut(s) 220, 828, 847, 895, 1310, 1375
Bme1390I CCNGG 2 cut(s) 744, 915
Bme18I GGWCC 1 cut(s) 1223
BmgT120I GGNCC 1 cut(s) 1223
BmiI GGNNCC 4 cut(s) 202, 807, 953, 1155
BmrFI CCNGG 2 cut(s) 744, 915
BmsI GCATC 7 cut(s) 259, 387, 925, 1030, 1154, 1218, 1389
BpmI CTGGAG 1 cut(s) 528
Bpu10I CCTNAGC 1 cut(s) 1254
BpuEI CTTGAG 1 cut(s) 1387
BsaI GGTCTC 1 cut(s) 672
BsaJI CCNNGG 3 cut(s) 240, 717, 798
BsaWI WCCGGW 1 cut(s) 1150
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 2 cut(s) 914, 962
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 1226
Bse3DI GCAATG 1 cut(s) 41
BseBI CCWGG 2 cut(s) 744, 915
BseDI CCNNGG 3 cut(s) 240, 717, 798
BseGI GGATG 5 cut(s) 387, 402, 690, 742, 940
BseLI CCNNNNNNNGG 2 cut(s) 914, 962
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 4 cut(s) 287, 595, 824, 1268
BseNI ACTGG 1 cut(s) 1226
BseRI GAGGAG 4 cut(s) 527, 798, 981, 1449
BseSI GKGCMC 1 cut(s) 589
BseX3I CGGCCG 1 cut(s) 1306
BseXI GCAGC 3 cut(s) 813, 880, 1385
BseYI CCCAGC 2 cut(s) 46, 359
BsgI GTGCAG 3 cut(s) 812, 1247, 1392
Bsh1236I CGCG 1 cut(s) 402
Bsh1285I CGRYCG 1 cut(s) 1309
BshFI GGCC 2 cut(s) 845, 1308
BshNI GGYRCC 2 cut(s) 200, 951
BsiEI CGRYCG 1 cut(s) 1309
BsiHKAI GWGCWC 1 cut(s) 589
BsiSI CCGG 4 cut(s) 161, 204, 213, 1151
BslFI GGGAC 2 cut(s) 588, 890
BslI CCNNNNNNNGG 2 cut(s) 914, 962
BsmAI GTCTC 2 cut(s) 582, 672
BsmFI GGGAC 2 cut(s) 588, 890
BsmI GAATGC 1 cut(s) 938
BsnI GGCC 2 cut(s) 845, 1308
Bso31I GGTCTC 1 cut(s) 672
Bsp1286I GDGCHC 2 cut(s) 53, 589
Bsp1407I TGTACA 1 cut(s) 1128
Bsp143I GATC 8 cut(s) 66, 443, 624, 1009, 1030, 1153, 1362, 1454
BspACI CCGC 3 cut(s) 218, 846, 1309
BspANI GGCC 2 cut(s) 845, 1308
BspCNI CTCAG 4 cut(s) 286, 594, 823, 1267
BspFNI CGCG 1 cut(s) 402
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 4 cut(s) 202, 807, 953, 1155
BspMI ACCTGC 2 cut(s) 1054, 1109
BspPI GGATC 3 cut(s) 451, 1148, 1161
BspT107I GGYRCC 2 cut(s) 200, 951
BspTNI GGTCTC 1 cut(s) 672
BsrDI GCAATG 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 212
BsrGI TGTACA 1 cut(s) 1128
BsrI ACTGG 1 cut(s) 1226
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 3 cut(s) 240, 717, 798
BssMI GATC 8 cut(s) 66, 443, 624, 1009, 1030, 1153, 1362, 1454
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 2 cut(s) 744, 915
Bst4CI ACNGT 6 cut(s) 149, 347, 638, 799, 979, 1438
Bst6I CTCTTC 2 cut(s) 701, 1239
BstAUI TGTACA 1 cut(s) 1128
BstC8I GCNNGC 2 cut(s) 1088, 1378
BstDEI CTNAG 6 cut(s) 273, 334, 581, 810, 1016, 1254
BstDSI CCRYGG 1 cut(s) 798
BstENI CCTNNNNNAGG 1 cut(s) 960
BstF5I GGATG 5 cut(s) 387, 402, 690, 742, 940
BstFNI CGCG 1 cut(s) 402
BstHHI GCGC 3 cut(s) 402, 573, 1086
BstKTI GATC 8 cut(s) 69, 446, 627, 1012, 1033, 1156, 1365, 1457
BstMAI GTCTC 2 cut(s) 582, 672
BstMBI GATC 8 cut(s) 66, 443, 624, 1009, 1030, 1153, 1362, 1454
BstMCI CGRYCG 1 cut(s) 1309
BstMWI GCNNNNNNNGC 6 cut(s) 209, 218, 224, 842, 1197, 1206
BstNI CCWGG 2 cut(s) 744, 915
BstNSI RCATGY 3 cut(s) 668, 1185, 1345
BstSCI CCNGG 2 cut(s) 742, 913
BstSLI GKGCMC 1 cut(s) 589
BstUI CGCG 1 cut(s) 402
BstV1I GCAGC 3 cut(s) 813, 880, 1385
BstX2I RGATCY 2 cut(s) 1153, 1454
BstYI RGATCY 2 cut(s) 1153, 1454
BstZI CGGCCG 1 cut(s) 1306
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 2 cut(s) 845, 1308
BtgI CCRYGG 1 cut(s) 798
BtsCI GGATG 5 cut(s) 387, 402, 690, 742, 940
BtsI GCAGTG 2 cut(s) 1079, 1259
BtsIMutI CAGTG 5 cut(s) 154, 453, 589, 1079, 1259
BveI ACCTGC 2 cut(s) 1054, 1109
Cac8I GCNNGC 2 cut(s) 1088, 1378
CaiI CAGNNNCTG 1 cut(s) 1261
CciI TCATGA 1 cut(s) 63
CfoI GCGC 3 cut(s) 402, 573, 1086
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 1223
Csp6I GTAC 6 cut(s) 180, 348, 740, 755, 1129, 1439
CspCI CAANNNNNGTGG 2 cut(s) 1248, 1283
CviAII CATG 9 cut(s) 64, 99, 659, 665, 889, 946, 1028, 1182, 1342
CviQI GTAC 6 cut(s) 180, 348, 740, 755, 1129, 1439
DdeI CTNAG 6 cut(s) 273, 334, 581, 810, 1016, 1254
DpnI GATC 8 cut(s) 68, 445, 626, 1011, 1032, 1155, 1364, 1456
DpnII GATC 8 cut(s) 66, 443, 624, 1009, 1030, 1153, 1362, 1454
EaeI YGGCCR 2 cut(s) 843, 1306
EagI CGGCCG 1 cut(s) 1306
Eam1104I CTCTTC 2 cut(s) 701, 1239
EarI CTCTTC 2 cut(s) 701, 1239
EclXI CGGCCG 1 cut(s) 1306
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 672
Eco47I GGWCC 1 cut(s) 1223
Eco52I CGGCCG 1 cut(s) 1306
Eco57I CTGAAG 1 cut(s) 744
EcoNI CCTNNNNNAGG 1 cut(s) 960
EcoRII CCWGG 2 cut(s) 742, 913
EcoT14I CCWWGG 1 cut(s) 240
EcoT22I ATGCAT 1 cut(s) 940
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 9 cut(s) 67, 102, 662, 668, 892, 949, 1031, 1185, 1345
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 2 cut(s) 588, 890
FatI CATG 9 cut(s) 63, 98, 658, 664, 888, 945, 1027, 1181, 1341
FauNDI CATATG 1 cut(s) 526
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 6 cut(s) 219, 827, 846, 894, 1309, 1374
FokI GGATG 5 cut(s) 374, 409, 677, 749, 947
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 6 cut(s) 219, 827, 846, 894, 1309, 1374
FspBI CTAG 2 cut(s) 768, 1486
FspI TGCGCA 2 cut(s) 572, 1085
GlaI GCGC 3 cut(s) 401, 572, 1085
GluI GCNGC 6 cut(s) 219, 827, 846, 894, 1309, 1374
GsaI CCCAGC 2 cut(s) 50, 363
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 2 cut(s) 845, 1308
HapII CCGG 4 cut(s) 161, 204, 213, 1151
HhaI GCGC 3 cut(s) 402, 573, 1086
Hin1II CATG 9 cut(s) 67, 102, 662, 668, 892, 949, 1031, 1185, 1345
Hin6I GCGC 3 cut(s) 400, 571, 1084
HinP1I GCGC 3 cut(s) 400, 571, 1084
HinfI GANTC 5 cut(s) 260, 415, 814, 1067, 1354
HpaII CCGG 4 cut(s) 161, 204, 213, 1151
HphI GGTGA 6 cut(s) 499, 678, 785, 1140, 1323, 1415
Hpy166II GTNNAC 1 cut(s) 587
Hpy188I TCNGA 4 cut(s) 276, 813, 869, 991
Hpy188III TCNNGA 2 cut(s) 64, 1366
Hpy8I GTNNAC 1 cut(s) 587
HpyAV CCTTC 7 cut(s) 271, 653, 693, 768, 850, 1338, 1400
HpyCH4III ACNGT 6 cut(s) 149, 347, 638, 799, 979, 1438
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 218, 224, 842, 1197, 1206
HpyF3I CTNAG 6 cut(s) 273, 334, 581, 810, 1016, 1254
Hsp92II CATG 9 cut(s) 67, 102, 662, 668, 892, 949, 1031, 1185, 1345
HspAI GCGC 3 cut(s) 400, 571, 1084
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 8 cut(s) 66, 443, 624, 1009, 1030, 1153, 1362, 1454
LmnI GCTCC 2 cut(s) 535, 811
Lsp1109I GCAGC 3 cut(s) 813, 880, 1385
LweI GCATC 7 cut(s) 259, 387, 925, 1030, 1154, 1218, 1389
MaeI CTAG 2 cut(s) 768, 1486
MaeIII GTNAC 3 cut(s) 128, 448, 638
MalI GATC 8 cut(s) 68, 445, 626, 1011, 1032, 1155, 1364, 1456
MboI GATC 8 cut(s) 66, 443, 624, 1009, 1030, 1153, 1362, 1454
MboII GAAGA 5 cut(s) 52, 688, 992, 1004, 1226
MfeI CAATTG 2 cut(s) 247, 264
MflI RGATCY 2 cut(s) 1153, 1454
MhlI GDGCHC 2 cut(s) 53, 589
MluCI AATT 5 cut(s) 247, 264, 431, 921, 1057
MlyI GAGTC 2 cut(s) 269, 409
Mph1103I ATGCAT 1 cut(s) 940
MseI TTAA 4 cut(s) 458, 959, 1248, 1313
MslI CAYNNNNRTG 1 cut(s) 663
MspA1I CMGCKG 2 cut(s) 826, 1376
MspI CCGG 4 cut(s) 161, 204, 213, 1151
MspR9I CCNGG 2 cut(s) 744, 915
MunI CAATTG 2 cut(s) 247, 264
Mva1269I GAATGC 1 cut(s) 938
MvaI CCWGG 2 cut(s) 744, 915
MvnI CGCG 1 cut(s) 402
MwoI GCNNNNNNNGC 6 cut(s) 209, 218, 224, 842, 1197, 1206
NdeI CATATG 1 cut(s) 526
NdeII GATC 8 cut(s) 66, 443, 624, 1009, 1030, 1153, 1362, 1454
NlaIII CATG 9 cut(s) 67, 102, 662, 668, 892, 949, 1031, 1185, 1345
NlaIV GGNNCC 4 cut(s) 202, 807, 953, 1155
NmuCI GTSAC 2 cut(s) 448, 638
NsbI TGCGCA 2 cut(s) 572, 1085
NsiI ATGCAT 1 cut(s) 940
NspI RCATGY 3 cut(s) 668, 1185, 1345
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 2 cut(s) 1054, 1109
PciI ACATGT 1 cut(s) 664
PctI GAATGC 1 cut(s) 938
PfeI GAWTC 3 cut(s) 814, 1067, 1354
PkrI GCNGC 6 cut(s) 220, 828, 847, 895, 1310, 1375
PleI GAGTC 2 cut(s) 268, 409
PpsI GAGTC 2 cut(s) 268, 409
PscI ACATGT 1 cut(s) 664
PshBI ATTAAT 1 cut(s) 458
PsiI TTATAA 1 cut(s) 1397
Psp6I CCWGG 2 cut(s) 742, 913
PspFI CCCAGC 2 cut(s) 46, 359
PspGI CCWGG 2 cut(s) 742, 913
PspN4I GGNNCC 4 cut(s) 202, 807, 953, 1155
PspPI GGNCC 1 cut(s) 1223
PstNI CAGNNNCTG 1 cut(s) 1261
PsuI RGATCY 2 cut(s) 1153, 1454
PvuII CAGCTG 2 cut(s) 826, 1376
RsaI GTAC 6 cut(s) 181, 349, 741, 756, 1130, 1440
RsaNI GTAC 6 cut(s) 180, 348, 740, 755, 1129, 1439
RseI CAYNNNNRTG 1 cut(s) 663
SaqAI TTAA 4 cut(s) 458, 959, 1248, 1313
SatI GCNGC 6 cut(s) 219, 827, 846, 894, 1309, 1374
Sau3AI GATC 8 cut(s) 66, 443, 624, 1009, 1030, 1153, 1362, 1454
Sau96I GGNCC 1 cut(s) 1223
SchI GAGTC 2 cut(s) 269, 409
ScrFI CCNGG 2 cut(s) 744, 915
SduI GDGCHC 2 cut(s) 53, 589
SfaNI GCATC 7 cut(s) 259, 387, 925, 1030, 1154, 1218, 1389
SinI GGWCC 1 cut(s) 1223
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 1366
SmoI CTYRAG 1 cut(s) 1366
Sse9I AATT 5 cut(s) 247, 264, 431, 921, 1057
SsiI CCGC 3 cut(s) 218, 846, 1309
SspMI CTAG 2 cut(s) 768, 1486
StyD4I CCNGG 2 cut(s) 742, 913
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 6 cut(s) 149, 347, 638, 799, 979, 1438
TaqI TCGA 2 cut(s) 462, 1357
TasI AATT 5 cut(s) 247, 264, 431, 921, 1057
TatI WGTACW 2 cut(s) 347, 1128
TauI GCSGC 3 cut(s) 221, 848, 1311
TfiI GAWTC 3 cut(s) 814, 1067, 1354
Tru1I TTAA 4 cut(s) 458, 959, 1248, 1313
Tru9I TTAA 4 cut(s) 458, 959, 1248, 1313
TscAI CASTG 5 cut(s) 154, 453, 589, 1086, 1266
TseFI GTSAC 2 cut(s) 448, 638
TseI GCWGC 3 cut(s) 826, 893, 1373
Tsp45I GTSAC 2 cut(s) 448, 638
TspDTI ATGAA 6 cut(s) 30, 52, 188, 376, 1206, 1457
TspGWI ACGGA 2 cut(s) 995, 1236
TspRI CASTG 5 cut(s) 154, 453, 589, 1086, 1266
VneI GTGCAC 1 cut(s) 585
VpaK11BI GGWCC 1 cut(s) 1223
VspI ATTAAT 1 cut(s) 458
XagI CCTNNNNNAGG 1 cut(s) 960
XceI RCATGY 3 cut(s) 668, 1185, 1345
XcmI CCANNNNNNNNNTGG 1 cut(s) 1111
XspI CTAG 2 cut(s) 768, 1486
Zsp2I ATGCAT 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.