MD05G1112000.v1.1

Squalene monooxygenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000108
Physical Location & Seq
Reverse (-)
22908492 .. 22911688
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1112000.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGATTACCAGTATGTTCTTTGTGGGGTGTTGGTTTCTTTGCTTGGGTCGGTTTTCTTCATGATCATCAACACCACTCTCAGCGGCGAGAAGAAGAAGGTCGTCGAGAAAGTTTCCAACGGCGTTAACGGAAATGGGTACGTGTGGATGCCGTCCCAGAACGGCAGTTTTGTTCCGGATATTGCCAGGGGAACGGACGTTGTCATTGTCGGTGCCGGAGTTGCCGGTGCTGCCCTTGCTTACACTCTTGGAAAGGAAGGACGCCGCGTACATGTGATCGAAAGAGATTTGAGTGAGCCAGACAGAATTGTTGGTGAGCTGTTGCAGCCTGGAGGCTATCTCAAATTGATTGAGTTGGGTCTTGAGGACTGTGCAAATGAGTCCATTGATGCTCAGAAAGTGTTTGGTTATGCTCTTTACAAAAATGGAAATGCCACAAAACTATCTTATCCCTTGGAAAACTATACTTCAGATATCGCCGGGAGAAGTTTTCATAATGGGCGTTTCATCCAAAGAATGCGTGAGAGGGTTGCAACTCTTAAAAACGTGACACTAGAACAAGGAACTGTGACAACCCTAATTGAAGAAAAGGGCACCATCAAGGGTGTGATGTACAAGAACAAGGCTGGAGAGGAGATGAGATCATATGCTCCACTAACAATAGTATGTGATGGGTGCTTTTCAAATCTCCGCCGCAATCTCTGTACTCCTAACGTTGAAAATCCTTCTTGCTTCGTTGGTTTGATCTTGGAGAATTGTGACCTTCCTCATGCAAACCACGGACATGTGATTTTGGGAGACCCTTCACCTATCTTATTTTACCCCATTAGCGGTACCGAAGTTCGTTGTTTGGTAGATGTTCCCGGCACAAAAGTACCTTCGGTGGCTAATGGTGAAATGGCCAAGTACTTGAAAAATGTGGTTGCTCCCCAGGTTCCCCCTCAGCTCTTGAAGGCTTTTCTAGCCGCAGTTGAGAAAGGAAACATCAGAACAATGCAAAACAAAAGCATGGCTGCTCATCCTCAACCCACTCCCGGTGCAATTTTATTAGGGGATGCATTCAACATGAGACATCCTTTAACCGGAGGAGGAATGACCGTGGCTCTTTCCGACATTGTTCTTCTAAGAGATCTTCTTCGACCCCTAACTGATCTCAACGACGCACCAGCTTTGTGCGAATATCTAGAATCATTCTACACACTTCGCAAGCCTGTGTCATCTACCATAAACACGTTGGCTGGTGCCTTGTACAAGGTGTTTTGTGCATCACCTGACCCGGCAAGACAAGAAATGAGGGAAGCTTGTTTTGACTATTTGAGCCTGGGAGGCTTATGTACAAGGGGACCAATATCTTTGCTCTCTGGCCTTAACCCTCGCCCGATACACCTGTTTCTCCATTTCTTTGCCGTGGCTGTCTATGGAGTAGGCCGCTTAATGATTCCATTCCCTACGCCTAAACGCGTATGGCTTGCGACTAGATTGCTCTTGGGTGCATCGGGAATTATATTCCCCATTATTAAGGGTGAAGGAGTTAGGCAAATGTTCTTTCCTGCAACAATCCCGGCATATTACAGGGCTCCTCCTCTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

531

Amino Acids

57.75

Weight (kDa)

8.59

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 65 - 376 1.5e-16 FAD binding domain
DAO PF01266 66 - 96 1.1e-06 FAD dependent oxidoreductase
SE PF08491 217 - 489 1.7e-119 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 833
AccB1I GGYRCC 4 cut(s) 212, 593, 833, 1241
AccII CGCG 2 cut(s) 267, 1461
AccIII TCCGGA 1 cut(s) 175
AciI CCGC 7 cut(s) 84, 265, 691, 694, 831, 966, 1429
AclI AACGTT 1 cut(s) 714
AcoI YGGCCR 1 cut(s) 900
AcuI CTGAAG 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 262
AfaI GTAC 9 cut(s) 140, 270, 614, 706, 835, 876, 908, 1250, 1336
AfiI CCNNNNNNNGG 3 cut(s) 830, 1034, 1082
AflIII ACRYGT 5 cut(s) 141, 271, 784, 1230, 1459
AgsI TTSAA 7 cut(s) 584, 684, 719, 913, 952, 1063, 1589
AjnI CCWGG 4 cut(s) 185, 328, 930, 1320
AluBI AGCT 4 cut(s) 319, 946, 1169, 1301
AluI AGCT 4 cut(s) 319, 946, 1169, 1301
Alw26I GTCTC 2 cut(s) 792, 1063
Aor13HI TCCGGA 1 cut(s) 175
AoxI GGCC 3 cut(s) 900, 1363, 1426
ApeKI GCWGC 3 cut(s) 230, 325, 1013
Asp718I GGTACC 1 cut(s) 833
AspS9I GGNCC 1 cut(s) 1343
AsuC2I CCSGG 5 cut(s) 481, 864, 1035, 1277, 1562
AsuHPI GGTGA 5 cut(s) 326, 798, 905, 1260, 1535
AvaII GGWCC 1 cut(s) 1343
BaeGI GKGCMC 1 cut(s) 596
BaeI ACNNNNGTAYC 6 cut(s) 825, 858, 858, 891, 1373, 1406
BalI TGGCCA 1 cut(s) 902
BanI GGYRCC 4 cut(s) 212, 593, 833, 1241
BanII GRGCYC 1 cut(s) 1579
BbvCI CCTCAGC 1 cut(s) 942
BbvI GCAGC 3 cut(s) 217, 337, 1000
BccI CCATC 2 cut(s) 605, 665
BceAI ACGGC 4 cut(s) 136, 136, 178, 1391
BcgI CGANNNNNNTGC 2 cut(s) 1462, 1496
BciT130I CCWGG 4 cut(s) 187, 330, 932, 1322
BclI TGATCA 1 cut(s) 63
BcnI CCSGG 5 cut(s) 481, 864, 1035, 1277, 1562
BcoDI GTCTC 2 cut(s) 792, 1063
BfaI CTAG 4 cut(s) 554, 962, 1184, 1476
BglI GCCNNNNNGGC 1 cut(s) 1326
BglII AGATCT 1 cut(s) 1129
BisI GCNGC 8 cut(s) 85, 231, 265, 326, 694, 966, 1014, 1429
BlsI GCNGC 8 cut(s) 86, 232, 266, 327, 695, 967, 1015, 1430
BmcAI AGTACT 1 cut(s) 908
Bme1390I CCNGG 9 cut(s) 187, 330, 481, 864, 932, 1035, 1277, 1322, 1562
Bme18I GGWCC 1 cut(s) 1343
BmgT120I GGNCC 1 cut(s) 1343
BmiI GGNNCC 7 cut(s) 214, 595, 835, 936, 1243, 1344, 1578
BmrFI CCNGG 9 cut(s) 187, 330, 481, 864, 932, 1035, 1277, 1322, 1562
BmsI GCATC 5 cut(s) 138, 379, 1045, 1274, 1502
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BpmI CTGGAG 2 cut(s) 351, 648
Bpu10I CCTNAGC 1 cut(s) 942
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 5 cut(s) 481, 864, 1035, 1277, 1562
BsaAI YACGTR 1 cut(s) 142
BsaHI GRCGYC 1 cut(s) 262
BsaI GGTCTC 1 cut(s) 792
BsaJI CCNNGG 7 cut(s) 186, 453, 778, 930, 1098, 1321, 1407
BsaWI WCCGGW 2 cut(s) 175, 1082
Bsc4I CCNNNNNNNGG 3 cut(s) 830, 1034, 1082
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 1 cut(s) 10
BseAI TCCGGA 1 cut(s) 175
BseBI CCWGG 4 cut(s) 187, 330, 932, 1322
BseDI CCNNGG 7 cut(s) 186, 453, 778, 930, 1098, 1321, 1407
BseGI GGATG 5 cut(s) 153, 507, 1018, 1060, 1072
BseLI CCNNNNNNNGG 3 cut(s) 830, 1034, 1082
BseMII CTCAG 3 cut(s) 94, 407, 956
BseNI ACTGG 1 cut(s) 10
BseRI GAGGAG 4 cut(s) 647, 1101, 1569, 1572
BseSI GKGCMC 1 cut(s) 596
BseXI GCAGC 3 cut(s) 217, 337, 1000
Bsh1236I CGCG 2 cut(s) 267, 1461
BshFI GGCC 3 cut(s) 902, 1365, 1428
BshNI GGYRCC 4 cut(s) 212, 593, 833, 1241
BsiSI CCGG 9 cut(s) 176, 216, 225, 480, 864, 1035, 1083, 1277, 1562
BslFI GGGAC 2 cut(s) 139, 1356
BslI CCNNNNNNNGG 3 cut(s) 830, 1034, 1082
BsmAI GTCTC 2 cut(s) 792, 1063
BsmFI GGGAC 2 cut(s) 139, 1356
BsmI GAATGC 2 cut(s) 522, 1058
BsnI GGCC 3 cut(s) 902, 1365, 1428
Bso31I GGTCTC 1 cut(s) 792
Bsp1286I GDGCHC 2 cut(s) 596, 1579
Bsp13I TCCGGA 1 cut(s) 175
Bsp1407I TGTACA 3 cut(s) 612, 1248, 1334
Bsp143I GATC 6 cut(s) 63, 276, 641, 744, 1129, 1150
BspACI CCGC 7 cut(s) 84, 265, 691, 694, 831, 966, 1429
BspANI GGCC 3 cut(s) 902, 1365, 1428
BspCNI CTCAG 3 cut(s) 93, 406, 955
BspEI TCCGGA 1 cut(s) 175
BspFNI CGCG 2 cut(s) 267, 1461
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 7 cut(s) 214, 595, 835, 936, 1243, 1344, 1578
BspT107I GGYRCC 4 cut(s) 212, 593, 833, 1241
BspTNI GGTCTC 1 cut(s) 792
BsrFI RCCGGY 1 cut(s) 224
BsrGI TGTACA 3 cut(s) 612, 1248, 1334
BsrI ACTGG 1 cut(s) 10
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 7 cut(s) 186, 453, 778, 930, 1098, 1321, 1407
BssMI GATC 6 cut(s) 63, 276, 641, 744, 1129, 1150
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 4 cut(s) 187, 330, 932, 1322
Bst4CI ACNGT 3 cut(s) 371, 568, 1099
BstACI GRCGYC 1 cut(s) 262
BstAUI TGTACA 3 cut(s) 612, 1248, 1334
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 1208, 1470
BstDEI CTNAG 4 cut(s) 80, 393, 942, 1124
BstDSI CCRYGG 3 cut(s) 778, 1098, 1407
BstF5I GGATG 5 cut(s) 153, 507, 1018, 1060, 1072
BstFNI CGCG 2 cut(s) 267, 1461
BstKTI GATC 6 cut(s) 66, 279, 644, 747, 1132, 1153
BstMAI GTCTC 2 cut(s) 792, 1063
BstMBI GATC 6 cut(s) 63, 276, 641, 744, 1129, 1150
BstMWI GCNNNNNNNGC 6 cut(s) 221, 230, 236, 325, 962, 1326
BstNI CCWGG 4 cut(s) 187, 330, 932, 1322
BstNSI RCATGY 2 cut(s) 275, 788
BstSCI CCNGG 9 cut(s) 185, 328, 479, 862, 930, 1033, 1275, 1320, 1560
BstSLI GKGCMC 1 cut(s) 596
BstUI CGCG 2 cut(s) 267, 1461
BstV1I GCAGC 3 cut(s) 217, 337, 1000
BstX2I RGATCY 1 cut(s) 1129
BstYI RGATCY 1 cut(s) 1129
BsuRI GGCC 3 cut(s) 902, 1365, 1428
BtgI CCRYGG 3 cut(s) 778, 1098, 1407
BtsCI GGATG 5 cut(s) 153, 507, 1018, 1060, 1072
Cac8I GCNNGC 2 cut(s) 1208, 1470
CciI TCATGA 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 1 cut(s) 1343
CseI GACGC 2 cut(s) 270, 1169
Csp6I GTAC 9 cut(s) 139, 269, 613, 705, 834, 875, 907, 1249, 1335
CviAII CATG 6 cut(s) 61, 272, 770, 785, 1009, 1066
CviQI GTAC 9 cut(s) 139, 269, 613, 705, 834, 875, 907, 1249, 1335
DdeI CTNAG 4 cut(s) 80, 393, 942, 1124
DpnI GATC 6 cut(s) 65, 278, 643, 746, 1131, 1152
DpnII GATC 6 cut(s) 63, 276, 641, 744, 1129, 1150
EaeI YGGCCR 1 cut(s) 900
EciI GGCGGA 1 cut(s) 680
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 1579
Eco31I GGTCTC 1 cut(s) 792
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 1343
Eco57I CTGAAG 1 cut(s) 453
EcoRII CCWGG 4 cut(s) 185, 328, 930, 1320
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 1 cut(s) 453
EcoT22I ATGCAT 1 cut(s) 1060
EcoT38I GRGCYC 1 cut(s) 1579
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 6 cut(s) 64, 275, 773, 788, 1012, 1069
FaqI GGGAC 2 cut(s) 139, 1356
FatI CATG 6 cut(s) 60, 271, 769, 784, 1008, 1065
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 8 cut(s) 85, 231, 265, 326, 694, 966, 1014, 1429
FokI GGATG 5 cut(s) 160, 494, 1005, 1059, 1067
FriOI GRGCYC 1 cut(s) 1579
Fsp4HI GCNGC 8 cut(s) 85, 231, 265, 326, 694, 966, 1014, 1429
FspBI CTAG 4 cut(s) 554, 962, 1184, 1476
GluI GCNGC 8 cut(s) 85, 231, 265, 326, 694, 966, 1014, 1429
GsuI CTGGAG 2 cut(s) 351, 648
HaeIII GGCC 3 cut(s) 902, 1365, 1428
HapII CCGG 9 cut(s) 176, 216, 225, 480, 864, 1035, 1083, 1277, 1562
HgaI GACGC 2 cut(s) 270, 1169
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 6 cut(s) 64, 275, 773, 788, 1012, 1069
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 1299
HinfI GANTC 3 cut(s) 380, 1187, 1438
HpaI GTTAAC 1 cut(s) 127
HpaII CCGG 9 cut(s) 176, 216, 225, 480, 864, 1035, 1083, 1277, 1562
HphI GGTGA 5 cut(s) 326, 798, 905, 1260, 1535
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 4 cut(s) 396, 472, 989, 1111
Hpy188III TCNNGA 8 cut(s) 61, 106, 176, 362, 949, 1184, 1497, 1586
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 2 cut(s) 107, 1163
HpyAV CCTTC 8 cut(s) 91, 251, 735, 773, 813, 888, 946, 1520
HpyCH4III ACNGT 3 cut(s) 371, 568, 1099
HpyCH4IV ACGT 5 cut(s) 141, 198, 546, 714, 1232
HpyF10VI GCNNNNNNNGC 6 cut(s) 221, 230, 236, 325, 962, 1326
HpyF3I CTNAG 4 cut(s) 80, 393, 942, 1124
HpySE526I ACGT 5 cut(s) 141, 198, 546, 714, 1232
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 6 cut(s) 64, 275, 773, 788, 1012, 1069
Kpn2I TCCGGA 1 cut(s) 175
KpnI GGTACC 1 cut(s) 837
Ksp22I TGATCA 1 cut(s) 63
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 6 cut(s) 63, 276, 641, 744, 1129, 1150
LmnI GCTCC 3 cut(s) 655, 931, 1582
Lsp1109I GCAGC 3 cut(s) 217, 337, 1000
LweI GCATC 5 cut(s) 138, 379, 1045, 1274, 1502
MaeI CTAG 4 cut(s) 554, 962, 1184, 1476
MaeII ACGT 5 cut(s) 141, 198, 546, 714, 1232
MaeIII GTNAC 3 cut(s) 547, 568, 758
MalI GATC 6 cut(s) 65, 278, 643, 746, 1131, 1152
MboI GATC 6 cut(s) 63, 276, 641, 744, 1129, 1150
MboII GAAGA 7 cut(s) 49, 103, 106, 596, 1112, 1124, 1127
MflI RGATCY 1 cut(s) 1129
MhlI GDGCHC 2 cut(s) 596, 1579
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 6 cut(s) 306, 344, 579, 754, 1041, 1500
MluI ACGCGT 1 cut(s) 1459
MluNI TGGCCA 1 cut(s) 902
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 2 cut(s) 141, 1134
Mox20I TGGCCA 1 cut(s) 902
Mph1103I ATGCAT 1 cut(s) 1060
MroI TCCGGA 1 cut(s) 175
MscI TGGCCA 1 cut(s) 902
MseI TTAA 6 cut(s) 126, 540, 1079, 1368, 1433, 1518
MslI CAYNNNNRTG 1 cut(s) 783
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 9 cut(s) 176, 216, 225, 480, 864, 1035, 1083, 1277, 1562
MspR9I CCNGG 9 cut(s) 187, 330, 481, 864, 932, 1035, 1277, 1322, 1562
Mva1269I GAATGC 2 cut(s) 522, 1058
MvaI CCWGG 4 cut(s) 187, 330, 932, 1322
MvnI CGCG 2 cut(s) 267, 1461
MwoI GCNNNNNNNGC 6 cut(s) 221, 230, 236, 325, 962, 1326
NciI CCSGG 5 cut(s) 481, 864, 1035, 1277, 1562
NdeI CATATG 1 cut(s) 646
NdeII GATC 6 cut(s) 63, 276, 641, 744, 1129, 1150
NlaIII CATG 6 cut(s) 64, 275, 773, 788, 1012, 1069
NlaIV GGNNCC 7 cut(s) 214, 595, 835, 936, 1243, 1344, 1578
NmuCI GTSAC 3 cut(s) 547, 568, 758
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 2 cut(s) 275, 788
PagI TCATGA 1 cut(s) 60
PciI ACATGT 2 cut(s) 271, 784
PctI GAATGC 2 cut(s) 522, 1058
PfeI GAWTC 2 cut(s) 1187, 1438
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 8 cut(s) 86, 232, 266, 327, 695, 967, 1015, 1430
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
Ppu21I YACGTR 1 cut(s) 142
PscI ACATGT 2 cut(s) 271, 784
Psp1406I AACGTT 1 cut(s) 714
Psp6I CCWGG 4 cut(s) 185, 328, 930, 1320
PspGI CCWGG 4 cut(s) 185, 328, 930, 1320
PspN4I GGNNCC 7 cut(s) 214, 595, 835, 936, 1243, 1344, 1578
PspPI GGNCC 1 cut(s) 1343
PsuI RGATCY 1 cut(s) 1129
PsyI GACNNNGTC 1 cut(s) 200
RsaI GTAC 9 cut(s) 140, 270, 614, 706, 835, 876, 908, 1250, 1336
RsaNI GTAC 9 cut(s) 139, 269, 613, 705, 834, 875, 907, 1249, 1335
RseI CAYNNNNRTG 1 cut(s) 783
SaqAI TTAA 6 cut(s) 126, 540, 1079, 1368, 1433, 1518
SatI GCNGC 8 cut(s) 85, 231, 265, 326, 694, 966, 1014, 1429
Sau3AI GATC 6 cut(s) 63, 276, 641, 744, 1129, 1150
Sau96I GGNCC 1 cut(s) 1343
ScaI AGTACT 1 cut(s) 908
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 9 cut(s) 187, 330, 481, 864, 932, 1035, 1277, 1322, 1562
SduI GDGCHC 2 cut(s) 596, 1579
SfaNI GCATC 5 cut(s) 138, 379, 1045, 1274, 1502
SinI GGWCC 1 cut(s) 1343
SmiMI CAYNNNNRTG 1 cut(s) 783
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 6 cut(s) 306, 344, 579, 754, 1041, 1500
SsiI CCGC 7 cut(s) 84, 265, 691, 694, 831, 966, 1429
SspMI CTAG 4 cut(s) 554, 962, 1184, 1476
StyD4I CCNGG 9 cut(s) 185, 328, 479, 862, 930, 1033, 1275, 1320, 1560
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 3 cut(s) 371, 568, 1099
TaiI ACGT 5 cut(s) 144, 201, 549, 717, 1235
TaqI TCGA 3 cut(s) 105, 279, 1138
TasI AATT 6 cut(s) 306, 344, 579, 754, 1041, 1500
TatI WGTACW 5 cut(s) 612, 704, 906, 1248, 1334
TauI GCSGC 5 cut(s) 87, 267, 696, 968, 1431
TfiI GAWTC 2 cut(s) 1187, 1438
Tru1I TTAA 6 cut(s) 126, 540, 1079, 1368, 1433, 1518
Tru9I TTAA 6 cut(s) 126, 540, 1079, 1368, 1433, 1518
TseFI GTSAC 3 cut(s) 547, 568, 758
TseI GCWGC 3 cut(s) 230, 325, 1013
Tsp45I GTSAC 3 cut(s) 547, 568, 758
TspDTI ATGAA 3 cut(s) 49, 482, 496
TspGWI ACGGA 3 cut(s) 144, 209, 795
Tth111I GACNNNGTC 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 1343
XbaI TCTAGA 1 cut(s) 1183
XceI RCATGY 2 cut(s) 275, 788
XcmI CCANNNNNNNNNTGG 1 cut(s) 1231
XspI CTAG 4 cut(s) 554, 962, 1184, 1476
ZrmI AGTACT 1 cut(s) 908
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.