Rw6G003100

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000108
Physical Location & Seq
Forward (+)
5005574 .. 5009599
4026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003100.1

Sequence Viewer

Length: 1758 bp
ATGGTGTCTTACGAGTACGTTCTGGGTGGAGTCTTGGCTTCTCTGCTCGGCTCTGTTTTCTTCATGATCATCACCAGCACTTTTAGCGCCAAGAAAAAGGTAGAGGCTTTCAAGAGTGATAATGGGTTTGTGAGGATGGCGCAGAACGGTGTGTTTCGACCGGAGATGGAGGAGGAGGAGAAAAGTACCGACGTCGTCATTGTCGGCGCCGGAGTTGCCGGTGCAGCTCTTGCTACACTCTTGCCAAGGTCAGATTGTAATGAAAATATGAAATCTCTTGAAGGACGCCGTGTACATGTTATTGAAAGAGACTTGAGTGAGCCGGACAGAATTGTTGGTGAGCTTTTGCAGCCTGGGGGTTATCTCAAGTTGATTGAGTTGGGTCTTGAAGACTGTGCAAACGAGTCAATTGATGCTCAGAAGGTCTTTGGTTATGCCCTTTACAAAGATGGCAAGGATACAAAACTATCTTATCCGTTGGAGCAGTATAGTTCAGATGTGGCTGGGAGAAGTTTCCACAATGGGCGGTTTATTCAAAGAATGCGTGAAAAAGCTGCAATTCTTTCAAATGTCAAATTGGAACAAGGATCGGTGACAACACTAATTGAGGAAAAGGGCATTGTCAAAGGGGTGATTTACAAGAACAAGGCTGGAGAGGAGATGAGAACGTATGCTCCACTAACAATCGTCTGTGATGGCTGCTTTTCAAATCTGCGTAAATCTCTCAGTGCTCCAAAGGTTGAAAGTCCCTCTTGTTTTGTTGGTTTGGTGTTAGAGAACTGTGATCTTCCACATGCAAATCATGGACATGTGATTTTGGGAGACCCTTCACCCATCCTGTTTTATCCTATCAGTTCCACTGAGATTCGTTGTTTGGTCGATGTACCTGGGACAAAAGTACCTTCAGTTGCTAGTGGTGAAATGGCCAACTATTTGAAGACTGTGGTGGCTCCTCAGGTTCCCCCACAGCTCTACAATGCTTTTATGGTTGCAGTAGAGAAAGGAAACATTAGATCCATGCAAAACAAAAGCATGGCTGCTAATCCTGTTCCCACTCCTGGTGCAATTTTGTTGGGGGATTCATTCAACATGAGGCATCCTTTAACAGGAGGAGGAATGACTGTGGCTCTTTCAGACATAGTTCTTCTCCGCGATCTTCTTAGACCCCTACGTGATCTTAATGATGCACCTGCTTTGTGCAATTACCTTGAATCATTTTATACATTGCGTAAGCCTGTGTCATCTACTATAAACACATTGGCAGGTGCATTGTACAAGGTGTTTTGTGCATCGCCCGATCCAGCAAGACAGGAAATGCGTGAAGCATGTTTTGGCTATTTGAGCCTTGGAGGAATCTGTTCATATGGACCAATATCTCTGCTCTCTGGCCTTAACCCTCGTCCGCTGCACTTGTTCCTCCATTTCTTTGCTGTTGCTATCTACGGTGTTGGGCGCTTAATGCTTCCATTCCCTTCACCACAACGCATGTGGCTTGGGTTCAGATTGATCTTGAGTGCATCAGGCATCATATTCCCCATTATAAAGGGAGAAGGAGTTAGACAGATGTTCTTTCCAGCAACGGTGCCAGCATATTATAGATCACCACCTACTAAATATATTAGAATTCTTCTAATAAATGATCTTACAAATGGCTTGGCTTCCCTATCTTTCTTTCATAATCTCCTGTTGATGATTGTCATTGTGCAGAACCATCTGAGCACAAGGTCTGCAGAAGTATCCCTGTTTTATCAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

585

Amino Acids

64.12

Weight (kDa)

8.35

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 63 - 384 4.8e-14 FAD binding domain
SE PF08491 225 - 497 2.4e-117 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1541
AarI CACCTGC 2 cut(s) 1198, 1253
AatII GACGTC 1 cut(s) 195
Acc36I ACCTGC 2 cut(s) 1198, 1253
AccB1I GGYRCC 2 cut(s) 206, 1582
AccII CGCG 1 cut(s) 1152
AciI CCGC 3 cut(s) 526, 1150, 1403
AclWI GGATC 3 cut(s) 595, 1008, 1292
AcoI YGGCCR 1 cut(s) 924
AcsI RAATTY 1 cut(s) 1623
AcuI CTGAAG 1 cut(s) 888
AcyI GRCGYC 3 cut(s) 192, 207, 286
AfaI GTAC 6 cut(s) 17, 187, 294, 885, 900, 1274
AfiI CCNNNNNNNGG 2 cut(s) 1058, 1106
AflIII ACRYGT 2 cut(s) 295, 808
AjnI CCWGG 3 cut(s) 352, 886, 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 1314, 1346
AloI GAACNNNNNNTCC 2 cut(s) 658, 690
AluBI AGCT 4 cut(s) 227, 343, 554, 970
AluI AGCT 4 cut(s) 227, 343, 554, 970
Alw21I GWGCWC 2 cut(s) 733, 1721
Alw26I GTCTC 2 cut(s) 303, 816
AlwI GGATC 3 cut(s) 595, 1008, 1292
AoxI GGCC 2 cut(s) 924, 1387
ApeKI GCWGC 6 cut(s) 224, 349, 554, 699, 1037, 1405
ApoI RAATTY 1 cut(s) 1623
Asp700I GAANNNNTTC 1 cut(s) 1357
AspLEI GCGC 4 cut(s) 89, 142, 209, 1455
AspS9I GGNCC 1 cut(s) 1367
AsuHPI GGTGA 8 cut(s) 64, 350, 604, 643, 822, 929, 1467, 1593
AvaII GGWCC 1 cut(s) 1367
AxyI CCTNAGG 1 cut(s) 954
BaeI ACNNNNGTAYC 2 cut(s) 882, 915
BalI TGGCCA 1 cut(s) 926
BanI GGYRCC 2 cut(s) 206, 1582
BbsI GAAGAC 2 cut(s) 396, 944
Bbv12I GWGCWC 2 cut(s) 733, 1721
BbvI GCAGC 6 cut(s) 236, 361, 541, 686, 1024, 1392
BccI CCATC 6 cut(s) 130, 160, 443, 689, 842, 1719
BceAI ACGGC 1 cut(s) 273
BciT130I CCWGG 3 cut(s) 354, 888, 1059
BciVI GTATCC 2 cut(s) 451, 1747
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 303, 816
BfaI CTAG 1 cut(s) 912
BfmI CTRYAG 1 cut(s) 1728
BfoI RGCGCY 3 cut(s) 90, 210, 1456
BfuAI ACCTGC 2 cut(s) 1198, 1253
BfuI GTATCC 2 cut(s) 451, 1747
BisI GCNGC 6 cut(s) 225, 350, 555, 700, 1038, 1406
BlsI GCNGC 6 cut(s) 226, 351, 556, 701, 1039, 1407
Bme1390I CCNGG 3 cut(s) 354, 888, 1059
Bme18I GGWCC 1 cut(s) 1367
BmgT120I GGNCC 1 cut(s) 1367
BmiI GGNNCC 4 cut(s) 208, 951, 960, 1584
BmrFI CCNGG 3 cut(s) 354, 888, 1059
BmsI GCATC 6 cut(s) 403, 1105, 1174, 1298, 1526, 1533
BpiI GAAGAC 2 cut(s) 396, 944
BpmI CTGGAG 1 cut(s) 672
BpuEI CTTGAG 3 cut(s) 334, 350, 1531
BsaAI YACGTR 1 cut(s) 1172
BsaHI GRCGYC 3 cut(s) 192, 207, 286
BsaI GGTCTC 1 cut(s) 816
BsaJI CCNNGG 4 cut(s) 245, 353, 887, 1345
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 4 cut(s) 658, 688, 1539, 1569
Bsc4I CCNNNNNNNGG 2 cut(s) 1058, 1106
Bse118I RCCGGY 1 cut(s) 218
Bse21I CCTNAGG 1 cut(s) 954
Bse3DI GCAATG 1 cut(s) 1223
BseBI CCWGG 3 cut(s) 354, 888, 1059
BseDI CCNNGG 4 cut(s) 245, 353, 887, 1345
BseGI GGATG 3 cut(s) 141, 834, 1096
BseLI CCNNNNNNNGG 2 cut(s) 1058, 1106
BseMI GCAATG 1 cut(s) 1223
BseMII CTCAG 5 cut(s) 431, 739, 852, 968, 1706
BseRI GAGGAG 6 cut(s) 185, 188, 191, 671, 942, 1125
BseXI GCAGC 6 cut(s) 236, 361, 541, 686, 1024, 1392
BseYI CCCAGC 1 cut(s) 503
BsgI GTGCAG 3 cut(s) 243, 1391, 1724
Bsh1236I CGCG 1 cut(s) 1152
Bsh1285I CGRYCG 1 cut(s) 161
BshFI GGCC 2 cut(s) 926, 1389
BshNI GGYRCC 2 cut(s) 206, 1582
BsiEI CGRYCG 1 cut(s) 161
BsiHKAI GWGCWC 2 cut(s) 733, 1721
BsiSI CCGG 4 cut(s) 161, 210, 219, 323
BslFI GGGAC 2 cut(s) 732, 904
BslI CCNNNNNNNGG 2 cut(s) 1058, 1106
BsmAI GTCTC 2 cut(s) 303, 816
BsmFI GGGAC 2 cut(s) 732, 904
BsmI GAATGC 1 cut(s) 546
BsnI GGCC 2 cut(s) 926, 1389
Bso31I GGTCTC 1 cut(s) 816
Bsp1286I GDGCHC 2 cut(s) 733, 1721
Bsp1407I TGTACA 2 cut(s) 292, 1272
BspACI CCGC 3 cut(s) 526, 1150, 1403
BspANI GGCC 2 cut(s) 926, 1389
BspCNI CTCAG 5 cut(s) 430, 738, 853, 967, 1707
BspFNI CGCG 1 cut(s) 1152
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 4 cut(s) 208, 951, 960, 1584
BspMAI CTGCAG 1 cut(s) 1732
BspMI ACCTGC 2 cut(s) 1198, 1253
BspPI GGATC 3 cut(s) 595, 1008, 1292
BspT107I GGYRCC 2 cut(s) 206, 1582
BspTNI GGTCTC 1 cut(s) 816
BsrDI GCAATG 1 cut(s) 1223
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 2 cut(s) 292, 1272
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 4 cut(s) 245, 353, 887, 1345
BssNI GRCGYC 3 cut(s) 192, 207, 286
BssT1I CCWWGG 2 cut(s) 245, 1345
Bst2UI CCWGG 3 cut(s) 354, 888, 1059
Bst4CI ACNGT 7 cut(s) 149, 395, 782, 943, 1123, 1445, 1582
BstACI GRCGYC 3 cut(s) 192, 207, 286
BstAPI GCANNNNNTGC 1 cut(s) 230
BstAUI TGTACA 2 cut(s) 292, 1272
BstBAI YACGTR 1 cut(s) 1172
BstC8I GCNNGC 1 cut(s) 1587
BstDEI CTNAG 6 cut(s) 417, 725, 861, 954, 1160, 1715
BstENI CCTNNNNNAGG 1 cut(s) 1104
BstF5I GGATG 3 cut(s) 141, 834, 1096
BstFNI CGCG 1 cut(s) 1152
BstH2I RGCGCY 3 cut(s) 90, 210, 1456
BstHHI GCGC 4 cut(s) 89, 142, 209, 1455
BstMAI GTCTC 2 cut(s) 303, 816
BstMCI CGRYCG 1 cut(s) 161
BstMWI GCNNNNNNNGC 7 cut(s) 84, 215, 224, 230, 349, 1341, 1459
BstNI CCWGG 3 cut(s) 354, 888, 1059
BstNSI RCATGY 5 cut(s) 299, 797, 812, 1329, 1489
BstSCI CCNGG 3 cut(s) 352, 886, 1057
BstSFI CTRYAG 1 cut(s) 1728
BstUI CGCG 1 cut(s) 1152
BstV1I GCAGC 6 cut(s) 236, 361, 541, 686, 1024, 1392
BstV2I GAAGAC 2 cut(s) 396, 944
BstX2I RGATCY 1 cut(s) 1013
BstYI RGATCY 1 cut(s) 1013
Bsu36I CCTNAGG 1 cut(s) 954
BsuI GTATCC 2 cut(s) 451, 1747
BsuRI GGCC 2 cut(s) 926, 1389
BtgZI GCGATG 1 cut(s) 1275
BtsCI GGATG 3 cut(s) 141, 834, 1096
BtsIMutI CAGTG 2 cut(s) 733, 858
BveI ACCTGC 2 cut(s) 1198, 1253
Cac8I GCNNGC 1 cut(s) 1587
CciI TCATGA 1 cut(s) 63
CfoI GCGC 4 cut(s) 89, 142, 209, 1455
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 1367
CseI GACGC 1 cut(s) 294
Csp6I GTAC 6 cut(s) 16, 186, 293, 884, 899, 1273
CspCI CAANNNNNGTGG 2 cut(s) 1469, 1504
CviQI GTAC 6 cut(s) 16, 186, 293, 884, 899, 1273
DdeI CTNAG 6 cut(s) 417, 725, 861, 954, 1160, 1715
DinI GGCGCC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 924
Eco130I CCWWGG 2 cut(s) 245, 1345
Eco31I GGTCTC 1 cut(s) 816
Eco47I GGWCC 1 cut(s) 1367
Eco57I CTGAAG 1 cut(s) 888
Eco81I CCTNAGG 1 cut(s) 954
EcoNI CCTNNNNNAGG 1 cut(s) 1104
EcoRI GAATTC 1 cut(s) 1623
EcoRII CCWGG 3 cut(s) 352, 886, 1057
EcoT14I CCWWGG 2 cut(s) 245, 1345
EgeI GGCGCC 1 cut(s) 208
EheI GGCGCC 1 cut(s) 208
ErhI CCWWGG 2 cut(s) 245, 1345
FalI AAGNNNNNCTT 2 cut(s) 736, 768
FaqI GGGAC 2 cut(s) 732, 904
FauNDI CATATG 1 cut(s) 1363
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 6 cut(s) 225, 350, 555, 700, 1038, 1406
FokI GGATG 3 cut(s) 148, 821, 1083
Fsp4HI GCNGC 6 cut(s) 225, 350, 555, 700, 1038, 1406
FspBI CTAG 1 cut(s) 912
GlaI GCGC 4 cut(s) 88, 141, 208, 1454
GluI GCNGC 6 cut(s) 225, 350, 555, 700, 1038, 1406
GsaI CCCAGC 1 cut(s) 507
GsuI CTGGAG 1 cut(s) 672
HaeII RGCGCY 3 cut(s) 90, 210, 1456
HaeIII GGCC 2 cut(s) 926, 1389
HapII CCGG 4 cut(s) 161, 210, 219, 323
HgaI GACGC 1 cut(s) 294
HhaI GCGC 4 cut(s) 89, 142, 209, 1455
Hin1I GRCGYC 3 cut(s) 192, 207, 286
Hin6I GCGC 4 cut(s) 87, 140, 207, 1453
HinP1I GCGC 4 cut(s) 87, 140, 207, 1453
HinfI GANTC 6 cut(s) 30, 404, 865, 1079, 1211, 1353
HpaII CCGG 4 cut(s) 161, 210, 219, 323
HphI GGTGA 8 cut(s) 64, 350, 604, 643, 822, 929, 1467, 1593
Hpy166II GTNNAC 1 cut(s) 293
Hpy188I TCNGA 6 cut(s) 253, 420, 496, 1135, 1502, 1716
Hpy188III TCNNGA 5 cut(s) 64, 112, 278, 386, 1510
Hpy8I GTNNAC 1 cut(s) 293
Hpy99I CGWCG 2 cut(s) 194, 197
HpyAV CCTTC 6 cut(s) 275, 415, 837, 912, 1482, 1544
HpyCH4III ACNGT 7 cut(s) 149, 395, 782, 943, 1123, 1445, 1582
HpyCH4IV ACGT 4 cut(s) 18, 192, 668, 1171
HpyF10VI GCNNNNNNNGC 7 cut(s) 84, 215, 224, 230, 349, 1341, 1459
HpyF3I CTNAG 6 cut(s) 417, 725, 861, 954, 1160, 1715
HpySE526I ACGT 4 cut(s) 18, 192, 668, 1171
Hsp92I GRCGYC 3 cut(s) 192, 207, 286
HspAI GCGC 4 cut(s) 87, 140, 207, 1453
KasI GGCGCC 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 66
LmnI GCTCC 4 cut(s) 481, 679, 736, 955
Lsp1109I GCAGC 6 cut(s) 236, 361, 541, 686, 1024, 1392
LweI GCATC 6 cut(s) 403, 1105, 1174, 1298, 1526, 1533
MaeI CTAG 1 cut(s) 912
MaeII ACGT 4 cut(s) 18, 192, 668, 1171
MaeIII GTNAC 1 cut(s) 592
MboII GAAGA 7 cut(s) 52, 401, 779, 949, 1136, 1148, 1619
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 1 cut(s) 1013
MhlI GDGCHC 2 cut(s) 733, 1721
MlsI TGGCCA 1 cut(s) 926
MluCI AATT 8 cut(s) 330, 408, 558, 575, 603, 1065, 1201, 1623
MluNI TGGCCA 1 cut(s) 926
Mly113I GGCGCC 1 cut(s) 207
MlyI GAGTC 2 cut(s) 39, 413
MmeI TCCRAC 1 cut(s) 459
Mox20I TGGCCA 1 cut(s) 926
MroXI GAANNNNTTC 1 cut(s) 1357
MscI TGGCCA 1 cut(s) 926
MseI TTAA 4 cut(s) 1103, 1179, 1392, 1457
MslI CAYNNNNRTG 1 cut(s) 807
Msp20I TGGCCA 1 cut(s) 926
MspA1I CMGCKG 1 cut(s) 1405
MspI CCGG 4 cut(s) 161, 210, 219, 323
MspR9I CCNGG 3 cut(s) 354, 888, 1059
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 1 cut(s) 546
MvaI CCWGG 3 cut(s) 354, 888, 1059
MvnI CGCG 1 cut(s) 1152
MwoI GCNNNNNNNGC 7 cut(s) 84, 215, 224, 230, 349, 1341, 1459
NarI GGCGCC 1 cut(s) 207
NdeI CATATG 1 cut(s) 1363
NlaIV GGNNCC 4 cut(s) 208, 951, 960, 1584
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 1 cut(s) 592
NspI RCATGY 5 cut(s) 299, 797, 812, 1329, 1489
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 2 cut(s) 1198, 1253
PciI ACATGT 2 cut(s) 295, 808
PctI GAATGC 1 cut(s) 546
PdmI GAANNNNTTC 1 cut(s) 1357
PfeI GAWTC 4 cut(s) 865, 1079, 1211, 1353
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 6 cut(s) 226, 351, 556, 701, 1039, 1407
PleI GAGTC 2 cut(s) 38, 412
PluTI GGCGCC 1 cut(s) 210
PpsI GAGTC 2 cut(s) 38, 412
Ppu21I YACGTR 1 cut(s) 1172
PscI ACATGT 2 cut(s) 295, 808
PsiI TTATAA 1 cut(s) 1541
Psp6I CCWGG 3 cut(s) 352, 886, 1057
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 3 cut(s) 352, 886, 1057
PspN4I GGNNCC 4 cut(s) 208, 951, 960, 1584
PspPI GGNCC 1 cut(s) 1367
PstI CTGCAG 1 cut(s) 1732
PsuI RGATCY 1 cut(s) 1013
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 6 cut(s) 17, 187, 294, 885, 900, 1274
RsaNI GTAC 6 cut(s) 16, 186, 293, 884, 899, 1273
RseI CAYNNNNRTG 1 cut(s) 807
SaqAI TTAA 4 cut(s) 1103, 1179, 1392, 1457
SatI GCNGC 6 cut(s) 225, 350, 555, 700, 1038, 1406
Sau96I GGNCC 1 cut(s) 1367
SchI GAGTC 2 cut(s) 39, 413
ScrFI CCNGG 3 cut(s) 354, 888, 1059
SduI GDGCHC 2 cut(s) 733, 1721
SfaNI GCATC 6 cut(s) 403, 1105, 1174, 1298, 1526, 1533
SfcI CTRYAG 1 cut(s) 1728
SfoI GGCGCC 1 cut(s) 208
SinI GGWCC 1 cut(s) 1367
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 3 cut(s) 313, 365, 1510
SmoI CTYRAG 3 cut(s) 313, 365, 1510
Sse9I AATT 8 cut(s) 330, 408, 558, 575, 603, 1065, 1201, 1623
SsiI CCGC 3 cut(s) 526, 1150, 1403
SspDI GGCGCC 1 cut(s) 206
SspMI CTAG 1 cut(s) 912
StyD4I CCNGG 3 cut(s) 352, 886, 1057
StyI CCWWGG 2 cut(s) 245, 1345
TaaI ACNGT 7 cut(s) 149, 395, 782, 943, 1123, 1445, 1582
TaiI ACGT 4 cut(s) 21, 195, 671, 1174
TaqI TCGA 2 cut(s) 157, 879
TasI AATT 8 cut(s) 330, 408, 558, 575, 603, 1065, 1201, 1623
TatI WGTACW 2 cut(s) 292, 1272
TfiI GAWTC 4 cut(s) 865, 1079, 1211, 1353
Tru1I TTAA 4 cut(s) 1103, 1179, 1392, 1457
Tru9I TTAA 4 cut(s) 1103, 1179, 1392, 1457
TscAI CASTG 2 cut(s) 733, 865
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 6 cut(s) 224, 349, 554, 699, 1037, 1405
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 6 cut(s) 52, 276, 284, 1071, 1350, 1664
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 2 cut(s) 733, 865
Tth111I GACNNNGTC 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 1367
XagI CCTNNNNNAGG 1 cut(s) 1104
XapI RAATTY 1 cut(s) 1623
XceI RCATGY 5 cut(s) 299, 797, 812, 1329, 1489
XcmI CCANNNNNNNNNTGG 1 cut(s) 1485
XmnI GAANNNNTTC 1 cut(s) 1357
XspI CTAG 1 cut(s) 912
ZraI GACGTC 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.