Rh2AG207100

Squalene epoxidase 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000108
Physical Location & Seq
Forward (+)
21031560 .. 21035614
4055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG207100.1

Sequence Viewer

Length: 1881 bp
ATGCAACTACAAAAGCCAATCTTCCAGACCCATCTTCCACCACAACCAAGCTCACAGCTTTTTGCTCAGATCCATCTCCATTACCATAAACCTCACCTTCCCACAATCTCTCTCTGCTTCCATAAACCCACATCAACAACACCCGAAAGCTCTACCAGTTCCAGCTGCATTCCAACAGAAACAGATAGGAGAACCAAACAGGGACATCTTTCTTCTTCTTCTTCATCTGGGTCTTGTAATTCATCGATTAATTCAATGATGGCGACAATGAAGGCGATCGATCCGTATGTTGTGTGGACGTTCTTTGCTTCTTTATTGGGTTTTCTTCTTCTCTCCCTTCTCCGCCGACGTAATTACGGTAATGCCACCGCCGAGGACAAGGGAATGGTTAAAAACAAGAACAAGACGACCGAGAATCATGTTTCTGAAGAATGTGTGAAGAGCTCCGGCAACGGAGACTTCCGGCAGGAGTTCGGCTCCGGAACTGACGTCATCATCGTTGGCGCCGGTGTTGCTGGCGCTGCTCTTGCACACACCCTTGGCAAGGAGGGAAGACGTGTTCGTGTGATTGAAAGAGACTTGACAGAGCCGGATCGAATTGTTGGCGAACTTCTCCAGCCTGGGGGATACCTGAAGCTGATTGACTTGGGACTTGAAGATTGTGTTGAGGAAATCGATGCTCAGCGAGTTCTCGGATATGCTCTTTTTAAGGATGGGAAGAATACTACACTGTCCTATCCCCTGGAACAGTTCCACTCGGATGTGGCGGGTAGAAGTTTTCACAATGGGCGATTCATACAGAGGATGAGAGAAAAAGCTGCTACTCTTCCCAATGTACAACTGGAGCAAGGTACAGTCACATCCCTGCTTGAAGAAAATGGGACAATTACGGGGGTTCAGTACAAAACTAAGGATGGTCAAGAACTTCAAGCTCATGCTCCTCTTACAATTGTCTGCGATGGTTGCTTCTCAAATCTGCGACGCACTCTTTGTAAGCCTCAGGTAGAAGTGCCCTCTTGTTTTGTGGGATTAATTTTGGAAAATTGTGAACTCCCATTTGCAAATCACGGGCATGTTATTCTTGCTGATCCATCTCCAATCCTGTTTTACCAAATCAGTAGTACAGAGGTCCGCTGTTTAGTTGATGTGCCTGGCCCGAAAGTACCTCCCATCACTAATGGAGCAATGGCTAACTATTTGAAGAATGTGGTAGCTCCACAGCTTCCACCTGAACTTCATGATCCCTTCATATCTGCAATTGACAAAGGAAGTATTAGAACTATGCCGAATAGAAGCATGCCAGCCAATCCGCAGCCAACTCCTGGAGCCCTTTTAATGGGTGATGCTTTCAATATGCGTCATCCTTTAACTGGTGGAGGAATGACTGTGGCACTATCGGACATTGTTATACTCAGGGATCTTCTTAAACCATTACGTGACATAAATGATGCCTCTTCATTGTGCAACTACCTAGAATCCTTTTATACCTTGCGCAAGCCAGTTGCATCTACAATAAACACCTTGGCAGGTGCCCTATACAAGGTGTTCTCTGCCTCCCCTGACCAAGCAAGGAAGGAGATGCGCCAAGCATGCTTTGACTATCTGAGCCTTGGAGGTGCCTGTTCAACTGGACCAGTGGCTCTGCTCTCTGGTCTAAATCCTCGCCCGCTGAGTTTAGTTCTCCATTTTTTTGCTGTTGCGATATATGGTGTTGGACGTTTGCTTCTACCATTTCCTTCACCGAAACGCATGTGGATTGGAGTGAGATTAATCTGGAGCGCAGTAGGCATAATATTTCCCATCATCAAGGCAGAAGGTGTGAGACAGATGTTCTTTCCTGCTACTATTCCAGCTTGTCATAGGGCTCCTCCTGCTAACTGA

Protein Analysis

626

Amino Acids

68.34

Weight (kDa)

8.62

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 162 - 479 5.8e-19 FAD binding domain
SE PF08491 312 - 585 4.8e-121 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22830 AT4G37760
fragaria_vesca FvH4_1g18300 FvH4_1g20540 FvH4_2g02580 FvH4_2g02590 FvH4_2g02590 FvH4_2g02590 FvH4_2g02600 FvH4_2g02650 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02652 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_2g02660 FvH4_4g14040 FvH4_4g14050
malus_domestica MD00G1080800.v1.1 MD02G1190300.v1.1 MD02G1272900.v1.1 MD05G1111800.v1.1 MD05G1112000.v1.1 MD05G1112100.v1.1 MD05G1112200.v1.1 MD07G1041500.v1.1 MD07G1041600.v1.1 MD07G1041700.v1.1 MD07G1041800.v1.1 MD07G1041900.v1.1 MD07G1042000.v1.1 MD10G1114800.v1.1 MD15G1301500.v1.1
prunus_persica Prupe.6G216100_v2.0.a1 Prupe.8G156600_v2.0.a1 Prupe.8G156800_v2.0.a1
pyrus_communis pycom02g15440 pycom02g15450 pycom02g23370 pycom02g23380 pycom05g10720 pycom05g10730 pycom05g10770 pycom07g02910 pycom07g02920 pycom07g02930 pycom07g02940 pycom07g02950 pycom07g02960 pycom10g09930 pycom15g26420 pycom15g26430
rosa_chinensis RchiOBHm_Chr1g0337291 RchiOBHm_Chr1g0337301 RchiOBHm_Chr2g0108881 RchiOBHm_Chr2g0108891 RchiOBHm_Chr2g0108921 RchiOBHm_Chr4g0411471 RchiOBHm_Chr4g0411481 RchiOBHm_Chr4g0411491 RchiOBHm_Chr4g0419301 RchiOBHm_Chr4g0419311 RchiOBHm_Chr4g0419381 RchiOBHm_Chr4g0419391 RchiOBHm_Chr4g0419471 RchiOBHm_Chr4g0419611 RchiOBHm_Chr6g0246411 RchiOBHm_Chr6g0246421 RchiOBHm_Chr6g0246521 RchiOBHm_Chr6g0246791 RchiOBHm_Chr6g0246801 RchiOBHm_Chr6g0246811 RchiOBHm_Chr6g0246841 RchiOBHm_Chr6g0246851 RchiOBHm_Chr6g0246941 RchiOBHm_Chr6g0246951 RchiOBHm_Chr6g0246961 RchiOBHm_Chr6g0246981 RchiOBHm_Chr6g0246991 RchiOBHm_Chr6g0247001 RchiOBHm_Chr7g0177171 RchiOBHm_Chr7g0177181 RchiOBHm_Chr7g0177191
rosa_laevigata RLG00000007810 RLG00000007811 RLG00000007817 RLG00000008351 RLG00000008352 RLG00000008353 RLG00000013274 RLG00000014276 RLG00000015230 RLG00000015231 RLG00000015232 RLG00000015236 RLG00000015237 RLG00000015238 RLG00000015239 RLG00000015240 RLG00000015244 RLG00000015245 RLG00000015246 RLG00000015247 RLG00000017694 RLG00000029286
rosa_multiflora Rmu_co7985368.1_g000001 Rmu_co8117354.1_g000001 Rmu_co8129060.1_g000001 Rmu_co8143900.1_g000001 Rmu_co8230369.1_g000001 Rmu_co8341411.1_g000001 Rmu_co8365495.1_g000001 Rmu_co8367749.1_g000001 Rmu_co8422277.1_g000001 Rmu_sc0000455.1_g000001 Rmu_sc0000989.1_g000003 Rmu_sc0001293.1_g000004 Rmu_sc0001293.1_g000005 Rmu_sc0001293.1_g000006 Rmu_sc0001293.1_g000007 Rmu_sc0001982.1_g000018 Rmu_sc0001982.1_g000019 Rmu_sc0002539.1_g000051 Rmu_sc0003387.1_g000001 Rmu_sc0004469.1_g000022 Rmu_sc0004830.1_g000004 Rmu_sc0004932.1_g000010 Rmu_sc0005310.1_g000017 Rmu_sc0006222.1_g000001 Rmu_sc0006767.1_g000002 Rmu_sc0006875.1_g000001 Rmu_sc0009831.1_g000001 Rmu_sc0009831.1_g000002 Rmu_sc0009831.1_g000003 Rmu_sc0010547.1_g000017 Rmu_sc0012369.1_g000005 Rmu_sc0012369.1_g000006 Rmu_sc0017606.1_g000010 Rmu_sc0018495.1_g000001 Rmu_sc0018495.1_g000002 Rmu_sc0024638.1_g000002 Rmu_sc0031581.1_g000001 Rmu_sc0032897.1_g000005 Rmu_sc0037307.1_g000001 Rmu_sc0038108.1_g000001 Rmu_sc0041017.1_g000001
rosa_roxburghii Rroxscaffold_176G00431650 Rroxscaffold_2G00134690 Rroxscaffold_2G00134720 Rroxscaffold_4G00314560 Rroxscaffold_4G00326020 Rroxscaffold_5G00356060 Rroxscaffold_5G00356070 Rroxscaffold_5G00362290 Rroxscaffold_5G00362330 Rroxscaffold_5G00362340 Rroxscaffold_5G00362440 Rroxscaffold_5G00362450 Rroxscaffold_7G00203370 Rroxscaffold_7G00214390 Rroxscaffold_7G00214400 Rroxscaffold_7G00214410 Rroxscaffold_7G00214430 Rroxscaffold_7G00214440 Rroxscaffold_7G00214450 Rroxscaffold_7G00214460 Rroxscaffold_7G00214500
rosa_rugosa Rorug01G0134000.1 Rorug01G0134100.1 Rorug01G0134200.1 Rorug02G0156600 Rorug02G0156700 Rorug04G0108500 Rorug04G0108600 Rorug04G0158200 Rorug04G0158500 Rorug04G0158600 Rorug05G0521200 Rorug05G0521300 Rorug05G0521300 Rorug05G0521500 Rorug05G0521600 Rorug05G0521700 Rorug05G0521800 Rorug05G0521900 Rorug06G0011500
rosa_samantha Rh1AG153200 Rh1AG153300 Rh1CG060800 Rh1CG144100 Rh1CG144200 Rh1DG064800 Rh1DG160800 Rh1DG160900 Rh1DG161000 Rh2AG207100 Rh2AG207200 Rh2DG213300 Rh2DG213400 Rh4AG168400 Rh4AG168500 Rh4AG220000 Rh4CG179900 Rh4CG232800 Rh4DG162800 Rh4DG219200 Rh4DG219300 Rh6AG035000 Rh6AG035900 Rh6AG036000 Rh6AG036200 Rh6AG036300 Rh6AG036600 Rh6BG030600 Rh6BG030700 Rh6BG030800 Rh6BG031000 Rh6BG031300 Rh6BG031400 Rh6CG031200 Rh6CG031500 Rh6CG031600 Rh6CG031700 Rh6CG031800 Rh6CG031900 Rh6CG032000 Rh7AG003800
rosa_wichuraiana Rw1G012540 Rw1G012550 Rw2G016450 Rw4G013990 Rw4G014000 Rw4G018830 Rw4G018850 Rw4G018960 Rw4G018970 Rw6G002850 Rw6G003030 Rw6G003040 Rw6G003050 Rw6G003060 Rw6G003100 Rw6G003110 Rw6G003140 Rw6G003150 Rw7G000310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1517
AatII GACGTC 1 cut(s) 492
Acc16I TGCGCA 1 cut(s) 1493
Acc36I ACCTGC 1 cut(s) 1517
AccB1I GGYRCC 3 cut(s) 503, 1529, 1616
AccB7I CCANNNNNTGG 1 cut(s) 1322
AccIII TCCGGA 1 cut(s) 479
AciI CCGC 6 cut(s) 343, 369, 767, 1132, 1310, 1667
AclWI GGATC 6 cut(s) 64, 275, 600, 1082, 1235, 1425
AcuI CTGAAG 2 cut(s) 447, 653
AcyI GRCGYC 2 cut(s) 489, 504
AfaI GTAC 5 cut(s) 837, 853, 902, 1123, 1164
AfiI CCNNNNNNNGG 6 cut(s) 544, 622, 1322, 1336, 1370, 1540
AflIII ACRYGT 1 cut(s) 556
AgsI TTSAA 8 cut(s) 255, 572, 656, 872, 929, 1201, 1351, 1626
AjiI CACGTC 1 cut(s) 557
AjnI CCWGG 4 cut(s) 619, 741, 1150, 1321
AloI GAACNNNNNNTCC 2 cut(s) 543, 575
Alw21I GWGCWC 1 cut(s) 446
Alw26I GTCTC 3 cut(s) 450, 570, 1816
AlwI GGATC 6 cut(s) 64, 275, 600, 1082, 1235, 1425
Aor13HI TCCGGA 1 cut(s) 479
AoxI GGCC 1 cut(s) 1153
ApeKI GCWGC 4 cut(s) 165, 521, 818, 1312
ArsI GACNNNNNNTTYG 2 cut(s) 972, 1004
AseI ATTAAT 3 cut(s) 249, 1031, 1769
AspLEI GCGC 5 cut(s) 506, 521, 1494, 1584, 1781
AspS9I GGNCC 3 cut(s) 1129, 1154, 1631
AsuHPI GGTGA 3 cut(s) 86, 1352, 1731
AvaII GGWCC 2 cut(s) 1129, 1631
AxyI CCTNAGG 1 cut(s) 999
BaeGI GKGCMC 2 cut(s) 1014, 1534
BanI GGYRCC 3 cut(s) 503, 1529, 1616
BanII GRGCYC 3 cut(s) 446, 1330, 1867
BbsI GAAGAC 1 cut(s) 559
Bbv12I GWGCWC 1 cut(s) 446
BbvI GCAGC 4 cut(s) 152, 508, 805, 1324
BccI CCATC 9 cut(s) 39, 81, 253, 707, 908, 953, 1099, 1178, 1808
BciT130I CCWGG 4 cut(s) 621, 743, 1152, 1323
BciVI GTATCC 1 cut(s) 620
BcoDI GTCTC 3 cut(s) 450, 570, 1816
BfaI CTAG 1 cut(s) 1472
BfoI RGCGCY 2 cut(s) 507, 522
BfuAI ACCTGC 1 cut(s) 1517
BfuI GTATCC 1 cut(s) 620
BisI GCNGC 4 cut(s) 166, 522, 819, 1313
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 4 cut(s) 167, 523, 820, 1314
Bme1390I CCNGG 4 cut(s) 621, 743, 1152, 1323
Bme18I GGWCC 2 cut(s) 1129, 1631
BmgBI CACGTC 1 cut(s) 557
BmgT120I GGNCC 3 cut(s) 1129, 1154, 1631
BmiI GGNNCC 6 cut(s) 478, 505, 1327, 1531, 1618, 1866
BmrFI CCNGG 4 cut(s) 621, 743, 1152, 1323
BmsI GCATC 5 cut(s) 667, 1333, 1438, 1514, 1569
BpiI GAAGAC 1 cut(s) 559
BplI GAGNNNNNCTC 2 cut(s) 461, 493
BpmI CTGGAG 4 cut(s) 599, 863, 1344, 1795
Bpu1102I GCTNAGC 1 cut(s) 681
Bsa29I ATCGAT 3 cut(s) 245, 279, 675
BsaAI YACGTR 1 cut(s) 1436
BsaHI GRCGYC 2 cut(s) 489, 504
BsaJI CCNNGG 6 cut(s) 372, 538, 620, 741, 1521, 1609
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 6 cut(s) 544, 622, 1322, 1336, 1370, 1540
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 6 cut(s) 156, 846, 1375, 1499, 1633, 1634
Bse21I CCTNAGG 1 cut(s) 999
Bse3DI GCAATG 1 cut(s) 1191
BseAI TCCGGA 1 cut(s) 479
BseBI CCWGG 4 cut(s) 621, 743, 1152, 1323
BseCI ATCGAT 3 cut(s) 245, 279, 675
BseDI CCNNGG 6 cut(s) 372, 538, 620, 741, 1521, 1609
BseGI GGATG 6 cut(s) 718, 766, 810, 860, 919, 1360
BseLI CCNNNNNNNGG 6 cut(s) 544, 622, 1322, 1336, 1370, 1540
BseMI GCAATG 1 cut(s) 1191
BseMII CTCAG 6 cut(s) 80, 695, 1013, 1426, 1595, 1661
BseNI ACTGG 6 cut(s) 156, 846, 1375, 1499, 1633, 1634
BseRI GAGGAG 2 cut(s) 930, 1857
BseSI GKGCMC 2 cut(s) 1014, 1534
BseXI GCAGC 4 cut(s) 152, 508, 805, 1324
Bsh1285I CGRYCG 2 cut(s) 279, 411
BshFI GGCC 1 cut(s) 1155
BshNI GGYRCC 3 cut(s) 503, 1529, 1616
BshVI ATCGAT 3 cut(s) 245, 279, 675
BsiEI CGRYCG 2 cut(s) 279, 411
BsiHKAI GWGCWC 1 cut(s) 446
BsiSI CCGG 5 cut(s) 447, 463, 480, 507, 590
BslFI GGGAC 3 cut(s) 216, 663, 895
BslI CCNNNNNNNGG 6 cut(s) 544, 622, 1322, 1336, 1370, 1540
BsmAI GTCTC 3 cut(s) 450, 570, 1816
BsmFI GGGAC 3 cut(s) 216, 663, 895
BsmI GAATGC 1 cut(s) 168
BsnI GGCC 1 cut(s) 1155
Bsp1286I GDGCHC 5 cut(s) 446, 1014, 1330, 1534, 1867
Bsp13I TCCGGA 1 cut(s) 479
Bsp1407I TGTACA 1 cut(s) 835
Bsp143I GATC 7 cut(s) 69, 276, 280, 592, 1087, 1240, 1417
Bsp1720I GCTNAGC 1 cut(s) 681
BspACI CCGC 6 cut(s) 343, 369, 767, 1132, 1310, 1667
BspANI GGCC 1 cut(s) 1155
BspCNI CTCAG 6 cut(s) 79, 694, 1012, 1425, 1596, 1662
BspDI ATCGAT 3 cut(s) 245, 279, 675
BspEI TCCGGA 1 cut(s) 479
BspHI TCATGA 1 cut(s) 1237
BspLI GGNNCC 6 cut(s) 478, 505, 1327, 1531, 1618, 1866
BspMI ACCTGC 1 cut(s) 1517
BspPI GGATC 6 cut(s) 64, 275, 600, 1082, 1235, 1425
BspQI GCTCTTC 1 cut(s) 434
BspT107I GGYRCC 3 cut(s) 503, 1529, 1616
BsrDI GCAATG 1 cut(s) 1191
BsrFI RCCGGY 1 cut(s) 506
BsrGI TGTACA 1 cut(s) 835
BsrI ACTGG 6 cut(s) 156, 846, 1375, 1499, 1633, 1634
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 6 cut(s) 372, 538, 620, 741, 1521, 1609
BssMI GATC 7 cut(s) 69, 276, 280, 592, 1087, 1240, 1417
BssNI GRCGYC 2 cut(s) 489, 504
BssT1I CCWWGG 3 cut(s) 538, 1521, 1609
Bst2UI CCWGG 4 cut(s) 621, 743, 1152, 1323
Bst4CI ACNGT 5 cut(s) 359, 732, 750, 856, 1387
Bst6I CTCTTC 3 cut(s) 434, 831, 1459
BstACI GRCGYC 2 cut(s) 489, 504
BstAUI TGTACA 1 cut(s) 835
BstBAI YACGTR 1 cut(s) 1436
BstC8I GCNNGC 6 cut(s) 517, 1298, 1302, 1496, 1591, 1667
BstDEI CTNAG 7 cut(s) 66, 681, 909, 999, 1412, 1604, 1670
BstENI CCTNNNNNAGG 2 cut(s) 542, 1538
BstF5I GGATG 6 cut(s) 718, 766, 810, 860, 919, 1360
BstH2I RGCGCY 2 cut(s) 507, 522
BstHHI GCGC 5 cut(s) 506, 521, 1494, 1584, 1781
BstKTI GATC 7 cut(s) 72, 279, 283, 595, 1090, 1243, 1420
BstMAI GTCTC 3 cut(s) 450, 570, 1816
BstMBI GATC 7 cut(s) 69, 276, 280, 592, 1087, 1240, 1417
BstMCI CGRYCG 2 cut(s) 279, 411
BstMWI GCNNNNNNNGC 7 cut(s) 512, 521, 527, 963, 1590, 1785, 1871
BstNI CCWGG 4 cut(s) 621, 743, 1152, 1323
BstNSI RCATGY 4 cut(s) 1076, 1300, 1593, 1753
BstSCI CCNGG 4 cut(s) 619, 741, 1150, 1321
BstSLI GKGCMC 2 cut(s) 1014, 1534
BstV1I GCAGC 4 cut(s) 152, 508, 805, 1324
BstV2I GAAGAC 1 cut(s) 559
BstX2I RGATCY 2 cut(s) 69, 1417
BstYI RGATCY 2 cut(s) 69, 1417
Bsu15I ATCGAT 3 cut(s) 245, 279, 675
Bsu36I CCTNAGG 1 cut(s) 999
BsuI GTATCC 1 cut(s) 620
BsuRI GGCC 1 cut(s) 1155
BsuTUI ATCGAT 3 cut(s) 245, 279, 675
BtgZI GCGATG 1 cut(s) 972
BtrI CACGTC 1 cut(s) 557
BtsCI GGATG 6 cut(s) 718, 766, 810, 860, 919, 1360
BtsIMutI CAGTG 2 cut(s) 728, 1641
BveI ACCTGC 1 cut(s) 1517
Cac8I GCNNGC 6 cut(s) 517, 1298, 1302, 1496, 1591, 1667
CciI TCATGA 1 cut(s) 1237
CfoI GCGC 5 cut(s) 506, 521, 1494, 1584, 1781
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 3 cut(s) 1129, 1154, 1631
ClaI ATCGAT 3 cut(s) 245, 279, 675
CseI GACGC 2 cut(s) 990, 1346
Csp6I GTAC 5 cut(s) 836, 852, 901, 1122, 1163
CviAII CATG 7 cut(s) 419, 935, 1073, 1238, 1297, 1590, 1750
CviQI GTAC 5 cut(s) 836, 852, 901, 1122, 1163
DdeI CTNAG 7 cut(s) 66, 681, 909, 999, 1412, 1604, 1670
DinI GGCGCC 1 cut(s) 505
DpnI GATC 7 cut(s) 71, 278, 282, 594, 1089, 1242, 1419
DpnII GATC 7 cut(s) 69, 276, 280, 592, 1087, 1240, 1417
Eam1104I CTCTTC 3 cut(s) 434, 831, 1459
EarI CTCTTC 3 cut(s) 434, 831, 1459
EciI GGCGGA 1 cut(s) 332
Ecl136II GAGCTC 1 cut(s) 444
Eco130I CCWWGG 3 cut(s) 538, 1521, 1609
Eco24I GRGCYC 3 cut(s) 446, 1330, 1867
Eco47I GGWCC 2 cut(s) 1129, 1631
Eco53kI GAGCTC 1 cut(s) 444
Eco57I CTGAAG 2 cut(s) 447, 653
Eco81I CCTNAGG 1 cut(s) 999
EcoICRI GAGCTC 1 cut(s) 444
EcoNI CCTNNNNNAGG 2 cut(s) 542, 1538
EcoRII CCWGG 4 cut(s) 619, 741, 1150, 1321
EcoT14I CCWWGG 3 cut(s) 538, 1521, 1609
EcoT38I GRGCYC 3 cut(s) 446, 1330, 1867
EgeI GGCGCC 1 cut(s) 505
EheI GGCGCC 1 cut(s) 505
ErhI CCWWGG 3 cut(s) 538, 1521, 1609
FaeI CATG 7 cut(s) 422, 938, 1076, 1241, 1300, 1593, 1753
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 3 cut(s) 216, 663, 895
FatI CATG 7 cut(s) 418, 934, 1072, 1237, 1296, 1589, 1749
FauI CCCGC 2 cut(s) 760, 1674
Fnu4HI GCNGC 4 cut(s) 166, 522, 819, 1313
FokI GGATG 6 cut(s) 725, 773, 817, 847, 926, 1347
FriOI GRGCYC 3 cut(s) 446, 1330, 1867
Fsp4HI GCNGC 4 cut(s) 166, 522, 819, 1313
FspBI CTAG 1 cut(s) 1472
FspI TGCGCA 1 cut(s) 1493
GlaI GCGC 5 cut(s) 505, 520, 1493, 1583, 1780
GluI GCNGC 4 cut(s) 166, 522, 819, 1313
GsuI CTGGAG 4 cut(s) 599, 863, 1344, 1795
HaeII RGCGCY 2 cut(s) 507, 522
HaeIII GGCC 1 cut(s) 1155
HapII CCGG 5 cut(s) 447, 463, 480, 507, 590
HgaI GACGC 2 cut(s) 990, 1346
HhaI GCGC 5 cut(s) 506, 521, 1494, 1584, 1781
Hin1I GRCGYC 2 cut(s) 489, 504
Hin1II CATG 7 cut(s) 422, 938, 1076, 1241, 1300, 1593, 1753
Hin6I GCGC 5 cut(s) 504, 519, 1492, 1582, 1779
HinP1I GCGC 5 cut(s) 504, 519, 1492, 1582, 1779
HinfI GANTC 3 cut(s) 415, 792, 1475
HpaII CCGG 5 cut(s) 447, 463, 480, 507, 590
HphI GGTGA 3 cut(s) 86, 1352, 1731
Hpy166II GTNNAC 2 cut(s) 297, 1049
Hpy188I TCNGA 6 cut(s) 69, 427, 695, 760, 1399, 1605
Hpy188III TCNNGA 5 cut(s) 25, 480, 920, 1238, 1774
Hpy8I GTNNAC 2 cut(s) 297, 1049
Hpy99I CGWCG 2 cut(s) 351, 984
HpyAV CCTTC 7 cut(s) 107, 265, 347, 1255, 1567, 1746, 1808
HpyCH4III ACNGT 5 cut(s) 359, 732, 750, 856, 1387
HpyCH4IV ACGT 6 cut(s) 299, 349, 489, 556, 1435, 1717
HpyCH4V TGCA 7 cut(s) 4, 168, 530, 1061, 1256, 1464, 1505
HpyF10VI GCNNNNNNNGC 7 cut(s) 512, 521, 527, 963, 1590, 1785, 1871
HpyF3I CTNAG 7 cut(s) 66, 681, 909, 999, 1412, 1604, 1670
HpySE526I ACGT 6 cut(s) 299, 349, 489, 556, 1435, 1717
Hsp92I GRCGYC 2 cut(s) 489, 504
Hsp92II CATG 7 cut(s) 422, 938, 1076, 1241, 1300, 1593, 1753
HspAI GCGC 5 cut(s) 504, 519, 1492, 1582, 1779
KasI GGCGCC 1 cut(s) 503
Kpn2I TCCGGA 1 cut(s) 479
Kzo9I GATC 7 cut(s) 69, 276, 280, 592, 1087, 1240, 1417
LguI GCTCTTC 1 cut(s) 434
LmnI GCTCC 9 cut(s) 449, 482, 844, 943, 1181, 1219, 1325, 1776, 1870
Lsp1109I GCAGC 4 cut(s) 152, 508, 805, 1324
LweI GCATC 5 cut(s) 667, 1333, 1438, 1514, 1569
MaeI CTAG 1 cut(s) 1472
MaeII ACGT 6 cut(s) 299, 349, 489, 556, 1435, 1717
MaeIII GTNAC 2 cut(s) 856, 1436
MalI GATC 7 cut(s) 71, 278, 282, 594, 1089, 1242, 1419
MboI GATC 7 cut(s) 69, 276, 280, 592, 1087, 1240, 1417
MfeI CAATTG 2 cut(s) 948, 1257
MflI RGATCY 2 cut(s) 69, 1417
MhlI GDGCHC 5 cut(s) 446, 1014, 1330, 1534, 1867
MluCI AATT 9 cut(s) 238, 250, 352, 597, 885, 948, 1032, 1042, 1257
Mly113I GGCGCC 1 cut(s) 504
MmeI TCCRAC 2 cut(s) 197, 1693
MroI TCCGGA 1 cut(s) 479
MseI TTAA 8 cut(s) 249, 390, 708, 1031, 1334, 1367, 1425, 1769
MslI CAYNNNNRTG 2 cut(s) 759, 1071
MspA1I CMGCKG 3 cut(s) 165, 1134, 1669
MspI CCGG 5 cut(s) 447, 463, 480, 507, 590
MspR9I CCNGG 4 cut(s) 621, 743, 1152, 1323
MunI CAATTG 2 cut(s) 948, 1257
Mva1269I GAATGC 1 cut(s) 168
MvaI CCWGG 4 cut(s) 621, 743, 1152, 1323
MwoI GCNNNNNNNGC 7 cut(s) 512, 521, 527, 963, 1590, 1785, 1871
NarI GGCGCC 1 cut(s) 504
NdeII GATC 7 cut(s) 69, 276, 280, 592, 1087, 1240, 1417
NlaIII CATG 7 cut(s) 422, 938, 1076, 1241, 1300, 1593, 1753
NlaIV GGNNCC 6 cut(s) 478, 505, 1327, 1531, 1618, 1866
NmeAIII GCCGAG 1 cut(s) 397
NmuCI GTSAC 2 cut(s) 856, 1436
NsbI TGCGCA 1 cut(s) 1493
NspI RCATGY 4 cut(s) 1076, 1300, 1593, 1753
PaeI GCATGC 2 cut(s) 1300, 1593
PagI TCATGA 1 cut(s) 1237
PaqCI CACCTGC 1 cut(s) 1517
PciSI GCTCTTC 1 cut(s) 434
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PctI GAATGC 1 cut(s) 168
PfeI GAWTC 3 cut(s) 415, 792, 1475
PflMI CCANNNNNTGG 1 cut(s) 1322
PfoI TCCNGGA 1 cut(s) 1321
PkrI GCNGC 4 cut(s) 167, 523, 820, 1314
Ple19I CGATCG 1 cut(s) 279
PluTI GGCGCC 1 cut(s) 507
Ppu21I YACGTR 1 cut(s) 1436
PshBI ATTAAT 3 cut(s) 249, 1031, 1769
Psp124BI GAGCTC 1 cut(s) 446
Psp6I CCWGG 4 cut(s) 619, 741, 1150, 1321
PspGI CCWGG 4 cut(s) 619, 741, 1150, 1321
PspN4I GGNNCC 6 cut(s) 478, 505, 1327, 1531, 1618, 1866
PspPI GGNCC 3 cut(s) 1129, 1154, 1631
PsuI RGATCY 2 cut(s) 69, 1417
PvuI CGATCG 1 cut(s) 279
PvuII CAGCTG 1 cut(s) 165
RsaI GTAC 5 cut(s) 837, 853, 902, 1123, 1164
RsaNI GTAC 5 cut(s) 836, 852, 901, 1122, 1163
RseI CAYNNNNRTG 2 cut(s) 759, 1071
SacI GAGCTC 1 cut(s) 446
SapI GCTCTTC 1 cut(s) 434
SaqAI TTAA 8 cut(s) 249, 390, 708, 1031, 1334, 1367, 1425, 1769
SatI GCNGC 4 cut(s) 166, 522, 819, 1313
Sau3AI GATC 7 cut(s) 69, 276, 280, 592, 1087, 1240, 1417
Sau96I GGNCC 3 cut(s) 1129, 1154, 1631
ScrFI CCNGG 4 cut(s) 621, 743, 1152, 1323
SduI GDGCHC 5 cut(s) 446, 1014, 1330, 1534, 1867
SfaNI GCATC 5 cut(s) 667, 1333, 1438, 1514, 1569
SfoI GGCGCC 1 cut(s) 505
SgrAI CRCCGGYG 1 cut(s) 506
SinI GGWCC 2 cut(s) 1129, 1631
SmiMI CAYNNNNRTG 2 cut(s) 759, 1071
SphI GCATGC 2 cut(s) 1300, 1593
Sse9I AATT 9 cut(s) 238, 250, 352, 597, 885, 948, 1032, 1042, 1257
SsiI CCGC 6 cut(s) 343, 369, 767, 1132, 1310, 1667
SspDI GGCGCC 1 cut(s) 503
SspI AATATT 1 cut(s) 1794
SspMI CTAG 1 cut(s) 1472
SstI GAGCTC 1 cut(s) 446
StyD4I CCNGG 4 cut(s) 619, 741, 1150, 1321
StyI CCWWGG 3 cut(s) 538, 1521, 1609
TaaI ACNGT 5 cut(s) 359, 732, 750, 856, 1387
TaiI ACGT 6 cut(s) 302, 352, 492, 559, 1438, 1720
TaqI TCGA 4 cut(s) 245, 279, 595, 675
TaqII GACCGA 1 cut(s) 425
TasI AATT 9 cut(s) 238, 250, 352, 597, 885, 948, 1032, 1042, 1257
TatI WGTACW 3 cut(s) 835, 900, 1121
TfiI GAWTC 3 cut(s) 415, 792, 1475
Tru1I TTAA 8 cut(s) 249, 390, 708, 1031, 1334, 1367, 1425, 1769
Tru9I TTAA 8 cut(s) 249, 390, 708, 1031, 1334, 1367, 1425, 1769
TscAI CASTG 2 cut(s) 735, 1641
TseFI GTSAC 2 cut(s) 856, 1436
TseI GCWGC 4 cut(s) 165, 521, 818, 1312
Tsp45I GTSAC 2 cut(s) 856, 1436
TspDTI ATGAA 7 cut(s) 213, 231, 284, 784, 1226, 1237, 1446
TspGWI ACGGA 2 cut(s) 273, 468
TspRI CASTG 2 cut(s) 735, 1641
Van91I CCANNNNNTGG 1 cut(s) 1322
VpaK11BI GGWCC 2 cut(s) 1129, 1631
VspI ATTAAT 3 cut(s) 249, 1031, 1769
XagI CCTNNNNNAGG 2 cut(s) 542, 1538
XceI RCATGY 4 cut(s) 1076, 1300, 1593, 1753
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
XspI CTAG 1 cut(s) 1472
ZraI GACGTC 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.