Peptidase, trypsin-like serine and cysteine proteases
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 288 |
| AccB1I | GGYRCC | 1 cut(s) | 179 |
| AccI | GTMKAC | 3 cut(s) | 516, 705, 993 |
| AccII | CGCG | 1 cut(s) | 780 |
| AciI | CCGC | 5 cut(s) | 5, 160, 780, 783, 980 |
| AclWI | GGATC | 1 cut(s) | 64 |
| AcoI | YGGCCR | 1 cut(s) | 874 |
| AcsI | RAATTY | 1 cut(s) | 125 |
| AcuI | CTGAAG | 2 cut(s) | 42, 358 |
| AcyI | GRCGYC | 4 cut(s) | 180, 218, 285, 990 |
| AfaI | GTAC | 4 cut(s) | 443, 570, 881, 910 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 72, 205, 392, 486 |
| AgsI | TTSAA | 2 cut(s) | 145, 616 |
| AjiI | CACGTC | 2 cut(s) | 205, 409 |
| AjnI | CCWGG | 3 cut(s) | 391, 871, 1003 |
| AluBI | AGCT | 8 cut(s) | 152, 190, 250, 551, 581, 834, 847, 906 |
| AluI | AGCT | 8 cut(s) | 152, 190, 250, 551, 581, 834, 847, 906 |
| Alw21I | GWGCWC | 1 cut(s) | 553 |
| Alw26I | GTCTC | 3 cut(s) | 91, 498, 748 |
| AlwI | GGATC | 1 cut(s) | 64 |
| Ama87I | CYCGRG | 1 cut(s) | 263 |
| AoxI | GGCC | 2 cut(s) | 633, 874 |
| ApeKI | GCWGC | 2 cut(s) | 52, 149 |
| ApoI | RAATTY | 1 cut(s) | 125 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 110, 142 |
| AseI | ATTAAT | 1 cut(s) | 1062 |
| AspLEI | GCGC | 2 cut(s) | 182, 780 |
| AspS9I | GGNCC | 2 cut(s) | 633, 869 |
| AsuC2I | CCSGG | 1 cut(s) | 359 |
| AsuHPI | GGTGA | 3 cut(s) | 46, 70, 280 |
| AvaI | CYCGRG | 1 cut(s) | 263 |
| AvaII | GGWCC | 1 cut(s) | 869 |
| BanI | GGYRCC | 1 cut(s) | 179 |
| BanII | GRGCYC | 2 cut(s) | 302, 553 |
| BauI | CACGAG | 1 cut(s) | 835 |
| BbsI | GAAGAC | 1 cut(s) | 465 |
| Bbv12I | GWGCWC | 1 cut(s) | 553 |
| BbvCI | CCTCAGC | 1 cut(s) | 186 |
| BbvI | GCAGC | 2 cut(s) | 39, 161 |
| BccI | CCATC | 3 cut(s) | 90, 203, 494 |
| BceAI | ACGGC | 2 cut(s) | 231, 264 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 153, 187 |
| BciT130I | CCWGG | 3 cut(s) | 393, 873, 1005 |
| BciVI | GTATCC | 1 cut(s) | 504 |
| BclI | TGATCA | 1 cut(s) | 964 |
| BcnI | CCSGG | 1 cut(s) | 359 |
| BcoDI | GTCTC | 3 cut(s) | 91, 498, 748 |
| BfaI | CTAG | 3 cut(s) | 200, 323, 1026 |
| BfmI | CTRYAG | 1 cut(s) | 544 |
| BfoI | RGCGCY | 1 cut(s) | 183 |
| BfuI | GTATCC | 1 cut(s) | 504 |
| BisI | GCNGC | 5 cut(s) | 53, 150, 161, 781, 981 |
| BlpI | GCTNAGC | 2 cut(s) | 251, 577 |
| BlsI | GCNGC | 5 cut(s) | 54, 151, 162, 782, 982 |
| Bme1390I | CCNGG | 4 cut(s) | 359, 393, 873, 1005 |
| Bme18I | GGWCC | 1 cut(s) | 869 |
| BmeT110I | CYCGRG | 1 cut(s) | 263 |
| BmgBI | CACGTC | 2 cut(s) | 205, 409 |
| BmgT120I | GGNCC | 2 cut(s) | 633, 869 |
| BmiI | GGNNCC | 4 cut(s) | 181, 301, 356, 1002 |
| BmrFI | CCNGG | 4 cut(s) | 359, 393, 873, 1005 |
| BmrI | ACTGGG | 2 cut(s) | 305, 814 |
| BmsI | GCATC | 3 cut(s) | 140, 743, 1017 |
| BmuI | ACTGGG | 2 cut(s) | 305, 814 |
| BpiI | GAAGAC | 1 cut(s) | 465 |
| Bpu10I | CCTNAGC | 2 cut(s) | 186, 600 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 251, 577 |
| BpuMI | CCSGG | 1 cut(s) | 359 |
| BsaHI | GRCGYC | 4 cut(s) | 180, 218, 285, 990 |
| BsaI | GGTCTC | 1 cut(s) | 748 |
| BsaJI | CCNNGG | 1 cut(s) | 391 |
| BsaWI | WCCGGW | 1 cut(s) | 72 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 221, 251 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 72, 205, 392, 486 |
| Bse118I | RCCGGY | 3 cut(s) | 176, 866, 876 |
| Bse1I | ACTGG | 3 cut(s) | 300, 809, 950 |
| Bse3DI | GCAATG | 1 cut(s) | 651 |
| BseBI | CCWGG | 3 cut(s) | 393, 873, 1005 |
| BseDI | CCNNGG | 1 cut(s) | 391 |
| BseGI | GGATG | 2 cut(s) | 214, 758 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 72, 205, 392, 486 |
| BseMI | GCAATG | 1 cut(s) | 651 |
| BseMII | CTCAG | 4 cut(s) | 200, 591, 663, 738 |
| BseNI | ACTGG | 3 cut(s) | 300, 809, 950 |
| BseXI | GCAGC | 2 cut(s) | 39, 161 |
| BsgI | GTGCAG | 1 cut(s) | 157 |
| Bsh1236I | CGCG | 1 cut(s) | 780 |
| BshFI | GGCC | 2 cut(s) | 635, 876 |
| BshNI | GGYRCC | 1 cut(s) | 179 |
| BsiHKAI | GWGCWC | 1 cut(s) | 553 |
| BsiHKCI | CYCGRG | 1 cut(s) | 263 |
| BsiSI | CCGG | 5 cut(s) | 73, 177, 359, 867, 877 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 72, 205, 392, 486 |
| BsmAI | GTCTC | 3 cut(s) | 91, 498, 748 |
| BsmBI | CGTCTC | 1 cut(s) | 91 |
| BsnI | GGCC | 2 cut(s) | 635, 876 |
| Bso31I | GGTCTC | 1 cut(s) | 748 |
| BsoBI | CYCGRG | 1 cut(s) | 263 |
| Bsp1286I | GDGCHC | 2 cut(s) | 302, 553 |
| Bsp1407I | TGTACA | 1 cut(s) | 908 |
| Bsp143I | GATC | 2 cut(s) | 69, 964 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 251, 577 |
| BspACI | CCGC | 5 cut(s) | 5, 160, 780, 783, 980 |
| BspANI | GGCC | 2 cut(s) | 635, 876 |
| BspCNI | CTCAG | 4 cut(s) | 199, 590, 664, 739 |
| BspFNI | CGCG | 1 cut(s) | 780 |
| BspHI | TCATGA | 2 cut(s) | 787, 810 |
| BspLI | GGNNCC | 4 cut(s) | 181, 301, 356, 1002 |
| BspPI | GGATC | 1 cut(s) | 64 |
| BspT107I | GGYRCC | 1 cut(s) | 179 |
| BspTNI | GGTCTC | 1 cut(s) | 748 |
| BsrDI | GCAATG | 1 cut(s) | 651 |
| BsrFI | RCCGGY | 3 cut(s) | 176, 866, 876 |
| BsrGI | TGTACA | 1 cut(s) | 908 |
| BsrI | ACTGG | 3 cut(s) | 300, 809, 950 |
| BssAI | RCCGGY | 3 cut(s) | 176, 866, 876 |
| BssECI | CCNNGG | 1 cut(s) | 391 |
| BssMI | GATC | 2 cut(s) | 69, 964 |
| BssNI | GRCGYC | 4 cut(s) | 180, 218, 285, 990 |
| BssSI | CACGAG | 1 cut(s) | 835 |
| Bst2BI | CACGAG | 1 cut(s) | 835 |
| Bst2UI | CCWGG | 3 cut(s) | 393, 873, 1005 |
| Bst4CI | ACNGT | 5 cut(s) | 34, 311, 568, 913, 1000 |
| BstACI | GRCGYC | 4 cut(s) | 180, 218, 285, 990 |
| BstAUI | TGTACA | 1 cut(s) | 908 |
| BstDEI | CTNAG | 8 cut(s) | 186, 251, 531, 577, 600, 672, 747, 843 |
| BstF5I | GGATG | 2 cut(s) | 214, 758 |
| BstFNI | CGCG | 1 cut(s) | 780 |
| BstH2I | RGCGCY | 1 cut(s) | 183 |
| BstHHI | GCGC | 2 cut(s) | 182, 780 |
| BstKTI | GATC | 2 cut(s) | 72, 967 |
| BstMAI | GTCTC | 3 cut(s) | 91, 498, 748 |
| BstMBI | GATC | 2 cut(s) | 69, 964 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 831, 1026 |
| BstNI | CCWGG | 3 cut(s) | 393, 873, 1005 |
| BstSCI | CCNGG | 4 cut(s) | 357, 391, 871, 1003 |
| BstSFI | CTRYAG | 1 cut(s) | 544 |
| BstUI | CGCG | 1 cut(s) | 780 |
| BstV1I | GCAGC | 2 cut(s) | 39, 161 |
| BstV2I | GAAGAC | 1 cut(s) | 465 |
| BsuI | GTATCC | 1 cut(s) | 504 |
| BsuRI | GGCC | 2 cut(s) | 635, 876 |
| BtrI | CACGTC | 2 cut(s) | 205, 409 |
| BtsCI | GGATG | 2 cut(s) | 214, 758 |
| BtsIMutI | CAGTG | 1 cut(s) | 943 |
| CciI | TCATGA | 2 cut(s) | 787, 810 |
| CfoI | GCGC | 2 cut(s) | 182, 780 |
| Cfr10I | RCCGGY | 3 cut(s) | 176, 866, 876 |
| Cfr13I | GGNCC | 2 cut(s) | 633, 869 |
| CseI | GACGC | 2 cut(s) | 207, 979 |
| Csp6I | GTAC | 4 cut(s) | 442, 569, 880, 909 |
| CviAII | CATG | 3 cut(s) | 788, 811, 1030 |
| CviQI | GTAC | 4 cut(s) | 442, 569, 880, 909 |
| DdeI | CTNAG | 8 cut(s) | 186, 251, 531, 577, 600, 672, 747, 843 |
| DinI | GGCGCC | 1 cut(s) | 181 |
| DpnI | GATC | 2 cut(s) | 71, 966 |
| DpnII | GATC | 2 cut(s) | 69, 964 |
| EaeI | YGGCCR | 1 cut(s) | 874 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Ecl136II | GAGCTC | 1 cut(s) | 551 |
| Eco24I | GRGCYC | 2 cut(s) | 302, 553 |
| Eco31I | GGTCTC | 1 cut(s) | 748 |
| Eco47I | GGWCC | 1 cut(s) | 869 |
| Eco53kI | GAGCTC | 1 cut(s) | 551 |
| Eco57I | CTGAAG | 2 cut(s) | 42, 358 |
| Eco88I | CYCGRG | 1 cut(s) | 263 |
| EcoICRI | GAGCTC | 1 cut(s) | 551 |
| EcoRII | CCWGG | 3 cut(s) | 391, 871, 1003 |
| EcoT38I | GRGCYC | 2 cut(s) | 302, 553 |
| EgeI | GGCGCC | 1 cut(s) | 181 |
| EheI | GGCGCC | 1 cut(s) | 181 |
| Esp3I | CGTCTC | 1 cut(s) | 91 |
| FaeI | CATG | 3 cut(s) | 791, 814, 1033 |
| FatI | CATG | 3 cut(s) | 787, 810, 1029 |
| FauNDI | CATATG | 2 cut(s) | 484, 1039 |
| FbaI | TGATCA | 1 cut(s) | 964 |
| FblI | GTMKAC | 3 cut(s) | 516, 705, 993 |
| Fnu4HI | GCNGC | 5 cut(s) | 53, 150, 161, 781, 981 |
| FokI | GGATG | 2 cut(s) | 221, 765 |
| FriOI | GRGCYC | 2 cut(s) | 302, 553 |
| Fsp4HI | GCNGC | 5 cut(s) | 53, 150, 161, 781, 981 |
| FspBI | CTAG | 3 cut(s) | 200, 323, 1026 |
| GlaI | GCGC | 2 cut(s) | 181, 779 |
| GluI | GCNGC | 5 cut(s) | 53, 150, 161, 781, 981 |
| HaeII | RGCGCY | 1 cut(s) | 183 |
| HaeIII | GGCC | 2 cut(s) | 635, 876 |
| HapII | CCGG | 5 cut(s) | 73, 177, 359, 867, 877 |
| HgaI | GACGC | 2 cut(s) | 207, 979 |
| HhaI | GCGC | 2 cut(s) | 182, 780 |
| Hin1I | GRCGYC | 4 cut(s) | 180, 218, 285, 990 |
| Hin1II | CATG | 3 cut(s) | 791, 814, 1033 |
| Hin6I | GCGC | 2 cut(s) | 180, 778 |
| HinP1I | GCGC | 2 cut(s) | 180, 778 |
| HinfI | GANTC | 7 cut(s) | 194, 332, 369, 506, 607, 791, 1033 |
| HpaII | CCGG | 5 cut(s) | 73, 177, 359, 867, 877 |
| HphI | GGTGA | 3 cut(s) | 46, 70, 280 |
| Hpy166II | GTNNAC | 5 cut(s) | 78, 517, 706, 840, 994 |
| Hpy188I | TCNGA | 4 cut(s) | 118, 245, 748, 1059 |
| Hpy188III | TCNNGA | 8 cut(s) | 88, 263, 352, 401, 497, 682, 788, 811 |
| Hpy8I | GTNNAC | 5 cut(s) | 78, 517, 706, 840, 994 |
| Hpy99I | CGWCG | 2 cut(s) | 223, 273 |
| HpyAV | CCTTC | 2 cut(s) | 178, 243 |
| HpyCH4III | ACNGT | 5 cut(s) | 34, 311, 568, 913, 1000 |
| HpyCH4IV | ACGT | 4 cut(s) | 204, 285, 408, 425 |
| HpyCH4V | TGCA | 3 cut(s) | 174, 306, 917 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 831, 1026 |
| HpyF3I | CTNAG | 8 cut(s) | 186, 251, 531, 577, 600, 672, 747, 843 |
| HpySE526I | ACGT | 4 cut(s) | 204, 285, 408, 425 |
| Hsp92I | GRCGYC | 4 cut(s) | 180, 218, 285, 990 |
| Hsp92II | CATG | 3 cut(s) | 791, 814, 1033 |
| HspAI | GCGC | 2 cut(s) | 180, 778 |
| KasI | GGCGCC | 1 cut(s) | 179 |
| Ksp22I | TGATCA | 1 cut(s) | 964 |
| Kzo9I | GATC | 2 cut(s) | 69, 964 |
| LmnI | GCTCC | 1 cut(s) | 305 |
| Lsp1109I | GCAGC | 2 cut(s) | 39, 161 |
| LweI | GCATC | 3 cut(s) | 140, 743, 1017 |
| MaeI | CTAG | 3 cut(s) | 200, 323, 1026 |
| MaeII | ACGT | 4 cut(s) | 204, 285, 408, 425 |
| MaeIII | GTNAC | 4 cut(s) | 28, 281, 286, 338 |
| MalI | GATC | 2 cut(s) | 71, 966 |
| MboI | GATC | 2 cut(s) | 69, 964 |
| MboII | GAAGA | 3 cut(s) | 35, 364, 470 |
| MhlI | GDGCHC | 2 cut(s) | 302, 553 |
| MluCI | AATT | 2 cut(s) | 125, 140 |
| Mly113I | GGCGCC | 1 cut(s) | 180 |
| MlyI | GAGTC | 2 cut(s) | 500, 785 |
| MmeI | TCCRAC | 1 cut(s) | 141 |
| MseI | TTAA | 3 cut(s) | 852, 1014, 1062 |
| MspA1I | CMGCKG | 1 cut(s) | 160 |
| MspI | CCGG | 5 cut(s) | 73, 177, 359, 867, 877 |
| MspR9I | CCNGG | 4 cut(s) | 359, 393, 873, 1005 |
| MvaI | CCWGG | 3 cut(s) | 393, 873, 1005 |
| MvnI | CGCG | 1 cut(s) | 780 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 831, 1026 |
| NarI | GGCGCC | 1 cut(s) | 180 |
| NciI | CCSGG | 1 cut(s) | 359 |
| NdeI | CATATG | 2 cut(s) | 484, 1039 |
| NdeII | GATC | 2 cut(s) | 69, 964 |
| NlaIII | CATG | 3 cut(s) | 791, 814, 1033 |
| NlaIV | GGNNCC | 4 cut(s) | 181, 301, 356, 1002 |
| NmuCI | GTSAC | 3 cut(s) | 28, 281, 286 |
| PaeR7I | CTCGAG | 1 cut(s) | 263 |
| PagI | TCATGA | 2 cut(s) | 787, 810 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 160, 277 |
| PfeI | GAWTC | 5 cut(s) | 194, 332, 369, 607, 1033 |
| PflFI | GACNNNGTC | 1 cut(s) | 407 |
| PkrI | GCNGC | 5 cut(s) | 54, 151, 162, 782, 982 |
| PleI | GAGTC | 2 cut(s) | 500, 785 |
| PluTI | GGCGCC | 1 cut(s) | 183 |
| PpsI | GAGTC | 2 cut(s) | 500, 785 |
| PshBI | ATTAAT | 1 cut(s) | 1062 |
| Psp124BI | GAGCTC | 1 cut(s) | 553 |
| Psp6I | CCWGG | 3 cut(s) | 391, 871, 1003 |
| PspGI | CCWGG | 3 cut(s) | 391, 871, 1003 |
| PspN4I | GGNNCC | 4 cut(s) | 181, 301, 356, 1002 |
| PspPI | GGNCC | 2 cut(s) | 633, 869 |
| PsyI | GACNNNGTC | 1 cut(s) | 407 |
| RsaI | GTAC | 4 cut(s) | 443, 570, 881, 910 |
| RsaNI | GTAC | 4 cut(s) | 442, 569, 880, 909 |
| SacI | GAGCTC | 1 cut(s) | 553 |
| SaqAI | TTAA | 3 cut(s) | 852, 1014, 1062 |
| SatI | GCNGC | 5 cut(s) | 53, 150, 161, 781, 981 |
| Sau3AI | GATC | 2 cut(s) | 69, 964 |
| Sau96I | GGNCC | 2 cut(s) | 633, 869 |
| SchI | GAGTC | 2 cut(s) | 500, 785 |
| ScrFI | CCNGG | 4 cut(s) | 359, 393, 873, 1005 |
| SduI | GDGCHC | 2 cut(s) | 302, 553 |
| SfaNI | GCATC | 3 cut(s) | 140, 743, 1017 |
| SfcI | CTRYAG | 1 cut(s) | 544 |
| SfoI | GGCGCC | 1 cut(s) | 181 |
| Sfr274I | CTCGAG | 1 cut(s) | 263 |
| SgrAI | CRCCGGYG | 1 cut(s) | 176 |
| SinI | GGWCC | 1 cut(s) | 869 |
| SlaI | CTCGAG | 1 cut(s) | 263 |
| SmlI | CTYRAG | 1 cut(s) | 263 |
| SmoI | CTYRAG | 1 cut(s) | 263 |
| Sse9I | AATT | 2 cut(s) | 125, 140 |
| SsiI | CCGC | 5 cut(s) | 5, 160, 780, 783, 980 |
| SspDI | GGCGCC | 1 cut(s) | 179 |
| SspI | AATATT | 1 cut(s) | 40 |
| SspMI | CTAG | 3 cut(s) | 200, 323, 1026 |
| SstI | GAGCTC | 1 cut(s) | 553 |
| StyD4I | CCNGG | 4 cut(s) | 357, 391, 871, 1003 |
| TaaI | ACNGT | 5 cut(s) | 34, 311, 568, 913, 1000 |
| TaiI | ACGT | 4 cut(s) | 207, 288, 411, 428 |
| TaqI | TCGA | 5 cut(s) | 89, 192, 264, 641, 660 |
| TasI | AATT | 2 cut(s) | 125, 140 |
| TatI | WGTACW | 1 cut(s) | 908 |
| TauI | GCSGC | 3 cut(s) | 163, 783, 983 |
| TfiI | GAWTC | 5 cut(s) | 194, 332, 369, 607, 1033 |
| Tru1I | TTAA | 3 cut(s) | 852, 1014, 1062 |
| Tru9I | TTAA | 3 cut(s) | 852, 1014, 1062 |
| TscAI | CASTG | 1 cut(s) | 950 |
| TseFI | GTSAC | 3 cut(s) | 28, 281, 286 |
| TseI | GCWGC | 2 cut(s) | 52, 149 |
| Tsp45I | GTSAC | 3 cut(s) | 28, 281, 286 |
| TspDTI | ATGAA | 3 cut(s) | 94, 617, 660 |
| TspGWI | ACGGA | 3 cut(s) | 73, 229, 897 |
| TspRI | CASTG | 1 cut(s) | 950 |
| Tth111I | GACNNNGTC | 1 cut(s) | 407 |
| VpaK11BI | GGWCC | 1 cut(s) | 869 |
| VspI | ATTAAT | 1 cut(s) | 1062 |
| XapI | RAATTY | 1 cut(s) | 125 |
| XhoI | CTCGAG | 1 cut(s) | 263 |
| XmiI | GTMKAC | 3 cut(s) | 516, 705, 993 |
| XspI | CTAG | 3 cut(s) | 200, 323, 1026 |
| ZraI | GACGTC | 1 cut(s) | 286 |