Peptidase, trypsin-like serine and cysteine proteases
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 312 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 910 |
| AccI | GTMKAC | 2 cut(s) | 219, 652 |
| AciI | CCGC | 3 cut(s) | 5, 132, 160 |
| AcsI | RAATTY | 2 cut(s) | 691, 847 |
| AcuI | CTGAAG | 1 cut(s) | 42 |
| AcvI | CACGTG | 1 cut(s) | 305 |
| AcyI | GRCGYC | 2 cut(s) | 236, 309 |
| AfaI | GTAC | 2 cut(s) | 871, 953 |
| AfeI | AGCGCT | 1 cut(s) | 752 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 392, 419, 507, 885, 910 |
| AflIII | ACRYGT | 1 cut(s) | 990 |
| AgsI | TTSAA | 3 cut(s) | 403, 632, 962 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 648, 799 |
| AjiI | CACGTC | 1 cut(s) | 223 |
| AjnI | CCWGG | 2 cut(s) | 418, 903 |
| AluBI | AGCT | 7 cut(s) | 152, 442, 596, 742, 808, 867, 969 |
| AluI | AGCT | 7 cut(s) | 152, 442, 596, 742, 808, 867, 969 |
| Alw21I | GWGCWC | 2 cut(s) | 444, 598 |
| Alw26I | GTCTC | 2 cut(s) | 91, 557 |
| Ama87I | CYCGRG | 1 cut(s) | 281 |
| Aor51HI | AGCGCT | 1 cut(s) | 752 |
| AoxI | GGCC | 1 cut(s) | 295 |
| ApaI | GGGCCC | 1 cut(s) | 299 |
| ApeKI | GCWGC | 5 cut(s) | 52, 149, 497, 966, 981 |
| ApoI | RAATTY | 2 cut(s) | 691, 847 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 110, 142 |
| AseI | ATTAAT | 1 cut(s) | 1023 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 581 |
| AspLEI | GCGC | 1 cut(s) | 753 |
| AspS9I | GGNCC | 2 cut(s) | 295, 296 |
| AsuHPI | GGTGA | 1 cut(s) | 70 |
| AsuII | TTCGAA | 1 cut(s) | 144 |
| AvaI | CYCGRG | 1 cut(s) | 281 |
| BaeGI | GKGCMC | 1 cut(s) | 299 |
| BanII | GRGCYC | 4 cut(s) | 299, 326, 444, 598 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 996, 1028 |
| BbrPI | CACGTG | 1 cut(s) | 305 |
| BbsI | GAAGAC | 2 cut(s) | 543, 549 |
| Bbv12I | GWGCWC | 2 cut(s) | 444, 598 |
| BbvI | GCAGC | 5 cut(s) | 39, 161, 509, 978, 993 |
| BccI | CCATC | 1 cut(s) | 90 |
| BceAI | ACGGC | 1 cut(s) | 249 |
| BciT130I | CCWGG | 2 cut(s) | 420, 905 |
| BclI | TGATCA | 1 cut(s) | 922 |
| BcoDI | GTCTC | 2 cut(s) | 91, 557 |
| BfaI | CTAG | 1 cut(s) | 170 |
| BfmI | CTRYAG | 2 cut(s) | 216, 484 |
| BfoI | RGCGCY | 1 cut(s) | 754 |
| BisI | GCNGC | 6 cut(s) | 53, 132, 150, 498, 967, 982 |
| BlsI | GCNGC | 6 cut(s) | 54, 133, 151, 499, 968, 983 |
| Bme1390I | CCNGG | 2 cut(s) | 420, 905 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 648, 799 |
| BmeT110I | CYCGRG | 1 cut(s) | 281 |
| BmgBI | CACGTC | 1 cut(s) | 223 |
| BmgT120I | GGNCC | 2 cut(s) | 295, 296 |
| BmiI | GGNNCC | 5 cut(s) | 296, 297, 325, 383, 417 |
| BmrFI | CCNGG | 2 cut(s) | 420, 905 |
| BmrI | ACTGGG | 1 cut(s) | 329 |
| BmsI | GCATC | 3 cut(s) | 143, 346, 710 |
| BmuI | ACTGGG | 1 cut(s) | 329 |
| BpiI | GAAGAC | 2 cut(s) | 543, 549 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 262, 294, 283, 315 |
| BpmI | CTGGAG | 1 cut(s) | 574 |
| Bpu10I | CCTNAGC | 1 cut(s) | 269 |
| Bpu14I | TTCGAA | 1 cut(s) | 144 |
| BsaAI | YACGTR | 2 cut(s) | 305, 951 |
| BsaHI | GRCGYC | 2 cut(s) | 236, 309 |
| BsaJI | CCNNGG | 1 cut(s) | 823 |
| BsaWI | WCCGGW | 1 cut(s) | 392 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 392, 419, 507, 885, 910 |
| Bse1I | ACTGG | 1 cut(s) | 324 |
| Bse3DI | GCAATG | 1 cut(s) | 337 |
| BseBI | CCWGG | 2 cut(s) | 420, 905 |
| BseDI | CCNNGG | 1 cut(s) | 823 |
| BseGI | GGATG | 2 cut(s) | 725, 796 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 392, 419, 507, 885, 910 |
| BseMI | GCAATG | 1 cut(s) | 337 |
| BseMII | CTCAG | 3 cut(s) | 260, 705, 734 |
| BseNI | ACTGG | 1 cut(s) | 324 |
| BseRI | GAGGAG | 1 cut(s) | 577 |
| BseSI | GKGCMC | 1 cut(s) | 299 |
| BseXI | GCAGC | 5 cut(s) | 39, 161, 509, 978, 993 |
| BseYI | CCCAGC | 1 cut(s) | 385 |
| BsgI | GTGCAG | 1 cut(s) | 754 |
| BshFI | GGCC | 1 cut(s) | 297 |
| BsiHKAI | GWGCWC | 2 cut(s) | 444, 598 |
| BsiHKCI | CYCGRG | 1 cut(s) | 281 |
| BsiSI | CCGG | 2 cut(s) | 393, 413 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 392, 419, 507, 885, 910 |
| BsmAI | GTCTC | 2 cut(s) | 91, 557 |
| BsmBI | CGTCTC | 1 cut(s) | 91 |
| BsnI | GGCC | 1 cut(s) | 297 |
| BsoBI | CYCGRG | 1 cut(s) | 281 |
| Bsp119I | TTCGAA | 1 cut(s) | 144 |
| Bsp120I | GGGCCC | 1 cut(s) | 295 |
| Bsp1286I | GDGCHC | 4 cut(s) | 299, 326, 444, 598 |
| Bsp1407I | TGTACA | 1 cut(s) | 869 |
| Bsp143I | GATC | 1 cut(s) | 922 |
| Bsp19I | CCATGG | 1 cut(s) | 823 |
| BspACI | CCGC | 3 cut(s) | 5, 132, 160 |
| BspANI | GGCC | 1 cut(s) | 297 |
| BspCNI | CTCAG | 3 cut(s) | 261, 706, 735 |
| BspHI | TCATGA | 1 cut(s) | 771 |
| BspLI | GGNNCC | 5 cut(s) | 296, 297, 325, 383, 417 |
| BspT104I | TTCGAA | 1 cut(s) | 144 |
| BsrDI | GCAATG | 1 cut(s) | 337 |
| BsrGI | TGTACA | 1 cut(s) | 869 |
| BsrI | ACTGG | 1 cut(s) | 324 |
| BssECI | CCNNGG | 1 cut(s) | 823 |
| BssMI | GATC | 1 cut(s) | 922 |
| BssNI | GRCGYC | 2 cut(s) | 236, 309 |
| BssT1I | CCWWGG | 1 cut(s) | 823 |
| Bst2UI | CCWGG | 2 cut(s) | 420, 905 |
| Bst4CI | ACNGT | 3 cut(s) | 434, 602, 874 |
| Bst6I | CTCTTC | 3 cut(s) | 564, 627, 999 |
| BstACI | GRCGYC | 2 cut(s) | 236, 309 |
| BstAUI | TGTACA | 1 cut(s) | 869 |
| BstBAI | YACGTR | 2 cut(s) | 305, 951 |
| BstBI | TTCGAA | 1 cut(s) | 144 |
| BstC8I | GCNNGC | 1 cut(s) | 971 |
| BstDEI | CTNAG | 4 cut(s) | 269, 714, 743, 804 |
| BstDSI | CCRYGG | 1 cut(s) | 823 |
| BstF5I | GGATG | 2 cut(s) | 725, 796 |
| BstH2I | RGCGCY | 1 cut(s) | 754 |
| BstHHI | GCGC | 1 cut(s) | 753 |
| BstKTI | GATC | 1 cut(s) | 925 |
| BstMAI | GTCTC | 2 cut(s) | 91, 557 |
| BstMBI | GATC | 1 cut(s) | 922 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 233, 356, 748 |
| BstNI | CCWGG | 2 cut(s) | 420, 905 |
| BstNSI | RCATGY | 1 cut(s) | 994 |
| BstSCI | CCNGG | 2 cut(s) | 418, 903 |
| BstSFI | CTRYAG | 2 cut(s) | 216, 484 |
| BstSLI | GKGCMC | 1 cut(s) | 299 |
| BstV1I | GCAGC | 5 cut(s) | 39, 161, 509, 978, 993 |
| BstV2I | GAAGAC | 2 cut(s) | 543, 549 |
| BsuRI | GGCC | 1 cut(s) | 297 |
| BtgI | CCRYGG | 1 cut(s) | 823 |
| BtgZI | GCGATG | 1 cut(s) | 120 |
| BtrI | CACGTC | 1 cut(s) | 223 |
| BtsCI | GGATG | 2 cut(s) | 725, 796 |
| Cac8I | GCNNGC | 1 cut(s) | 971 |
| CciI | TCATGA | 1 cut(s) | 771 |
| CfoI | GCGC | 1 cut(s) | 753 |
| Cfr13I | GGNCC | 2 cut(s) | 295, 296 |
| CseI | GACGC | 2 cut(s) | 225, 638 |
| Csp6I | GTAC | 2 cut(s) | 870, 952 |
| CviAII | CATG | 6 cut(s) | 445, 452, 772, 798, 824, 991 |
| CviQI | GTAC | 2 cut(s) | 870, 952 |
| DdeI | CTNAG | 4 cut(s) | 269, 714, 743, 804 |
| DpnI | GATC | 1 cut(s) | 924 |
| DpnII | GATC | 1 cut(s) | 922 |
| DriI | GACNNNNNGTC | 2 cut(s) | 648, 799 |
| Eam1104I | CTCTTC | 3 cut(s) | 564, 627, 999 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 648, 799 |
| EarI | CTCTTC | 3 cut(s) | 564, 627, 999 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Ecl136II | GAGCTC | 2 cut(s) | 442, 596 |
| Eco130I | CCWWGG | 1 cut(s) | 823 |
| Eco24I | GRGCYC | 4 cut(s) | 299, 326, 444, 598 |
| Eco47III | AGCGCT | 1 cut(s) | 752 |
| Eco53kI | GAGCTC | 2 cut(s) | 442, 596 |
| Eco57I | CTGAAG | 1 cut(s) | 42 |
| Eco72I | CACGTG | 1 cut(s) | 305 |
| Eco88I | CYCGRG | 1 cut(s) | 281 |
| EcoICRI | GAGCTC | 2 cut(s) | 442, 596 |
| EcoO109I | RGGNCCY | 2 cut(s) | 295, 296 |
| EcoRII | CCWGG | 2 cut(s) | 418, 903 |
| EcoT14I | CCWWGG | 1 cut(s) | 823 |
| EcoT38I | GRGCYC | 4 cut(s) | 299, 326, 444, 598 |
| ErhI | CCWWGG | 1 cut(s) | 823 |
| Esp3I | CGTCTC | 1 cut(s) | 91 |
| FaeI | CATG | 6 cut(s) | 448, 455, 775, 801, 827, 994 |
| FatI | CATG | 6 cut(s) | 444, 451, 771, 797, 823, 990 |
| FauNDI | CATATG | 2 cut(s) | 505, 1000 |
| FbaI | TGATCA | 1 cut(s) | 922 |
| FblI | GTMKAC | 2 cut(s) | 219, 652 |
| Fnu4HI | GCNGC | 6 cut(s) | 53, 132, 150, 498, 967, 982 |
| FokI | GGATG | 2 cut(s) | 732, 803 |
| FriOI | GRGCYC | 4 cut(s) | 299, 326, 444, 598 |
| Fsp4HI | GCNGC | 6 cut(s) | 53, 132, 150, 498, 967, 982 |
| FspBI | CTAG | 1 cut(s) | 170 |
| GlaI | GCGC | 1 cut(s) | 752 |
| GluI | GCNGC | 6 cut(s) | 53, 132, 150, 498, 967, 982 |
| GsaI | CCCAGC | 1 cut(s) | 389 |
| GsuI | CTGGAG | 1 cut(s) | 574 |
| HaeII | RGCGCY | 1 cut(s) | 754 |
| HaeIII | GGCC | 1 cut(s) | 297 |
| HapII | CCGG | 2 cut(s) | 393, 413 |
| HgaI | GACGC | 2 cut(s) | 225, 638 |
| HhaI | GCGC | 1 cut(s) | 753 |
| Hin1I | GRCGYC | 2 cut(s) | 236, 309 |
| Hin1II | CATG | 6 cut(s) | 448, 455, 775, 801, 827, 994 |
| Hin6I | GCGC | 1 cut(s) | 751 |
| HinP1I | GCGC | 1 cut(s) | 751 |
| HinfI | GANTC | 7 cut(s) | 70, 75, 212, 264, 396, 668, 794 |
| HpaII | CCGG | 2 cut(s) | 393, 413 |
| HphI | GGTGA | 1 cut(s) | 70 |
| Hpy166II | GTNNAC | 2 cut(s) | 220, 653 |
| Hpy188I | TCNGA | 5 cut(s) | 118, 263, 673, 715, 1020 |
| Hpy8I | GTNNAC | 2 cut(s) | 220, 653 |
| Hpy99I | CGWCG | 1 cut(s) | 241 |
| HpyAV | CCTTC | 2 cut(s) | 261, 542 |
| HpyCH4III | ACNGT | 3 cut(s) | 434, 602, 874 |
| HpyCH4IV | ACGT | 4 cut(s) | 222, 304, 309, 950 |
| HpyCH4V | TGCA | 2 cut(s) | 330, 735 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 233, 356, 748 |
| HpyF3I | CTNAG | 4 cut(s) | 269, 714, 743, 804 |
| HpySE526I | ACGT | 4 cut(s) | 222, 304, 309, 950 |
| Hsp92I | GRCGYC | 2 cut(s) | 236, 309 |
| Hsp92II | CATG | 6 cut(s) | 448, 455, 775, 801, 827, 994 |
| HspAI | GCGC | 1 cut(s) | 751 |
| Ksp22I | TGATCA | 1 cut(s) | 922 |
| Kzo9I | GATC | 1 cut(s) | 922 |
| LmnI | GCTCC | 2 cut(s) | 329, 415 |
| Lsp1109I | GCAGC | 5 cut(s) | 39, 161, 509, 978, 993 |
| LweI | GCATC | 3 cut(s) | 143, 346, 710 |
| MaeI | CTAG | 1 cut(s) | 170 |
| MaeII | ACGT | 4 cut(s) | 222, 304, 309, 950 |
| MaeIII | GTNAC | 2 cut(s) | 11, 305 |
| MalI | GATC | 1 cut(s) | 924 |
| MboI | GATC | 1 cut(s) | 922 |
| MfeI | CAATTG | 1 cut(s) | 883 |
| MhlI | GDGCHC | 4 cut(s) | 299, 326, 444, 598 |
| MluCI | AATT | 8 cut(s) | 125, 140, 553, 691, 760, 847, 883, 957 |
| MlyI | GAGTC | 3 cut(s) | 258, 662, 788 |
| MmeI | TCCRAC | 2 cut(s) | 141, 696 |
| MroXI | GAANNNNTTC | 1 cut(s) | 581 |
| MseI | TTAA | 4 cut(s) | 696, 813, 987, 1023 |
| MspA1I | CMGCKG | 2 cut(s) | 160, 969 |
| MspI | CCGG | 2 cut(s) | 393, 413 |
| MspR9I | CCNGG | 2 cut(s) | 420, 905 |
| MunI | CAATTG | 1 cut(s) | 883 |
| MvaI | CCWGG | 2 cut(s) | 420, 905 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 233, 356, 748 |
| NcoI | CCATGG | 1 cut(s) | 823 |
| NdeI | CATATG | 2 cut(s) | 505, 1000 |
| NdeII | GATC | 1 cut(s) | 922 |
| NlaIII | CATG | 6 cut(s) | 448, 455, 775, 801, 827, 994 |
| NlaIV | GGNNCC | 5 cut(s) | 296, 297, 325, 383, 417 |
| NmuCI | GTSAC | 2 cut(s) | 11, 305 |
| NspI | RCATGY | 1 cut(s) | 994 |
| NspV | TTCGAA | 1 cut(s) | 144 |
| PaeR7I | CTCGAG | 1 cut(s) | 281 |
| PagI | TCATGA | 1 cut(s) | 771 |
| PciI | ACATGT | 1 cut(s) | 990 |
| PdmI | GAANNNNTTC | 1 cut(s) | 581 |
| PfeI | GAWTC | 4 cut(s) | 70, 75, 212, 396 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 910 |
| PkrI | GCNGC | 6 cut(s) | 54, 133, 151, 499, 968, 983 |
| PleI | GAGTC | 3 cut(s) | 258, 662, 788 |
| PmaCI | CACGTG | 1 cut(s) | 305 |
| PmlI | CACGTG | 1 cut(s) | 305 |
| PpsI | GAGTC | 3 cut(s) | 258, 662, 788 |
| Ppu21I | YACGTR | 2 cut(s) | 305, 951 |
| PscI | ACATGT | 1 cut(s) | 990 |
| PshBI | ATTAAT | 1 cut(s) | 1023 |
| Psp124BI | GAGCTC | 2 cut(s) | 444, 598 |
| Psp6I | CCWGG | 2 cut(s) | 418, 903 |
| PspCI | CACGTG | 1 cut(s) | 305 |
| PspFI | CCCAGC | 1 cut(s) | 385 |
| PspGI | CCWGG | 2 cut(s) | 418, 903 |
| PspN4I | GGNNCC | 5 cut(s) | 296, 297, 325, 383, 417 |
| PspOMI | GGGCCC | 1 cut(s) | 295 |
| PspPI | GGNCC | 2 cut(s) | 295, 296 |
| PvuII | CAGCTG | 1 cut(s) | 969 |
| RsaI | GTAC | 2 cut(s) | 871, 953 |
| RsaNI | GTAC | 2 cut(s) | 870, 952 |
| SacI | GAGCTC | 2 cut(s) | 444, 598 |
| SaqAI | TTAA | 4 cut(s) | 696, 813, 987, 1023 |
| SatI | GCNGC | 6 cut(s) | 53, 132, 150, 498, 967, 982 |
| Sau3AI | GATC | 1 cut(s) | 922 |
| Sau96I | GGNCC | 2 cut(s) | 295, 296 |
| SchI | GAGTC | 3 cut(s) | 258, 662, 788 |
| ScrFI | CCNGG | 2 cut(s) | 420, 905 |
| SduI | GDGCHC | 4 cut(s) | 299, 326, 444, 598 |
| SfaNI | GCATC | 3 cut(s) | 143, 346, 710 |
| SfcI | CTRYAG | 2 cut(s) | 216, 484 |
| Sfr274I | CTCGAG | 1 cut(s) | 281 |
| SfuI | TTCGAA | 1 cut(s) | 144 |
| SlaI | CTCGAG | 1 cut(s) | 281 |
| SmlI | CTYRAG | 1 cut(s) | 281 |
| SmoI | CTYRAG | 1 cut(s) | 281 |
| Sse9I | AATT | 8 cut(s) | 125, 140, 553, 691, 760, 847, 883, 957 |
| SsiI | CCGC | 3 cut(s) | 5, 132, 160 |
| SspMI | CTAG | 1 cut(s) | 170 |
| SstI | GAGCTC | 2 cut(s) | 444, 598 |
| StyD4I | CCNGG | 2 cut(s) | 418, 903 |
| StyI | CCWWGG | 1 cut(s) | 823 |
| TaaI | ACNGT | 3 cut(s) | 434, 602, 874 |
| TaiI | ACGT | 4 cut(s) | 225, 307, 312, 953 |
| TaqI | TCGA | 5 cut(s) | 73, 89, 144, 210, 282 |
| TasI | AATT | 8 cut(s) | 125, 140, 553, 691, 760, 847, 883, 957 |
| TatI | WGTACW | 1 cut(s) | 869 |
| TauI | GCSGC | 1 cut(s) | 134 |
| TfiI | GAWTC | 4 cut(s) | 70, 75, 212, 396 |
| Tru1I | TTAA | 4 cut(s) | 696, 813, 987, 1023 |
| Tru9I | TTAA | 4 cut(s) | 696, 813, 987, 1023 |
| TseFI | GTSAC | 2 cut(s) | 11, 305 |
| TseI | GCWGC | 5 cut(s) | 52, 149, 497, 966, 981 |
| Tsp45I | GTSAC | 2 cut(s) | 11, 305 |
| TspDTI | ATGAA | 6 cut(s) | 94, 543, 549, 590, 693, 1017 |
| TspGWI | ACGGA | 2 cut(s) | 73, 858 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 910 |
| VspI | ATTAAT | 1 cut(s) | 1023 |
| XapI | RAATTY | 2 cut(s) | 691, 847 |
| XceI | RCATGY | 1 cut(s) | 994 |
| XhoI | CTCGAG | 1 cut(s) | 281 |
| XmiI | GTMKAC | 2 cut(s) | 219, 652 |
| XmnI | GAANNNNTTC | 1 cut(s) | 581 |
| XspI | CTAG | 1 cut(s) | 170 |
| ZraI | GACGTC | 1 cut(s) | 310 |