Peptidase, trypsin-like serine and cysteine proteases
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 423 |
| Acc36I | ACCTGC | 1 cut(s) | 423 |
| Acc65I | GGTACC | 1 cut(s) | 411 |
| AccB1I | GGYRCC | 1 cut(s) | 411 |
| AccBSI | CCGCTC | 1 cut(s) | 84 |
| AccII | CGCG | 2 cut(s) | 23, 447 |
| AciI | CCGC | 5 cut(s) | 84, 105, 129, 430, 445 |
| AcoI | YGGCCR | 2 cut(s) | 130, 421 |
| AcsI | RAATTY | 1 cut(s) | 453 |
| AcuI | CTGAAG | 2 cut(s) | 277, 278 |
| AcyI | GRCGYC | 1 cut(s) | 5 |
| AfaI | GTAC | 2 cut(s) | 161, 413 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 34, 156, 365 |
| AgeI | ACCGGT | 1 cut(s) | 365 |
| AjnI | CCWGG | 2 cut(s) | 98, 406 |
| AluBI | AGCT | 4 cut(s) | 19, 55, 155, 277 |
| AluI | AGCT | 4 cut(s) | 19, 55, 155, 277 |
| Alw26I | GTCTC | 1 cut(s) | 98 |
| AoxI | GGCC | 4 cut(s) | 102, 130, 421, 441 |
| ApeKI | GCWGC | 1 cut(s) | 306 |
| ApoI | RAATTY | 1 cut(s) | 453 |
| AsiGI | ACCGGT | 1 cut(s) | 365 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 491 |
| Asp718I | GGTACC | 1 cut(s) | 411 |
| AspLEI | GCGC | 1 cut(s) | 405 |
| AspS9I | GGNCC | 3 cut(s) | 46, 102, 442 |
| AsuHPI | GGTGA | 2 cut(s) | 88, 274 |
| AvaII | GGWCC | 1 cut(s) | 46 |
| BanI | GGYRCC | 1 cut(s) | 411 |
| BbvI | GCAGC | 1 cut(s) | 293 |
| BceAI | ACGGC | 1 cut(s) | 408 |
| BciT130I | CCWGG | 2 cut(s) | 100, 408 |
| BcoDI | GTCTC | 1 cut(s) | 98 |
| BfoI | RGCGCY | 1 cut(s) | 406 |
| BfuAI | ACCTGC | 1 cut(s) | 423 |
| BisI | GCNGC | 5 cut(s) | 85, 105, 130, 307, 430 |
| BlsI | GCNGC | 5 cut(s) | 86, 106, 131, 308, 431 |
| Bme1390I | CCNGG | 2 cut(s) | 100, 408 |
| Bme18I | GGWCC | 1 cut(s) | 46 |
| BmgT120I | GGNCC | 3 cut(s) | 46, 102, 442 |
| BmiI | GGNNCC | 2 cut(s) | 413, 494 |
| BmrFI | CCNGG | 2 cut(s) | 100, 408 |
| BmsI | GCATC | 1 cut(s) | 469 |
| BpuEI | CTTGAG | 1 cut(s) | 419 |
| Bsa29I | ATCGAT | 1 cut(s) | 336 |
| BsaHI | GRCGYC | 1 cut(s) | 5 |
| BsaJI | CCNNGG | 4 cut(s) | 99, 108, 406, 407 |
| BsaWI | WCCGGW | 1 cut(s) | 365 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 34, 156, 365 |
| Bse118I | RCCGGY | 1 cut(s) | 365 |
| Bse1I | ACTGG | 1 cut(s) | 307 |
| Bse3DI | GCAATG | 2 cut(s) | 240, 489 |
| BseBI | CCWGG | 2 cut(s) | 100, 408 |
| BseCI | ATCGAT | 1 cut(s) | 336 |
| BseDI | CCNNGG | 4 cut(s) | 99, 108, 406, 407 |
| BseGI | GGATG | 2 cut(s) | 481, 516 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 34, 156, 365 |
| BseMI | GCAATG | 2 cut(s) | 240, 489 |
| BseMII | CTCAG | 1 cut(s) | 139 |
| BseNI | ACTGG | 1 cut(s) | 307 |
| BseXI | GCAGC | 1 cut(s) | 293 |
| Bsh1236I | CGCG | 2 cut(s) | 23, 447 |
| Bsh1285I | CGRYCG | 1 cut(s) | 91 |
| BshFI | GGCC | 4 cut(s) | 104, 132, 423, 443 |
| BshNI | GGYRCC | 1 cut(s) | 411 |
| BshTI | ACCGGT | 1 cut(s) | 365 |
| BshVI | ATCGAT | 1 cut(s) | 336 |
| BsiEI | CGRYCG | 1 cut(s) | 91 |
| BsiSI | CCGG | 1 cut(s) | 366 |
| BslFI | GGGAC | 1 cut(s) | 514 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 34, 156, 365 |
| BsmAI | GTCTC | 1 cut(s) | 98 |
| BsmBI | CGTCTC | 1 cut(s) | 98 |
| BsmFI | GGGAC | 1 cut(s) | 514 |
| BsnI | GGCC | 4 cut(s) | 104, 132, 423, 443 |
| Bsp143I | GATC | 3 cut(s) | 88, 198, 337 |
| Bsp68I | TCGCGA | 1 cut(s) | 23 |
| BspACI | CCGC | 5 cut(s) | 84, 105, 129, 430, 445 |
| BspANI | GGCC | 4 cut(s) | 104, 132, 423, 443 |
| BspCNI | CTCAG | 1 cut(s) | 138 |
| BspDI | ATCGAT | 1 cut(s) | 336 |
| BspFNI | CGCG | 2 cut(s) | 23, 447 |
| BspLI | GGNNCC | 2 cut(s) | 413, 494 |
| BspMI | ACCTGC | 1 cut(s) | 423 |
| BspT107I | GGYRCC | 1 cut(s) | 411 |
| BsrBI | CCGCTC | 1 cut(s) | 84 |
| BsrDI | GCAATG | 2 cut(s) | 240, 489 |
| BsrFI | RCCGGY | 1 cut(s) | 365 |
| BsrI | ACTGG | 1 cut(s) | 307 |
| BssAI | RCCGGY | 1 cut(s) | 365 |
| BssECI | CCNNGG | 4 cut(s) | 99, 108, 406, 407 |
| BssMI | GATC | 3 cut(s) | 88, 198, 337 |
| BssNI | GRCGYC | 1 cut(s) | 5 |
| BssT1I | CCWWGG | 1 cut(s) | 108 |
| Bst2UI | CCWGG | 2 cut(s) | 100, 408 |
| Bst4CI | ACNGT | 5 cut(s) | 166, 217, 243, 377, 416 |
| BstACI | GRCGYC | 1 cut(s) | 5 |
| BstC8I | GCNNGC | 3 cut(s) | 21, 205, 445 |
| BstDEI | CTNAG | 1 cut(s) | 125 |
| BstF5I | GGATG | 2 cut(s) | 481, 516 |
| BstFNI | CGCG | 2 cut(s) | 23, 447 |
| BstH2I | RGCGCY | 1 cut(s) | 406 |
| BstHHI | GCGC | 1 cut(s) | 405 |
| BstKTI | GATC | 3 cut(s) | 91, 201, 340 |
| BstMAI | GTCTC | 1 cut(s) | 98 |
| BstMBI | GATC | 3 cut(s) | 88, 198, 337 |
| BstMCI | CGRYCG | 1 cut(s) | 91 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 84, 429, 435, 444 |
| BstNI | CCWGG | 2 cut(s) | 100, 408 |
| BstSCI | CCNGG | 2 cut(s) | 98, 406 |
| BstUI | CGCG | 2 cut(s) | 23, 447 |
| BstV1I | GCAGC | 1 cut(s) | 293 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 115 |
| Bsu15I | ATCGAT | 1 cut(s) | 336 |
| BsuRI | GGCC | 4 cut(s) | 104, 132, 423, 443 |
| BsuTUI | ATCGAT | 1 cut(s) | 336 |
| BtsCI | GGATG | 2 cut(s) | 481, 516 |
| BtsIMutI | CAGTG | 3 cut(s) | 162, 239, 301 |
| BtuMI | TCGCGA | 1 cut(s) | 23 |
| BveI | ACCTGC | 1 cut(s) | 423 |
| Cac8I | GCNNGC | 3 cut(s) | 21, 205, 445 |
| CfoI | GCGC | 1 cut(s) | 405 |
| Cfr10I | RCCGGY | 1 cut(s) | 365 |
| Cfr13I | GGNCC | 3 cut(s) | 46, 102, 442 |
| ClaI | ATCGAT | 1 cut(s) | 336 |
| Csp6I | GTAC | 2 cut(s) | 160, 412 |
| CspAI | ACCGGT | 1 cut(s) | 365 |
| CviAII | CATG | 1 cut(s) | 31 |
| CviQI | GTAC | 2 cut(s) | 160, 412 |
| DdeI | CTNAG | 1 cut(s) | 125 |
| DpnI | GATC | 3 cut(s) | 90, 200, 339 |
| DpnII | GATC | 3 cut(s) | 88, 198, 337 |
| EaeI | YGGCCR | 2 cut(s) | 130, 421 |
| Eco130I | CCWWGG | 1 cut(s) | 108 |
| Eco47I | GGWCC | 1 cut(s) | 46 |
| Eco57I | CTGAAG | 2 cut(s) | 277, 278 |
| EcoRI | GAATTC | 1 cut(s) | 453 |
| EcoRII | CCWGG | 2 cut(s) | 98, 406 |
| EcoT14I | CCWWGG | 1 cut(s) | 108 |
| ErhI | CCWWGG | 1 cut(s) | 108 |
| Esp3I | CGTCTC | 1 cut(s) | 98 |
| FaeI | CATG | 1 cut(s) | 34 |
| FaiI | YATR | 7 cut(s) | 32, 210, 273, 333, 354, 380, 390 |
| FaqI | GGGAC | 1 cut(s) | 514 |
| FatI | CATG | 1 cut(s) | 30 |
| FauI | CCCGC | 1 cut(s) | 452 |
| Fnu4HI | GCNGC | 5 cut(s) | 85, 105, 130, 307, 430 |
| FokI | GGATG | 2 cut(s) | 488, 503 |
| Fsp4HI | GCNGC | 5 cut(s) | 85, 105, 130, 307, 430 |
| GlaI | GCGC | 1 cut(s) | 404 |
| GluI | GCNGC | 5 cut(s) | 85, 105, 130, 307, 430 |
| HaeII | RGCGCY | 1 cut(s) | 406 |
| HaeIII | GGCC | 4 cut(s) | 104, 132, 423, 443 |
| HapII | CCGG | 1 cut(s) | 366 |
| HhaI | GCGC | 1 cut(s) | 405 |
| Hin1I | GRCGYC | 1 cut(s) | 5 |
| Hin1II | CATG | 1 cut(s) | 34 |
| Hin6I | GCGC | 1 cut(s) | 403 |
| HinP1I | GCGC | 1 cut(s) | 403 |
| HinfI | GANTC | 3 cut(s) | 180, 315, 513 |
| HpaII | CCGG | 1 cut(s) | 366 |
| HphI | GGTGA | 2 cut(s) | 88, 274 |
| Hpy166II | GTNNAC | 1 cut(s) | 162 |
| Hpy188I | TCNGA | 2 cut(s) | 10, 179 |
| Hpy188III | TCNNGA | 4 cut(s) | 22, 49, 326, 398 |
| Hpy8I | GTNNAC | 1 cut(s) | 162 |
| Hpy99I | CGWCG | 3 cut(s) | 10, 77, 429 |
| HpyAV | CCTTC | 3 cut(s) | 253, 259, 369 |
| HpyCH4III | ACNGT | 5 cut(s) | 166, 217, 243, 377, 416 |
| HpyCH4V | TGCA | 2 cut(s) | 438, 482 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 84, 429, 435, 444 |
| HpyF3I | CTNAG | 1 cut(s) | 125 |
| Hsp92I | GRCGYC | 1 cut(s) | 5 |
| Hsp92II | CATG | 1 cut(s) | 34 |
| HspAI | GCGC | 1 cut(s) | 403 |
| KpnI | GGTACC | 1 cut(s) | 415 |
| Kzo9I | GATC | 3 cut(s) | 88, 198, 337 |
| LmnI | GCTCC | 2 cut(s) | 118, 328 |
| Lsp1109I | GCAGC | 1 cut(s) | 293 |
| LweI | GCATC | 1 cut(s) | 469 |
| MaeIII | GTNAC | 3 cut(s) | 211, 237, 466 |
| MalI | GATC | 3 cut(s) | 90, 200, 339 |
| MbiI | CCGCTC | 1 cut(s) | 84 |
| MboI | GATC | 3 cut(s) | 88, 198, 337 |
| MboII | GAAGA | 2 cut(s) | 25, 208 |
| MfeI | CAATTG | 1 cut(s) | 537 |
| MluCI | AATT | 3 cut(s) | 345, 453, 537 |
| MlyI | GAGTC | 1 cut(s) | 522 |
| MmeI | TCCRAC | 2 cut(s) | 225, 430 |
| MnlI | CCTC | 4 cut(s) | 27, 71, 162, 502 |
| MroXI | GAANNNNTTC | 1 cut(s) | 491 |
| MseI | TTAA | 2 cut(s) | 348, 522 |
| MspA1I | CMGCKG | 1 cut(s) | 129 |
| MspI | CCGG | 1 cut(s) | 366 |
| MspR9I | CCNGG | 2 cut(s) | 100, 408 |
| MunI | CAATTG | 1 cut(s) | 537 |
| MvaI | CCWGG | 2 cut(s) | 100, 408 |
| MvnI | CGCG | 2 cut(s) | 23, 447 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 84, 429, 435, 444 |
| NdeII | GATC | 3 cut(s) | 88, 198, 337 |
| NlaIII | CATG | 1 cut(s) | 34 |
| NlaIV | GGNNCC | 2 cut(s) | 413, 494 |
| NmuCI | GTSAC | 1 cut(s) | 237 |
| NruI | TCGCGA | 1 cut(s) | 23 |
| PaqCI | CACCTGC | 1 cut(s) | 423 |
| PasI | CCCWGGG | 1 cut(s) | 407 |
| PdmI | GAANNNNTTC | 1 cut(s) | 491 |
| PfeI | GAWTC | 2 cut(s) | 180, 315 |
| PinAI | ACCGGT | 1 cut(s) | 365 |
| PkrI | GCNGC | 5 cut(s) | 86, 106, 131, 308, 431 |
| Ple19I | CGATCG | 1 cut(s) | 91 |
| PleI | GAGTC | 1 cut(s) | 521 |
| PpsI | GAGTC | 1 cut(s) | 521 |
| Psp6I | CCWGG | 2 cut(s) | 98, 406 |
| PspGI | CCWGG | 2 cut(s) | 98, 406 |
| PspN4I | GGNNCC | 2 cut(s) | 413, 494 |
| PspPI | GGNCC | 3 cut(s) | 46, 102, 442 |
| PvuI | CGATCG | 1 cut(s) | 91 |
| RruI | TCGCGA | 1 cut(s) | 23 |
| RsaI | GTAC | 2 cut(s) | 161, 413 |
| RsaNI | GTAC | 2 cut(s) | 160, 412 |
| SaqAI | TTAA | 2 cut(s) | 348, 522 |
| SatI | GCNGC | 5 cut(s) | 85, 105, 130, 307, 430 |
| Sau3AI | GATC | 3 cut(s) | 88, 198, 337 |
| Sau96I | GGNCC | 3 cut(s) | 46, 102, 442 |
| SchI | GAGTC | 1 cut(s) | 522 |
| ScrFI | CCNGG | 2 cut(s) | 100, 408 |
| SetI | ASST | 9 cut(s) | 21, 48, 57, 114, 157, 173, 264, 279, 437 |
| SfaNI | GCATC | 1 cut(s) | 469 |
| SinI | GGWCC | 1 cut(s) | 46 |
| SmlI | CTYRAG | 1 cut(s) | 398 |
| SmoI | CTYRAG | 1 cut(s) | 398 |
| Sse9I | AATT | 3 cut(s) | 345, 453, 537 |
| SsiI | CCGC | 5 cut(s) | 84, 105, 129, 430, 445 |
| StyD4I | CCNGG | 2 cut(s) | 98, 406 |
| StyI | CCWWGG | 1 cut(s) | 108 |
| TaaI | ACNGT | 5 cut(s) | 166, 217, 243, 377, 416 |
| TaqI | TCGA | 1 cut(s) | 336 |
| TasI | AATT | 3 cut(s) | 345, 453, 537 |
| TauI | GCSGC | 4 cut(s) | 87, 107, 132, 432 |
| TfiI | GAWTC | 2 cut(s) | 180, 315 |
| Tru1I | TTAA | 2 cut(s) | 348, 522 |
| Tru9I | TTAA | 2 cut(s) | 348, 522 |
| TscAI | CASTG | 3 cut(s) | 169, 246, 301 |
| TseFI | GTSAC | 1 cut(s) | 237 |
| TseI | GCWGC | 1 cut(s) | 306 |
| Tsp45I | GTSAC | 1 cut(s) | 237 |
| TspDTI | ATGAA | 1 cut(s) | 500 |
| TspGWI | ACGGA | 1 cut(s) | 61 |
| TspRI | CASTG | 3 cut(s) | 169, 246, 301 |
| VpaK11BI | GGWCC | 1 cut(s) | 46 |
| XapI | RAATTY | 1 cut(s) | 453 |
| XmnI | GAANNNNTTC | 1 cut(s) | 491 |