Peptidase, trypsin-like serine and cysteine proteases
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 3 cut(s) | 351, 968, 1027 |
| AciI | CCGC | 4 cut(s) | 160, 738, 748, 994 |
| AclI | AACGTT | 1 cut(s) | 94 |
| AclWI | GGATC | 2 cut(s) | 64, 96 |
| AcoI | YGGCCR | 2 cut(s) | 3, 547 |
| AcsI | RAATTY | 2 cut(s) | 125, 621 |
| AcuI | CTGAAG | 2 cut(s) | 42, 435 |
| AfaI | GTAC | 1 cut(s) | 116 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 223, 239, 709, 903 |
| AflIII | ACRYGT | 1 cut(s) | 804 |
| AgsI | TTSAA | 2 cut(s) | 146, 583 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 663 |
| AjiI | CACGTC | 1 cut(s) | 805 |
| AjnI | CCWGG | 1 cut(s) | 544 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 908, 940 |
| AluBI | AGCT | 4 cut(s) | 152, 265, 518, 826 |
| AluI | AGCT | 4 cut(s) | 152, 265, 518, 826 |
| Alw21I | GWGCWC | 1 cut(s) | 520 |
| Alw26I | GTCTC | 1 cut(s) | 237 |
| AlwI | GGATC | 2 cut(s) | 64, 96 |
| AoxI | GGCC | 2 cut(s) | 3, 547 |
| ApeKI | GCWGC | 4 cut(s) | 52, 149, 482, 981 |
| ApoI | RAATTY | 2 cut(s) | 125, 621 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 110, 142 |
| AseI | ATTAAT | 1 cut(s) | 1038 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 503 |
| AspS9I | GGNCC | 1 cut(s) | 700 |
| AsuC2I | CCSGG | 1 cut(s) | 288 |
| AsuHPI | GGTGA | 2 cut(s) | 70, 863 |
| AvaII | GGWCC | 1 cut(s) | 700 |
| BalI | TGGCCA | 1 cut(s) | 549 |
| BanII | GRGCYC | 2 cut(s) | 293, 520 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 15, 47, 1011, 1043 |
| BauI | CACGAG | 1 cut(s) | 814 |
| Bbv12I | GWGCWC | 1 cut(s) | 520 |
| BbvI | GCAGC | 4 cut(s) | 39, 161, 494, 993 |
| BccI | CCATC | 1 cut(s) | 113 |
| BceAI | ACGGC | 3 cut(s) | 217, 440, 881 |
| BciT130I | CCWGG | 1 cut(s) | 546 |
| BciVI | GTATCC | 1 cut(s) | 220 |
| BclI | TGATCA | 2 cut(s) | 673, 937 |
| BcnI | CCSGG | 1 cut(s) | 288 |
| BcoDI | GTCTC | 1 cut(s) | 237 |
| BfaI | CTAG | 2 cut(s) | 218, 308 |
| BfuI | GTATCC | 1 cut(s) | 220 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 309 |
| BisI | GCNGC | 4 cut(s) | 53, 150, 483, 982 |
| BlsI | GCNGC | 4 cut(s) | 54, 151, 484, 983 |
| Bme1390I | CCNGG | 2 cut(s) | 288, 546 |
| Bme18I | GGWCC | 1 cut(s) | 700 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 663 |
| BmgBI | CACGTC | 1 cut(s) | 805 |
| BmgT120I | GGNCC | 1 cut(s) | 700 |
| BmiI | GGNNCC | 1 cut(s) | 368 |
| BmrFI | CCNGG | 2 cut(s) | 288, 546 |
| Bpu10I | CCTNAGC | 2 cut(s) | 399, 428 |
| BpuEI | CTTGAG | 1 cut(s) | 532 |
| BpuMI | CCSGG | 1 cut(s) | 288 |
| BsaBI | GATNNNNATC | 1 cut(s) | 612 |
| BsaJI | CCNNGG | 2 cut(s) | 462, 544 |
| BsaWI | WCCGGW | 2 cut(s) | 848, 878 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 236, 266, 908, 938 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 223, 239, 709, 903 |
| Bse118I | RCCGGY | 1 cut(s) | 984 |
| Bse3DI | GCAATG | 1 cut(s) | 325 |
| Bse8I | GATNNNNATC | 1 cut(s) | 612 |
| BseBI | CCWGG | 1 cut(s) | 546 |
| BseDI | CCNNGG | 2 cut(s) | 462, 544 |
| BseGI | GGATG | 1 cut(s) | 728 |
| BseJI | GATNNNNATC | 1 cut(s) | 612 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 223, 239, 709, 903 |
| BseMI | GCAATG | 1 cut(s) | 325 |
| BseMII | CTCAG | 3 cut(s) | 257, 442, 501 |
| BseRI | GAGGAG | 1 cut(s) | 662 |
| BseXI | GCAGC | 4 cut(s) | 39, 161, 494, 993 |
| BseYI | CCCAGC | 1 cut(s) | 370 |
| BshFI | GGCC | 2 cut(s) | 5, 549 |
| BsiHKAI | GWGCWC | 1 cut(s) | 520 |
| BsiSI | CCGG | 5 cut(s) | 255, 287, 849, 879, 985 |
| BslFI | GGGAC | 1 cut(s) | 586 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 223, 239, 709, 903 |
| BsmAI | GTCTC | 1 cut(s) | 237 |
| BsmFI | GGGAC | 1 cut(s) | 586 |
| BsnI | GGCC | 2 cut(s) | 5, 549 |
| Bsp1286I | GDGCHC | 2 cut(s) | 293, 520 |
| Bsp143I | GATC | 5 cut(s) | 69, 101, 673, 887, 937 |
| BspACI | CCGC | 4 cut(s) | 160, 738, 748, 994 |
| BspANI | GGCC | 2 cut(s) | 5, 549 |
| BspCNI | CTCAG | 3 cut(s) | 258, 441, 500 |
| BspHI | TCATGA | 2 cut(s) | 676, 789 |
| BspLI | GGNNCC | 1 cut(s) | 368 |
| BspPI | GGATC | 2 cut(s) | 64, 96 |
| BsrDI | GCAATG | 1 cut(s) | 325 |
| BsrFI | RCCGGY | 1 cut(s) | 984 |
| BssAI | RCCGGY | 1 cut(s) | 984 |
| BssECI | CCNNGG | 2 cut(s) | 462, 544 |
| BssMI | GATC | 5 cut(s) | 69, 101, 673, 887, 937 |
| BssNAI | GTATAC | 1 cut(s) | 969 |
| BssSI | CACGAG | 1 cut(s) | 814 |
| Bst1107I | GTATAC | 1 cut(s) | 969 |
| Bst2BI | CACGAG | 1 cut(s) | 814 |
| Bst2UI | CCWGG | 1 cut(s) | 546 |
| Bst6I | CTCTTC | 3 cut(s) | 486, 942, 1014 |
| BstC8I | GCNNGC | 4 cut(s) | 445, 986, 990, 994 |
| BstDEI | CTNAG | 6 cut(s) | 170, 266, 399, 428, 487, 822 |
| BstEII | GGTNACC | 2 cut(s) | 851, 874 |
| BstF5I | GGATG | 1 cut(s) | 728 |
| BstKTI | GATC | 5 cut(s) | 72, 104, 676, 890, 940 |
| BstMAI | GTCTC | 1 cut(s) | 237 |
| BstMBI | GATC | 5 cut(s) | 69, 101, 673, 887, 937 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 309 |
| BstNI | CCWGG | 1 cut(s) | 546 |
| BstPI | GGTNACC | 2 cut(s) | 851, 874 |
| BstSCI | CCNGG | 2 cut(s) | 286, 544 |
| BstV1I | GCAGC | 4 cut(s) | 39, 161, 494, 993 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 545 |
| BstZ17I | GTATAC | 1 cut(s) | 969 |
| BsuI | GTATCC | 1 cut(s) | 220 |
| BsuRI | GGCC | 2 cut(s) | 5, 549 |
| BtrI | CACGTC | 1 cut(s) | 805 |
| BtsCI | GGATG | 1 cut(s) | 728 |
| Cac8I | GCNNGC | 4 cut(s) | 445, 986, 990, 994 |
| CciI | TCATGA | 2 cut(s) | 676, 789 |
| Cfr10I | RCCGGY | 1 cut(s) | 984 |
| Cfr13I | GGNCC | 1 cut(s) | 700 |
| CseI | GACGC | 2 cut(s) | 653, 987 |
| Csp6I | GTAC | 1 cut(s) | 115 |
| CviAII | CATG | 8 cut(s) | 315, 677, 684, 704, 790, 964, 1006, 1014 |
| CviQI | GTAC | 1 cut(s) | 115 |
| DdeI | CTNAG | 6 cut(s) | 170, 266, 399, 428, 487, 822 |
| DpnI | GATC | 5 cut(s) | 71, 103, 675, 889, 939 |
| DpnII | GATC | 5 cut(s) | 69, 101, 673, 887, 937 |
| DriI | GACNNNNNGTC | 1 cut(s) | 663 |
| EaeI | YGGCCR | 2 cut(s) | 3, 547 |
| Eam1104I | CTCTTC | 3 cut(s) | 486, 942, 1014 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 663 |
| EarI | CTCTTC | 3 cut(s) | 486, 942, 1014 |
| Ecl136II | GAGCTC | 1 cut(s) | 518 |
| Eco24I | GRGCYC | 2 cut(s) | 293, 520 |
| Eco47I | GGWCC | 1 cut(s) | 700 |
| Eco53kI | GAGCTC | 1 cut(s) | 518 |
| Eco57I | CTGAAG | 2 cut(s) | 42, 435 |
| Eco91I | GGTNACC | 2 cut(s) | 851, 874 |
| EcoICRI | GAGCTC | 1 cut(s) | 518 |
| EcoO65I | GGTNACC | 2 cut(s) | 851, 874 |
| EcoRII | CCWGG | 1 cut(s) | 544 |
| EcoT38I | GRGCYC | 2 cut(s) | 293, 520 |
| FaeI | CATG | 8 cut(s) | 318, 680, 687, 707, 793, 967, 1009, 1017 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 609, 641 |
| FaqI | GGGAC | 1 cut(s) | 586 |
| FatI | CATG | 8 cut(s) | 314, 676, 683, 703, 789, 963, 1005, 1013 |
| FauI | CCCGC | 1 cut(s) | 1001 |
| FbaI | TGATCA | 2 cut(s) | 673, 937 |
| FblI | GTMKAC | 3 cut(s) | 351, 968, 1027 |
| Fnu4HI | GCNGC | 4 cut(s) | 53, 150, 483, 982 |
| FokI | GGATG | 1 cut(s) | 735 |
| FriOI | GRGCYC | 2 cut(s) | 293, 520 |
| Fsp4HI | GCNGC | 4 cut(s) | 53, 150, 483, 982 |
| FspBI | CTAG | 2 cut(s) | 218, 308 |
| GluI | GCNGC | 4 cut(s) | 53, 150, 483, 982 |
| GsaI | CCCAGC | 1 cut(s) | 374 |
| HaeIII | GGCC | 2 cut(s) | 5, 549 |
| HapII | CCGG | 5 cut(s) | 255, 287, 849, 879, 985 |
| HgaI | GACGC | 2 cut(s) | 653, 987 |
| Hin1II | CATG | 8 cut(s) | 318, 680, 687, 707, 793, 967, 1009, 1017 |
| HincII | GTYRAC | 2 cut(s) | 352, 422 |
| HindII | GTYRAC | 2 cut(s) | 352, 422 |
| HinfI | GANTC | 8 cut(s) | 75, 212, 381, 574, 812, 973, 1009, 1024 |
| HpaII | CCGG | 5 cut(s) | 255, 287, 849, 879, 985 |
| HphI | GGTGA | 2 cut(s) | 70, 863 |
| Hpy166II | GTNNAC | 6 cut(s) | 352, 422, 694, 819, 969, 1028 |
| Hpy188I | TCNGA | 5 cut(s) | 74, 101, 415, 490, 1035 |
| Hpy188III | TCNNGA | 5 cut(s) | 364, 617, 655, 677, 790 |
| Hpy8I | GTNNAC | 6 cut(s) | 352, 422, 694, 819, 969, 1028 |
| Hpy99I | CGWCG | 1 cut(s) | 981 |
| HpyAV | CCTTC | 2 cut(s) | 410, 946 |
| HpyCH4IV | ACGT | 3 cut(s) | 94, 522, 804 |
| HpyCH4V | TGCA | 1 cut(s) | 318 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 309 |
| HpyF3I | CTNAG | 6 cut(s) | 170, 266, 399, 428, 487, 822 |
| HpySE526I | ACGT | 3 cut(s) | 94, 522, 804 |
| Hsp92II | CATG | 8 cut(s) | 318, 680, 687, 707, 793, 967, 1009, 1017 |
| KroI | GCCGGC | 1 cut(s) | 984 |
| KroNI | GCCGGC | 1 cut(s) | 986 |
| Ksp22I | TGATCA | 2 cut(s) | 673, 937 |
| Kzo9I | GATC | 5 cut(s) | 69, 101, 673, 887, 937 |
| Lsp1109I | GCAGC | 4 cut(s) | 39, 161, 494, 993 |
| MaeI | CTAG | 2 cut(s) | 218, 308 |
| MaeII | ACGT | 3 cut(s) | 94, 522, 804 |
| MaeIII | GTNAC | 2 cut(s) | 851, 874 |
| MalI | GATC | 5 cut(s) | 71, 103, 675, 889, 939 |
| MboI | GATC | 5 cut(s) | 69, 101, 673, 887, 937 |
| MboII | GAAGA | 8 cut(s) | 35, 178, 376, 503, 511, 837, 959, 1031 |
| MhlI | GDGCHC | 2 cut(s) | 293, 520 |
| MlsI | TGGCCA | 1 cut(s) | 549 |
| MluCI | AATT | 5 cut(s) | 125, 140, 591, 621, 1039 |
| MluNI | TGGCCA | 1 cut(s) | 549 |
| MlyI | GAGTC | 4 cut(s) | 568, 806, 967, 1033 |
| MmeI | TCCRAC | 1 cut(s) | 562 |
| Mox20I | TGGCCA | 1 cut(s) | 549 |
| MroNI | GCCGGC | 1 cut(s) | 984 |
| MroXI | GAANNNNTTC | 1 cut(s) | 503 |
| MscI | TGGCCA | 1 cut(s) | 549 |
| MseI | TTAA | 3 cut(s) | 831, 930, 1038 |
| Msp20I | TGGCCA | 1 cut(s) | 549 |
| MspA1I | CMGCKG | 2 cut(s) | 160, 748 |
| MspI | CCGG | 5 cut(s) | 255, 287, 849, 879, 985 |
| MspR9I | CCNGG | 2 cut(s) | 288, 546 |
| MvaI | CCWGG | 1 cut(s) | 546 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 309 |
| NaeI | GCCGGC | 1 cut(s) | 986 |
| NciI | CCSGG | 1 cut(s) | 288 |
| NdeII | GATC | 5 cut(s) | 69, 101, 673, 887, 937 |
| NgoMIV | GCCGGC | 1 cut(s) | 984 |
| NlaIII | CATG | 8 cut(s) | 318, 680, 687, 707, 793, 967, 1009, 1017 |
| NlaIV | GGNNCC | 1 cut(s) | 368 |
| NmeAIII | GCCGAG | 1 cut(s) | 272 |
| NmuCI | GTSAC | 1 cut(s) | 851 |
| PagI | TCATGA | 2 cut(s) | 676, 789 |
| PdiI | GCCGGC | 1 cut(s) | 986 |
| PdmI | GAANNNNTTC | 1 cut(s) | 503 |
| PfeI | GAWTC | 4 cut(s) | 75, 212, 381, 1009 |
| PkrI | GCNGC | 4 cut(s) | 54, 151, 484, 983 |
| PleI | GAGTC | 4 cut(s) | 568, 806, 967, 1032 |
| PpsI | GAGTC | 4 cut(s) | 568, 806, 967, 1032 |
| PshBI | ATTAAT | 1 cut(s) | 1038 |
| Psp124BI | GAGCTC | 1 cut(s) | 520 |
| Psp1406I | AACGTT | 1 cut(s) | 94 |
| Psp6I | CCWGG | 1 cut(s) | 544 |
| PspEI | GGTNACC | 2 cut(s) | 851, 874 |
| PspFI | CCCAGC | 1 cut(s) | 370 |
| PspGI | CCWGG | 1 cut(s) | 544 |
| PspN4I | GGNNCC | 1 cut(s) | 368 |
| PspPI | GGNCC | 1 cut(s) | 700 |
| RsaI | GTAC | 1 cut(s) | 116 |
| RsaNI | GTAC | 1 cut(s) | 115 |
| SacI | GAGCTC | 1 cut(s) | 520 |
| SalI | GTCGAC | 1 cut(s) | 350 |
| SaqAI | TTAA | 3 cut(s) | 831, 930, 1038 |
| SatI | GCNGC | 4 cut(s) | 53, 150, 483, 982 |
| Sau3AI | GATC | 5 cut(s) | 69, 101, 673, 887, 937 |
| Sau96I | GGNCC | 1 cut(s) | 700 |
| SchI | GAGTC | 4 cut(s) | 568, 806, 967, 1033 |
| ScrFI | CCNGG | 2 cut(s) | 288, 546 |
| SduI | GDGCHC | 2 cut(s) | 293, 520 |
| SinI | GGWCC | 1 cut(s) | 700 |
| SmlI | CTYRAG | 1 cut(s) | 511 |
| SmoI | CTYRAG | 1 cut(s) | 511 |
| Sse9I | AATT | 5 cut(s) | 125, 140, 591, 621, 1039 |
| SsiI | CCGC | 4 cut(s) | 160, 738, 748, 994 |
| SspMI | CTAG | 2 cut(s) | 218, 308 |
| SstI | GAGCTC | 1 cut(s) | 520 |
| StyD4I | CCNGG | 2 cut(s) | 286, 544 |
| TaiI | ACGT | 3 cut(s) | 97, 525, 807 |
| TaqI | TCGA | 5 cut(s) | 210, 279, 351, 777, 976 |
| TasI | AATT | 5 cut(s) | 125, 140, 591, 621, 1039 |
| TfiI | GAWTC | 4 cut(s) | 75, 212, 381, 1009 |
| Tru1I | TTAA | 3 cut(s) | 831, 930, 1038 |
| Tru9I | TTAA | 3 cut(s) | 831, 930, 1038 |
| TseFI | GTSAC | 1 cut(s) | 851 |
| TseI | GCWGC | 4 cut(s) | 52, 149, 482, 981 |
| Tsp45I | GTSAC | 1 cut(s) | 851 |
| TspDTI | ATGAA | 2 cut(s) | 512, 584 |
| TspGWI | ACGGA | 1 cut(s) | 73 |
| VpaK11BI | GGWCC | 1 cut(s) | 700 |
| VspI | ATTAAT | 1 cut(s) | 1038 |
| XapI | RAATTY | 2 cut(s) | 125, 621 |
| XmiI | GTMKAC | 3 cut(s) | 351, 968, 1027 |
| XmnI | GAANNNNTTC | 1 cut(s) | 503 |
| XspI | CTAG | 2 cut(s) | 218, 308 |