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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 163 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 362 |
| AfeI | AGCGCT | 1 cut(s) | 213 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 362, 387 |
| AgsI | TTSAA | 2 cut(s) | 110, 248 |
| AjnI | CCWGG | 2 cut(s) | 78, 114 |
| AluBI | AGCT | 2 cut(s) | 126, 227 |
| AluI | AGCT | 2 cut(s) | 126, 227 |
| Alw21I | GWGCWC | 1 cut(s) | 187 |
| Alw26I | GTCTC | 2 cut(s) | 452, 483 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 362 |
| Aor51HI | AGCGCT | 1 cut(s) | 213 |
| AoxI | GGCC | 2 cut(s) | 83, 446 |
| ApeKI | GCWGC | 4 cut(s) | 120, 123, 475, 478 |
| AspLEI | GCGC | 1 cut(s) | 214 |
| AspS9I | GGNCC | 3 cut(s) | 83, 378, 428 |
| AsuC2I | CCSGG | 1 cut(s) | 29 |
| AvaII | GGWCC | 2 cut(s) | 378, 428 |
| BauI | CACGAG | 1 cut(s) | 345 |
| Bbv12I | GWGCWC | 1 cut(s) | 187 |
| BbvI | GCAGC | 4 cut(s) | 132, 135, 487, 490 |
| BciT130I | CCWGG | 2 cut(s) | 80, 116 |
| BciVI | GTATCC | 1 cut(s) | 345 |
| BcnI | CCSGG | 1 cut(s) | 29 |
| BcoDI | GTCTC | 2 cut(s) | 452, 483 |
| BfaI | CTAG | 1 cut(s) | 159 |
| BfmI | CTRYAG | 1 cut(s) | 222 |
| BfoI | RGCGCY | 1 cut(s) | 215 |
| BfuAI | ACCTGC | 1 cut(s) | 163 |
| BfuI | GTATCC | 1 cut(s) | 345 |
| BisI | GCNGC | 4 cut(s) | 121, 124, 476, 479 |
| BlsI | GCNGC | 4 cut(s) | 122, 125, 477, 480 |
| Bme1390I | CCNGG | 3 cut(s) | 29, 80, 116 |
| Bme18I | GGWCC | 2 cut(s) | 378, 428 |
| BmgT120I | GGNCC | 3 cut(s) | 83, 378, 428 |
| BmiI | GGNNCC | 1 cut(s) | 84 |
| BmrFI | CCNGG | 3 cut(s) | 29, 80, 116 |
| BmrI | ACTGGG | 2 cut(s) | 350, 396 |
| BmuI | ACTGGG | 2 cut(s) | 350, 396 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 48, 80 |
| BpuEI | CTTGAG | 1 cut(s) | 455 |
| BpuMI | CCSGG | 1 cut(s) | 29 |
| BsaJI | CCNNGG | 3 cut(s) | 28, 79, 115 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 362, 387 |
| Bse1I | ACTGG | 3 cut(s) | 356, 367, 391 |
| BseBI | CCWGG | 2 cut(s) | 80, 116 |
| BseDI | CCNNGG | 3 cut(s) | 28, 79, 115 |
| BseGI | GGATG | 2 cut(s) | 275, 284 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 362, 387 |
| BseNI | ACTGG | 3 cut(s) | 356, 367, 391 |
| BseXI | GCAGC | 4 cut(s) | 132, 135, 487, 490 |
| BshFI | GGCC | 2 cut(s) | 85, 448 |
| BsiHKAI | GWGCWC | 1 cut(s) | 187 |
| BsiSI | CCGG | 1 cut(s) | 28 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 362, 387 |
| BsmAI | GTCTC | 2 cut(s) | 452, 483 |
| BsnI | GGCC | 2 cut(s) | 85, 448 |
| Bsp1286I | GDGCHC | 1 cut(s) | 187 |
| Bsp143I | GATC | 2 cut(s) | 194, 466 |
| BspANI | GGCC | 2 cut(s) | 85, 448 |
| BspLI | GGNNCC | 1 cut(s) | 84 |
| BspMI | ACCTGC | 1 cut(s) | 163 |
| BsrI | ACTGG | 3 cut(s) | 356, 367, 391 |
| BssECI | CCNNGG | 3 cut(s) | 28, 79, 115 |
| BssMI | GATC | 2 cut(s) | 194, 466 |
| BssSI | CACGAG | 1 cut(s) | 345 |
| Bst2BI | CACGAG | 1 cut(s) | 345 |
| Bst2UI | CCWGG | 2 cut(s) | 80, 116 |
| Bst4CI | ACNGT | 2 cut(s) | 94, 244 |
| BstC8I | GCNNGC | 1 cut(s) | 446 |
| BstDEI | CTNAG | 1 cut(s) | 440 |
| BstF5I | GGATG | 2 cut(s) | 275, 284 |
| BstH2I | RGCGCY | 1 cut(s) | 215 |
| BstHHI | GCGC | 1 cut(s) | 214 |
| BstKTI | GATC | 2 cut(s) | 197, 469 |
| BstMAI | GTCTC | 2 cut(s) | 452, 483 |
| BstMBI | GATC | 2 cut(s) | 194, 466 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 218 |
| BstNI | CCWGG | 2 cut(s) | 80, 116 |
| BstSCI | CCNGG | 3 cut(s) | 27, 78, 114 |
| BstSFI | CTRYAG | 1 cut(s) | 222 |
| BstV1I | GCAGC | 4 cut(s) | 132, 135, 487, 490 |
| BsuI | GTATCC | 1 cut(s) | 345 |
| BsuRI | GGCC | 2 cut(s) | 85, 448 |
| BtsCI | GGATG | 2 cut(s) | 275, 284 |
| BtsIMutI | CAGTG | 1 cut(s) | 360 |
| BveI | ACCTGC | 1 cut(s) | 163 |
| Cac8I | GCNNGC | 1 cut(s) | 446 |
| CaiI | CAGNNNCTG | 1 cut(s) | 362 |
| CfoI | GCGC | 1 cut(s) | 214 |
| Cfr13I | GGNCC | 3 cut(s) | 83, 378, 428 |
| CviJI | RGCY | 8 cut(s) | 85, 126, 227, 262, 307, 421, 448, 481 |
| CviKI_1 | RGCY | 8 cut(s) | 85, 126, 227, 262, 307, 421, 448, 481 |
| DdeI | CTNAG | 1 cut(s) | 440 |
| DpnI | GATC | 2 cut(s) | 196, 468 |
| DpnII | GATC | 2 cut(s) | 194, 466 |
| Eco47I | GGWCC | 2 cut(s) | 378, 428 |
| Eco47III | AGCGCT | 1 cut(s) | 213 |
| EcoRII | CCWGG | 2 cut(s) | 78, 114 |
| FaiI | YATR | 3 cut(s) | 90, 217, 332 |
| Fnu4HI | GCNGC | 4 cut(s) | 121, 124, 476, 479 |
| FokI | GGATG | 2 cut(s) | 262, 271 |
| Fsp4HI | GCNGC | 4 cut(s) | 121, 124, 476, 479 |
| FspBI | CTAG | 1 cut(s) | 159 |
| GlaI | GCGC | 1 cut(s) | 213 |
| GluI | GCNGC | 4 cut(s) | 121, 124, 476, 479 |
| HaeII | RGCGCY | 1 cut(s) | 215 |
| HaeIII | GGCC | 2 cut(s) | 85, 448 |
| HapII | CCGG | 1 cut(s) | 28 |
| HhaI | GCGC | 1 cut(s) | 214 |
| Hin6I | GCGC | 1 cut(s) | 212 |
| HinP1I | GCGC | 1 cut(s) | 212 |
| HincII | GTYRAC | 1 cut(s) | 352 |
| HindII | GTYRAC | 1 cut(s) | 352 |
| HinfI | GANTC | 1 cut(s) | 293 |
| HpaII | CCGG | 1 cut(s) | 28 |
| Hpy166II | GTNNAC | 1 cut(s) | 352 |
| Hpy188I | TCNGA | 2 cut(s) | 339, 466 |
| Hpy188III | TCNNGA | 2 cut(s) | 178, 198 |
| Hpy8I | GTNNAC | 1 cut(s) | 352 |
| HpyCH4III | ACNGT | 2 cut(s) | 94, 244 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 218 |
| HpyF3I | CTNAG | 1 cut(s) | 440 |
| HspAI | GCGC | 1 cut(s) | 212 |
| Kzo9I | GATC | 2 cut(s) | 194, 466 |
| LmnI | GCTCC | 2 cut(s) | 169, 209 |
| Lsp1109I | GCAGC | 4 cut(s) | 132, 135, 487, 490 |
| MaeI | CTAG | 1 cut(s) | 159 |
| MaeIII | GTNAC | 4 cut(s) | 4, 358, 369, 401 |
| MalI | GATC | 2 cut(s) | 196, 468 |
| MboI | GATC | 2 cut(s) | 194, 466 |
| MboII | GAAGA | 1 cut(s) | 288 |
| MhlI | GDGCHC | 1 cut(s) | 187 |
| MmeI | TCCRAC | 1 cut(s) | 187 |
| MnlI | CCTC | 2 cut(s) | 64, 369 |
| MseI | TTAA | 2 cut(s) | 15, 257 |
| MspI | CCGG | 1 cut(s) | 28 |
| MspR9I | CCNGG | 3 cut(s) | 29, 80, 116 |
| MvaI | CCWGG | 2 cut(s) | 80, 116 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 218 |
| NciI | CCSGG | 1 cut(s) | 29 |
| NdeII | GATC | 2 cut(s) | 194, 466 |
| NlaIV | GGNNCC | 1 cut(s) | 84 |
| NmuCI | GTSAC | 2 cut(s) | 358, 369 |
| PfeI | GAWTC | 1 cut(s) | 293 |
| PflFI | GACNNNGTC | 1 cut(s) | 356 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 362 |
| PkrI | GCNGC | 4 cut(s) | 122, 125, 477, 480 |
| Psp6I | CCWGG | 2 cut(s) | 78, 114 |
| PspGI | CCWGG | 2 cut(s) | 78, 114 |
| PspN4I | GGNNCC | 1 cut(s) | 84 |
| PspPI | GGNCC | 3 cut(s) | 83, 378, 428 |
| PstNI | CAGNNNCTG | 1 cut(s) | 362 |
| PsyI | GACNNNGTC | 1 cut(s) | 356 |
| SaqAI | TTAA | 2 cut(s) | 15, 257 |
| SatI | GCNGC | 4 cut(s) | 121, 124, 476, 479 |
| Sau3AI | GATC | 2 cut(s) | 194, 466 |
| Sau96I | GGNCC | 3 cut(s) | 83, 378, 428 |
| ScrFI | CCNGG | 3 cut(s) | 29, 80, 116 |
| SduI | GDGCHC | 1 cut(s) | 187 |
| SetI | ASST | 6 cut(s) | 128, 177, 207, 229, 257, 380 |
| SfcI | CTRYAG | 1 cut(s) | 222 |
| SinI | GGWCC | 2 cut(s) | 378, 428 |
| SmlI | CTYRAG | 1 cut(s) | 470 |
| SmoI | CTYRAG | 1 cut(s) | 470 |
| SspMI | CTAG | 1 cut(s) | 159 |
| StyD4I | CCNGG | 3 cut(s) | 27, 78, 114 |
| TaaI | ACNGT | 2 cut(s) | 94, 244 |
| TaqI | TCGA | 1 cut(s) | 299 |
| TfiI | GAWTC | 1 cut(s) | 293 |
| Tru1I | TTAA | 2 cut(s) | 15, 257 |
| Tru9I | TTAA | 2 cut(s) | 15, 257 |
| TscAI | CASTG | 1 cut(s) | 367 |
| TseFI | GTSAC | 2 cut(s) | 358, 369 |
| TseI | GCWGC | 4 cut(s) | 120, 123, 475, 478 |
| Tsp45I | GTSAC | 2 cut(s) | 358, 369 |
| TspRI | CASTG | 1 cut(s) | 367 |
| Tth111I | GACNNNGTC | 1 cut(s) | 356 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 362 |
| VpaK11BI | GGWCC | 2 cut(s) | 378, 428 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 363 |
| XspI | CTAG | 1 cut(s) | 159 |