Rorug02G0096000

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000082
Physical Location & Seq
Forward (+)
7851283 .. 7856468
5186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0096000.1

Sequence Viewer

Length: 3483 bp
ATGAAGGCTCCTACAAATGGGTTTTTGGCAACTTCAGTTGAAGGAGAACAGAAGAATATCAATTCAGAGTTATGGCATGCCTGTGCTGGGCCATTGGTTTCTTTGCCTCCAGTTGGAAGTCTTGTGGTTTATTTCCCTCAAGGCCATAGTGAGCAAGTTGCAGCATCTATGCAAAAGGAGACGGATTTCATACCTAACTACCCCAATCTTCCTTCGAAATTGATATGCTTGCTTCACAATGTCACATTACATGCTGATACCGAAACAGATGAGGTGTATGCGCAGATGACTCTCCAACCAGTAAACAAATATGACAAGGAAGCAATACTGGCTTCTGACATGGGCCTCAAGCAAAGCAGGCAACCTTCTGAGTTTTTCTGCAAAACTCTTACAGCTAGTGACACAAGTACTCACGGTGGATTTTCTGTACCTCGTCGTGCAGCTGAGAAGATCTTCCCACCTCTAGATTTCACAATGCAACCACCAGCGCAGGAGCTTGTAGCAAAAGATTTGCATGATAGTGCATGGACATTCAGACATATTTATCGCGGCCAGCCAAAAAGGCACCTGCTGACTACTGGTTGGAGTGTTTTCGTTAGCACAAAAAGACTCTTTGCTGGAGATGCTGTTCTTTTTATAAGAGATGAAAAATCTCAGCTTCTCTTGGGTATAAGGCGTGCTAATAGGCAGCAACCAGCTCTCTCTTCATCAGTCATTTCCAGCGATAGCATGCATATTGGAATTCTTGCAGCTGCAGCTCATGCTGCTGCAAATAACAGTCCATTTACCATATTTTACAACCCGAGGGCCAGCCCTTCTGAGTTTGTGGTACCTTTAGCCAAGTACAATAAAGCGATGTATACCCAAGTTTCACTTGGCATGCGATTCAGAATGATGTTTGAGACTGAAGAGTCAGGAGTTCGCAGATACATGGGTACAATCACTGGGATTAGCGAGTTGGATCATGTTCGTTGGAAAAGTTCTCAATGGCGCAATCTTCAGGTTGGATGGGATGAATCAACGGCTGGTGATCGGCCTAGCCGAGTTTCAATTTGGGAAATTGAGCCTGTTGTAACTCCTTTCTACATCTGCCCACCTCCATTCTTTAGACCAAAGTTTCCCAAACAACCAGGGATGCCAGATGATGAATCTGACATAGAGAATGCTTTCAAGAGAGCCATGCCATGGCTTGGAGATGAGTTTGGCATGAAAAATTCCCCAAGCTCGATTTTCCCTGGTTTGAGTTTAGTGCAGTGGATGAATATGCAACAAAATAATCAGTTTTCAGCCCCTCAGTCTGGATTTTTCCCATCCATGGTTCCTCCAACTACCCTGCATAACGGTACTGATGATCCATCCAAGTTGCTGAATTTTCAAGCTCCTGGTCTAACTGCACCGGGCCAGGTAAATAAAGCAGCTCCACAAAGTCAAGTTAGTCAAGTGCAACAGCCAACTGTGACATGGCCCCAACAGCAGCAGCTGCAGCAACTAATGCAGAATCCTATGAACCAACAGCAGCAAAATCACTCGCAACAGCAACAGCTACAACAATTATTGCATACTCCTTTGAACCAACAACAGCAAAATCATCCCCAACAGCAGCAGCTGCAGCAATTAATGCAAACTCCTGCAAACCAACAGCTGCAAAATTACTCTCAGCAGCAACAGCAGCAACGGGAGCAACAAAACCATCAAGAACCTCAAGGCCTTCAACAGCTGCAGCAACTTCATCAGCTACATCAGCAGCAACCACAACGGCAACAGTCACAGCAACAACAACAGTTAGGTCAACCGACTCTTGTAAGTAATGGTCTTGTTACTCCGAACCAAATCCCAAGCCAAAATTCGCAGCAACCAATGATGTTCACTCAGCTTCAGCAGCAGCATTCACTAACAAGCAGTACCCAATCCCAGCAAGCAGTCCACACTCCTAATAAGAATTCATTCCAGTTGGCAGCCGGCACACAAGACTCACAAATTCAGCAACAATTGGAACCGCAACCAAGCCTGTTGCAAAGGCAGCAGCAGGTACAGTTGCAACAGTCCCCATTGCAGTTGTTGCAACAGCCACAGAAAGCACAGCAGCAGCCACAAGTACAATCATCGTCACAACAGGCTTTGTCTGAGCAACAACTTCACTTACAGTTGCTTCAGAAATTGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAACAACAACAATTATTCTCTCCATCAAGCCCACTTTTGCAGCCGCAAATGCTACAGCAGCAGCTGGCCCATCAACAAAACCAGCAATTACAACAGTTGCAGTTGCCTCTGTCTCAACATCATCAGCAACAGCTAAGCGGTAACAGCTTCTCAGCAGACAAACTTCTCAACAGCAACAACTTCTCGGCTTCACCAATGATGCAGGCCCAACATGTTTCATCTATCCAACAACAGAACCAGCACAAGCCAGTTACAGCAATCAGAAGCCATTCCAATCTTACAGAAGGGGACGGTCCATCATGTTCAACCTCCCCTTCTACAAATAATTGCCAGGTGTCCTCATCAAACCTACTGAACAGGAATCAGCAAGGAACAGCCATGTTGATAGGGGATCCAGTGGCGGAGCCTTCTAGTAACTTGGTTCATGATTTTCAGAGCAAGTCTGATATTCGAATCAAACATGAGTTGCCCATTTCAAAAGGACTGGACCAAATTAAATATAAAGGTGCCATCACTGATCAGTTGGAAGCTTCCTCTTCTGGAACTTCGTACTGTCTGGATGCTAGCACAATACAGCAGAACTACGCGCTCCCATCCTTCTGTTTAGATGGTGATGTCCAATCACATCCTCGGAACAATCTTCCGTTTTCAGCTAATATTGATGGATTGGCACCTGACACTTTGTTGTCAAGAGGATATGACTCTCAGAAAGATCTTCAGAACTTACTGTCTAATTATGGTGGGACACCAAGAGATATTGAGACAGAGCTATCTACTGCTGCAATCAGCTCTCAATCATTTGGGGCGCCAAACATGCCATTCAAGCCTGGGTGCTCTAGTGATGTTGCTCTAAATGATGCTGGAGTTCTAAATAATGGTTTGTGGGGATCACAGGCCCAACGTATGAGAACGTATACAAAGGTTCAAAAGCGTGGATCAGTGGGAAGATGTATTGATGTTACCCGGTATAAAGGCTATGATGAGCTCAGGCATGATCTAGCCCGCATGTTTGGGATTGAAGGGCAACTAGAAGATCCACAAAGAACTGACTGGAAGCTTGTTTATGTGGATCACGAAAATGACATACTTCTTGTTGGTGATGATCCATGGGAGGAGTTCGTGAGCTGTGTCCAGAGTATAAAGATACTGTCATCTGTTGAAGTACAACAAATGAGCTTGGATGGAGATCTGGGAAATGTTCCTGTTCCCAACCAAGCTTGCAGTGGAACTGACAGTGGAAATGCATGGAGGGCACCATATGAAGATAACTCTGCAGCCTCATTCAATCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

1160

Amino Acids

129.82

Weight (kDa)

6.2

Isoelectric Point (pI)

69.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 126 - 227 3.2e-20 B3 DNA binding domain
ARF_AD PF06507 275 - 357 6.7e-35 Auxin response factor ancillary domain
AUX_IAA PF02309 1030 - 1119 1.7e-08 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000082)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17540 FvH4_1g17540 FvH4_1g17540 FvH4_1g17540 FvH4_1g17550 FvH4_1g17560 FvH4_1g17570 FvH4_1g17570 FvH4_1g17570 FvH4_1g17581 FvH4_1g18380 FvH4_1g18391 FvH4_1g18430 FvH4_1g18470 FvH4_1g18480 FvH4_1g18480 FvH4_1g18480 FvH4_1g18870 FvH4_2g36701 FvH4_2g36701 FvH4_2g36710 FvH4_2g36711 FvH4_3g01100 FvH4_3g01100 FvH4_3g14940 FvH4_3g14941 FvH4_3g14950 FvH4_3g14952 FvH4_3g14954 FvH4_3g14954 FvH4_3g14954 FvH4_3g15782 FvH4_3g32340 FvH4_3g32350 FvH4_3g32390 FvH4_3g32390 FvH4_3g44560 FvH4_3g44581 FvH4_3g44650 FvH4_3g44660 FvH4_3g44671 FvH4_3g45110 FvH4_3g45120 FvH4_3g45161 FvH4_4g30361 FvH4_6g14790 FvH4_6g14900 FvH4_6g14900 FvH4_6g14900 FvH4_6g14900 FvH4_6g14900 FvH4_6g14972 FvH4_6g14980 FvH4_6g14980 FvH4_6g14981 FvH4_6g14990 FvH4_6g14991 FvH4_6g14991 FvH4_6g14993 FvH4_6g14993 FvH4_6g14994 FvH4_6g15010 FvH4_6g19961
malus_domestica MD09G1138900.v1.1
prunus_persica Prupe.6G214500_v2.0.a1 Prupe.6G214600_v2.0.a1 Prupe.6G214700_v2.0.a1 Prupe.6G214900_v2.0.a1 Prupe.6G215100_v2.0.a1 Prupe.7G008100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321661 RchiOBHm_Chr1g0378141 RchiOBHm_Chr2g0101031 RchiOBHm_Chr2g0107781 RchiOBHm_Chr2g0107801 RchiOBHm_Chr2g0107811 RchiOBHm_Chr2g0107821 RchiOBHm_Chr2g0107841 RchiOBHm_Chr2g0107851 RchiOBHm_Chr2g0107881 RchiOBHm_Chr2g0107891 RchiOBHm_Chr2g0109731 RchiOBHm_Chr2g0109761 RchiOBHm_Chr2g0109771 RchiOBHm_Chr2g0109781 RchiOBHm_Chr3g0468171 RchiOBHm_Chr3g0468191 RchiOBHm_Chr3g0475411 RchiOBHm_Chr5g0007501 RchiOBHm_Chr5g0024531 RchiOBHm_Chr5g0053901 RchiOBHm_Chr5g0059111 RchiOBHm_Chr5g0059201 RchiOBHm_Chr5g0059211 RchiOBHm_Chr5g0059291 RchiOBHm_Chr5g0059301 RchiOBHm_Chr5g0059321 RchiOBHm_Chr5g0059551 RchiOBHm_Chr5g0081341 RchiOBHm_Chr7g0211081
rosa_laevigata RLG00000003023 RLG00000017039 RLG00000017135 RLG00000017616 RLG00000017620 RLG00000017621 RLG00000017622 RLG00000017623 RLG00000017624 RLG00000017625 RLG00000017762 RLG00000017763 RLG00000017764 RLG00000017765 RLG00000024443 RLG00000026103 RLG00000031462 RLG00000035283 RLG00000036923 RLG00000036924 RLG00000036925
rosa_multiflora Rmu_co7981142.1_g000001 Rmu_co8037462.1_g000001 Rmu_co8043938.1_g000001 Rmu_co8085092.1_g000001 Rmu_co8243479.1_g000002 Rmu_co8254857.1_g000001 Rmu_co8270991.1_g000001 Rmu_co8304467.1_g000001 Rmu_co8322247.1_g000001 Rmu_sc0000186.1_g000008 Rmu_sc0000375.1_g000022 Rmu_sc0000625.1_g000006 Rmu_sc0001465.1_g000001 Rmu_sc0001484.1_g000034 Rmu_sc0001484.1_g000035 Rmu_sc0001484.1_g000036 Rmu_sc0001484.1_g000038 Rmu_sc0001484.1_g000041 Rmu_sc0001484.1_g000042 Rmu_sc0001793.1_g000054 Rmu_sc0001810.1_g000002 Rmu_sc0001810.1_g000003 Rmu_sc0001810.1_g000004 Rmu_sc0001862.1_g000003 Rmu_sc0002076.1_g000008 Rmu_sc0002076.1_g000009 Rmu_sc0002983.1_g000017 Rmu_sc0002983.1_g000035 Rmu_sc0002983.1_g000038 Rmu_sc0003121.1_g000002 Rmu_sc0003121.1_g000005 Rmu_sc0003121.1_g000006 Rmu_sc0003121.1_g000007 Rmu_sc0003659.1_g000007 Rmu_sc0004524.1_g000009 Rmu_sc0004524.1_g000010 Rmu_sc0004524.1_g000012 Rmu_sc0004650.1_g000003 Rmu_sc0006215.1_g000003 Rmu_sc0006889.1_g000020 Rmu_sc0006889.1_g000030 Rmu_sc0006959.1_g000021 Rmu_sc0007173.1_g000008 Rmu_sc0007698.1_g000019 Rmu_sc0007768.1_g000009 Rmu_sc0008992.1_g000002 Rmu_sc0008992.1_g000004 Rmu_sc0009575.1_g000001 Rmu_sc0009575.1_g000008 Rmu_sc0009650.1_g000002 Rmu_sc0009650.1_g000005 Rmu_sc0009650.1_g000009 Rmu_sc0012421.1_g000005 Rmu_sc0012471.1_g000023 Rmu_sc0012471.1_g000025 Rmu_sc0012670.1_g000002 Rmu_sc0012670.1_g000003 Rmu_sc0016637.1_g000005 Rmu_sc0018174.1_g000002 Rmu_sc0031424.1_g000001 Rmu_ssc0000182.1_g000007 Rmu_ssc0000182.1_g000010 Rmu_ssc0000432.1_g000029 Rmu_ssc0000432.1_g000035 Rmu_ssc0000432.1_g000036 Rmu_ssc0000432.1_g000042
rosa_roxburghii Rroxscaffold_153G00436690 Rroxscaffold_1G00002200 Rroxscaffold_1G00002220 Rroxscaffold_1G00021160 Rroxscaffold_1G00021240 Rroxscaffold_1G00021310 Rroxscaffold_1G00025950 Rroxscaffold_1G00026710 Rroxscaffold_1G00054720 Rroxscaffold_2G00104770 Rroxscaffold_2G00104780 Rroxscaffold_2G00133820 Rroxscaffold_2G00133950 Rroxscaffold_2G00133970 Rroxscaffold_2G00133980 Rroxscaffold_2G00133990 Rroxscaffold_2G00135690 Rroxscaffold_2G00135820 Rroxscaffold_2G00135850 Rroxscaffold_2G00135860 Rroxscaffold_2G00135890 Rroxscaffold_2G00135910 Rroxscaffold_2G00135920 Rroxscaffold_2G00135930 Rroxscaffold_2G00135940 Rroxscaffold_2G00141620 Rroxscaffold_3G00248120 Rroxscaffold_4G00277310 Rroxscaffold_6G00406330 Rroxscaffold_6G00412930 Rroxscaffold_6G00412940 Rroxscaffold_6G00412950 Rroxscaffold_6G00412970 Rroxscaffold_6G00413290
rosa_rugosa Rorug01G0019000 Rorug02G0095500 Rorug02G0095600 Rorug02G0095700 Rorug02G0095800 Rorug02G0095900 Rorug02G0096000 Rorug02G0096100 Rorug02G0096200 Rorug02G0147500 Rorug02G0147600 Rorug02G0147700 Rorug02G0147800 Rorug02G0148100 Rorug02G0148300 Rorug02G0148400 Rorug02G0148500 Rorug02G0148600 Rorug02G0149800 Rorug02G0162500 Rorug02G0162600 Rorug02G0162700 Rorug02G0162800 Rorug02G0510300 Rorug03G0090600 Rorug03G0093700 Rorug03G0093800 Rorug03G0093900 Rorug03G0094000 Rorug03G0148600 Rorug04G0427700 Rorug05G0083700 Rorug05G0321200 Rorug05G0321300 Rorug05G0321400 Rorug05G0321500 Rorug05G0321600 Rorug05G0321900 Rorug05G0322000 Rorug05G0322000 Rorug05G0322300 Rorug05G0475400 Rorug05G0475500 Rorug05G0475600.1 Rorug05G0475700 Rorug05G0475900
rosa_samantha Rh1DG457400 Rh2AG197800 Rh2AG208800 Rh5AG386200 Rh5BG397600 Rh5DG174500 Rh5DG381100 Rh5DG414700 Rh5DG414800 Rh5DG415500 Rh5DG415800
rosa_wichuraiana Rw0G010830 Rw0G010840 Rw0G010870 Rw0G015860 Rw0G015870 Rw1G037410 Rw1G040530 Rw2G011200 Rw2G011230 Rw2G015590 Rw2G015600 Rw2G015610 Rw2G015630 Rw2G015640 Rw2G015660 Rw2G015680 Rw2G015690 Rw3G013400 Rw3G013410 Rw3G018040 Rw5G036340 Rw5G036390 Rw5G049010 Rw5G049020 Rw5G049030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
AarI CACCTGC 1 cut(s) 576
AasI GACNNNNNNGTC 1 cut(s) 910
Acc16I TGCGCA 1 cut(s) 282
Acc36I ACCTGC 2 cut(s) 576, 2017
Acc65I GGTACC 1 cut(s) 829
AccB1I GGYRCC 6 cut(s) 564, 829, 2696, 2860, 2995, 3442
AccB7I CCANNNNNTGG 2 cut(s) 1185, 1190
AccI GTMKAC 2 cut(s) 860, 3104
AccII CGCG 2 cut(s) 549, 2777
AciI CCGC 6 cut(s) 549, 1997, 2234, 2328, 2591, 3193
AclWI GGATC 9 cut(s) 969, 1346, 2576, 2589, 3085, 3133, 3218, 3267, 3287
AcoI YGGCCR 1 cut(s) 550
AcsI RAATTY 6 cut(s) 741, 1213, 1369, 1843, 1939, 1977
AcuI CTGAAG 6 cut(s) 18, 927, 983, 1859, 2135, 2891
AcyI GRCGYC 1 cut(s) 2996
AfiI CCNNNNNNNGG 7 cut(s) 17, 87, 113, 1185, 1190, 1298, 1315
AflIII ACRYGT 1 cut(s) 2401
AjnI CCWGG 6 cut(s) 1129, 1234, 1381, 1401, 2520, 3016
AjuI GAANNNNNNNTTGG 2 cut(s) 1828, 1860
AloI GAACNNNNNNTCC 2 cut(s) 612, 644
Alw21I GWGCWC 2 cut(s) 3026, 3177
Alw26I GTCTC 4 cut(s) 173, 896, 2307, 2945
AlwI GGATC 9 cut(s) 969, 1346, 2576, 2589, 3085, 3133, 3218, 3267, 3287
AlwNI CAGNNNCTG 3 cut(s) 1480, 1606, 2254
Ama87I CYCGRG 1 cut(s) 802
ApoI RAATTY 6 cut(s) 741, 1213, 1369, 1843, 1939, 1977
AseI ATTAAT 1 cut(s) 1616
Asp700I GAANNNNTTC 4 cut(s) 452, 1166, 1943, 2458
Asp718I GGTACC 1 cut(s) 829
AspLEI GCGC 5 cut(s) 283, 490, 993, 2779, 2998
AsuC2I CCSGG 2 cut(s) 1398, 3154
AsuHPI GGTGA 4 cut(s) 1040, 2373, 2813, 3299
AsuII TTCGAA 2 cut(s) 215, 2641
AsuNHI GCTAGC 1 cut(s) 2753
AvaI CYCGRG 1 cut(s) 802
AvaII GGWCC 2 cut(s) 2483, 2677
BaeGI GKGCMC 1 cut(s) 3445
BaeI ACNNNNGTAYC 2 cut(s) 919, 952
BamHI GGATCC 1 cut(s) 2581
BanI GGYRCC 6 cut(s) 564, 829, 2696, 2860, 2995, 3442
BanII GRGCYC 1 cut(s) 3177
Bbv12I GWGCWC 2 cut(s) 3026, 3177
BceAI ACGGC 2 cut(s) 1038, 1772
BciT130I CCWGG 6 cut(s) 1131, 1236, 1383, 1403, 2522, 3018
BclI TGATCA 1 cut(s) 2707
BcnI CCSGG 2 cut(s) 1398, 3154
BcoDI GTCTC 4 cut(s) 173, 896, 2307, 2945
BfaI CTAG 8 cut(s) 396, 464, 1038, 2601, 2754, 3027, 3188, 3218
BfmI CTRYAG 6 cut(s) 753, 1481, 1607, 1718, 2243, 3462
BfoI RGCGCY 1 cut(s) 2999
BfuAI ACCTGC 2 cut(s) 576, 2017
BglI GCCNNNNNGGC 1 cut(s) 562
BglII AGATCT 3 cut(s) 450, 2902, 3376
BlpI GCTNAGC 1 cut(s) 2324
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 8 cut(s) 1131, 1236, 1383, 1398, 1403, 2522, 3018, 3154
Bme18I GGWCC 2 cut(s) 2483, 2677
BmeT110I CYCGRG 1 cut(s) 802
BmrFI CCNGG 8 cut(s) 1131, 1236, 1383, 1398, 1403, 2522, 3018, 3154
BmrI ACTGGG 1 cut(s) 954
BmsI GCATC 6 cut(s) 173, 613, 1125, 2379, 2740, 3037
BmtI GCTAGC 1 cut(s) 2757
BmuI ACTGGG 1 cut(s) 954
BpmI CTGGAG 3 cut(s) 93, 639, 3072
Bpu10I CCTNAGC 1 cut(s) 3176
Bpu1102I GCTNAGC 1 cut(s) 2324
Bpu14I TTCGAA 2 cut(s) 215, 2641
BpuEI CTTGAG 3 cut(s) 123, 332, 1686
BpuMI CCSGG 2 cut(s) 1398, 3154
BsaBI GATNNNNATC 1 cut(s) 3375
BsaHI GRCGYC 1 cut(s) 2996
BsaJI CCNNGG 8 cut(s) 803, 1130, 1184, 1234, 1314, 2819, 3017, 3296
BsaXI ACNNNNNCTCC 2 cut(s) 612, 642
Bsc4I CCNNNNNNNGG 7 cut(s) 17, 87, 113, 1185, 1190, 1298, 1315
Bse118I RCCGGY 1 cut(s) 1958
Bse3DI GCAATG 1 cut(s) 2048
Bse8I GATNNNNATC 1 cut(s) 3375
BseBI CCWGG 6 cut(s) 1131, 1236, 1383, 1403, 2522, 3018
BseDI CCNNGG 8 cut(s) 803, 1130, 1184, 1234, 1314, 2819, 3017, 3296
BseJI GATNNNNATC 1 cut(s) 3375
BseLI CCNNNNNNNGG 7 cut(s) 17, 87, 113, 1185, 1190, 1298, 1315
BseMI GCAATG 1 cut(s) 2048
BseRI GAGGAG 1 cut(s) 3317
BseSI GKGCMC 1 cut(s) 3445
BseYI CCCAGC 2 cut(s) 86, 1911
BsgI GTGCAG 3 cut(s) 459, 1271, 1377
Bsh1236I CGCG 2 cut(s) 549, 2777
BshNI GGYRCC 6 cut(s) 564, 829, 2696, 2860, 2995, 3442
BsiHKAI GWGCWC 2 cut(s) 3026, 3177
BsiHKCI CYCGRG 1 cut(s) 802
BsiSI CCGG 3 cut(s) 1397, 1959, 3154
BslFI GGGAC 3 cut(s) 2029, 2492, 2947
BslI CCNNNNNNNGG 7 cut(s) 17, 87, 113, 1185, 1190, 1298, 1315
BsmAI GTCTC 4 cut(s) 173, 896, 2307, 2945
BsmBI CGTCTC 1 cut(s) 173
BsmFI GGGAC 3 cut(s) 2029, 2492, 2947
BsmI GAATGC 2 cut(s) 1168, 1885
BsoBI CYCGRG 1 cut(s) 802
Bsp119I TTCGAA 2 cut(s) 215, 2641
Bsp1286I GDGCHC 3 cut(s) 3026, 3177, 3445
Bsp1720I GCTNAGC 1 cut(s) 2324
Bsp19I CCATGG 3 cut(s) 1184, 1314, 3296
BspACI CCGC 6 cut(s) 549, 1997, 2234, 2328, 2591, 3193
BspFNI CGCG 2 cut(s) 549, 2777
BspHI TCATGA 1 cut(s) 2614
BspMAI CTGCAG 5 cut(s) 757, 1485, 1611, 1722, 3466
BspMI ACCTGC 2 cut(s) 576, 2017
BspOI GCTAGC 1 cut(s) 2757
BspPI GGATC 9 cut(s) 969, 1346, 2576, 2589, 3085, 3133, 3218, 3267, 3287
BspT104I TTCGAA 2 cut(s) 215, 2641
BspT107I GGYRCC 6 cut(s) 564, 829, 2696, 2860, 2995, 3442
BsrDI GCAATG 1 cut(s) 2048
BsrFI RCCGGY 1 cut(s) 1958
BssAI RCCGGY 1 cut(s) 1958
BssECI CCNNGG 8 cut(s) 803, 1130, 1184, 1234, 1314, 2819, 3017, 3296
BssNAI GTATAC 2 cut(s) 861, 3105
BssNI GRCGYC 1 cut(s) 2996
BssT1I CCWWGG 3 cut(s) 1184, 1314, 3296
Bst1107I GTATAC 2 cut(s) 861, 3105
Bst2UI CCWGG 6 cut(s) 1131, 1236, 1383, 1403, 2522, 3018
Bst6I CTCTTC 3 cut(s) 709, 903, 2731
BstACI GRCGYC 1 cut(s) 2996
BstAPI GCANNNNNTGC 6 cut(s) 761, 1480, 1492, 1606, 1618, 2059
BstBI TTCGAA 2 cut(s) 215, 2641
BstDSI CCRYGG 3 cut(s) 1184, 1314, 3296
BstFNI CGCG 2 cut(s) 549, 2777
BstH2I RGCGCY 1 cut(s) 2999
BstHHI GCGC 5 cut(s) 283, 490, 993, 2779, 2998
BstMAI GTCTC 4 cut(s) 173, 896, 2307, 2945
BstNI CCWGG 6 cut(s) 1131, 1236, 1383, 1403, 2522, 3018
BstNSI RCATGY 7 cut(s) 80, 254, 733, 883, 2405, 3007, 3199
BstSCI CCNGG 8 cut(s) 1129, 1234, 1381, 1396, 1401, 2520, 3016, 3152
BstSFI CTRYAG 6 cut(s) 753, 1481, 1607, 1718, 2243, 3462
BstSLI GKGCMC 1 cut(s) 3445
BstUI CGCG 2 cut(s) 549, 2777
BstX2I RGATCY 5 cut(s) 450, 2581, 2902, 3223, 3376
BstYI RGATCY 5 cut(s) 450, 2581, 2902, 3223, 3376
BstZ17I GTATAC 2 cut(s) 861, 3105
BtgI CCRYGG 3 cut(s) 1184, 1314, 3296
BtgZI GCGATG 1 cut(s) 869
BtsI GCAGTG 2 cut(s) 1259, 3418
BtsIMutI CAGTG 7 cut(s) 942, 1259, 2592, 2703, 3135, 3418, 3430
BveI ACCTGC 2 cut(s) 576, 2017
CaiI CAGNNNCTG 3 cut(s) 1480, 1606, 2254
CciI TCATGA 1 cut(s) 2614
CfoI GCGC 5 cut(s) 283, 490, 993, 2779, 2998
Cfr10I RCCGGY 1 cut(s) 1958
DinI GGCGCC 1 cut(s) 2997
DrdI GACNNNNNNGTC 1 cut(s) 910
DseDI GACNNNNNNGTC 1 cut(s) 910
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 3 cut(s) 709, 903, 2731
EarI CTCTTC 3 cut(s) 709, 903, 2731
EciI GGCGGA 1 cut(s) 2606
Ecl136II GAGCTC 1 cut(s) 3175
Eco130I CCWWGG 3 cut(s) 1184, 1314, 3296
Eco147I AGGCCT 1 cut(s) 1707
Eco24I GRGCYC 1 cut(s) 3177
Eco47I GGWCC 2 cut(s) 2483, 2677
Eco53kI GAGCTC 1 cut(s) 3175
Eco57I CTGAAG 6 cut(s) 18, 927, 983, 1859, 2135, 2891
Eco88I CYCGRG 1 cut(s) 802
EcoICRI GAGCTC 1 cut(s) 3175
EcoRI GAATTC 2 cut(s) 741, 1939
EcoRII CCWGG 6 cut(s) 1129, 1234, 1381, 1401, 2520, 3016
EcoT14I CCWWGG 3 cut(s) 1184, 1314, 3296
EcoT22I ATGCAT 2 cut(s) 735, 3436
EcoT38I GRGCYC 1 cut(s) 3177
EgeI GGCGCC 1 cut(s) 2997
EheI GGCGCC 1 cut(s) 2997
ErhI CCWWGG 3 cut(s) 1184, 1314, 3296
Esp3I CGTCTC 1 cut(s) 173
FalI AAGNNNNNCTT 2 cut(s) 858, 890
FaqI GGGAC 3 cut(s) 2029, 2492, 2947
FauI CCCGC 1 cut(s) 3200
FauNDI CATATG 1 cut(s) 3448
FbaI TGATCA 1 cut(s) 2707
FblI GTMKAC 2 cut(s) 860, 3104
FriOI GRGCYC 1 cut(s) 3177
FspBI CTAG 8 cut(s) 396, 464, 1038, 2601, 2754, 3027, 3188, 3218
FspI TGCGCA 1 cut(s) 282
GlaI GCGC 5 cut(s) 282, 489, 992, 2778, 2997
GsaI CCCAGC 2 cut(s) 90, 1915
GsuI CTGGAG 3 cut(s) 93, 639, 3072
HaeII RGCGCY 1 cut(s) 2999
HapII CCGG 3 cut(s) 1397, 1959, 3154
HhaI GCGC 5 cut(s) 283, 490, 993, 2779, 2998
Hin1I GRCGYC 1 cut(s) 2996
Hin6I GCGC 5 cut(s) 281, 488, 991, 2777, 2996
HinP1I GCGC 5 cut(s) 281, 488, 991, 2777, 2996
HincII GTYRAC 1 cut(s) 1790
HindII GTYRAC 1 cut(s) 1790
HindIII AAGCTT 3 cut(s) 2718, 3245, 3405
HpaII CCGG 3 cut(s) 1397, 1959, 3154
HphI GGTGA 4 cut(s) 1040, 2373, 2813, 3299
Hpy166II GTNNAC 6 cut(s) 304, 861, 1790, 1866, 1924, 3105
Hpy8I GTNNAC 6 cut(s) 304, 861, 1790, 1866, 1924, 3105
Hpy99I CGWCG 1 cut(s) 438
HpyCH4IV ACGT 2 cut(s) 3091, 3101
HpySE526I ACGT 2 cut(s) 3091, 3101
Hsp92I GRCGYC 1 cut(s) 2996
HspAI GCGC 5 cut(s) 281, 488, 991, 2777, 2996
KasI GGCGCC 1 cut(s) 2995
KpnI GGTACC 1 cut(s) 833
KroI GCCGGC 1 cut(s) 1958
KroNI GCCGGC 1 cut(s) 1960
Ksp22I TGATCA 1 cut(s) 2707
LmnI GCTCC 7 cut(s) 13, 493, 1384, 1423, 1678, 2593, 2784
LweI GCATC 6 cut(s) 173, 613, 1125, 2379, 2740, 3037
MaeI CTAG 8 cut(s) 396, 464, 1038, 2601, 2754, 3027, 3188, 3218
MaeII ACGT 2 cut(s) 3091, 3101
MfeI CAATTG 1 cut(s) 1988
MflI RGATCY 5 cut(s) 450, 2581, 2902, 3223, 3376
MhlI GDGCHC 3 cut(s) 3026, 3177, 3445
Mly113I GGCGCC 1 cut(s) 2996
MlyI GAGTC 6 cut(s) 283, 603, 920, 1789, 1964, 2885
MmeI TCCRAC 9 cut(s) 94, 319, 563, 939, 953, 985, 1349, 2440, 2694
Mph1103I ATGCAT 2 cut(s) 735, 3436
MroNI GCCGGC 1 cut(s) 1958
MroXI GAANNNNTTC 4 cut(s) 452, 1166, 1943, 2458
MseI TTAA 2 cut(s) 1616, 2685
MslI CAYNNNNRTG 3 cut(s) 81, 519, 3267
MspA1I CMGCKG 7 cut(s) 443, 752, 1480, 1606, 1642, 1717, 2254
MspI CCGG 3 cut(s) 1397, 1959, 3154
MspR9I CCNGG 8 cut(s) 1131, 1236, 1383, 1398, 1403, 2522, 3018, 3154
MunI CAATTG 1 cut(s) 1988
Mva1269I GAATGC 2 cut(s) 1168, 1885
MvaI CCWGG 6 cut(s) 1131, 1236, 1383, 1403, 2522, 3018
MvnI CGCG 2 cut(s) 549, 2777
NaeI GCCGGC 1 cut(s) 1960
NarI GGCGCC 1 cut(s) 2996
NciI CCSGG 2 cut(s) 1398, 3154
NcoI CCATGG 3 cut(s) 1184, 1314, 3296
NdeI CATATG 1 cut(s) 3448
NgoMIV GCCGGC 1 cut(s) 1958
NheI GCTAGC 1 cut(s) 2753
NmeAIII GCCGAG 2 cut(s) 1067, 2354
NmuCI GTSAC 5 cut(s) 241, 398, 1456, 1764, 2106
NsbI TGCGCA 1 cut(s) 282
NsiI ATGCAT 2 cut(s) 735, 3436
NspI RCATGY 7 cut(s) 80, 254, 733, 883, 2405, 3007, 3199
NspV TTCGAA 2 cut(s) 215, 2641
PaeI GCATGC 3 cut(s) 80, 733, 883
PagI TCATGA 1 cut(s) 2614
PaqCI CACCTGC 1 cut(s) 576
PceI AGGCCT 1 cut(s) 1707
PciI ACATGT 1 cut(s) 2401
PcsI WCGNNNNNNNCGW 1 cut(s) 1039
PctI GAATGC 2 cut(s) 1168, 1885
PdiI GCCGGC 1 cut(s) 1960
PdmI GAANNNNTTC 4 cut(s) 452, 1166, 1943, 2458
PfeI GAWTC 6 cut(s) 885, 1016, 1148, 1498, 2551, 2643
PflMI CCANNNNNTGG 2 cut(s) 1185, 1190
PleI GAGTC 6 cut(s) 283, 603, 919, 1789, 1964, 2885
PluTI GGCGCC 1 cut(s) 2999
PpsI GAGTC 6 cut(s) 283, 603, 919, 1789, 1964, 2885
PscI ACATGT 1 cut(s) 2401
PshBI ATTAAT 1 cut(s) 1616
PsiI TTATAA 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 3177
Psp6I CCWGG 6 cut(s) 1129, 1234, 1381, 1401, 2520, 3016
PspFI CCCAGC 2 cut(s) 86, 1911
PspGI CCWGG 6 cut(s) 1129, 1234, 1381, 1401, 2520, 3016
PstI CTGCAG 5 cut(s) 757, 1485, 1611, 1722, 3466
PstNI CAGNNNCTG 3 cut(s) 1480, 1606, 2254
PsuI RGATCY 5 cut(s) 450, 2581, 2902, 3223, 3376
PvuII CAGCTG 7 cut(s) 443, 752, 1480, 1606, 1642, 1717, 2254
RseI CAYNNNNRTG 3 cut(s) 81, 519, 3267
SacI GAGCTC 1 cut(s) 3177
SaqAI TTAA 2 cut(s) 1616, 2685
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 6 cut(s) 283, 603, 920, 1789, 1964, 2885
ScrFI CCNGG 8 cut(s) 1131, 1236, 1383, 1398, 1403, 2522, 3018, 3154
SduI GDGCHC 3 cut(s) 3026, 3177, 3445
SfaNI GCATC 6 cut(s) 173, 613, 1125, 2379, 2740, 3037
SfcI CTRYAG 6 cut(s) 753, 1481, 1607, 1718, 2243, 3462
SfoI GGCGCC 1 cut(s) 2997
SfuI TTCGAA 2 cut(s) 215, 2641
SinI GGWCC 2 cut(s) 2483, 2677
SmiMI CAYNNNNRTG 3 cut(s) 81, 519, 3267
SmlI CTYRAG 3 cut(s) 138, 347, 1701
SmoI CTYRAG 3 cut(s) 138, 347, 1701
SphI GCATGC 3 cut(s) 80, 733, 883
SseBI AGGCCT 1 cut(s) 1707
SsiI CCGC 6 cut(s) 549, 1997, 2234, 2328, 2591, 3193
SspDI GGCGCC 1 cut(s) 2995
SspI AATATT 1 cut(s) 2848
SspMI CTAG 8 cut(s) 396, 464, 1038, 2601, 2754, 3027, 3188, 3218
SstI GAGCTC 1 cut(s) 3177
StuI AGGCCT 1 cut(s) 1707
StyD4I CCNGG 8 cut(s) 1129, 1234, 1381, 1396, 1401, 2520, 3016, 3152
StyI CCWWGG 3 cut(s) 1184, 1314, 3296
TaiI ACGT 2 cut(s) 3094, 3104
TaqI TCGA 3 cut(s) 215, 1226, 2641
TatI WGTACW 4 cut(s) 407, 843, 2095, 3352
TauI GCSGC 2 cut(s) 552, 2236
TfiI GAWTC 6 cut(s) 885, 1016, 1148, 1498, 2551, 2643
Tru1I TTAA 2 cut(s) 1616, 2685
Tru9I TTAA 2 cut(s) 1616, 2685
TscAI CASTG 7 cut(s) 949, 1259, 2592, 2710, 3135, 3418, 3430
TseFI GTSAC 5 cut(s) 241, 398, 1456, 1764, 2106
Tsp45I GTSAC 5 cut(s) 241, 398, 1456, 1764, 2106
TspGWI ACGGA 2 cut(s) 197, 2823
TspRI CASTG 7 cut(s) 949, 1259, 2592, 2710, 3135, 3418, 3430
Van91I CCANNNNNTGG 2 cut(s) 1185, 1190
VpaK11BI GGWCC 2 cut(s) 2483, 2677
VspI ATTAAT 1 cut(s) 1616
XapI RAATTY 6 cut(s) 741, 1213, 1369, 1843, 1939, 1977
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 7 cut(s) 80, 254, 733, 883, 2405, 3007, 3199
XcmI CCANNNNNNNNNTGG 3 cut(s) 872, 1458, 3410
XmiI GTMKAC 2 cut(s) 860, 3104
XmnI GAANNNNTTC 4 cut(s) 452, 1166, 1943, 2458
XspI CTAG 8 cut(s) 396, 464, 1038, 2601, 2754, 3027, 3188, 3218
ZrmI AGTACT 1 cut(s) 409
Zsp2I ATGCAT 2 cut(s) 735, 3436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.