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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 51 |
| AciI | CCGC | 1 cut(s) | 191 |
| AclWI | GGATC | 1 cut(s) | 320 |
| AcoI | YGGCCR | 2 cut(s) | 81, 141 |
| AcsI | RAATTY | 1 cut(s) | 28 |
| AgsI | TTSAA | 2 cut(s) | 107, 116 |
| AjnI | CCWGG | 1 cut(s) | 138 |
| AluBI | AGCT | 1 cut(s) | 46 |
| AluI | AGCT | 1 cut(s) | 46 |
| AlwI | GGATC | 1 cut(s) | 320 |
| AoxI | GGCC | 2 cut(s) | 81, 141 |
| ApoI | RAATTY | 1 cut(s) | 28 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 197, 229 |
| AsuHPI | GGTGA | 1 cut(s) | 49 |
| BaeGI | GKGCMC | 1 cut(s) | 56 |
| BalI | TGGCCA | 1 cut(s) | 143 |
| BanI | GGYRCC | 1 cut(s) | 51 |
| BbsI | GAAGAC | 1 cut(s) | 141 |
| BciT130I | CCWGG | 1 cut(s) | 140 |
| BfaI | CTAG | 2 cut(s) | 230, 239 |
| BisI | GCNGC | 1 cut(s) | 191 |
| BlsI | GCNGC | 1 cut(s) | 192 |
| Bme1390I | CCNGG | 1 cut(s) | 140 |
| BmiI | GGNNCC | 2 cut(s) | 53, 259 |
| BmrFI | CCNGG | 1 cut(s) | 140 |
| BmsI | GCATC | 1 cut(s) | 235 |
| BpiI | GAAGAC | 1 cut(s) | 141 |
| Bpu10I | CCTNAGC | 1 cut(s) | 47 |
| Bsa29I | ATCGAT | 1 cut(s) | 64 |
| BsaJI | CCNNGG | 1 cut(s) | 121 |
| Bse3DI | GCAATG | 1 cut(s) | 150 |
| BseBI | CCWGG | 1 cut(s) | 140 |
| BseCI | ATCGAT | 1 cut(s) | 64 |
| BseDI | CCNNGG | 1 cut(s) | 121 |
| BseMI | GCAATG | 1 cut(s) | 150 |
| BseRI | GAGGAG | 1 cut(s) | 236 |
| BseSI | GKGCMC | 1 cut(s) | 56 |
| Bsh1285I | CGRYCG | 1 cut(s) | 68 |
| BshFI | GGCC | 2 cut(s) | 83, 143 |
| BshNI | GGYRCC | 1 cut(s) | 51 |
| BshVI | ATCGAT | 1 cut(s) | 64 |
| BsiEI | CGRYCG | 1 cut(s) | 68 |
| BsmI | GAATGC | 1 cut(s) | 192 |
| BsnI | GGCC | 2 cut(s) | 83, 143 |
| Bsp1286I | GDGCHC | 1 cut(s) | 56 |
| Bsp143I | GATC | 3 cut(s) | 65, 160, 312 |
| Bsp19I | CCATGG | 1 cut(s) | 121 |
| BspACI | CCGC | 1 cut(s) | 191 |
| BspANI | GGCC | 2 cut(s) | 83, 143 |
| BspDI | ATCGAT | 1 cut(s) | 64 |
| BspHI | TCATGA | 1 cut(s) | 315 |
| BspLI | GGNNCC | 2 cut(s) | 53, 259 |
| BspPI | GGATC | 1 cut(s) | 320 |
| BspT107I | GGYRCC | 1 cut(s) | 51 |
| BsrDI | GCAATG | 1 cut(s) | 150 |
| BssECI | CCNNGG | 1 cut(s) | 121 |
| BssMI | GATC | 3 cut(s) | 65, 160, 312 |
| BssT1I | CCWWGG | 1 cut(s) | 121 |
| Bst2UI | CCWGG | 1 cut(s) | 140 |
| BstDEI | CTNAG | 1 cut(s) | 47 |
| BstDSI | CCRYGG | 1 cut(s) | 121 |
| BstKTI | GATC | 3 cut(s) | 68, 163, 315 |
| BstMBI | GATC | 3 cut(s) | 65, 160, 312 |
| BstMCI | CGRYCG | 1 cut(s) | 68 |
| BstNI | CCWGG | 1 cut(s) | 140 |
| BstSCI | CCNGG | 1 cut(s) | 138 |
| BstSLI | GKGCMC | 1 cut(s) | 56 |
| BstV2I | GAAGAC | 1 cut(s) | 141 |
| Bsu15I | ATCGAT | 1 cut(s) | 64 |
| BsuRI | GGCC | 2 cut(s) | 83, 143 |
| BsuTUI | ATCGAT | 1 cut(s) | 64 |
| BtgI | CCRYGG | 1 cut(s) | 121 |
| CciI | TCATGA | 1 cut(s) | 315 |
| ClaI | ATCGAT | 1 cut(s) | 64 |
| CviAII | CATG | 2 cut(s) | 122, 316 |
| CviJI | RGCY | 6 cut(s) | 46, 72, 83, 143, 167, 245 |
| CviKI_1 | RGCY | 6 cut(s) | 46, 72, 83, 143, 167, 245 |
| DdeI | CTNAG | 1 cut(s) | 47 |
| DpnI | GATC | 3 cut(s) | 67, 162, 314 |
| DpnII | GATC | 3 cut(s) | 65, 160, 312 |
| EaeI | YGGCCR | 2 cut(s) | 81, 141 |
| Eco130I | CCWWGG | 1 cut(s) | 121 |
| EcoRI | GAATTC | 1 cut(s) | 28 |
| EcoRII | CCWGG | 1 cut(s) | 138 |
| EcoT14I | CCWWGG | 1 cut(s) | 121 |
| ErhI | CCWWGG | 1 cut(s) | 121 |
| FaeI | CATG | 2 cut(s) | 125, 319 |
| FaiI | YATR | 2 cut(s) | 123, 317 |
| FatI | CATG | 2 cut(s) | 121, 315 |
| Fnu4HI | GCNGC | 1 cut(s) | 191 |
| Fsp4HI | GCNGC | 1 cut(s) | 191 |
| FspBI | CTAG | 2 cut(s) | 230, 239 |
| GluI | GCNGC | 1 cut(s) | 191 |
| HaeIII | GGCC | 2 cut(s) | 83, 143 |
| Hin1II | CATG | 2 cut(s) | 125, 319 |
| HindIII | AAGCTT | 1 cut(s) | 44 |
| HphI | GGTGA | 1 cut(s) | 49 |
| Hpy188I | TCNGA | 1 cut(s) | 182 |
| Hpy188III | TCNNGA | 2 cut(s) | 104, 316 |
| HpyAV | CCTTC | 2 cut(s) | 66, 124 |
| HpyF3I | CTNAG | 1 cut(s) | 47 |
| Hsp92II | CATG | 2 cut(s) | 125, 319 |
| Kzo9I | GATC | 3 cut(s) | 65, 160, 312 |
| LmnI | GCTCC | 1 cut(s) | 223 |
| LpnPI | CCDG | 5 cut(s) | 125, 149, 152, 210, 274 |
| LweI | GCATC | 1 cut(s) | 235 |
| MaeI | CTAG | 2 cut(s) | 230, 239 |
| MalI | GATC | 3 cut(s) | 67, 162, 314 |
| MboI | GATC | 3 cut(s) | 65, 160, 312 |
| MboII | GAAGA | 3 cut(s) | 119, 146, 195 |
| MhlI | GDGCHC | 1 cut(s) | 56 |
| MlsI | TGGCCA | 1 cut(s) | 143 |
| MluCI | AATT | 1 cut(s) | 28 |
| MluNI | TGGCCA | 1 cut(s) | 143 |
| MnlI | CCTC | 3 cut(s) | 204, 214, 248 |
| Mox20I | TGGCCA | 1 cut(s) | 143 |
| MscI | TGGCCA | 1 cut(s) | 143 |
| MslI | CAYNNNNRTG | 1 cut(s) | 216 |
| Msp20I | TGGCCA | 1 cut(s) | 143 |
| MspR9I | CCNGG | 1 cut(s) | 140 |
| Mva1269I | GAATGC | 1 cut(s) | 192 |
| MvaI | CCWGG | 1 cut(s) | 140 |
| NcoI | CCATGG | 1 cut(s) | 121 |
| NdeII | GATC | 3 cut(s) | 65, 160, 312 |
| NlaIII | CATG | 2 cut(s) | 125, 319 |
| NlaIV | GGNNCC | 2 cut(s) | 53, 259 |
| PagI | TCATGA | 1 cut(s) | 315 |
| PctI | GAATGC | 1 cut(s) | 192 |
| PkrI | GCNGC | 1 cut(s) | 192 |
| Ple19I | CGATCG | 1 cut(s) | 68 |
| Psp6I | CCWGG | 1 cut(s) | 138 |
| PspGI | CCWGG | 1 cut(s) | 138 |
| PspN4I | GGNNCC | 2 cut(s) | 53, 259 |
| PvuI | CGATCG | 1 cut(s) | 68 |
| RseI | CAYNNNNRTG | 1 cut(s) | 216 |
| SatI | GCNGC | 1 cut(s) | 191 |
| Sau3AI | GATC | 3 cut(s) | 65, 160, 312 |
| ScrFI | CCNGG | 1 cut(s) | 140 |
| SduI | GDGCHC | 1 cut(s) | 56 |
| SetI | ASST | 4 cut(s) | 48, 53, 141, 263 |
| SfaNI | GCATC | 1 cut(s) | 235 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 216 |
| Sse9I | AATT | 1 cut(s) | 28 |
| SsiI | CCGC | 1 cut(s) | 191 |
| SspMI | CTAG | 2 cut(s) | 230, 239 |
| StyD4I | CCNGG | 1 cut(s) | 138 |
| StyI | CCWWGG | 1 cut(s) | 121 |
| TaqI | TCGA | 1 cut(s) | 64 |
| TasI | AATT | 1 cut(s) | 28 |
| TauI | GCSGC | 1 cut(s) | 193 |
| TspDTI | ATGAA | 1 cut(s) | 224 |
| XapI | RAATTY | 1 cut(s) | 28 |
| XspI | CTAG | 2 cut(s) | 230, 239 |